Method for inactivating viruses by electromagnetic waves and application thereof

Abstract

The invention discloses a method for inactivating viruses by electromagnetic waves and application thereof, experiments prove that the inactivation effect of the electromagnetic waves on the viruses can be enhanced by heating, an experimental basis is provided for research on application conditions and methods for inactivating the viruses by electromagnetic wave radiation, and the method has a good application prospect in the aspect of virus inactivation.

Description

Technical Field

[0001] The invention relates to the fields of electromagnetics and virology, and in particular to application technology and conditions for electromagnetic wave inactivation of viruses. Background Art

[0002] Pathogenic virus infection is an important public health safety issue facing the world. Pathogenic viruses can infect a variety of hosts, including humans, causing varying degrees and types of damage or disease, or even death, which places a huge risk and burden on society. Traditional virus inactivation methods such as ultraviolet disinfection, steam disinfection, drug disinfection, ozone disinfection, etc., also have certain limitations in operability, efficiency, and pollution-free, and have disadvantages that cannot be ignored.

[0003] Electromagnetic waves have strong penetrating power, physical resonance and no pollution. Their ability to inactivate pathogenic viruses has attracted much attention, which has led to continuous exploration and development in the field of pathogenic virus inactivation. However, there is still a lack of research on the application conditions and methods of electromagnetic wave radiation inactivation of viruses.

[0004] Therefore, there is an urgent need to clarify the application conditions and methods of electromagnetic wave radiation. Summary of the invention

[0005] In view of this, in order to make up for the deficiencies of the prior art, the present invention is proposed. The purpose of the present invention is to provide a method for inactivating viruses by electromagnetic waves and its application. The present invention proves through experiments that heating can enhance the inactivation effect of electromagnetic waves on viruses, provides an experimental basis for the application conditions and methods of electromagnetic wave radiation inactivation of viruses, and has good application prospects in inactivating viruses.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions: A first aspect of the present invention provides a method for inactivating viruses, wherein the method inactivates viruses by using electromagnetic waves under heating conditions.

[0007] In the present invention, when the infectivity or pathogenicity or reproductive capacity of the virus is reduced by at least 1%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 100% (i.e., absent) relative to the reference value, the virus can be considered to be inactivated. The reference value can refer to the infectivity or pathogenicity or reproductive capacity of the virus before the inactivation operation is performed. The reference value can be an absolute value, a relative value, a value with an upper limit and / or a lower limit, a series of values, an average value, a median, a mean, or a value represented by a reference control or a reference value.

[0008] In the present invention, electromagnetic waves are electromagnetic fields that propagate in the form of waves, and include but are not limited to gamma rays, X-rays, ultraviolet rays, visible light, infrared rays, microwaves, radio waves, and sound waves. The frequency bands of electromagnetic waves are, from low to high, extremely low frequency, ultra-low frequency, very low frequency, low frequency, medium frequency, high frequency, very high frequency, ultra-high frequency, ultra-high frequency, and extremely high frequency. Among them, the extremely low frequency is 3 Hz~30 Hz, the ultra-low frequency is 30 Hz~300 Hz, the ultra-low frequency is 300 Hz~3 KHz, the very low frequency is 3 KHz~30 KHz, the low frequency is 30 KHz~300 KHz, the medium frequency is 300 KHz~3000 KHz, the high frequency is 3 MHz~30 MHz, the very high frequency is 30 MHz~300 MHz, the ultra-high frequency is 300 MHz~3 GHz, the ultra-high frequency is 3 GHz~30 GHz, and the ultra-high frequency is 30 GHz~300 GHz. The use of electromagnetic waves can inactivate the virus. Furthermore, ultra-high frequency, ultra-high frequency and extremely high frequency electromagnetic waves can be used to inactivate the virus, that is, 300 MHz~300 GHz electromagnetic waves can be used to inactivate the virus.

[0009] Furthermore, the frequency of the electromagnetic wave is 300 MHz~30 GHz.

[0010] Furthermore, the frequency of the electromagnetic wave is 1 GHz to 30 GHz.

[0011] Furthermore, the frequency of the electromagnetic wave is 10 GHz to 30 GHz.

[0012] In the present invention, the radiation power refers to the electromagnetic wave power per unit cross-sectional area perpendicular to the propagation direction, and the radiation power can be used to evaluate the intensity of the electromagnetic wave in the environment.

[0013] Furthermore, the electromagnetic wave radiation power is 1 mW / cm 2 ~1 kW / cm 2 .

[0014] Furthermore, the electromagnetic wave radiation power is 10 mW / cm 2 ~500 W / cm 2 .

[0015] Furthermore, the electromagnetic wave radiation power is 10 mW / cm 2 ~500 mW / cm 2 .

[0016] Furthermore, the electromagnetic wave radiation power is 10 mW / cm 2 ~50 mW / cm 2 .

[0017] Furthermore, the electromagnetic wave radiation power is 20 mW / cm 2 ~50 mW / cm 2 .

[0018] In the present invention, the heating condition may refer to any temperature above 20°C.

[0019] Furthermore, the temperature of the heating condition is 25°C~100°C.

[0020] Furthermore, the temperature of the heating condition is 25°C to 80°C.

[0021] Furthermore, the temperature of the heating condition is 25°C to 45°C.

[0022] In the present invention, the virus can be inactivated by heating with electromagnetic waves for more than 0 s under heating conditions, and further, the virus can be inactivated by heating for at least 0.1 s, at least 1 s, at least 2 s, at least 3 s, at least 4 s, at least 5 s, at least 6 s, at least 7 s, at least 8 s, at least 9 s, at least 10 s, at least 11 s, at least 12 s, at least 13 s, at least 14 s, at least 15 s, at least 16 s, at least 17 s, at least 18 s, at least 19 s, at least 20 s, at least 21 s, at least 22 s, at least 23 s, at least 24 s, at least 25 s, at least 26 s, at least 27 s, at least 28 s, at least 29 s, or at least 30 s.

[0023] Further, the virus is inactivated by heating for at least 1 min, at least 2 min, at least 3 min, at least 4 min, at least 5 min, at least 6 min, at least 7 min, at least 8 min, at least 9 min, at least 10 min, at least 11 min, at least 12 min, at least 13 min, at least 14 min, and at least 15 min.

[0024] Furthermore, the method includes inactivating the virus by heating at 25°C to 45°C using 10 GHz to 30 GHz electromagnetic waves.

[0025] Furthermore, the method uses 11.8 GHz electromagnetic waves to inactivate the virus at 25°C to 45°C.

[0026] Further, the method is to use 11.8 GHz, 20 mW / cm 2 ~50 mW / cm 2 Electromagnetic waves inactivate viruses at 25℃~45℃.

[0027] Further, the method is to use 11.8 GHz, 20 mW / cm2 Electromagnetic waves inactivate viruses at 25℃~45℃.

[0028] Furthermore, the heating is not less than 5 min.

[0029] Furthermore, the heating is not less than 10 min.

[0030] Furthermore, the heating is for no less than 15 min.

[0031] The viruses to be inactivated in the present invention are not particularly limited, and the viruses include enveloped viruses and non-enveloped viruses.

[0032] Further, the virus includes but is not limited to the following virus families: Retroviridae, Togaviridae, Coronaviridae, Filoviridae, Rhabdoviridae, Bunyaviridae, Orthomyxoviridae, Paramyxoviridae, Arenaviridae, Hepadnaviridae, Herpesviridae, Flaviviridae, Baculoviridae, Poxviridae or Picornaviridae.

[0033] Furthermore, the coronavirus family is selected from mouse hepatitis virus, atypical pneumonia virus, 2019 novel coronavirus or Middle East respiratory syndrome coronavirus.

[0034] Furthermore, the mouse hepatitis virus includes MHV-1, MHV-2, MHV-3, MHV-JHM, MHV-A59, MHV-Y, MHV-S, MHV-U or MHV-LIVIM.

[0035] Furthermore, the mouse hepatitis virus is selected from MHV-A59.

[0036] Furthermore, the Flaviviridae is selected from the genus Flavivirus, the genus Pestivirus or the genus Hepacivirus.

[0037] Furthermore, the Hepadnaviridae family is selected from hepatitis B virus and duck hepatitis B virus.

[0038] Furthermore, the Picornaviridae family is selected from hepatitis A virus, poliovirus, coxsackievirus or echovirus.

[0039] A second aspect of the present invention provides a method for enhancing the virus inactivation effect of electromagnetic waves, the method comprising using electromagnetic waves under heating conditions.

[0040] Furthermore, the heating temperature is 25°C to 100°C.

[0041] Furthermore, the heating temperature is 25°C to 80°C.

[0042] Furthermore, the heating temperature is 25°C to 45°C.

[0043] Furthermore, the frequency of the electromagnetic wave is 300 MHz~300 GHz.

[0044] Furthermore, the frequency of the electromagnetic wave is 300 MHz~30 GHz.

[0045] Furthermore, the frequency of the electromagnetic wave is 1 GHz to 30 GHz.

[0046] Furthermore, the frequency of the electromagnetic wave is 10 GHz to 30 GHz.

[0047] Furthermore, the frequency of the electromagnetic wave is 11.8 GHz.

[0048] Furthermore, there is a synergistic effect of inactivating viruses between 45°C and 11.8 GHz electromagnetic waves.

[0049] Furthermore, the electromagnetic wave radiation power is 1 mW / cm 2 ~1 kW / cm 2 .

[0050] Furthermore, the electromagnetic wave radiation power is 10 mW / cm 2 ~500 W / cm 2 .

[0051] Furthermore, the electromagnetic wave radiation power is 10 mW / cm 2 ~500 mW / cm 2 .

[0052] Furthermore, the electromagnetic wave radiation power is 10 mW / cm 2 ~50 mW / cm 2 .

[0053] Furthermore, the electromagnetic wave radiation power is 20 mW / cm 2 ~50 mW / cm 2 .

[0054] Furthermore, the electromagnetic wave radiation power is 20 mW / cm 2 .

[0055] A third aspect of the present invention provides a device for inactivating viruses, comprising an electromagnetic wave emission source and a heating source.

[0056] Furthermore, the electromagnetic wave emission source can emit electromagnetic waves with a frequency of 300 MHz to 300 GHz.

[0057] Furthermore, the radiation power of the electromagnetic wave emission source is 1 mW / cm 2 ~1 kW / cm 2 .

[0058] Furthermore, the heating source can heat to 25°C~100°C.

[0059] The present invention has the following advantages and beneficial effects: The present invention provides a method for inactivating viruses by electromagnetic waves and its application. The present invention proves through experiments that heating can enhance the inactivation effect of electromagnetic waves on viruses, provides an experimental basis for the study of application conditions and methods for inactivating viruses by electromagnetic wave radiation, and has good application prospects in inactivating viruses. BRIEF DESCRIPTION OF THE DRAWINGS

[0060] Figure 1 It is a host of mouse hepatitis virus - 17CL-1 cell diagram.

[0061] Figure 2 This is a graph showing the changes in virus titer after exposure to electromagnetic waves at 25 degrees Celsius for 15 minutes.

[0062] Figure 3 This is a graph showing the changes in virus titer after exposure to electromagnetic waves at 37 degrees Celsius for 15 minutes.

[0063] Figure 4 This is a graph showing the changes in virus titer after exposure to electromagnetic waves at 37 degrees Celsius for 30 minutes.

[0064] Figure 5 This is a graph showing the changes in virus titer after exposure to electromagnetic waves at 45 degrees Celsius for 15 minutes. DETAILED DESCRIPTION

[0065] The present invention is further described below in conjunction with specific examples, which are only used to explain the present invention and are not to be construed as limiting the present invention. It will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and purposes of the present invention, and that the scope of the present invention is defined by the claims and their equivalents. The experimental methods for which specific conditions are not specified in the following examples are usually tested under conventional conditions or under conditions recommended by the manufacturer.

[0066] Example 1: Host cell 17 CL-1 cell culture

[0067] 1. Experimental methods: (1) Prepare complete culture medium: 89% DMEM medium + 10% fetal bovine serum + 1% double antibody.

[0068] (2) 17 CL-1 cell recovery: Open the water bath 20 minutes in advance to preheat (37 degrees Celsius). Take out the 17CL-1 cells that were frozen in advance from the liquid nitrogen tank and immediately place them in the water bath. After the cell suspension in the cryopreservation tube is completely thawed, transfer it to a 15 ml centrifuge tube and add complete culture medium. Centrifuge at 37 degrees Celsius and 1000 rpm for 5 minutes and discard the supernatant. Add complete culture medium, pipet and mix well, transfer to a 75 square centimeter cell culture flask, add complete culture medium, label, and place in a cell culture incubator (37 degrees Celsius, 5% carbon dioxide), and observe under a microscope the next day.

[0069] (3) 17 CL-1 cell passaging: When the 17CL-1 cells in the 75 cm2 culture flask are confluent to 80%-90%, discard the old culture medium and add PBS for washing. Add 2 ml of trypsin for digestion for 2 minutes. A few cells fall off when observed with the naked eye. Immediately add complete culture medium to terminate digestion and mix with a pipette. Centrifuge at 37 degrees Celsius and 1000 rpm for 5 minutes, discard the supernatant, add complete culture medium to resuspend, divide into 75 cm2 culture flasks, add complete culture medium, mark, and observe every other day.

[0070] 2. Experimental results Cell morphology ( Figure 1 ) is good and can be used for subsequent experiments.

[0071] Example 2: Mouse hepatitis virus (MHV-A59) culture

[0072] Culture of mouse hepatitis virus (MHV-A59): Add mouse hepatitis virus (MHV-A59) solution to the monolayer or overgrown 17CL-1 cells, adsorb for 2 hours, discard the virus solution, add medium containing 2% fetal bovine serum to culture the virus, and collect the virus solution after about 48 hours when 2 / 3 of the cells show cytopathic effects. Place the culture solution containing the virus and host cells at -20 degrees Celsius and freeze and thaw three times to break the host cells, centrifuge at 3000 rpm for 15 minutes, filter through a 0.22 micron filter, collect in 1.8 ml cryotubes, and store in a -80 degrees Celsius ultra-low temperature refrigerator for later use.

[0073] Example 3: Detection of mouse hepatitis virus activity after electromagnetic wave exposure

[0074] 1. Experimental methods (1) Virus grouping and electromagnetic wave irradiation at different temperatures and exposure times ① Virus grouping and electromagnetic wave exposure (25 degrees Celsius, 15 minutes): Take out the frozen virus from the -80 degrees Celsius ultra-low temperature refrigerator, mix all the viruses thoroughly with a vortex oscillator, take 50 microliters of the virus stock solution and evenly apply it on the bottom of a 35 mm dish. A total of 16 samples were prepared, divided into a blank control group, an 11.8 GHz electromagnetic wave group (20 degrees Celsius), a 25 degrees Celsius group, and a 11.8 GHz electromagnetic wave group (25 degrees Celsius), with 4 replicates in each group. The average power density of the electromagnetic wave radiation source is 20 milliwatts per square centimeter. The prepared virus sample is covered and placed in the center of the irradiation platform for 15 minutes. The microwave irradiation source is turned off when the blank control group is irradiated; and when the blank control group and the 11.8 GHz electromagnetic wave group (20 degrees Celsius) are in the irradiation room, the incubator temperature is set to 20 degrees Celsius; when the 25 degrees Celsius group and the 11.8 GHz electromagnetic wave group (25 degrees Celsius) are in the irradiation room, the incubator temperature is set to 25 degrees Celsius. All virus samples were stored in a 4°C refrigerator when not irradiated and were always equilibrated at room temperature for 5 minutes before irradiation.

[0075] ② Virus grouping and electromagnetic wave exposure (37 degrees Celsius, 15 minutes): Take out the frozen virus from the -80 degrees Celsius ultra-low temperature refrigerator, mix all the viruses thoroughly with a vortex oscillator, take 50 microliters of the virus stock solution and evenly apply it on the bottom of a 35 mm dish. A total of 16 samples were prepared, divided into a blank control group, an 11.8 GHz electromagnetic wave group (20 degrees Celsius), a 37 degrees Celsius group, and a 11.8 GHz electromagnetic wave group (37 degrees Celsius), with 4 replicates in each group. The average power density of the electromagnetic wave radiation source is 20 milliwatts per square centimeter. The prepared virus sample is covered and placed in the center of the irradiation platform for 15 minutes. The microwave irradiation source is turned off when the blank control group is irradiated; and when the blank control group and the 11.8 GHz electromagnetic wave group (20 degrees Celsius) are in the irradiation room, the incubator temperature is set to 20 degrees Celsius; when the 37 degrees Celsius group and the 11.8 GHz electromagnetic wave group (37 degrees Celsius) are in the irradiation room, the incubator temperature is set to 37 degrees Celsius. All virus samples were stored in a 4°C refrigerator when not irradiated and were always equilibrated at room temperature for 5 minutes before irradiation.

[0076] ③ Virus grouping and electromagnetic wave exposure (37 degrees Celsius, 30 minutes): The frozen viruses were taken out from the -80°C ultra-low temperature refrigerator. All viruses were thoroughly mixed by vortex oscillator. 50 μL of virus stock solution was evenly applied to the bottom of a 35 mm dish. A total of 16 samples were prepared, divided into a blank control group, an 11.8 GHz electromagnetic wave group (20°C), a 37°C group, and a 11.8 GHz electromagnetic wave group (37°C), with 4 replicates in each group. The average power density of the electromagnetic wave radiation source was 20 milliwatts per square centimeter. The prepared virus samples were covered and placed in the center of the irradiation platform for 30 minutes. The microwave irradiation source was turned off when the blank control group was irradiated; and when the blank control group and the 11.8 GHz electromagnetic wave group (20°C) were in the irradiation room, the incubator temperature was set to 20°C; when the 37°C group and the 11.8 GHz electromagnetic wave group (37°C) were in the irradiation room, the incubator temperature was set to 37°C. All virus samples were stored in a 4°C refrigerator when not irradiated and were always equilibrated at room temperature for 5 minutes before irradiation.

[0077] ④Virus grouping and electromagnetic wave exposure (45 degrees Celsius, 15 minutes): The frozen viruses were taken out from the -80°C ultra-low temperature refrigerator. All viruses were thoroughly mixed by vortex oscillator. 50 μL of virus stock solution was evenly applied to the bottom of a 35 mm dish. A total of 16 samples were prepared, divided into a blank control group, an 11.8 GHz electromagnetic wave group (20°C), a 45°C group, and a 11.8 GHz electromagnetic wave group (45°C), with 4 replicates in each group. The average power density of the electromagnetic wave radiation source was 20 milliwatts per square centimeter. The prepared virus samples were covered and placed in the center of the irradiation platform for 15 minutes. The microwave irradiation source was turned off when the blank control group was irradiated; and when the blank control group and the 11.8 GHz electromagnetic wave group (20°C) were in the irradiation room, the incubator temperature was set to 20°C; when the 45°C group and the 11.8 GHz electromagnetic wave group (45°C) were in the irradiation room, the incubator temperature was set to 45°C. All virus samples were stored in a 4°C refrigerator when not irradiated and were always equilibrated at room temperature for 5 minutes before irradiation.

[0078] (2) Mouse hepatitis virus (MHV-A59) titer determination: The virus stock solution was diluted 10 times with a culture medium containing 5% fetal bovine serum. A 96-well cell culture plate with a monolayer of 17CL-1 cells was taken. Four replicate wells were made for each dilution and a negative control was set. The cells were placed in a 37°C, 5% carbon dioxide incubator for continuous culture. After 24 and 36 hours, the cells were taken out and observed under a microscope, and the cell pathological changes were recorded well by well. The virus titer was calculated (Reed-Muench method) as the half-cell infection dose.

[0079] The logarithm of the 50% cell infection dose = the logarithm of the dilution of the group with a pathology rate higher than 50% + the distance ratio.

[0080] Distance ratio = (lesion rate of the group above 50% - 50) / (lesion rate of the group above 50% - lesion rate of the group below 50%).

[0081] 2. Experimental results (1) At 25 degrees Celsius, after irradiating mouse hepatitis virus with 11.8 GHz electromagnetic waves at 20 milliwatts per square centimeter for 15 minutes, the virus infectivity changes as follows: Figure 2 The results showed that compared with the blank control group, the virus infectivity in the 11.8 GHz electromagnetic wave (25 degrees Celsius) group was significantly reduced (P<0.05). This indicates that exposing mouse hepatitis virus to 11.8 GHz electromagnetic waves at 20 milliwatts per square centimeter for 15 minutes at 25 degrees Celsius can reduce its infectivity.

[0082] (2) At 37 degrees Celsius, after irradiating mouse hepatitis virus with 11.8 GHz electromagnetic waves at 20 milliwatts per square centimeter for 15 minutes, the virus infectivity changes as follows: Figure 3 The results showed that compared with the blank control group, the virus infectivity in the 11.8 GHz electromagnetic wave (37 degrees Celsius) group was significantly reduced (P<0.001), and the virus titer was reduced by more than 1 logarithm; and compared with the 11.8 GHz electromagnetic wave (20 degrees Celsius) group and the 37 degrees Celsius group, the virus infectivity in the 11.8 GHz electromagnetic wave (37 degrees Celsius) group was also significantly reduced (P<0.001). This shows that exposing mouse hepatitis virus to 11.8 GHz electromagnetic waves at 20 milliwatts per square centimeter for 15 minutes at 37 degrees Celsius can reduce its infectivity.

[0083] (3) At 37 degrees Celsius, after irradiating mouse hepatitis virus with 11.8 GHz electromagnetic waves at 20 milliwatts per square centimeter for 30 minutes, the virus infectivity changes as follows: Figure 4 . The results showed that compared with the blank control group, the virus infectivity of the 37 degrees Celsius group and the 11.8 GHz electromagnetic wave (37 degrees Celsius) group was significantly reduced (P<0.001), and the virus titer of the 11.8 GHz electromagnetic wave (37 degrees Celsius) group was reduced by more than 3 logarithms; compared with the 37 degrees Celsius group, the virus infectivity of the 11.8 GHz electromagnetic wave (37 degrees Celsius) group was also significantly reduced (P<0.01). This shows that exposing mouse hepatitis virus to 11.8 GHz electromagnetic waves at 20 milliwatts per square centimeter for 30 minutes at 37 degrees Celsius can reduce its infectivity. And compared with normal temperature (20 degrees Celsius), exposure to electromagnetic waves for 30 minutes at 37 degrees Celsius can more prominently highlight the inactivation effect of electromagnetic waves on the virus.

[0084] (4) At 45 degrees Celsius, after irradiating mouse hepatitis virus with 11.8 GHz electromagnetic waves at 20 milliwatts per square centimeter for 15 minutes, the virus infectivity changes as follows: Figure 4 . The results showed that compared with the blank control group, the virus infectivity in the 11.8 GHz electromagnetic wave (45 degrees Celsius) group was significantly reduced (P<0.001), and the virus titer was reduced by more than 5 logarithms, reaching the virus inactivation standard required by disinfection; and compared with the 45 degrees Celsius group, the virus infectivity in the 11.8 GHz electromagnetic wave (45 degrees Celsius) group was also significantly reduced (P<0.001). This shows that exposing mouse hepatitis virus to 11.8 GHz electromagnetic waves at 20 milliwatts per square centimeter for 15 minutes at 45 degrees Celsius can reduce its infectivity. And statistical analysis found that there is a synergistic effect of inactivating viruses between 45 degrees Celsius and 11.8 GHz electromagnetic waves (P<0.05).

[0085] The description of the above embodiments is only used to understand the method and core idea of ​​the present invention. It should be pointed out that, for those skilled in the art, several improvements and modifications can be made to the present invention without departing from the principle of the present invention, and these improvements and modifications will also fall within the scope of protection of the claims of the present invention.

Claims

1. A method for inactivating viruses, characterized in that: The method inactivates the virus by using electromagnetic waves under heating conditions.

2. The method according to claim 1, characterized in that The electromagnetic wave frequency is 300 MHz to 300 GHz; Preferably, the frequency of the electromagnetic wave is 300 MHz to 30 GHz; Preferably, the frequency of the electromagnetic wave is 1 GHz to 30 GHz; Preferably, the frequency of the electromagnetic wave is 10 GHz to 30 GHz.

3. The method according to claim 1, characterized in that The electromagnetic wave radiation power is 1 mW / cm 2 ~1 kW / cm 2 ; Preferably, the electromagnetic wave radiation power is 10 mW / cm 2 ~500 W / cm 2 ; Preferably, the electromagnetic wave radiation power is 10 mW / cm 2 ~500 mW / cm 2 ; Preferably, the electromagnetic wave radiation power is 10 mW / cm 2 ~50 mW / cm 2 ; Preferably, the electromagnetic wave radiation power is 20 mW / cm 2 ~50 mW / cm 2 .

4. The method according to claim 1, characterized in that: The temperature of the heating condition is 25°C to 100°C; Preferably, the temperature of the heating condition is 25°C to 80°C; Preferably, the temperature of the heating condition is 25°C~45°C.

5. The method according to claim 1, characterized in that The method inactivates the virus by heating with electromagnetic waves for at least 5 minutes under heating conditions; Preferably, the method inactivates the virus by heating with electromagnetic waves for at least 10 min under heating conditions; Preferably, the method inactivates the virus by heating with electromagnetic waves for at least 15 minutes under heating conditions.

6. The method according to any one of claims 1 to 5, characterized in that The method comprises using 10 GHz to 30 GHz electromagnetic waves to heat and inactivate the virus at 25°C to 45°C; Preferably, the method is to inactivate the virus using 11.8 GHz electromagnetic waves at 25°C to 45°C; Preferably, the method is to use 11.8 GHz, 20 mW / cm 2 ~50 mW / cm 2 Electromagnetic waves inactivate viruses at 25°C to 45°C; Preferably, the method is to use 11.8 GHz, 20 mW / cm 2 Electromagnetic waves inactivate viruses at 25℃~45℃.

7. The method according to claim 6, characterized in that The heating is not less than 5 minutes; Preferably, the heating is not less than 10 min; Preferably, the heating is for no less than 15 min.

8. The method according to claim 1, characterized in that The virus includes the following virus families: Retroviridae, Togaviridae, Coronaviridae, Filoviridae, Rhabdoviridae, Bunyaviridae, Orthomyxoviridae, Paramyxoviridae, Arenaviridae, Hepadnaviridae, Herpesviridae, Flaviviridae, Baculoviridae, Poxviridae or Picornaviridae; Preferably, the coronavirus family is selected from mouse hepatitis virus, SARS virus, 2019 novel coronavirus or Middle East respiratory syndrome coronavirus; Preferably, the mouse hepatitis virus comprises MHV-1, MHV-2, MHV-3, MHV-JHM, MHV-A59, MHV-Y, MHV-S, MHV-U or MHV-LIVIM; Preferably, the mouse hepatitis virus is selected from MHV-A59; Preferably, the Flaviviridae is selected from the genus Flavivirus, Pestivirus or Hepacivirus; Preferably, the Hepadnaviridae is selected from the group consisting of hepatitis B virus and duck hepatitis B virus; Preferably, the Picornaviridae is selected from Hepatitis A virus, Poliovirus, Coxsackievirus or Echovirus.

9. A method for enhancing the inactivation of viruses by electromagnetic waves, characterized in that: The method comprises using electromagnetic waves under heating conditions; Preferably, the heating temperature is 25°C to 100°C; Preferably, the heating temperature is 25°C to 80°C; Preferably, the heating temperature is 25°C to 45°C; Preferably, the frequency of the electromagnetic wave is 300 MHz to 300 GHz; Preferably, the frequency of the electromagnetic wave is 10 GHz to 30 GHz; Preferably, the frequency of the electromagnetic wave is 11.8 GHz; Preferably, the electromagnetic wave radiation power is 1 mW / cm 2 ~1 kW / cm 2 ; Preferably, the electromagnetic wave radiation power is 20 mW / cm 2 ~50 mW / cm 2 ; Preferably, the electromagnetic wave radiation power is 20 mW / cm 2 .

10. A device for inactivating viruses, characterized in that: The device comprises an electromagnetic wave emission source and a heating source; Preferably, the electromagnetic wave emission source can emit electromagnetic waves with a frequency of 300 MHz to 300 GHz; Preferably, the radiation power of the electromagnetic wave emission source is 1 mW / cm 2 ~1 kW / cm 2 ; Preferably, the heating source can heat to 25°C~100°C.

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