Synergistic repair composition as well as preparation method and application thereof

Abstract

The invention discloses a synergistic repairing composition as well as a preparation method and application thereof, and relates to the technical field of cosmetics. The synergistic repairing composition is prepared from the following components: fibronectin, soluble proteoglycan and an artemia extract. The invention provides a synergistic repair composition as well as a preparation method and application thereof, the synergistic repair composition has a strong repair effect, after fibronectin, soluble proteoglycan and artemia (ARTEMIA) extract are compounded, a synergistic effect on the repair effect can be obtained, and the synergistic repair composition has a stronger repair effect than any component independently used or a component formed by combining every two components.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a synergistic repairing composition and a preparation method and application thereof. Background Art

[0002] As the first line of defense for the human body against the external environment, the skin protects the human body from various physical, chemical and microbial factors. However, genetics, physical or chemical stimulation, age, environmental factors, diseases and drugs may cause damage to the skin barrier. A damaged skin barrier reduces the body's protective ability, thereby inducing inflammation. Inflammation promotes the appearance of symptoms such as redness and aging. Therefore, repairing the skin barrier has become one of the main research directions of cosmetics.

[0003] Fibronectin (FN) is a structural protein in the human body with a molecular weight of 450kd. It is widely present in tissues and tissue fluids and is widely used in regenerative medicine. It plays an important role in the hemostasis-inflammation-proliferation-remodeling process of wound healing. In the hemostasis stage, FN helps to form blood clots and assemble the extracellular matrix (ECM); in the inflammation stage, FN itself can clean up ECM fragments, and it can also recruit and activate macrophages so that they can phagocytize these fragments at the wound site; in the proliferation stage, FN is the main channel for cell migration, suitable for keratinocytes and fibroblasts; in the remodeling stage, ECM reconstruction is a complex and regulated process. FN updates and remodels, regulating the transition of ECM structure from temporary to permanent ECM structure.

[0004] From the above, we can see that fibronectin has the biological properties of promoting cell migration, repairing damaged cells, activating cells with insufficient vitality, and promoting cell proliferation. Damage to the skin barrier is associated with decreased tight junctions of stratum corneum cells, decreased cell vitality, and decreased migration ability. Therefore, combined with the efficacy of FN, it can be used in repair cosmetics to enhance the skin barrier, promote cell proliferation and migration, and achieve the effect of quickly repairing damaged skin.

[0005] Artemia extract is derived from the primitive marine plankton Artemia, and its core component is diguanosine tetraphosphate, which can regulate the expression of heat shock protein HSP70 and promote the production of collagen and fibronectin in fibroblasts. Studies have shown that the expression of heat shock protein can promote fibronectin activity; in addition, Artemia extract also has the effect of promoting cell vitality and repairing damaged cells, and is expected to have a synergistic repair effect with fibronectin.

[0006] Soluble proteoglycans are covalent bonds composed of amino polysaccharides and proteins. In the skin, proteoglycans are secreted by fibroblasts and widely distributed in the dermis. Their main function is to serve as a matrix for embedding or filling collagen and elastin, playing roles such as filling, elasticity, water retention, and lubrication. At the same time, they can also promote the synthesis of collagen I and the proliferation and migration of epidermal cells. Studies have shown that soluble proteoglycans can promote the activity of fibronectin receptors and are also expected to have a synergistic repair effect with fibronectin.

[0007] Many existing cosmetics achieve the effect of repairing the skin by exogenously supplementing the physical and chemical barrier components of the skin. However, as consumers have higher and higher expectations for repair products, it is not enough to rely solely on exogenous supplementation of the physical and chemical barrier, so a composition that promotes self-repair ability is needed to meet the needs of consumers in the new era. Based on this, the present invention provides a synergistic repair composition and its preparation method and application, which uses fibronectin as the main body, compounded with soluble proteoglycan and Artemia extract, to enhance the repair effect of fibronectin. Summary of the invention

[0008] In order to solve the above technical problems, the present invention provides a synergistic repair composition and its preparation method and application. Specifically, it includes the following technical solutions:

[0009] In a first aspect, a synergistic repair composition is provided, comprising the following components: fibronectin, soluble proteoglycan, and Artemia extract.

[0010] Furthermore, the weight percentage of the fibronectin in the synergistic repair composition is 0.01%-2%.

[0011] Preferably, the fibronectin is a product of Melgene (Shenzhen) Biotechnology Co., Ltd. under the trade name of MELLPRO 500G+Fibronectin Solution 1.01.

[0012] Furthermore, the weight percentage of the soluble proteoglycan in the synergistic repair composition is 0.001%-0.5%.

[0013] Preferably, the soluble proteoglycan is from Runhui Biotechnology (Weihai) Co., Ltd. under the trade name Soluble proteoglycan products.

[0014] Furthermore, the weight percentage of the Artemia extract in the synergistic repair composition is 0.1%-2%.

[0015] Preferably, the Artemia extract is Artemia extract.

[0016] Preferably, the Artemia extract is from Ashland LLC and is marketed under the trade name gp4gTM sp biofunctional products.

[0017] Furthermore, the synergistic repair composition is composed of the following components in weight percentage: 0.01%-2% fibronectin, 0.001%-0.5% soluble proteoglycan, 0.1%-2% Artemia extract, and the balance is deionized water.

[0018] Furthermore, the weight percentage of the fibronectin in the synergistic repair composition is 0.025%-0.2%.

[0019] Furthermore, the weight percentage of the soluble proteoglycan in the synergistic repair composition is 0.00125%-0.00625%.

[0020] Furthermore, the weight percentage of the Artemia extract in the synergistic repair composition is 0.125%-1%.

[0021] In a second aspect, a method for preparing the synergistic repair composition as described in the first aspect is provided, comprising the following steps: mixing soluble proteoglycan with deionized water, stirring to dissolve, adding fibronectin and Artemia extract in sequence, stirring evenly, and obtaining the synergistic repair composition.

[0022] In a third aspect, there is provided use of the synergistic repair composition as described in the first aspect in repair cosmetics.

[0023] The beneficial effects of the present invention are:

[0024] The present invention provides a synergistic repair composition and a preparation method and application thereof, which has a strong repair effect. After compounding fibronectin, soluble proteoglycan and Artemisia (ARTEMIA) extract, a synergistic enhancement in the repair effect can be obtained, and the repair effect is stronger than that of using any component alone or the components in combination of two. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying any creative work.

[0026] Figure 1 These are the test results of promoting barrier gene expression in Examples 1-2 and Comparative Examples 1-8 of the present invention;

[0027] Figure 2 The cell proliferation test results of the synergistic repair compositions of Examples 2-3 and Comparative Examples 5-12 of the present invention;

[0028] Figure 3 These are the results of cell scratch promotion tests of the synergistic repair compositions of Example 1, Example 4, Comparative Examples 1-4, and Comparative Examples 13-16 of the present invention. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] It should be understood that when used in this specification and the appended claims, the terms "include" and "comprises" indicate the presence of described features, integers, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or combinations thereof.

[0031] It should also be understood that the terms used in this specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. As used in the specification of the present invention and the appended claims, unless the context clearly indicates otherwise, the singular forms "a", "an" and "the" are intended to include plural forms.

[0032] It should be further understood that the term "and / or" used in the present description and the appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes these combinations.

[0033] In order to more fully understand the technical content of the present invention, the technical solution of the present invention is further introduced and illustrated in conjunction with specific embodiments below.

[0034] The fibronectin described in the examples of the present invention and the comparative examples is a product of MELLPRO 500G+fibronectin solution 1.01 produced by Meierjian (Shenzhen) Biotechnology Co., Ltd. The soluble proteoglycan described in the examples of the present invention and the comparative examples is a product of Runhui Biotechnology (Weihai) Co., Ltd. The Artemia extracts described in the examples and comparative examples of the present invention are from Ashland LLC and are marketed under the trade name gp4g TM spbiofunctional products.

[0035] The preparation method of the synergistic repair composition comprises the following steps:

[0036] The soluble proteoglycan is mixed with deionized water, stirred to dissolve, and then fibronectin and Artemia extract are added in sequence and stirred evenly to obtain a synergistic repair composition.

[0037] Performance test of synergistic repair composition:

[0038] 1. Promote barrier gene expression test (IVL gene expression test)

[0039] 1.1 Experimental Principle:

[0040] In the process of keratinocytes differentiating upward to form the skin barrier, various structural genes will be expressed and translated into proteins to varying degrees and specifically distributed in various layers of the skin. The proteins closely related to the skin barrier function are mainly related to the tight junction proteins between cells and the anchoring junction proteins between cells and the matrix. Involucrin (IVL) is mainly expressed in the upper part of the spinous cell layer and the granular layer. It is a marker protein for keratinocyte differentiation. It is located in the outer layer of the cornified envelope (CE) and covalently binds to ceramides containing -OH, thereby connecting the lipid matrix and keratinocytes to play a role. This experiment uses human immortalized keratinocytes (HaCaT) to detect the relative expression of involucrin (IVL) after the sample acts on the cells to evaluate the barrier repair effect of the sample.

[0041] 1.2 Test method:

[0042] 1.2.1 Cell inoculation: HaCaT cells were inoculated in 6-well plates, 6 × 10 cells per well, and cultured at 37°C, CO2 for 24 h.

[0043] 1.2.2 Administration: After incubation, the cells were gently rinsed once or twice with D-Hanks, fresh culture medium was added to the normal control group, and fresh culture medium containing samples of corresponding concentrations was added to the sample group, and cultured at 37°C, 5% CO2 for 24 h.

[0044] 1.2.3 Sampling and detection: After the culture is completed, the cells are sampled, the total RNA of each experimental group is extracted, cDNA is synthesized, and the gene expression of β-actin and the target gene is detected by q-PCR.

[0045] 1.2.4 Data analysis: β-actin was used as an internal reference for gene expression to calculate the relative RNA expression of the target gene.

[0046] 2. Cell proliferation test (cell proliferation rate test)

[0047] 2.1 Experimental principle: CCK-8 contains WST-8 [chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfonic acid benzene)-2H-tetrazolyl monosodium salt], which is reduced to a highly water-soluble yellow formazan product (Formazan dye) by dehydrogenase in cells under the action of the electron carrier 1-methoxy-5-methylphenazinium dimethyl sulfate (1-Methoxy PMS). The amount of formazan generated is proportional to the number of living cells. Therefore, this property can be used to directly perform cell proliferation and toxicity analysis.

[0048] 2.2 Experimental methods:

[0049] 2.2.1 Preparation of cell suspension: Cell counting.

[0050] 2.2.2 Inoculate into 96-well plate: Based on the appropriate number of cells to be plated, add about 100 μL of cell suspension to each well. The same sample can be replicated three times.

[0051] 2.2.3 Culture in a 37°C incubator: It takes about 2 to 4 hours for cells to adhere to the wall after inoculation. If adhesion is not required, this step can be omitted.

[0052] 2.2.4 Add 10μL CCK8: Since the amount of CCK8 added to each well is relatively small, it may cause errors due to the reagent sticking to the well wall. It is recommended to gently tap the culture plate after adding the reagent to help mix it. Alternatively, directly prepare a culture medium containing 10% CCK8 and add it in the form of liquid replacement.

[0053] 2.2.5 Culture for 1-4 hours: Different cell types will produce different amounts of formazan. If the color development is not enough, continue to culture to confirm the optimal conditions. In particular, blood cells produce very little formazan and require a longer color development time (5-6 hours).

[0054] 2.2.6 Determination of absorbance at 450nm: It is recommended to use dual wavelength for determination, with the detection wavelength of 450~490nm and the reference wavelength of 600~650nm.

[0055] 3. Promote cell scratch test (cell migration test)

[0056] 3.1 Experimental principle: When the cells grow to the point where they fuse into a monolayer, use a micropipette tip or other hard objects to artificially create a blank area on the fused monolayer of cells, which is called a "scratch". Then continue to culture the cells until the time set in the experiment. The cells at the edge of the scratch will leave the crowded area and gradually enter the blank area, thereby healing the "scratch". The experimenter observes under a microscope and takes pictures to record whether the cells at a specific location have migrated to the "scratch area" to determine the cell's ability to grow and migrate. The scratch experiment simulates the process of cell migration in vivo to a certain extent. The differences in the ability of cells in different groups to repair the scratch area can be used to determine the differences in the migration and repair abilities of each group of cells.

[0057] 3.2 Experimental steps:

[0058] (1) Marking: On the bottom of the six-well plate, use a marker to draw three horizontal lines along the ruler as marking lines;

[0059] (2) Plating: Plating cells according to grouping, with a cell density of about 5 x 105 cells / well, shaking to spread the cells evenly;

[0060] (3) Scratching: After the cells have grown fully (about 24 hours later), use the tip of a white gun to draw three vertical lines perpendicular to the well plate and the marked line, so that the scratches intersect with the marked lines, forming 16 areas, and select 9 areas as fixed detection points;

[0061] (4) Washing: Discard the old culture medium and gently rinse with PBS two to three times until the cells are washed clean;

[0062] (5) Adding liquid: adding drug-added culture medium or serum-free culture medium according to the group;

[0063] (6) Photographing and observing: After scratching, washing, and adding liquid, take a 10X photo under a microscope as a 0h control. Place in a 37°C, 5% CO2 incubator for incubation. Take out the cells at 12h, 24h, 36h, 48h, etc., observe the width of the scratch at the same position under a microscope and take photos until a group of cells has grown all over the scratch;

[0064] (7) Data analysis: ImageJ software can be used to analyze the cell migration distance and scratch area.

[0065] 1. Influence based on synergistic effects between components

[0066] According to the formula composition in Table 1, the synergistic repair compositions of Examples 1-2 and Comparative Examples 1-8 were prepared.

[0067] Table 1 Formula composition of Examples 1-2 and Comparative Examples 1-8 (weight percentage, %)

[0068] Example / Comparative Example Fibronectin Soluble proteoglycan Artemia Extract Deionized water Example 1 0.05％ 0.00125％ 0.25％ margin Comparative Example 1 0.05％ / / margin Comparative Example 2 / / 0.25％ margin Comparative Example 3 / 0.00125％ / margin Comparative Example 4 0.05％ / 0.25％ margin Example 2 0.1％ 0.0025％ 0.5％ margin Comparative Example 5 0.1％ / / margin Comparative Example 6 / / 0.5％ margin Comparative Example 7 / 0.0025％ / margin Comparative Example 8 0.1％ / 0.5％ margin

[0069] Among them, the preparation method of the synergistic repair composition of Example 1 includes the following steps: mixing soluble proteoglycan with deionized water, stirring to dissolve, adding fibronectin and Artemia extract in sequence, stirring evenly, and then obtaining the synergistic repair composition.

[0070] The preparation methods of the synergistic repair compositions of Example 2 and Comparative Examples 1-8 are the same as that of Example 1.

[0071] The synergistic repair compositions of Examples 1-2 and Comparative Examples 1-8 were tested for promoting barrier gene expression. The test results are shown in Table 2:

[0072] Table 2 Test results of promoting barrier gene expression in Examples 1-2 and Comparative Examples 1-8

[0073] Example / Comparative Example IVL gene expression Example 1 1.177 Comparative Example 1 0.798 Comparative Example 2 1.017 Comparative Example 3 0.996 Comparative Example 4 0.980 Example 2 1.264 Comparative Example 5 0.723 Comparative Example 6 0.992 Comparative Example 7 1.028 Comparative Example 8 1.050

[0074] Figure 1 The test results of promoting barrier gene expression in Examples 1-2 and Comparative Examples 1-8 of the present invention are shown in FIG. Figure 1 As shown in the test results of Table 2, the test results of promoting barrier gene expression of the synergistic repair compositions (Comparative Examples 1-8) lacking one or more components are poor. This is because the present invention improves the barrier repair effect through the synergistic effect of fibronectin, soluble proteoglycan and Artemia extract.

[0075] According to the formula composition in Table 3, the synergistic repair compositions of Example 3 and Comparative Examples 9-12 were prepared.

[0076] Table 3 Formula composition of Example 3 and Comparative Examples 9-12 (weight percentage, %)

[0077]

[0078]

[0079] The preparation methods of the synergistic repair compositions of Example 3 and Comparative Examples 9-12 are the same as that of Example 1.

[0080] The synergistic repair compositions of Examples 2-3 and Comparative Examples 5-12 were subjected to a cell proliferation test, and the test results are shown in Table 4:

[0081] Table 4 Cell proliferation test results of Examples 2-3 and Comparative Examples 5-12

[0082] Example / Comparative Example Cell proliferation rate, % Example 2 117.353 Comparative Example 5 106.923 Comparative Example 6 108.927 Comparative Example 7 104.047 Comparative Example 8 110.278 Example 3 118.277 Comparative Example 9 103.543 Comparative Example 10 112.827 Comparative Example 11 109.583 Comparative Example 12 113.475

[0083] Figure 2The cell proliferation test results of the synergistic repair compositions of Examples 2-3 and Comparative Examples 5-12 of the present invention are shown in FIG. Figure 2 As shown in the test results of Table 4, the cell proliferation rate of the synergistic repair composition lacking a single component or prepared by multiple components is low. This is because the present invention can promote cell proliferation through the synergistic effect of fibronectin, soluble proteoglycan and Artemia extract, thereby improving the barrier repair effect.

[0084] According to the formula composition in Table 5, the synergistic repair compositions of Example 4 and Comparative Examples 13-16 were prepared.

[0085] Table 5 Formula composition of Example 4 and Comparative Examples 13-16 (weight percentage, %)

[0086]

[0087]

[0088] The preparation methods of the synergistic repair compositions of Example 4 and Comparative Examples 13-16 are the same as that of Example 1.

[0089] The synergistic repair compositions of Example 1, Example 4, Comparative Examples 1-4, and Comparative Examples 13-16 were subjected to a cell scratch promotion test. The test results are shown in Table 6:

[0090] Table 6 Results of cell scratch promotion test of Example 1, Example 4, Comparative Examples 1-4, Comparative Examples 13-16

[0091] Example / Comparative Example Cell migration rate, % Example 1 56.54 Comparative Example 1 43.76 Comparative Example 2 0.47 Comparative Example 3 27.45 Comparative Example 4 45.60 Example 4 60.64 Comparative Example 13 40.49 Comparative Example 14 0.98 Comparative Example 15 31.17 Comparative Example 16 42.38

[0092] Figure 3 The synergistic repair compositions of Example 1, Example 4, Comparative Examples 1-4, and Comparative Examples 13-16 of the present invention promote cell scratch test results. From the test results of Table 6, it can be seen that the cell migration rate of the synergistic repair compositions lacking a single component or prepared from multiple components is low. This is because the present invention can increase the migration and repair ability of cells through the synergistic effect of fibronectin, soluble proteoglycan, and Artemia extract, thereby improving the barrier repair effect.

[0093] 2. Effect of fibronectin weight percentage

[0094] According to the formula composition in Table 7, the synergistic repair compositions of Example 5 and Comparative Examples 17-18 were prepared.

[0095] Table 7 Formula composition of Example 5 and Comparative Examples 17-18 (weight percentage, %)

[0096] Example / Comparative Example Fibronectin Soluble proteoglycan Artemia Extract Deionized water Example 5 0.01％ 0.001％ 0.1％ margin Comparative Example 17 0.005％ 0.001％ 0.1％ margin Comparative Example 18 2.5％ 0.001％ 0.1％ margin

[0097] The preparation methods of the synergistic repair compositions of Example 5 and Comparative Examples 17-18 are the same as that of Example 1.

[0098] The synergistic repair compositions of Example 5 and Comparative Examples 17-18 were tested for promoting barrier gene expression. The test results are shown in Table 8:

[0099] Table 8 Test results of promoting barrier gene expression in Example 5 and Comparative Examples 17-18

[0100] Example / Comparative Example IVL gene expression Example 5 1.08 Comparative Example 17 0.87 Comparative Example 18 0.93

[0101] From the test results in Table 8, it can be seen that if the synergistic repair composition prepared by using fibronectin not within the scope of the present invention (Comparative Example 17, Comparative Example 18) has poor barrier repair effect. It can be seen that the weight percentage of fibronectin in the synergistic repair composition of the present invention is preferably 0.01%-2%.

[0102] 3. Effect of soluble proteoglycan weight percentage

[0103] According to the formula composition in Table 9, the synergistic repair compositions of Example 6 and Comparative Examples 19-20 were prepared.

[0104] Table 9 Formula composition of Example 6 and Comparative Examples 19-20 (weight percentage, %)

[0105] Example / Comparative Example Fibronectin Soluble proteoglycan Artemia Extract Deionized water Example 6 2％ 0.5％ 2％ margin Comparative Example 19 2％ 0.0005％ 2％ margin Comparative Example 20 2％ 0.6％ 2％ margin

[0106] The preparation methods of the synergistic repair compositions of Example 6 and Comparative Examples 19-20 are the same as that of Example 1.

[0107] The synergistic repair compositions of Example 6 and Comparative Examples 19-20 were subjected to a cell proliferation test. The test results are shown in Table 10:

[0108] Table 10 Cell proliferation test results of Example 6 and Comparative Examples 19-20

[0109] Example / Comparative Example Cell proliferation rate, % Example 6 107.4 Comparative Example 19 98.9 Comparative Example 20 91.2

[0110] From the test results in Table 10, it can be seen that if the synergistic repair composition (Comparative Example 19, Comparative Example 20) is prepared using soluble proteoglycans not within the scope of the present invention, the cell proliferation rate is low. It can be seen that the weight percentage of soluble proteoglycans in the synergistic repair composition of the present invention is preferably 0.001%-0.5%.

[0111] IV. Effect of weight percentage of Artemia extract

[0112] According to the formula composition in Table 11, the synergistic repair compositions of Example 7 and Comparative Examples 21-22 were prepared.

[0113] Table 11 Formula composition of Example 7 and Comparative Examples 21-22 (weight percentage, %)

[0114] Example / Comparative Example Fibronectin Soluble proteoglycan Artemia Extract Deionized water Example 7 1％ 0.25％ 1％ margin Comparative Example 21 1％ 0.25％ 0.05％ margin Comparative Example 22 1％ 0.25％ 2.5％ margin

[0115] The preparation methods of the synergistic repair compositions of Example 7 and Comparative Examples 21-22 are the same as that of Example 1.

[0116] The synergistic repair compositions of Example 7 and Comparative Examples 21-22 were subjected to a cell scratch promotion test. The test results are shown in Table 12:

[0117] Table 12 Results of cell scratch promotion test of Example 7 and Comparative Examples 21-22

[0118] Example / Comparative Example Cell migration rate, % Example 7 55.81 Comparative Example 21 40.62 Comparative Example 22 37.56

[0119] From the test results in Table 12, it can be seen that if the synergistic repair composition (Comparative Example 20, Comparative Example 21) is prepared using Artemia extracts not within the scope of the present invention, the cell migration rate is low. It can be seen that the weight percentage of Artemia extract in the synergistic repair composition of the present invention is preferably 0.1%-2%.

[0120] In summary, the present invention provides a synergistic repair composition and its preparation method and application, which has a strong repair effect. After compounding fibronectin, soluble proteoglycan, and Artemisia (ARTEMIA) extract, a synergistic enhancement in the repair effect can be obtained, which has a stronger repair effect than using any component alone or the components in combination of two.

[0121] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed by the present invention, and these modifications or replacements should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention shall be based on the protection scope of the claims.

Claims

1. A synergistic repair composition, characterized in that: The invention comprises the following components: fibronectin, soluble proteoglycan and Artemia extract.

2. The synergistic repair composition according to claim 1, characterized in that The weight percentage of the fibronectin in the synergistic repair composition is 0.01%-2%.

3. The synergistic repair composition according to claim 1, characterized in that The weight percentage of the soluble proteoglycan in the synergistic repair composition is 0.001%-0.5%.

4. The synergistic repair composition according to claim 1, characterized in that The weight percentage of the Artemia extract in the synergistic repair composition is 0.1%-2%.

5. The synergistic repair composition according to claim 1, characterized in that: The invention is composed of the following components in weight percentage: 0.01%-2% fibronectin, 0.001%-0.5% soluble proteoglycan, 0.1%-2% Artemia extract, and the balance is deionized water.

6. The synergistic repair composition according to claim 2, characterized in that: The weight percentage of the fibronectin in the synergistic repair composition is 0.025%-0.2%.

7. The synergistic repair composition according to claim 3, characterized in that: The weight percentage of the soluble proteoglycan in the synergistic repair composition is 0.00125%-0.00625%.

8. The synergistic repair composition according to claim 4, characterized in that The weight percentage of the Artemia extract in the synergistic repair composition is 0.125%-1%.

9. The method for preparing the synergistic repair composition according to any one of claims 1 to 8, characterized in that: The method comprises the following steps: mixing soluble proteoglycan with deionized water, stirring to dissolve, sequentially adding fibronectin and Artemia extract, stirring evenly, and obtaining a synergistic repair composition.

10. Use of the synergistic repair composition according to any one of claims 1 to 8 in repair cosmetics.

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