A microbial flora mother liquor culture device and a culture method

Abstract

The present invention relates to the field of microbial culture, and discloses a microbial flora mother liquor culture device and a culture method, including a water tank, a culture chamber, and an oxygen supply device. The water tank is communicated with the culture chamber through a water pipe. A biological filler box is installed in the culture chamber. A plurality of the biological filler boxes are arranged in a ring shape and are arranged in an inner and outer layer. The center of the culture chamber is a cavity. By providing the inner and outer layer biological filler boxes, the present invention can increase the attachment area and permeability of microorganisms, thereby increasing the reproduction quantity of microorganisms. Moreover, for the biological filler box with a frame structure, biological fillers are filled between the outer shell and the inner shell, which can effectively improve the demoulding efficiency of the microbial flora, and further increase the density of the microbial flora in the sewage. Furthermore, the demoulding assembly can make the biological filler box rotate and reciprocate, thereby further improving the demoulding efficiency of the microbial flora and enhancing the sewage purification efficiency.

Description

Technical Field

[0001] The present invention relates to the field of microbial culture, and particularly to a device and a method for culturing a microbial flora mother liquor. Background Art

[0002] With the development of the times, the use of microbial flora for water treatment has been widely applied. The existing method is to supply gas and oxygen through an oxygen supply device and a water supply device to enable microorganisms to form a biofilm and reproduce on a biological filler, and then to remove the biofilm, and continuously output a microbial flora mother liquor containing various microorganisms to the surrounding polluted water body. The microbial flora mother liquor in the polluted water body undergoes nitrification and denitrification reactions and degradation of various suspended solids, organic matters, and various harmful pollutants, so as to achieve the purification of sewage by microorganisms.

[0003] There are still many problems in the above treatment methods. For example: 1. During the use of the existing biological filler, microorganisms reproduce and remove the biofilm, but the biofilm removal rate is low, resulting in a low density of the microbial mother liquor in water and reducing the sewage purification effect. 2. Currently, the microbial mother liquor is usually discharged through an overflow pipe and enters the sewage. The amount of the gradually overflowing mother liquor is small and the penetration rate in the sewage is slow. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide a device for culturing a microbial flora mother liquor, which changes the filling method, the filling structure, and the discharging method of the microbial flora mother liquor, improves the biofilm removal rate and the content of the microbial flora mother liquor in sewage, and further improves the sewage purification efficiency.

[0005] A device for culturing a microbial flora mother liquor according to the present invention includes a water tank, a culture chamber, and an oxygen supply device. The water tank is communicated with the culture chamber through a water pipe. A biological filler box is installed in the culture chamber. A plurality of the biological filler boxes are arranged in a ring and are arranged in two layers, inner and outer. A cavity is formed in the center of the culture chamber. The upper and lower ends of the biological filler box are respectively clamped on two symmetrically arranged fixing components. The biological filler box includes an outer shell and an inner shell. Biological filler is filled between the outer shell and the inner shell. The outer shell is a frame structure, and rolling frames for removing the biofilm are installed around the frame. Conical through holes are formed around the inner shell.

[0006] The fixing component includes three annular pipes and brackets. The three annular pipes are fixedly connected by a plurality of the brackets. The oxygen supply device is fixed and communicated with the annular pipes. An automatic water supply and drainage mechanism is further provided in the culture chamber. A collection chamber is arranged on one side of the culture chamber. The collection chamber collects the sewage in the culture chamber through the automatic water supply and drainage mechanism.

[0007] The annular pipe is connected with a biofilm removal component, and the biofilm removal component rotates and reciprocally shakes the biological filler box to remove the biofilm of microorganisms.

[0008] A microbial flora mother liquor culture device of the present invention, wherein the demoulding assembly includes a lifting assembly and a vibration assembly. The vibration assembly includes a motor. The mounting plate of the motor is fixed to the lifting assembly. The output end of the motor is coaxially fixed to a first rotating shaft. The first rotating shaft is fixed to a first disc. A first guiding slider is fixed on the first disc. The first guiding slider is slidably connected to a first guiding track. The first guiding slider is perpendicularly fixed to a second guiding track. The second guiding track is slidably connected to a second guiding slider. The second guiding slider is slidably connected to a second disc. The second disc is coaxially fixed to a second rotating shaft. An eccentric block is fixed on the second rotating shaft. The second rotating shaft is connected to a third slider by a bearing. The third slider moves along a rectangular frame. The rectangular frame is fixedly connected to a first support rod. The first support rod is fixed to the mounting plate of the motor. Both sides of the third slider are respectively fixed to one end of a first spring and a second spring. The first spring and the second spring are fixed to the rectangular frame. The second rotating shaft is fixed to a second support rod. The second support rod is fixed to the annular pipe.

[0009] A microbial flora mother liquor culture device of the present invention, wherein the lifting assembly includes a cylinder. The cylinder is fixed to a support frame. The output end of the cylinder is fixed to the mounting plate of the motor.

[0010] A microbial flora mother liquor culture device of the present invention, wherein both ends of the outer shell and the inner shell are respectively clamped to a plug plate. Leakage holes are evenly opened on the plug plate. Mounting holes are opened on the periphery of the outer shell. A rolling frame is arranged on each mounting hole. The rolling frame includes a corrugated plate and a rotating wheel. A plurality of the corrugated plates and the rotating wheels are alternately arranged on the mounting hole. The corrugated plate and the rotating wheel are rotatably connected to the inner wall of the mounting hole.

[0011] A microbial flora mother liquor culture device of the present invention, wherein the rotating wheel includes a rotating shaft and fan blades. A plurality of the fan blades are fixed on the rotating shaft.

[0012] A microbial flora mother liquor culture device of the present invention, wherein the automatic water supply and drainage mechanism includes a lifting plate. An annular groove is opened on the lifting plate. The annular pipe is attached to the annular groove. The lifting plate is fixed to a third support rod. The third support rod is fixed to the rectangular frame. A water inlet hole is opened at the center of the lifting plate. The water inlet hole is rotatably connected to a baffle.

[0013] A microbial flora mother liquor culture device of the present invention, wherein the oxygen supply device includes an oxygen supply pipe. The oxygen supply pipe is in a C shape. The upper and lower sides of the oxygen supply pipe are respectively fixed and communicated with the annular pipes at the top and the bottom. The oxygen supply pipe is communicated with an oxygen supply machine.

[0014] A method for culturing a microbial flora mother liquor according to the present invention comprises the following steps:

[0015] S1. First, sewage flows from a water tank into a culture chamber;

[0016] S2. Start a lifting assembly, place a biological packing box into the culture chamber, fill the space around the biological packing box with sewage, and the microorganisms in the sewage start to form a biofilm and multiply;

[0017] S3. Detect the concentration of microorganisms in the culture chamber, and prepare to discharge the biofilm and the microbial flora mother liquor;

[0018] S4. Start a biofilm removal assembly, rotate and reciprocate the biological packing box to achieve biofilm removal, and lift the biological packing box out of the culture chamber. During the lifting process, the microbial flora mother liquor overflows into a collection chamber, and through an automatic water supply and drainage mechanism, the culture chamber is replenished with sewage;

[0019] S5. The water tank is replenished with sewage.

[0020] The differences between a microbial flora mother liquor culture device and a culture method according to the present invention and the prior art are as follows:

[0021] 1. By providing an inner and outer layer of biological packing boxes, the present invention can increase the attachment area and permeability of microorganisms, thereby increasing the number of microorganisms multiplying. Moreover, for the biological packing box with a frame structure, biological packing is filled between the outer shell and the inner shell, which can effectively improve the biofilm removal efficiency of the microbial flora, and further increase the density of the microbial flora in the sewage. Furthermore, the biofilm removal assembly can rotate and reciprocate the biological packing box, thereby further improving the biofilm removal efficiency of the microbial flora and enhancing the sewage purification efficiency.

[0022] 2. Through the rolling frames provided on the outer shell, the wave plates in the present invention provide attachment space and growth space for microorganisms, and during the lifting process, relying on the thrust of the water flow, the rotating wheels can rotate self - sufficiently, generating turbulence in the sewage, improving the biofilm removal efficiency, and enabling the rapid diffusion of the biological flora.

[0023] The following further describes a microbial flora mother liquor culture device and a culture method according to the present invention with reference to the accompanying drawings. Description of the Drawings

[0024] Figure 1 is the front view of a microbial flora mother liquor culture device and a culture method;

[0025] Figure 2 is Figure 1 the axonometric view of the microbial flora mother liquor culture device and the culture method shown;

[0026] Figure 3 is Figure 1 the partial exploded view of...

[0027] Figure 4 is an axonometric view of a biological packing box;

[0028] Figure 5 is Figure 4 an exploded view of;

[0029] Figure 6 is a structural schematic diagram of a rolling frame;

[0030] Figure 7 is Figure 6 a partially enlarged view of. Specific embodiments

[0031] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0032] Please refer to Figure 1 - Figure 7 , the present invention provides a technical solution, which specifically includes the following embodiments:

[0033] Embodiment 1:

[0034] A microbial flora mother liquor culture device includes a water tank 1, a culture chamber 2, and an oxygen supply device 3. The water tank 1 is communicated with the culture chamber 2 through a water pipe 11. Sewage is stored in the water tank 1 and can be supplemented with sewage from the outside at any time through a water pump. A one-way valve is installed on the water pipe 11 to prevent the sewage in the culture chamber 2 from flowing back. A biological packing box 4 is installed in the culture chamber 2. A plurality of the biological packing boxes 4 are arranged in a ring and are arranged in two inner and outer layers. The center of the culture chamber 2 is a cavity, so that it is easier for the microbial flora to strip the film. The upper and lower ends of the biological packing box 4 are respectively clamped on two symmetrically arranged fixing components 5. The biological packing box 4 includes an outer shell 41 and an inner shell 42. The inner shell 42 is sleeved inside the outer shell 41. Biological packing is filled between the outer shell 41 and the inner shell 42. The outer shell 41 is a frame structure, and a rolling frame 401 for film stripping is installed around the frame. Conical through holes 402 are opened around the inner shell 42. The conical through holes 402 are large in the direction close to the outer shell and small in the direction away from the outer shell, which can prevent large particles in the sewage from entering the packing and extend the service life of the biological packing.

[0035] The fixed component 5 includes three annular tubes 51 and a bracket 52. The three annular tubes 51 are fixedly connected by a plurality of the brackets 52. The bracket 52 is also annularly arranged. The oxygen supply device 3 is fixed and communicated with the annular tube 51. The inside of the annular tube 51 is hollow and provided with air holes for releasing oxygen. The culture chamber 2 is further provided with an automatic water supply and drainage mechanism 7. A collection chamber 6 is arranged on one side of the culture chamber 2. The collection chamber 6 collects the sewage in the culture chamber 2 through the automatic water supply and drainage mechanism 7;

[0036] The annular tube 51 is connected to the demembranation component 8. The demembranation component 8 rotates and reciprocates the biological packing box 4 to demembranate the microorganisms.

[0037] During operation, the sewage flows from the water tank 1 into the culture chamber 2. The microorganisms in the sewage adhere to the biological packing box 4. After the oxygen supply device 3 supplies oxygen through the annular tube 51 and a certain period of reproduction, the demembranation component 8 is started to rotate and reciprocate the biological packing box 4, so that the microbial flora demembranes. The formed microbial flora mother liquor can quickly and evenly penetrate into the sewage, thereby reacting and degrading the harmful pollutants in the sewage, purifying the sewage. And the sewage containing the microbial flora mother liquor in the culture chamber 2 flows into the collection chamber 6 through the automatic water supply and drainage mechanism 7. At the same time, new sewage is poured into the culture chamber 2 to re-culture the microbial flora mother liquor, ensuring the stable content of the microbial flora mother liquor in the sewage.

[0038] By arranging the inner and outer layers of the biological packing box 4, the present invention can increase the attachment area and permeability of the microorganisms, thereby increasing the reproduction quantity of the microorganisms. And for the biological packing box 4 with a frame structure, the biological packing is filled between the outer shell 41 and the inner shell 42, which can effectively improve the demembranation efficiency of the microbial flora, and further increase the density of the microbial flora in the sewage. Moreover, the demembranation component 8 can rotate and reciprocate the biological packing box 4, thereby further improving the demembranation efficiency of the microbial flora and enhancing the sewage purification efficiency.

[0039] As a further explanation of this embodiment, the demoulding assembly 8 includes a lifting assembly 9 and a vibration assembly 80. The vibration assembly 80 includes a motor 801. The mounting plate of the motor 801 is fixed to the lifting assembly 9. The output end of the motor 801 is coaxially fixed to a first rotating shaft 802. The first rotating shaft 802 is fixed to a first disc 803. A first guiding slider 804 is fixed to the first disc 803. The first guiding slider 804 is slidably connected to a first guiding track 805. The first guiding slider 804 is perpendicularly fixed to a second guiding track 806. The second guiding track 806 is slidably connected to a second guiding slider 807. The second guiding slider 807 is slidably connected to a second disc 816. The second disc 816 is coaxially fixed to a second rotating shaft 808. An eccentric block 809 is fixed to the second rotating shaft 808. The second rotating shaft 808 is connected to a third slider 810 by a bearing. The third slider 810 moves along a rectangular frame 811. The rectangular frame 811 is fixedly connected to a first support rod 812. The first support rod 812 is fixed to the mounting plate of the motor 801. Both sides of the third slider 810 are respectively fixed to one ends of a first spring 813 and a second spring 814. The first spring 813 and the second spring 814 are fixed to the rectangular frame 811. The second rotating shaft 808 is fixed to a second support rod 815. The second support rod 815 is fixed to the annular pipe 51.

[0040] The lifting assembly 9 includes a cylinder 91. The cylinder 91 is fixed to a support frame 92. The output end of the cylinder 91 is fixed to the mounting plate of the motor 801.

[0041] During operation, when the microbial flora is demoulded, the motor 801 is started to drive the first rotating shaft 802 to rotate, so that the first disc 803 drives the second disc 816 to rotate, and then the second rotating shaft 808 rotates, causing the eccentric block 809 to rotate, making the third slider 810 reciprocate along the rectangular frame 811. Thus, while the second rotating shaft 808 rotates, it also reciprocates, so that the biological filler box 4 rotates and reciprocates at the same time, causing the microbial flora to be demoulded. And after the demoulding assembly 8 works for a period of time, the lifting assembly 9 is started to make the cylinder 91 drive the support frame 92 to lift, driving the demoulding assembly to rise and lifting the biological filler box 4 out of the culture tank 2. During the lifting process, the microbial flora can fall off more thoroughly, and during the lifting process, the penetration of the microbial flora into the sewage is accelerated, improving the sewage purification efficiency.

[0042] Among them, a dust-proof shell is sleeved outside the culture tank 2 and the demoulding assembly 8.

[0043] As a further explanation of this embodiment, both ends of the outer shell 41 and the inner shell 42 are respectively clamped with the plug plate 403. Leakage holes 404 are evenly opened on the plug plate 403. Mounting holes 405 are opened around the outer shell 41. The rolling frame 401 is arranged on each mounting hole 405. The rolling frame 401 includes a corrugated plate 4001 and a rotating wheel 4002. A plurality of the corrugated plates 4001 and the rotating wheels 4002 are alternately arranged on the mounting hole 405. The corrugated plate 4001 and the rotating wheel 4002 are rotatably connected to the inner wall of the mounting hole 405. The rotating wheel 4002 includes a rotating shaft 4003 and fan blades 4004. A plurality of the fan blades 4004 are fixed on the rotating shaft 4003.

[0044] In the present invention, through the rolling frame 401 provided on the outer shell 41, the corrugated plate 4001 provides an attachment space and a growth space for microorganisms. And during the lifting process, relying on the thrust of the water flow, the rotating wheel 4002 can rotate self - sufficiently, generating turbulence in the sewage, improving the film - stripping efficiency, and enabling the rapid diffusion of the biological flora.

[0045] As a further explanation of this example, the automatic water supply and drainage mechanism 7 includes a lifting plate 71. An annular groove 72 is opened on the lifting plate 71. The annular pipe 51 is attached to the annular groove 72. The lifting plate 71 is fixed to the third support rod 73. The third support rod 73 is fixed to the rectangular frame 811. An inlet hole 75 is opened at the center of the lifting plate 71. The inlet hole 75 is rotatably connected to the baffle 74.

[0046] During operation, the lifting plate 71 can move upward through the lifting of the third support rod 73. During the process of lifting the biological packing box 4 out of the culture bin 2, the lifting plate 71 lifts the biological flora mother liquor into the collection bin 6. The lifting plate 71 divides the culture bin 2 into two parts. During the lifting process, the upper part of the lifting plate 71 is the biological flora mother liquor, and the lower part of the lifting plate 71 is sewage. The sewage gradually flows in from the water tank 1, and the biological flora mother liquor gradually enters the collection bin 6. When the biological packing box 4 is lifted out of the culture bin 2, the sewage occupies the original position of the biological flora mother liquor, and all the biological flora mother liquor enters the collection bin 6. When the biological packing box 4 falls, the baffle 74 is opened by the pressure of the sewage and fills the surrounding of the biological packing box 4.

[0047] Among them, the space of the annular groove 72 allows the lifting plate 71 to rotate and sway left and right.

[0048] As a further explanation of this example, the oxygen - supply device 3 includes an oxygen - supply pipe 30. The oxygen - supply pipe 30 is in a C - shape. The upper and lower sides of the oxygen - supply pipe 30 are respectively fixed and communicated with the annular pipes 51 at the top and the bottom. The oxygen - supply pipe 30 is communicated with an oxygen - supply machine.

[0049] The present invention oxygenates the upper and lower annular pipes 51 through the oxygen supply pipe 30, enabling the uniform diffusion of oxygen in the sewage, which can improve the survival rate of microorganisms and thus enhance the reproduction efficiency.

[0050] A method for culturing a microbial flora mother liquor includes the following steps:

[0051] S1. First, flow the sewage from the water tank 1 into the culture chamber 2.

[0052] S2. Start the lifting assembly 9, place the biological packing box 4 into the culture chamber 2, fill the sewage around the biological packing box 4, and the microorganisms in the sewage start to form a film and reproduce.

[0053] S3. Detect the concentration of microorganisms in the culture chamber 2 and prepare to discharge the film and the microbial flora mother liquor.

[0054] S4. Start the film removal assembly 8 to rotate and reciprocally vibrate the biological packing box 4 to achieve film removal, and lift the biological packing box 4 out of the culture chamber 2. During the lifting process, the microbial flora mother liquor overflows into the collection chamber 6, and through the automatic water supply and drainage mechanism 7, the culture chamber 2 is replenished with sewage.

[0055] S5. The water tank 1 is replenished with sewage.

Claims

1. A microbial flora mother liquid culture device, comprising a water tank, a culture chamber, and an oxygen supply device, characterized in that: The water tank is connected to the culture bin through a water pipe. A biological stuffing box is installed in the culture bin. A plurality of biological stuffing boxes are arranged in a ring shape and are arranged in two layers, the center of the culture bin is a cavity. The upper and lower ends of the biological stuffing box are respectively clamped on two symmetrically arranged fixing components. The biological stuffing box includes an outer shell and an inner shell. The outer shell and the inner shell are filled with biological stuffing. The outer shell is a frame structure. A rolling frame for film removal is installed around the frame. The rolling frame includes a wave plate and a rotating wheel. A plurality of the wave plates and the rotating wheels are alternately arranged and rotatably arranged on the outer shell. Conical through holes are provided around the inner shell. The fixing assembly includes three annular tubes and brackets, the three annular tubes are fixedly connected by a plurality of brackets, the oxygen supply device is fixed and connected to the annular tubes; the culture bin is also provided with an automatic water supply and drainage mechanism, the automatic water supply and drainage mechanism includes a lifting plate, a water inlet hole is provided in the center of the lifting plate, the water inlet hole is rotatably connected to the baffle plate, a collecting bin is provided on one side of the culture bin, when the biological stuffing box is lifted out of the culture bin, the lifting plate lifts the biological flora mother liquid to the collecting bin, the lifting plate divides the culture bin into two parts, when the biological stuffing box falls, the baffle plate is opened by the pressure of the sewage, and fills the surrounding of the biological stuffing box; The annular tube is connected to a film removal component, and the film removal component causes the biological filling box to rotate and reciprocate to remove the microorganisms. The film removal component includes a lifting component and a vibration component.

2. A microbial flora mother liquid culture device according to claim 1, characterized in that: The vibration assembly includes a motor, a mounting plate of the motor is fixed to the lifting assembly, an output end of the motor is coaxially fixed to the first rotating shaft, the first rotating shaft is fixed to the first disc, a first guide slider is fixed on the first disc, the first guide slider is slidably connected to the first guide rail, the first guide slider is vertically fixed to the second guide rail, the second guide rail is slidably connected to the second guide slider, the second guide slider is slidably connected to the second disc, the second disc is coaxially fixed to the second rotating shaft, an eccentric block is fixed on the second rotating shaft, the second rotating shaft is connected to the third slider bearing, the third slider moves along the rectangular frame, the rectangular frame is fixedly connected to the first support rod, the first support rod is fixed to the mounting plate of the motor, two sides of the third slider are respectively fixed to one end of the first spring and the second spring, the first spring and the second spring are fixed to the rectangular frame, the second rotating shaft is fixed to the second support rod, and the second support rod is fixed to the annular tube.

3. A microbial flora mother liquid culture device according to claim 2, characterized in that: The lifting assembly includes a cylinder, the cylinder is fixed to the support frame, and the output end of the cylinder is fixed to the mounting plate of the motor.

4. A microbial flora mother liquid culture device according to claim 1, characterized in that: The two ends of the outer shell and the inner shell are respectively clamped with the blocking plates, the blocking plates are evenly provided with water leakage holes, the outer shell is provided with mounting holes around, each mounting hole is provided with the rolling frame, a plurality of the wave plates and the rotating wheels are alternately arranged on the mounting holes, and the wave plates and the rotating wheels are rotatably connected to the inner walls of the mounting holes.

5. A microbial flora mother liquid culture device according to claim 4, characterized in that: The rotating wheel comprises a rotating shaft and blades, and a plurality of blades are fixed on the rotating shaft.

6. A microbial flora mother liquid culture device according to claim 2, characterized in that: The lifting plate is provided with an annular groove, the annular tube is attached to the annular groove, the lifting plate is fixed to the third support rod, and the third support rod is fixed to the rectangular frame.

7. A microbial flora mother liquid culture device according to claim 6, characterized in that: The oxygen supply device comprises an oxygen supply pipe, which is C-shaped. The upper and lower sides of the oxygen supply pipe are respectively fixed to and connected with the annular pipes at the top and bottom, and the oxygen supply pipe is connected with the oxygen supply machine.

8. A method for culturing a microbial flora mother solution, based on a microbial flora mother solution culturing device according to any one of claims 1 to 7, characterized in that: The steps include: S1, firstly, the sewage flows from the water tank into the culture chamber; S2, start the lifting component, put the biological filler box into the culture chamber, the sewage fills the area around the biological filler box, and the microorganisms in the sewage begin to form biofilms and reproduce; S3, detecting the concentration of microorganisms in the culture chamber, preparing to discharge the film and discharge the mother liquor of the microbial flora; S4, start the film removal component, make the biological stuffing box rotate and reciprocate to achieve film removal, and lift the biological stuffing box out of the culture chamber. During the lifting process, the mother liquid of the microbial flora overflows into the collection chamber, and the culture chamber is refilled with sewage through the automatic water supply and drainage mechanism; S5, the water tank is replenished with sewage.

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