A composition, application and cosmetic product resistant to heat stimulation and UV stimulation

Abstract

The present invention belongs to the field of cosmetics and discloses a composition, which includes prickly ash fruit extract, epigallocatechin gallate, diacetylboldine, sanguisorba root extract, and niacinamide. The composition has excellent antioxidant efficacy as detected by the DPPH method. At the same time, the present invention also discloses a composition for resisting heat stimulation and UV stimulation. By using a nematode model for detection, it can significantly inhibit the level of lipofuscin generation in nematodes after heat stimulation and ultraviolet light stimulation, and there is a significant synergistic effect on the inhibition of lipofuscin generation in nematodes after heat stimulation and ultraviolet light stimulation when the composition is used in combination with withania somnifera root extract. It shows that the composition of the present invention has very good anti-wrinkle effects of anti-photoaging and anti-thermal aging, and can effectively prevent the formation of skin spots.

Description

Technical Field

[0001] The present invention relates to the daily chemical field, and specifically to a composition, an application, and a cosmetic for resisting heat stimulation and UV stimulation. Background Art

[0002] The deposition of skin pigmentation is closely related to heat stimulation and light stimulation. Improving the cell's ability to respond to heat stimulation and light stimulation is an effective method to improve pigmentation;

[0003] However, we believe that the root cause of improving skin pigmentation is not only determined by the cell's ability to respond to heat stimulation and light stimulation, but also closely related to other factors, such as the ability to deposit and remove melanin and the cell's antioxidant ability. Among them, we believe that the cell's antioxidant ability is the basis for realizing the above-mentioned cell's ability to respond to heat stimulation and light stimulation, and the ability to deposit and remove melanin.

[0004] The technical problems to be solved in this case are: how to screen out a combination with better antioxidant ability, and how to develop an anti-wrinkle cosmetic raw material that can improve the cell's ability to respond to heat stimulation and light stimulation. Summary of the Invention

[0005] The purpose of the present invention is to provide a composition. The composition uses niacinamide with excellent melanin inhibition ability as the basic raw material, and is combined with the skin protectant component diacetylboldine. Using Zanthoxylum bungeanum fruit extract, epigallocatechin gallate, and Sanguisorba officinalis root extract as antioxidant synergistic components, the purpose of inhibiting melanin production and enhancing antioxidant ability can be achieved. Finally, the effects of removing spots and whitening, and anti-wrinkle are achieved.

[0006] At the same time, the present invention also discloses a composition for resisting heat stimulation and UV stimulation. On the basis of the above composition, combined with Withania somnifera root extract, it can effectively improve the cell's ability to respond to heat stimulation and light stimulation, and achieve the anti-wrinkle effect. In addition, the present invention also discloses the related applications of the above composition and the composition containing Withania somnifera root extract.

[0007] To achieve the above purpose, the present invention provides the following technical solutions:

[0008] A composition, comprising the following components in weight percentage:

[0009] Zanthoxylum bungeanum fruit extract 0.0001~0.05wt%;

[0010] Epigallocatechin gallate 0.0001~0.3wt%;

[0011] Diacetylboldine 0.0001~0.5wt%;

[0012] Sanguisorba officinalis root extract 0.0005 - 0.5 wt%;

[0013] Nicotinamide 1 - 5 wt%;

[0014] The balance of solvent.

[0015] The functions of the raw materials of the present invention are as follows:

[0016] Skin protectant ingredient: diacetylboldine; Melanin production - inhibiting ingredient: nicotinamide; Antioxidant - active ingredients: nicotinamide, Zanthoxylum bungeanum fruit extract, epigallocatechin gallate, Sanguisorba officinalis root extract; among them, Zanthoxylum bungeanum fruit extract, epigallocatechin gallate, and Sanguisorba officinalis root extract can significantly improve the antioxidant ability of nicotinamide.

[0017] The mechanism of the synergy of the antioxidant abilities of nicotinamide, Zanthoxylum bungeanum fruit extract, epigallocatechin gallate, and Sanguisorba officinalis root extract lies in:

[0018] Nicotinamide itself can neutralize the unpaired electrons of free radicals by providing hydrogen atoms, thereby reducing the damage of free radicals to cells; in addition, nicotinamide is a component of coenzyme nicotinamide adenine dinucleotide phosphate (Nicotinamide adeninedinucleotide phosphate, NADP), and nicotinamide adenine dinucleotide phosphate (NADPH) formed by a series of enzymatic reactions of NADP in cells can provide electrons for antioxidant enzymes to make them in a reduced state, so as to effectively scavenge free radicals.

[0019] The main active ingredient sanshool in Zanthoxylum bungeanum fruit extract can reduce the content of intracellular reactive oxygen species (ROS) and malondialdehyde by inhibiting the activity of oxidative stress enzymes.

[0020] Epigallocatechin gallate contains multiple phenolic hydroxyl groups in its structure, which can provide hydrogen atoms to react with free radicals to achieve direct antioxidant effects; epigallocatechin gallate can chelate metal ions such as iron ions (Fe 3+ ), and copper ions (Cu 2+ ), etc., reducing their activity in participating in free - radical generation reactions; epigallocatechin gallate can also up - regulate the activity of intracellular antioxidant enzymes and regulate the intracellular redox signaling pathway, thereby exerting antioxidant effects.

[0021] Sanguisorba officinalis root extract mainly contains various components with antioxidant activity, such as tannins, flavonoids, triterpenoids, etc. It also has multiple phenolic hydroxyl groups in its structure and has an antioxidant mechanism similar to that of epigallocatechin gallate.

[0022] By combining the above-mentioned components with antioxidant effects in different dimensions and verifying through the DPPH method, it is found that the present invention has a good antioxidant synergistic effect.

[0023] Meanwhile, the present invention also discloses a composition with anti-thermal stimulation and anti-UV stimulation effects, which includes the above-mentioned composition and Withania somnifera root extract.

[0024] The composition and Withania somnifera root extract cooperate synergistically to improve the cell's ability to respond to thermal stimulation and light stimulation. The mechanism is as follows:

[0025] Both thermal stimulation and light stimulation can lead to stress responses in cells. When cells are subjected to thermal stimulation or light stimulation, the content of ROS in cells usually increases, causing oxidative damage. The above-mentioned composition has excellent antioxidant effects. It is analyzed that it can improve the cell's ability to respond to thermal stimulation and light stimulation by reducing the production of ROS in cells. In addition, Withania somnifera root extract mainly contains withanolide components. Research shows that it can improve the adaptability and resilience of cells under heat stress, inhibit cortisol or up-regulate the content of happy factors such as dopamine, β-endorphin, serotonin, and oxytocin, and has a good anti-heat stress effect.

[0026] The present invention verifies through a nematode model test that the above-mentioned composition can reduce the level of lipofuscin under thermal stimulation and ultraviolet stimulation, and after being compounded with Withania somnifera root extract, it can further reduce the level of lipofuscin under thermal stimulation and ultraviolet stimulation, and can synergistically improve the cell's ability to respond to thermal stimulation and light stimulation.

[0027] In the above composition containing Withania somnifera root extract, the weight ratio of the composition to Withania somnifera extract is 1.0008 - 6.35:0.005 - 0.5.

[0028] In addition, the present invention also discloses the use of the above-mentioned composition in preparing cosmetics.

[0029] And, the use of the above-mentioned composition containing Withania somnifera root extract in preparing cosmetics is disclosed.

[0030] In the above use, the cosmetics are cosmetics with anti-photoaging, anti-thermal aging, and prevention of skin pigmentation effects.

[0031] Finally, the present invention also discloses a cosmetic, which contains the above-mentioned composition, or, contains the above-mentioned composition containing Withania somnifera root extract.

[0032] In the above cosmetic, the cosmetic contains 0.1 - 30 wt% of the composition, or, contains 0.1 - 30 wt% of the composition with anti-thermal stimulation and anti-UV stimulation effects.

[0033] Compared with the prior art, the beneficial effects of the present invention are:

[0034] The present invention uses nicotinamide, which has excellent melanin inhibitory ability, as the basic raw material, combines it with the skin protectant component diacetylboldine, and uses the prickly ash fruit extract, epigallocatechin gallate, and sanguisorba root extract as antioxidant synergistic components, so as to achieve the purpose of inhibiting melanin production and enhancing antioxidant ability.

[0035] On the basis of the above composition, the present invention combines with the withania somnifera root extract, which can effectively improve the ability of cells to respond to heat stimulation and light stimulation. Brief Description of the Drawings

[0036] Figure 1 It is the fluorescence photograph of the nematodes in the blank group in the nematode heat stimulation experiment;

[0037] Figure 2 It is the fluorescence photograph of the nematodes in the model group in the nematode heat stimulation experiment;

[0038] Figure 3 It is the fluorescence photograph of the nematodes in Example 7 in the nematode heat stimulation experiment;

[0039] Figure 4 It is the fluorescence photograph of the nematodes in Example 8 in the nematode heat stimulation experiment;

[0040] Figure 5 It is the fluorescence photograph of the nematodes in Example 9 in the nematode heat stimulation experiment;

[0041] Figure 6 It is the fluorescence photograph of the nematodes in Example 10 in the nematode heat stimulation experiment;

[0042] Figure 7 It is the fluorescence photograph of the nematodes in Example 11 in the nematode heat stimulation experiment;

[0043] Figure 8 It is the fluorescence photograph of the nematodes in Example 12 in the nematode heat stimulation experiment;

[0044] Figure 9 It is the fluorescence photograph of the nematodes in Comparative Example 8 in the nematode heat stimulation experiment;

[0045] Figure 10 It is the fluorescence photograph of the nematodes in Comparative Example 9 in the nematode heat stimulation experiment;

[0046] Figure 11 It is the fluorescence photograph of the nematodes in Comparative Example 10 in the nematode heat stimulation experiment;

[0047] Figure 12 It is the fluorescence photograph of the nematodes in the positive group in the nematode heat stimulation experiment;

[0048] Figure 13It is the fluorescence photo of nematodes in the blank group during the UV stimulation experiment of nematodes;

[0049] Figure 14 It is the fluorescence photo of nematodes in the model group during the UV stimulation experiment of nematodes;

[0050] Figure 15 It is the fluorescence photo of nematodes in Example 7 during the UV stimulation experiment of nematodes;

[0051] Figure 16 It is the fluorescence photo of nematodes in Example 8 during the UV stimulation experiment of nematodes;

[0052] Figure 17 It is the fluorescence photo of nematodes in Example 9 during the UV stimulation experiment of nematodes;

[0053] Figure 18 It is the fluorescence photo of nematodes in Example 10 during the UV stimulation experiment of nematodes;

[0054] Figure 19 It is the fluorescence photo of nematodes in Example 11 during the UV stimulation experiment of nematodes;

[0055] Figure 20 It is the fluorescence photo of nematodes in Example 12 during the UV stimulation experiment of nematodes;

[0056] Figure 21 It is the fluorescence photo of nematodes in Comparative Example 8 during the UV stimulation experiment of nematodes;

[0057] Figure 22 It is the fluorescence photo of nematodes in Comparative Example 9 during the UV stimulation experiment of nematodes;

[0058] Figure 23 It is the fluorescence photo of nematodes in Comparative Example 10 during the UV stimulation experiment of nematodes;

[0059] Figure 24 It is the fluorescence photo of nematodes in the positive group during the UV stimulation experiment of nematodes. Detailed implementation manners

[0060] Next, in combination with the embodiments of the present invention, the technical solutions of the present invention will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0061] Before elaborating on the method of the present invention, the sources of the reagents used in the present invention will be described first;

[0062] Prickly ash fruit extract solution: The content of sanshool is ≥15%; In this example, the prickly ash fruit extract solution is composed of 82.5 wt% of oleyl alcohol and 17.5 wt% of prickly ash fruit extract;

[0063] Epigallocatechin gallate glucoside solution: Selected from the product named Inoveol EGCG by Givaudan; In this example and the comparative example, the epigallocatechin gallate glucoside solution is composed of 0.75 wt% of epigallocatechin gallate glucoside and the balance of water;

[0064] Diacetylboldine solution: Selected from the product named LUMISKIN MBAL by Sederma; In this example and the comparative example, the diacetylboldine solution is composed of 0.1 wt% of diacetylboldine and the balance of caprylic / capric triglyceride;

[0065] Sanguisorba officinalis root extract solution: Selected from the product named Rootness Mood+ by Clariant Chemicals Co., Ltd.; In this example and the comparative example, the sanguisorba officinalis root extract solution is composed of 0.65 wt% of sanguisorba officinalis root extract and the balance of 1,3-propanediol;

[0066] The active ingredient of niacinamide is 100%;

[0067] Withania somnifera root extract solution: Selected from the product named Withania somnifera energy essence by Guangdong Heji Biotechnology Co., Ltd.; In this example and the comparative example, the Withania somnifera root extract solution is composed of 5.65 wt% of Withania somnifera root extract, 93.35 wt% of water and the balance of polyol as solubilizer;

[0068] The acryloyldimethyltaurate / VP copolymer is the product named ARISTOFLEX AVC by Clariant Chemicals Co., Ltd., which is used to assist in forming a relatively uniform mixture after compounding the composition.

[0069] Part 1 Preparation and Function Verification of the Composition

[0070] 1.1 Preparation of the Composition

[0071] The formula of the composition is as shown in Table 1 below;

[0072] The preparation method is as follows: First, weigh the acryloyldimethyltaurate / VP copolymer and an appropriate amount of water, mix and heat to 70 - 75 °C, cool to room temperature after mixing evenly, then add each component of the composition in the example and the comparative example in turn, and add water to 100 weight %, and homogenize and mix evenly with a homogenizing device to obtain the sample of the composition to be tested.

[0073] Table 1 Formula Table of the Composition Unit: g

[0074] Test group Prickly ash fruit extract solution Epigallocatechin gallate glucoside solution Diacetylboldine solution Sanguisorba officinalis root extract solution Nicotinamide Ammonium acryloyldimethyltaurate / VP copolymer Water Example 1 0.0001 0.0001 0.0001 0.0005 1 0.1 Add up to 100 Example 2 0.0001 0.0001 0.0001 0.0005 3 0.1 Add up to 100 Example 3 0.0001 0.0001 0.0001 0.0005 5 0.1 Add up to 100 Example 4 0.001 0.001 0.001 0.001 1 0.1 Add up to 100 Example 5 0.01 0.1 0.1 0.1 3 0.1 Add up to 100 Example 6 0.05 0.5 0.5 0.5 5 0.1 Add up to 100 Comparative example 1 0.0007 0 0.0001 0 1 0.1 Add up to 100 Comparative example 2 0 0.0007 0.0001 0 1 0.1 Add up to 100 Comparative example 3 0 0 0.0001 0.0007 1 0.1 Add up to 100 Comparative example 4 0.0002 0 0.0001 0.0005 1 0.1 Add up to 100 Comparative example 5 0 0.0002 0.0001 0.0005 1 0.1 Add up to 100 Comparative example 6 0.0003 0.0004 0.0001 0 1 0.1 Add up to 100 Comparative example 7 0 0 0.0001 0 1 0.1 Add up to 100

[0075] 1.2 Functional verification of the composition

[0076] 1.2.1 Safety test

[0077] Patch test method: Select a suitable patch tester. Using the closed patch test method, apply approximately 0.03 ml of the test substance in the patch tester, and externally apply a special tape to the back of the subject. After 24 hours, remove the test substance, and observe the skin reaction at 0.5, 24, and 48 hours after the patch test respectively. Record the results according to the skin reaction grading standard in the "Technical Specifications for Cosmetics Safety" (2015 Edition).

[0078] The test samples for this test are the samples of Examples 1-6 of the above composition.

[0079] Test results: There were no adverse reactions.

[0080] 1.2.2 DPPH free radical scavenging experiment and results

[0081] Take 2 mL of the above solution samples respectively and mix them thoroughly with 2 mL of 2×10 -4 mol / L DPPH solution prepared with absolute ethanol. After reacting in the dark at room temperature for 30 min, measure the absorbance at 517 nm to obtain A1; and measure the absorbance A2 of the mixture of 2 mL of the above solution sample and 2 mL of absolute ethanol and the absorbance A0 of the mixture of 2 mL of absolute ethanol according to the same operation. Calculate the DPPH free radical scavenging rate according to the following formula: DPPH free radical scavenging rate = [1 - (A1 - A2) / A0] × 100%.

[0082] The test samples for this test are the compositions in Examples 1-6 and Comparative Examples 1-7 above.

[0083] The test results are shown in Table 2 below;

[0084] Table 2 DPPH free radical scavenging experiment results of the composition

[0085] Test group DPPH free radical scavenging rate % Example 1 80.70 Example 2 81.13 Example 3 83.27 Example 4 90.73 Example 5 96.94 Example 6 98.41 Comparative example 1 70.07 Comparative example 2 72.35 Comparative example 3 69.85 Comparative example 4 75.95 Comparative example 5 76.08 Comparative example 6 78.42 Comparative example 7 57.06

[0086] Result analysis:

[0087] 1. It can be seen from Examples 1 to 3 in Table 2 that in the composition of the present invention, as the dosage of niacinamide increases, its DPPH scavenging rate increases. The reason for the limited increase is that when the dosage of niacinamide is 1%, its free radical scavenging ability is already relatively good. When the dosage is additionally increased, the free radical scavenging ability will not increase significantly.

[0088] 2. As can be seen from Examples 4 to 6 in Table 2, with the increase in the dosage of each component, the free radical scavenging ability increases significantly. As can be seen from Examples 1 to 6, within the optional range of the formulation dosage of the present invention, relatively excellent free radical scavenging effects can be achieved.

[0089] 3. As can be seen from Example 1, Comparative Example 1, Comparative Example 2, and Comparative Example 3 in Table 2, when any one of the prickly ash fruit extract solution, epigallocatechin gallate glucoside solution, and sanguisorba root extract solution appears, its free radical scavenging ability will decrease. Similarly, referring to Comparative Example 4, Comparative Example 5, and Comparative Example 6, a similar situation will occur, but the decrease will be slightly improved.

[0090] In Comparative Example 7, when the prickly ash fruit extract solution, epigallocatechin gallate glucoside solution, and sanguisorba root extract solution are not used, the free radical scavenging ability decreases significantly. Since the diacetylboldine solution does not have obvious free radical scavenging ability, it can also be learned from Comparative Example 7 that nicotinamide has good free radical scavenging ability when used alone.

[0091] We believe that good free radical scavenging ability is an important basis for inhibiting melanin production and improving the cell heat and radiation resistance of products. Based on the above research, the subsequent development and application of the composition are carried out.

[0092] 1.2.3 Melanin production experiment and results

[0093] Preparation of test samples: The solution sample of 0.2% by weight was prepared by diluting the composition of Example 1 with water for the subsequent melanin inhibition experiment (prepared and used immediately).

[0094] Melanin production test: After culturing B16-F10 cells in a plate for 16 h, the sample solvent was added to the blank control group, melanocyte stimulating hormone (MSH) was added to the model group, MSH and kojic acid were added to the positive group, and MSH and each concentration of the test sample were added to the experimental group, and the cells were cultured for another 65 h. The B16-F10 cells in different groups incubated with the sample for 65 h were digested with trypsin to obtain cell suspensions. The cell suspensions were taken for protein quantification. According to the protein quantification results, a certain amount of 1 M NaOH was added to each sample tube so that the total protein concentration in each tube was the same, and the absorbance was measured at 405 nm.

[0095] The melanin production rate and melanin production inhibition rate were calculated according to the following formulas:

[0096] Melanin production rate of the model group / sample group (%) = melanin amount of the model group / sample group / melanin amount of the blank group × 100%;

[0097] Melanin production inhibition rate (%) = (melanin production rate of the model group - melanin production rate of the sample group) / melanin production rate of the model group × 100%.

[0098] The test results are as follows:

[0099] The melanin production inhibition rate of the positive group (kojic acid) was 38.57%, and the melanin production inhibition rate of the sample group (Example 1) was 60.66%.

[0100] The concentration of Example 1 was the lowest, and its inhibitory effect on melanin production was significantly better than that of the positive group (kojic acid). Thus, it can be seen that the composition of the present invention has excellent melanin production inhibitory ability.

[0101] Part Two Preparation and Functional Verification of the Composition Against Heat Stimulation and UV Stimulation

[0102] 2.1 Preparation of the Composition Against Heat Stimulation and UV Stimulation

[0103] Prepare the combination in this part with reference to the preparation method in the first part;

[0104] Its formula refers to Table 3;

[0105] Table 3 Formula Table of the Composition Against Heat Stimulation and UV Stimulation Unit: g

[0106] Test group Prickly ash fruit extract solution Epigallocatechin gallate glucoside solution Diacetylboldine solution Sanguisorba officinalis root extract solution Nicotinamide Withania somnifera root extract solution Ammonium acryloyldimethyltaurate / VP copolymer Water Example 7 0.0001 0.0001 0.0001 0.0005 1 0.005 0.1 Add up to 100 Example 8 0.0001 0.0001 0.0001 0.0005 1 0.01 0.1 Add up to 100 Example 9 0.0001 0.0001 0.0001 0.0005 1 0.1 0.1 Add up to 100 Example 10 0.0001 0.0001 0.0001 0.0005 1 0.5 0.1 Add up to 100 Example 11 0.01 0.1 0.1 0.1 3 0.1 0.1 Add up to 100 Example 12 0.05 0.5 0.5 0.5 5 0.5 0.1 Add up to 100 Comparative example 8 0 0 0 0 0 0.005 0.1 Add up to 100 Comparative example 9 0.0001 0.0001 0.0001 0.0005 1 0 0.1 Add up to 100 Comparative example 10 0.0011 0.0011 0.0011 0.0025 1 0 0.1 Add up to 100

[0107] 2.2 Functional Verification of the Composition Against Heat Stimulation and UV Stimulation

[0108] Preparation of test samples: Prepare the samples of Examples 7 - 12 and Comparative Examples 8 - 10 above into 10% by weight solutions with water respectively to obtain each test sample group for the subsequent nematode experiments (prepared and used immediately).

[0109] 2.2.1 Nematode Heat Stimulation Experiment

[0110] Transfer the synchronized Caenorhabditis elegans at the L4 stage to a culture plate containing the sample and 5 - fluorouracil, and culture at 20°C for 4 days. After 4 days, place the Caenorhabditis elegans in the sample group, the positive group (curcumin), and the model group under heat treatment at 36°C for 3 h. The blank group is not subjected to heat treatment. After the heat stimulation treatment, collect and transfer the nematodes in each group to an agarose plate and anesthetize the nematodes. After the nematodes are anesthetized, observe the fluorescence intensity and take pictures under a fluorescence microscope (Nikon Ti) with an excitation wavelength of 340 - 380 nm and an emission wavelength of 430 nm, and use Image J image processing software to analyze and measure the autofluorescence intensity of lipofuscin in Caenorhabditis elegans.

[0111] 2.2.2 Nematode UV Stimulation Experiment

[0112] Transfer the synchronized Caenorhabditis elegans at the L4 stage to a culture plate containing the sample and 5-fluorouracil, and culture at 20 °C for 4 days. After 4 days, place the Caenorhabditis elegans in the sample group, positive group (curcumin), and model group under UVA with an irradiation dose of 10 J / cm² for 5 h, and the blank group is not treated with UVA irradiation. After the UVA irradiation treatment, collect the nematodes in each group and transfer them to an agarose plate to anesthetize the nematodes. After the nematodes are anesthetized, observe the fluorescence intensity and take pictures under a fluorescence microscope (Nikon Ti) with an excitation wavelength of 340 - 380 nm and an emission wavelength of 430 nm, and use Image J image processing software to analyze and measure the autofluorescence intensity of lipofuscin in Caenorhabditis elegans.

[0113] The inhibition rate of nematode lipofuscin production is calculated according to the following formula:

[0114] Inhibition rate of lipofuscin production (%) = (1 - T / C) × 100%;

[0115] T: Average fluorescence intensity value of the sample group;

[0116] C: Average fluorescence intensity value of the model group.

[0117] The test results of the composition against heat stimulation and UV stimulation are shown in Table 4 below;

[0118] Table 4 Test results of the composition against heat stimulation and UV stimulation

[0119]

[0120] In the accompanying drawings, Figure 1 is the fluorescence photograph of the nematodes in the blank group in the nematode heat stimulation experiment; Figure 2 is the fluorescence photograph of the nematodes in the model group in the nematode heat stimulation experiment; Figure 3 is the fluorescence photograph of the nematodes in Example 7 in the nematode heat stimulation experiment; Figure 4 is the fluorescence photograph of the nematodes in Example 8 in the nematode heat stimulation experiment; Figure 5 is the fluorescence photograph of the nematodes in Example 9 in the nematode heat stimulation experiment; Figure 6 is the fluorescence photograph of the nematodes in Example 10 in the nematode heat stimulation experiment; Figure 7 is the fluorescence photograph of the nematodes in Example 11 in the nematode heat stimulation experiment; Figure 8 is the fluorescence photograph of the nematodes in Example 12 in the nematode heat stimulation experiment; Figure 9 is the fluorescence photograph of the nematodes in Comparative Example 8 in the nematode heat stimulation experiment; Figure 10 is the fluorescence photograph of the nematodes in Comparative Example 9 in the nematode heat stimulation experiment; Figure 11 is the fluorescence photograph of the nematodes in Comparative Example 10 in the nematode heat stimulation experiment;Figure 12 It is the fluorescence photo of nematodes in the positive group during the heat stimulation experiment of nematodes;

[0121] Figure 13 It is the fluorescence photo of nematodes in the blank group during the UV stimulation experiment of nematodes; Figure 14 It is the fluorescence photo of nematodes in the model group during the UV stimulation experiment of nematodes; Figure 15 It is the fluorescence photo of nematodes in Example 7 during the UV stimulation experiment of nematodes; Figure 16 It is the fluorescence photo of nematodes in Example 8 during the UV stimulation experiment of nematodes; Figure 17 It is the fluorescence photo of nematodes in Example 9 during the UV stimulation experiment of nematodes; Figure 18 It is the fluorescence photo of nematodes in Example 10 during the UV stimulation experiment of nematodes; Figure 19 It is the fluorescence photo of nematodes in Example 11 during the UV stimulation experiment of nematodes; Figure 20 It is the fluorescence photo of nematodes in Example 12 during the UV stimulation experiment of nematodes; Figure 21 It is the fluorescence photo of nematodes in Comparative Example 8 during the UV stimulation experiment of nematodes; Figure 22 It is the fluorescence photo of nematodes in Comparative Example 9 during the UV stimulation experiment of nematodes; Figure 23 It is the fluorescence photo of nematodes in Comparative Example 10 during the UV stimulation experiment of nematodes; Figure 24 It is the fluorescence photo of nematodes in the positive group during the UV stimulation experiment of nematodes.

[0122] Result analysis:

[0123] 1. As can be seen from Comparative Example 8, the withania somnifera root extract solution itself has a certain ability to inhibit lipofuscin formation. As can be seen from Comparative Example 9 and Comparative Example 10, the inhibition ability of the composition is slightly lower than that of the withania somnifera extract; there is no significant difference between the two.

[0124] 2. As can be seen from Example 7, Example 8, Example 9, and Example 10, with the increase in the dosage of each substance, its inhibition ability is gradually improved;

[0125] 3. As can be seen from the comparison between Example 9 and Example 11 and between Example 10 and Example 12, the inhibition ability of Example 11 and Example 12 is significantly improved. From another aspect, it shows that when the dosage of the withania somnifera root extract solution reaches 0.5%, its performance has reached the limit. When the dosage of the composition is increased, it can improve the inhibition ability of the composition.

[0126] Summary:

[0127] 1. The composition of the present invention has excellent melanin inhibition ability, has a certain ability to resist cell heat stimulation and UV stimulation, and has relatively excellent synergy in DPPH scavenging ability, achieving the effects of removing freckles and whitening, and anti-wrinkle;

[0128] 2. On the basis of having the basic functions of the above composition, the composition of the present invention against heat stimulation and UV stimulation exhibits good abilities against cellular heat stimulation and UV stimulation. These two abilities mean that the composition of the present invention against heat stimulation and UV stimulation has a better ability to deal with skin aging problems such as age spots, achieving the effects of removing spots and whitening, and anti-wrinkle.

Claims

1. A composition, characterized in that The composition includes the following weight percentages: Zanthoxylum bungeanum fruit extract solution 0.0001~0.05wt%; Epigallocatechin galloside solution 0.0001~0.1wt%; Diacetyl boldine solution 0.0001~0.5wt%; Sanguisorba officinalis root extract solution 0.0005~0.5wt%; Nicotinamide 1~5wt%; The remaining amount of solvent; The Zanthoxylum bungeanum fruit extract solution contains 17.5 wt % of Zanthoxylum bungeanum fruit extract; The epigallocatechin galloside solution contains 0.75 wt % of epigallocatechin galloside; The diacetyl boldine solution contains 0.1 wt % of diacetyl boldine; The Sanguisorba officinalis root extract solution contains 0.65 wt % of Sanguisorba officinalis root extract.

2. A composition for resisting heat stimulation and UV stimulation, characterized in that: comprising the composition as claimed in claim 1 and a Withania somnifera root extract solution; The Withania somnifera root extract solution contains 5.65 wt % of Withania somnifera root extract; The weight ratio of the active ingredient in the composition to the Withania somnifera root extract solution is 1.0008-3.31:0.005-0.5; The effective ingredients in the composition are composed of Zanthoxylum bungeanum fruit extract solution, epigallocatechin galloside solution, diacetyl boldine solution, sanguisorba officinalis root extract solution and nicotinamide.

3. Use of the composition as claimed in claim 1 in preparing cosmetics.

4. Use of the composition as claimed in claim 2 in preparing cosmetics.

5. The use according to claim 4, characterized in that The cosmetics are cosmetics with the functions of resisting light aging, resisting heat aging and preventing the generation of pigmentation spots.

6. A cosmetic, characterized in that: Containing 0.1-30 wt% of the composition as claimed in claim 1, or, containing 0.1-30 wt% of the composition as claimed in claim 2.

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