Natural anti-dandruff agent and application thereof

Abstract

The invention relates to the field of daily chemicals, in particular to a natural anti-dandruff agent and application thereof. The natural anti-dandruff agent is prepared from the following components in parts by weight: 0.01 to 5 parts of carvacrol, 0.01 to 10 parts of radix astragali seu hedysari extract and 0.01 to 10 parts of hamamelis virginiana extract. The natural anti-dandruff agent provided by the invention contains the carvacrol, the alpine astragalus extract and the hamamelis virginiana extract, and the three natural components have a synergistic effect, so that the composition has mild, antibacterial and anti-inflammatory effects, not only can effectively remove dandruff, but also can promote scalp health and recover natural balance.

Description

Technical Field

[0001] The invention relates to the technical field of shampoo, and in particular to a natural anti-dandruff agent and application thereof. Background Art

[0002] The generation of dandruff is a complex process involving multiple factors. Generally speaking, dandruff is mainly caused by the excessive proliferation of fungi (such as Malassezia) on the scalp. These fungi feed on sebum on the scalp, decomposing sebum to produce free fatty acids and other metabolites. These substances stimulate the scalp to produce an inflammatory response, leading to scalp itching, sensitivity and other problems, and further stimulate the imbalance of sebaceous gland secretion, aggravating symptoms such as dandruff.

[0003] In the hair cosmetics market, anti-dandruff products account for 35%. Among them, the most commonly used anti-dandruff ingredient is ZPT. ZPT is the abbreviation of zinc pyrithione, which can effectively inhibit the growth of Malassezia, which causes dandruff. ZPT mainly works by inhibiting the growth and reproduction of microorganisms. It can interfere with the metabolic process of microbial cells. Specifically, ZPT acts on the cell membrane and cell wall of microorganisms, destroying their integrity, causing leakage of intracellular substances, and then inhibiting the growth and reproduction of microorganisms. However, many people may experience allergic reactions such as scalp itching and skin redness and swelling when using products containing ZPT. Therefore, it is necessary to find an anti-dandruff agent that is comparable to or even better than ZPT in anti-dandruff ability but is safer and less irritating. Summary of the invention

[0004] In view of this, the present invention provides a natural anti-dandruff agent and application thereof, aiming to solve the defect of high irritation of existing anti-dandruff agents.

[0005] As a first aspect of the present invention, the present invention provides a natural anti-dandruff agent, which comprises, by weight, 0.01 to 5 parts of carvacrol, 0.01 to 10 parts of alpine astragalus extract, and 0.01 to 10 parts of North American witch hazel extract.

[0006] Carvacrol can inhibit the growth of bacteria on the scalp and reduce scalp inflammation, thereby reducing the occurrence of dandruff. It can also regulate the oil secretion of the scalp, keep the scalp fresh, help improve the scalp environment and reduce dandruff. Alpine Astragalus extract can enhance the immunity of the scalp and improve the scalp's resistance to external stimuli, thereby reducing the occurrence of dandruff. It can also promote blood circulation in the scalp, provide adequate nutrition to the scalp, and help improve scalp health. North American witch hazel extract can tighten the scalp pores and reduce the oil secretion of the scalp, thereby reducing the occurrence of dandruff. North American witch hazel extract can also soothe scalp discomfort, reduce scalp itching and inflammation, and help improve the scalp environment.

[0007] A natural anti-dandruff agent containing carvacrol, alpine astragalus extract and North American witch hazel extract. This combination has multiple functions such as antibacterial and anti-inflammatory, nourishing and repairing, and oil control. It is gentle and safe and suitable for long-term use. The synergistic effect of the three natural forces not only effectively removes dandruff, but also promotes scalp health and restores natural balance.

[0008] In a preferred embodiment of the first aspect of the present invention, the natural anti-dandruff agent comprises, by weight: 0.1-0.2 parts of carvacrol, 0.2-5 parts of alpine astragalus extract, and 0.2-5 parts of witch hazel extract.

[0009] In a preferred embodiment of the first aspect of the present invention, the natural anti-dandruff agent comprises, by weight, 0.1-0.2 parts of carvacrol, 1-5 parts of alpine astragalus extract, and 0.2-5 parts of witch hazel extract.

[0010] In a preferred embodiment of the first aspect of the present invention, the natural anti-dandruff agent comprises, by weight, 0.1-0.2 parts of carvacrol, 1-5 parts of Astragalus membranaceus extract, and 1-5 parts of Hamamelis virginiana extract.

[0011] In a preferred embodiment of the first aspect of the present invention, the method for preparing the natural anti-dandruff agent comprises the following steps:

[0012] S1, adding Astragalus membranaceus extract and carvacrol to Hamamelis virginiana extract, using ultrasound-assisted extraction;

[0013] S2. After the extraction is completed, the solid residue is removed by centrifugation to obtain a liquid extract containing active ingredients;

[0014] S3, filtering and purifying the liquid extract by membrane separation;

[0015] S4. The purified extract is concentrated by low-temperature vacuum concentration, and then the concentrate is converted into a powdered product by spray drying or freeze drying technology.

[0016] Specifically, the natural anti-dandruff agent is prepared by the above method, wherein in step S1, ultrasound-assisted extraction technology is used to destroy the cell wall by using the cavitation effect of ultrasound, thereby increasing the dissolution rate of the active ingredient and improving the extraction efficiency and effect. The ultrasound power is 300-350W, the ultrasound time is 20-60min, and the ultrasound temperature is 40-60°C.

[0017] In step S2, after the ultrasonic extraction is completed, the solid residue is removed by centrifugal separation technology to obtain a liquid extract containing active ingredients. In this step, the centrifugal speed is 8000-12000rpm and the centrifugal time is 2-5min. Centrifugal separation can quickly and effectively separate insoluble solid impurities from the liquid extract to ensure the purity and quality of the extract.

[0018] In step S3, the extract is further purified using membrane separation technology, which can finely filter the extract according to characteristics such as molecular size, further remove impurities, thereby retaining the active ingredients with anti-dandruff effects and improving the purity and stability of the composition. Specifically, in this step, the filtration membrane used can be a combination of PS-10 ultrafiltration membrane and HPS-3 ultrafiltration membrane.

[0019] In step S4, the purified extract is concentrated by low-temperature vacuum to reduce the water content and increase the concentration of active ingredients, thereby avoiding the loss of heat-sensitive ingredients. Finally, the concentrate is converted into a powdered product that is easy to store by spray drying or freeze drying technology. Spray drying or freeze drying technology can make the concentrate into a powdered product that is easy to store, transport, and add to hair products.

[0020] In this step, low-temperature vacuum concentration is adopted, wherein the rotary evaporation temperature is 45-60°C, the time is 20min-40min, and the rotation speed is 20-40rpm.

[0021] In a preferred embodiment of the first aspect of the present invention, the natural anti-dandruff agent, the carvacrol is obtained by processing the caraway seeds to obtain carvacrol powder, and the carvacrol powder is subjected to supercritical carbon dioxide extraction to obtain carvacrol, wherein the extraction conditions are heating to 30-50°C and pressurizing to above 7.50MPa.

[0022] Specifically, the carvacrol is processed from the caraway seeds to obtain the carvacrol powder by chopping, grinding, sieving and drying the caraway seeds, wherein a 50-70 mesh screen is used for sieving, and the drying method is to turn over at 45-55° C. overnight to ensure complete drying, thereby obtaining the carvacrol powder.

[0023] Wherein, carvacrol powder is extracted with supercritical carbon dioxide to obtain carvacrol. The supercritical carbon dioxide extraction comprises the following steps:

[0024] S11: pressurizing the carbon dioxide to above 7.50 MPa to be in a supercritical state, and heating it to 30-50° C. to make it penetrate the plant material;

[0025] S21: Supercritical CO2 interacts with carvacrol and other compounds in carvacrol powder for 30 to 70 minutes to dissolve these components;

[0026] S31: The pressure is reduced to 0.1 MPa to restore the supercritical CO2 to a gaseous state, and carvacrol and other components are released and collected in a separator.

[0027] Supercritical carbon dioxide extraction has the advantages of high selectivity and no solvent residue. It can efficiently extract high-purity carvacrol from parsley seeds, ensuring its quality and activity.

[0028] In a preferred embodiment of the first aspect of the present invention, the pretreatment step of the high mountain astragalus extract includes washing, drying, crushing and sieving the high mountain astragalus, and then using supercritical carbon dioxide extraction to obtain the high mountain astragalus extract, the extraction temperature is 30-55°C, the extraction pressure is 30-50MPa, and the entrainer is 95% ethanol.

[0029] Specifically, the alpine astragalus is washed, dried, crushed and sieved to obtain the alpine astragalus powder; wherein the drying temperature is 40-50° C., and a 50-70 mesh screen is used for sieving;

[0030] Adjust the equipment to a pressure of 30-50 MPa and a temperature of 30-55°C;

[0031] The pretreated alpine astragalus powder was placed in an extractor and supercritical CO2 was added as an extractant; during the extraction process, the CO2 flow rate was adjusted to ensure adequate dissolution and mass transfer; at the same time, an entrainer could be added to improve the extraction efficiency and selectivity; the CO2 flow rate was adjusted to 3.7 L / H, and the entrainer was 95% ethanol;

[0032] When the CO2 fluid saturated with dissolved matter enters the separator, due to the pressure drop or temperature change, the CO2 and the extract quickly become two phases (gas-liquid separation) and are immediately separated; at this time, the extracted effective ingredients of the high mountain astragalus extract can be collected, and the pressure is reduced to 0.1MPa in this step.

[0033] Through such pretreatment and extraction process, the effective components in Alpine Astragalus can be fully extracted, and the quality and activity of the extract can be guaranteed by appropriate parameter control.

[0034] In a preferred embodiment of the first aspect of the present invention, the pretreatment step of the witch hazel extract comprises:

[0035] The North American witch hazel was crushed and sieved, and after adding boric acid, it was extracted at 60-70°C for 1-2 hours, and the supernatant was taken after filtering, and citric acid was added to adjust the pH to 4.0, and the extraction was carried out at room temperature for 24 hours, and then hydrochloric acid was added to adjust the pH to 4.0, and the mixture was heated to 75-85°C and kept warm for 30-60 minutes, and the precipitate was obtained by filtering;

[0036] Add 65% ethanol solution by volume to the precipitate, heat to 65-75° C., extract for 1.5-2 hours, filter, and obtain the supernatant, which is the witch hazel extract.

[0037] Specifically, the witch hazel is crushed and sieved to increase the contact area with the solvent and improve the extraction efficiency, and a 60-mesh screen is used for sieving.

[0038] The pretreatment process can effectively extract the active ingredients in Hamamelis virginiana, making it play a better role in anti-dandruff agents.

[0039] As a second aspect of the present invention, the use of the natural anti-dandruff agent as described in any one of the first aspects of the present invention in a hair product is claimed. The hair product may be shampoo, conditioner, scalp essence, hair mask, etc. The hair product may also include other auxiliary materials in hair products, such as water, surfactant, thickener, etc.

[0040] Furthermore, as a second aspect of the present invention, in the application, the amount of the natural anti-dandruff agent added to the shampoo accounts for 0.05% to 5% of the total amount of the shampoo.

[0041] When the natural anti-dandruff agent of the present invention is added to shampoo, the addition amount is 0.05% to 5% of the total amount of the shampoo, and can be 0.05%, 0.1%, 1%, 2%, 3%, 4%, 5%. Within this addition amount range, the composition can effectively play an anti-dandruff effect, and will not adversely affect other properties of the shampoo (such as foaming, stability, washing effect, etc.), providing consumers with a natural, safe and efficient anti-dandruff shampoo option.

[0042] Beneficial Effects

[0043] The natural anti-dandruff agent provided by the present invention contains carvacrol, alpine astragalus extract and North American witch hazel extract. The triple natural forces work synergistically. The composition has mild and antibacterial and anti-inflammatory effects. It not only effectively removes dandruff, but also promotes scalp health and restores natural balance.

[0044] The natural anti-dandruff agent of the present invention is used in hair products to prepare hair products which are less irritating, effectively remove dandruff and help improve scalp health. DETAILED DESCRIPTION

[0045] The embodiments of the present invention will be described in detail below in conjunction with the examples, but those skilled in the art will appreciate that the following examples are only used to illustrate the present invention and should not be considered to limit the scope of the present invention. If no specific conditions are specified in the examples, they are carried out according to normal conditions or the conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be obtained commercially.

[0046] The natural anti-dandruff agent of the present invention and its application will be described below in conjunction with embodiments and comparative examples.

[0047] Example 1

[0048] The natural anti-dandruff agent disclosed in Example 1 comprises, by weight, 0.1 parts of carvacrol, 2 parts of Astragalus membranaceus extract, and 2 parts of Hamamelis virginiana extract.

[0049] The pretreatment steps of carvacrol in Example 1 are as follows, including the steps of:

[0050] S101: Chop and grind caraway seeds, sieve through a 60-mesh sieve, and dry at 50° C. overnight, turning over to ensure complete drying, to obtain carvacrol powder.

[0051] S102: The carbon dioxide is pressurized to 7.50 MPa in a supercritical state, and heated to 50° C. to infiltrate the plant material.

[0052] S103: Supercritical CO2 interacts with carvacrol and other compounds in the carvacrol powder for 70 minutes to dissolve these components.

[0053] S104: reducing the pressure to 0.1 MPa to restore the supercritical CO2 to a gaseous state, and carvacrol and other components are released, and carvacrol is collected in a separator.

[0054] In Example 1, the pretreatment steps of the Astragalus membranaceus extract are:

[0055] S1011: Wash the Astragalus membranaceus, sieve it through a 60-mesh sieve, and dry it at 45°C overnight, turning it over to ensure complete drying, to obtain Astragalus membranaceus powder.

[0056] S1012: Adjust the equipment at a pressure of 50 MPa and a temperature of 55°C.

[0057] The alpine astragalus powder obtained after pretreatment was placed in an extractor and supercritical CO2 was added as an extractant. During the extraction process, the CO2 flow rate was adjusted to 3.7L / H to ensure sufficient dissolution and mass transfer. At the same time, the entrainer was 95% ethanol to improve the extraction efficiency and selectivity.

[0058] S1013: When the CO2 fluid saturated with dissolved matter enters the separator, the pressure drops to 0.1MPa, causing the CO2 and the extract to quickly become two phases and separate immediately, and the extracted Astragalus membranaceus extract is collected.

[0059] In Example 1, the pretreatment steps of the witch hazel extract are:

[0060] S10111: Crush the witch hazel and sieve it through a 60-mesh sieve to increase the contact area with the solvent and improve the extraction efficiency.

[0061] S10112: Add boric acid and extract at 65°C for 2 hours. Filter and take the supernatant, add citric acid to adjust the pH to 4.0, and extract at room temperature for 24 hours.

[0062] S10113: Then add hydrochloric acid to adjust the pH to 4.0, heat to 85°C and keep warm for 30 minutes, and filter to obtain the precipitate.

[0063] S10114: Add 65% ethanol solution by volume to the precipitate, extract at 70° C. for 2 hours, and filter to obtain the witch hazel extract.

[0064] Wherein, the natural anti-dandruff agent in Example 1 is prepared by the following steps:

[0065] S01: The pre-treated crude powder of Astragalus membranaceus extract and carvacrol are added to the North American witch hazel extract, and the ultrasonic assisted extraction technology is used to destroy the cell wall by the cavitation effect of ultrasound, thereby improving the dissolution rate of the active ingredients.

[0066] S02: After the extraction is completed, the solid residue is removed by centrifugal separation technology to obtain a liquid extract containing active ingredients.

[0067] S03: Then, membrane separation technology is used to further purify the extract to remove small molecule impurities and ineffective components.

[0068] S04: The purified extract is concentrated by low-temperature vacuum to reduce the water content and increase the concentration of active ingredients. Finally, the concentrate is converted into a powdered product that is easy to store through spray drying or freeze drying technology.

[0069] Example 2

[0070] The natural anti-dandruff agent disclosed in Example 2 includes, by weight, 0.1 parts of carvacrol, 2 parts of Astragalus membranaceus extract, and 2 parts of Hamamelis virginiana extract.

[0071] The pretreatment steps of carvacrol in Example 2 are as follows, including the steps of:

[0072] S201: Chop the caraway seeds, grind them, sieve them through a 60-mesh sieve, and dry them at 50° C. overnight, turning them over to ensure complete drying, to obtain carvacrol powder.

[0073] S202: The carbon dioxide is pressurized to 7.50 MPa in a supercritical state and heated to 40° C. to allow it to penetrate the plant material.

[0074] S203: Supercritical CO2 interacts with carvacrol and other compounds in the carvacrol powder for 50 minutes to dissolve these components.

[0075] S204: reducing the pressure to 0.1 MPa to restore the supercritical CO2 to a gaseous state, and carvacrol and other components are released, and carvacrol is collected in a separator.

[0076] In Example 2, the pretreatment steps of the Astragalus membranaceus extract are:

[0077] S2011: Wash the Astragalus membranaceus, sieve it through a 60-mesh sieve, and dry it at 45°C overnight, turning it over to ensure complete drying, to obtain Astragalus membranaceus powder.

[0078] S2012: The equipment is adjusted at a pressure of 40 MPa and a temperature of 45°C.

[0079] The alpine astragalus powder obtained after pretreatment was placed in an extractor and supercritical CO2 was added as an extractant. During the extraction process, the CO2 flow rate was adjusted to 3.7L / H to ensure sufficient dissolution and mass transfer. At the same time, the entrainer was 95% ethanol to improve the extraction efficiency and selectivity.

[0080] S2013: When the CO2 fluid saturated with dissolved matter enters the separator, the pressure drops to 0.1MPa, causing the CO2 and the extract to quickly become two phases and separate immediately, and the extracted Astragalus membranaceus extract is collected.

[0081] In Example 2, the pretreatment steps of the witch hazel extract are:

[0082] S20111: Crush the witch hazel and sieve it through a 60-mesh sieve to increase the contact area with the solvent and improve the extraction efficiency.

[0083] S20112: Add boric acid and extract at 65°C for 1.5 hours. Filter and take the supernatant, add citric acid to adjust the pH to 4.0, and extract at room temperature for 24 hours.

[0084] S20113: Then add hydrochloric acid to adjust the pH to 4.0, heat to 80°C and keep warm for 30 minutes, and filter to obtain the precipitate.

[0085] S20114: Add 65% ethanol solution by volume to the precipitate, extract at 70° C. for 1.5 hours, and filter to obtain the witch hazel extract.

[0086] Wherein, the preparation method of the natural anti-dandruff agent in Example 2 is the same as that in Example 1.

[0087] Example 3

[0088] The natural anti-dandruff agent disclosed in Example 3 comprises, by weight, 0.1 parts of carvacrol, 2 parts of Astragalus membranaceus extract, and 2 parts of Hamamelis virginiana extract.

[0089] The pretreatment steps of carvacrol in Example 3 are as follows, including the steps of:

[0090] S301: Chop the caraway seeds, grind them, sieve them through a 60-mesh sieve, and dry them at 50° C. overnight, turning them over to ensure complete drying, to obtain carvacrol powder.

[0091] S302: The carbon dioxide is pressurized to 7.50 MPa in a supercritical state and heated to 30°C to allow it to penetrate the plant material.

[0092] S303: Supercritical CO2 interacts with carvacrol and other compounds in the carvacrol powder for 30 minutes to dissolve these components.

[0093] S304: reducing the pressure to 0.1 MPa to restore the supercritical CO2 to a gaseous state, and carvacrol and other components are released, and carvacrol is collected in a separator.

[0094] In Example 3, the pretreatment steps of the Astragalus membranaceus extract are:

[0095] S3011: Wash the Astragalus membranaceus, sieve it through a 60-mesh sieve, and dry it at 45°C overnight, turning it over to ensure complete drying, to obtain Astragalus membranaceus powder.

[0096] S3012: Adjust the equipment at a pressure of 30 MPa and a temperature of 30°C.

[0097] The alpine astragalus powder obtained after pretreatment was placed in an extractor and supercritical CO2 was added as an extractant. During the extraction process, the CO2 flow rate was adjusted to 3.7L / H to ensure sufficient dissolution and mass transfer. At the same time, the entrainer was 95% ethanol to improve the extraction efficiency and selectivity.

[0098] S3013: When the CO2 fluid saturated with dissolved matter enters the separator, the pressure drops to 0.1MPa, causing the CO2 and the extract to quickly become two phases and separate immediately, and the extracted Astragalus membranaceus extract is collected.

[0099] In Example 3, the pretreatment steps of the witch hazel extract are:

[0100] S30111: Crush the witch hazel and sieve it through a 60-mesh sieve to increase the contact area with the solvent and improve the extraction efficiency.

[0101] S30112: Add boric acid and extract at 65°C for 1 hour. Filter and take the supernatant, add citric acid to adjust the pH to 4.0, and extract at room temperature for 24 hours.

[0102] S30113: Then add hydrochloric acid to adjust the pH to 4.0, heat to 80°C and keep warm for 30 minutes, and filter to obtain the precipitate.

[0103] S30114: Add 65% ethanol solution by volume to the precipitate, extract at 70° C. for 1.5 hours, and filter to obtain the witch hazel extract.

[0104] Wherein, the preparation method of the natural anti-dandruff agent in Example 3 is the same as that in Example 1.

[0105] Example 4

[0106] The natural anti-dandruff agent disclosed in Example 4 comprises, by weight, 0.1 parts of carvacrol, 5 parts of Astragalus membranaceus extract, and 0.2 parts of Hamamelis virginiana extract.

[0107] The natural anti-dandruff agent in Example 4 has the same pretreatment steps as those in Example 1, and the preparation method of the natural anti-dandruff agent is the same as that in Example 1.

[0108] Example 5

[0109] The natural anti-dandruff agent disclosed in Example 5 includes, by weight, 0.1 parts of carvacrol, 0.2 parts of Astragalus membranaceus extract, and 5 parts of Hamamelis virginiana extract.

[0110] The pretreatment steps of the components of the natural anti-dandruff agent in Example 5 are the same as those in Example 1, and the preparation method of the natural anti-dandruff agent is the same as that in Example 1.

[0111] The natural anti-dandruff agent formulations in Examples 1-5 are shown in Table 1 below.

[0112] Table 1, Example natural antidandruff agent ratio

[0113]

[0114] In order to verify the effect of the embodiments of the present invention, the present invention also provides 8 comparative examples for comparative explanation, and the natural anti-dandruff agent formulations in the comparative examples are shown in Table 2 below. In the comparative examples, other natural plants are provided as comparisons of effective anti-dandruff components, and other natural anti-dandruff agents include inulin and Melaleuca alternifolia leaves.

[0115] The pretreatment steps and preparation method of the natural anti-dandruff agent in the comparative example are the same as those in Example 1. Inulin and Melaleuca alternifolia leaves are purchased from the market.

[0116] Table 2, Comparative Example Natural Composition Ratio Table

[0117]

[0118] The specific embodiment of the present invention also provides a specific application example of a natural anti-dandruff agent, specifically a shampoo. In the specific embodiment of the present invention, the corresponding test and effect will be specifically described in combination with its application. The formula of the shampoo is shown in Table 3 below. The mass fraction of the natural anti-dandruff agent in the shampoo is 2%.

[0119] Table 3. Shampoo formula

[0120]

[0121]

[0122] The preparation steps of the shampoo are as follows:

[0123] S1: In the first container, add an appropriate amount of deionized water (76.9% of the total amount), and then add other components of phase A in sequence, stirring and heating until they are completely dissolved.

[0124] S2: In the first container, measure the remaining deionized water (18% of the total amount), add the other components of phase B in sequence, and stir and heat until they are completely dissolved.

[0125] S3: Slowly pour solution B into solution A while continuing to stir to ensure uniform mixing and cool to room temperature.

[0126] S4: Add the components of phase C and phase D to the mixed solution in sequence, stirring thoroughly after each addition to ensure that the components are evenly dispersed or dissolved.

[0127] S5: After passing the inspection, the material is discharged to obtain shampoo.

[0128] The first container and the second container can be selected as a beaker, a storage tank, a stirring kettle, etc. according to the preparation quantity requirements, and are not limited here.

[0129] Test experiment 1: Test on the effect of inhibiting Malassezia

[0130] Experimental Materials

[0131] Malassezia strain: Use standard strain.

[0132] Culture medium: Sabouraud medium containing olive oil and Tween 80.

[0133] Test samples: the natural anti-dandruff shampoo used in Examples 1-5 and Comparative Examples 1-8. Control samples: the control group is shampoo without adding natural anti-dandruff agent.

[0134] Experimental equipment

[0135] Biological safety cabinet: used for sterile operation; incubator: used for bacterial culture; centrifuge: used for bacterial liquid processing; microscope: used to observe bacterial morphology and quantity; counter: used to calculate the number of bacteria.

[0136] Experimental methods

[0137] Strain preparation: Inoculate the Malassezia strain onto the culture medium and culture to the logarithmic growth phase.

[0138] Preparation of bacterial solution: The Malassezia strain in the logarithmic growth phase was diluted with sterile saline to a bacterial solution of 10^9 CFU / m.

[0139] Grouping and treatment: Divide the bacterial solution into a control group and an experimental group. Add the test substance to the experimental group and the control substance to the control group. Ensure that the concentration of bacterial solution in each group is consistent.

[0140] Cultivation and observation: Inoculate the bacterial solution of each group onto the culture medium and place it in an incubator for cultivation. Then observe the growth of the colonies in each group and record the number of colonies (logarithm) at 0, 24, 48, and 72 hours.

[0141] The test results are shown in Table 4 below:

[0142] Table 4. Malassezia test results

[0143]

[0144] It can be seen from Table 4 that the natural anti-dandruff agent of the present invention has the effect of inhibiting Malassezia. It can be seen from Application Examples 1 to 5 that the pretreatment steps and the ratio of the composition in the natural anti-dandruff agent can affect the antibacterial effect of Malassezia, and the inhibitory effect on Malassezia can be improved by adjusting the experimental parameters. By comparing Comparative Examples 1 to 6, it can be seen that carvacrol plays a major role in the inhibitory effect on Malassezia, and the alpine astragalus extract and the North American witch hazel extract play an auxiliary and synergistic role. It can be seen from Comparative Examples 7 and 8 that the natural anti-dandruff agent of the present invention has certain advantages over other plant anti-dandruff agents.

[0145] Test experiment 2: Anti-dandruff human efficacy test

[0146] Test population: 65 volunteers, aged between 18 and 60 years old.

[0147] Scalp condition: All volunteers had mild to moderate dandruff problems.

[0148] Exclusion criteria: known allergy to shampoo ingredients, skin diseases, pregnant or lactating women.

[0149] Test Method

[0150] Initial Assessment: Dandruff was scored using a standardized dandruff scoring system ranging from 0 (no dandruff) to 5 (extremely severe dandruff).

[0151] Grouping and processing

[0152] Random grouping: Randomly assign and use the natural anti-dandruff shampoo corresponding to the application examples of Examples 1-5 and Comparative Examples 1-8, and 5 people use each shampoo.

[0153] Usage: 65 volunteers were tested on half of their heads, with one side using the assigned natural anti-dandruff shampoo and the other side using a shampoo without added natural anti-dandruff agents, washing their hair once a day for 4 weeks.

[0154] Regular evaluation: record the dandruff score every week and observe the number, size and distribution of dandruff. Final evaluation: make a final score at the end of the 4th week and record the changes in dandruff.

[0155] Test Results

[0156] The amount of dandruff is calculated as follows: select the area with the most severe dandruff and score it according to the number of dandruff flakes per 1 square centimeter of the scalp area.

[0157] Table 5. Dandruff quantity table

[0158]

[0159] The test score results are shown in Table 6 below:

[0160] Table 6. Scoring test table

[0161]

[0162] It can be concluded from Table 5 and Table 6 that the natural anti-dandruff agent of the embodiment of the present invention has more advantages than the comparative example.

[0163] In summary, the natural anti-dandruff agent of the present invention can effectively inhibit the growth of harmful microorganisms that cause dandruff on the scalp, and has a soothing effect that can improve the ecological environment of the scalp and promote scalp health. The various ingredients in the natural anti-dandruff agent work synergistically and are indispensable to each other, and together they exert a good anti-dandruff effect.

[0164] The above embodiments are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the claims of the present invention.

Claims

1. A natural anti-dandruff agent, characterized in that: Calculated by weight, the invention comprises: 0.01 to 5 parts of carvacrol, 0.01 to 10 parts of alpine astragalus extract, and 0.01 to 10 parts of North American witch hazel extract.

2. The natural anti-dandruff agent according to claim 1, characterized in that Calculated by weight, the invention comprises: 0.1-0.2 parts of carvacrol, 0.2-5 parts of alpine astragalus extract, and 0.2-5 parts of witch hazel extract.

3. The natural anti-dandruff agent according to claim 2, characterized in that: Calculated by weight, the invention comprises: 0.1-0.2 parts of carvacrol, 1-5 parts of alpine astragalus extract and 0.2-5 parts of witch hazel extract.

4. The natural anti-dandruff agent according to claim 3, characterized in that Calculated by weight, the invention comprises: 0.1-0.2 parts of carvacrol, 1-5 parts of alpine astragalus extract and 1-5 parts of witch hazel extract.

5. The natural anti-dandruff agent according to any one of claims 1 to 4, characterized in that: The preparation method of the natural anti-dandruff agent comprises the following steps: S1, adding alpine astragalus extract and carvacrol to witch hazel extract in proportion, and using ultrasound-assisted extraction; S2. After the extraction is completed, the solid residue is removed by centrifugation to obtain a liquid extract containing active ingredients; S3, filtering and purifying the liquid extract by membrane separation; S4. The purified extract is concentrated by low-temperature vacuum concentration, and then the concentrate is converted into a powdered product by spray drying or freeze drying technology.

6. The natural anti-dandruff agent according to claim 5, characterized in that: The carvacrol is obtained by processing the caraway seeds to obtain carvacrol powder, and the carvacrol powder is extracted with supercritical carbon dioxide to obtain carvacrol, and the extraction conditions are heating to 30-50° C. and pressurizing to above 7.50 MPa.

7. The natural anti-dandruff agent according to any one of claim 5, characterized in that: The pretreatment steps of the alpine astragalus extract include washing, drying, crushing and screening the alpine astragalus, and then extracting the alpine astragalus extract using supercritical carbon dioxide, the extraction temperature is 30-55°C, the extraction pressure is 30-50MPa, and the entrainer is 95% ethanol.

8. The natural anti-dandruff agent according to claim 5, characterized in that The pretreatment step of the witch hazel extract comprises: The North American witch hazel was crushed and sieved, and after adding boric acid, it was extracted at 60-70°C for 1-2 hours, and the supernatant was taken after filtering, and citric acid was added to adjust the pH to 4.0, and the extraction was carried out at room temperature for 24 hours, and then hydrochloric acid was added to adjust the pH to 4.0, and the mixture was heated to 75-85°C and kept warm for 30-60 minutes, and the precipitate was obtained by filtering; Add 65% ethanol solution by volume to the precipitate, heat to 65-75° C., extract for 1.5-2 hours, filter, and obtain the supernatant, which is the witch hazel extract.

9. Use of the natural anti-dandruff agent according to any one of claims 1 to 8 in hair products.

10. The use according to claim 9, characterized in that: In shampoo, the amount of the natural anti-dandruff agent added accounts for 0.05% to 5% of the total amount of shampoo.

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