Tumor treatment device

Abstract

The present invention provides a tumor treatment device, comprising: an AC signal generating device, a transmitting device and a receiving device; the AC signal generating device is connected to the transmitting device, and a boosting structure is provided in the transmitting device for converting the medium-frequency low-voltage AC signal into a first voltage signal; the output end of the boosting structure is connected to a first electrode; the receiving device is arranged opposite to the transmitting device and connected to the AC signal generating device, and a transformer structure is provided in the receiving device; the output end of the transformer structure is connected to a second electrode for receiving the induced voltage signal loaded by the transformer structure and coupling with the first electrode to transmit electromagnetic wave signals of multiple frequencies. The present invention couples the second electrode with the first electrode and transmits electromagnetic wave signals of multiple frequencies, so that the target object receives the electromagnetic wave radiation at the optimal position, thereby inhibiting the mitosis of its tumor tissue and cells.

Description

Technical Field

[0001] The present invention relates to the technical field of medical equipment, and in particular to a tumor treatment device. Background Art

[0002] Using electric fields to treat tumors is one of the current research and development frontiers. Tumor electric field therapy is a therapy implemented through portable, non-invasive medical devices. Its basic principle is based on the fact that electric fields can hinder the mitosis of tumor cells. Different frequencies, directions and intensities of electric fields all show different tumor inhibition effects.

[0003] Therapeutic electric field therapy (TTFields) is a portable, non-invasive local physical therapy that generates a low-intensity, medium-frequency electric field of a specific frequency by placing a special insulated electrode patch on the patient's skin surface. The electric field can penetrate the skin and body tissues and act directly on the tumor site, thereby interfering with the mitosis of tumor cells and ultimately inhibiting tumor growth and spread.

[0004] TTFields was approved by the U.S. Food and Drug Administration (FDA) as early as 2015 for the treatment of newly diagnosed glioblastoma (GBM). It has since been approved for the treatment of recurrent glioblastoma (rGBM) and malignant pleural mesothelioma. The therapy can also be used to treat extracranial tumors such as non-small cell lung cancer, pancreatic cancer, gastric cancer, liver cancer, and ovarian cancer.

[0005] Currently, the main product of tumor therapy field devices is the Optune product developed by Novocure, a US biotechnology company. This product primarily consists of a signal generator and electrodes. During the therapy process, the signal generator generates electric field energy, which is distributed to the electrodes. The electrodes couple this energy into the human body, applying an alternating electric field to the tissue area where the tumor is located.

[0006] However, TTFields has the following three major disadvantages when used:

[0007] First, the electrodes must be in close contact with the skin during treatment. For glioma treatment, the patient's hair is shaved, and electrodes are applied to the shaved scalp. The electrodes transmit a low-intensity, medium-frequency AC electric field, selectively disrupting cell division. Recent clinical data indicates that TTF treatment can cause adverse reactions, including moderate skin irritation and contact dermatitis.

[0008] Secondly, due to contact treatment, space and technical limitations, TTFields cannot apply a larger electric field and cannot produce greater tumor cell killing ability. It can only inhibit the tumor division rate and often needs to be used in combination with other treatment options (drugs, chemotherapy).

[0009] Finally, TTFields needs to be worn for more than 18 hours a day, which seriously affects the patient's quality of life and the patient experience is not good. Summary of the Invention

[0010] In view of this, the present invention proposes a tumor treatment device, which aims to solve the problems of poor user experience and weak electric field generated by the existing tumor treatment devices.

[0011] The present invention proposes a tumor treatment device, comprising: an AC signal generating device, a transmitting device and a receiving device; wherein,

[0012] The AC signal generating device is connected to the transmitting device, and is used to generate a medium-frequency low-voltage AC signal and transmit the medium-frequency low-voltage AC signal to the transmitting device;

[0013] The transmitting device is provided with a boost structure for converting the medium-frequency low-voltage AC signal into a first voltage signal; the voltage value of the first voltage signal is greater than the voltage value of the medium-frequency low-voltage AC signal, and the output end of the boost structure is connected to a first electrode for receiving the first voltage signal applied by the boost structure;

[0014] The receiving device is arranged opposite to the transmitting device and connected to the AC signal generating device. The receiving device is provided with a voltage transformation structure for matching with the boost structure to generate an induced voltage signal. The voltage value of the induced voltage signal is less than the voltage value of the first voltage signal, thereby generating a high-field-strength electric field between the transmitting device and the receiving device.

[0015] The output end of the transformer structure is connected to a second electrode, the second electrode is used to receive the induced voltage signal loaded by the transformer structure and couple with the first electrode to emit electromagnetic wave signals of multiple frequencies;

[0016] The first electrode and the second electrode both have a frame structure, the ends of the frame structure are provided with a notch, and both ends of the notch are provided with arc-shaped contacts to prevent arc discharge;

[0017] A seat mechanism is provided in the area between the receiving device and the transmitting device, so as to enable the target object to receive high-field-strength and multi-frequency electromagnetic wave radiation at an optimal position.

[0018] Furthermore, in the above-mentioned tumor treatment device, the frame structure is a ring frame, and there are multiple ring frames, and the multiple ring frames are concentrically arranged to form a concentric circle structure, and the contact connection lines at both ends of each notch are arranged at an angle.

[0019] Furthermore, in the above-mentioned tumor treatment device, a first fastener is provided on the frame structure of the first electrode for connecting to the transmitting device; and / or a second fastener is provided on the frame structure of the second electrode for connecting to the receiving device.

[0020] Furthermore, in the above-mentioned tumor treatment device, the first fastener and the second fastener each comprise: at least two intersecting connecting plates; wherein,

[0021] Each of the connecting plates is provided with a plurality of slots or connecting holes corresponding to each other, for fixing to the corresponding frame structure respectively.

[0022] Furthermore, in the above-mentioned tumor treatment device, the first electrode is electrically connected to the output end of the boost structure through a first high-voltage wire; and / or the second electrode is electrically connected to the output end of the transformer structure through a second high-voltage wire.

[0023] Furthermore, in the above-mentioned tumor treatment device, the boost structure and the voltage transformation structure are both Tesla coils, and the frequencies of the two Tesla coils are the same.

[0024] Furthermore, in the above-mentioned tumor treatment device, the transmitting device and the receiving device are both provided with an insulating shell, and a roller is provided at the bottom of the insulating shell.

[0025] Furthermore, in the above-mentioned tumor treatment device, indicator plates are respectively provided on the insulating housing in areas corresponding to the first electrode and the second electrode, so as to align the user's part to be treated with the areas where the first electrode and the second electrode are located.

[0026] Furthermore, in the above-mentioned tumor treatment device, the indicator plate is circular or polygonal.

[0027] Furthermore, in the above-mentioned tumor treatment device, a first support frame is arranged in the insulating shell of the transmitting device, and the boosting structure is arranged on the first support frame; and / or a second support frame is arranged in the insulating shell of the receiving device, and the transformer structure is arranged on the second support frame.

[0028] The tumor treatment device provided by the present invention uses a boosting structure in a transmitting device to boost a medium-frequency low-voltage alternating current signal to a first voltage signal with a higher voltage value, and loads it onto a first electrode. At the same time, a transformer structure and a boosting structure in a receiving device cooperate to generate an induced voltage with a voltage value lower than the first voltage signal, and load it onto a second electrode. The second electrode couples with the first electrode and emits electromagnetic wave signals of multiple frequencies, so that the target object receives the electromagnetic wave radiation at the optimal position, thereby inhibiting the mitosis of its tumor tissue and cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Various other advantages and benefits will become apparent to those skilled in the art upon reading the detailed description of the preferred embodiment below. The accompanying drawings are for illustration purposes only and are not to be considered as limiting the present invention. The same reference symbols are used throughout the drawings to represent the same components. In the drawings:

[0030] Figure 1 A schematic diagram of the structure of a tumor treatment device provided by an embodiment of the present invention;

[0031] Figure 2 An axial view of the interior of a transmitting device or a receiving device according to an embodiment of the present invention;

[0032] Figure 3 is a schematic structural diagram of the first electrode or the second electrode in an embodiment of the present invention;

[0033] Figure 4 is an equivalent electronic circuit diagram of the resonance process of the first electrode and the second electrode in an embodiment of the present invention;

[0034] Figure 5 1 is a waveform diagram of a high-field-strength multi-frequency electromagnetic wave in an embodiment of the present invention;

[0035] Figure 6 This is a graph comparing the growth curve of U251 glioma cells treated with the non-invasive tumor treatment device provided by an embodiment of the present invention and the growth curve of U251 glioma cells in a normal incubator;

[0036] Figure 7 This is a graph comparing the apoptosis curve of U251 glioma cells treated with the non-invasive tumor treatment device provided in an embodiment of the present invention and the apoptosis curve of U251 glioma cells in a normal incubator;

[0037] Figure 8 This is a graph comparing the apoptosis curve of MCF7 breast cancer cells treated with the non-invasive tumor treatment device provided in an embodiment of the present invention and the apoptosis curve of MCF7 breast cancer cells in a normal incubator;

[0038] Figure 9 This is a comparison chart of the apoptosis curve of HELA cells treated with the non-invasive tumor treatment device provided in an embodiment of the present invention and the apoptosis curve of HELA cells in a normal incubator. DETAILED DESCRIPTION

[0039] The exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. Although exemplary embodiments of the present disclosure are shown in the accompanying drawings, it should be understood that the present disclosure can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present disclosure and to fully convey the scope of the present disclosure to those skilled in the art. It should be noted that, unless there is a conflict, the embodiments of the present disclosure and the features described in the embodiments can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0040] See Figures 1 to 3 The tumor treatment device of the embodiment of the present invention includes: an AC signal generating device 1, a transmitting device 2 and a receiving device 3; wherein the AC signal generating device 1 is connected to the transmitting device 2 to generate a medium-frequency low-voltage AC signal and transmit the medium-frequency low-voltage AC signal to the transmitting device 2, and a boosting structure 21 is provided in the transmitting device 2 to convert the medium-frequency low-voltage AC signal into a first voltage signal; the voltage value of the first voltage signal is greater than the voltage value of the medium-frequency low-voltage AC signal, and the output end of the boosting structure 21 is connected to a first electrode 41 for receiving the first voltage signal loaded by the boosting structure 21; the receiving device 3 is arranged opposite to the transmitting device 2 and connected to the AC signal generating device 1, and a transformer structure 31 is provided in the receiving device 3 to The boost structure 21 matches to generate an induced voltage signal, the voltage value of the induced voltage signal is less than the voltage value of the first voltage signal; thereby generating a high-field-strength electric field between the transmitting device 2 and the receiving device 3; the output end of the transformer structure 31 is connected to a second electrode 42, the second electrode 42 is used to receive the induced voltage signal loaded by the transformer structure 31 and couple with the first electrode 41 to transmit electromagnetic wave signals of multiple frequencies; the first electrode 41 and the second electrode 42 both have a frame structure 40, the end of the frame structure 40 is provided with a notch a, and both ends of the notch a are provided with arc contacts b to prevent arc discharge; the area between the receiving device 3 and the transmitting device 2 is provided with a seat mechanism to enable the target object to receive high-field-strength and multi-frequency electromagnetic wave radiation in the optimal position.

[0041] Specifically, the transmitting device 2 and the receiving device 3 may be a cabinet structure, such as a square cabinet.

[0042] The first electrode 41 is electrically connected to the output end of the boost structure 21 via a first high-voltage wire (not shown in the figure); and / or the second electrode 42 is electrically connected to the output end of the transformer structure 31 via a second high-voltage wire (not shown in the figure).

[0043] The input of the boost structure 21 can be connected to the AC signal generator 1 via a first control line 7, and the input of the transformer structure 31 can be connected to the AC signal generator 1 via a second control line 8. The first control line 7 is threaded through the columns of the first support frame 5 inside the insulating housing, and the second control line 8 is threaded through the columns of the second support frame 6 inside the insulating housing. The AC signal generator 1 can be connected to an external 220V power supply.

[0044] The frame structure 40 of the first electrode 41 and the second electrode 42 can be a ring frame or a polygonal frame, such as a square frame, a trapezoidal frame, a rectangular frame, etc. Preferably, the frame structure is a ring frame. There can be one or more frame structures 40.

[0045] In this embodiment, there is capacitance between the frame structure of the first electrode 41 and the frame structure of the second electrode 42 and the ground, and there is damping in the resonance process of the circuit formed by the Tesla coil forming the boost structure and the Tesla coil forming the transformer structure. Figure 4 , which can be described by a linear second-order differential equation:

[0046]

[0047] In the formula: R is the resistance, Uc represents the effective value of the voltage on the capacitor, L is the inductance value, C is the capacitance value, I is the current, U is the voltage value, and t is the time.

[0048] Final formation Figure 5 From the damped oscillation attenuation waveform in , we can see that the capacitance, voltage, and current all oscillate with equal amplitude, with a 90° phase difference between the voltage and current. The capacitance voltage oscillates sinusoidally between U0 and -U0, and the current oscillates sinusoidally around zero.

[0049] Electromagnetic wave signals based on this waveform propagate in non-ideal conditions in space. The amplitude-frequency characteristics of capacitors and inductors are primarily reflected in their responses to signals of varying frequencies. In AC circuits, capacitors and inductors exhibit different frequency responses: capacitors pass high frequencies and block low frequencies. In AC circuits, the impedance of a capacitor decreases with increasing frequency, resulting in less resistance to high-frequency signals and greater resistance to low-frequency signals. When the amplitude changes, the frequency characteristics of capacitors and inductors at different amplitudes do not directly change due to changes in signal amplitude. Ideally, the impedance of capacitors and inductors is a function of frequency, not signal amplitude. That is, for a given frequency, the impedance of capacitors and inductors remains constant. However, in practical applications, when the signal amplitude changes, non-ideal effects may be observed, which can indirectly affect the apparent capacitance and inductance, thereby affecting the frequency response. Therefore, under non-ideal conditions, changes in the amplitude of the electromagnetic wave will cause changes in the capacitance and inductance characteristics, thereby causing changes in the frequency, thereby forming a multi-frequency electromagnetic wave signal. In this embodiment, the frequency of the electromagnetic wave signal is between 10kHz-1000Khz.

[0050] The target subject is placed in the high-strength electric field environment formed between the transmitter and receiver in the embodiments of the present invention, receiving medium-frequency electromagnetic wave energy and signals. The medium-frequency electromagnetic waves polarize biological tissue through electromagnetic attraction, altering the distances between atoms and atomic groups within molecules, thereby changing the tissue's electromagnetic properties, such as dielectric constant and magnetic permeability.

[0051] At the same time, proteins, lipids, and amino acids absorb energy from radiofrequency electromagnetic waves through frequency resonance. This causes changes in their structure, conformation, and configuration. The absorbed energy also causes their dipole moment, dielectric constant, and magnetic permeability to vary significantly with frequency. These changes can affect mitochondria, providing energy to cells and eliminating common inflammation, thereby activating the patient's own anti-tumor immune response.

[0052] More importantly, under the influence of external electromagnetic forces, the target tumor cells can induce a dipole moment, creating a potential difference around the cellular tissue, thus forming an internal electric field within the biological tissue. This internal electric field acts on the microtubules of proliferating cancer cells during the late stages of cell division, thereby interfering with mitosis, inhibiting tumor growth and causing the cell death of the affected cells. The electric field can selectively interfere with cancer cells in the rapid proliferation and division phase, which produces highly charged species, causing them to undergo programmed cell death through cellular suicide, while having no significant effect on normal human cells in the quiescent and dividing phases.

[0053] The arc contacts b at both ends of the notch a at the end of the frame structure 40 can be spherical structures to match the size of the frame structure 40, so that the surface of the frame structure 40 maintains a certain curvature, thereby reducing the risk of high-voltage tip discharge.

[0054] The chair mechanism can be a lifting chair so that the target object can adjust its position so that the area to be treated is aligned with the transmitting area and the receiving area, thereby preventing other parts from being irradiated by electromagnetic waves.

[0055] In this embodiment, the transmitting device 2 and the receiving device 3 are both provided with an insulating shell, and a roller, such as a universal wheel, is provided at the bottom of the insulating shell.

[0056] Furthermore, indicator plates 45 are provided on the insulating housing in areas corresponding to the first electrode 41 and the second electrode 42, respectively, for aligning the user's treated area with the areas where the first electrode 41 and the second electrode 42 are located. Preferably, the indicator plates 45 are circular or polygonal.

[0057] A first support frame 5 is provided in the insulating shell of the transmitting device 2, and the boosting structure 21 is provided on the first support frame 5; and / or a second support frame 6 is provided in the insulating shell of the receiving device 3, and the voltage transformation structure 31 is provided on the second support frame 6.

[0058] Specifically, the first support frame 5 and the second support frame 6 each comprise a base plate, multiple support columns, and a top plate. The support columns are connected between the base plate and the top plate. The boost structure 21 is disposed on the top plate of the first support frame 5, with the first electrode 41 connected to the output end of the boost structure 21. The transformer structure 31 is disposed on the top plate of the second support frame 6, with the second electrode 42 connected to the output end of the transformer structure 31. The top plate can be convex-shaped and is provided with a bracket to support the boost structure 21 or transformer structure 31. The top of the bracket can be an arc-shaped groove to conform to the outer wall of the boost structure 21.

[0059] In specific implementation, the boost structure 21 is arranged in the horizontal direction, and the first electrode 41 is arranged in the vertical direction at the output end of the boost structure 21; the transformer structure 31 is arranged opposite to the boost structure 21 in the horizontal direction, and the second electrode 42 is arranged in the vertical direction at the output end of the transformer structure 31.

[0060] In this embodiment, the boost structure 21 and the transformer structure 31 are both Tesla coils, and the frequencies of the two Tesla coils are the same.

[0061] When a Tesla coil is operating, it creates an alternating magnetic field in the surrounding space. If another Tesla coil is located within the effective range of this alternating magnetic field and its resonant frequency matches or is close to that of the transmitting coil, the conductor in the receiving coil will feel the changing magnetic field, inducing current and voltage within it.

[0062] In this embodiment, the boost structure 21 can boost the medium-frequency low-voltage AC signal to above 100 kV, and the second electrode 42 connected to the transformer structure 31 can generate an induced voltage of not less than 50 kV. Due to the large potential difference, a high-field-strength electric field is formed between the transmitting device 2 and the receiving device 3.

[0063] In this embodiment, the AC signal generating device 1 can be any device known in the art. For example, the AC signal generating device 1 includes a plug for receiving 220V AC power. The AC signal generating device 1 in this embodiment of the present invention can include: a single-chip microcomputer control circuit and a touch screen; the single-chip microcomputer control circuit is connected to the touch screen to receive command signals from the touch screen and adjust the current and voltage in the circuit to generate a medium-frequency low-voltage AC signal.

[0064] In actual use, the touch screen controls the parameters of the core electric field signal output. Key core parameters include electric field waveform, primary frequency, pulse width, pulse number, and duration. Based on tumor type and imaging data, the touch screen allows for fine-tuning of these parameters, enabling AC signal generator 1 to output intermediate-frequency electric field signals of varying frequencies for transmission to transmitter 2.

[0065] It can be clearly concluded from the above that the tumor treatment device provided in this embodiment boosts the medium-frequency low-voltage AC signal to a first voltage signal with a higher voltage value through the boost structure 21 in the transmitting device 2, and loads it onto the first electrode 41. At the same time, the transformer structure 31 in the receiving device 3 cooperates with the boost structure 21 to generate an induced voltage with a voltage value lower than the first voltage signal, and loads it onto the second electrode 42. The second electrode 42 couples with the first electrode 41 and emits electromagnetic wave signals of multiple frequencies, so that the target object receives the electromagnetic wave radiation at the optimal position, thereby inhibiting the mitosis of its tumor tissue and cells. The first electrode 41 and the second electrode 42 do not need to contact the target object, which optimizes the user experience of the target object while being able to generate a higher field strength electric field and multi-frequency electromagnetic waves, greatly enhancing the inhibition rate of tumor cells.

[0066] Continue reading Figure 3 In the above embodiment, the frame structure 40 is a ring frame, and there are multiple ring frames. The multiple ring frames are concentrically arranged to form a concentric circle structure, and the contact connection lines at both ends of each notch a are arranged at an angle c.

[0067] Specifically, in the concentric circle structure, the annular frames may be arranged at equal intervals, or may be arranged in a manner such that the intervals decrease from the outer circle to the inner circle.

[0068] In a specific implementation of this embodiment, in the concentric circle structure, the diameters of the metal tubes constituting the annular frames are all equal.

[0069] Preferably, the diameter of the metal tube constituting the annular frame decreases from the outermost circle to the innermost circle of the concentric circle structure, which is beneficial to reducing the risk of air discharge effect generated by the first electrode 41 and the second electrode 42.

[0070] In a specific implementation of the embodiment of the present invention, the notches a of the annular frames are arranged in an array along the same direction.

[0071] Preferably, the contact lines at both ends of each notch a are arranged at an angle of 20-40°, preferably 34°, which can widen the radiation range of the electromagnetic wave and improve the overall gain of the array structure formed by each notch a.

[0072] In another specific implementation of the embodiment of the present invention, the notches a of two adjacent annular frames are arranged in opposite directions and are positioned opposite to each other.

[0073] Furthermore, a first fastener 43 is provided on the frame structure of the first electrode 41 for connecting with the transmitting device 2; and / or a second fastener 44 is provided on the frame structure of the second electrode 42 for connecting with the receiving device 3.

[0074] In a specific implementation, the first fastener 43 and the second fastener 44 are both made of insulating materials, such as polyoxymethylene. The first fastener 43 and the second fastener 44 have the same structure.

[0075] More specifically, the first fastener 43 and the second fastener 44 each include: at least two intersecting connecting plates 400; wherein each connecting plate 400 is provided with a plurality of corresponding slots 4001 or connecting holes for fixing to the corresponding frame structure 40 respectively.

[0076] Specifically, the connecting plates 400 may be strip-shaped plates with curved ends, and any two adjacent connecting plates 400 are arranged at a preset angle. For example, when there are two connecting plates 400, the two connecting plates 400 are arranged vertically.

[0077] The diameter of each slot 4001 on the connecting plate 400 matches the thickness of each frame structure 40 to ensure that each frame structure 40 is firmly connected to the slot 4001. Each slot 4001 is provided in a one-to-one correspondence with each frame structure 40, that is, the number of slots is consistent with the number of frame structures 40.

[0078] Of course, a plurality of connection holes (not shown) may also be provided on the connection plate 400. The connection holes on each connection plate 400 are provided in pairs, for sequentially passing connection straps (not shown) therethrough, thereby securing each frame structure 40 to the connection plate 400. For example, a pair of connection holes may be symmetrically provided on both sides of each frame structure 40, so that the connection straps can be tied to the frame structure 40. The connection straps may be made of an insulating material, such as polyoxymethylene.

[0079] To verify the inhibitory effect of the tumor treatment device provided in the embodiments of the present invention on tumor cells, the present invention used the device to conduct a cell growth curve test on U251 brain glioma cells and an apoptosis test on three tumor cell types: U251 brain glioma cells, MCF7 cervical cancer cells, and Hela uterine cancer cells. The test methods are as follows:

[0080] Experimental method 1: cell viability assay

[0081] Experimental Preparation: Cells were plated the day before the experiment and monitored using an Olympus CKX53 inverted fluorescence microscope. U251 glioma cells at 70%-80% confluence were selected and rinsed with Gibco Ph7.4 PBS. Cells were then digested with Gibco 0.25% trypsin for 90 seconds at room temperature. Cells were harvested and diluted to a concentration of 20,000 cells / ml using Procell DMED complete medium. 2,000 cells were plated per well in COSTAR 96-well clear cell culture plates at 100 μl per well. One plate was used for the control group, and the number of plates for the experimental group was determined according to the experimental protocol. Three wells were plated in parallel, and three wells of blank culture medium were used as a monitoring point. The number of monitoring points per plate was determined according to the experimental requirements. Cells were cultured overnight in a 37°C, 5% BPN-150CH (UV) CO2 incubator.

[0082] On the day of the experiment

[0083] The first test was performed 90 minutes before the start of the experiment. 10 μl of MCE CellCounting Kit-8 was added to the 6 wells of the monitoring point, and the cells were incubated in a 37°C, 5% BPN-150CH (UV) carbon dioxide incubator for 90 minutes. The absorbance reading was performed using a MIULAB DEL-100 microplate reader at a 450 nm filter. The reading was obtained by subtracting the blank control blank mean from the cell null mean. Subsequently, each monitoring point was processed in the same manner.

[0084] During the experiment, the 96-well plate was placed in a homemade insulated constant temperature box at 37°C and 5% carbon dioxide and placed between the machines for treatment. The test was performed at different time points according to the experimental requirements. The time distribution of each monitoring point was recorded as the horizontal axis, and the absorbance was recorded as the vertical axis. The growth curve was obtained using a broken line graph.

[0085] By comparing the growth rates of the growth curves of the experimental group and the control group, the doubling time at different time points was calculated and compared, and a one-way analysis of variance was performed at each point to obtain the P value to judge the validity of the results.

[0086] Experimental method 2: cell apoptosis detection

[0087] Experimental Preparation: Cells were plated the day before the experiment and monitored using an Olympus CKX53 inverted fluorescence microscope. U251 glioma cells at 70%-80% confluence were selected and rinsed with GIBCO pH 7.4 PBS. Cells were then digested with GIBCO 0.25% trypsin for 90 seconds at room temperature. Cells were harvested and diluted to a concentration of 40,000 cells / ml using DMED complete medium. 2,000 cells were plated per well in COSTAR 96-well white cell culture plates, with 50 μl of culture medium per well. One control plate was used, and the number of experimental plates plated was determined according to the experimental protocol. Three replicate wells were used for the blank culture medium control. The plates were placed in a 37°C, 5% CO2 incubator (BPN-150CH (UV)). At 7-8 PM, 50 μl of the prepared RealTime-Glo™ Annexin V Apoptosis Assay 2X working solution was added, mixed thoroughly, and returned to the incubator.

[0088] On the day of the experiment: Luminescence readings were taken before the experiment began using a PROMEGA GLOMAX DISCOVER multi-function microplate reader. The reading for each well was 0.3 seconds, and the blank control null mean was subtracted from the cell null mean. Readings were taken every 2 hours during the experiment, with the time distribution of each monitoring point plotted as the horizontal axis and the luminescence reading as the vertical axis. A line graph was used to generate a cell apoptosis kinetic curve.

[0089] The experimental results were judged by comparing the kinetics and numerical values ​​of the apoptosis curves of the experimental group and the control group.

[0090] In addition, the test method for MCF7 breast cancer cells and HELA cervical cancer cells is the same as the above-mentioned cell apoptosis detection test.

[0091] The results of the above cell viability and apoptosis detection experiments are as follows Figure 6-9 As shown, through Figure 6It can be seen that the U251 glioma cells after electric field radiation grow slower than the cells in the incubator. Figure 6 Middle: L1 is the growth curve of U251 glioma cells after being treated with the non-invasive tumor treatment device provided by the embodiment of the present invention, and L2 is the growth curve of U251 glioma cells in a normal incubator.

[0092] pass Figure 7 It can be seen that the U251 glioma cells subjected to electric field radiation have a more obvious apoptosis trend than the cells in the incubator. Figure 7 Middle: L3 is the apoptosis curve of U251 glioma cells after being treated with the non-invasive tumor treatment device provided by an embodiment of the present invention, and L4 is the apoptosis curve of U251 glioma cells in a normal incubator.

[0093] Likewise, through Figure 8-9 It can be clearly seen that the electric field generated by the device provided by the present invention can induce apoptosis of MCF7 breast cancer cells and HELA cells. Figure 8 Middle: L5 is the apoptosis curve of MCF7 breast cancer cells after being treated with the non-invasive tumor treatment device provided by an embodiment of the present invention; L6 is the apoptosis curve of MCF7 breast cancer cells in a normal incubator; Figure 9 Middle: L7 is the apoptosis curve of HELA cervical cancer cells after being treated with the non-invasive tumor treatment device provided by an embodiment of the present invention, and L8 is the apoptosis curve of HELA cervical cancer cells in a normal incubator.

[0094] The above-mentioned cell viability and apoptosis detection experiments demonstrate that the electric field generated by the device provided by the present invention has an inhibitory effect on tumor cells and can also induce tumor cell apoptosis. Compared with the existing technology, the present invention has the following advantages:

[0095] (1) It is completely a physical therapy, non-invasive and has no side effects; (2) It is a non-contact treatment that will not cause discomfort to the patient and is very convenient to operate.

[0096] In summary, the tumor treatment device provided in this embodiment uses a boosting structure in the transmitting device to boost the medium-frequency low-voltage AC signal to a first voltage signal with a higher voltage value, and loads it onto the first electrode. At the same time, the transformer structure and the boosting structure in the receiving device cooperate to generate an induced voltage with a voltage value lower than the first voltage signal, and load it onto the second electrode. The second electrode couples with the first electrode and emits electromagnetic wave signals of multiple frequencies, so that the target object receives the electromagnetic wave radiation at the optimal position, thereby inhibiting the mitosis of its tumor tissue and cells.

[0097] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if such changes and modifications fall within the scope of the claims and their equivalents, the present invention is intended to include such changes and modifications.

Claims

1. A tumor treatment device, characterized in that: include: AC signal generating device, transmitting device and receiving device; in, The AC signal generating device is connected to the transmitting device, and is used to generate a medium-frequency low-voltage AC signal and transmit the medium-frequency low-voltage AC signal to the transmitting device; The transmitting device is provided with a boost structure for converting the medium-frequency low-voltage AC power signal into a first voltage signal; the voltage value of the first voltage signal is greater than the voltage value of the medium-frequency low-voltage AC power signal and is greater than 100 kV; the output end of the boost structure is connected to a first electrode for receiving the first voltage signal applied by the boost structure; The receiving device is arranged opposite to the transmitting device and connected to the AC signal generating device. The receiving device is provided with a transformer structure for matching with the boost structure to generate an induced voltage signal. The voltage value of the induced voltage signal is less than the voltage value of the first voltage signal, thereby generating a high-field-strength electric field between the transmitting device and the receiving device. The voltage value of the induced voltage signal is not less than 50 kV. The output end of the transformer structure is connected to a second electrode, which is used to receive the induced voltage signal loaded by the transformer structure and couple with the first electrode to emit electromagnetic wave signals of multiple frequencies with damped oscillation attenuation characteristics, wherein the frequency range of the electromagnetic wave signal is 10kHz-1000kHz; The first electrode and the second electrode each have a frame structure, with notches at the ends of the frame structure, and arc-shaped contacts at both ends of the notches to prevent arc discharge; the frame structure is an annular frame, and the annular frames are multiple, and the multiple annular frames are concentrically arranged to form a concentric circle structure, and the contact lines at both ends of each notch are arranged at an angle of 20-40 degrees; the notches of each annular frame are arranged in an array along the same direction; the boost structure and the transformer structure are both Tesla coils, and the frequencies of the two Tesla coils are the same; A seat mechanism is provided in the area between the receiving device and the transmitting device, so that the target object can receive high-field-strength and multi-frequency electromagnetic wave radiation at an optimal position.

2. The tumor treatment device according to claim 1, characterized in that: A first fastener is provided on the frame structure of the first electrode for connecting with the transmitting device; and / or a second fastener is provided on the frame structure of the second electrode for connecting with the receiving device.

3. The tumor treatment device according to claim 2, characterized in that: The first fastener and the second fastener each include: at least two intersecting connecting plates; wherein, Each of the connecting plates is provided with a plurality of slots or connecting holes corresponding to each other, for fixing to the corresponding frame structure respectively.

4. The tumor treatment device according to claim 1, characterized in that: The first electrode is electrically connected to the output end of the boost structure via a first high-voltage wire; and / or the second electrode is electrically connected to the output end of the transformer structure via a second high-voltage wire.

5. The tumor treatment device according to claim 1, characterized in that: The transmitting device and the receiving device are both provided with an insulating shell, and a roller is provided at the bottom of the insulating shell.

6. The tumor treatment device according to claim 5, characterized in that: Indicator plates are respectively provided on the insulating shell in areas corresponding to the first electrode and the second electrode, so as to align the user's part to be treated with the areas where the first electrode and the second electrode are located.

7. The tumor treatment device according to claim 6, characterized in that: The indicator plate is circular or polygonal.

8. The tumor treatment device according to claim 5, characterized in that: A first support frame is provided in the insulating shell of the transmitting device, and the boosting structure is provided on the first support frame; and / or a second support frame is provided in the insulating shell of the receiving device, and the transformer structure is provided on the second support frame.

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