Pipetting system and immunity analyzer

Abstract

The invention relates to the field of in-vitro diagnostic equipment, and provides a pipetting system and an immunoassay analyzer, the pipetting system comprises a pipetting vertical driving assembly and a pipetting crevasse assembly, the pipetting vertical driving assembly comprises a first driving structure, a first ball screw structure, a first compression spring, a spring limiting sleeve, a nut adjusting mounting block, a guide adjusting pin, a first separation blade, a first sensor and a first mounting seat, and the first ball screw structure comprises a first screw rod, a first screw rod nut and a ball; the pipetting crevasse assembly comprises an injection mounting frame and an injection pump, and a pump head of the injection pump is used for loading a disposable TIP head. When the disposable TIP head is loaded, the loading force pressed on the disposable TIP head is buffered by the first compression spring, and the precise control of the loading force is completed through the cooperation of the first separation blade and the first sensor, so that the technical problem that in the prior art, the loading is unsuccessful due to the too small loading force or the disposable TIP head is crushed due to the too large loading force is solved.

Description

Technical Field

[0001] The present invention relates to the field of in vitro diagnostic equipment, and in particular to a liquid transfer system and an immunoassay analyzer. Background Art

[0002] Fully automatic POCT (Point-of-Care Testing, POCT) high-precision immunoassay analyzers are widely used due to their efficient and convenient detection capabilities. When these devices perform sample analysis, the pipetting system in the device uses a disposable TIP head to transfer samples and reagents to ensure the hygiene of the test and avoid cross contamination. However, there are some technical challenges in the actual operation of existing fully automatic POCT immunoassay analyzers, especially in the process of loading the disposable TIP head on the pipetting system. The TIP head may fail to load successfully due to insufficient loading force or may be crushed by excessive loading force during loading, thus affecting the stability of the device.

[0003] Therefore, the prior art urgently needs to solve the problem of unsuccessful loading or extrusion damage of the TIP head during the loading process, so as to improve the reliability of the equipment, reduce the failure rate in operation, reduce maintenance costs, and improve overall work efficiency. Summary of the invention

[0004] The purpose of the present invention is to provide a liquid transfer system and an immunoassay analyzer, which solve the technical problem in the prior art that a disposable TIP head cannot be loaded successfully or is squeezed and damaged during the loading process.

[0005] The embodiment of the present invention provides a pipetting system, including: a pipetting vertical drive component, the pipetting vertical drive component includes a first drive structure, a first ball screw structure, a first compression spring, a spring limiting sleeve, a nut adjustment mounting block, a guide adjustment pin, a first baffle, a first sensor, and a first mounting seat; the first ball screw structure includes a first screw, a first screw nut and a ball, the first drive structure drives the first screw to rotate so as to drive the ball and the first screw nut to move, the nut adjustment mounting block is fixed to the first screw nut, the nut adjustment mounting block is provided with a first through hole, and the pin head of the guide adjustment pin is clamped in the first through hole. Above the first through hole, the pin of the guide adjustment pin passes through the first through hole and is fixed to the first mounting seat, the guide adjustment pin can move relative to the nut adjustment mounting block, the first compression spring is located between the spring limiting sleeve and the first screw nut, and the distance difference between the first baffle and the first sensor is less than or equal to the original gap between the first mounting seat and the nut adjustment mounting block; a pipetting breakout assembly, the pipetting breakout assembly includes an injection mounting frame and an injection pump, the injection mounting frame is connected to the first mounting seat, the injection pump is fixed to the injection mounting frame, and the pump head of the injection pump is used to load a disposable TIP head.

[0006] Furthermore, the first compression spring is a spring with increasing pitch.

[0007] Furthermore, the pipetting vertical drive assembly also includes a linear guide rail, a linear slider and a guide rail mounting frame, the linear guide rail is parallel to the first screw rod and is arranged on one side of the guide rail mounting frame, and the linear slider is connected to the first mounting seat.

[0008] Furthermore, the pipetting vertical drive assembly also includes a second sensor, and the second sensor is installed on a side of the guide rail mounting frame close to the first baffle.

[0009] Furthermore, the pipetting vertical drive assembly also includes a sliding pin sleeve, which is arranged in the first through hole, and the pin column of the guide adjustment pin passes through the sliding pin sleeve.

[0010] Furthermore, the pipetting vertical drive assembly also includes a first motor fixing plate, the first drive structure is installed on the first motor fixing plate, the first drive structure includes a first drive motor, a first active synchronous wheel, a first synchronous belt and a first driven synchronous wheel, the first drive motor is connected to the first active synchronous wheel, and the first driven synchronous wheel is connected to the first screw rod.

[0011] Furthermore, the pipetting vertical drive assembly also includes a first flange bearing, a first flange bearing steel sleeve and a nut gasket, the first screw rod passes through the first flange bearing, the first flange bearing steel sleeve is embedded in the first motor fixing plate, and the nut gasket is located between the first driven synchronous wheel and the first flange bearing.

[0012] Furthermore, the liquid transfer breaking assembly also includes a cutting knife fixing seat and a breaking cutting knife, the cutting knife fixing seat is connected to the pump body of the injection pump, and the breaking cutting knife is fixed below the cutting knife fixing seat.

[0013] Furthermore, the pipetting rupture assembly also includes a spring pressure head, a second compression spring and a rupture fixing column, the cutting knife fixing seat includes a fixing hole, one end of the rupture fixing column is stuck in the fixing hole, the second compression spring is located in the fixing hole, and the spring pressure head is pressed above the second compression spring and fixed in the fixing hole.

[0014] Furthermore, it also includes a suction and discharge drive component, which includes a second mounting seat, a second motor fixing plate, a second driving structure, a second ball screw structure and an injection drive block, the second ball screw structure includes a second screw, a second screw nut and a ball, the second mounting seat is connected to the first mounting seat, the second motor fixing plate is installed on the second mounting seat, the second driving structure is installed on the second motor fixing plate, the two ends of the second screw are respectively connected to the second driving structure and the bottom plate of the second mounting seat, the second driving structure drives the second screw to rotate and drives the second screw nut to move, and the injection drive block is fixed to the second screw nut.

[0015] Furthermore, the pipetting break assembly also includes a TIP head removal structure, which includes a removal guide rod, a third compression spring, a removal plate and a limiting pressure block. The two ends of the removal guide rod respectively pass through the injection mounting bracket and the cutting knife fixing seat, one end of the removal guide rod is connected to the limiting pressure block, the third compression spring is sleeved on the removal guide rod and is located between the limiting pressure block and the injection mounting bracket, and the other end of the removal guide rod is connected to the removal plate.

[0016] Furthermore, the suction and discharge drive assembly also includes a second baffle, a third sensor and a fourth sensor, the second baffle is connected to the injection drive block, and the third sensor and the fourth sensor are connected to the side plate of the second mounting seat.

[0017] Furthermore, the second driving structure includes a second driving motor, a second active synchronous wheel, a second synchronous belt, and a second driven synchronous wheel. The second driving motor is connected to the second active synchronous wheel, and the second driven synchronous wheel is connected to the second screw rod.

[0018] Furthermore, the suction and discharge drive assembly also includes a linear guide rod, a linear bearing and a buffer sleeve. The linear guide rod is arranged between the second motor fixing plate and the bottom plate of the second mounting seat. The linear bearing is fixed to the injection drive block. The linear guide rod passes through the linear bearing. The buffer sleeve is fixed on the side of the linear guide rod close to the second motor fixing plate.

[0019] Furthermore, the pipetting system also includes a magnetic separation and cleaning component, which is located below the pipetting vertical drive component.

[0020] In a second aspect, an embodiment of the present invention provides an immunoassay analyzer, comprising the pipetting system as described in the preceding item.

[0021] The embodiments of the present invention have at least the following technical effects:

[0022] An embodiment of the present invention provides a pipetting system, in which a first compression spring generates an initial upward pre-tightening force on a first screw nut, and the first screw nut transmits the force to the first screw via a ball, so that the first ball screw structure is no longer in a relaxed state, and the upper curved surface of the first screw and the lower curved surface of the first screw nut, with which the ball is always in contact, are in close contact; even if the rotation direction of the first screw changes, due to the initial pre-pressure of the first compression spring, the first ball screw structure is still in a non-relaxed state, thereby eliminating the influence of the backlash and ensuring the displacement accuracy when the pipetting vertical drive assembly drives the pipetting breach assembly to move up and down.

[0023] The first screw nut and the nut adjustment mounting block are locked together by a nut, the pin head of the guide adjustment pin is stuck above the nut adjustment mounting block, and the pin column of the guide adjustment pin is also fixed to the first mounting seat by a screw thread. The first screw nut blocks the first compression spring, and the downward spring force of the first compression spring is transmitted to the upper flange retaining edge of the spring limiting sleeve to make it stuck on the first mounting seat, which is equivalent to the spring limiting sleeve being fixed to the first mounting seat.

[0024] When the disposable TIP head is not loaded, the first compression spring, spring limit sleeve, nut adjustment mounting block, guide adjustment pin, first mounting seat and first screw nut move up and down within the effective stroke of the first screw as a whole, so the first compression spring does not change, and the gap between the nut adjustment mounting block and the first mounting seat does not change. During the loading process, as the loading force gradually increases, the upward force of the disposable TIP head on the pump head also increases, and the gap between the nut adjustment mounting block and the first mounting seat also decreases. The first mounting seat and the spring limit sleeve have an upward displacement relative to the first screw nut, and the deformation of the first compression spring also increases, becoming a more compressed state. Therefore, the loading force can be accurately controlled by controlling the deformation of the first compression spring.

[0025] There is a small distance difference between the first baffle and the detection position of the first sensor during initial installation. The deformation of the first compression spring is the distance difference between the first baffle and the first sensor. The distance between the first baffle and the first sensor should be less than or equal to the original gap between the nut adjustment mounting block and the first mounting seat so that the first baffle and the first sensor can cooperate to effectively control the compression amount of the first compression spring and thus control the downward loading force.

[0026] In this embodiment, when loading the disposable TIP head, the first screw nut drives the first compression spring, the spring limiting sleeve, the nut adjustment mounting block, the guide adjustment pin, the first mounting seat and the injection pump of the pipetting rupture assembly to move downward. When the pump head of the injection pump contacts the disposable head, the first screw nut continues to move downward, and the loading force pressed on the disposable head is buffered by the first compression spring. The first baffle and the first sensor cooperate to complete the precise control of the loading force, ensuring that when loading the disposable TIP head, the loading will not fail due to too small a loading force or the disposable TIP head will be crushed due to too large a loading force. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0028] Figure 1 A schematic diagram of the three-dimensional structure of a liquid transfer system provided in an embodiment of the present invention;

[0029] Figure 2 A schematic diagram of a pipetting vertical drive assembly of a pipetting system provided in an embodiment of the present invention;

[0030] Figure 3 A cross-sectional schematic diagram of a liquid transfer vertical drive assembly of a liquid transfer system provided in an embodiment of the present invention;

[0031] Figure 4 for Figure 3 A is an enlarged schematic diagram;

[0032] Figure 5 A partial structural plan view of a liquid transfer vertical drive assembly of a liquid transfer system provided in an embodiment of the present invention;

[0033] Figure 6 A schematic diagram of the relationship between load and compression stroke of a first compression spring of a pipetting system provided in an embodiment of the present invention;

[0034] Figure 7 A schematic diagram of a pipetting break assembly of a pipetting system provided in an embodiment of the present invention;

[0035] Figure 8 A schematic diagram of a liquid suction and discharge drive assembly of a liquid transfer system provided in an embodiment of the present invention;

[0036] Fig. 9 A cross-sectional schematic diagram of a liquid suction and discharge drive assembly of a liquid transfer system provided in an embodiment of the present invention;

[0037] Fig.10 A schematic diagram of a partial framework structure of an immunoassay analyzer provided in an embodiment of the present invention.

[0038] Icons: 1-pipette vertical drive assembly; 2-pipette break assembly; 3-suction and discharge drive assembly; 4-magnetic separation and cleaning assembly; 5-disposable TIP head; 10-pipette system; 20-incubation system; 30-substrate filling system; 40-detection system; 101-first drive structure; 102-first ball screw structure; 103-first compression spring; 104-spring limit sleeve; 105-screw nut adjustment mounting block; 106-guide adjustment pin; 107-first baffle; 108-first transmission sensor; 109-first mounting seat; 110-linear guide rail; 111-linear slider; 112-guide rail mounting frame; 113-second sensor; 114-sliding pin sleeve; 115-first motor fixing plate; 116-first flange bearing; 117-first flange bearing steel sleeve; 118-first nut gasket; 119-pipette assembly connecting plate; 201-injection mounting frame; 202-injection pump; 203-cutter fixing seat; 204-breaking cutter; 205-spring pressure head; 206-second Compression spring; 207-break fixing column; 301-second mounting seat; 302-second motor fixing plate; 305-injection drive block; 306-second baffle; 307-third sensor; 308-fourth sensor; 309-linear guide rod; 310-linear bearing; 311-buffer sleeve; 312-second flange bearing; 313-second flange bearing steel sleeve; 314-second nut gasket; 315-second deep groove ball bearing; 1011-first drive motor; 1012-first active synchronous Step wheel; 1013-first synchronous belt; 1014-first driven synchronous wheel; 1021-first screw rod; 1022-first screw rod nut; 1023-ball; 2081-removal guide rod; 2082-third compression spring; 2083-removal plate; 2084-limiting pressure block; 3041-second screw rod; 3042-second screw rod nut; 3031-second drive motor; 3032-second active synchronous wheel; 3033-second synchronous belt; 3034-second driven synchronous wheel. DETAILED DESCRIPTION

[0039] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] It will be understood by those skilled in the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as generally understood by those skilled in the art in the art to which the present invention belongs. It should also be understood that terms such as those defined in general dictionaries should be understood to have meanings consistent with the meanings in the context of the prior art, and will not be interpreted with idealized or overly formal meanings unless specifically defined as herein.

[0041] It will be understood by those skilled in the art that, unless otherwise stated, the singular forms "a", "an", "said" and "the" used herein may also include plural forms. It should be further understood that the term "comprising" used in the specification of the present invention refers to the presence of the features, integers, steps, operations, elements and / or components, but does not exclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or combinations thereof. The term "and / or" used herein includes all or any unit and all combinations of one or more associated listed items.

[0042] For the first aspect, see Figures 1 to 10 The embodiment of the present invention provides a pipetting system 10, comprising: a pipetting vertical drive assembly 1, the pipetting vertical drive assembly 1 comprising a first drive structure 101, a first ball screw structure 102, a first compression spring 103, a spring limiting sleeve 104, a nut adjustment mounting block 105, a guide adjustment pin 106, a first baffle 107, a first sensor 108, and a first mounting seat 109;

[0043] The first ball screw structure 102 includes a first screw 1021, a first screw nut 1022 and a ball 1023. The first driving structure 101 drives the first screw 1021 to rotate, thereby driving the ball 1023 and the first screw nut 1022 to move. The nut adjustment mounting block 105 is fixed to the first screw nut 1022. The nut adjustment mounting block 105 is provided with a first through hole. The pin head of the guide adjustment pin 106 is stuck above the first through hole. The pin column of the guide adjustment pin 106 passes through the first through hole and is fixed to the first mounting seat 109. The guide adjustment pin 106 can move relative to the nut adjustment mounting block 105. The first compression spring 103 is located between the spring limiting sleeve 104 and the first screw nut 1022. The distance difference X between the first baffle 107 and the first sensor 108 is less than or equal to the original gap D between the nut adjustment mounting block 105 and the first mounting seat 109.

[0044] The pipetting breakout assembly 2 includes an injection mounting frame 201 and an injection pump 202. The injection mounting frame 201 is connected to the first mounting seat 109. The injection pump 202 is fixed to the injection mounting frame 201. The pump head of the injection pump 202 is used to load a disposable TIP head.

[0045] In this embodiment, for a general ball screw structure, under normal circumstances, there will be a certain gap between the screw, steel ball and nut at the microscopic level, and they are in a relaxed state. As the screw rotates, the screw will drive the steel ball and thus drive the nut to move. At this time, the steel ball will be tightly attached to one side of the screw and nut to transmit the movement, but when the rotation direction of the screw changes, the force direction of the screw and nut changes, so the steel ball will be tightly attached to the other side of the screw and nut. Due to the gap between the screw, nut and steel ball, an axial error of the size of the gap difference will be generated during axial movement, which is the backlash of the screw nut. In the present application, the first compression spring 103 will generate an initial upward pre-tightening force on the first screw nut 1022, and the first screw nut 1022 transmits the force to the first screw 1021 through the ball 1023, so that the first ball screw structure 102 is no longer in a relaxed state, and the ball 1023 is always in close contact with the upper curved surface of the first screw 1021 and the lower curved surface of the first screw nut 1022. Even if the rotation direction of the first screw 1021 changes, due to the initial pre-pressure of the first compression spring 103, the first ball screw structure 102 is still in a non-relaxed state, eliminating the influence of the backlash and ensuring the displacement accuracy when the pipette vertical drive component 1 drives the pipette breach component 2 to move up and down.

[0046] The first screw nut 1022 is locked with the nut adjustment mounting block 105 through a nut, the pin head of the guide adjustment pin 106 is stuck above the nut adjustment mounting block 105, and the pin of the guide adjustment pin 106 is also fixed to the first mounting seat 109 through a screw thread. The first screw nut 1022 blocks the first compression spring 103, and the downward spring force of the first compression spring 103 is transmitted to the upper flange rib of the spring limiting sleeve 104 to make it stuck on the first mounting seat 109. The effect here is equivalent to the spring limiting sleeve 104 being fixed with the first mounting seat 109. Here, in order to facilitate assembly, the spring limiting sleeve 104 and the first mounting seat 109 are designed into such a structure. For example, in other embodiments, the spring limiting sleeve 104 and the first mounting seat 109 can also be fixed together with screws, as long as the effect of fixing them together is sufficient.

[0047] When the disposable TIP head is not loaded, the first compression spring 103, the spring limiting sleeve 104, the nut adjustment mounting block 105, the guide adjustment pin 106, the first mounting seat 109 and the first screw nut 1022 form a whole and move up and down within the effective stroke of the first screw 1021. Therefore, the first compression spring 103 will not change, and the gap between the nut adjustment mounting block 105 and the first mounting seat 109 will not change. The original gap size between the nut adjustment mounting block 105 and the first mounting seat 109 at the beginning of installation is D.

[0048] During the loading process, as the loading force gradually increases, the upward force of the disposable TIP head on the pump head also increases, and the gap between the nut adjustment mounting block 105 and the first mounting seat 109 will also decrease. The first mounting seat 109 and the spring limiting sleeve 104 have an upward displacement relative to the first screw nut 1022, and the deformation of the first compression spring 103 also increases, becoming a more compressed state. Therefore, the loading force can be accurately controlled by controlling the deformation of the first compression spring 103.

[0049] Figure 5The first baffle 107 is installed on the first mounting seat 109, and the first sensor 108 is installed on the screw nut adjustment mounting block 105. Of course, the first baffle 107 can also be installed on the spring limit sleeve 104, and the first sensor 108 can also be installed on the first screw nut 1022, or the first baffle 107 and the first sensor 108 can be swapped, because there is no relative displacement between the first mounting seat 109 and the spring limit sleeve 104, and there is no relative displacement between the screw nut adjustment mounting block 105 and the first screw nut 1022. There is a small distance difference X between the detection positions of the first baffle 107 and the first sensor 108 during the initial installation, so that the first baffle 107 triggers the first sensor 108 after the displacement of the distance X, and the deformation of the first compression spring 103 is the distance difference X between the first baffle 107 and the first sensor 108. The distance difference X here can also be adjusted continuously through experiments after the first baffle 107 and the first sensor 108 are fixed to obtain the best distance difference X. Therefore, the distance difference X between the first baffle 107 and the first sensor 108 should be less than or equal to the original gap D between the nut adjustment mounting block 105 and the first mounting seat 109, so that the first baffle 107 and the first sensor 108 can cooperate to effectively control the compression amount of the first compression spring 103 and thus control the downward loading force.

[0050] In this embodiment, when loading the disposable TIP head, the first screw nut 1022 drives the first compression spring 103, the spring limiting sleeve 104, the nut adjustment mounting block 105, the guide adjustment pin 106, the first mounting seat 109 and the injection pump 202 of the pipetting rupture assembly 2 to move downward. When the pump head of the injection pump 202 contacts the disposable TIP head, the first screw nut 1022 continues to move downward, and the loading force pressed on the disposable TIP head is buffered by the first compression spring 103. The first baffle 107 and the first sensor 108 cooperate to complete the precise control of the loading force, ensuring that when loading the disposable TIP head, the loading will not fail due to too small a loading force or the disposable TIP head will be crushed due to too large a loading force.

[0051] Optionally, the first compression spring 103 is a spring with increasing pitch. In this embodiment, in order to ensure that the loading force does not change suddenly and to ensure that the loading pressure is slowly pressed into the TIP head from small to large to complete the loading action, the first compression spring 103 selected here is a variable pitch spring with gradually increasing pitch. The stiffness k of the variable pitch spring gradually increases with the change of the spring deformation. The relationship curve between the load and the compression stroke of the variable pitch spring is as shown in Figure 6 As shown, the stiffness k of the ordinary constant pitch spring is constant, so the loading force changes quickly. The variable pitch spring is used to ensure that the loading pressure increases slowly, which can better avoid the loading force increasing too quickly and crushing the disposable TIP head.

[0052] Optionally, the liquid transfer vertical drive assembly 1 further includes a linear guide rail 110, a linear slider 111 and a guide rail mounting frame 112, wherein the linear guide rail 110 is parallel to the first screw rod 1021 and is disposed on one side of the guide rail mounting frame 112, and the linear slider 111 is connected to the first mounting seat 109. In this embodiment, the linear guide rail 110, the linear slider 111 and the guide rail mounting frame 112 are mainly used for guiding, so as to ensure that the first mounting seat 109 moves vertically up and down under the drive of the first screw rod nut 1022.

[0053] Optionally, the pipetting vertical drive assembly 1 further includes a second sensor 113, which is mounted on a side of the guide rail mounting frame 112 close to the first baffle 107. In this embodiment, the second sensor 113 is disposed on a side of the guide rail mounting frame 112 close to the first baffle 107, and the cooperation between the second sensor 113 and the first baffle 107 is to initialize positioning and upper limit position.

[0054] Optionally, the liquid transfer vertical drive assembly 1 further includes a sliding pin sleeve 114, which is disposed in the first through hole, and the pin of the guide adjustment pin 106 passes through the sliding pin sleeve 114. In this embodiment, the sliding pin sleeve 114 can reduce the friction force when the guide adjustment pin 106 moves relative to the nut adjustment mounting block 105, reduce the influence of the friction force when loading a disposable TIP head, and improve the control accuracy.

[0055] Optionally, the liquid transfer vertical drive assembly 1 further includes a first motor fixing plate 115, the first drive structure is mounted on the first motor fixing plate 115, the first drive structure 101 includes a first drive motor 1011, a first active synchronous wheel 1012, a first synchronous belt 1013 and a first driven synchronous wheel 1014, the first drive motor 1011 is connected to the first active synchronous wheel 1012, and the first driven synchronous wheel 1014 is connected to the first screw rod 1021. In this embodiment, the first drive motor 1011 drives the first active synchronous wheel 1012 to rotate, and the first driven synchronous wheel 1014 and the first screw rod 1021 are driven to rotate through the first synchronous belt 1013. This structure is simple and efficient, and a motor and other matching structures can also be used, as long as the first screw rod 1021 can be driven to rotate.

[0056] Optionally, the pipetting vertical drive assembly 1 further includes a first flange bearing 116, a first flange bearing steel sleeve 117 and a first nut washer 118, the first screw rod 1021 passes through the first flange bearing 116, the first flange bearing steel sleeve 117 is embedded in the first motor fixing plate 115, and the first nut washer 118 is located between the first driven synchronous wheel 1014 and the first flange bearing 116. In this embodiment, after a first flange bearing 116 is arranged at a position corresponding to the pipetting assembly connecting plate 119, the lower end of the first screw rod 1021 is sleeved therein; after two first flange bearings 116 and first flange bearing steel sleeves 117 are sleeved at the upper end of the first screw rod 1021, they are confined in the corresponding holes of the upper first motor fixing plate 115 to achieve the circular rotation of the screw rod. The two first flange bearings 116 here are used in conjunction with the first flange bearing steel sleeves 117. Adjusting the locking torque of the two first nut washers 118 during installation can reduce the axial clearance of the two first flange bearings 116, reduce the backlash error caused by the axial clearance of the bearings when the screw nut is rotated forward and reversely, and further improve the control accuracy of the up and down movement.

[0057] Optionally, the liquid transfer rupture assembly 2 further includes a cutter fixing seat 203 and a rupture cutter 204, the cutter fixing seat 203 is connected to the pump body of the injection pump 202, and the rupture cutter 204 is fixed below the cutter fixing seat 203. In this embodiment, before loading the disposable TIP head, the downward movement of the first screw nut 1022 also drives the cutter fixing seat 203 to move downward, and the rupture cutter 204 can cut the sealing film on the reagent strip, which is convenient for the subsequent loading of the disposable TIP head and adding the liquid in the reagent strip according to the test items. Figure 7 The six rupture cutting knives 204 are arranged corresponding to the number of injection pumps, and the number can be increased or decreased according to actual needs.

[0058] Optionally, the liquid transfer rupture assembly 2 further includes a spring pressure head 205, a second compression spring 206 and a rupture fixing column 207, the cutter fixing seat 203 includes a fixing hole, one end of the rupture fixing column 207 is stuck in the fixing hole, the second compression spring 206 is located in the fixing hole, and the spring pressure head 205 is pressed above the second compression spring 206 and fixed in the fixing hole. In this embodiment, after the two rupture fixing columns 207 are respectively sleeved in the fixing holes on both sides of the cutter fixing seat 203, the two second compression springs 206 are respectively placed in the fixing holes, and then the spring pressure heads 205 are respectively screwed into the upper ends of the two fixing holes to press the second compression springs 206, and as the rupture cutter 204 gradually moves downward to perform the rupture action, the rupture fixing column 207 presses the component on which the reagent strip is placed, and the compression amount of the second compression spring 206 gradually increases, which plays a fixing role and a buffering role for the rupture cutter 204.

[0059] Optionally, the pipetting system 10 also includes a suction and discharge drive assembly 3, which includes a second mounting seat 301, a second motor fixing plate 302, a second driving structure, a second ball screw structure and an injection drive block 305, the second ball screw structure includes a second screw 3041, a second screw nut 3042 and a ball, the second mounting seat 301 is connected to the first mounting seat 109, the second motor fixing plate 302 is installed on the second mounting seat 301, the second driving structure is installed on the second motor fixing plate 302, the two ends of the second screw 3041 are respectively connected to the second driving structure and the bottom plate of the second mounting seat 301, the second driving structure drives the second screw 3041 to rotate and drives the second screw nut 3042 to move, and the injection drive block 305 is fixed to the second screw nut 3042.

[0060] In this embodiment, see Figure 7 and Figure 8 Six injection pumps 202 are installed side by side on the injection mounting frame 201. The upper end of the piston rod of the injection pump 202 is a flange convex edge, and the position of the piston rod corresponds one-to-one to the notch of the injection driving block 305, and the size of the notch is smaller than the diameter of the flange convex edge. When the second driving structure drives the second ball screw structure to drive the injection driving block 305 to move upward, it drives the piston rod of the injection pump 202 to move upward to complete the liquid suction action. When the injection driving block 305 moves downward, the piston rod of the injection pump 202 automatically returns to complete the liquid discharge action.

[0061] Optionally, the pipetting rupture assembly 2 also includes a TIP head removal structure, which includes a removal guide rod 2081, a third compression spring 2082, a removal plate 2083 and a limiting pressure block 2084. The two ends of the removal guide rod 2081 respectively pass through the injection mounting frame 201 and the cutting knife fixing seat 203, one end of the removal guide rod 2081 is connected to the limiting pressure block 2084, the third compression spring 2082 is sleeved on the removal guide rod 2081 and is located between the limiting pressure block 2084 and the injection mounting frame 201, and the other end of the removal guide rod 2081 is connected to the removal plate 2083.

[0062] In this embodiment, the TIP head removal structure is to automatically remove the disposable TIP head after use, so that the device can continue to work. The removal plate 2083 is connected to the two removal guide rods 2081, and the removal guide rods 2081 guide so that the removal plate 2083 can only move in the vertical direction; when the injection drive block 305 moves down and presses the limit pressure block 2084, it will drive the two removal guide rods 2081 and the removal plate 2083 to move down as a whole to complete the action of unloading the disposable TIP head. After unloading is completed, the injection drive block 305 moves up, and the two removal guide rods 2081 and the removal plate 2083 will move back to their positions under the action of the third compression spring 2082.

[0063] Optionally, the suction and discharge drive assembly 3 further includes a second baffle 306, a third sensor 307 and a fourth sensor 308, the second baffle 306 is connected to the injection drive block 305, and the third sensor 307 and the fourth sensor 308 are connected to the side plate of the second mounting seat 301. In this embodiment, the second baffle 306, the third sensor 307 and the fourth sensor 308 cooperate to initialize the displacement of the injection drive block 305 relative to the second mounting seat 301, and to position the upper limit and the lower limit.

[0064] Optionally, the second driving structure includes a second driving motor 3031, a second active synchronous wheel 3032, a second synchronous belt 3033, and a second driven synchronous wheel 3034. The second driving motor 3031 is connected to the second active synchronous wheel 3032, and the second driven synchronous wheel 3034 is connected to the second screw rod 3041. In this embodiment, a motor and a synchronous belt structure are used to drive the second screw rod 3041 to rotate. This structure is simple and efficient, and a motor and other matching structures can also be used as long as they can drive the second screw rod 3041 to rotate.

[0065] Optionally, the suction and discharge drive assembly 3 further includes a linear guide rod 309, a linear bearing 310 and a buffer sleeve 311. The linear guide rod 309 is disposed between the second motor fixing plate 302 and the bottom plate of the second mounting seat 301. The linear bearing 310 is fixed to the injection driving block 305. The linear guide rod 309 passes through the linear bearing 310. The buffer sleeve 311 is fixed to the side of the linear guide rod 309 close to the second motor fixing plate 302. In this embodiment, the linear guide rod 309 and the linear bearing 310 play a guiding role. The buffer sleeves 311 are respectively sleeved on the upper ends of the two linear guide rods 309 for buffering and collision prevention.

[0066] Optionally, the suction and discharge drive assembly 3 also includes a second flange bearing 312, a second flange bearing steel sleeve 313, a second nut gasket 314 and a second deep groove ball bearing 315, the second screw rod 3041 passes through the second flange bearing 312, the second flange bearing steel sleeve 313 is embedded in the second motor fixing plate 302, and the second nut gasket 314 is located between the second driven synchronous wheel 3034 and the second flange bearing 312.

[0067] In this embodiment, after a second deep groove ball bearing 315 is set at the position corresponding to the second mounting seat 301, the lower end of the second screw 3041 is sleeved therein; after two second flange bearings 312 and second flange bearing steel sleeves 313 are sleeved on the upper end of the second screw 3041, they are confined in the corresponding holes of the second motor fixing plate 302 above to achieve the circular rotation of the screw. The two second flange bearings 312 here are used in conjunction with the second flange bearing steel sleeves 313. When installing, adjusting the locking torque of the two second nut washers 314 can reduce the axial clearance of the two second flange bearings 312, reduce the backlash error caused by the axial clearance of the bearing when the screw nut is rotated forward and reversely, further improve the control accuracy of the up and down movement of the injection drive block 305, and better control the amount of suction and discharge.

[0068] Optionally, a magnetic separation and cleaning component 4 is also included, and the magnetic separation and cleaning component 4 is located below the pipetting vertical drive component 1. In this embodiment, the magnetic separation and cleaning component 4 is located below the pipetting component connecting plate 119, and performs magnetic separation and cleaning on the contents in the disposable TIP head.

[0069] In a second aspect, an embodiment of the present invention provides an immunoassay analyzer, see Fig.10 , including the pipetting system 10 as described in any of the preceding items, and also including an incubation system 20, a substrate filling system 30 and a detection system 40. The incubation system 20 can hold a disposable TIP head 5, a reagent strip and a reaction cup. The pipetting system 10 can load the disposable TIP head 5, absorb the reagent in the reagent strip, and put the liquid required for the final reaction into the reaction cup. The substrate filling system 30 is used to add the last substrate liquid to the reaction cup, and the detection system 40 detects the substance in the reaction cup.

[0070] Those skilled in the art will appreciate that the various operations, methods, steps, measures, and schemes discussed in the present invention may be alternated, modified, combined, or deleted. Further, other steps, measures, and schemes in the various operations, methods, and schemes discussed in the present invention may also be alternated, modified, rearranged, decomposed, combined, or deleted. Further, the steps, measures, and schemes in the prior art that are similar to those disclosed in the present invention may also be alternated, modified, rearranged, decomposed, combined, or deleted.

[0071] In the description of the present invention, it should be understood that the terms "center", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0072] The terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, unless otherwise specified, "plurality" means two or more.

[0073] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a direct connection, or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific conditions.

[0074] In the description of this specification, specific features, structures, materials or characteristics may be combined in an appropriate manner in any one or more embodiments or examples.

[0075] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A liquid transfer system, characterized in that: include: A pipetting vertical drive assembly, the pipetting vertical drive assembly comprising a first drive structure, a first ball screw structure, a first compression spring, a spring limit sleeve, a nut adjustment mounting block, a guide adjustment pin, a first baffle, a first sensor, and a first mounting seat; The first ball screw structure includes a first screw, a first screw nut and a ball, the first driving structure drives the first screw to rotate, thereby driving the ball and the first screw nut to move, the nut adjustment mounting block is fixed to the first screw nut, the nut adjustment mounting block is provided with a first through hole, the pin head of the guide adjustment pin is stuck above the first through hole, the pin column of the guide adjustment pin passes through the first through hole and is fixed to the first mounting seat, the guide adjustment pin can move relative to the nut adjustment mounting block, the first compression spring is located between the spring limiting sleeve and the first screw nut, and the distance difference between the first baffle and the first sensor is less than or equal to the original gap between the first mounting seat and the nut adjustment mounting block; The pipetting breakout assembly comprises an injection mounting frame and an injection pump, wherein the injection mounting frame is connected to the first mounting seat, the injection pump is fixed to the injection mounting frame, and the pump head of the injection pump is used to load a disposable TIP head.

2. The liquid transfer system according to claim 1, characterized in that: The first compression spring is a progressive pitch spring.

3. The liquid transfer system according to claim 1, characterized in that: The pipetting vertical drive assembly also includes a linear guide rail, a linear slider and a guide rail mounting frame. The linear guide rail is parallel to the first screw rod and is arranged on one side of the guide rail mounting frame. The linear slider is connected to the first mounting seat.

4. The liquid transfer system according to claim 3, characterized in that: The pipetting vertical drive assembly also includes a second sensor, which is mounted on a side of the guide rail mounting frame close to the first baffle.

5. The liquid transfer system according to claim 1, characterized in that: The pipetting vertical drive assembly further comprises a sliding pin sleeve, wherein the sliding pin sleeve is arranged in the first through hole, and the pin column of the guide adjustment pin passes through the sliding pin sleeve.

6. The liquid transfer system according to claim 1, characterized in that: The pipetting vertical drive assembly also includes a first motor fixing plate, the first drive structure is installed on the first motor fixing plate, the first drive structure includes a first drive motor, a first active synchronous wheel, a first synchronous belt and a first driven synchronous wheel, the first drive motor is connected to the first active synchronous wheel, and the first driven synchronous wheel is connected to the first screw rod.

7. The liquid transfer system according to claim 6, characterized in that: The pipetting vertical drive assembly also includes a first flange bearing, a first flange bearing steel sleeve and a first nut gasket. The first screw rod passes through the first flange bearing. The first flange bearing steel sleeve is embedded in the first motor fixing plate. The first nut gasket is located between the first driven synchronous wheel and the first flange bearing.

8. The liquid transfer system according to claim 1, characterized in that: The liquid transfer rupture assembly also includes a cutter fixing seat and a rupture cutter. The cutter fixing seat is connected to the pump body of the injection pump, and the rupture cutter is fixed below the cutter fixing seat.

9. The liquid transfer system according to claim 8, characterized in that: The pipetting rupture assembly also includes a spring pressure head, a second compression spring and a rupture fixing column. The cutting knife fixing seat includes a fixing hole. One end of the rupture fixing column is stuck in the fixing hole. The second compression spring is located in the fixing hole. The spring pressure head is pressed on top of the second compression spring and fixed in the fixing hole.

10. The liquid transfer system according to claim 8, characterized in that: The liquid suction and discharge drive assembly also includes a second mounting seat, a second motor fixing plate, a second driving structure, a second ball screw structure and an injection driving block. The second ball screw structure includes a second screw, a second screw nut and a ball. The second mounting seat is connected to the first mounting seat. The second motor fixing plate is installed on the second mounting seat. The second driving structure is installed on the second motor fixing plate. The two ends of the second screw are respectively connected to the second driving structure and the bottom plate of the second mounting seat. The second driving structure drives the second screw to rotate and drives the second screw nut to move. The injection driving block is fixed to the second screw nut.

11. The liquid transfer system according to claim 10, characterized in that: The pipetting break assembly also includes a TIP head removal structure, which includes a removal guide rod, a third compression spring, a removal plate and a limiting pressure block. The two ends of the removal guide rod respectively pass through the injection mounting bracket and the cutting knife fixing seat, one end of the removal guide rod is connected to the limiting pressure block, the third compression spring is sleeved on the removal guide rod and is located between the limiting pressure block and the injection mounting bracket, and the other end of the removal guide rod is connected to the removal plate.

12. The liquid transfer system according to claim 10, characterized in that: The suction and discharge drive assembly further includes a second baffle, a third sensor and a fourth sensor, the second baffle is connected to the injection drive block, and the third sensor and the fourth sensor are connected to the side plate of the second mounting seat.

13. The liquid transfer system according to claim 10, characterized in that: The second driving structure includes a second driving motor, a second active synchronous wheel, a second synchronous belt, and a second driven synchronous wheel. The second driving motor is connected to the second active synchronous wheel, and the second driven synchronous wheel is connected to the second screw rod.

14. The liquid transfer system according to claim 10, characterized in that: The suction and discharge liquid driving assembly also includes a linear guide rod, a linear bearing and a buffer sleeve. The linear guide rod is arranged between the second motor fixing plate and the bottom plate of the second mounting seat. The linear bearing is fixed to the injection driving block. The linear guide rod passes through the linear bearing. The buffer sleeve is fixed on one side of the linear guide rod close to the second motor fixing plate.

15. The liquid transfer system according to claim 1, characterized in that: It also includes a magnetic separation and cleaning component, which is located below the liquid transfer vertical driving component.

16. An immunoassay analyzer, characterized in that: The invention comprises a pipetting system as claimed in any one of claims 1 to 15.

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