Method for preventing and controlling chenopodium quinoa stem borer pest beet coracoid

Abstract

The invention belongs to the technical field of crop pest prevention and control, and particularly relates to a method for preventing and controlling chenopodium quinoa stem borer pest beet coracocystis, which specifically comprises the following steps: step S1, coating with a seed coating agent: washing with sterile water for 3 times, putting chenopodium quinoa seeds on sterile filter paper, and airing at room temperature for later use; taking a seed coating agent, adding a proper amount of sterile water into the seed coating agent, uniformly mixing to prepare medicine slurry, and adding the aired seeds for coating; s2, biological insecticide spraying: spraying the biological insecticide for the third time by using metarhizium anisopliae OD with 10 billion spores / g or spinetoram SC with the concentration of 60 g / L 20 days after quinoa seedling emergence and the seedling height of 12-20 cm, and spraying for the second time every 14 days, so as to realize prevention and control of the beet beak in the whole growth period of the quinoa; and spraying 10 billion spores / g metarhizium anisopliae OD or 60 g / L spinetoram SC for the third time in the chenopodium quinoa filling stage.

Description

Technical Field

[0001] The invention belongs to the technical field of crop pest control, and in particular relates to a method for controlling a quinoa stem-boring pest, the beet weevil. Background Art

[0002] Quinoa (Chenopod ium quinoa) is an annual dicotyledonous herbaceous plant of the genus Chenopodiaceae, originating in the Andes Mountains of South America. It is rich in nutrition and has the reputation of "super grain". In the 1990s, quinoa was introduced and planted in Tibet, my country, but it was not widely promoted due to serious pests and diseases. Since 2011, the large-scale planting and commercial development of quinoa have been carried out in Shanxi Province, Qinghai Province, Gansu Province, Inner Mongolia Autonomous Region, Hebei Province, Henan Province and Shandong Province in my country. It is one of the popular crops introduced and planted in recent years, and plays an important role in industrial structure adjustment and farmers' income increase and wealth. With the promotion of quinoa products, people gradually realize its nutritional value, and the demand for quinoa increases, and the planting area gradually increases therewith, but various insect pests appear in succession during its growth process, and the occurrence trend is aggravated year by year. The main quinoa pests reported are beet tube beetle, beet tortoise leaf beetle, yellow striped flea beetle, sedge toothed shin leaf beetle, leaf miner, horizontal striped cabbage stink bug, etc. Due to the frequent circulation and exchange of agricultural products in the market, many diseases and pests spread faster with agricultural products. The occurrence of grain diseases and pests has both regional specificity and generality. Only on the basis of understanding the local climate, soil and other environmental conditions can we grasp the laws of occurrence and development of diseases and pests, and take comprehensive prevention and control measures in time to effectively control diseases and pests.

[0003] The beet tube beetle, Lixus subtilis Boheman, belongs to the Coleoptera, Curculionidae, Cleoniinae, and Lixus tube beak. It is widely distributed in the quinoa-growing areas in northern my country, mainly in sugar beets, and harms some weeds in the Chenopodiaceae, Amaranthaceae, and Polygonaceae families. There are two main ways that the beet beetle causes damage to quinoa: first, the adults drill holes and lay eggs on the main stem and branches, forming small oval or diamond-shaped dark brown spots, which then swell into knots as the tissue proliferates, and the knots dry up and crack along the edges, allowing pathogens to enter and induce the disease, causing the leaves to wilt and the ears to rot and die; second, the larvae eat in the internal conductive tissues of the main stem and branches, creating tunnels and causing the conductive tissues to turn brown and necrotize, which not only seriously affects the plant's nutrient transport, but also often causes the main stem to break, the side branches to break, the seeds to be unfull or to form shriveled grains, resulting in a serious reduction in quinoa production. The stem-boring rate of this insect in quinoa-producing areas is 20% to 30%, and the serious plots reach 60%.

[0004] Due to the low proportion of insect-resistant germplasm resources in quinoa, its insect-resistant breeding work has been greatly restricted, resulting in insufficient resistance of the main sesame varieties promoted in production, the beet weevil. The beet weevil is difficult to detect in the early stages and difficult to control after the symptoms appear. At present, production mainly relies on chemical spraying for control, but there is a lack of high-efficiency agents and it cannot meet the requirements of green integrated prevention and control. There are few reports on the use and control effects of biological pesticides, and there is a lack of research on the use of agents to mix seeds to control quinoa pests in current production. Relevant scholars have only preliminarily explored chemical agents for the prevention and control of various quinoa pests, which can no longer meet the requirements of the current production of pollution-free agricultural products. In view of this, we proposed a method for the prevention and control of the quinoa stem borer, the beet weevil. Summary of the invention

[0005] The purpose of the present invention is to provide a method for controlling quinoa stem borer pest beetle weevil in order to solve the problems raised in the above-mentioned background technology.

[0006] The present invention provides a method for controlling quinoa stem borer pest beetle beetle, which specifically comprises the following steps:

[0007] Step S1: Seed coating: After rinsing with sterile water for 3 times, place the quinoa seeds on sterile filter paper, dry at room temperature, and set aside;

[0008] Take a seed coating agent, add an appropriate amount of sterile water to the seed coating agent and mix well to make a slurry, and add the dried seeds to coat them;

[0009] Step S2: Spraying with biological insecticides: Spraying with biological insecticides: 20 days after quinoa seedlings emerge, when the seedling height is 12 cm to 20 cm, use 10 billion spores / g of Metarhizium anisopliae OD or 60 g / L spinetoram SC for the third spraying, and conduct the second spraying after an interval of 14 days to achieve the prevention and control of beet beetles throughout the growth period of quinoa;

[0010] During the grain filling period of quinoa, 10 billion spores / g OD of Metarhizium anisopliae or 60 g / L ethyl spinetoram SC was used for the third spraying.

[0011] In the above technical solution, further, the method for screening the seed coating agent comprises:

[0012] Investigation of agronomic traits of quinoa seedlings: The pot method was used to determine the effects of seed coating agents on quinoa growth. 20 quinoa seedlings were randomly selected from each treatment plot 14 days after quinoa seedlings emerged. The plant height, root length, and fresh weight of above-ground and underground parts of quinoa were measured to evaluate the effects of the six agents on the agronomic traits of quinoa.

[0013] Indoor evaluation of the efficacy of seed dressing agents against Beet Weevil: On the 3rd, 7th, 14th, 21st and 28th days after quinoa emergence, the test seedlings were collected and the plants were moistened with moist cotton balls. Ten quinoa plants were placed in each insect pot, which was then placed in an insect pot with 10 adult Beet Weevil. The test insect sources were starved for 2 hours before the test treatment and then transferred to the experimental plants. The death of the adults was observed after 3 days, and the adult mortality rate was investigated.

[0014] In the above technical solution, further, the method for screening the seed coating agent also includes:

[0015] Field experiment. The specific method of the field experiment is as follows: the experiment is designed with 7 treatments: (1) chlorantraniliprole; (2) cyantraniliprole; (3) chlorantraniliprole·thiamethoxam; (4) bromothiocarb·thiamethoxam; (5) clothianidin; (6) thiamethoxam; (7) control. No treatment is given. Each treatment is repeated 4 times, with a total of 28 plots. The plots are arranged in random blocks. On the 7th, 14th, 21st and 28th days after emergence, the quinoa plants damaged by the beet beetle in each treatment plot are investigated, and the damage rate is calculated. Finally, the seed dressing agent is selected as follows: 10% chlorantraniliprole + 30% clothianidin or 20% cyantraniliprole + 20% thiamethoxam or 200g / L chlorantraniliprole.

[0016] In the above technical solution, further, the biopesticide spray screening method includes:

[0017] Through indoor toxicity determination, the number of deaths in each treatment is recorded and the relative toxicity index is calculated to screen biopesticides;

[0018] Field efficacy plot tests are used to verify the results of the pesticides screened out by indoor toxicity tests.

[0019] In the above technical scheme, further, the specific method for screening biological insecticides by indoor toxicity determination is: the indoor biological activity determination of beet tube beetle adults is carried out by leaf immersion method, repeated 3 times, and clean water is used as a control. Quinoa leaves of similar size are immersed in the liquid for 20s, taken out and dried naturally, and the leaves are placed in a culture dish with a diameter of 10 cm. 10 adults after starvation treatment for 24 hours are placed in each culture dish. The death situation is observed after 24 hours and 48 hours of application. According to the results of indoor biological activity determination of insecticides, 10 billion spores / g of Metarhizium anisopliae OD, 8000I U / μL Bacillus thuringiensis SC, 10 billion spores / mg Bacillus brevis SC, 60g / L spinetoram SC, each agent has 3 dose gradients, each dose is repeated 3 times, the number of eggs laid on the plants on the day of application and the number of egg-laying holes on the plants 3 days, 5 days, 7 days and 15 days after application are observed, and the control effect is judged according to the increase in the number of egg-laying holes;

[0020] Finally, the biological insecticides with better control effects were obtained: 60g / L spinetoram SC and 10 billion spores / g Metarhizium anisopliae OD.

[0021] In the above technical solution, further, the quinoa filling period is early August.

[0022] In the above technical solution, further, the dosage of 10% chlorantraniliprole + 30% clothianidin is 1200ml / 100kg seeds;

[0023] The dosage of 20% cyantraniliprole + 20% thiamethoxam is 450mL / 100kg seeds, and the dosage of 200g / L chlorantraniliprole is 900mL / 100kg seeds.

[0024] The beneficial effects of the present invention are:

[0025] 1. The method for controlling the quinoa stem-boring pest beetle weevil adopts seed coating and three biological pesticide sprayings to achieve control of the beetle weevil throughout the entire growth period of quinoa, effectively solving the problem of control of the beetle weevil.

[0026] 2. This method for controlling the quinoa stem-boring pest, the beet weevil, adopts indoor efficacy evaluation and field agent screening to screen seed dressing agents and biological pesticides for the control of quinoa beet weevil throughout the growing period, providing highly effective seed dressing agents and spray agents for the control of beet weevil, and through the coordinated application of seed dressing agents and spray fungicides, the application combination for efficient control of beet weevil throughout the growing period is clarified, providing theoretical guidance for efficient control of beet weevil throughout the growing period. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a category diagram of the test seed coating agent and biological pesticide of the present invention;

[0028] Figure 2 is a dendrogram showing the effects of the six seed dressing agents of the present invention on the number of beet tube beetle adults indoors;

[0029] Figure 3 This is a data graph showing the effect of the seed coating agent of the present invention on the mortality rate of adult beet beetles;

[0030] Figure 4 This is a data graph of the damage rate (%) of beet beetle beetles to seedlings 7 to 28 days after seedling emergence of the present invention;

[0031] Figure 5 is a field damage rate diagram of the beet tube beak weevil of the present invention;

[0032] Figure 6 This is a data diagram showing the efficacy of the seed coating agent of the present invention on the early stage quinoa beet beetle in the field;

[0033] Figure 7 It is a dendrogram of quinoa emergence rate of the present invention;

[0034] Figure 8 It is a dendrogram showing the effect of the seed coating agent treatment of the present invention on the plant height, root length, aboveground fresh weight and root weight of quinoa;

[0035] Fig. 9 This is a data graph showing the residual detection results of the seed coating agent of the present invention in quinoa grains;

[0036] Fig.10 This is a data diagram of indoor toxicity determination of the biological pesticides of the present invention on adult beet tube beetles;

[0037] Fig.11 This is a tree diagram of the efficacy of the various agents of the present invention on beet tube beetles in the field;

[0038] Fig.12 It is a data graph of the control effect of the same drug combination of the present invention on the beet beak weevil at the seedling stage, flowering stage and filling stage. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the present application to clearly describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments in the present application belong to the scope of protection of this application.

[0040] In the description of the present application, it should be noted that the terms used herein are only for describing specific embodiments, and are not intended to limit the exemplary embodiments according to the present application. For ease of description, the sizes of the various parts shown in the drawings are not drawn according to the actual proportional relationship. The technology, methods and equipment known to ordinary technicians in the relevant field may not be discussed in detail, but in appropriate cases, the technology, methods and equipment should be regarded as part of the authorization specification. In all examples shown and discussed here, any specific value should be interpreted as merely exemplary, rather than as a limitation. Therefore, other examples of the exemplary embodiments may have different values. It should be noted that similar numbers and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further discussed in subsequent drawings.

[0041] It should be noted that, in the present application, the terms "comprise", "include" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, an element defined by the sentence "comprises one..." does not exclude the presence of other identical elements in the process, method, article or device including the element. In addition, it should be noted that the scope of the method and device in the embodiment of the present application is not limited to performing functions in the order shown or discussed, and may also include performing functions in a substantially simultaneous manner or in reverse order according to the functions involved, for example, the described method may be performed in an order different from that described, and various steps may also be added, omitted, or combined. In addition, the features described with reference to certain examples may be combined in other examples.

[0042] Embodiment 1:

[0043] Effects of six seed coating agents on the control of the beet weevil

[0044] according to Figure 1-Figure 6 As shown in the figure, the indoor control efficacy evaluation test was designed with 7 treatments: (1) chlorantraniliprole (CHL); (2) cyantraniliprole (CYA); (3) chlorantraniliprole / thiamethoxam (CHL+CLO); (4) bromothiocarb / thiamethoxam (CYA+TH I); (5) clothianidin (CLO); (6) thiamethoxam (TH I); (7) control (CK), without any treatment. Each treatment was repeated 3 times. The six seed coating agents were applied one day before sowing. Figure 1 The corresponding coating doses were placed in sealed bags with 10g of quinoa seeds, the bags were tied tightly, and the seeds were shaken up and down to evenly cover the surface of the seeds. The seeds were placed on newspapers to dry for later use. 20 quinoa seeds were sown in a plastic flower pot (10cm in diameter, 20cm in height) containing nutrient soil, covered with a small amount of nutrient soil and placed at 25°C, with a light intensity of 16L:8D and a humidity of 60-70%. Pay attention to timely replenishment of water during seed germination and plant growth. On the 3rd, 7th, 14th, 21st and 28th days after quinoa germination, the test seedlings were collected, and the plants were moisturized with moist cotton balls. 10 quinoa plants were placed in each insect pot, and placed in an insect pot with 10 beet tube beetle adults. The test insect sources were starved for 2h before the test treatment and then transferred to the experimental plants. The death of adults was observed after 3d, and the adult mortality rate was investigated. The criterion for judging the death of adults is: use a wet brush to gently stimulate the body surface of the adult; if it cannot move normally, it is considered dead.

[0045] The field control efficacy test was conducted at the Science and Technology Innovation Base of Chengde Academy of Agriculture and Forestry Sciences. Quinoa was sown on May 27, 2023, with a sowing depth of 2.5 cm. Various agricultural operations were consistent during the quinoa growing season. The experiment was designed with 7 treatments: (1) chlorantraniliprole treatment (CHL); (2) cyantraniliprole treatment (CYA); (3) chlorantraniliprole·clothianidin (CHL+CLO); (4) bromothiocarb·thiamethoxam (CYA+THI); (5) clothianidin (CLO); (6) thiamethoxam (TH I); (7) control (CK), without any treatment, each treatment was repeated 4 times, with a total of 28 plots, and the plots were arranged in random blocks. On the 7th, 14th, 21st, 28th and 35th days after emergence, the quinoa plants damaged by the beet tube weevil in each treatment plot were investigated, and the damage rate was calculated. Since it is impossible to directly observe the death of beet beetles in the field, the damage rate of quinoa plants is used as the evaluation standard for prevention effectiveness. During the investigation, samples were taken at five diagonal points in each plot, and five quinoa plants were randomly selected at each point. The number of eggs laid on the seedlings on the same day was recorded starting from June 10, and the number of egg-laying holes on the plants at 7d, 14d, 21d, 28d, and 35d after the seedlings were investigated. The prevention effectiveness was judged based on the increase in the number of egg-laying holes. Relative prevention effectiveness = (the increase in the number of eggs laid in the control group - the increase in the number of eggs laid in the experimental area) / the increase in the number of eggs laid in the control group × 100%.

[0046] 1.1 Evaluation of the indoor control effect of seed coating agents on adults of beet tube weevils:

[0047] From the number of live insects in the room, it can be seen that the number of live insects after treatment with each agent was significantly lower than that of the control at each germination time. 3-28 days after germination, the number of live insects in the treatments of 20% cyantraniliprole + 20% thiamethoxam and 10% chlorantraniliprole + 30% clothianidin was 0.58-8.62, which was significantly lower than the number of live insects of 30% clothianidin, 30% thiamethoxam, 10% cyantraniliprole and 200g / L chlorantraniliprole, which was 0.81-9.28. There was no significant difference between the treatments of 200g / L chlorantraniliprole and 30% thiamethoxam.

[0048] From the mortality of beet beetles, it can be seen that the mortality of each treatment gradually decreased over time ( Figure 2). 3 days after emergence, the mortality of beet beetle under 6 treatments was higher than 85%, among which 10% chlorantraniliprole + 30% clothianidin, 20% cyantraniliprole + 20% thiamethoxam, and 200g / L chlorantraniliprole had a mortality rate of 100%. At 7 days after emergence, the mortality rates of the two treatments of 10% chlorantraniliprole + 30% clothianidin and 20% cyantraniliprole + 20% thiamethoxam were above 90%, the mortality rate of the treatment of 30% thiamethoxam and 200g / L chlorantraniliprole reached 86.18%, and the mortality rate of the treatment of 30% clothianidin and 10% cyantraniliprole was lower than 80%; 14-28 days after emergence, the mortality rates of 20% cyantraniliprole + 20% thiamethoxam and 10% chlorantraniliprole + 30% clothianidin were higher than those of the other four single-dose treatments, and among the single-dose treatments, the mortality rate of the treatment of 200g / L chlorantraniliprole and 30% thiamethoxam was significantly higher than that of the treatment of 30% clothianidin and 10% cyantraniliprole.

[0049] 1.2 Field evaluation of seed coating efficacy against adult beet beetles:

[0050] 7d-28d after emergence, the damage rates of each treatment were lower than those of the control. The damage rates of the compound seed dressing agents 10% chlorantraniliprole + 30% clothianidin and 20% cyantraniliprole + 20% thiamethoxam were lower. The damage rates of the single agent treatments 30% thiamethoxam and 200g / L chlorantraniliprole were lower.

[0051] Example 2

[0052] according to Figure 7-Figure 9 As shown: Safety evaluation of seed coating agent on quinoa plants:

[0053] The experiment was designed with 7 treatments: (1) chlorantraniliprole (CHL); (2) cyantraniliprole (CYA); (3) chlorantraniliprole·thiamethoxam (CHL+CLO); (4) bromothiocarb·thiamethoxam (CYA+THI); (5) clothianidin (CLO); (6) thiamethoxam (TH I); (7) control (CK) To further clarify the effect of seed coating agents on quinoa growth, 20 quinoa seedlings were randomly selected from each treatment 14 days after quinoa seedlings emerged, and their plant height, root length, and fresh weight of aboveground and underground parts were measured to evaluate the effects of the six seed coating agents on the agronomic traits of quinoa. After quinoa was harvested, 200g samples were selected from each treatment, and the seed coating agent residue detection was entrusted to Qingdao Huace Testing Technology Co., Ltd. for testing. On the 3rd, 7th, 14th, 21st and 28th days after quinoa seedlings emerged, quinoa seedlings from each treatment in each time period were selected to prepare crude enzyme solution and stored in a -20℃ refrigerator. The contents of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glucose-6-phosphate dehydrogenase (G-6-PDH), and total phenols (Total Pheno ls) were detected using corresponding kits, and the kits were all determined by visible light spectrophotometry.

[0054] Effect of seed coating agent on germination rate:

[0055] according to Figure 8 As shown in the figure, the emergence rate of quinoa in the field was higher than 90% in all treatments, and the difference was not significant. There was no significant difference in the emergence rate between the six treatments and the control.

[0056] according to Figure 8 Shown: Effects of seed coating treatment on plant height, root length, aboveground fresh weight and root weight of quinoa (Note: All data in the figure are mean ± standard deviation. Different letters on the column indicate treatments with P < 0.05 by the new multiple range test);

[0057] according to Fig. 9 Shown: Seed coating agent residues in quinoa seeds:

[0058] The residues of the six drugs and their metabolites in quinoa seeds were all less than 0.01 mg / kg.

[0059] Example 3

[0060] according to Fig.10 Shown: Determination of the indoor control effect of biological insecticides on beet beetles:

[0061] Screen the types of biological pesticides with good control effects on beet tube weevils. The 7 types of biological pesticides tested were 0.5% avermectin benzoate + 4000I U / mg Bacillus thuringiensis SC (Jiangxi Huihe Chemical Co., Ltd.), 10 billion spores / g Metarhizium anisopliae OD (Guangdong Xinxiang Bioengineering Co., Ltd.), 1 billion PI B / mL Beet Armyworm Nuclear Polyhedrosis Virus SC (Hunan Zefeng Agrochemical Co., Ltd.), 8000I U / μL Bacillus thuringiensis SC (Shandong Yijia Agrochemical Co., Ltd.), 10 billion spores / mg Bacillus brevis SC (Zhenjiang Runyu Biotechnology Development Co., Ltd.), 1.5% pyrethrin EW (Inner Mongolia Qingyuanbao Biotechnology Co., Ltd.) and 60g / L Ethyl spinosad SC (Corteva Agricultural Technology Co., Ltd.). The biological pesticides were purchased in May 2023. The tested biological pesticides were within the shelf life during indoor and outdoor tests. Each biological pesticide was prepared using a concentration gradient according to the instructions. For detailed information on the biological drugs, see Figure 1 .

[0062] The immersion method was used to determine the biological activity of the adults indoors. Test agents: Each agent was set with 5 mass concentration gradients, repeated 3 times, and clean water was used as a control. Quinoa leaves of similar size (after weighing) were immersed in the solution for 20 seconds, taken out and dried naturally, and a single leaf was moved into a 10 cm diameter culture dish. Each culture dish was filled with 10 adults after starvation treatment for 24 hours. The mortality of each age was observed 24 hours after the application of the drug. Gently touch the sensitive parts of the head, abdomen and feet of the beet tube beetle. If there is no reaction, it is considered as individual death. Record the number of deaths in each treatment, fit the regression equation, and determine the LC50 value.

[0063] The toxicity of beet tube beetle adults was determined by the immersion method. The results showed that spinetoram had the highest toxicity, with an LC50 value of 1248 mg / L 24 hours after application, followed by Metarhizium anisopliae, with an LC50 value of 2053 mg / L 24 hours after application, and pyrethrin had the lowest toxicity, with an LC50 value of 7115 mg / L 24 hours after application. The order of toxicity was: spinetoram>Metarhizium anisopliae>Bacillus brevis>Bacillus thuringiensis>Spodoptera exigua nuclear polyhedrosis virus>Bacillus thuringiensis alpha>pyrethrin. Figure 5 From the linear regression equation, we can see that the slope (b) and intercept of spinetoram are the highest and the largest 24h after treatment, indicating that the adults of beet beetle beetles are most sensitive to spinetoram and the drug has the highest starting point for the population reduction rate of beet beetle beetles. The starting point of the population reduction rate of pyrethrin is the lowest.

[0064] Example 4

[0065] according to Fig.11 Shown: Field control effect of biological insecticides on beet beetles

[0066] The experiment was conducted at the Science and Technology Innovation Base of Chengde Academy of Agriculture and Forestry Sciences. Quinoa was sown on May 27, 2023, with a sowing depth of 2.5 cm. All agricultural operations during the quinoa growing season were consistent. According to the results of the indoor biological activity test of the above 7 pesticides, the 4 biological pesticides screened indoors were used in the field at the quinoa seedling stage ( Figure 1 7-21) were sprayed. 10 billion spores / g Metarhizium anisopliae OD, 10 billion spores / mg Bacillus brevis SC, 60 g / L Spinetoram SC. Three dose gradients were set for each agent, and each dose was repeated 3 times. 10 billion spores / g of Metarhizium anisopliae OD: 92g / 667m2, 138g / 667m2, 248g / 667m2; 8000I U / μL of Bacillus thuringiensis SC: 110g / 667m2, 148g / 667m2, 226g / 667m2; 10 billion spores / mg of Bacillus brevis SC: 100g / 667m2, 158g / 667m2, 311g / 667m2; 60g / L of Ethyl Spinosad SC: 56g / 667m2, 80g / 667m2, 133g / 667m2; The second spraying treatment was carried out after 14 days. Each plot has an area of ​​50m2, with a 1m wide isolation zone between plots, and the plots are arranged in random blocks. Use a manual sprayer to spray the pesticide evenly, and the pesticide application rate in the plot is 2.25L. During the investigation, five diagonal points were sampled in each plot, and five quinoa plants were randomly selected at each point to observe the number of eggs laid on the plants on the day of application and the number of egg-laying holes on the plants 3d, 5d, and 7d after application. The control effect was judged based on the increase in the number of egg-laying holes.

[0067] The seven biological pesticides had a certain control effect on the number of eggs laid by the beet tube weevil, and the four pesticides had a certain control effect on the beet tube weevil. Because it was impossible to directly observe the death of the adult insects, the relative control effect was calculated based on the number of eggs laid. The control effect of the ethyl spinetoram treatment group was the best, at 67.39%, followed by pyraclostrobin (45.66%), difenoconazole (45.66%), polyoxin (41.31%) and pyrimethanil (41.31%). From the perspective of control effect and persistence, 60g / L ethyl spinetoram SC and 10 billion spores / g Metarhizium anisopliae OD had a good control effect on the beet tube weevil.

[0068] Example 5

[0069] according to Fig.12 Shown: The control effect of seed coating agent and biological insecticide on beet beetle weevil during the whole growth period of quinoa

[0070] The seed coating agent was 10% chlorantraniliprole + 30% clothianidin (1200ml / 100kg); the dosage of 20% cyantraniliprole + 20% thiamethoxam was (450mL / 100kg), and the dosage of 200g / L chlorantraniliprole was (900mL / 100kg). The biological insecticides were 10 billion spores / g Metarhizium anisopliae OD, 10 billion spores / mg Bacillus brevis SC and 60g / L spinetoram SC. The seed coating agent and the insecticide were used in pairs to carry out the control test of beet tube weevils by seed coating + spraying. Treatment 1: 10% chlorantraniliprole + 30% clothianidin 1200ml / 100kg + 10 billion spores / g scarab green anisopliae OD: 2.053g / L; Treatment 2: 20% cyantraniliprole + 20% thiamethoxam 450mL / 100kg + 10 billion spores / g scarab green anisopliae OD 2.053g / L; Treatment 3: 200g / L chlorantraniliprole 900mL / 100kg + 10 billion spores / g scarab green anisopliae OD 2.053g / L; Treatment 4: 30% thiamethoxam 400ml / 100kg + 10 billion spores / g scarab green anisopliae OD 2.053g / L; Treatment 5: 10% chlorantraniliprole + 30% clothianidin 1200ml / 100kg + 10 billion spores / g scarab green anisopliae OD 3.08g / L; Treatment 6: 20% cyantraniliprole + 20% thiamethoxam 450mL / 100kg + 10 billion spores / g scarab green anisopliae OD 3.08g / L; Treatment 7: 200g / L chlorantraniliprole 900mL / 100kg + 10 billion spores / g scarab green anisopliae OD 3.08g / L; Treatment 8: 30% thiamethoxam 400m l / 100kg + 10 billion spores / g scarab green anisopliae OD 3.08g / L; Treatment 9: 10% chlorantraniliprole + 30% clothianidin 1200m l / 100kg + 10 billion spores / mg short stable bacillus SC 6.91g / L; Treatment 10: 20% cyantraniliprole + 20% thiamethoxam 450mL / 100kg + 10 billion spores / mg short stable bacillus SC 6.91g / L; Treatment 11: 200g / L chlorantraniliprole 900mL / 100kg+10 billion spores / mg short stable bacillus SC 6.91g / L; Treatment 12: 30% thiamethoxam 400ml / 100kg+10 billion spores / mg short stable bacillus SC 6.91g / L; Treatment 13: 10% chlorantraniliprole + 30% clothianidin 1200ml / 100kg+60g / L ethyl spinetoram SC 1.24g / L; Treatment 14: 20% cyantraniliprole + 20% thiamethoxam 450mL / 100kg+60g / L ethyl spinetoram SC 1.24g / L; Treatment 15: 200g / L chlorantraniliprole 900mL / 100kg+60g / L ethyl spinetoram SC1.24g / L; Treatment 16: 30% thiamethoxam 400ml / 100kg + 60g / L ethyl spinetoram SCSC 1.24g / L; Treatment 17: 10% chlorantraniliprole + 30% clothianidin 1200ml / 100kg + 60g / L ethyl spinetoram SC 1.78g / L; Treatment 18: 20% cyantraniliprole + 20% thiamethoxam 450mL / 100kg + 60g / L ethyl spinetoram SC 1.78g / L; Treatment 19: 200g / L chlorantraniliprole 900mL / 100kg + 60g / L ethyl spinetoram SC 1.78g / L; Treatment 20: 30% thiamethoxam 400ml / 100kg + 60g / L ethyl spinetoram SC 1.78g / L; Treatment 21: CK. .

[0071] Seeds coated with 10% chlorantraniliprole + 30% clothianidin, 20% cyantraniliprole + 20% thiamethoxam, and 200g / L chlorantraniliprole were sown respectively, and the first spraying treatment was carried out after planting the seedlings, the second spraying treatment was carried out after an interval of 14 days, and the third spraying treatment was carried out during the grain filling period in early August. The uncoated spraying plot was used as the control. The control effect of different treatment groups was calculated.

[0072] Depend on Fig.12 It can be seen that the control effects of the combination of compound seed coating agent + green muscardine and compound seed coating agent + ethyl spinetoram in the seedling stage are all above 70%; the control effects in the flowering stage are all above 80%; and the control effects in the filling stage are above 85%. The yield of 20% cyantraniliprole + 20% thiamethoxam 450mL / 100kg + 10 billion spores / g green muscardine OD 3.08g / L and 10% chlorantraniliprole + 30% clothianidin 1200ml / 100kg + 60g / L ethyl spinetoram SC 1.24g / L treatments are both above 180kg / 667m2, and the yield of the control group is 9.05kg / 667m2.

[0073] The specific implementation manner of the present invention is further described in detail below in conjunction with Examples 1-5.

[0074] Test species and insect sources:

[0075] The test variety is Yanli No. 1, provided by the Cereal Crops Research Group of Chengde Academy of Agricultural and Forestry Sciences. It was sown on May 27, 2023, and field seed dressing agent screening tests and biological pesticide field screening tests were carried out. It was sown on May 6, 2024, and a combination screening test of seed dressing agents and biological pesticides was carried out. The adult beet tube beetles were collected from Zhongguan Town, Longhua County, Chengde City (22°43′46″N, 100°53′59″E), placed in an insect tank, and fed with fresh quinoa leaves at room temperature.

[0076] Seed coating agent and spraying biological pesticide for testing:

[0077] The information and dosage of the 6 tested seed coating agents (No. 1-6) and 6 spray insecticides (No. 7-12) are shown in Figure 1 When using the pesticide, it should be diluted with water in proportion and then sprayed.

[0078] Test equipment and kits

[0079] Air pressure sprayer, insect tank (9 cm in diameter, 15 cm in height), insect tweezers, brush, test tube, beaker, 100 mL measuring cylinder, glass rod, pipette and gun tip, culture dish, analysis filter paper, plastic gloves, cotton wool, marker, FA2104B electronic balance (Shanghai Precision Instrument Co., Ltd.).

[0080] The embodiments of the present application are described above in conjunction with the accompanying drawings. In the absence of conflict, the embodiments in the present application and the features in the embodiments can be combined with each other. The present application is not limited to the above-mentioned specific implementation methods. The above-mentioned specific implementation methods are merely illustrative and not restrictive. Under the guidance of the present application, ordinary technicians in this field can also make many forms without departing from the purpose of the present application and the scope of protection of the claims, all of which are within the protection of the present application.

Claims

1. A method for controlling quinoa stem borer pest beetle beetle, characterized in that: The specific steps include: Step S1: Seed coating: After rinsing with sterile water for 3 times, place the quinoa seeds on sterile filter paper, dry at room temperature, and set aside; Take a seed coating agent, add an appropriate amount of sterile water to the seed coating agent and mix well to make a slurry, and add the dried seeds to coat them; Step S2: Spraying with biological insecticides: Spraying with biological insecticides: 20 days after quinoa seedlings emerge, when the seedling height is 12 cm to 20 cm, use 10 billion spores / g of Metarhizium anisopliae OD or 60 g / L spinetoram SC for the third spraying, and conduct the second spraying after an interval of 14 days to achieve the prevention and control of beet beetles throughout the growth period of quinoa; During the grain filling period of quinoa, a third spray was performed using 10 billion spores / g of Metarhizium anisopliae OD or 60 g / L of ethyl spinosad SC.

2. A method for controlling the quinoa stem borer pest beetle beetle according to claim 1, characterized in that: The screening method of the seed coating agent comprises: Investigation of agronomic traits of quinoa seedlings: The pot method was used to determine the effects of seed coating agents on quinoa growth. 20 quinoa seedlings were randomly selected from each treatment plot 14 days after quinoa seedlings emerged. The plant height, root length, and fresh weight of above-ground and underground parts of quinoa were measured to evaluate the effects of the six agents on the agronomic traits of quinoa. Indoor evaluation of the efficacy of seed dressing agents against Beet Weevil: On the 3rd, 7th, 14th, 21st and 28th days after quinoa emergence, the test seedlings were collected and the plants were moistened with moist cotton balls. Ten quinoa plants were placed in each insect pot, which was then placed in an insect pot with 10 adult Beet Weevil. The test insect sources were starved for 2 hours before the test treatment and then transferred to the experimental plants. The death of the adults was observed after 3 days, and the adult mortality rate was investigated.

3. A method for controlling the quinoa stem borer pest beetle beetle according to claim 1, characterized in that: The method for screening the seed coating agent also includes: Field experiment. The specific method of the field experiment is as follows: the experiment is designed with 7 treatments: (1) chlorantraniliprole; (2) cyantraniliprole; (3) chlorantraniliprole·thiamethoxam; (4) bromothiocarb·thiamethoxam; (5) clothianidin; (6) thiamethoxam; (7) control. No treatment is given. Each treatment is repeated 4 times, with a total of 28 plots. The plots are arranged in random blocks. On the 7th, 14th, 21st and 28th days after emergence, the quinoa plants damaged by the beet beetle in each treatment plot are investigated, and the damage rate is calculated. Finally, the seed dressing agent is selected as follows: 10% chlorantraniliprole + 30% clothianidin or 20% cyantraniliprole + 20% thiamethoxam or 200g / L chlorantraniliprole.

4. A method for controlling the quinoa stem borer pest, the beet beetle weevil, according to claim 1, characterized in that: The biopesticide spray screening method comprises: Through indoor toxicity determination, the number of deaths in each treatment is recorded and the relative toxicity index is calculated to screen biopesticides; Field efficacy plot tests are used to verify the results of the pesticides screened out by indoor toxicity tests.

5. A method for controlling the quinoa stem borer pest, the beet beetle weevil, according to claim 4, characterized in that: The specific method for screening biological insecticides by the indoor toxicity determination is as follows: the indoor biological activity determination of the beet tube beetle adult is carried out by the leaf immersion method, which is repeated 3 times, and clean water is used as a control. The quinoa leaves of similar size are immersed in the liquid for 20 seconds, taken out and dried naturally, and the leaves are placed in a culture dish with a diameter of 10 cm, and 10 adults after starvation treatment for 24 hours are placed in each culture dish. The death situation is observed 24 hours and 48 hours after the application of the pesticides. According to the results of the indoor biological activity determination of the insecticides, 10 billion spores / g of Metarhizium anisopliae OD, 8000IU / μL Bacillus thuringiensis SC, 10 billion spores / mg Bacillus brevis SC, and 60g / L ethyl spinetoram SC are selected for the field test. Three dosage gradients are set for each agent, and each dosage is repeated 3 times. The number of eggs laid on the plant on the day of application and the number of egg-laying holes on the plant 3 days, 5 days, 7 days and 15 days after application are observed, and the prevention effect is judged according to the increase in the number of egg-laying holes. Finally, the biological insecticides with better control effects were obtained: 60g / L spinetoram SC and 10 billion spores / g Metarhizium anisopliae OD.

6. A method for controlling the quinoa stem borer pest, the beet beetle weevil, according to claim 1, characterized in that: The quinoa filling period is early August.

7. A method for controlling the quinoa stem borer pest, the beet beetle weevil, according to claim 3, characterized in that: The dosage of the 10% chlorantraniliprole + 30% clothianidin is 1200ml / 100kg seeds; The dosage of 20% cyantraniliprole + 20% thiamethoxam is 450mL / 100kg seeds, and the dosage of 200g / L chlorantraniliprole is 900mL / 100kg seeds.

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