A soothing, sunscreen spray and methods of making and using the same

Abstract

The present application relates to the field of cosmetic technology, and in particular to a soothing sunscreen spray, a preparation method and application thereof.The spray raw material comprises cyclopentasiloxane, octyl trimethylsiloxysilicate, polydimethylsiloxane, butylene glycol, polymethylsilsesquioxane, ethylhexyl salicylate, diethylhexyl aminobenzozyol hexyl benzoate, octocrylene, lauryl peg-10 tri(trimethylsiloxy) silyethyl dimethicone, ethylhexyl triazone, gynostemma pentaphyllum extract, gentiana scabra extract and water.The spray prepared by the present application has excellent skin soothing, sunscreen and skin non-dull effects.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to a soothing sunscreen spray and a preparation method and application thereof. BACKGROUND

[0002] The main function of sunscreen cosmetics is to block ultraviolet rays and protect the skin from ultraviolet damage. Ultraviolet rays can cause a variety of damage to the skin, including tanning, sunburn, and aging. Sunscreen cosmetics absorb, reflect, or scatter ultraviolet rays, reducing the direct irradiation of ultraviolet rays on the skin, thereby protecting the skin from these damages.

[0003] The ingredients of sunscreen cosmetics mainly include chemical sunscreens and physical sunscreens. Chemical sunscreens absorb ultraviolet rays and convert them into heat release, while physical sunscreens protect the skin by reflecting and scattering ultraviolet rays. In addition, some sunscreen products also contain antioxidants and anti-inflammatory ingredients, which help to alleviate the inflammatory response and pigmentation caused by ultraviolet rays.

[0004] Different types of sunscreen cosmetics are suitable for different skin types and use scenarios. Chemical sunscreens are usually suitable for use in environments with strong ultraviolet rays, while physical sunscreens are suitable for sensitive skin due to their safety and stability.

[0005] At the same time, due to the use of multiple chemical ingredients in sunscreen cosmetics, it is easy to cause skin allergies or make the skin uncomfortable. At the same time, the sunscreen strength is uneven. Therefore, it is a technical problem to be solved to provide a soothing sunscreen spray. SUMMARY

[0006] The present application is directed to the above technical problems, and the technical solution of the present application is provided, which includes a soothing sunscreen spray and a preparation method and application thereof.

[0007] In one aspect, the present application provides a soothing sunscreen spray, the raw materials of which include cyclopentasiloxane, octyl trimethylsiloxysilicate, polydimethylsiloxane, butylene glycol, polymethylsilsesquioxane, ethylhexyl salicylate, diethylamino hydroxybenzoyl hexyl benzoate, octocrylene, lauryl PEG-10 tris (trimethylsiloxy) methylsilyl ethyl dimethicone, ethylhexyl triazone, and water.

[0008] Preferably, the soothing, sunscreen spray comprises, by mass percentage, 1-5% cyclopentasiloxane, 0.5-3% octyl trimethylsiloxysilicate, 0.5-2% dimethicone, 8-12% butylene glycol, 0.5-2% polymethylsilsesquioxane, 0.4-1.5% ethylhexyl salicylate, 0.3-1.5% diethylhexyl 2,6-piperidinedicarboxylic acid hexyl ester, 0.3-1.5% octocrylene, 4-8% lauryl PEG-10 tri(trimethylsiloxy) silylethyl dimethicone, 0.4-1.5% ethylhexyl triazone, and the balance water.

[0009] Preferably, the mass ratio of butylene glycol to lauryl PEG-10 tri(trimethylsiloxy) silylethyl dimethicone in the soothing, sunscreen spray is 1.5-2.2:1.

[0010] Preferably, the soothing, sunscreen spray further comprises 0.5-2% Centella asiatica extract and 0.5-1.8% Gentiana scabra extract.

[0011] Preferably, the Centella asiatica extract suitable for the soothing, sunscreen spray of the present application is prepared by a process comprising enzymatic hydrolysis with bromelain and fermentation with a fermentation bacterium. The extract prepared by the process has better effects than the extract prepared by traditional alcohol extraction, water extraction or extraction process, and can better exert the pharmacological effects of the active ingredients of Centella asiatica.

[0012] Preferably, the Gentiana scabra extract suitable for the soothing, sunscreen spray of the present application is prepared by a process comprising extraction with an ethanol solution having a mass concentration of 40-55% and ultrafiltration. The process can effectively promote the extraction and exertion of the active ingredients of Gentiana scabra roots.

[0013] Preferably, the mass ratio of the Centella asiatica extract to the Gentiana scabra extract is 0.8-1:1.

[0014] Preferably, the preparation method of the Centella asiatica extract comprises:

[0015] Step 1: crushing dried whole plants of Centella asiatica, heating and keeping warm with water to obtain product 1;

[0016] Step 2: sterilizing product 1, adding bromelain, glucose, dipotassium hydrogen phosphate and a fermentation bacterium, fermenting, sterilizing to obtain product 2;

[0017] Step 3: filtering product 2 to obtain a filtrate, drying to obtain the Centella asiatica extract.

[0018] Preferably, the mass of water is 10-30 times that of the whole plants of Centella asiatica.

[0019] Preferably, the heating and incubation conditions are: heating to 60-80℃, and incubating for 2-10 hours.

[0020] Preferably, the bromelain is added in an amount of 600-1500 U / g of product 1.

[0021] Preferably, the glucose is added in an amount of 1-4% of the mass of product 1.

[0022] Preferably, the dipotassium hydrogen phosphate is added in an amount of 0.02-0.1% of the mass of product 1.

[0023] Preferably, the fermentation bacteria are Lactobacillus reuteri CICC 6122 and Lactobacillus plantarum CICC 20322, and the ratio of the two is 1:0.4-0.8.

[0024] Preferably, the amount of the fermentation bacteria is 1×10 9 -9×10 9 cfu / g of product 1.

[0025] Preferably, the fermentation temperature is 30-45℃, and the time is 65-150 hours.

[0026] Preferably, the preparation method of the gentian extract comprises:

[0027] (1) crushing dried gentian roots, adding 8-40 times the mass of the gentian roots of an ethanol aqueous solution, incubating and stirring at 50-65℃ for 3-10 hours to obtain an intermediate product 1;

[0028] (2) filtering the intermediate product 1, ultrafiltrating, then concentrating under reduced pressure to 0.05-0.12 times the mass of the intermediate product, and drying to obtain the gentian extract.

[0029] Preferably, the mass concentration of the ethanol aqueous solution is 40-55%.

[0030] Preferably, the ultrafiltration is performed using an ultrafiltration membrane with a molecular weight cut-off of less than 800 Da.

[0031] The application also provides a preparation method of the soothing and sunscreen spray, comprising the following steps: adding cyclopentasiloxane, octyl trimethylsiloxysilicate, polydimethylsiloxane, butylene glycol, polymethylsilsesquioxane, ethylhexyl salicylate, diethylhexyl aminobenzozyol hydrobenzoate, octocrylene, lauryl PEG-10 tri (trimethylsiloxy) methylsiloxyethyl dimethicone and ethylhexyl triazone into a container, heating to 60-85℃, and stirring to mix; then adding water and stirring; cooling, then adding extract of Centella asiatica and gentian extract, stirring to mix, sterilizing, and obtaining the soothing and sunscreen spray.

[0032] Preferably, the above-mentioned spray can be added to a spray container to form a cosmetic product. Alternatively, a propellant can be added as needed to obtain a spray product.

[0033] Another aspect of the present application also provides the use of the soothing, sunscreen spray in the non-therapeutic use of cosmetics.

[0034] Advantages of the present application:

[0035] The present application uses cyclotetrasiloxane, octylpolymethylsiloxane, polydimethylsiloxane, polymethylsilsesquioxane of emollient ingredients, ethylhexyl salicylate, diethylamino hydroxybenzoyl hexyl benzoate, octocrylene of sunscreen ingredients, to give the spray excellent skin moisturizing and sunscreen effects. At the same time, lauryl PEG-10 tri (trimethylsiloxy) silyl ethyl polydimethylsiloxane and butylene glycol are added, the ratio of the two is controlled, and the spray is given better stability and use effect. At the same time, the active ingredients of the extract of centella asiatica and gentian root are added to give the spray soothing skin and make the skin not dark, and the extraction process of the extract is further optimized to further improve the anti-inflammatory effect of the spray.

[0036] The spray prepared by the present application has excellent soothing skin, sunscreen and skin lightening effects.

[0037] The centella asiatica extract and gentian extract prepared by the present application have excellent effects of reducing the expression amount of IL-6 and TNF-α, can effectively inhibit inflammation, and have good soothing skin effect.

[0038] The addition of bromelain during the fermentation of centella asiatica can effectively promote the improvement of the expression amount of IL-6 and TNF-α of the extract. During fermentation, the use of a combination of Lactobacillus reuteri CICC 6122 and Lactobacillus plantarum CICC 20322 as fermentation bacteria can effectively improve the anti-inflammatory effect. The combination of the two can synergistically improve the effect of inhibiting the expression of IL-6 and TNF-α. The co-fermentation of the two can promote the improvement of the richness of soothing active ingredients and effectively improve the soothing effect of the extract after concentration and other treatments. When the ratio of the two is 1:0.4-0.8, the effect is better.

[0039] During the extraction of gentian root, 40-55% ethanol aqueous solution is used for extraction and is combined with a low molecular weight ultrafiltration membrane with a molecular weight cut-off of less than 800 Da for interception treatment, which can effectively improve the soothing effect of the extract and give the extract better use efficiency.

[0040] The Centella asiatica extract and the gentian extract are matched, and a mutual promotion relationship is generated within a certain proportion range, and an antagonistic effect is generated in other proportion ranges. When a matched product with excellent soothing performance is selected, the Centella asiatica extract and the gentian extract have the best effect when the mass ratio is 0.8-1:1.

[0041] The SPF value of the prepared spray sunscreen reaches 47.6, the refreshing and non-greasy score reaches 9.6, the spray is not layered and no precipitation is generated after being placed at 55 DEG C for one week; the spray has excellent sunscreen effect, is refreshing and non-greasy after being applied, and has good temperature stability.

[0042] In the preparation system, when the dosage ratio of butylene glycol and PEG-10 tri(trimethylsiloxy)silyl ethyl dimethicone is within the range of 1.5-2.2:1, the product has better effect.

[0043] The spray preparation method is simple, raw materials are convenient to obtain, the spray has excellent effect, and has good application prospect. DETAILED DESCRIPTION

[0044] The application will be described below in conjunction with specific embodiments, and various effects of the application will be more clearly presented. Those skilled in the art should understand that the specific embodiments are used to illustrate the application, rather than limit the application.

[0045] Lactobacillus reuteri CICC 6122 and Lactobacillus plantarum CICC 20322 are purchased from the China Industrial Microbial Culture Collection Center. Cyclopentasiloxane is purchased from Jinan Yuno Chemical Co., Ltd. Octyl trimethylsiloxysilicate is purchased from Shandong Suihua Biological Technology Co., Ltd. Dimethicone is purchased from Hubei Xinghengye Technology Co., Ltd. Polymethylsilsesquioxane is purchased from Shanghai Maier Biochemical Technology Co., Ltd. Octocrylene is purchased from Shandong Mao Yuan Chemical Co., Ltd.

[0046] I. Preparation and testing of the extract

[0047] A. Preparation of the Centella asiatica extract

[0048] Centella asiatica extract 1:

[0049] The preparation method is as follows: step 1: dry Centella asiatica whole plant is crushed to a particle size of less than 80 mesh, 18 times the mass of the whole plant of water is added, heated to 75 DEG C and kept at 60 rpm for 8.5 hours, and product 1 is obtained;

[0050] Step 2: product 1 is sterilized, cooled to room temperature, bromelain, glucose, dipotassium hydrogen phosphate and fermentation bacteria are added, anaerobic fermentation is carried out at 35 DEG C for 135 hours, sterilization is carried out, and product 2 is obtained;

[0051] Step 3: The product 2 was filtered by using a filter membrane with a pore size of 0.2 microns to obtain a filtrate, and the filtrate was freeze-dried at -40℃ until the water content was 2.67wt% to obtain the extract of the Gotthola Relamis.

[0052] The bromelain was added in an amount of 1200 U / g of the product 1; the glucose was added in an amount of 3.02% of the mass of the product 1; and the dipotassium hydrogen phosphate was added in an amount of 0.065% of the mass of the product 1.

[0053] The fermentation bacteria were Lactobacillus reuteri CICC 6122 and Lactobacillus plantarum CICC 20322, and the quantity ratio was 1:0.6; the fermentation bacteria were used in an amount of 4.80 x 10 9 cfu / g of the product 1.

[0054] The extract of the Gotthola Relamis 2:

[0055] The preparation method was as follows: Step 1: The dried Gotthola Relamis whole plant was crushed to a particle size of less than 80 mesh, 20 times the mass of the Gotthola Relamis whole plant was added, heated to 72℃ and kept at 60 rpm for 9 hours, and product 1 was obtained;

[0056] Step 2: The product 1 was sterilized, cooled to room temperature, and the bromelain, glucose, dipotassium hydrogen phosphate and fermentation bacteria were added, and anaerobic fermentation was carried out at 36℃ for 120 hours, and sterilization was carried out to obtain product 2;

[0057] Step 3: The product 2 was filtered by using a filter membrane with a pore size of 0.2 microns to obtain a filtrate, and the filtrate was freeze-dried at -40℃ until the water content was 2.71wt% to obtain the extract of the Gotthola Relamis.

[0058] The bromelain was added in an amount of 1000 U / g of the product 1; the glucose was added in an amount of 3.28% of the mass of the product 1; and the dipotassium hydrogen phosphate was added in an amount of 0.07% of the mass of the product 1.

[0059] The fermentation bacteria were Lactobacillus reuteri CICC 6122 and Lactobacillus plantarum CICC 20322, and the quantity ratio was 1:0.4; the fermentation bacteria were used in an amount of 4.92 x 10 9 cfu / g of the product 1.

[0060] The extract of the Gotthola Relamis 3:

[0061] The preparation method was as follows: Step 1: The dried Gotthola Relamis whole plant was crushed to a particle size of less than 80 mesh, 20 times the mass of the Gotthola Relamis whole plant was added, heated to 72℃ and kept at 60 rpm for 9 hours, and product 1 was obtained;

[0062] Step 2: The product 1 was sterilized, cooled to room temperature, and the bromelain, glucose, dipotassium hydrogen phosphate and fermentation bacteria were added, and anaerobic fermentation was carried out at 36℃ for 120 hours, and sterilization was carried out to obtain product 2;

[0063] Step 3: The product 2 was filtered by a filter membrane with a pore size of 0.2 microns to obtain a filtrate, and the filtrate was freeze-dried at -40℃ until the water content was 2.67wt% to obtain the extract of the Centella asiatica.

[0064] The added amount of glucose was 3.02% of the mass of the product 1; the added amount of dipotassium hydrogen phosphate was 0.065% of the mass of the product 1.

[0065] The fermentation bacteria were Lactobacillus reuteri CICC 6122 and Lactobacillus plantarum CICC 20322, and the quantity ratio was 1:0.6; the amount of the fermentation bacteria was 4.80×10 9 cfu / g of the product 1.

[0066] The extract of the Centella asiatica 4:

[0067] The preparation method was as follows: Step 1: dry Centella asiatica whole plants were crushed to a particle size of less than 80 mesh, 18 times the mass of the Centella asiatica whole plants was added with water, heated to 75℃ and kept at 75℃ and stirred at 60 rpm for 8.5 hours to obtain product 1;

[0068] Step 2: the product 1 was sterilized, cooled to room temperature, bromelain, glucose, dipotassium hydrogen phosphate and fermentation bacteria were added, and anaerobic fermentation was carried out at 35℃ for 135 hours, and then sterilized to obtain product 2;

[0069] Step 3: the product 2 was filtered by a filter membrane with a pore size of 0.2 microns to obtain a filtrate, and the filtrate was freeze-dried at -40℃ until the water content was 2.67wt% to obtain the extract of the Centella asiatica.

[0070] The added amount of glucose was 3.02% of the mass of the product 1; the added amount of dipotassium hydrogen phosphate was 0.065% of the mass of the product 1.

[0071] The fermentation bacteria were Lactobacillus reuteri CICC 6122; the amount of the fermentation bacteria was 4.80×10 9 cfu / g of the product 1.

[0072] The extract of the Centella asiatica 5:

[0073] The preparation method was as follows: Step 1: dry Centella asiatica whole plants were crushed to a particle size of less than 80 mesh, 18 times the mass of the Centella asiatica whole plants was added with water, heated to 75℃ and kept at 75℃ and stirred at 60 rpm for 8.5 hours to obtain product 1;

[0074] Step 2: the product 1 was sterilized, cooled to room temperature, bromelain, glucose, dipotassium hydrogen phosphate and fermentation bacteria were added, and anaerobic fermentation was carried out at 35℃ for 135 hours, and then sterilized to obtain product 2;

[0075] Step 3: The product 2 is filtered by using a filter membrane with a pore size of 0.2 microns to obtain a filtrate, and the filtrate is freeze-dried at -40℃ until the water content is 2.67wt% to obtain the extract of G. pentaphyllum.

[0076] The bromelain is added in an amount of 1200U / g of the product 1; the glucose is added in an amount of 3.02% of the mass of the product 1; and the dipotassium hydrogen phosphate is added in an amount of 0.065% of the mass of the product 1.

[0077] The fermenting bacteria are Lactobacillus reuteri CICC 6122 and Lactobacillus plantarum CICC 20322 in a number ratio of 1:0.1; and the fermenting bacteria are used in an amount of 4.80x10 9 cfu / g of the product 1.

[0078] The extract of G. pentaphyllum 6 is prepared by the following method:

[0079] Step 1: The dried G. pentaphyllum whole plant is crushed to a particle size of less than 80 mesh, 18 times the mass of the G. pentaphyllum whole plant is added, heated to 75℃ and kept at this temperature, and stirred at 60rpm for 8.5 hours to obtain product 1;

[0080] Step 2: The product 1 is sterilized, cooled to room temperature, and then the bromelain, glucose, dipotassium hydrogen phosphate and fermenting bacteria are added, and anaerobic fermentation is carried out at 35℃ for 135 hours, and then sterilized to obtain product 2;

[0081] Step 3: The product 2 is filtered by using a filter membrane with a pore size of 0.2 microns to obtain a filtrate, and the filtrate is freeze-dried at -40℃ until the water content is 2.67wt% to obtain the extract of G. pentaphyllum.

[0082] The bromelain is added in an amount of 1200U / g of the product 1; the glucose is added in an amount of 3.02% of the mass of the product 1; and the dipotassium hydrogen phosphate is added in an amount of 0.065% of the mass of the product 1.

[0083] The fermenting bacteria are Lactobacillus reuteri CICC 6122 and Lactobacillus plantarum CICC 20322 in a number ratio of 1:0.1; and the fermenting bacteria are used in an amount of 4.80x10 9 cfu / g of the product 1.

[0084] The extract of G. pentaphyllum 7 is prepared by the following method:

[0085] Step 1: The dried G. pentaphyllum whole plant is crushed to a particle size of less than 80 mesh, 18 times the mass of the G. pentaphyllum whole plant is added, heated to 75℃ and kept at this temperature, and stirred at 60rpm for 8.5 hours to obtain product 1;

[0086] Step 2: The product 1 is sterilized, cooled to room temperature, and then the bromelain, glucose, dipotassium hydrogen phosphate and fermenting bacteria are added, and anaerobic fermentation is carried out at 35℃ for 135 hours, and then sterilized to obtain product 2;

[0087] Step 3: The product 2 was filtered by using a filter membrane with a pore size of 0.2 microns to obtain a filtrate, and the filtrate was freeze-dried at -40℃ until the water content was 2.67wt% to obtain the extract of Gynostemma pentaphyllum.

[0088] The bromelain was added in an amount of 1200 U / g of product 1; the glucose was added in an amount of 3.02% of the mass of product 1; and the potassium phosphate dibasic was added in an amount of 0.065% of the mass of product 1.

[0089] The fermentation bacteria were Lactobacillus reuteri CICC 6122 and Lactobacillus plantarum CICC 20322, and the ratio of the two bacteria was 1:2; the amount of the fermentation bacteria was 4.80 x 10 9 cfu / g of product 1.

[0090] Preparation of the extract of Gynostemma pentaphyllum

[0091] The extract of Gynostemma pentaphyllum 1:

[0092] The preparation method was as follows: (1) dry Gynostemma pentaphyllum roots were crushed, 30 times the mass of the Gynostemma pentaphyllum roots and an ethanol aqueous solution with a mass concentration of 45% were added, 60℃ incubation and 70 rpm stirring for 7.5 hours to obtain intermediate product 1;

[0093] (2) intermediate product 1 was filtered by using a filter membrane with a pore size of 1 micrometer, the filtrate was ultrafiltered by using an ultrafiltration membrane with a molecular weight cut-off of 500 Da, then the ultrafiltrate was concentrated at 55℃ under reduced pressure to 0.1 times the mass of intermediate product 1, and freeze-dried until the water content was 2.91wt% to obtain the extract of Gynostemma pentaphyllum.

[0094] The extract of Gynostemma pentaphyllum 2:

[0095] The preparation method was as follows: (1) dry Gynostemma pentaphyllum roots were crushed, 32 times the mass of the Gynostemma pentaphyllum roots and an ethanol aqueous solution with a mass concentration of 52% were added, 60℃ incubation and 70 rpm stirring for 7.2 hours to obtain intermediate product 1;

[0096] (2) intermediate product 1 was filtered by using a filter membrane with a pore size of 1 micrometer, the filtrate was ultrafiltered by using an ultrafiltration membrane with a molecular weight cut-off of 500 Da, then the ultrafiltrate was concentrated at 55℃ under reduced pressure to 0.105 times the mass of intermediate product 1, and freeze-dried until the water content was 2.82wt% to obtain the extract of Gynostemma pentaphyllum.

[0097] The extract of Gynostemma pentaphyllum 3:

[0098] The preparation method was as follows: (1) dry Gynostemma pentaphyllum roots were crushed, 30 times the mass of the Gynostemma pentaphyllum roots and an ethanol aqueous solution with a mass concentration of 20% were added, 60℃ incubation and 70 rpm stirring for 7.5 hours to obtain intermediate product 1;

[0099] (2), the intermediate product 1 is filtered by using a filter membrane with a pore size of 1 micron, the filtrate is ultrafiltered by using an ultrafiltration membrane with a molecular weight cut-off of 500 Da, then the ultrafiltrate is concentrated to 0.1 times the mass of the intermediate product 1 at 55°C under reduced pressure, and freeze-dried to a water content of 2.91wt%, to obtain the gentian extract.

[0100] Gentian extract 4:

[0101] The preparation method is: (1) the dried gentian root is crushed, 30 times the mass of the gentian root and an ethanol aqueous solution with a mass concentration of 70% are added, 60°C is kept and stirred at 70 rpm for 7.5 hours, to obtain the intermediate product 1;

[0102] (2), the intermediate product 1 is filtered by using a filter membrane with a pore size of 1 micron, the filtrate is ultrafiltered by using an ultrafiltration membrane with a molecular weight cut-off of 500 Da, then the ultrafiltrate is concentrated to 0.1 times the mass of the intermediate product 1 at 55°C under reduced pressure, and freeze-dried to a water content of 2.91wt%, to obtain the gentian extract.

[0103] Gentian extract 5:

[0104] The preparation method is: (1) the dried gentian root is crushed, 30 times the mass of the gentian root and an ethanol aqueous solution with a mass concentration of 45% are added, 60°C is kept and stirred at 70 rpm for 7.5 hours, to obtain the intermediate product 1;

[0105] (2), the intermediate product 1 is filtered by using a filter membrane with a pore size of 1 micron, the filtrate is ultrafiltered by using an ultrafiltration membrane with a molecular weight cut-off of 500 Da, then the ultrafiltrate is concentrated to 0.1 times the mass of the intermediate product 1 at 55°C under reduced pressure, and freeze-dried to a water content of 2.91wt%, to obtain the gentian extract.

[0106] C, the extract inhibits inflammation and relieves skin effect performance test

[0107] RAW264.7 cells are inoculated into a 24-well cell culture plate at a density of 1.2×10 5 Each well contains 0.5mL. After 24h of culture, LPS is added to the cells in each group except the blank control group to a concentration of 1μg / mL to establish a cell inflammation model. In the model group, only LPS is added to the cells to a concentration of 1μg / mL. In each experimental group, LPS is added to the cells to a concentration of 1μg / mL and each extract diluent is added to a concentration of 0.01% (the extract diluent is prepared by diluting the extract with deionized water to a mass concentration of 1%). Each group is incubated at 37°C for 24h. Then the upper culture solution is collected, and the expression amounts of IL-6 and TNF-α in each group are detected according to the instructions of the ELISA kit. Each group is tested in parallel for 5 times to obtain the average value. The results are shown in Table 1.

[0108] Table 1: Expression amount of IL-6 and TNF-α of each group of extract

[0109]

[0110]

[0111] According to the test in Table 1, the extract of radix rubus chingii and the extract of gentiana prepared by the present application have excellent effect of reducing the expression amount of IL-6 and TNF-α, can effectively inhibit inflammation, and have good skin soothing effect.

[0112] According to the test of extract of radix rubus chingii 1 and 3, it is known that the addition of bromelain in the fermentation process can effectively promote the improvement of the extract in inhibiting the expression amount of IL-6 and TNF-α. According to the test of extract 1, 4-7, it is known that the combination of Lactobacillus reuteri CICC 6122 and Lactobacillus plantarum CICC 20322 can effectively improve the effect of inhibiting inflammation, the combination of the two can synergistically improve the effect of inhibiting the expression amount of IL-6 and TNF-α, and the combination of the two can promote the improvement of the richness of soothing active ingredients and effectively improve the soothing effect of the extract after concentration and other treatments; and when the ratio of the two is 1:0.4-0.8, the effect is better.

[0113] According to the test of extract of gentiana 1-5, it is known that the use of 40-55% ethanol aqueous solution for extraction and the combination with ultrafiltration membrane with a molecular weight cut-off lower than 800 Da can effectively improve the soothing effect of the extract and give the extract better use efficiency.

[0114] D. Test of effect of combination of extracts

[0115] The extract of radix rubus chingii 1 and the extract of gentiana 1 are combined and tested. The test method is carried out according to C. And the original test records "each experimental group is added with LPS to a concentration of 1 μg / mL and each extract diluent is added to a concentration of 0.01% (wherein the extract diluent is diluted with deionized water to a mass concentration of 1%) " is replaced with "each experimental group is added with LPS to a concentration of 1 μg / mL and each extract combination diluent is added to a concentration of 0.01% (wherein the extract combination diluent is diluted with deionized water to a mass concentration of 1%) ", and the others remain unchanged. The specific combination ratio and test results are shown in Table 2. Among them, C represents the combination of different mass ratios of extract of radix rubus chingii 1 and extract of gentiana 1.

[0116] Table 2: Expression amount of IL-6 and TNF-α of each group of extract combination

[0117]

[0118]

[0119] According to the test in Table 2, when the Centella asiatica extract 1 and the Gentiana scabra extract 1 are combined, they have a mutual promotion relationship within a certain ratio range, and an antagonistic effect in other ratio ranges. Therefore, when selecting a combination with excellent soothing performance, the Centella asiatica extract and the Gentiana scabra extract have the best effect when the mass ratio is 0.8-1:1.

[0120] II. Preparation and testing of cosmetic sprays

[0121] 1. Preparation of sprays

[0122] According to the ratio in Table 3, prepare the raw materials of each spray, and measure them by mass percentage. The K value of the ratio is the mass ratio of 1,3-butanediol and PEG-10 tri(trimethylsiloxy) silyl ethyl dimethicone.

[0123] Table 3: Composition of sprays

[0124]

[0125]

[0126] The preparation method of the above sprays is as follows:

[0127] Add cyclopentasiloxane, octyl trimethylsiloxysilicate, dimethicone, butanediol, polymethylsilsesquioxane, ethylhexyl salicylate, diethylamino hydroxybenzoyl hexyl benzoate, octocrylene, lauryl PEG-10 tri(trimethylsiloxy) silyl ethyl dimethicone, and ethylhexyl triazone into a container, heat to 82°C, and stir to mix. Then add water, continue stirring at the same speed for 30 minutes. Cool to 28°C, then add the Centella asiatica extract and the Gentiana scabra extract (not used in spray 5), continue stirring at the same speed for 15 minutes to mix, sterilize, and obtain the spray.

[0128] 2. Testing of sprays

[0129] Test the above sprays for sunscreen effect, use feeling, and stability.

[0130] Sunscreen effect test: Use a UV-2000S cosmetic sunscreen index analyzer for testing. The specific steps are as follows: weigh the test sample, evenly apply the test sample to the sample plate, place it in a cool place to dry naturally for 30 minutes, then test to obtain the SPF value. The larger the value, the better the sunscreen effect.

[0131] Sensory test: 10 volunteers were selected to perform the sensory test of refreshing and non-greasy feeling for different sprays, the highest score was 10 points and the lowest score was 1 point, and then the average value of the scores of each spray was calculated.

[0132] Stability test: 50 mL of the spray was loaded in a transparent glass bottle, and the appearance of the spray was observed after being placed at 55℃ for 1 week.

[0133] The specific test results are shown in Table 4.

[0134] Table 4: SPF, sensory and stability tests of the spray

[0135] Test Sunscreen SPF value Sensory score Stability Spray 1 47.6 9.6 No separation, no sediment Spray 2 47.4 9.5 No separation, no sediment Spray 3 46.2 8.9 No separation, slight flocculation Spray 4 45.1 8.5 Appearance hazy Spray 5 46.8 9.4 No separation, no sediment

[0136] According to the test in Table 4, the SPF value of the prepared spray is 47.6, the refreshing and non-greasy score is 9.6, and no separation and precipitation are generated after being placed at 55℃ for 1 week; the spray has excellent sunscreen effect, refreshing and non-greasy feeling after application, and good temperature stability.

[0137] According to the tests of sprays 1, 3-4, even if the amount of emulsifier PEG-10 tri (trimethylsiloxy) silylethyl polydimethylsiloxane is increased or the amount of butylene glycol is increased, when the ratio of butylene glycol and PEG-10 tri (trimethylsiloxy) silylethyl polydimethylsiloxane is not within the range of 1.5-2.2:1, the product uniformity is poor, which leads to poor active ingredient distribution and use effect, thereby reducing the SPF test value, and also reducing the product stability and use effect.

[0138] Obviously, those skilled in the art can make various modifications and variations to the present application without departing from the spirit and scope of the present application. Thus, if these modifications and variations of the present application fall within the scope of the claims of the present application and their equivalents, the present application also intends to include these modifications and variations.

Claims

1. A soothing and sunscreen spray, characterized in that According to the percentage by mass, the raw materials are as follows: 1-5% cyclopentasiloxane, 0.5-3% octyl polymethicone, 0.5-2% polydimethylsiloxane, 8-12% butylene glycol, 0.5-2% polymethylsilsesquioxane, 0.4-1.5% ethylhexyl salicylate, 0.3-1.5% diethylamino hydroxybenzoyl hexyl benzoate, 0.3-1.5% octocrylene, 4-8% lauryl PEG-10 Tris(trimethylsiloxy)silylethyl polydimethicone, 0.4-1.5% ethylhexyl triazone, 0.5-2% Centella asiatica extract, 0.5-1.8% gentiana extract, and the balance water; the mass ratio of Centella asiatica extract and gentiana extract is 0.8-1:1, and the mass ratio of butylene glycol and lauryl PEG-10 tris(trimethylsiloxy)silylethyl polydimethicone is 1.5-2.2:1; The preparation method of the Centella asiatica extract comprises: crushing dried whole Centella asiatica, adding water, heating and keeping warm to obtain product 1; sterilizing product 1, adding bromelain, glucose, dipotassium hydrogen phosphate and fermentation bacteria, fermenting and sterilizing to obtain product 2; filtering product 2 to obtain a filtrate, and drying to obtain the Centella asiatica extract; the amount of bromelain added is 600-1500 U / g of product 1; the fermentation bacteria are Lactobacillus reuteri CICC 6122 and Lactobacillus plantarum CICC 20322 in a quantitative ratio of 1:0.4-0.8; The preparation method of the gentian extract comprises: crushing dried gentian roots, adding an ethanol aqueous solution with a mass of 8-40 times that of the gentian roots, keeping warm at 50-65° C. and stirring for 3-10 hours to obtain an intermediate product 1; filtering the intermediate product 1, ultrafiltration, and then concentrating under reduced pressure to 0.05-0.12 times the mass of the intermediate product 1, and drying to obtain the gentian extract; the mass concentration of the ethanol aqueous solution is 40-55%; and ultrafiltration is performed using an ultrafiltration membrane with a molecular weight cutoff of 500 Da.

2. A soothing and sunscreen spray according to claim 1, characterized in that: The amount of glucose added is 1-4% of the mass of product 1.

3. A soothing and sunscreen spray according to claim 1, characterized in that: The amount of dipotassium hydrogen phosphate added is 0.02-0.1% of the mass of product 1.

4. A soothing and sunscreen spray according to claim 1, characterized in that: The fermentation bacteria dosage was 1×10 9 -9×10 9 cfu / g product 1.

5. A method for preparing the soothing and sunscreen spray according to any one of claims 1 to 4, characterized in that: The method comprises the following steps: adding cyclopentasiloxane, caprylyl methicone, polydimethylsiloxane, butylene glycol, polymethylsilsesquioxane, ethylhexyl salicylate, diethylamino hydroxybenzoyl hexyl benzoate, octocrylene, lauryl PEG-10 tris(trimethylsiloxy)silylethyl polydimethicone and ethylhexyl triazone into a container, heating the container to 60-85°C, and stirring to mix; then adding water and stirring; cooling the container, adding Centella asiatica extract and Gentiana dahurica extract, stirring to mix, and sterilizing the container to obtain the soothing and sunscreen spray.

6. Use of the soothing and sunscreen spray according to any one of claims 1 to 4 in non-therapeutic cosmetics.

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