Pet oral soluble film and its preparation method

Abstract

The present invention discloses a pet orally disintegrating film and a preparation method thereof. The pet orally disintegrating film comprises a first film-forming agent, a pH regulator, a pet flavoring agent, a disintegrant and a supplement according to mass parts; the first film-forming agent is a wolfberry polysaccharide extract. In the pet orally disintegrating film of the present invention, the pH regulator adjusts the pH of the pet orally disintegrating film to match the pH of the saliva of pets (dogs and cats), and the disintegrant can cause the pet orally disintegrating film adhered to the corresponding part of the pet's (dog and cat's) oral cavity to quickly disintegrate, avoiding the discomfort of pets (dogs and cats) caused by long-term adhesion. The pet flavoring agent can improve the degree of pet's (dog and cat's) preference for the pet orally disintegrating film according to the taste characteristics of pets (dogs and cats). Compared with the prior art, the pet orally disintegrating film of the present invention is prepared according to the characteristics of pets (dogs and cats) to be suitable for pets (dogs and cats), is liked by pets (dogs and cats) during actual use, and can greatly avoid the discomfort of pets (dogs and cats) during taking.

Description

Technical Field

[0001] The present invention relates to the field of pet products, and in particular to a pet orally disintegrating film and a preparation method thereof. Background Art

[0002] There are two types of existing orally disintegrating film technologies. One is a product specifically for human medicines and nutritional supplements, and the other is the Korean orally disintegrating film for pets, which is a inactivated lactic acid bacteria supplement.

[0003] The existing orally disintegrating film technology has the basic preparation and molding dosage form suitable for human physiological structure and body characteristics. The existing Korean pet orally disintegrating film simply imitates the molding and preparation technology of human orally disintegrating film. Both preparation technologies do not prepare orally disintegrating films suitable for pets (dogs and cats) based on the characteristics of pets (dogs and cats). Summary of the Invention

[0004] Based on this, it is necessary to provide a pet orally disintegrating film that can solve the above problems.

[0005] In addition, it is also necessary to provide a method for preparing the above pet orally disintegrating film.

[0006] A pet orally disintegrating film, comprising, by weight, 20 to 50 parts of a first film-forming agent, 0.5 to 1.5 parts of a pH regulator, 3 to 10 parts of a pet flavoring agent, 0.5 to 5 parts of a disintegrant, and 10 to 20 parts of a supplement;

[0007] The first film-forming agent is wolfberry polysaccharide extract.

[0008] In one embodiment, the preparation method of the wolfberry polysaccharide extract is as follows: after the wolfberry is crushed, water, enzyme and vitamin C are added according to a material-liquid ratio of 0.5g~2g:10mL, and then mixed and enzymatically hydrolyzed for 0.5h~5h at a pH of 5.5~6.5 and a temperature of 50℃~65℃. After the reaction is completed, the liquid phase is separated and concentrated at a concentration density of 1.35~1.4 to obtain the wolfberry polysaccharide extract, wherein the amount of the enzyme is 0.25wt%~1wt%, the amount of the vitamin C is 0.2wt%~1wt%, and the enzyme includes cellulase and papain.

[0009] In one embodiment, in the enzyme, the ratio of the cellulase to the papain is 1 to 3:1.

[0010] In one embodiment, the pH of the pet orally disintegrating film is 8 to 10;

[0011] The pH regulator is selected from at least one of tetrasodium pyrophosphate, sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium carbonate, sodium bicarbonate, citric acid and lactic acid.

[0012] In one embodiment, the pet flavoring agent comprises monosaccharides or syrup, amino acids, vitamin B1, and yeast.

[0013] In one embodiment, the mass ratio of the monosaccharides or syrup, the amino acids, the vitamin B1, and the yeast is 10 - 20:1 - 1.9:0.2 - 1:1 - 3;

[0014] The monosaccharides are selected from at least one of xylose and ribose, the amino acids are selected from at least one of threonine, lysine, glycine, L-cysteine, and methionine, and the yeast is fermented beer yeast powder.

[0015] In one embodiment, the disintegrant is selected from at least one of sodium carboxymethyl starch, crospovidone, and croscarmellose sodium;

[0016] The supplement comprises tea polyphenols, theaflavins, mint, catnip, lysozyme, Compositae plant extract, B vitamins, and oral probiotics.

[0017] In one embodiment, the pet oral soluble film further comprises 20 parts - 50 parts of a second film-forming agent, and the second film-forming agent comprises modified starch, pullulan, and maltodextrin.

[0018] A method for preparing the above-mentioned pet oral soluble film comprises the following steps:

[0019] According to the mass parts, 3 parts - 10 parts of the pet flavoring agent and 20 parts - 50 parts of water are heated to 95°C - 100°C and kept warm for 25 min - 45 min, then cooled to 40°C - 55°C, and then 20 parts - 50 parts of the first film-forming agent are added. After mixing evenly, 0.5 parts - 1.5 parts of a pH regulator, 0.5 parts - 5 parts of the disintegrant, and 10 parts - 20 parts of the supplement are added. After mixing evenly again, film spreading, drying, demolding, cutting, and packaging are carried out in sequence to obtain the pet oral soluble film.

[0020] A method for preparing the above-mentioned pet oral soluble film comprises the following steps:

[0021] According to the mass parts, 20 parts - 50 parts of the first film-forming agent and 20 parts - 50 parts of the second film-forming agent are mixed and heated to 60°C - 80°C and stirred evenly to obtain a film-forming mixture;

[0022] According to parts by mass, 3 to 10 parts of a pet flavoring agent and 20 to 50 parts of water are heated to 95°C to 100°C and kept warm for 25 min to 45 min. Then, after cooling to 40°C to 55°C, the film-forming mixture is added. After mixing evenly, 0.5 to 1.5 parts of a pH regulator, 0.5 to 5 parts of a disintegrant, and 10 to 20 parts of a supplement are added. After mixing evenly again, film spreading, drying, demolding, cutting, and packaging are carried out in sequence to obtain the pet oral soluble film.

[0023] The pet oral soluble film of the present invention includes 20 to 50 parts of a first film-forming agent, 0.5 to 1.5 parts of a pH regulator, 3 to 10 parts of a pet flavoring agent, 0.5 to 5 parts of a disintegrant, and 10 to 20 parts of a supplement according to parts by mass. The pH regulator adjusts the pH of the pet oral soluble film to match the pH of the saliva of pets (dogs and cats). The disintegrant can cause the pet oral soluble film adhered to the corresponding parts of the pet's (dog and cat's) oral cavity to quickly disintegrate, avoiding discomfort caused by long-term adhesion to the pet (dog and cat). The pet flavoring agent can improve the pet's (dog and cat's) preference for the pet oral soluble film according to the taste characteristics of the pet (dog and cat).

[0024] Compared with the prior art, the pet oral soluble film of the present invention is prepared according to the characteristics of pets (dogs and cats) to obtain a pet oral soluble film suitable for pets (dogs and cats). It is liked by pets (dogs and cats) during actual use and can greatly avoid discomfort of pets (dogs and cats) during taking.

[0025] In addition, in the present invention, the first film-forming agent is wolfberry polysaccharide extract. The wolfberry polysaccharide extract specially extracted in the present invention not only has a good film-forming effect but also belongs to a natural plant polysaccharide with active ingredients. Its addition can avoid the addition of traditional film-forming agents and increase the proportion of active ingredients in the pet oral soluble film of the present invention. At the same time, the wolfberry polysaccharide extract also has the effects of anti-inflammatory, antibacterial, anti-stress, and enhancing immunity.

[0026] Furthermore, in combination with specific embodiments, the pet oral soluble film of the present invention also has the advantages of improving pet bad breath, reducing the growth of dental plaque, and having the effect of cleaning the oral cavity.

[0027] Preferably, in the present invention, the pet oral soluble film further includes 20 to 50 parts of a second film-forming agent, and the second film-forming agent includes modified starch, pullulan polysaccharide, and maltodextrin. Description of the Drawings

[0028] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0029] Wherein:

[0030] Figure 1 It is a flowchart of a preparation method of a pet orally dissolving film in an embodiment. Specific embodiments

[0031] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0032] It should be noted that all directional indications (such as up, down, left, right, front, back...) in the embodiments of the present invention are only used to explain the relative positional relationship and movement conditions between components in a specific posture. If the specific posture changes, the directional indications will also change accordingly.

[0033] In addition, the descriptions involving "first", "second", etc. in the present invention are only for descriptive purposes, and cannot be understood as indicating or implying their relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions between various embodiments can be combined with each other, but it must be based on the fact that those of ordinary skill in the art can implement it. When the combination of technical solutions conflicts with each other or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0034] The present invention discloses a pet orally dissolving film in an embodiment, which includes 20 to 50 parts of a first film-forming agent, 0.5 to 1.5 parts of a pH regulator, 3 to 10 parts of a pet flavoring agent, 0.5 to 5 parts of a disintegrant, and 10 to 20 parts of a supplement by mass.

[0035] Specifically, in the present invention, the first film-forming agent is wolfberry polysaccharide extract.

[0036] The pet oral soluble film of the present invention comprises, by mass parts, 20 to 50 parts of a first film-forming agent, 0.5 to 1.5 parts of a pH regulator, 3 to 10 parts of a pet flavoring agent, 0.5 to 5 parts of a disintegrant, and 10 to 20 parts of a supplement. The pH regulator adjusts the pH of the pet oral soluble film to match the pH of the saliva of pets (dogs and cats). The disintegrant can cause the pet oral soluble film adhered to the corresponding parts of the pet's (dog and cat's) oral cavity to disintegrate quickly, avoiding the discomfort of pets (dogs and cats) caused by long-term adhesion. The pet flavoring agent can improve the degree of pets' (dogs and cats') preference for the pet oral soluble film according to the taste characteristics of pets (dogs and cats).

[0037] Compared with the prior art, the pet oral soluble film of the present invention is prepared according to the characteristics of pets (dogs and cats) to produce a pet oral soluble film suitable for pets (dogs and cats). It is liked by pets (dogs and cats) during actual use and can greatly avoid the discomfort of pets (dogs and cats) during taking.

[0038] In addition, in the present invention, the first film-forming agent is wolfberry polysaccharide extract. The wolfberry polysaccharide extract specially obtained in the present invention not only has a good film-forming effect but also belongs to a natural plant polysaccharide with active ingredients. Its addition can avoid the addition of traditional film-forming agents and increase the proportion of active ingredients in the pet oral soluble film of the present invention. At the same time, wolfberry polysaccharide extract also has the effects of anti-inflammatory, antibacterial, anti-stress, and enhancing immunity.

[0039] Preferably, in this embodiment, the preparation method of wolfberry polysaccharide extract is as follows: After crushing wolfberries, water, enzymes, and vitamin C are added according to a material-liquid ratio of 0.5 g to 2 g: 10 mL, and then mixed and enzymatically hydrolyzed at a pH of 5.5 to 6.5 and a temperature of 50 °C to 65 °C for 0.5 h to 5 h. After the reaction is completed, the liquid phase is separated, and the liquid phase is concentrated at a concentration density of 1.35 to 1.4 to obtain wolfberry polysaccharide extract, wherein the dosage of the enzyme is 0.25 wt% to 1 wt%, the dosage of vitamin C is 0.2 wt% to 1 wt%, and the enzyme includes cellulase and papain.

[0040] By enzymatically hydrolyzing wolfberries with cellulase and papain, wolfberry polysaccharide in wolfberries can be released better. Wolfberry polysaccharide has viscosity, and by controlling the content of wolfberry polysaccharide, a good film-forming effect can be achieved. In addition, wolfberries also contain pectin components, and no pectinase is added during enzymatic hydrolysis, so that the obtained wolfberry polysaccharide extract contains pectin. Pectin has high film-forming properties, and the retention of pectin makes the film-forming property better.

[0041] More preferably, in the enzyme, the ratio of cellulase to papain is 1 to 3:1.

[0042] Specifically, the wolfberries can be fresh wolfberries or dried wolfberries.

[0043] When the sugar content of wolfberry is too low, the content of wolfberry polysaccharide is insufficient, and the film-forming property is poor or even unable to form a film; when the sugar content of wolfberry is too high, the content of wolfberry polysaccharide is too high, resulting in too high adhesiveness after film-forming and difficulty in demolding.

[0044] Preferably, the wolfberry is fresh wolfberry with a sugar content of 35% - 42% or dried wolfberry made from fresh wolfberry with a sugar content of 35% - 42%.

[0045] Preferably, in this embodiment, the pH of the pet oral soluble film is 8 - 10.

[0046] In the present invention, by detecting one hundred dogs and cats, it is statistically found that the pH of the saliva of dogs and cats is about 8 - 10. Therefore, the pH of the pet oral soluble film of the present invention will be adjusted to 8 - 10 by a pH regulator.

[0047] Specifically, in order to increase the compliance of oral feeding and improve the bioavailability of oral mucosa absorption, the scientifically and reasonably formulated pH regulator is selected from at least one of sodium pyrophosphate (sodium pyrophosphate), sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium carbonate, sodium bicarbonate, citric acid and lactic acid.

[0048] The pH regulator is selected from at least one of sodium pyrophosphate (sodium pyrophosphate), sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium carbonate, sodium bicarbonate, citric acid and lactic acid, which can make the pet oral soluble film be adjusted to a taste and smell preferred by dogs and cats, and the finished product with a similar oral pH value increases the compliance of dogs and cats.

[0049] The wolfberry polysaccharide extract is obtained by enzymatic hydrolysis, which preserves the original nutritional components of wolfberry. The enzymatic hydrolysis components not only increase the bioavailability of wolfberry itself, but also increase the bioavailability of other components.

[0050] In addition, in the production process, a certain heating temperature and stirring can well integrate the synergistic effects of various raw material components, thereby increasing the bioavailability of the efficacy raw materials.

[0051] Preferably, in this embodiment, the pet flavoring agent includes monosaccharide or syrup, amino acid, vitamin B1 and yeast.

[0052] The pet flavoring agent includes monosaccharide or syrup, amino acid, vitamin B1 and yeast. The Maillard reaction can occur between the monosaccharide or syrup, amino acid, vitamin B1 and yeast by strengthening the reaction between the raw materials in the production process, generating taste and smell and nutritional factors, so as to prepare an oral soluble film with good palatability for various oral nutritional supplements.

[0053] More preferably, in this embodiment, the mass ratio of monosaccharide or syrup, amino acid, vitamin B1 and yeast is 10 - 20:1 - 1.9:0.2 - 1:1 - 3.

[0054] Specifically, the monosaccharide can be selected from at least one of xylose and ribose, the amino acid can be selected from at least one of threonine, lysine, glycine, L-cysteine and methionine, and the yeast can be fermented beer yeast powder.

[0055] More preferably, in this embodiment, the amino acids include threonine, lysine, glycine, L-cysteine and methionine, and the mass ratio of threonine, lysine, glycine, L-cysteine and methionine is 0.2-0.4:0.3-0.5:0.2-0.6:0.2-0.6:0.1-0.5.

[0056] Particularly, in this embodiment, the amino acids include threonine, lysine and methionine, and the mass ratio of threonine, lysine and methionine is 0.2-0.4:0.3-0.5:0.5-1.

[0057] Preferably, in this embodiment, the disintegrant is selected from at least one of sodium carboxymethyl starch, cross-linked polyvinyl pyrrolidone and cross-linked sodium carboxymethyl cellulose.

[0058] In order to better improve the compliance of pets and at the same time play the corresponding functional role of active factors, it is necessary to scientifically formulate the speed and time of oral disintegration.

[0059] The optimal pH value promotes effective absorption of active factors by the oral mucosa. If the orally disintegrating film disintegrates too quickly, it will not be conducive to the retention of active functional factors in the oral cavity. If it disintegrates too slowly, it will cause dogs and cats to resist and feel disgusted with foreign objects in their mouths. Therefore, the disintegration time is controlled between 30s and 90s according to the functional factors.

[0060] Preferably, in this embodiment, the supplements include tea polyphenols, theaflavins, mint, catnip, lysozyme, Asteraceae plant extracts (chamomile, marigold, chrysanthemum), B vitamins and oral probiotics.

[0061] The pet orally dissolving film of this embodiment has the effects of refreshing the oral cavity, reducing the growth of dental plaque, cleaning the oral cavity, etc.

[0062] Tea polyphenols have the effects of preventing caries, strengthening teeth and eliminating bad breath.

[0063] Tea polyphenols can kill lactic acid bacteria and other caries-causing bacteria that live in the spaces between teeth. They inhibit the activity of glucose polymerase, preventing bacteria from taking root and effectively interrupting the process that causes caries. Protein residues in the spaces between teeth serve as a substrate for the proliferation of decay bacteria, which tea polyphenols can kill.

[0064] In addition, tea polyphenols can stimulate gastrointestinal reactions and accelerate large intestine peristalsis to achieve the effect of treating constipation. By enhancing the overall immune capacity of the human body, tea polyphenols can inhibit and kill various harmful pathogenic bacteria causing diarrhea and relieve the tense state of the gastrointestinal tract to achieve the effect of anti-inflammatory and antidiarrheal.

[0065] Theaflavins are a general term for a class of compounds with a benzotropolone structure, which are formed by the catalytic condensation of catechins by polyphenol oxidase during the fermentation process of black tea and are the main quality components of black tea.

[0066] Theaflavins have extremely strong physiological activities, including antioxidant, anti-inflammatory, antiviral, prevention and treatment of cardiovascular diseases, and antibacterial effects.

[0067] Mint and catnip have a refreshing taste, which can make the breath fresh and lasting, and can also prevent bad breath.

[0068] Erythritol is not utilized by Streptococcus mutans in the human oral cavity and can also inhibit oral pathogenic bacteria, so it can play a role in protecting teeth and has anti-caries properties.

[0069] Small-molecule erythritol not only cannot be utilized and decomposed by probiotics in the oral cavity, but can also inhibit oral pathogenic bacteria, thereby playing a role in protecting teeth and preventing caries.

[0070] Lysozyme is widely present in the animal kingdom, plant kingdom, and microbial kingdom, and is rich in lysozyme in the tears, saliva, milk, blood, body fluids, and internal organs of animals.

[0071] Lysozyme has the following advantages: (1) comprehensively improving the immune capacity of the animal body; (2) killing harmful bacteria, promoting the proliferation of beneficial bacteria, and improving the balance of the intestinal flora; (3) promoting the repair of damaged tissues and reducing inflammatory symptoms; (4) penetrating the reproductive barrier, cutting off vertical mother-offspring infection, and protecting both generations of mother and child; (5) preventing and controlling virus infections.

[0072] Wild chrysanthemum has the effects of clearing heat and detoxifying, dispelling wind and soothing the liver, etc., and is mainly used to treat erysipelas, eczema, and sore throat.

[0073] Honeysuckle is cold in nature and sweet in taste, and has the effects of cooling and dispersing wind-heat, clearing heat and detoxifying.

[0074] Honeysuckle contains chlorogenic acid, which has obvious curative effects on acute pharyngeal inflammation and skin diseases, and has strong inhibitory and killing effects on a variety of pathogenic bacteria such as Staphylococcus aureus, hemolytic streptococcus, Salmonella typhi, Shigella dysenteriae, and Streptococcus pneumoniae.

[0075] The combined use of wild chrysanthemum and honeysuckle has the effects of clearing heat and reducing fire, inhibiting bacteria and disinfecting, and treating acute tonsillitis, upper respiratory tract inflammation, and bacillary dysentery.

[0076] Marigold has anti-inflammatory, antioxidant, vision-protecting, immunity-boosting and other effects. Lutein is contained in honeysuckle, and lutein has biological functions such as enhancing the body's antioxidant capacity, reducing blood lipid levels, and improving the body's immune system function.

[0077] Studies have shown that when 300 g / kg of marigold extract is added to the diet of blue cats, the immunity of blue cats can be improved, and adding marigold extracts at different concentrations can enhance the body's antioxidant capacity.

[0078] In other embodiments, other types of supplements can also be used, which can play other roles.

[0079] Preferably, in this embodiment, the pet oral soluble film further comprises 20 to 50 parts of a second film-forming agent, and the second film-forming agent includes modified starch, pullulan and maltodextrin.

[0080] Specifically, the modified starch is selected from at least one of acetate starch, oxidized starch and hydroxypropyl starch.

[0081] The modified starch also has a disintegrating effect, and the increase of the modified starch is also beneficial to the disintegration of the pet oral soluble film in the oral cavity.

[0082] Furthermore, in combination with specific embodiments, the pet oral soluble film of the present invention also has the advantages of improving pet bad breath, reducing the growth of dental plaque, and having the effect of cleaning the oral cavity.

[0083] The present invention also discloses a preparation method of the above-mentioned pet oral soluble film in an embodiment, comprising the following steps:

[0084] According to the mass parts, 3 to 10 parts of a pet flavoring agent and 20 to 50 parts of water are heated to 95°C to 100°C and kept warm for 25 min to 45 min, then cooled to 40°C to 55°C, and then 20 to 50 parts of a first film-forming agent are added. After mixing evenly, 0.5 to 1.5 parts of a pH regulator, 0.5 to 5 parts of a disintegrant and 10 to 20 parts of a supplement are added. After mixing evenly again, film spreading, drying, demoulding, cutting and packaging are carried out in sequence to obtain the pet oral soluble film.

[0085] Preferably, the pet flavoring agent includes monosaccharide or syrup, amino acid, vitamin B1 and yeast. The pet flavoring agent and water are heated to 95°C to 100°C and kept warm for 25 min to 45 min, so that the monosaccharide or syrup, amino acid, vitamin B1 and yeast undergo the Maillard reaction to produce a pleasant smell and nutritional factors, thereby preparing an oral soluble film with good palatability for various oral nutritional supplements.

[0086] Combined Figure 1 , the present invention also discloses another preparation method of the above-mentioned pet oral soluble film in an embodiment, comprising the following steps:

[0087] S10. According to the parts by mass, mix 20 to 50 parts of the first film-forming agent and 20 to 50 parts of the second film-forming agent, then heat the mixture to 60°C to 80°C and stir evenly to obtain a film-forming mixture.

[0088] After the first film-forming agent and the second film-forming agent are mixed, heated to 60°C to 80°C and stirred evenly, the obtained film-forming mixture is a basic film efficacy mixture.

[0089] S20. According to the parts by mass, heat 3 to 10 parts of a pet flavoring agent and 20 to 50 parts of water to 95°C to 100°C and keep warm for 25 min to 45 min. Then cool down to 40°C to 55°C and add the film-forming mixture. After mixing evenly, add 0.5 to 1.5 parts of a pH regulator, 0.5 to 5 parts of a disintegrant, and 10 to 20 parts of a supplement. After mixing evenly again, perform film spreading, drying, demolding, cutting, and packaging in sequence to obtain the pet oral soluble film.

[0090] Preferably, the pet flavoring agent includes monosaccharides or syrups, amino acids, vitamin B1, and yeast. The pet flavoring agent and water are heated to 95°C to 100°C and kept warm for 25 min to 45 min, enabling the monosaccharides or syrups, amino acids, vitamin B1, and yeast to undergo the Maillard reaction, generating flavors and nutritional factors, thereby preparing an oral soluble film with good palatability for various oral nutritional supplements.

[0091] The following are specific examples.

[0092] In the specific examples, the wolfberry is fresh Ningxia wolfberry with a sugar content of 35%. The cellulase, papain, and lysozyme are all purchased from Novozymes, and the oral ADP-1 probiotic is purchased from Jingyue Biotechnology Co., Ltd.

[0093] Example 1

[0094] After crushing 100 g of wolfberry, add 1000 mL of water, 5 g of vitamin C, and 5 g of enzymes. Then, under the conditions of pH 6 and a temperature of 55°C, perform enzymatic hydrolysis for 3 h. After the reaction is completed, separate the liquid phase and concentrate the liquid phase at a concentration density of 1.35 to 1.4 to obtain wolfberry polysaccharide extract, where the enzymes are cellulase and papain in a ratio of 2:1.

[0095] Mix 5 g of pet flavoring agent (xylose, threonine, lysine, glycine, L-cysteine, methionine and fermented beer yeast powder with a mass ratio of 15:0.3:0.4:0.8:0.6:2) and 30 g of water, heat the mixture to 100 °C, keep it warm for 35 minutes, cool the reaction solution to 50 °C, add 30 g of the prepared wolfberry polysaccharide extract obtained above, stir and mix evenly, then add 1.5 g of sodium pyrophosphate, 0.2 g of citric acid, 1 g of sodium carboxymethyl starch, 5.5 g of Compositae plant extract, 6 g of lysozyme and 5 g of tea polyphenols, stir and mix evenly for 20 minutes, add 3 g of B vitamins, 2 g of catnip, 1 g of oral ADP-1 probiotic and stir and mix for 5 minutes. Finally, spread the film, dry it, demold it, cut it and package it to obtain the required pet oral soluble film.

[0096] Example 2

[0097] Crush 100 g of wolfberries, add 1000 mL of water, 5 g of vitamin C and 5 g of enzymes, then mix and enzymatically hydrolyze for 3 h under the conditions of pH 6 and temperature 55 °C. After the reaction is completed, separate the liquid phase and concentrate the liquid phase at a concentration density of 1.35 - 1.4 to obtain wolfberry polysaccharide extract. Among them, the enzyme is a cellulase and papain with a ratio of 2:1.

[0098] Mix 30 g of the prepared wolfberry polysaccharide extract obtained above, 22.5 g of acetate starch, 15 g of pullulan polysaccharide and 10 g of maltodextrin, heat the mixture to 70 °C and stir for 40 min to obtain a film-forming mixture.

[0099] Mix 5 g of pet flavoring agent (xylose, threonine, lysine, glycine, L-cysteine, methionine and fermented beer yeast powder with a mass ratio of 15:0.3:0.4:0.8:0.6:2) and 30 g of water, heat the mixture to 100 °C, keep it warm for 35 minutes, cool the reaction solution to 50 °C, add the prepared film-forming mixture obtained above, stir and mix evenly, then add 1.5 g of sodium pyrophosphate, 0.2 g of citric acid, 1 g of sodium carboxymethyl starch, 5.5 g of Compositae plant extract, 6 g of lysozyme and 5 g of tea polyphenols, stir and mix evenly for 20 minutes, add 3 g of B vitamins, 2 g of catnip, 1 g of oral ADP-1 probiotic and stir and mix for 5 minutes. Finally, spread the film, dry it, demold it, cut it and package it to obtain the required pet oral soluble film.

[0100] Comparative Example 1

[0101] Mix 5 g of pet flavoring agent (xylose, threonine, lysine, methionine, and fermented brewer's yeast powder with a mass ratio of 15:0.3:0.4:0.8:2) and 30 g of water, heat the mixture to 100 °C, keep it warm for 35 minutes, cool the reaction solution to 50 °C, add 30 g of wolfberry extract containing wolfberry polysaccharide (Ningxia wolfberry extract purchased from Shaanxi Yinuo Biotechnology Co., Ltd., product number 009), stir and mix evenly, then add 1.5 g of sodium pyrophosphate, 0.2 g of citric acid, 1 g of sodium carboxymethyl starch, 5.5 g of Compositae plant extract, 6 g of lysozyme, and 5 g of tea polyphenols, stir and mix evenly for 20 minutes, add 3 g of B vitamins, 2 g of catnip, and 1 g of oral ADP-1 probiotic, stir and mix for 5 minutes, and finally spread the film, dry it, demold it, cut it, and package it to obtain the required pet oral soluble film.

[0102] Test Example

[0103] 1) Compare the film-forming properties of the pet oral soluble films prepared in Example 1 and the comparative example respectively.

[0104] The test results are as follows:

[0105] The mixed solution of Example 1 can be spread into a film without abnormality, can form a smooth and uniform thin film, and the demolding is smooth without deformation.

[0106] During the film spreading process of the mixed solution of the comparative example, holes of different sizes appear and a complete film sheet cannot be formed.

[0107] It can be seen from this that the wolfberry polysaccharide extract used in Example 1 has better film-forming properties than the commercially available wolfberry polysaccharide. 9]

[0108] 2) Feeding test of the pet oral soluble film prepared in Example 2 and the commercially available pet oral soluble film.

[0109] Select 50 dogs with oral problems, aged 1 to 12 years old, weighing 5 kg to 40 kg, and 50 cats with oral problems, aged 1.5 to 10 years old, weighing 4 to 8 kg. Divide them into two groups and feed them the pet oral soluble film prepared in Example 2 and the commercially available pet oral soluble film respectively. The feeding frequency is once a day, 150 mg each time.

[0110] Among them, the clinical manifestations of cats / dogs with oral problems are dysphagia, bad breath, salivation, weight loss, strong oral discomfort, oral bleeding, dull coat, and dyspnea.

[0111] After continuous feeding for one week, the test results are shown in Table 1 below.

[0112]

[0113] The above-described embodiments merely represent several implementation manners of the present invention. The description is relatively specific and detailed, but it should not be construed as a limitation on the scope of the patent application. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can still be made, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent shall be subject to the appended claims.

Claims

1. A preparation method of a pet orally disintegrating film, characterized in that It includes the following steps: According to the mass parts, heat 3 to 10 parts of pet flavoring agent and 20 to 50 parts of water to 95°C to 100°C and keep warm for 25 min to 45 min. Then, cool down to 40°C to 55°C and add 20 to 50 parts of the first film-forming agent. After mixing evenly, add 0.5 to 1.5 parts of pH regulator, 0.5 to 5 parts of disintegrant, and 10 to 20 parts of supplement. Mix evenly again and then carry out film spreading, drying, demolding, cutting, and packaging in sequence to obtain the pet oral soluble film. The first film-forming agent is wolfberry polysaccharide extract; The preparation method of the wolfberry polysaccharide extract is as follows: Crush wolfberries and add water, enzyme, and vitamin C according to the material-liquid ratio of 0.5 g to 2 g: 10 mL. Then, carry out mixed enzymatic hydrolysis for 0.5 h to 5 h under the conditions of pH 5.5 to 6.5 and temperature 50°C to 65°C. After the reaction is completed, separate the liquid phase and concentrate the liquid phase with a concentration density of 1.35 to 1.4 to obtain the wolfberry polysaccharide extract. Among them, the dosage of the enzyme is 0.25 wt% to 1 wt%, the dosage of vitamin C is 0.2 wt% to 1 wt%, and the enzyme is cellulase and papain.

2. The preparation method of the pet orally dissolving film according to claim 1, wherein, In the enzyme, the ratio of cellulase to papain is 1 to 3:

1.

3. The preparation method of the pet orally disintegrating film according to claim 1, characterized in that, The pH of the pet oral soluble film is 8 to 10; The pH regulator is selected from at least one of sodium pyrophosphate, sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium carbonate, sodium bicarbonate, citric acid, and lactic acid.

4. The preparation method of the pet orally dissolving film according to claim 1, wherein, The pet flavoring agent includes monosaccharide or syrup, amino acid, vitamin B1, and yeast.

5. The preparation method of the pet orally disintegrating film according to claim 4, characterized in that, The mass ratio of the monosaccharide or syrup, the amino acid, the vitamin B1, and the yeast is 10 to 20: 1 to 1.9: 0.2 to 1: 1 to 3; The monosaccharide is selected from at least one of xylose and ribose, the amino acid is selected from at least one of threonine, lysine, glycine, L-cysteine, and methionine, and the yeast is fermented beer yeast powder.

6. The preparation method of the pet orally disintegrating film according to claim 1, characterized in that, The disintegrant is selected from at least one of sodium carboxymethyl starch, cross-linked polyvinylpyrrolidone, and cross-linked carboxymethylcellulose sodium; The supplement includes tea polyphenols, theaflavins, mint, catnip, lysozyme, asteraceae plant extract, B vitamins, and oral probiotics.

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