Application of camphene in promoting seed germination and seedling growth of Panax notoginseng

Abstract

The invention relates to the technical field of Panax notoginseng planting, and in particular to the application of camphene in promoting the germination of Panax notoginseng seeds and the growth of seedlings. The invention can increase the seed germination rate of Panax notoginseng by fumigating the soil for planting Panax notoginseng with camphene, a volatile substance, and then planting Panax notoginseng seeds on the soil fumigated with camphene, thereby helping the Panax notoginseng seedlings to increase biomass, increasing the above-ground development of Panax notoginseng, promoting the extension of the roots of Panax notoginseng, facilitating the absorption of soil nutrients by Panax notoginseng, and having a significant promoting effect on the germination of Panax notoginseng seeds and the growth of seedlings.

Description

Technical Field

[0001] The invention relates to the technical field of notoginseng planting, and in particular to the application of camphene in promoting notoginseng seed germination and seedling growth. Background Art

[0002] Panax notoginseng is a perennial herbaceous plant of the genus Panax, family Araliaceae, order Apiaceae. It is also known as ginseng, field notoginseng, field notoginseng, mountain lacquer, panlongqi, jinbuhuan and copper skin and iron bones. It is recorded in ancient medical books such as "Compendium of Materia Medica", "Bencao Qiuzhen", "Bencao Congxin" and "Compendium of Materia Medica Supplement". It has the characteristics of warm nature, sweet and slightly bitter taste, and has good effects such as removing blood stasis and stopping bleeding, promoting blood circulation and relieving pain. It is a good medicine widely recognized by the medical community. It is mainly used as medicine in the form of dried tubers and flowers, and there are various ways to process and eat it. Due to its high medicinal value, wild picking resources can no longer meet the growing social demand, so modern artificial farmland cultivation is widely adopted.

[0003] Up to now, the development of the Panax notoginseng planting industry has made great progress, but there is a big gap compared with traditional crop planting, especially the growth problem of Panax notoginseng caused by continuous cropping obstacles has seriously restricted the sustainable development of the Panax notoginseng industry. In production practice, the occurrence of continuous cropping obstacles usually leads to problems such as reduced germination rate of Panax notoginseng seeds and limited growth of Panax notoginseng seedlings, resulting in reduced Panax notoginseng production and affecting planting benefits. It also causes a series of problems that touch the ecological red line, such as deforestation, occupation of arable land and excessive agricultural residues. In order to further improve the problem of continuous cropping obstacles, a variety of solutions have been proposed in the prior art, such as improving the physical and chemical properties of the soil according to the growth needs of Panax notoginseng, such as adjusting the soil pH and increasing the organic matter content; or implementing a reasonable rotation system to avoid long-term continuous cropping of the same crop or crops of the same family; or formulating a scientific fertilization plan by measuring the nutrient content of the soil. However, the above solutions all have certain limitations and cannot significantly improve the germination and growth rate of Panax notoginseng seeds. Therefore, how to improve the germination of Panax notoginseng seeds and promote the growth of Panax notoginseng plants in a simple and feasible way in daily planting is not only a production problem that needs to be solved urgently, but also an ecological problem of sustainable development. Summary of the invention

[0004] In view of the shortcomings of the prior art, the present invention places camphene, a volatile substance, on the continuously cropped soil for fumigation, which can subsequently promote the germination of Panax notoginseng seeds and the growth of seedlings.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0006] The invention proposes the application of camphene in promoting the germination of notoginseng seeds and the growth of seedlings.

[0007] Furthermore, the application method is to use camphene to fumigate the Panax notoginseng planting soil in a closed space, and then plant the Panax notoginseng seeds on the fumigated Panax notoginseng planting soil; the Panax notoginseng planting soil includes continuous cropping soil or normal planting soil.

[0008] Further, the concentration of camphene in the confined space is 0.1-50 g / L, for example, 0.1 g / L, 1 g / L, 2 g / L, 5 g / L, 8 g / L, 15 g / L, 20 g / L, 25 g / L, 30 g / L, 50 g / L.

[0009] Preferably, the concentration of camphene in the confined space is 5 g / L.

[0010] Furthermore, the fumigation time is 1-3 days, for example, 1 day, 2 days, or 3 days.

[0011] Preferably, the fumigation time is preferably 3 days.

[0012] Compared with the prior art, the present invention has the following advantages:

[0013] The present invention can increase the seed germination rate of Panax notoginseng by pre-fumigating the Panax notoginseng planting soil with camphene, a volatile substance, and then planting Panax notoginseng seeds on the continuously planted soil after the camphene fumigation, help Panax notoginseng seedlings to increase biomass, increase the above-ground development of Panax notoginseng, promote the extension of the roots of Panax notoginseng, facilitate the absorption of soil nutrients by Panax notoginseng, and have a significant promoting effect on the germination of Panax notoginseng seeds and the growth of seedlings. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0015] Figure 1 The germination effect diagram of Panax notoginseng seeds after the continuous cropping soil of Panax notoginseng is treated by fumigation induction with camphene of different concentrations in the embodiment of the present invention is shown;

[0016] Figure 2 The germination rate result diagram of Panax notoginseng seeds after the continuous cropping soil of Panax notoginseng is treated by fumigation induction with camphene of different concentrations in the embodiment of the present invention is shown;

[0017] Figure 3 The results of the seedling survival rate of Panax notoginseng seeds after the continuous cropping soil of Panax notoginseng is treated by fumigation induction with camphene at different concentrations in the embodiment of the present invention are shown;

[0018] Figure 4The fresh weight result graph of Panax notoginseng seedlings after the soil of Panax notoginseng continuous cropping was treated by fumigation induction with camphene at different concentrations in the embodiment of the present invention is shown;

[0019] Figure 5 The result diagram of the stem length of Panax notoginseng seedlings after the soil of Panax notoginseng continuous cropping was treated by fumigation induction with camphene of different concentrations in the embodiment of the present invention is shown;

[0020] Figure 6 A comparative scan of the roots of Panax notoginseng seedlings after the soil of Panax notoginseng continuous cropping was treated by fumigation induction with camphene at different concentrations in an embodiment of the present invention is shown;

[0021] Figure 7 The result diagram of the root length of Panax notoginseng seedlings after the continuous cropping soil of Panax notoginseng is treated by fumigation induction with camphene of different concentrations in the embodiment of the present invention is shown;

[0022] Figure 8 The result graph of the root area of ​​Panax notoginseng seedlings after the soil of Panax notoginseng continuous cropping was treated by fumigation induction with camphene at different concentrations in an embodiment of the present invention is shown. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention is clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Example 1

[0024] Experiment on the induction of Panax notoginseng seeds by camphene fumigation:

[0025] The notoginseng seeds used in the present embodiment are purchased from the notoginseng trading market in Wenshan City, Yunnan Province, the notoginseng authentic production area, and the notoginseng continuous cropping soil used is obtained from the Daheqiao experimental base in Xundian. The notoginseng seeds are rinsed with sterile water for 5 minutes for standby use after the sodium hypochlorite immersion disinfection that is 3% by mass fraction. The camphene standard product is selected from Merck Sigma-Aldrich company, and its purity is greater than or equal to 97%. The airtight box specification for the experiment is 50cm long, 35cm wide, and 30cm high.

[0026] Camphene standards were weighed at 0 g / L, 0.1 g / L, 1 g / L, 5 g / L, 20 g / L, and 50 g / L according to the volume of a sealed box and placed in a bottle cap (28 mm in diameter), which was then placed on 1 kg of continuously cropped soil for 72 h of sealed fumigation.

[0027] After the fumigation treatment, the continuous cropping soil was taken for the Panax notoginseng seed germination and seedling growth test. Each 50g continuous cropping soil was weighed and put into one tissue culture bottle. Each concentration treatment was repeated with 20 bottles, totaling 120 bottles of soil. Subsequently, 12 previously sterilized Panax notoginseng seeds were evenly dotted in each bottle of soil along a ring with tweezers, and then 20mL of sterile water was poured in using a bottle mouth dispenser. After the bottle cap was tightly closed, it was moved into a constant temperature greenhouse at 25℃ for cultivation, with a light duration of 12 hours per day, waiting for the Panax notoginseng seeds to emerge. Example 2

[0028] The growth of the Panax notoginseng seedlings cultivated in Example 1 was confirmed, and after the Panax notoginseng seeds emerged completely in 60 days, the germination rate of the Panax notoginseng seeds planted on the soil fumigated with different concentrations of camphene was counted, germination rate=number of seedlings / number of seeds × 100%.

[0029] After 120 days of stable growth of Panax notoginseng seedlings, the survival rate of Panax notoginseng seedlings planted in soil fumigated with different concentrations of camphene was counted, and the survival rate = number of surviving seedlings / number of seeds X 100%. Then take out the Panax notoginseng seedlings from the tissue culture bottle, clean them, weigh the fresh weight of the Panax notoginseng seedlings with a balance, and measure the stem length of the Panax notoginseng seedlings with a ruler. The root length and root area of ​​the Panax notoginseng seedlings were measured using an EpsonPerfection V850 Pro scanner. IBM SPSS 19 was used to calculate the difference of the relevant data, and finally Graphpad prism9 was used for drawing and presentation. The results are as follows:

[0030] Germination effect:

[0031] The germination effect of Panax notoginseng seeds in each concentration experimental group is as follows Figure 1 As shown, it can be seen that the germination and growth effects of Panax notoginseng seeds were better after fumigating the continuous cropping soil with five concentrations of camphene (0.1g / L, 1g / L, 5g / L, 20g / L, 50g / L). Compared with the unfumigated treatment (0g / L), fumigating the continuous cropping soil with five concentrations of camphene (0.1g / L, 1g / L, 5g / L, 20g / L, 50g / L) and then planting Panax notoginseng seeds will make the Panax notoginseng seedlings have taller stems, larger leaves, and denser root systems.

[0032] The germination rate of Panax notoginseng seeds in each concentration experimental group is as follows: Figure 2 As shown, it can be seen that after fumigation treatment of continuous cropping soil with five camphene concentrations (0.1g / L, 1g / L, 5g / L, 20g / L, 50g / L), the germination rate of Panax notoginseng seeds was improved. After the 1g / L camphene fumigation treatment, the germination rate was significantly improved compared with the non-fumigation treatment (0g / L), among which the germination rate of Panax notoginseng seeds after 5g / L camphene fumigation continuous cropping soil was the highest, reaching 52%. This shows that planting Panax notoginseng after camphene fumigation continuous cropping soil has the effect of promoting the germination of Panax notoginseng seeds.

[0033] Seedling survival rate:

[0034] The survival rate of Panax notoginseng seedlings in each concentration experimental group is as follows: Figure 3 As shown in the figure, it can be seen that after fumigating the continuous cropping soil with five camphene concentrations (0.1g / L, 1g / L, 5g / L, 20g / L, and 50g / L), the survival rate of Panax notoginseng seedlings was significantly improved. Among them, the survival rate of Panax notoginseng seedlings after fumigating the continuous cropping soil with 5g / L camphene was the highest, reaching 37.9%. This shows that planting Panax notoginseng after fumigating the continuous cropping soil with camphene has the effect of stabilizing the growth of Panax notoginseng seedlings and reducing the number of Panax notoginseng seedling deaths.

[0035] Fresh weight:

[0036] The average fresh weight of Panax notoginseng seedlings in each concentration experimental group is as follows Figure 4 As shown in the figure, after fumigation of continuous cropping soil with five camphene concentrations (0.1g / L, 1g / L, 5g / L, 20g / L, 50g / L), the fresh weight of Panax notoginseng seedlings was increased. Among them, the fresh weight of Panax notoginseng seedlings per plant was the highest after continuous cropping soil fumigation with 5g / L camphene, exceeding 0.36g. This shows that planting Panax notoginseng after continuous cropping soil fumigation with camphene has the effect of promoting the growth of Panax notoginseng seedlings and increasing the biomass of Panax notoginseng.

[0037] Stem length:

[0038] The average stem length of Panax notoginseng seedlings in each concentration experimental group is as follows Figure 5 As shown in the figure, it can be seen that after fumigation of continuous cropping soil with five camphene concentrations (0.1g / L, 1g / L, 5g / L, 20g / L, 50g / L), the stem length of Panax notoginseng seedlings was improved. Among them, the stem length of Panax notoginseng seedlings after continuous cropping soil fumigation with 20g / L camphene was the highest, reaching an average of 7cm. This shows that planting Panax notoginseng after continuous cropping soil fumigation with camphene promoted the development of the aboveground part of Panax notoginseng seedlings and increased the length of the stem.

[0039] Root:

[0040] The root scanning results of Panax notoginseng seedlings in each concentration experimental group are as follows: Figure 6 As shown, it can be seen that the root system of Panax notoginseng seedlings developed better after fumigating the continuous cropping soil with five concentrations of camphene (0.1g / L, 1g / L, 5g / L, 20g / L, 50g / L). Compared with the unfumigated treatment (0g / L), fumigating the continuous cropping soil with five concentrations of camphene (0.1g / L, 1g / L, 5g / L, 20g / L, 50g / L) and then planting Panax notoginseng seeds will lead to a well-developed root system, dense root system, and longer root hairs.

[0041] The statistical results of root length are as follows: Figure 7As shown in the figure, it can be seen that after fumigation of continuous cropping soil with five camphene concentrations (0.1g / L, 1g / L, 5g / L, 20g / L, 50g / L), the root length of Panax notoginseng seedlings was significantly increased, among which the average root length of Panax notoginseng seedlings after continuous cropping soil fumigation with 1g / L camphene was the highest, exceeding 21cm. This shows that planting Panax notoginseng after continuous cropping soil fumigation with camphene can promote the extension of Panax notoginseng roots and increase root length.

[0042] The statistical results of the root area of ​​a single plant are as follows: Figure 8 As shown in the figure, it can be seen that after the continuous cropping soil was fumigated with five camphene concentrations (0.1g / L, 1g / L, 5g / L, 20g / L, and 50g / L), the root area of ​​Panax notoginseng seedlings was increased. Among them, the root area of ​​Panax notoginseng seedlings after continuous cropping soil was fumigated with 5g / L camphene was the largest, reaching 0.41cm 2 This shows that planting Panax notoginseng after fumigating the soil with camphene can help the development of Panax notoginseng fibrous roots, increase the coverage area of ​​fibrous roots, and facilitate better absorption of soil nutrients.

[0043] It should be noted that the germination rate of Panax notoginseng seeds in the embodiment of the present invention ( Figure 2 ), the survival rate of Panax notoginseng seedlings ( Figure 3 )、Fresh weight( Figure 4 )、Stem length( Figure 5 ), root length ( Figure 7 ), root area ( Figure 8 ) are expressed as mean ± standard error (SEM), and different lowercase letters represent significant differences between treatments (P<0.05, one-way analysis of variance, Duncan's multiple range test). Among them, a, b, and c represent different treatment groups with significant differences. ab means that there is no statistically significant difference between treatment group a and treatment group b, but they are significantly different from the treatment group marked as c.

[0044] As can be seen from the above-described embodiments, after continuous cropping soil fumigation with camphene, planting Panax notoginseng can increase the seed germination rate of Panax notoginseng, help Panax notoginseng seedlings to improve biomass, increase the above-ground development of Panax notoginseng, promote the root extension of Panax notoginseng, and facilitate the absorption of soil nutrients by Panax notoginseng. Among them, the camphene fumigation concentration of 5g / L is the most preferred and most suitable.

[0045] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. The application of camphene in promoting the germination of Panax notoginseng seeds and the growth of seedlings is characterized in that: The application method is to use camphene to fumigate the Panax notoginseng planting soil in a closed space, and then plant the Panax notoginseng seeds on the fumigated Panax notoginseng planting soil. The concentration of camphene in the closed space is 0.1-50g / L.

2. The use according to claim 1, characterized in that: The concentration of camphene in the confined space is 5g / L.

3. The use according to claim 1, characterized in that: The fumigation time is 1-3 days.

4. The use according to claim 1 or 3, characterized in that: The fumigation time is 3 days.

Images