A device for glucose clamp test based on a negative feedback model

Abstract

The present invention relates to the technical field of glucose clamp test, and in particular to a glucose clamp test device based on a negative feedback model, which includes a table body. At the front edge of the upper surface of the table body, a test box is fixedly installed. On both sides of the front end of the test box, a box door is respectively rotatably installed. Inside the test box, a fixed clamping plate is connected through an XY-axis moving mechanism. The upper and lower ends of the fixed clamping plate are respectively fixedly connected with an upper connecting plate and a lower connecting plate. One side of the fixed clamping plate is connected with a movable clamping plate through a plugging mechanism. A sliding opening is penetrated through the upper surface of the upper connecting plate. Inside the sliding opening, two sliders are respectively slidably inserted through an elastic meshing mechanism. The lower end of the slider is fixedly connected with a pressing plate. The present invention has the function of positioning white rats, and there is no need to anesthetize or tie up white rats during the test, which is convenient to operate; the inserted needle can be firmly positioned to prevent the problem of detachment and loosening; it can effectively prevent the problem that the hind legs of white rats touch the connecting tube, thereby blocking the test.

Description

Technical Field

[0001] The present invention relates to the technical field of glucose clamp test, and particularly to a glucose clamp test device based on a negative feedback model. Background Art

[0002] When developing insulin drugs, the glucose clamp test is an important method. According to different research purposes, the glucose clamp technique is divided into high glucose clamp, euglycemic clamp, and low glucose clamp. Among them, the glucose clamp test is currently recognized as the best method for evaluating the PK / PD characteristics of insulin and is widely used in the clinical research and development of insulin drugs.

[0003] In the euglycemic clamp test, the hypoglycemic effect caused by the increase in blood insulin concentration (by exogenous administration of the test or non-test insulin preparation) is antagonized by continuously adjusted intravenous glucose infusion, so that endogenous insulin secretion is effectively inhibited, and at the same time, the blood glucose level is "clamped" within a pre-determined target range. When the "clamp" test system is stable, after administering the test insulin drug, on the one hand, the PK characteristics can be described by detecting the change in the concentration of the test drug in plasma over time; on the other hand, the time-action curve of the test drug can be plotted by the change in the glucose infusion rate required to maintain the blood glucose concentration stable within the target range during the clamp test to describe its PD characteristics.

[0004] Traditional test operation management is complex and requires high skills of medical staff. Especially during the process of adjusting glucose injection, the current methods are all based on doctors' experience judgment to adjust the injection amount of the next glucose. How to accurately control the glucose injection rate to achieve the best measurement effect is a technical problem. Therefore, the method of adjusting the glucose injection amount in the glucose clamp test through a negative feedback model is relatively common. This method simplifies the test process, constructs a mathematical model to optimize the glucose injection amount to adjust the accuracy of the test, and can further optimize the model based on the measured data after the test to make the next test closer to the real drug effect data. However, in the white rat test, it is still necessary to intermittently perform needle insertion for injection and needle insertion for blood sampling and detection, which is quite inconvenient. At the same time, for the convenience of operation, it is necessary to paralyze or tie up the white rats before the experiment, which is also rather cumbersome and inconvenient. Summary of the Invention

[0005] The purpose of the present invention is to solve the deficiencies in the background art and propose a glucose clamp test device based on a negative feedback model.

[0006] To achieve the above object, the technical solution adopted by the present invention is as follows: A glucose clamp test device based on a negative feedback model, comprising a table body, a test box is fixedly installed at the front edge of the upper surface of the table body, box doors are respectively rotatably installed on both sides of the front end of the test box, a fixed clamp plate is connected to the inside of the test box through an XY-axis moving mechanism, upper and lower connecting plates are respectively fixedly connected to the upper and lower ends of the fixed clamp plate, a movable clamp plate is connected to one side of the fixed clamp plate through a plugging mechanism, a sliding opening is formed through the upper surface of the upper connecting plate, two sliders are slidably inserted into the inside of the sliding opening through an elastic meshing mechanism, a pressing plate is fixedly connected to the lower end of the slider, pressing feet are respectively fixedly connected to both sides of the lower surface of the pressing plate, needles are respectively arranged below every two pressing feet, and a needle handle is fixedly arranged on the outer surface of the needle.

[0007] Preferably, the XY-axis moving mechanism includes guide rails fixedly connected to the front and rear edges of the upper surface of the test box respectively, guide blocks are slidably clamped inside the guide rails, two guide rods are fixedly connected between the front and rear guide blocks, a connecting rod is fixedly connected to the outer surface of the fixed clamp plate, two sleeves are fixedly embedded at the upper end of the connecting rod, and the sleeves are slidably sleeved on the outer surface of the connecting rod.

[0008] Preferably, a connecting pipe is fixedly connected to the upper end of the needle, a wire-passing plate extending rearward is fixedly connected to the upper surface of the connecting rod, and the connecting pipe penetrates through the outer surface of the wire-passing plate.

[0009] Preferably, an injection mechanism is connected to the end of one of the connecting pipes, a detection mechanism is connected to the end of the other connecting pipe, the injection mechanism and the detection mechanism are respectively fixedly installed on the upper surface of the table body, and a main control module is fixedly installed on the upper surface of the table body.

[0010] Preferably, the elastic meshing mechanism includes lower guide plates fixedly connected to the front and rear surfaces of the slider respectively, the upper surface of the lower guide plate is slidably attached to the lower surface of the upper connecting plate, a lifting plate is slidably sleeved on the outer surface of the slider through an elastic mechanism, a plurality of limiting teeth are fixedly connected to the lower surface of the lifting plate, a limiting tooth groove is formed on the upper surface of the upper connecting plate, and the limiting teeth are engaged into the inside of the limiting tooth groove.

[0011] Preferably, the elastic mechanism includes a top connecting plate fixedly connected to the upper end of the slider, a reed is fixedly connected to the lower surface of the top connecting plate, the lower end of the reed is fixedly connected to the upper surface of the lifting plate, a plurality of lifting columns are fixedly connected to the upper surface of the lifting plate, the lifting columns slide through the lower surface of the top connecting plate, and a pulling plate is fixedly connected to the upper ends of the plurality of lifting columns together.

[0012] Preferably, a splicing plate is fixedly connected to the lower end of the movable clamping plate. A socket is penetrated and opened at the edge of the lower surface of the splicing plate. A threaded column is fixedly connected to the lower surface of the lower connecting plate. The threaded column slidably passes through the inside of the socket. A positioning sleeve is threadedly sleeved on the lower end of the threaded column. The upper surface of the positioning sleeve contacts the lower surface of the splicing plate.

[0013] Preferably, a first cushion pad is fixedly connected to the inner wall of the fixed clamping plate and the upper surface of the lower connecting plate together, and a second cushion pad is fixedly connected to the inner wall of the movable clamping plate.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] 1. The present invention has the function of positioning white rats, and there is no need to anesthetize or tie up white rats during the experiment, which is convenient to operate;

[0016] 2. The present invention can firmly position the inserted needle and prevent the problem of detachment and loosening;

[0017] 3. The present invention can effectively prevent the problem that the hind legs of white rats touch the connecting pipe, thereby blocking the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 is a schematic structural diagram of an apparatus for glucose clamp test based on a negative feedback model of the present invention;

[0019] Figure 2 is a schematic view of the test chamber of an apparatus for glucose clamp test based on a negative feedback model of the present invention;

[0020] Figure 3 is a schematic view of the movable clamping plate and the fixed clamping plate of an apparatus for glucose clamp test based on a negative feedback model of the present invention;

[0021] Figure 4 is an apparatus for glucose clamp test based on a negative feedback model of the present invention Figure 3 enlarged view at A;

[0022] Figure 5 is a schematic view of the slider of an apparatus for glucose clamp test based on a negative feedback model of the present invention;

[0023] Figure 6 is a schematic view when the positioning sleeve of an apparatus for glucose clamp test based on a negative feedback model of the present invention is unscrewed;

[0024] Figure 7 is a schematic view of the connecting pipe distribution of an apparatus for glucose clamp test based on a negative feedback model of the present invention.

[0025] In the drawings, the list of components represented by each reference numeral is as follows:

[0026] 1. Frustum; 2. Test chamber; 3. Chamber door; 4. Fixed clamping plate; 5. Movable clamping plate; 6. First cushion; 7. Second cushion; 8. Lower connecting plate; 9. Upper connecting plate; 10. Splicing plate; 11. Threaded column; 12. Positioning sleeve; 13. Socket; 14. Slide opening; 15. Slide block; 16. Pressure plate; 17. Pressure foot; 18. Lower guide plate; 19. Lifting plate; 20. Limiting teeth; 21. Top connecting plate; 22. Reed; 23. Lifting column; 24. Pulling plate; 25. Limiting tooth groove; 26. Connecting rod; 27. Wire passing plate; 28. Needle handle; 29. Needle tip; 30. Connecting pipe; 31. Sleeve; 32. Guide rail; 33. Guide block; 34. Guide rod; 35. Injection mechanism; 36. Detection mechanism; 37. Host module. Detailed implementation manner

[0027] The following description is used to disclose the present invention so that those skilled in the art can implement the present invention. The preferred embodiments in the following description are only examples, and those skilled in the art can think of other obvious variations.

[0028] As Figures 1 - 7 shown, a glucose clamp test device based on a negative feedback model includes a frustum 1. A test chamber 2 is fixedly installed at the front edge of the upper surface of the frustum 1. Chamber doors 3 are respectively rotatably installed on both sides of the front end of the test chamber 2. A fixed clamping plate 4 is connected inside the test chamber 2 through an XY-axis moving mechanism. An upper connecting plate 9 and a lower connecting plate 8 are respectively fixedly connected to the upper and lower ends of the fixed clamping plate 4. A movable clamping plate 5 is connected to one side of the fixed clamping plate 4 through a plugging mechanism. A slide opening 14 is penetrated and opened on the upper surface of the upper connecting plate 9. Two slide blocks 15 are slidably inserted inside the slide opening 14 through an elastic meshing mechanism. A pressure plate 16 is fixedly connected to the lower end of the slide block 15. Pressure feet 17 are respectively fixedly connected to both sides of the lower surface of the pressure plate 16. Needle tips 29 are respectively arranged below every two pressure feet 17. A needle handle 28 is fixedly arranged on the outer surface of the needle tip 29.

[0029] As Figure 2 、 Figure 3 shown, the XY-axis moving mechanism includes guide rails 32 fixedly connected to the front and rear edges of the upper surface of the test chamber 2 respectively. Guide blocks 33 are slidably clamped inside the guide rails 32. Two guide rods 34 are fixedly connected between the front and rear guide blocks 33. A connecting rod 26 is fixedly connected to the outer surface of the fixed clamping plate 4. Two sleeves 31 are fixedly embedded at the upper end of the connecting rod 26. The sleeves 31 are slidably sleeved on the outer surface of the connecting rod 26. When the white rat positioned between the movable clamping plate 5 and the fixed clamping plate 4 moves, the sleeve 31 will slide back and forth along the surface of the guide rod 34, and the guide block 33 will slide horizontally along the guide rail 32. Therefore, even after positioning, the movement of the white rat will not be completely restricted, preventing the acting force when the white rat moves from directly acting on the movable clamping plate 5 and the fixed clamping plate 4 above, thus causing problems such as positioning loosening or structural damage.

[0030] As Figure 7 shown, a connecting pipe 30 is fixedly connected to the upper end of the needle 29, a wire passing plate 27 extending rearward is fixedly connected to the upper surface of the connecting rod 26, and the connecting pipe 30 penetrates through the outer surface of the wire passing plate 27. The wire passing plate 27 is made of hard rubber material to ensure that after the connecting pipe 30 passes through, the frictional force at the contact position with the wire passing plate 27 can keep the connecting pipe 30 stable and will not slide relative to the wire passing plate 27 under gravity.

[0031] As Figure 1 shown, an injection mechanism 35 is connected to the end of one of the connecting pipes 30, a detection mechanism 36 is connected to the end of the other connecting pipe 30, the injection mechanism 35 and the detection mechanism 36 are respectively fixedly installed on the upper surface of the table body 1, and a host module 37 is fixedly installed on the upper surface of the table body 1. The host module 37 is used to control the injection mechanism 35 and the detection mechanism 36. The injection mechanism 35 is used to inject insulin, the detection mechanism 36 is used to aspirate the connecting pipe 30 to obtain a blood sample for blood glucose detection, and the data is transmitted to the host module 37.

[0032] As Figure 3 , Figure 4 , Figure 5 shown, the elastic meshing mechanism includes lower guide plates 18 respectively fixedly connected to the front and rear surfaces of the slider 15. The upper surface of the lower guide plate 18 is slidably attached to the lower surface of the upper connecting plate 9. An elevating plate 19 is slidably sleeved on the outer surface of the slider 15 through an elastic mechanism. A plurality of limiting teeth 20 are fixedly connected to the lower surface of the elevating plate 19. A limiting tooth groove 25 is opened on the upper surface of the upper connecting plate 9. The limiting teeth 20 are engaged into the inner side of the limiting tooth groove 25 to achieve meshing and position the slider 15.

[0033] The elastic mechanism includes a top connecting plate 21 fixedly connected to the upper end of the slider 15. A reed 22 is fixedly connected to the lower surface of the top connecting plate 21. The lower end of the reed 22 is fixedly connected to the upper surface of the elevating plate 19. A plurality of elevating columns 23 are fixedly connected to the upper surface of the elevating plate 19. The elevating columns 23 slidably pass through the lower surface of the top connecting plate 21. The upper ends of the plurality of elevating columns 23 are commonly fixedly connected to a pulling plate 24, and the pulling plate 24 facilitates the user to lift the elevating plate 19.

[0034] As Figure 6 shown, a splicing plate 10 is fixedly connected to the lower end of the movable clamping plate 5. An insertion opening 13 is penetrated and opened at the edge of the lower surface of the splicing plate 10. A threaded column 11 is fixedly connected to the lower surface of the lower connecting plate 8. The threaded column 11 slidably passes through the inner side of the insertion opening 13. A positioning sleeve 12 is threadedly sleeved on the lower end of the threaded column 11. The upper surface of the positioning sleeve 12 contacts the lower surface of the splicing plate 10. By rotating, the positioning sleeve 12 can be removed, and the movable clamping plate 5 and the fixed clamping plate 4 can be separated.

[0035] AsFigure 6 As shown, a first cushion pad 6 is fixedly connected to the inner wall of the fixed clamping plate 4 and the upper surface of the lower connecting plate 8, and a second cushion pad 7 is fixedly connected to the inner wall of the movable clamping plate 5. Both the first cushion pad 6 and the second cushion pad 7 are made of memory cotton material to make the positioning of the white rat more stable.

[0036] The user places the white rat with its back downwards, inserts two needles 29 into the carotid artery of the white rat, and after placing it inside the fixed clamping plate 4, closes the movable clamping plate 5. During this process, the threaded column 11 slides relatively along the inner side of the socket 13. After sliding in, the positioning sleeve 12 is screwed on so that the upper end of the positioning sleeve 12 tightly abuts against the lower surface of the splicing plate 10 to complete the limit. At this time, the white rat is positioned between the movable clamping plate 5 and the fixed clamping plate 4. Then, the pulling plate 24 is lifted upwards so that the limiting teeth 20 are disengaged from the inner side of the limiting tooth groove 25, and then the slider 15 is moved horizontally until the two pressing feet 17 on each side respectively press on the surface of the needle handle 28. After releasing the hand, under the elastic force of the reed 22, the pressing feet 17 stably abut against the needle handle 28 to prevent the needle 29 from loosening. It has the function of positioning the white rat, and there is no need to anesthetize or tie the white rat during the experiment, which is convenient to operate, and the inserted needle 29 can be firmly positioned to prevent the problem of detachment and loosening.

[0037] When arranging the white rat, the connecting pipe 30 is pulled upwards relative to the wire passing plate 27 so that the connecting pipe 30 will not have too much redundancy near the bottom of the test box 2. At the same time, due to the clamping effect of the fixed clamping plate 4 and the movable clamping plate 5, the movement range of the front and hind legs of the white rat can be effectively restricted. Generally speaking, the problem that the hind legs of the white rat touch the connecting pipe 30 can be effectively prevented.

[0038] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art of this industry should understand that the present invention is not limited by the above embodiments. What is described in the above embodiments and the specification is only the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements all fall within the scope of the present invention claimed. The scope of protection claimed by the present invention is defined by the appended claims and their equivalents.

Claims

1. A glucose clamp test device based on a negative feedback model, comprising a platform (1), characterized in that: A test box (2) is fixedly installed at the front edge of the upper surface of the platform (1), and box doors (3) are rotatably installed on both sides of the front end of the test box (2). The inner side of the test box (2) is connected to a fixed clamping plate (4) through an XY axis moving mechanism, and the upper and lower ends of the fixed clamping plate (4) are respectively fixedly connected to an upper connecting plate (9) and a lower connecting plate (8), and one side of the fixed clamping plate (4) is connected to a movable clamping plate (5) through a plug-in mechanism. A sliding opening (14) is penetrated through the upper surface of the upper connecting plate (9), and two sliders (15) are slidably inserted into the inner side of the sliding opening (14) through an elastic meshing mechanism. The lower end of the slider (15) is fixedly connected to a pressing plate (16), and the two sides of the lower surface of the pressing plate (16) are respectively fixedly connected to presser feet (17), and needles (29) are respectively arranged below each of the two presser feet (17), and needle handles (28) are fixedly arranged on the outer surface of the needles (29); The XY axis moving mechanism comprises a guide rail (32) fixedly connected to the front and rear edges of the upper surface of the test box (2); a guide block (33) is slidably mounted on the inner side of the guide rail (32); two guide rods (34) are fixedly connected between the front and rear guide blocks (33); a connecting rod (26) is fixedly connected to the outer surface of the fixed clamping plate (4); two sleeves (31) are fixedly embedded at the upper end of the connecting rod (26); the sleeves (31) are slidably mounted on the outer surface of the guide rod (34); The elastic meshing mechanism comprises a lower guide plate (18) respectively fixedly connected to the front and rear surfaces of the slider (15), the upper surface of the lower guide plate (18) slidingly contacts the lower surface of the upper connecting plate (9), the outer surface of the slider (15) is provided with a lifting plate (19) slidingly sleeved by an elastic mechanism, the lower surface of the lifting plate (19) is fixedly connected to a plurality of limiting teeth (20), the upper surface of the upper connecting plate (9) is provided with a limiting tooth groove (25), and the limiting teeth (20) are snapped into the inner side of the limiting tooth groove (25); The elastic mechanism comprises a top-connecting plate (21) fixedly connected to the upper end of the slider (15); a spring sheet (22) is fixedly connected to the lower surface of the top-connecting plate (21); the lower end of the spring sheet (22) is fixedly connected to the upper surface of the lifting plate (19); a plurality of lifting columns (23) are fixedly connected to the upper surface of the lifting plate (19); the lifting columns (23) slide through the lower surface of the top-connecting plate (21); and the upper ends of the plurality of lifting columns (23) are commonly fixedly connected to a pulling plate (24).

2. The glucose clamp test device based on the negative feedback model according to claim 1, characterized in that: The upper end of the needle (29) is fixedly connected to a connecting tube (30), the upper surface of the connecting rod (26) is fixedly connected to a wire-passing plate (27) extending toward the rear, and the connecting tube (30) passes through the outer surface of the wire-passing plate (27).

3. A glucose clamp test device based on a negative feedback model according to claim 2, characterized in that: The end of one of the connecting tubes (30) is connected to an injection mechanism (35), and the end of the other connecting tube (30) is connected to a detection mechanism (36); the injection mechanism (35) and the detection mechanism (36) are respectively fixedly mounted on the upper surface of the platform (1); and a host module (37) is fixedly mounted on the upper surface of the platform (1).

4. The glucose clamp test device based on the negative feedback model according to claim 1, characterized in that: The lower end of the movable clamping plate (5) is fixedly connected to a splicing plate (10), and a socket (13) is formed through the edge of the lower surface of the splicing plate (10). The lower surface of the lower connecting plate (8) is fixedly connected to a threaded column (11), and the threaded column (11) slides through the inner side of the socket (13). The lower end of the threaded column (11) is threadedly sleeved with a positioning sleeve (12), and the upper surface of the positioning sleeve (12) contacts the lower surface of the splicing plate (10).

5. The glucose clamp test device based on the negative feedback model according to claim 1, characterized in that: The inner wall of the fixed clamping plate (4) and the upper surface of the lower connecting plate (8) are jointly fixedly connected with a first protective pad (6), and the inner wall of the movable clamping plate (5) is fixedly connected with a second protective pad (7).

Images