Culture dish device for mesenchymal stem cell factor collection

Abstract

The present invention belongs to the technical field of mesenchymal stem cell culture, and discloses a culture dish device for collecting mesenchymal stem cell factors, including a culture component, a connecting cavity is opened at one end of the culture component, a connecting component is arranged in the connecting cavity, a protective component is connected to the end of the connecting component away from the culture component, and at least one vibration spring is connected between the connecting cavity and the protective component; through the mutual cooperation between the provided protective component and the connecting component, the position of the vessel can be fixed, thereby facilitating the use of the vessel by the user, reducing the discomfort caused by operating the vessel, and increasing the rotation angle of the vessel, so as to enhance the performance of the vessel for the user, and at the same time cooperating with the vibration spring to assist the user to complete the uniform mixing of the solution in the vessel, fully contact with the cells inside the vessel, and improve the culture efficiency of the culture component.

Description

Technical Field

[0001] The invention belongs to the technical field of mesenchymal stem cell culture, in particular to a culture dish device for collecting mesenchymal stem cell factors. Background Art

[0002] A culture dish is a laboratory vessel used for culturing microorganisms or cells. It consists of a flat, disc-shaped base and a lid, and is generally made of glass or plastic. Culture dish materials are generally divided into two categories: plastic and glass. Glass can be used for plant materials, microbial culture, and adherent culture of animal cells. Mesenchymal stem cells are a type of pluripotent stem cell that possess all the common properties of stem cells, namely self-renewal and multidirectional differentiation capabilities. They are also the most widely used in clinical applications. Combined use with hematopoietic stem cells can improve the success rate of transplantation and accelerate hematopoietic reconstitution. The cultured cells possess the basic characteristics of mesenchymal stem cells, providing a theoretical basis for the establishment of mesenchymal stem cell banks and clinical applications.

[0003] The patent with application number CN201710351962.5 discloses a device for culturing human gingival mesenchymal stem cells, including a first traceless solid film, a roller, a wheel frame, a screw barrel, a screw, a nut, a slide rail, a first plate holder, a pillar, a slider, a limit block, a second plate holder, a suction cup, a cover, a second traceless solid film, a through hole, a push plate, a spring, a push rod, a notch and a 96-well enzyme labeling plate. A wheel frame is provided on the left side of the first plate holder, a roller is installed on the wheel frame, and the first traceless solid film is wrapped around the roller. The upper surface and right side of the first plate holder are smooth structures, and the first traceless solid film can be adhered to the upper surface and the right side of the first plate holder; the advantages of the above technical features are that the upper and lower layers of 96-well enzyme labeling plates are arranged in an overlapping manner.

[0004] At the same time, during the collection and cultivation process, the user needs to hold the culture dish to a certain position for operation according to the operation requirements. At this time, the user needs to hold the operating component in one hand and the culture dish in the other hand to operate. It is very easy to collide, causing the culture dish to fall or the operating component to be contaminated, affecting the cultivation effect of mesenchymal stem cells, and increasing the difficulty of operation for the user. Summary of the Invention

[0005] The purpose of the present invention is to solve the above problems. The present invention provides a culture dish device for collecting mesenchymal stem cell factors, which has the advantages of simple operation and high stability.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a culture dish device for collecting mesenchymal stem cell factors, comprising a culture component, wherein one end of the culture component is provided with a connecting cavity, the connecting cavity is provided with a connecting component, the end of the connecting component remote from the culture component is connected to a protective component, and at least one vibrating spring is connected between the connecting cavity and the protective component;

[0007] The connecting assembly includes a fixed sleeve, one end of which is fixedly connected to the connecting cavity, and an end of the fixed sleeve away from the culture assembly is slidably provided with a movable rod;

[0008] The protection component includes a suction cup, one side of the suction cup is connected to a connecting sleeve, an end of the connecting sleeve away from the suction cup is connected to a fixing part, and the fixing part is movably connected to the connecting component.

[0009] Preferably, the culture assembly comprises a vessel, the connecting cavity is located at one end of the vessel away from the opening, and a reinforcement ring is connected to the end of the vessel away from the connecting cavity.

[0010] Preferably, the suction cup, the connecting sleeve and the fixing part are all made of silicone material, and the fixing part is an annular body, wherein the diameter of the inner ring of the fixing part close to the culture component is larger than the diameter of the fixing part away from the culture component.

[0011] Preferably, the connecting assembly further comprises a movable plate, which is movably connected to the inner ring of the fixing portion, and the movable plate abuts against the inner ring of the fixing portion, and the movable plate will not be separated from the fixing portion.

[0012] Preferably, the connecting assembly further comprises a ball joint, a fixed end of the ball joint is connected to one end of the movable rod, and a movable end of the ball joint is connected to the movable plate.

[0013] Preferably, a docking sleeve is connected to the end of the suction cup away from the connecting sleeve, and a docking groove is opened on the inner side of the connecting sleeve. The docking groove is close to the connection position between the connecting sleeve and the suction cup, and the docking sleeve is adapted to the docking groove.

[0014] Preferably, a fold is provided at the connection position between the connecting sleeve and the suction cup.

[0015] Preferably, a movable cavity is provided in the fixing sleeve, an elastic reset device is connected to the movable cavity, and the elastic reset device is in contact with the movable rod.

[0016] Preferably, one end of the vibration spring is connected to the cavity wall of the connecting cavity, and the other end of the vibration spring is connected to the connecting sleeve.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] 1. Through the cooperation between the protective components and the connecting components, the position of the vessel can be fixed, thereby facilitating the use of the vessel by the user and reducing the discomfort caused by operating the vessel. The rotation angle of the vessel can be increased, so that the user can enhance the performance of the vessel. At the same time, the cooperation with the vibration spring can assist the user to evenly mix the solution in the vessel and fully contact the cells inside the vessel, thereby improving the culture efficiency of the culture component.

[0019] 2. Through the cooperation between the fixed sleeve, movable rod and movable plate, the height of the vessel can be fixed while the adsorption between the suction cup and the plane can be controlled, thereby improving the convenience of using the culture dish equipment and reducing the risk of the vessel falling off when the user operates the cells in the vessel.

[0020] 3. Through the protection component, when it is necessary to operate the cells inside the vessel, the position of the vessel is fixed by the suction cup and the connecting sleeve to maintain the position of the vessel. When the vessel enters the moving or incubation state, the vessel is wrapped by the suction cup and the connecting sleeve to reduce direct damage to the vessel body when the vessel collides or falls, thereby improving the safety of the vessel. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the three-dimensional structure of the overall device of the present invention;

[0022] Figure 2 A structural diagram of another angle of the present invention;

[0023] Figure 3 Schematic diagram of the connection structure between the protection component and the connection component of the present invention;

[0024] Figure 4 Schematic diagram of the cross-sectional structure of the overall device of the present invention;

[0025] Figure 5 This is a schematic diagram of the use structure of the protection component and the cultivation component of the present invention;

[0026] Figure 6 for Figure 5 Schematic diagram of the cross-section of the overall structure;

[0027] Figure 7 This is a schematic diagram of the connection structure between the fixing portion and the connecting sleeve of the present invention;

[0028] Figure 8 This is a schematic diagram of the connection structure of the docking groove of the present invention;

[0029] Figure 9 A schematic diagram of the position of the docking groove of the present invention;

[0030] Figure 10 It is a schematic diagram of the splicing structure of the overall structure of the present invention.

[0031] Description of the drawings: 1. Culture component; 101. Vessel; 102. Reinforcement ring; 103. Connecting cavity; 2. Connecting component; 201. Fixed sleeve; 202. Movable rod; 203. Ball joint; 204. Movable plate; 3. Protective component; 301. Suction cup; 302. Connecting sleeve; 3021. Docking groove; 303. Docking sleeve; 304. Fixed part; 4. Vibration spring. DETAILED DESCRIPTION

[0032] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0033] like Figure 1-Figure 3 As shown, a culture dish device for collecting mesenchymal stem cell factors includes a culture component 1, wherein the culture component 1 can carry and cultivate mesenchymal stem cells, and during the cultivation process, the cultivation status of the mesenchymal stem cells can be detected and judged by observing the cell state in the culture component 1. A connecting cavity 103 is provided at one end of the culture component 1. The setting of the connecting cavity 103 reduces the connection height of the connecting component 2 during the connection process, thereby facilitating the use of the culture dish device. A connecting component 2 is provided in the connecting cavity 103, and a protective component 3 is connected to the end of the connecting component 2 away from the culture component 1. The culture component 1 and the protective component 3 are connected in series through the connecting component 2, thereby ensuring that the protective component 3 is connected to the culture component 1 when it is used. During the process, it can be effectively protected, and the protection component 3 is provided, on the one hand, it facilitates the stability of the culture component 1 during use, and on the other hand, it can ensure the safety of the culture component 1 during movement, and at least one vibration spring 4 is connected between the connecting cavity 103 and the protection component 3. When multiple vibration springs 4 are provided, the multiple vibration springs 4 are distributed between the connecting cavity 103 and the protection component 3 in a circular array with the fixed sleeve 201 as the center. In this solution, there are two vibration springs 4 between the connecting cavity 103 and the protection component 3. When the solution in the culture component 1 needs to be mixed, the culture component 1 can be shaken at this time, so that the vibration spring 4 can be reciprocated and extended, so that the solution in the culture component 1 can be mixed evenly.

[0034] like Figure 1As shown, the connecting component 2 includes a fixed sleeve 201, which is used to limit the moving path of the movable rod 202. One end of the fixed sleeve 201 is fixedly connected to the bottom of the connecting cavity 103, so that the height of the fixed sleeve 201 is reduced by utilizing the depth of the connecting cavity 103, ensuring that when the user uses the culture dish component, its use height will not affect the user's operation and comfort during use, and a movable rod 202 is slidably provided at one end of the fixed sleeve 201 away from the culture component 1, wherein the movable rod 202 can be telescopically moved within the fixed sleeve 201, thereby realizing adjustment of the height of the connecting component 2, thereby realizing adjustment of the height and function of the protection component 3, wherein the movable rod 202 does not conflict with the fixed portion 304, thereby ensuring that when the protection component 3 is fixed, the culture component 1 can move.

[0035] like Figure 4 As shown, the protection component 3 includes a suction cup 301, and a connecting sleeve 302 is connected to one side of the suction cup 301. The mutual cooperation between the suction cup 301 and the connecting sleeve 302 can ensure the connection between the suction cup 301 and the connecting sleeve 302 and the smooth area, thereby ensuring that the protection component 3 can fix the position of the culture component 1 during use, reducing the number of times the user manually restrains the culture component 1 by holding it, and improving the user's operational convenience. The end of the connecting sleeve 302 away from the suction cup 301 is connected to a fixing portion 304, which strengthens the connection between the connecting sleeve 302 and the connecting component 2 through the fixing portion 304. The connection stability is ensured to ensure that the connecting sleeve 302 will not break during the use of the protection component 3. The fixing portion 304 is movably connected to the connecting component 2. After the protection component 3 fixes the position of the culture dish device, the movable connection between the fixing portion 304 and the connecting component 2 ensures that the vessel 101 can be angularly deflected. When the user operates the vessel 101, the angle of the vessel 101 can be adjusted according to different operating methods. After the protection component 3 fixes the position of the vessel 101, the operation convenience of the culture dish device is improved by changing the angle of the vessel 101.

[0036] During use, the protective component 3 can not only ensure its stable position by adsorption with the ground, but also be turned over so that the suction cup 301 wraps the position of the container 101 in the opposite direction, and the connecting sleeve 302 covers the bottom of the container 101, thereby improving the safety of the container 101 during use and reducing the possibility of damage to the container 101 due to collision.

[0037] It should be noted that the smooth area where the suction cup 301 and the connecting sleeve 302 are connected includes but is not limited to a clean desktop, glass, or other flat surfaces that can be adsorbed by the protective component 3 .

[0038] Furthermore, the culture component 1 includes a vessel 101, which supports mesenchymal stem cells. The connecting cavity 103 is located at the end of the vessel 101 away from the opening, and the end of the vessel 101 away from the connecting cavity 103 is connected to a reinforcement ring 102. The opening direction of the vessel 101 is reinforced by the reinforcement ring 102, thereby reducing the risk of debris falling into the vessel 101 during friction of the culture component 1, affecting the cultivation of cells. At the same time, the setting of the connecting cavity 103 does not affect the interior of the vessel 101, thereby ensuring that the vessel 101 can meet the requirements for the cultivation of mesenchymal stem cells.

[0039] It should be noted that when the vessel 101 is flipped to the maximum angle, the solution of standard depth inside the vessel 101 will not spill, that is, the wall height of the vessel 101 and the elastic change of the vibration spring 4 correspond to each other, ensuring that during the process of vibrating the vessel 101 using the vibration spring 4, the vessel 101 will not leak the solution inside it.

[0040] like Figure 4 As shown, further, in order to ensure that the protection component 3 can be used normally and also ensure the service life of the protection component 3, the suction cup 301, the connecting sleeve 302 and the fixing portion 304 are all made of silicone material, thereby ensuring that the protection component 3 can be effectively connected to the smooth surface, and the fixing portion 304 is an annular body, ensuring that the connection between the fixing portion 304 and the connecting component 2 is fixed, and at the same time, by setting the fixing portion 304 into a ring, when it is necessary to cancel the position fixation of the protection component 3, the suction cup 301 and the connecting sleeve 302 are inflated through the inner ring of the fixing portion 304, thereby reducing the adsorption force between the protection component 3 and the smooth surface, so as to contact the fixation of the protection component 3, and realize Now let's move the culture component 1, where the diameter of the inner ring of the fixing part 304 close to the culture component 1 is larger than the diameter of the fixing part 304 away from the culture component 1, thereby ensuring that when the protection component 3 is adsorbed and fixed, the movable plate 204 can seal the inner ring of the fixing part 304, ensuring that a sealed cavity can be formed when the suction cup 301, the connecting sleeve 302 and the fixing part 304 are in contact with the smooth plane, thereby fixing the position of the protection component 3. When the protection component 3 needs to cancel the restriction on the smooth plane, by moving the movable plate 204, the external gas can enter the protection component 3 through the inner ring of the fixing part 304, so as to cancel the position fixation of the culture dish equipment by the protection component 3.

[0041] like Figure 6As shown, further, in order to quickly realize the fixing and canceling of the protection component 3, the connecting component 2 also includes a movable plate 204, wherein the movable plate 204 is a colloid material, and the movable plate 204 is movably connected to the inner ring of the fixed part 304, that is, the movable plate 204 can slide in the inner ring of the fixed part 304, and at the same time, the movable plate 204 can be clamped on the inner ring of the fixed part 304, so that the inner ring of the fixed part 304 forms a sealing state, and the movable plate 204 conflicts with the inner ring of the fixed part 304, and the movable plate 204 will not be separated from the fixed part 304, and then the closing and opening of the inner ring of the fixed part 304 are realized by the movement of the movable plate 204.

[0042] like Figure 6 As shown, further, in order to facilitate the adjustment of the angle of the culture component 1 after the position of the protection component 3 is fixed, and when necessary, the angle of the vessel 101 can also be adjusted according to the elastic change of the vibration spring 4, the connection component 2 further includes a ball joint 203, and the connection between the movable rod 202 and the protection component 3 is rotatably connected through the connection of the ball joint 203. The fixed end of the ball joint 203 is connected to one end of the movable rod 202, and the movable end of the ball joint 203 is connected to the movable plate 204. At the same time, the friction force of the ball joint 203 during rotation can be adjusted. The friction force of the ball joint 203 is thereby adjusted according to the use environment of the vessel 101 to ensure that the culture component 1 can meet the use requirements. That is, when the vessel 101 is in the process of culturing and injecting liquid, the friction force of the ball joint 203 is relatively large, so that the vessel 101 will not rotate during use. When the cells in the vessel 101 need to be mixed, the friction force of the ball joint 203 is adjusted to be relatively small, so that when the vibration spring 4 expands and contracts, it can effectively drive the vessel 101 to shake, ensuring that the solution is in full contact with the mesenchymal stem cells.

[0043] The friction force adjustment method of the ball joint 203 is preferably knob control, and the friction force of the ball joint 203 is changed by controlling the change of the resistance force between the knob and the ball joint 203, thereby improving the adjustment efficiency of the friction force of the ball joint 203, thereby meeting the use requirements of the culture dish equipment. The adjustment method is existing technology and the process will not be described in detail here.

[0044] like Figure 8-Figure 9As shown, further, in order to facilitate the stacking of multiple culture dish devices during storage, reduce space occupancy and improve stacking stability, a docking sleeve 303 is connected to the end of the suction cup 301 away from the connecting sleeve 302, and a docking groove 3021 is provided on the inner side of the connecting sleeve 302, which is close to the connection position between the connecting sleeve 302 and the suction cup 301, wherein the docking sleeve 303 is adapted to the docking groove 3021, and then when the suction cup 301 covers the edge of the vessel 101, when multiple culture dish devices need to be stacked for culture, the docking groove 3021 on one culture dish device can correspond to the docking sleeve 303 on another culture dish device, so that the docking sleeve 303 is embedded in the docking groove 3021, and so on, thereby ensuring that the multiple culture dish devices are docked with each other, and when the culture dish devices are stacked, their bottoms are the connecting sleeve 302, which have an anti-slip effect when in contact with a flat surface.

[0045] like Figure 5-Figure 7 As shown, further, in order to facilitate the suction cup 301 to wrap the circumferential wall of the vessel 101, a fold is provided at the connection position between the connecting sleeve 302 and the suction cup 301, and the fold will not interfere with the adsorption of the protective component 3. At the same time, when the suction cup 301 and the connecting sleeve 302 are folded, the fold setting can prompt the connecting sleeve 302 to be folded to the bottom edge of the vessel 101, and the suction cup 301 wraps the edge of the vessel 101, thereby improving the folding efficiency.

[0046] like Figure 6 As shown, further, in order to facilitate the state change adjustment of the protection component 3, a movable cavity is opened in the fixed sleeve 201, and an elastic reset device is connected to the movable cavity, and the elastic reset device is in conflict with the movable rod 202. One end of the vibration spring 4 is connected to the cavity wall of the connecting cavity 103, and the other end of the vibration spring 4 is connected to the connecting sleeve 302 to ensure that when the connecting sleeve 302 and the suction cup 301 cover the vessel 101, the movable rod 202 can be retracted into the fixed sleeve 201 without interfering with the connection position of the connecting sleeve 302. At the same time, when the protection component 3 is restored, when the suction cup 301 is about to be separated from the vessel 101, the elastic reset device can be used to reset the connecting sleeve 302.

[0047] During operation, the culture dish device is first moved to the target area. At this time, the friction force of the ball joint 203 is relatively large to maintain the angle between the connecting component 2 and the protective component 3 from changing, ensuring the stable movement of the culture component 1. When the culture dish device is in the target position, the protective component 3 is brought into contact with and pressed against the target area, prompting the suction cup 301 and the connecting sleeve 302 to fully contact and adsorb the target area. In this process, the movable plate 204 blocks the inner ring of the fixed part 304, thereby fixing the position of the culture component 1, making it convenient for the operator to operate and process the substances in the vessel 101.

[0048] When the solution inside the vessel 101 needs to be adsorbed and cleaned, the friction force of the ball joint 203 is maintained unchanged. The angle of the vessel 101 is adjusted and fixed by the user to adjust the angle of the vessel 101 so that the solution in the vessel 101 is gathered to one side, thereby reducing the time the user holds the vessel 101, improving the user's operability, and facilitating the absorption or addition of the solution in the vessel 101. After the solution in the vessel 101 is cleaned, new solution needs to be added to the vessel 101 to flush the cells. Wash and cultivate, and then in the process of filling the liquid, maintain the tilt angle of the vessel 101 unchanged to complete the filling, and after the vessel 101 is filled with liquid, reduce the friction of the ball joint 203 by turning the knob, and then lightly touch the edge of the vessel 101, so that the vessel 101 shakes, thereby driving the vibration spring 4 to shake, and utilizing the reciprocating elastic change of the vibration spring 4 to cause the vessel 101 to shake, so as to promote the uniform mixing of the solution in the vessel 101, reduce the time consumed by manually shaking the vessel 101, and improve the use efficiency of the vessel 101.

[0049] After the operation on the cells in the vessel 101 is completed, the friction force of the ball joint 203 is increased to ensure that the vessel 101 can maintain a relatively static state with the suction cup 301. At the same time, the user pulls the vessel 101 toward the end away from the plane, thereby driving the vessel 101 and the connecting component 2 to move toward the end away from the protective component 3, thereby driving the movable plate 204 to move within the inner ring of the fixed part 304, releasing the blockage of the movable plate 204 on the protective component 3, allowing gas to enter the protective component 3, thereby prompting the protective component 3 to release the connection with the plane, making it easier for the user to move the culture dish equipment.

[0050] It should be noted that when the ball joint 203 rotates, the ball joint 203 only rotates and does not cause relative movement between the movable plate 204 and the fixed portion 304 .

[0051] At the same time, when the culture dish device enters the culture stage, the user folds the protection component 3, and at the same time compresses and limits the vibration spring 4 and the movable rod 202, so that the connecting sleeve 302 covers the bottom of the vessel 101, and then the suction cup 301 covers the edge of the vessel 101, thereby achieving full coverage of the vessel 101, protecting the vessel 101 from the risk of collision, and ensuring that the vessel 101 will not be damaged during the cultivation process.

[0052] After the protective component 3 covers the edge of the vessel 101, the docking groove 3021 is located at the bottom of the vessel 101, and the docking sleeve 303 is located at the mouth of the vessel 101. Therefore, when multiple vessels 101 are stacked, the docking sleeve 303 and the docking groove 3021 can correspond to each other, ensuring that the position of the stacked vessels 101 is stable.

[0053] By cooperating with each other, the protective component 3 and the connecting component 2 can fix the position of the vessel 101, thereby facilitating the use of the vessel 101 by the user and reducing the discomfort caused by operating the vessel 101. The rotation angle of the vessel 101 can also be increased, so that the user can enhance the performance of the vessel 101. At the same time, the protective component 3 and the connecting component 2 cooperate with each other to assist the user in evenly mixing the solution in the vessel 101 and fully contacting the solution with the cells inside the vessel 101, thereby improving the culture efficiency of the culture component 1.

[0054] It should be noted that the terms "comprises," "includes," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements, but also other elements not expressly listed, or elements that are inherent to such process, method, article, or apparatus.

[0055] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A culture dish device for collecting mesenchymal stem cell factors, comprising a culture component (1), characterized in that: A connecting cavity (103) is provided at one end of the culture component (1), a connecting component (2) is provided in the connecting cavity (103), a protective component (3) is connected to the end of the connecting component (2) away from the culture component (1), and at least one vibration spring (4) is connected between the connecting cavity (103) and the protective component (3); The connecting assembly (2) comprises a fixed sleeve (201), one end of the fixed sleeve (201) is fixedly connected to the connecting cavity (103), and an end of the fixed sleeve (201) away from the culture assembly (1) is slidably provided with a movable rod (202); The protection component (3) comprises a suction cup (301), a connecting sleeve (302) is connected to one side of the suction cup (301), a fixing portion (304) is connected to one end of the connecting sleeve (302) away from the suction cup (301), and the fixing portion (304) is movably connected to the connecting component (2); The suction cup (301), the connecting sleeve (302) and the fixing portion (304) are all made of silicone material, and the fixing portion (304) is an annular body, wherein the diameter of the inner ring of the fixing portion (304) close to the culture component (1) is larger than the diameter of the fixing portion (304) away from the culture component (1); The connecting assembly (2) further comprises a movable plate (204), the movable plate (204) being movably connected to the inner ring of the fixed portion (304), the movable plate (204) being in contact with the inner ring of the fixed portion (304), and the movable plate (204) will not be separated from the fixed portion (304); The connecting assembly (2) further comprises a ball joint (203), wherein the fixed end of the ball joint (203) is connected to one end of the movable rod (202), and the movable end of the ball joint (203) is connected to the movable plate (204); The protection component (3) can utilize the suction cup (301) and the connecting sleeve (302) to reversely wrap the culture component (1), thereby improving the safety of the culture component (1) during use.

2. The culture dish device for collecting mesenchymal stem cell factors according to claim 1, characterized in that: The culture assembly (1) comprises a vessel (101), the connecting cavity (103) is located at one end of the vessel (101) away from the opening, and a reinforcing ring (102) is connected to the end of the vessel (101) away from the connecting cavity (103).

3. The culture dish device for collecting mesenchymal stem cell factors according to claim 1, characterized in that: The end of the suction cup (301) away from the connecting sleeve (302) is connected to a docking sleeve (303), and a docking groove (3021) is provided on the inner side of the connecting sleeve (302). The docking groove (3021) is close to the connection position between the connecting sleeve (302) and the suction cup (301), wherein the docking sleeve (303) is adapted to the docking groove (3021).

4. The culture dish device for collecting mesenchymal stem cell factors according to claim 3, characterized in that: A fold is provided at the connection position between the connecting sleeve (302) and the suction cup (301).

5. The culture dish device for collecting mesenchymal stem cell factors according to claim 1, characterized in that: A movable cavity is provided in the fixed sleeve (201), an elastic reset device is connected to the movable cavity, and the elastic reset device is in contact with the movable rod (202).

6. The culture dish device for collecting mesenchymal stem cell factors according to claim 2, characterized in that: One end of the vibration spring (4) is connected to the cavity wall of the connecting cavity (103), and the other end of the vibration spring (4) is connected to the connecting sleeve (302).

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