Storage method and storage equipment for low-temperature cell storage

Abstract

The invention discloses a storage method and storage equipment for low-temperature cell storage, the storage equipment comprises a storage box, the right side of the storage box is fixedly connected with a refrigeration shell, and the interior of the refrigeration shell is fixedly connected with a refrigerator; a sealing cover is mounted at the upper end in the placing groove, and a through hole is formed in the lower end face of the placing groove; a cavity is formed in the lower end of the storage box, a power mechanism, a limiting mechanism and a blocking mechanism are arranged at the bottom end in the cavity, the blocking mechanism is matched with the through hole, and the power mechanism and the limiting mechanism are located at the lower ends of the containing grooves and correspond to the containing grooves one to one; a square hole is formed in the right end face of the storage box and communicates with the cavity. When needed cells are taken out, an overlarge opening does not need to be opened, so that leakage of cold air is effectively reduced, the through hole is blocked under the action of the blocking mechanism when the test tube is taken out, leakage of the cold air can be further reduced, and waste of resources is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of cell storage, and in particular to a storage method and storage equipment for low-temperature cell storage. Background Art

[0002] The main experimental objects of cell biology research are various primary cultured or subcultured cells or cell lines. In order to avoid contamination caused by long-term in vitro culture of cultured cells or gene mutation caused by multiple subcultures, the experimenters store the cultured cells at low temperatures during the vigorous growth period. In this low-temperature environment, the cells can be preserved for a long time.

[0003] Currently, when storing cells at low temperature, the test tubes containing cells are directly placed inside a storage box. Although this achieves the purpose of low-temperature storage, the large opening of the storage box is prone to loss of internal cold air during the retrieval process. At the same time, the temperature difference is likely to interfere with other cells inside, affecting the storage quality of the cells. Therefore, it is necessary to design a storage device for low-temperature cell storage. Summary of the invention

[0004] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a storage method and storage device for low-temperature cell storage.

[0005] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme: a storage device for storing low-temperature cells, including a storage box, a refrigeration shell is fixedly connected to the right side of the storage box, and a refrigerator is fixedly connected inside the refrigeration shell; a placement groove is opened on the upper end surface of the storage box, a sealing cover is installed at the upper end of the placement groove, and a through hole is opened on the lower end surface of the placement groove; a cavity is arranged inside the lower end of the storage box, and a power mechanism, a limiting mechanism and a blocking mechanism are arranged at the bottom end of the cavity, the blocking mechanism cooperates with the through hole, and the power mechanism and the limiting mechanism are both located at the lower end of the placement groove, and correspond one-to-one to the placement groove; a square hole is opened on the right end surface of the storage box, and the square hole is connected to the cavity.

[0006] As a further description of the above technical solution:

[0007] The blocking mechanism includes a fixed frame, a movable rod, a first spring, a connecting rod, a first movable plate, a sealing ring and a sealing ball. The fixed frame and the sealing ring are fixedly connected to the top of the cavity, the sealing ring corresponds to the through hole, the movable rod is slidably connected inside the fixed frame, the upper end of the movable rod is fixedly connected to the sealing ball, the sealing ball corresponds to the through hole, the upper end of the sealing ball is fixedly connected to the connecting rod, and the upper end of the connecting rod is fixedly connected to the first movable plate; a first spring is provided on the circumferential surface of the movable rod, the upper end of the first spring is fixedly connected to the sealing ball, and the lower end of the first spring is fixedly connected to the fixed frame.

[0008] As a further description of the above technical solution:

[0009] The diameter of the first movable plate is smaller than the diameter of the placement groove, and a circular hole is formed on the upper surface of the first movable plate.

[0010] As a further description of the above technical solution:

[0011] The power mechanism includes a square tube, a second movable plate, a transmission rod and an electromagnet. The square tube is fixedly connected to the bottom end of the cavity, the second movable plate is slidably connected to the inside of the square tube, and both sides of the second movable plate pass through the square tube. The square tube is fixedly connected to the inside of the square tube, and the transmission rods are rotatably connected to both sides of the upper end of the second movable plate.

[0012] As a further description of the above technical solution:

[0013] The limiting mechanism includes a square rod, a limiting hole, a movable frame, a guide rod, a second spring, a guide block and a limiting rod, the lower end of the movable rod is fixedly connected to the square rod, the side end surface of the square rod is provided with a limiting hole, the square rod corresponds to the square tube and is slidably connected to the square tube; the end of the transmission rod away from the second movable plate is rotatably connected to the movable frame, the inside of the movable frame is fixedly connected to the guide rod, and two guide rods are provided, the circumferential surfaces of the two guide rods are slidably connected to the guide blocks, and the limiting rod is fixedly connected between the two guide blocks, the limiting rod passes through the movable frame and the square tube, and is slidably connected to the movable frame and the square tube, and the limiting rod corresponds to the limiting hole; the circumferential surface of the guide rod is provided with a second spring, one end of the second spring is fixedly connected to the movable frame, and the other end of the second spring is fixedly connected to the guide block.

[0014] As a further description of the above technical solution:

[0015] Both sides of the square tube are fixedly connected to limiting grooves, the limiting grooves are slidably connected to limiting rods, the limiting rods are fixedly connected to the moving frame, the lower ends of the limiting grooves are fixedly connected to support rods, and the support rods are fixedly connected to the square tube.

[0016] As a further description of the above technical solution:

[0017] A third spring is fixedly connected between the second moving plate and the electromagnet, and the elastic coefficient of the third spring is greater than the elastic coefficient of the second spring.

[0018] As a further description of the above technical solution:

[0019] A card slot is provided on the inner wall of the placement slot, a square slot is provided on the upper end surface of the storage box, the square slot is communicated with the card slot, a card block is engaged inside the card slot, the card block is fixedly connected to the sealing cover, and a handle is fixedly connected to the upper end of the sealing cover.

[0020] As a further description of the above technical solution:

[0021] A sealing ring is sleeved on the circumferential surface of the sealing cover, and the sealing ring is located at the lower end of the sealing cover.

[0022] A storage method for cryogenic cell storage, using the storage device for cryogenic cell storage described above, comprises the following steps:

[0023] S1: Open the sealing cover and insert the test tube containing cells into the installation slot;

[0024] S2: The insertion of the test tube opens the blocking mechanism, and the cold air enters the placement slot along the through hole;

[0025] S3: The refrigerator is working to produce cold air, so that the test tubes containing cells are always kept at a low temperature.

[0026] The present invention has the following beneficial effects:

[0027] 1. Compared with the prior art, the present invention provides a placement groove, a sealing cover and a blocking mechanism. Since the placement groove and the sealing cover are separately provided and correspond one to one, when taking out the required cells, it is only necessary to open the corresponding sealing cover, which makes it convenient to take out the test tube containing the cells in the placement groove, so that when taking out the required cells, there is no need to open an opening that is too large, thereby effectively reducing the leakage of cold air. As the test tube is taken out, the through hole is also blocked under the action of the blocking mechanism, so that the leakage of cold air can be further reduced, thereby reducing the waste of resources.

[0028] 2. Compared with the prior art, the storage method and storage device for low-temperature cell storage use a square rod, a limiting hole, a movable frame, a guide rod, a second spring, a guide block and a limiting rod. When the test tube is inserted, when the test tube contacts the first movable plate, the first movable plate will be squeezed to move, thereby driving the square rod to move toward the inside of the square tube. When the square rod contacts the limiting rod, the limiting rod will be squeezed to move toward the outside of the square tube, thereby driving the guide block to slide on the guide rod, thereby compressing the second spring. When the limiting rod reaches the limiting hole position, the limiting rod can be reset under the action of the compressed second spring, so that the limiting rod is inserted into the limiting hole, thereby realizing the limitation of the square rod, so that the blocking ball is stably in a state away from the through hole, which is conducive to the stable delivery of cold air to the inside of the placement groove; at the same time, the limitation of the square rod also reduces the squeezing of the test tube by the first movable plate, which is conducive to reducing damage to the test tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0030] Figure 2 It is a schematic diagram of the internal structure of the present invention;

[0031] Figure 3 For the present invention Figure 2 Enlarged view of part A;

[0032] Figure 4 It is a structural schematic diagram of the storage box of the present invention;

[0033] Figure 5 For the present invention Figure 4 Enlarged view of part B;

[0034] Figure 6 It is a structural schematic diagram of the sealing cover of the present invention;

[0035] Figure 7 It is a schematic diagram of the structure of the power mechanism, the limiting mechanism and the blocking mechanism of the present invention cooperating with each other;

[0036] Figure 8 For the present invention Figure 7 Enlarged view of part C in the middle;

[0037] Fig. 9 It is a schematic diagram of the structure inside the power mechanism of the present invention;

[0038] Fig.10 For the present invention Fig. 9 Enlarged view of part D in the middle;

[0039] Fig.11 It is a schematic structural diagram of the square tube of the present invention.

[0040] Legend:

[0041] 1. Storage box; 2. Refrigeration shell; 3. Sealing cover; 4. Refrigeration machine; 5. Placement slot; 6. Cavity; 7. Blocking mechanism; 701. Fixing frame; 702. Moving rod; 703. First spring; 704. Connecting rod; 705. First moving plate; 706. Sealing ring; 707. Sealing ball; 8. Limiting mechanism; 801. Square rod; 802. Limiting hole; 803. Moving frame; 804. Guide rod; 805 , second spring; 806, guide block; 807, limiting rod; 9, power mechanism; 901, square tube; 902, second movable plate; 903, transmission rod; 904, electromagnet; 10, through hole; 11, square hole; 12, circular hole; 13, slot; 14, square slot; 15, handle; 16, block; 17, sealing ring; 18, limiting rod; 19, limiting slot; 20, support rod; 21, third spring. DETAILED DESCRIPTION

[0042] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0043] Reference Figure 1-11 The present invention provides a storage device for storing low-temperature cells, comprising a storage box 1, a refrigeration shell 2 is fixedly connected to the right side of the storage box 1, and a refrigerator 4 is fixedly connected inside the refrigeration shell 2; a placement groove 5 is opened on the upper end surface of the storage box 1, a sealing cover 3 is installed at the upper end of the placement groove 5, and a through hole 10 is opened on the lower end surface of the placement groove 5; a cavity 6 is arranged inside the lower end of the storage box 1, and a power mechanism 9, a limiting mechanism 8 and a blocking mechanism 7 are arranged at the bottom end of the cavity 6, and the blocking mechanism 7 cooperates with the through hole 10, and the power mechanism 9 and the limiting mechanism 8 are both located at the lower end of the placement groove 5, and correspond one to one with the placement groove 5; a square hole 11 is opened on the right end surface of the storage box 1, and the square hole 11 is connected to the cavity 6.

[0044] Specifically, when in use, the cold air generated by the refrigerator 4 enters the cavity 6 of the storage box 1 through the square hole 11, then the sealing cover 3 is opened, and then the test tube containing the cells is inserted into the placement slot 5. As the test tube is inserted, the blocking mechanism 7 is also opened, so that the cold air in the cavity 6 will enter the placement slot 5 along the through hole 10, so that the cells are stored in a low temperature state. At the same time, the opening of the blocking mechanism 7, with the cooperation of the limiting mechanism 8, can make the blocking mechanism 7 always stably in the open state; when it is necessary to take out the required cells, the sealing cover 3 is opened first, and then the power mechanism 9 works, so that the limiting mechanism 8 no longer limits the blocking mechanism 7, so that it is convenient for the blocking mechanism 7 to block the through hole 10, so that the leakage of cold air can be reduced, and because the blocking mechanism 7 corresponds to the placement slot 5 one by one, when the placement slot 5 does not place the test tube containing the cells, the blocking mechanism 7 is always in the closed device, so as to effectively prevent the cold air from entering the empty placement slot 5, thereby reducing the waste of cold air.

[0045] The blocking mechanism 7 includes a fixed frame 701, a moving rod 702, a first spring 703, a connecting rod 704, a first moving plate 705, a sealing ring 706 and a sealing ball 707. The top of the cavity 6 is fixedly connected with the fixed frame 701 and the sealing ring 706, and the sealing ring 706 corresponds to the through hole 10. The moving rod 702 is slidably connected inside the fixed frame 701, and the upper end of the moving rod 702 is fixedly connected with the sealing ball 707, and the sealing ball 707 corresponds to the through hole 10. The upper end of the sealing ball 707 is fixedly connected with the connecting rod 704, and the upper end of the connecting rod 704 is fixedly connected with the first moving plate 705; the circumferential surface of the moving rod 702 is provided with a first spring 703, the upper end of the first spring 703 is fixedly connected to the sealing ball 707, and the lower end of the first spring 703 is fixedly connected to the fixed frame 701 During operation, as the test tube is inserted, when the test tube contacts the first movable plate 705, the first movable plate 705 will be squeezed to move, and then with the cooperation of the connecting rod 704, the sealing ball 707 can be driven away from the sealing ring 706, so that the sealing ball 707 is no longer in a sealed state, so that the cold air can enter the placement groove 5 through the through hole 10, which is conducive to the test tube containing cells in the placement groove 5 being in a low temperature state. At the same time, the movement of the sealing ball 707 also drives the moving rod 702 to slide inside the fixed frame 701, thereby compressing the first spring 703, and the compressed first spring 703 is conducive to the reset of the first movable plate 705. With the reset of the first movable plate 705, it is convenient to drive the test tube out of the placement groove 5, which is conducive to taking out the test tube.

[0046] The diameter of the first movable plate 705 is smaller than the diameter of the placement groove 5 , and a circular hole 12 is provided on the upper end surface of the first movable plate 705 . When working, the circular hole 12 facilitates cold air to enter the placement groove 5 .

[0047] The power mechanism 9 includes a square cylinder 901, a second movable plate 902, a transmission rod 903 and an electromagnet 904. The square cylinder 901 is fixedly connected to the bottom end of the cavity 6, the second movable plate 902 is slidably connected to the inside of the square cylinder 901, and the second movable plate 902 passes through the square cylinder 901 on both sides. The electromagnet 904 is fixedly connected to the inside of the square cylinder 901, and the transmission rod 903 is rotatably connected to both sides of the upper end of the second movable plate 902. During operation, when it is necessary to take out the test tube with cells, the electromagnet 904 works, so that the second movable plate 902 approaches the electromagnet 904, thereby driving the transmission rod 903 to move.

[0048] The limiting mechanism 8 includes a square rod 801, a limiting hole 802, a moving frame 803, a guide rod 804, a second spring 805, a guide block 806 and a limiting rod 807. The lower end of the moving rod 702 is fixedly connected to the square rod 801, and the side end surface of the square rod 801 is provided with a limiting hole 802. The square rod 801 corresponds to the square tube 901 and is slidably connected to the square tube 901; the end of the transmission rod 903 away from the second moving plate 902 is rotatably connected to the moving frame 803, and the inside of the moving frame 803 is fixedly connected to the guide rod 804, and the guide rod 804 is provided with two. The circumferential surface of 804 is slidably connected with a guide block 806, and a limiting rod 807 is fixedly connected between the two guide blocks 806. The limiting rod 807 penetrates the movable frame 803 and the square tube 901, and is slidably connected between the movable frame 803 and the square tube 901. The limiting rod 807 corresponds to the limiting hole 802; the circumferential surface of the guide rod 804 is provided with a second spring 805, one end of the second spring 805 is fixedly connected to the movable frame 803, and the other end of the second spring 805 is fixedly connected to the guide block 806. When working, as the transmission rod 903 moves, the movable frame 803 can be driven to move , thereby driving the limiting rod 807 to move outward, so that the limiting rod 807 is separated from the limiting hole 802, thereby releasing the fixation of the square rod 801, thereby facilitating the cooperation between the sealing ball 707 and the sealing ring 706 to achieve a sealed state; when inserting the test tube, when the test tube contacts the first movable plate 705, the first movable plate 705 will be squeezed to move, thereby driving the square rod 801 to move inside the square tube 901, and when the square rod 801 contacts the limiting rod 807, the limiting rod 807 will be squeezed to move outside the square tube 901, thereby driving the guide block 806 in the guide The limiting rod 807 slides on the rod 804, thereby compressing the second spring 805. When the limiting rod 807 reaches the limiting hole 802, the limiting rod 807 can be reset under the action of the compressed second spring 805, so that the limiting rod 807 is inserted into the limiting hole 802, thereby realizing the limitation of the square rod 801, so that the blocking ball is stably in a state away from the through hole 10, which is beneficial to the stable transportation of cold air to the inside of the placement slot 5; at the same time, the limitation of the square rod 801 also reduces the squeezing of the test tube by the first movable plate 705, which is beneficial to reduce damage to the test tube.

[0049] The square tube 901 is fixedly connected to the limiting groove 19 on both sides, and the limiting rod 18 is slidably connected inside the limiting groove 19. The limiting rod 18 is fixedly connected to the movable frame 803, and the lower end of the limiting groove 19 is fixedly connected to the support rod 20, and the support rod 20 is fixedly connected to the square tube 901. When working, by setting the limiting groove 19 and the limiting rod 18, the movement of the movable frame 803 can be limited, so that the movable frame 803 can move smoothly.

[0050] A third spring 21 is fixedly connected between the second movable plate 902 and the electromagnet 904. The elastic coefficient of the third spring 21 is greater than the elastic coefficient of the second spring 805. During operation, by setting the third spring 21, as the second movable plate 902 moves upward, the third spring 21 will be compressed, and the compressed third spring 21 is conducive to resetting the second movable plate 902. At the same time, since the elastic coefficient of the third spring 21 is greater than the elastic coefficient of the second spring 805, this is also conducive to the cooperation between the limiting rod 807 and the limiting hole 802.

[0051] A card slot 13 is provided on the inner wall of the placement groove 5, and a square groove 14 is provided on the upper end surface of the storage box 1. The square groove 14 is connected to the card slot 13, and a card block 16 is engaged inside the card slot 13. The card block 16 is fixedly connected to the sealing cover 3, and a handle 15 is fixedly connected to the upper end of the sealing cover 3. When working, hold the handle 15 by hand, and then align the card block 16 on the sealing cover 3 with the square groove 14. At this time, the card block 16 can be inserted into the card slot 13 along the square groove 14. After the card block 16 reaches the inside of the card slot 13, the sealing cover 3 is rotated to make the card block 16 engage with the card slot 13, so that the sealing cover 3 is stably installed inside the placement groove 5.

[0052] A sealing ring 17 is sleeved on the circumferential surface of the sealing cover 3 , and the sealing ring 17 is located at the lower end of the sealing cover 3 . During operation, the sealing ring 17 can be provided to improve the sealing effect of the sealing cover 3 with the cooperation of the sealing ring 17 .

[0053] A storage method for cryogenic cell storage, using the storage device for cryogenic cell storage described above, comprises the following steps:

[0054] S1: Open the sealing cover 3 and insert the test tube containing cells into the installation groove;

[0055] S2: The insertion of the test tube opens the blocking mechanism 7, and the cold air enters the placement groove 5 along the through hole 10;

[0056] S3: Refrigerator 4 works to produce cold air all the time, so that the test tube containing the cells is always in a low temperature state.

[0057] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A storage device for cryogenic cell storage, comprising a storage box (1), characterized in that: The right side of the storage box (1) is fixedly connected to a refrigeration shell (2), and a refrigerator (4) is fixedly connected inside the refrigeration shell (2); a placement groove (5) is provided on the upper end surface of the storage box (1), a sealing cover (3) is installed at the upper end of the placement groove (5), and a through hole (10) is provided on the lower end surface of the placement groove (5); a cavity (6) is provided inside the lower end of the storage box (1), and a power mechanism (9), a limiting mechanism (8) and a blocking mechanism (7) are provided at the bottom end of the cavity (6), and the blocking mechanism (7) cooperates with the through hole (10), and the power mechanism (9) and the limiting mechanism (8) are both located at the lower end of the placement groove (5) and correspond one to one with the placement groove (5); a square hole (11) is provided on the right end surface of the storage box (1), and the square hole (11) is connected to the cavity (6).

2. A storage device for cryogenic cell storage according to claim 1, characterized in that: The blocking mechanism (7) comprises a fixed frame (701), a movable rod (702), a first spring (703), a connecting rod (704), a first movable plate (705), a sealing ring (706) and a sealing ball (707); the top of the cavity (6) is fixedly connected with the fixed frame (701) and the sealing ring (706); the sealing ring (706) corresponds to the through hole (10); the movable rod (702) is slidably connected to the inside of the fixed frame (701); the movable rod (702) is A sealing ball (707) is fixedly connected to the end, the sealing ball (707) corresponds to the through hole (10), the upper end of the sealing ball (707) is fixedly connected to a connecting rod (704), and the upper end of the connecting rod (704) is fixedly connected to a first movable plate (705); a first spring (703) is provided on the circumferential surface of the movable rod (702), the upper end of the first spring (703) is fixedly connected to the sealing ball (707), and the lower end of the first spring (703) is fixedly connected to the fixed frame (701).

3. A storage device for cryogenic cell storage according to claim 2, characterized in that: The diameter of the first movable plate (705) is smaller than the diameter of the placement groove (5), and a circular hole (12) is provided on the upper end surface of the first movable plate (705).

4. A storage device for cryogenic cell storage according to claim 2, characterized in that: The power mechanism (9) comprises a square tube (901), a second movable plate (902), a transmission rod (903) and an electromagnet (904); the square tube (901) is fixedly connected to the bottom end of the cavity (6); the second movable plate (902) is slidably connected to the inside of the square tube (901), and both sides of the second movable plate (902) pass through the square tube (901); the electromagnet (904) is fixedly connected to the inside of the square tube (901); and the transmission rod (903) is rotatably connected to both sides of the upper end of the second movable plate (902).

5. A storage device for cryogenic cell storage according to claim 4, characterized in that: The limiting mechanism (8) comprises a square rod (801), a limiting hole (802), a movable frame (803), a guide rod (804), a second spring (805), a guide block (806) and a limiting rod (807); the lower end of the movable rod (702) is fixedly connected to the square rod (801); the side end surface of the square rod (801) is provided with a limiting hole (802); the square rod (801) corresponds to the square tube (901) and is slidably connected to the square tube (901); the end of the transmission rod (903) away from the second movable plate (902) is rotatably connected to the movable frame (803); the movable frame (803) is fixedly connected to the guide rod (804) inside; Two guide rods (804) are provided, and the circumferential surfaces of the two guide rods (804) are slidably connected with guide blocks (806), and a limiting rod (807) is fixedly connected between the two guide blocks (806). The limiting rod (807) passes through the movable frame (803) and the square tube (901), and is slidably connected between the movable frame (803) and the square tube (901), and the limiting rod (807) corresponds to the limiting hole (802); a second spring (805) is provided on the circumferential surface of the guide rod (804), one end of the second spring (805) is fixedly connected to the movable frame (803), and the other end of the second spring (805) is fixedly connected to the guide block (806).

6. A storage device for cryogenic cell storage according to claim 5, characterized in that: Both sides of the square tube (901) are fixedly connected to limit grooves (19), the limit grooves (19) are slidably connected to limit rods (18), the limit rods (18) are fixedly connected to the movable frame (803), the lower ends of the limit grooves (19) are fixedly connected to support rods (20), and the support rods (20) are fixedly connected to the square tube (901).

7. A storage device for cryogenic cell storage according to claim 5, characterized in that: A third spring (21) is fixedly connected between the second movable plate (902) and the electromagnet (904), and the elastic coefficient of the third spring (21) is greater than the elastic coefficient of the second spring (805).

8. A storage device for cryogenic cell storage according to claim 1, characterized in that: The inner wall of the placement groove (5) is provided with a card slot (13), the upper end surface of the storage box (1) is provided with a square groove (14), the square groove (14) is communicated with the card slot (13), a card block (16) is engaged inside the card slot (13), the card block (16) is fixedly connected to the sealing cover (3), and a handle (15) is fixedly connected to the upper end of the sealing cover (3).

9. A storage device for cryogenic cell storage according to claim 1, characterized in that: A sealing ring (17) is sleeved on the circumferential surface of the sealing cover (3), and the sealing ring (17) is located at the lower end of the sealing cover (3).

10. A storage method for cryogenic cell storage, characterized in that: Using the storage device for cryogenic cell storage according to any one of claims 1 to 9 comprises the following steps: S1: Open the sealing cover (3) and insert the test tube containing cells into the installation groove; S2: The insertion of the test tube opens the blocking mechanism (7), and the cold air enters the placement groove (5) along the through hole (10); S3: The refrigerator (4) keeps producing cold air, so that the test tubes containing cells are kept at a low temperature.

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