Cell filtration device and filtration method thereof

Abstract

The present application relates to the technical field of cell filtration, and provides a cell filtration device and a filtration method thereof, wherein the cell filtration device comprises: a first filtration part, the first filtration part is used to receive a reaction reagent and a cell suspension, and separate the impurities in the cell suspension after mixing the reaction reagent and the cell suspension; a second filtration part, the second filtration part comprises a filter element and a plurality of detection elements, the filter element is used to filter the cell suspension, and the detection element is used to obtain the force applied to the filter element during filtration; and a clearing part, which is arranged on the other end of the second filtration part, and is used to clear the blockage when the filter element is blocked. The cell filtration device provided by the present application can improve the technical problem existing in the related art that the working efficiency of the cell filtration device is reduced by manually replacing or cleaning the blocked filter net.

Description

Technical Field

[0001] The present application relates to the field of cell filtration technology, and in particular to a cell filtration device and a filtration method thereof. Background Art

[0002] Cell filtration devices are instruments used to separate and filter biological molecules or cells. They are widely used in many fields such as cell culture, biopharmaceuticals, biotechnology research, food and beverage industry, and water treatment.

[0003] When the cell filtration device in the related art continuously filters a large amount of cell suspension for a long time, the filter screen is prone to clogging. At this time, it is usually necessary to stop the operation of the cell filtration device and manually replace or clean the filter screen, resulting in reduced work efficiency. Summary of the invention

[0004] The embodiment of the present application provides a cell filtration device and a filtration method thereof, which can improve the technical problem existing in the related art that the clogged filter screen needs to be manually replaced or cleaned, which reduces the working efficiency of the cell filtration device.

[0005] In a first aspect, an embodiment of the present application provides a cell filtration device, comprising:

[0006] A first filter portion, the first filter portion having a first containing space, and the first filter portion is used to separate impurities in the cell suspension;

[0007] A second filter portion, one end of which is connected to the first filter portion, the second filter portion having a second accommodating space, the second accommodating space being communicated with the first accommodating space, the second filter portion comprising a filter element and a plurality of detection elements, the filter element being used to filter the cell suspension, the detection elements being used to obtain the force exerted on the filter element during filtering; and a clearing portion, which is arranged on the other end of the second filter portion, the clearing portion having a third accommodating space, the other end portion of the second filter portion being located in the third accommodating space, the third accommodating space being communicated with the second accommodating space, the clearing portion being used to clear the blockage when the filter element is blocked.

[0008] The above technical solutions in the embodiments of the present application have at least the following technical effects:

[0009] The cell filtration device provided in the embodiment of the present application receives the reaction reagent and the cell suspension through the first filter part, separates the impurities in the cell suspension after the reaction reagent and the cell suspension are fully mixed, and delivers the filtered cell suspension to the second filter part through the first accommodation space. The cell suspension delivered by the first filter part is received by the second filter part, and the received cell suspension is further filtered by the filter element before outputting the cell suspension, and the force received by the filter element during filtration is monitored in real time by the detection element, so as to provide a basis for judging whether the filter element is blocked. The blockage of the filter element is cleared by the clearing part when the filter element is blocked, thereby improving the technical problem in the related art that the filtration needs to be stopped and the blocked filter screen needs to be replaced or cleaned manually, which reduces the working efficiency of the cell filtration device.

[0010] In a second aspect, the present application provides a cell filtration method, comprising:

[0011] When the cell filtration device is operating normally and the activity of the filtered cells is greater than the preset activity, filtration information is obtained; wherein the filtration information includes a plurality of groups of filtration data corresponding to the filtration time, each group of filtration data includes two corresponding filtration resistance information transmitted by the detection element, and the filtration resistance information reflects the pressure on the filter element;

[0012] In the case where the filter element is clogged, determining the clogged position of the filter element based on the filtering information;

[0013] The cell filtration device is controlled based on the blockage position.

[0014] The above technical solutions in the embodiments of the present application have at least the following technical effects:

[0015] By first obtaining the filtering information including the filtering resistance information reflecting the pressure on the filter element transmitted by each detection element after the preset startup time under normal operation of the cell filtering device, and when the activity of the filtered cells is greater than the preset activity, a basis is provided for judging whether the blocking element is blocked and the blocked area when the blockage occurs. Then, according to the filtering information, whether the filter element is blocked is analyzed. If the filter element is blocked, the blocked position of the filter element is determined based on the filtering information. Finally, according to the blocked position, the cell filtering device is controlled to clear the blocked area on the filter element, so as to improve the problem of reduced work efficiency due to manual replacement or cleaning of the filter. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0017] Figure 1 A schematic diagram of the structure of a cell filtration device provided in an embodiment of the present application;

[0018] Figure 2 A schematic cross-sectional view of a cell filtration device provided in an embodiment of the present application;

[0019] Figure 3 A schematic diagram of the structure of the filtering mechanism provided in the embodiment of the present application;

[0020] Figure 4 A schematic diagram of the process of the cell filtration method provided in the embodiment of the present application;

[0021] Figure 5 A schematic diagram of the process of step S200 in the cell filtration method provided in an embodiment of the present application;

[0022] Figure 6 This is a schematic flow chart of step S2431 in the cell filtration method provided in an embodiment of the present application.

[0023] Among them, the reference numerals in the figure are:

[0024] 100, cell filtration device; 10, first filter section; 11, first container; 111, first container space; 112, injection hole; 12, second container; 121, rotation container space; 122, primary filter section; 13, rotation drive section; 20, second filter section; 21, storage section; 211, storage element; 212, intercepting element; 213, pressurizing element; 214, second container space; 22, connecting pipe; 221, conveying channel; 23, filtering mechanism; 231, filtering element; 232, detecting element; 233, filtering tube; 234, flow guide element; 235, filtering channel; 236, flow guide hole; 30, clearing section; 31, fixing element; 32, third container; 33, flushing device; 34, image acquisition device; 35, output hole. DETAILED DESCRIPTION

[0025] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present application more clearly understood, the present application is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.

[0026] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs; the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit this application. The terms "including" and "having" and any variations thereof in the specification and claims of this application and the above-mentioned figure descriptions are intended to cover non-exclusive inclusions.

[0027] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

[0028] It should be understood that the terms "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0029] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0030] In this application, "and / or" is only a description of the association relationship of associated objects, indicating that there can be three relationships; for example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. In addition, the character " / " in this article generally indicates that the associated objects before and after are in an "or" relationship.

[0031] It should be noted that, in the present application, words such as "in some embodiments", "exemplarily", "for example", etc. are used to indicate examples, illustrations or descriptions. Any embodiment or design described in the present application as "in some embodiments", "exemplarily", "for example" should not be interpreted as being more preferred or more advantageous than other embodiments or designs. Specifically, the use of words such as "in some embodiments", "exemplarily", "for example", etc. is intended to present related concepts in a concrete way, meaning that specific features, structures or characteristics described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the above words in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0032] Cell filtration devices are instruments used to separate and filter biological molecules or cells. They are widely used in many fields such as cell culture, biopharmaceuticals, biotechnology research, food and beverage industry, and water treatment.

[0033] When the cell filtration device in the related art continuously filters a large amount of cell suspension for a long time, the filter screen is prone to clogging. At this time, it is usually necessary to stop the operation of the cell filtration device and manually replace or clean the filter screen, resulting in reduced work efficiency.

[0034] Based on this, in order to improve the technical problem in the related art that the working efficiency of the cell filtration device is reduced by manually replacing or cleaning the clogged filter, the embodiments of the present application provide the following solution.

[0035] See also Figure 1 The present application embodiment provides a cell filtration device 100, which includes a first filtration unit 10, a second filtration unit 20 and a clearing unit 30, wherein:

[0036] The first filter part 10 has a first containing space 111 . The first filter part 10 is used to receive the reaction reagent and the cell suspension, and separate the impurities in the cell suspension after mixing the reaction reagent and the cell suspension.

[0037] One end of the second filter part 20 is connected to the first filter part 10, and the second filter part 20 has a second accommodating space 214, and the second accommodating space 214 is connected to the first accommodating space 111. The second filter part 20 includes a filter element 231 and a plurality of detection elements 232. The filter element 231 is used to filter the cell suspension, and the detection element 232 is used to obtain the force applied to the filter element 231 during filtering.

[0038] The clearing part 30 is arranged on the other end of the second filter part 20, and the clearing part 30 has a third accommodating space. The other end part of the second filter part 20 is located in the third accommodating space. The third accommodating space is connected to the second accommodating space 214. The clearing part 30 is used to clear the blockage when the filter element 231 is blocked.

[0039] It can be understood that the first filter unit 10 is a device that can receive the cell suspension to be filtered and the reaction reagent to be added, and filter the mixture after mixing the cell suspension and the reaction reagent. For example, the first filter unit 10 can be a filter press, a centrifugal separator, etc., but is not limited thereto.

[0040] The second filter part 20 is a device that can receive the cell suspension delivered by the first filter part 10 and further filter the cell suspension. The second filter part 20 can be arranged below the first filter part 10. For example, the second filter part 20 can include a connecting tube (glass tube, metal tube, etc.) and a filter (nylon filter mesh, stainless steel filter mesh, etc.), but not limited to this. The connecting tube is connected to the first filter part 10, and the filter is arranged in the connecting tube and covers a cross section of the connecting tube. The filter element 231 is a device that can filter impurities or bacteria in the cell suspension. For example, the filter element 231 can be a nylon filter mesh, a stainless steel filter mesh, etc., but not limited to this. The detection element 232 is a device that can obtain the filtration resistance received by the filter element 231 when the filter element 231 is filtering. For example, the detection element 232 can be a pressure sensor, a strain gauge, etc., but not limited to this.

[0041] The clearing unit 30 is a device that can clear the blockage when the filter element 231 is blocked. The clearing unit 30 can be arranged above the output end of the second filter unit 20. For example, the clearing unit 30 can be an ultrasonic cleaning device, a miniature high-pressure water gun, etc., but is not limited to this.

[0042] As can be seen from the above, the cell filter device 100 provided in the embodiment of the present application receives the reaction reagent and the cell suspension through the first filter part 10, and after the reaction reagent and the cell suspension are fully mixed, the impurities in the cell suspension are separated, and the filtered cell suspension is transported to the second filter part 20 through the first accommodation space 111. The second filter part 20 receives the cell suspension transported by the first filter part 10, and outputs the cell suspension after further filtering the received cell suspension through the filter element 231, and monitors the force received by the filter element 231 during filtration in real time through the detection element 232, providing a basis for judging whether the filter element 231 is blocked. When the filter element 231 is blocked, the clearing part 30 clears the blocked part of the filter element 231, thereby improving the technical problem in the related art that the filtering needs to be stopped and the blocked filter screen needs to be replaced or cleaned manually, which reduces the working efficiency of the cell filter device 100.

[0043] In some embodiments, please refer to Figure 1 to Figure 2 The first filter unit 10 includes a first accommodating member 11 , a second accommodating member 12 and a rotation driving member 13 .

[0044] The first container 11 has a first accommodating space 111 . A liquid injection hole 112 communicating with the first accommodating space 111 is formed on a side of the first container 11 away from the second filter portion 20 .

[0045] The second container 12 is rotatably located in the first container space 111 and below the injection hole 112. The second container 12 has a rotational container space 121, which is connected to the first container space 111. The second container 12 has a primary filter portion 122 on the side away from the second filter portion 20, and the primary filter portion 122 is used to separate impurities in the cell suspension.

[0046] The rotary driving part 13 is disposed on a side of the first accommodating member 11 away from the second filtering part 20 . The power output shaft of the rotary driving part 13 is connected to the second accommodating member 12 . The rotary driving part 13 is used to drive the second accommodating member 12 to rotate.

[0047] One end of the second filter portion 20 is connected to the first receiving member 11 .

[0048] It can be understood that the first container 11 is a container capable of accommodating the second container 12. For example, the first container 11 can be an aluminum cylinder, a plastic cylinder, etc., but not limited thereto. The number of injection holes 112 can be one or more, but not limited thereto. The second container 12 is a barrel-shaped structure with an opening on one side close to the injection hole 112. For example, the second container 12 can be an aluminum barrel, a plastic barrel, etc., but not limited thereto. The primary filter 122 is a filtering device installed on the side wall of the second container 12 close to the injection hole 112. The primary filter 122 has an annular protrusion extending horizontally toward the rotation drive unit 13 on the side away from the second filter 20. For example, the primary filter 122 can be a cell filter, a stainless steel cell screen, etc., but not limited thereto. The rotation drive unit 13 is a driving device capable of driving the second container 12 to rotate. For example, the rotation drive unit 13 can be a stepping motor, a servo motor, etc., but not limited thereto.

[0049] With such a configuration, when the cell suspension needs to be filtered, the rotation driving unit 13 first drives the second container 12 to rotate, so that the second container 12 reaches a preset rotation speed, and then receives the cell suspension to be filtered and the reaction reagent to be added through the injection hole 112 opened on the first container 11 and connected to the first container space 111, so that the cell suspension and the reaction reagent pass through the first container space 111 along the gravity direction and enter the rotating container space 121, and the cell suspension and the reaction reagent are fully mixed by the rotation of the second container 12. By controlling the rotation speed of the second container 12, the cell suspension at the bottom of the second container 12 that is fully mixed with the reaction reagent rises along the side wall of the second container 12 to the height of the primary filter 122 under the action of centrifugal force, but does not exceed the height of the primary filter 122, so that the cell suspension passes through the primary filter 122 under the action of centrifugal force, and flows from the first container space 111 between the first container 11 and the second container 12 to the second filter 20, thereby separating impurities in the cell suspension. When the cell suspension is filtered through the primary filter 122 , the annular protrusion on the primary filter 122 prevents the cell suspension from directly flowing out of the opening on the side of the second container 12 close to the injection hole 112 due to an excessively fast rotation speed.

[0050] Illustratively, during the filtering of the cell suspension through the primary filter section 122, the rotation speed of the second container 12 can be increased or decreased to achieve wave-like fluctuations in height of the cell suspension, thereby flushing out impurities that may clog the primary filter section 122 and preventing impurities from clogging the primary filter section 122 and affecting the filtration efficiency.

[0051] In some embodiments, see Figure 1 to Figure 2 The second filter unit 20 includes a storage unit 21 , a connecting pipe 22 and a filter mechanism 23 .

[0052] One end of the storage part 21 is connected to the first filter part 10 , and the storage part 21 has a second accommodation space 214 .

[0053] The input end of the connecting pipe 22 is connected to the other end of the storage part 21 . The connecting pipe 22 has a conveying channel 221 . The conveying channel 221 is connected to the second accommodation space 214 .

[0054] The filtering mechanism 23 is located in the third containing space and is detachably disposed on the other end of the connecting tube 22 . The filtering mechanism 23 has a filtering channel 235 , which is connected to the conveying channel 221 . The filtering mechanism 23 is used to further filter the cell suspension conveyed from the output end of the connecting tube 22 .

[0055] The clearing portion 30 is disposed on the output end of the connecting pipe 22 , and the third accommodating space is connected to the second accommodating space 214 through the conveying channel 221 .

[0056] It can be understood that the storage part 21 is a barrel-shaped structure that can make the first storage space 111 and the second storage space 214 disconnected. The storage part 21 can be located below the first filter part 10. For example, the storage part 21 can include a storage tank (aluminum tank, glass tank, etc.) and a shut-off valve, etc., but is not limited to this. The connecting pipe 22 is a U-shaped tube. The connecting pipe 22 can be located below the filter mechanism 23 and the storage part 21. For example, the connecting pipe 22 can be a glass U-shaped tube, a plastic U-shaped tube, etc., but is not limited to this. The filter mechanism 23 is a filtering device that can filter the cell suspension. For example, the filter mechanism 23 can be a nylon filter mesh, a stainless steel filter mesh, etc., but is not limited to this.

[0057] In this way, the cell suspension is guided to flow into the connecting tube 22 through the storage part 21, and when the filter channel 235 in the connecting tube 22 is full of the cell suspension, the second storage space 214 provides additional storage space. When the filter mechanism 23 needs to be replaced, the cell suspension output by the first filter part 10 is prevented from flowing into the connecting tube 22 by making the first storage space 111 and the second storage space 214 disconnected. By setting the connecting tube 22 as a U-shaped tube and making the connecting tube 22 located below the filter mechanism 23 and the storage part 21, when the cell suspension flows from bottom to top through the connecting tube 22 to the filter mechanism 23, the impurities in the cell suspension are close to the bottom of the connecting tube 22 under the action of gravity, making it more difficult for the impurities in the cell suspension to approach the filter mechanism 23, thereby reducing the possibility of the filter mechanism 23 being blocked. By detachably setting the filter mechanism 23 on the other end of the connecting tube 22, when the filter mechanism 23 is seriously blocked, the filter mechanism 23 can be quickly replaced to reduce the impact on the filtration efficiency.

[0058] In some embodiments, see Figure 1 to Figure 2 The storage portion 21 includes a storage element 211 , a shut-off element 212 and a pressurizing element 213 .

[0059] One end of the storage element 211 is connected to the first filter portion 10 , and the storage element 211 has a second accommodating space 214 .

[0060] The shutoff member 212 is disposed on a side of the storage member 211 close to the first filter portion 10 , and the shutoff member 212 is used to prevent the second accommodating space 214 from being communicated with the first accommodating space 111 .

[0061] The pressurizing element 213 is disposed on the storage element 211 , and is used to increase the pressure in the second accommodation space 214 .

[0062] The connecting pipe 22 is connected to the other end of the storage element 211 , and the height of the output end of the connecting pipe 22 away from the ground is equal to or higher than the height of the intercepting element 212 close to the ground.

[0063] It can be understood that the storage member 211 is a barrel-shaped structure. For example, the storage member 211 can be an aluminum can, a glass can, etc., but it is not limited thereto. The shut-off member 212 is a device that can disconnect the second accommodation space 214 from the first accommodation space 111. For example, the shut-off member 212 can be a shut-off valve, a gate valve, etc., but it is not limited thereto. The pressurizing member 213 is a pressurizing device that can increase the pressure in the second accommodation space 214 when the shut-off member 212 is in a closed state. For example, the pressurizing member 213 can be an air supercharger, a gas supercharger pump, etc., but it is not limited thereto.

[0064] With such arrangement, when it is necessary to stop the operation of the cell filtration device 100, the intercepting member 212 is switched from the open state to the closed state, so that the second accommodation space 214 is disconnected from the first accommodation space 111, and then the pressurizing member 213 increases the pressure in the second accommodation space 214, and the residual cell suspension in the connecting tube 22 flows to the filtering mechanism 23 under the action of pressure, thereby emptying the residual cell suspension in the connecting tube 22. When it is necessary to suspend the filtering of the cell suspension, the intercepting member 212 is switched from the open state to the closed state, so that the second accommodation space 214 is disconnected from the first accommodation space 111. Since the height of the side of the output end of the connecting tube 22 away from the ground is equal to or higher than the height of the side of the intercepting member 212 close to the ground, the residual cell suspension in the connecting tube 22 will not continue to flow to the filtering mechanism 23. Only when the first filtering part 10 continues to output the cell suspension, the cell suspension in the connecting tube 22 will continue to flow to the filtering mechanism 23.

[0065] Illustratively, during the process of filtering the cell suspension, the pressurizing member 213 can also increase the pressure in the second accommodation space 214 so that the cell suspension in the connecting tube 22 flows toward the filtering mechanism 23 faster under the action of pressure, thereby improving the filtering rate.

[0066] In some embodiments, see Figures 1 to 3 The filtering mechanism 23 also includes a filtering tube 233 and a flow guide 234 .

[0067] The filter tube 233 is located in the third accommodating space and is detachably disposed on the other end of the connecting tube 22 . The filter tube 233 has a filter channel 235 . A flow guide hole 236 communicating with the filter channel 235 is formed on the side wall of the filter tube 233 .

[0068] The guide member 234 is partially located in the third accommodating space and between the guide hole 236 and the input end of the filter tube 233, and is arranged on the filter tube 233 around the side wall of the filter tube 233. The guide member 234 is used to receive the cell suspension output by the guide hole 236 and output the cell suspension.

[0069] Among them, the filter element 231 is located in the filter channel 235 and below the guide hole 236, and is arranged on the side of the filter tube 233 close to the connecting tube 22. The filter element 231 is used to filter the cell suspension transported from the output end of the connecting tube 22; the detection element 232 is located in the filter channel 235 and connected to the filter element 231, and is arranged on the filter tube 233. The detection element 232 is evenly distributed along the circumference of the filter element 231, and at least two filters 231 correspond to each other along the diameter direction of the filter element 231.

[0070] It can be understood that the filter tube 233 is a tubular structure, for example, the filter tube 233 can be a glass tube, a plastic tube, etc., but not limited thereto. The flow guide 234 is a disc-shaped structure, which can be tilted on the filter tube 233, for example, the flow guide 234 can be a glass disc, a plastic disc, etc., but not limited thereto.

[0071] With such arrangement, when filtering the cell suspension, the cell suspension transported by the connecting tube 22 to the filter channel 235 can be directly filtered by the filter element 231, and the cell suspension filtered by the filter element 231 flows to the guide element 234 through the guide hole 236 provided on the filter tube 233, and the flow direction of the cell suspension is guided by the guide element 234 and the cell suspension is output. By arranging the filter element 231 on the filter tube 233 detachably arranged on the connecting tube 22, rather than arranging the filter element 231 directly on the output end of the connecting tube 22, it is convenient for the user to replace the filter element 231 when the filter element 231 is seriously blocked. By evenly distributing multiple detection elements 232 along the circumference of the filter element 231, and at least two filter elements 231 corresponding to each other along the diameter direction of the filter element 231, the filter element 231 can improve the accuracy of obtaining the force condition and optimize the force balance when filtering the cell suspension.

[0072] In some embodiments, see Figures 1 to 3 The clearing portion 30 includes a fixing member 31 , a third receiving member 32 , a flushing device 33 and an image acquisition device 34 .

[0073] The fixing member 31 is disposed on one end of the connecting pipe 22 close to the filtering mechanism 23 .

[0074] The third accommodating member 32 is detachably disposed on the fixing member 31, and has a third accommodating space. An output hole 35 connected to the third accommodating space is provided on one side of the third accommodating member 32 close to the fixing member 31, and the guide member 234 is partially located outside the third accommodating space through the output hole 35.

[0075] The flushing device 33 is located in the third accommodating space and is rotatably disposed on a side of the third accommodating member 32 away from the fixing member 31 . The flushing device 33 is used for flushing the blocked area when the filter member 231 is blocked.

[0076] The image acquisition device 34 is located in the third accommodating space and is disposed on a side of the third accommodating member 32 away from the fixing member 31 . The image acquisition device 34 is used to acquire an image of the filter 231 .

[0077] It can be understood that the fixing member 31 is a plate-like structure, for example, the fixing member 31 can be a plastic plate, an aluminum plate, etc., but not limited thereto. The third container 32 is a barrel-like structure, and the connection between the third container 32 and the fixing member 31 can be a snap connection or a hinge connection, etc., but not limited thereto. For example, the third container 32 can be a plastic barrel, an aluminum barrel, etc., but not limited thereto. The flushing device 33 is a device capable of flushing the filter 231, for example, the flushing device 33 can be an electric micro water gun, a high-pressure micro water gun, etc., but not limited thereto. The image acquisition device 34 is a device capable of capturing an image of the filter 231, for example, the image acquisition device 34 can be a charge coupled device (CCD) camera, an industrial camera, etc., but not limited thereto.

[0078] In this way, by placing the filter mechanism 23 in the third storage space, the possibility of the cell suspension output by the filter mechanism 23 being contaminated can be reduced. When the filter mechanism 23 needs to be replaced, the third storage member 32 is separated from the fixing member 31. When the filter mechanism 23 is replaced, the third storage member 32 is combined with the fixing member 31 so that the filter mechanism 23 is located in the third storage space. When the filter 231 is clogged, the clogged part of the filter 231 can be flushed by the flushing device 33, and the photos of the filter 231 before and after flushing can be obtained by the image acquisition device 34 to determine whether the filter 231 is unblocked.

[0079] See also Figures 4 to 6 The present application also provides a cell filtration method, which comprises:

[0080] When the cell filtration device 100 is operating normally and after a preset start-up time, and the activity of the filtered cells is greater than a preset activity, filtration information is obtained; wherein the filtration information includes multiple groups of filtration data corresponding to the filtration time, each group of filtration data includes two corresponding filtration resistance information transmitted by detection elements 232, and the filtration resistance information reflects the pressure on the filter element 231.

[0081] When the filter element 231 is clogged, the clogged position of the filter element 231 is determined based on the filtering information.

[0082] The blockage clearing unit 30 is controlled based on the blockage position.

[0083] As can be seen from the above, the cell filtering method provided in the embodiment of the present application provides a basis for judging whether the blocking element is blocked and the blocked area when the blocking occurs by first obtaining the filtering information including the filtering resistance information reflecting the pressure on the filter element 231 transmitted by each detection element 232 after the preset startup time under the condition of normal operation of the cell filtering device 100 and when the activity of the filtered cells is greater than the preset activity. Then, the filtering information is analyzed based on the filtering information to determine whether the filter element 231 is blocked. When the filter element 231 is blocked, the blocking position of the filter element 231 is determined based on the filtering information. Finally, the clearing unit 30 is controlled to clear the blocked area on the filter element 231 according to the blocking position, so as to improve the problem of reduced work efficiency due to manual replacement or cleaning of the filter net.

[0084] In order to better understand the cell filtration method provided in the embodiments of the present application, the specific implementation process of the cell filtration method provided in the embodiments of the present application is exemplarily introduced below.

[0085] Figure 4 A schematic flow chart of a cell filtration method provided in an embodiment of the present application is shown, and the cell filtration method comprises:

[0086] S100, after the preset start-up time has passed under the normal operation of the cell filtration device 100, and when the activity of the filtered cells is greater than the preset activity, the filtration information is obtained; wherein the filtration information includes multiple groups of filtration data corresponding to the filtration time, each group of filtration data includes two corresponding filtration resistance information transmitted by the detection elements 232, and the filtration resistance information reflects the pressure on the filter element 231.

[0087] It can be understood that the preset start time can be 30 seconds, or 1 minute, etc., but not limited thereto. The preset activity can be a percentage defined by the user, or 80 percent, etc., but not limited thereto. The method for judging that the activity of the filtered cells is greater than the preset activity can be to receive the data returned by the user after analyzing the cell activity in the filtered cell suspension, or to receive the picture taken by the cell filtration device 100, and input the picture into the cell image processing and analysis software (CellProfiler, FlowJo, etc.), and then receive the analysis data of the cell activity of the software, etc., but not limited thereto. When the filtration resistance information corresponds to only one time node, the expression form of the filtration resistance information is a force value, and when the filtration resistance information corresponds to a time period, the expression form of the filtration resistance information is a continuous line segment with each time node in the time period as an inflection point. The two corresponding detection members 232 are the two detection members 232 at both ends of the diameter of the filter member 231. Obtaining the filtration information including multiple groups of filtration data corresponding to the filtration time can provide a basis for subsequent steps.

[0088] S200 , when the filter element 231 is clogged, determining the clogged position of the filter element 231 based on the filtering information.

[0089] It can be understood that the method for determining whether the filter element 231 is blocked may be to determine whether the pressure on the filter element 231 reflected by the obtained filtration resistance information is greater than the preset pressure, or to determine whether the average value of the pressure on the filter element 231 reflected by the filtration resistance information obtained within a time period is greater than the preset pressure, etc., but not limited thereto. The method for determining the blocked position of the filter element 231 based on the filtration information may be to input the image taken by the cell filtration device 100 into the image analysis model to obtain an analysis image reflecting the blocked position of the filter element 231, or to obtain the blocked area based on multiple sets of filtration data reflecting the forces received by the filter element 231 in various diameter directions, etc., but not limited thereto. Determining the blocked position of the filter element 231 based on the filtration information can ensure the accuracy and reliability of the blocked position and provide a basis for clearing the blocked position of the filter element 231.

[0090] In one possible implementation, see Figure 5 , determining the blockage position of the filter element 231 based on the filtering information, including:

[0091] S210, real-time monitoring of the filtration resistance information. When any filtration resistance information reflects a filtration resistance greater than the preset resistance, the time node corresponding to the filtration resistance information where the filtration resistance is greater than the preset resistance is confirmed as a warning node, and the time period after the preset warning time with the warning node as the starting point is confirmed as the warning time period.

[0092] It is understood that the preset resistance may be a value defined by the user, or may be 1 Newton, etc., but is not limited thereto. The preset warning time may be a value defined by the user, or may be 10 seconds, etc., but is not limited thereto. Confirming the warning time can provide a basis for subsequent steps.

[0093] S220: Confirm the warning time period and the filtering resistance information included in each group of filtering data within the warning time period as analysis information.

[0094] It can be understood that confirming the filter resistance information included in the warning time period and each group of filter data within the warning time period as analysis information can provide more comprehensive data for analyzing the blockage of the filter element 231, avoid contingency, enhance the reliability of the analysis results, and provide a basis for subsequent steps.

[0095] S230, respectively calculating the average values ​​of the filtration resistances reflected by each filtration resistance information within the warning time period, and obtaining a plurality of warning average values ​​corresponding to each filtration resistance information within the warning time period.

[0096] It can be understood that the way to calculate the average value of the filtration resistance reflected by each filtration resistance information in the warning time period to obtain the warning mean value can be to add the pressure values ​​corresponding to each filtration resistance information and each time node in the warning time period and then divide by the number of time nodes in the warning time period, or to send data to the user and receive data sent back by the user, but it is not limited to this. Obtaining multiple warning mean values ​​corresponding to each filtration resistance information in the warning time period can provide a basis for the subsequent process.

[0097] Exemplarily, assuming that the warning time period is 3 seconds, the number of time nodes is 3, the interval between each time node is 1 second, there are 2 filter resistance information in the warning time period, the 2 filter resistance information are numbered A and B respectively, and the relationship between the 2 filter resistance information and the warning time period is shown in Table 1 below. Then, the warning mean of the filter resistance information numbered A = (1+1.1+0.9) / 3 = 1N, and the warning mean of the filter resistance information numbered B = (1+1.2+0.8) / 3 = 1N.

[0098] Table 1:

[0099]

[0100] S240, determine whether the filtration resistance reflected by each warning mean is within the preset resistance range. If the filtration resistance reflected by the warning mean is not within the preset resistance range, the blockage position is obtained based on the analysis information. If the filtration resistance reflected by each warning mean is within the preset resistance range, an operation instruction is obtained to instruct the cell filtration device 100 to continue operating; wherein the preset resistance range is a numerical range with a fluctuation of 5% above and below the preset resistance.

[0101] It can be understood that the method of obtaining the blockage location based on the analysis information can be to send the analysis information to the user and then receive the data sent back by the user, or it can be obtained based on the force value reflected by the filtering resistance information corresponding to each time node in the analysis information, etc., but is not limited to this.

[0102] For example, assuming that the preset resistance is 1 Newton, the preset resistance range is (1*1.05, 1*0.95)=(1.05, 0.95).

[0103] In one possible implementation, see Figure 5 In step S240, the blocking location is obtained based on the analysis information, including:

[0104] S241, obtaining a filter surface image; wherein the filter surface image includes a cross-sectional image and a plurality of detection points marked on the cross-sectional image, the cross-sectional image is a filter surface image of the filter element 231, the positions of the detection points on the cross-sectional image reflect the relative positions of each detection element 232 and the filter element 231, and each detection point corresponds to a filter resistance information.

[0105] It is understood that the method of acquiring the filter surface image may be to receive the image data reflecting the filter surface of the filter element 231 transmitted by the user, or to mark the area reflecting the detection element 232 on the image data as a detection point after receiving the image data transmitted by the image acquisition device 34, but is not limited thereto. Acquiring the filter surface image can provide a basis for subsequent steps.

[0106] S242, confirming each group of filtering data corresponding to each time node in the warning time period as single-layer information.

[0107] It can be understood that by confirming each group of filtering data corresponding to each time node in the warning time period as a single layer of information, the stress condition of the filter element 231 can be analyzed separately based on each time node, thereby improving the accuracy of the analysis result.

[0108] S243, obtaining the blockage position based on all the single-layer information and the filter surface image.

[0109] It can be understood that the method of obtaining the blockage position based on all the single-layer information and the filter surface image can be to perform force analysis on each group of filter data in the single-layer information to obtain the blockage position marked on the filter surface image, or to send the single-layer information and the filter surface image to the user and then receive the data sent back by the user, etc., but it is not limited thereto. Obtaining the blockage position based on all the single-layer information and the filter surface image can ensure the reliability and accuracy of the obtained blockage position.

[0110] In one possible implementation, see Figure 5 , S243, obtaining the blockage position based on all the single-layer information and the filter surface image, including:

[0111] S2431, obtaining a plurality of single-layer analysis information based on each single-layer information; wherein the single-layer analysis information includes a plurality of blockage point information, and each blockage point information corresponds to a set of filtering data.

[0112] It can be understood that a blockage point information reflects the stress condition of the filter element 231 on a diameter. The method of obtaining multiple single-layer analysis information based on single-layer information can be to perform force analysis on two filter resistance information corresponding to each group of filter data in the single-layer information to obtain at least one stress point, and then confirm each stress point as blockage point information, or to send each single-layer information to the user and then receive data sent back by the user, etc., but it is not limited to this. Obtaining multiple single-layer analysis information based on each single-layer information can provide a basis for subsequent steps.

[0113] In one possible implementation, see Figure 6 , S2431, obtaining a plurality of single-layer analysis information based on each single-layer information, including:

[0114] S24311, respectively perform numerical comparison on the filtration resistances reflected by the two filtration resistance information in each group of filtration data in the single-layer information, confirm the detection point corresponding to the detection element 232 corresponding to the filtration resistance information whose filtration resistance is smaller than the other filtration resistance as the starting point, and confirm the detection point corresponding to the detection element 232 corresponding to the filtration resistance information whose filtration resistance is larger than the other filtration resistance as the extension point.

[0115] It can be understood that, assuming that the filter resistance reflected by one filter resistance information is numbered A, and the filter resistance reflected by the other filter resistance information is numbered B, and the filter resistance numbered A is greater than the filter resistance numbered B, it means that on the straight line connecting the detection members 232 corresponding to the two filter resistance information, the force point is closer to the detection member 232 corresponding to the filter resistance information numbered A. Confirming the detection point corresponding to the detection member 232 corresponding to the filter resistance information with a filter resistance smaller than the other filter resistance as the starting point, and confirming the detection point corresponding to the detection member 232 corresponding to the filter resistance information with a filter resistance greater than the other filter resistance as the extension point can provide a basis for subsequent steps.

[0116] Exemplarily, assuming that the two filter resistance information in the filter data reflect filter resistances of 1 Newton and 1.5 Newton respectively, the detection point corresponding to the filter resistance information of 1 Newton is the starting point, and the detection point corresponding to the filter resistance information of 1.5 Newton is the extension point.

[0117] S24312, the direction of the straight line extending from the starting point to the extension point is confirmed as the extension direction, the diameter of the cross-sectional image is divided by the sum of the filtration resistances reflected by the two filtration resistance information to obtain the extension ratio, and the absolute value of the difference between the filtration resistances reflected by the two filtration resistance information is confirmed as the extension multiple, and the extension ratio is multiplied by the extension multiple to obtain the extension distance.

[0118] It can be understood that confirming the extension direction and extension distance can provide a basis for subsequent steps.

[0119] For example, assuming that the diameter of the cross-sectional image is 5 cm, the two filtration resistance information reflect filtration resistances of 1 N and 1.5 N respectively, the extension ratio = 5 / (1+1.5) = 2, the extension multiple = |1-1.5| = 0.5 cm, and the extension distance = 2*0.5 = 1 cm.

[0120] S24313, confirming the extension distance and the extension direction as the blocking point information; wherein the blocking point information corresponds to a set of filtering data.

[0121] It can be understood that confirming the extension distance and extension direction as the blocking point information can provide a basis for subsequent steps.

[0122] S24314, confirm all the blockage point information as single-layer analysis information.

[0123] It can be understood that confirming all the blockage point information as single-layer analysis information can provide a basis for subsequent steps.

[0124] S2432, obtaining the blockage location based on all single-layer analysis information and the filter surface image.

[0125] It can be understood that the method of obtaining the blockage position based on all the single-layer analysis information and the filter surface image can be to take the center of the filter surface image as the starting point, obtain multiple extension line segments based on various extension directions and corresponding extension distances, confirm the endpoints of each extension line segment away from the center of the circle as the blockage point, and then confirm the closed area formed by connecting the various blockage points as the blockage position, or send all the single-layer analysis information and the filter surface image to the user and receive the data sent back by the user, etc., but it is not limited to this. Obtaining the blockage position based on all the single-layer analysis information and the filter surface image can ensure the reliability of the blockage position.

[0126] In one possible implementation, see Figure 6 , S2432, obtaining the blockage location based on all the single layer analysis information and the filter surface image, including:

[0127] S24321, obtaining the blockage range information based on all the single-layer analysis information and the filter surface image; wherein the blockage range information includes a plurality of blockage surfaces, each blockage surface corresponds to a single-layer analysis information, and the blockage surface includes the filter surface image and the blockage area marked on the filter surface image.

[0128] It can be understood that the method of obtaining the blocking range information based on all the single-layer analysis information and the filter surface image can be to obtain multiple blocking points on the filter surface image based on the extension direction and extension distance in each single-layer analysis information, and to confirm the closed area formed by connecting each adjacent blocking point with a straight line as the blocking range information, or to send all the single-layer analysis information and the filter surface image to the user and then receive the data sent back by the user, etc., but it is not limited to this. Obtaining the blocking range information based on all the single-layer analysis information and the filter surface image can ensure the reliability of the blocking range information and provide a basis for subsequent steps.

[0129] In one possible implementation, see Figure 6 , S24321, based on all single-layer analysis information and filter surface images, obtain blockage range information, including:

[0130] S243211, analyze each single layer analysis information respectively, take the center of the filter surface image as the starting point, extend along the extension direction of each blocking point information in the single layer analysis information respectively by the extension distance, and confirm the endpoint of each extension line as the blocking point.

[0131] It can be understood that identifying the endpoint of each extension line away from the center of the circle as a blocking point can provide a basis for subsequent steps.

[0132] S243212, after connecting each adjacent blocking point along a straight line, the formed closed figure is confirmed as a blocking area, and the blocking area and the filter surface image are confirmed as a blocking surface.

[0133] It can be understood that the method of determining adjacent blocking points can be to use the blocking point as the center and draw a circle with a gradually increasing radius. When the drawn circle contacts another blocking point for the first time, the blocking point that contacts for the first time is adjacent to the blocking point at the center of the circle. Alternatively, the straight-line distance between the blocking point and each other blocking point can be calculated, and the blocking point with the shortest straight-line distance can be identified as an adjacent blocking point, etc., but it is not limited thereto. Identifying the blocked area and the filter surface image as the blocked surface can provide a basis for subsequent steps.

[0134] S243213, after completing the analysis of all single-layer analysis information, all blocked surfaces are confirmed as blocked range information.

[0135] It can be understood that confirming all congestion surfaces as congestion range information can ensure the reliability of the congestion range information.

[0136] S24322: After stacking the filter surface images in the blocking range information along the same direction, the overlapping portion of the blocking areas in the filter surface images is identified as the blocking position.

[0137] It can be understood that the way to stack the filter images in the blocking range information in the same direction may be to stack the filter images in the same direction and overlap the edges of the filter images, or to mark multiple feature points on the filter images and overlap the corresponding feature points on the filter images. Compared with only confirming the blocking area in one filter image as the blocking position, confirming the overlapping part of the blocking area in each filter image as the blocking position can be more accurate and improve the reliability of the blocking position.

[0138] S300, controlling the cell filtration device 100 based on the blockage position.

[0139] It is understood that the method of controlling the cell filter device 100 based on the blockage position may be to control the flushing device 33 to flush the position reflecting the blockage position on the filter element 231, or to control the intercepting member 212 to be closed and then control the flushing device 33 to flush the position reflecting the blockage position on the filter element 231, etc., but is not limited thereto. Controlling the cell filter device 100 based on the blockage position can improve the technical problem in the related art that the working efficiency of the cell filter device 100 is reduced by manually replacing or cleaning the blocked filter screen.

[0140] In a possible implementation, S300, controlling the cell filtration device 100 based on the blockage position, includes:

[0141] S310, issuing a first control instruction to the flushing device 33; wherein the first control instruction is used to instruct the flushing device 33 to rotate and make the flushing direction of the flushing device 33 intersect with the position where the filter element 231 is clogged as reflected by the filtration analysis information.

[0142] It can be understood that the first control instruction for instructing the flushing device 33 to rotate can be issued to the flushing device 33 by controlling the flushing device 33 to start from the center of the blocked area after the fuzzy processing and move away from the blocked area in a circular path with a gradually increasing radius after the fuzzy processing of the blocked area, or by controlling the flushing device 33 to start from the center of the blocked area after the fuzzy processing and move back and forth along the radial direction of the filter element 231 after the fuzzy processing of the blocked area. By issuing the first control instruction for instructing the flushing device 33 to rotate and making the flushing direction of the flushing device 33 intersect with the position where the filter analysis information reflects that the filter element 231 is blocked, the flushing device 33 can be prevented from flushing other non-blocked positions of the filter element 231, thereby saving water resources and increasing the service life of the filter element 231.

[0143] S320, issuing a second control instruction to the flushing device 33; wherein the second control instruction is used to instruct the flushing device 33 to flush the location where the filter element 231 is clogged as reflected in the filtration analysis information with a preset flushing force.

[0144] It is understood that the preset flushing force can be a numerical force defined by the user, or can be 1 Newton, etc., but is not limited thereto. By sending a second control instruction to the flushing device 33 to instruct the flushing device 33 to flush the position where the filter element 231 is clogged as reflected by the filtration analysis information, the clogging of the filter element 231 can be improved without stopping the cell filtration, thereby improving the technical problem in the related art that the work efficiency of the cell filtration device 100 is reduced by manually replacing or cleaning the clogged filter.

[0145] Exemplarily, before issuing the second control instruction, a first image reflecting the blockage condition of the filter surface of the filter element 231 can also be obtained through the image acquisition device 34. After issuing the second control instruction and the flushing device 33 is flushed, a second image reflecting the blockage condition of the filter surface of the filter element 231 is obtained through the image acquisition device 34. It can be determined by performing image analysis processing on the first image and the second image to determine whether the blocked position of the filter element 231 is unblocked after the flushing device 33 is flushed. Alternatively, the first image and the second image can be sent to the user, who can determine whether the blocked position of the filter element 231 is unblocked and whether the filter element 231 needs to be replaced.

[0146] Illustratively, when the second control instruction is issued, the filtration resistance information obtained by the detection element 232 during the flushing process of the flushing device 33 and within 10 seconds or 20 seconds after the flushing of the flushing device 33 is completed can be marked as invalid data. The filtration resistance information marked as invalid data will not participate in the cell filtration method in any of the above embodiments, thereby ensuring the reliability of the method.

[0147] The present application also provides a cell filtration device 100 in an embodiment. The cell filtration device 100 in this embodiment includes: a first filter part 10, a second filter part 20, a clearing part 30 and a control terminal, wherein the control terminal is respectively communicated with the second filter part 20 and the clearing part 30, and the control terminal includes: at least one processor, at least one memory and a computer program stored in the at least one memory and executable on the at least one processor. When the processor executes the computer program, the cell filtration device 100 implements the steps in any of the above-mentioned cell filtration method embodiments.

[0148] Exemplarily, the computer program may be divided into one or more modules / units, which are stored in the memory and executed by the processor to complete the present application. The one or more modules / units may be a series of computer program instruction segments capable of completing specific functions, which are used to describe the execution process of the computer program in the control terminal.

[0149] The control terminal can be a computing device such as a programmable logic controller (PLC), an industrial control computer (IPC), a single-chip microcomputer, a desktop computer, a notebook, a handheld computer, and a cloud server. The control terminal may include, but is not limited to, a processor and a memory. Those skilled in the art will appreciate that the above embodiments are merely examples of the cell filtration device 100 and do not constitute a limitation on the cell filtration device 100, which may include more or fewer components, or a combination of certain components, or different components, such as input and output devices, network access devices, buses, etc.

[0150] The processor may be a central processing unit (CPU), or other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field-programmable gate arrays (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. A general-purpose processor may be a microprocessor or the processor may be any conventional processor, etc.

[0151] In some embodiments, the memory may be an internal storage unit of the control terminal, such as a hard disk or memory of the control terminal. In other embodiments, the memory may also be an external storage device of the control terminal, such as a plug-in hard disk, a smart media card (SMC), a secure digital (SD) card, a flash card, etc. equipped on the control terminal. Furthermore, the memory may include both an internal storage unit and an external storage device of the control terminal. The memory is used to store an operating system, an application program, a boot loader (BootLoader), data, and other programs, such as the program code of the computer program. The memory may also be used to temporarily store data that has been output or is to be output.

[0152] An embodiment of the present application further provides a computer-readable storage medium, wherein the computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, the steps in any of the above method embodiments are implemented.

[0153] An embodiment of the present application provides a computer program product. When the computer program product is executed on a control terminal, the control terminal implements the steps in any of the above method embodiments.

[0154] If the integrated unit is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a computer-readable storage medium. Based on this understanding, the present application implements all or part of the processes in the above-mentioned embodiment method, which can be completed by instructing the relevant hardware through a computer program, and the computer program can be stored in a computer-readable storage medium. When the computer program is executed by the processor, the steps of the above-mentioned method embodiments can be implemented. Among them, the computer program includes computer program code, and the computer program code can be in source code form, object code form, executable file or some intermediate form. The computer-readable medium may at least include: any entity or device that can carry the computer program code to the control terminal, a recording medium, a computer memory, a read-only memory (ROM, Read-Only Memory), a random access memory (RAM, Random Access Memory), an electric carrier signal, a telecommunication signal, and a software distribution medium. For example, a USB flash drive, a mobile hard disk, a disk or an optical disk.

[0155] In the above embodiments, the description of each embodiment has its own emphasis. For parts that are not described or recorded in detail in a certain embodiment, reference can be made to the relevant descriptions of other embodiments.

[0156] Those of ordinary skill in the art will appreciate that the units and algorithm steps of each example described in conjunction with the embodiments disclosed herein can be implemented in electronic hardware, or a combination of computer software and electronic hardware. Whether these functions are performed in hardware or software depends on the specific application and design constraints of the technical solution. Professional and technical personnel can use different methods to implement the described functions for each specific application, but such implementation should not be considered to be beyond the scope of this application.

[0157] In the embodiments provided in the present application, it should be understood that the disclosed cell filtration device and the filtration method thereof can be implemented in other ways. For example, the cell filtration device and the filtration method thereof described above are merely illustrative. In addition, the coupling or direct coupling or communication connection shown or discussed can be an indirect coupling or communication connection through some interface, device or unit, which can be electrical, mechanical or other forms.

[0158] The units described as separate components may or may not be physically separated, and the components shown as units may or may not be physical units, that is, they may be located in one place or distributed on multiple network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0159] The above description is only a preferred embodiment of the present application and is not intended to limit the present application. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. A cell filtration method, characterized in that: Applicable to a cell filtration device, the cell filtration device comprising: A first filter portion having a first accommodating space; a second filter portion having a second accommodating space, one end of the second filter portion being connected to the first filter portion, the second accommodating space being communicated with the first accommodating space, and the second filter portion comprising a filter element and a plurality of detection elements; and The clearing and plugging part has a third accommodating space, the clearing and plugging part is arranged on the other end of the second filter part, the other end of the second filter part is partially located in the third accommodating space, and the third accommodating space is connected to the second accommodating space; The first filter unit comprises: The first container has a first accommodating space, and a liquid injection hole communicating with the first accommodating space is provided on a side of the first container away from the second filter portion; A second container has a rotational accommodating space, the rotational accommodating space is connected to the first accommodating space, and the second container has a primary filtration part on a side away from the second filtration part, the primary filtration part is used to separate impurities in the cell suspension; and Rotary drive unit; The primary filter part surrounds and is arranged on the side wall of the second container close to the injection hole, and the primary filter part has an annular protrusion extending toward the rotary drive part in the horizontal direction on a side away from the second filter part; The method comprises: When the cell filtering device is operating normally and a preset start-up time has passed, and the activity of the filtered cells is greater than a preset activity, obtaining filtering information; In the case of clogging of the filter element, the clogging position of the filter element is determined based on the filtering information: taking the center of the filter surface image as the starting point, a plurality of extended line segments are obtained based on various extension directions and corresponding extension distances, and after the end points of each extended line segment away from the center of the circle are confirmed as clogging points, the closed area formed by connecting the various clogging points is confirmed as the clogging position; The cell filtration device is controlled based on the blockage position.

2. The cell filtration method according to claim 1, characterized in that The rotary drive unit is arranged on a side of the first container away from the second filter unit, the power output shaft of the rotary drive unit is connected to the second container, and the rotary drive unit is used to drive the second container to rotate; one end of the second filter unit is connected to the first container.

3. The cell filtration method according to claim 1, characterized in that The second filter unit comprises: a storage part, one end of which is connected to the first filter part, and the storage part has the second accommodation space; a connecting pipe, the input end of which is connected to the other end of the storage unit, the connecting pipe having a conveying channel, the conveying channel being connected to the second accommodation space; and a filtering mechanism, located in the third containing space and detachably disposed on the other end of the connecting tube, the filtering mechanism having a filtering channel, the filtering channel being connected to the conveying channel, and the filtering mechanism being used to further filter the cell suspension conveyed from the output end of the connecting tube; Wherein, the clearing portion is arranged on the output end of the connecting pipe, and the third accommodating space is connected to the second accommodating space through the conveying channel.

4. The cell filtration method according to claim 3, characterized in that The storage unit includes: A storage element, one end of which is connected to the first filter portion, and the storage element has the second accommodation space; a shutoff member, disposed on a side of the storage member close to the first filter portion, the shutoff member being used to prevent the second accommodating space from being connected to the first accommodating space; and A pressurizing element, disposed on the storage element, and used to increase the pressure in the second accommodation space; The connecting pipe is connected to the other end of the storage element, and the height of the output end of the connecting pipe away from the ground is equal to or higher than the height of the intercepting element close to the ground.

5. The cell filtration method according to claim 3, characterized in that The filtering mechanism also includes: a filter tube, located in the third accommodating space and detachably disposed on the other end of the connecting tube, the filter tube having the filter channel, and a guide hole connected to the filter channel is opened on the side wall of the filter tube; and a flow guide, partly located in the third accommodation space and between the flow guide hole and the input end of the filter tube, and arranged on the filter tube around the side wall of the filter tube, the flow guide being used to receive the cell suspension output from the flow guide hole and output the cell suspension; Wherein, the filter element is located in the filter channel and below the guide hole, and is arranged on the side of the filter tube close to the connecting tube, and the filter element is used to filter the cell suspension transported from the output end of the connecting tube; the detection element is located in the filter channel and connected to the filter element, and is arranged on the filter tube, the detection element is evenly distributed along the circumference of the filter element, and at least two of the filter elements correspond to each other along the diameter direction of the filter element.

6. The cell filtration method according to claim 5, characterized in that The blockage clearing unit comprises: A fixing member, arranged on one end of the connecting pipe close to the filtering mechanism; a third accommodating member, detachably disposed on the fixing member, the third accommodating member having the third accommodating space, an output hole communicating with the third accommodating space being formed on a side of the third accommodating member close to the fixing member, and the flow guide member being partially located outside the third accommodating space through the output hole; a flushing device, located in the third accommodating space and rotatably disposed on a side of the third accommodating member away from the fixing member, the flushing device being used to flush a clogged portion when the filter element is clogged; and The image acquisition device is located in the third accommodating space and is arranged on a side of the third accommodating member away from the fixing member. The image acquisition device is used to acquire an image of the filter member.

7. The cell filtration method according to claim 1, characterized in that The filtering information includes a plurality of groups of filtering data corresponding to filtering time, each group of filtering data includes two corresponding filtering resistance information transmitted by the detection elements, and the filtering resistance information reflects the pressure on the filtering element.

8. The cell filtration method according to claim 7, characterized in that The determining the clogging position of the filter element based on the filtering information includes: The filtering resistance information is monitored in real time. When any filtering resistance information reflects a filtering resistance greater than a preset resistance, a time node corresponding to the filtering resistance information having a filtering resistance greater than the preset resistance is confirmed as a warning node, and a time period after a preset warning time with the warning node as a starting point is confirmed as a warning time period; confirming the warning time period and the filtering resistance information included in each group of filtering data within the warning time period as analysis information; Calculating respectively the average values ​​of the filtration resistances reflected by the respective filtration resistance information within the warning time period, and obtaining a plurality of warning average values ​​corresponding to the respective filtration resistance information within the warning time period; Determine whether the filtration resistance reflected by each of the warning means is within a preset resistance range; if the filtration resistance reflected by the warning means is not within the preset resistance range, obtain the blockage position based on the analysis information; if the filtration resistance reflected by each of the warning means is within the preset resistance range, obtain an operation instruction instructing the cell filtration device to continue operating; wherein the preset resistance range is a numerical range with a fluctuation of 5% above and below the preset resistance.

9. The cell filtration method according to claim 8, characterized in that The obtaining of the blockage location based on the analysis information comprises: Acquire a filter surface image; wherein the filter surface image includes a cross-sectional image and a plurality of detection points annotated on the cross-sectional image, the cross-sectional image is a filter surface image of the filter element, the positions of the detection points on the cross-sectional image reflect the relative positions of each detection element and the filter element, and each detection point corresponds to one piece of filtering resistance information; Respectively confirming each group of the filtered data corresponding to each time node in the warning time period as single-layer information; The blockage position is obtained based on all the single-layer information and the filter surface image.

10. The cell filtration method according to claim 9, characterized in that The obtaining the blockage position based on all the single-layer information and the filter surface image comprises: Based on each of the single-layer information, a plurality of single-layer analysis information are obtained respectively; wherein the single-layer analysis information includes a plurality of blockage point information, and each of the blockage point information corresponds to a set of the filtering data respectively; The blockage position is obtained based on all the single-layer analysis information and the filter surface image.

11. The cell filtration method according to claim 10, characterized in that The obtaining the blockage position based on all the single-layer analysis information and the filter surface image comprises: Obtaining the blocking range information based on all the single-layer analysis information and the filter surface image; wherein the blocking range information includes a plurality of blocking surfaces, each of the blocking surfaces corresponds to one of the single-layer analysis information, and the blocking surfaces include the filter surface image and the blocking area marked on the filter surface image; After stacking the filter surface images in the blockage range information along the same direction, the overlapping portion of the blockage areas in the filter surface images is identified as the blockage position.

12. The cell filtration method according to claim 11, characterized in that The obtaining of the blockage range information based on all the single-layer analysis information and the filter surface image includes: Analyze each of the single-layer analysis information respectively, take the center of the filter surface image as the starting point, extend the extension distance along the extension direction of each of the blocking point information in the single-layer analysis information respectively, and confirm the endpoint of each extension line as the blocking point; After connecting each adjacent blocking point along a straight line, the formed closed figure is confirmed as the blocking area, and the blocking area and the filter surface image are confirmed as the blocking surface; After all the single-layer analysis information is analyzed, all the blocked surfaces are confirmed as the blocked range information.

Images