Product capable of relieving constipation of autistic children and preparation method thereof

Abstract

The invention provides a product capable of relieving constipation of autistic children and a preparation method of the product, and belongs to the technical field of microorganisms and the technical field of medicines. The product capable of relieving constipation of autistic children comprises the following components in percentage by weight: 20-30% of inulin, 12-18% of galactooligosaccharide, 8-12% of fructo-oligosaccharide, 8-12% of stachyose, 8-12% of dietary fibers, 8-12% of hawthorn fruit powder, 8-12% of lactobacillus plantarum N-1 bacterial powder, 3-7% of Chinese yam powder and 3-7% of resistant dextrin. The product can improve the microbial environment of brain and intestinal tracts, can well relieve autism and regulate gastrointestinal tract problems of children with autism, especially constipation, and can effectively relieve mood, improve sleep state and self-cognition. Therefore, the product containing the lactobacillus plantarum N-1 bacterial powder has a great application prospect in relieving constipation of autistic children.

Description

Technical Field

[0001] The present invention relates to a biological product and a preparation method thereof, in particular to a product capable of relieving constipation in children with autism and a preparation method thereof, belonging to the fields of microbial technology and medical technology. Background Art

[0002] Autism, also known as autism, is a behavioral disorder characterized by lack of social interaction, language communication and interest, repetitive stereotyped behaviors or movements, and compulsion to maintain the current living environment and lifestyle. It is classified as a developmental disorder caused by a disorder of the nervous system. However, the cause of autism is not yet fully understood, and there is a lack of effective treatment and intervention methods.

[0003] Autism is a biological disease, which is influenced by both genetic and environmental factors, and the environment may be the main influencing factor. Abnormal early brain development in children with autism may be a direct cause of autism. Neurotoxic substances, nutrients, metabolites and neuroactive substances may all be causes of autism. Some autistic patients have chromosomal abnormalities and gene mutations. At the same time, more cases also show abnormalities in other aspects such as gastrointestinal symptoms (such as constipation, diarrhea, abdominal pain and gastroesophageal reflux). The higher proportion of abnormal gastrointestinal symptoms in autistic patients is closely related to the disorder of intestinal microorganisms in the patients. Therefore, improving the function of the bacteria-gut-brain axis composed of microorganisms, intestines and brain can alleviate the gastrointestinal problems of autistic children.

[0004] At present, there are a variety of intervention methods for different influencing factors, including biological and non-biological therapies. Biological therapies include antibiotics, drugs, and diet. Among them, autism therapy targeting intestinal microorganisms for detection and intervention will be the trend of the future, and intervention with probiotics and fecal microbiota transplantation is also attracting more and more attention. Although these therapies are used, they cannot effectively improve the symptoms of constipation in children with autism.

[0005] The prior art CN108728382A discloses: the use of Lactobacillus plantarum N-1 in lowering cholesterol and promoting the production of short-chain fatty acids in the intestine, and the use of Lactobacillus plantarum N-1 in preparing functional foods with the ability to lower cholesterol and promote the production of short-chain fatty acids in the intestine; CN112402465A discloses: a Lactobacillus plantarum N-1 in preparing products (microecological preparations, foods) for preventing and / or improving benign prostatic hyperplasia and uses (preventing and / or improving benign prostatic hyperplasia, preventing bladder outlet obstruction in the early stages of benign prostatic hyperplasia). Also, although, “Lactiplantibacillusplantarum N-1 improves autism-like behavior and gut microbiota in mouse, QiuZhongqing;Luo Dongmei;Yin Heng;Chen Yajun;ZhouZhiwei;Zhang Jing;Zhang Linzhu;Xia Jinrong;Xie Jiang;Sun Qun;Xu Wenming, Frontiers in Microbiology, 2023-03-16” disclosed: Lactobacillus plantarum-1 intervention can rescue autism-like behaviors in mice, including anxiety and depression; and, autism-like behaviors may be improved by regulating the intestinal microbiota, but it only records the theoretical basis, but combined with reality, there is no corresponding product to relieve constipation in autistic children, that is, no corresponding industrial application and adaptive production have been carried out. Summary of the invention

[0006] In order to overcome the shortcomings of the prior art, the present invention proposes a product that can relieve constipation in children with autism and a preparation method thereof. This technical solution not only obtains a product that can relieve constipation in children with autism through the coordination of specific components and an adaptive preparation process, but also ensures the performance stability of the product and can achieve industrial production (such as: easy to obtain raw materials, low cost, appropriate matching; stable preparation process, strong controllability, etc.) to meet actual needs.

[0007] In order to achieve the above technical objectives, the following technical solutions are proposed: The first purpose of the technical scheme is to propose: a product that can relieve constipation in children with autism, comprising the following components by weight percentage: 20-30% inulin, 12-18% galacto-oligosaccharide, 8-12% fructo-oligosaccharide, 8-12% stachyose, 8-12% dietary fiber, 8-12% hawthorn fruit powder, 8-12% plant lactobacillus N-1 powder, 3-7% yam powder and 3-7% resistant dextrin; In the Lactobacillus plantarum N-1 powder, the Lactobacillus plantarum N-1 preservation number is: CGMCC No.15463; the preservation time is: March 20, 2018; the preservation unit is: Institute of Microbiology, Chinese Academy of Sciences.

[0008] Preferably, the product comprises the following components by weight percentage: 25% inulin, 15% galacto-oligosaccharide, 10% fructo-oligosaccharide, 10% stachyose, 10% dietary fiber, 10% hawthorn fruit powder, 10% Lactobacillus plantarum N-1 powder, 5% yam powder and 5% resistant dextrin.

[0009] Preferably, the dietary fiber is one or a mixture of two or more of potato dietary fiber, oat dietary fiber and konjac dietary fiber.

[0010] Preferably, the number of viable bacteria in the Lactobacillus plantarum N-1 powder is 5.0*10 10 ~2.0*10 12 CFU / g.

[0011] Preferably, the preparation is formulated into gelatin-coated tablets, pellets, microcapsules, capsules or tablets.

[0012] The second purpose of this technical solution is to propose: a method for preparing a product that can relieve constipation in children with autism, comprising: A. Weigh inulin, galacto-oligosaccharide, fructo-oligosaccharide, stachyose and dietary fiber according to the proportion, add sterile water to dissolve, and obtain solution A; B. Weigh hawthorn fruit powder and yam powder according to the ratio, add sterile water to dissolve, and obtain solution B; C. Weigh Lactobacillus plantarum N-1 powder and resistant dextrin respectively according to the ratio, mix the dissolving solution A, the dissolving solution B, the Lactobacillus plantarum N-1 powder and the resistant dextrin evenly, and control the mixing time to be ≥30min to obtain the dissolving solution C; D. Centrifuge the dissolved solution C and remove the supernatant; then, freeze-dry and control the moisture content of the product to ≤3.0% to obtain the product; store at 0-4°C.

[0013] During the preparation process, control the following: the temperature of the production line ≤ 25°C, the relative humidity of the air ≤ 50%, to ensure that the preparation process is stable and highly controllable.

[0014] The third purpose of this technical solution is to propose: a method for using a product that can relieve constipation in children with autism, comprising: The product is dissolved in water at 30-45°C at a ratio of product weight (g) to water volume (mL) of 1:10-15, and stirred until completely dissolved; then, it is directly administered orally.

[0015] The beneficial technical effects brought about by adopting this technical solution are: 1. The present invention provides a product that can relieve constipation in children with autism. The product is composed of Lactobacillus plantarum N-1 powder, dietary fiber and a variety of nutrients. The product can regulate the function of the balanced bacteria-intestine-brain axis, that is, the product can improve the microbial environment of the brain and intestines, can effectively relieve autism, regulate gastrointestinal problems of children with autism, especially constipation, and can effectively relieve emotions, improve sleep status and self-cognition. Therefore, products including Lactobacillus plantarum N-1 powder have great application prospects in alleviating the problem of constipation in children with autism; 2. The plant lactobacillus N-1 involved in the present invention was first recorded in the inventor's previous research (CN108728382A) and was deposited in the General Microbiology Center of China Microorganism Culture Collection Committee (referred to as CGMCC, address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Postal Code: 100101) on March 20, 2018, with a deposit number of CGMCC NO. 15463. The plant lactobacillus N-1 was isolated from the traditional yak milk cheese products of Kalong Village, Mula Township, Daocheng County, Ganzi Tibetan Autonomous Prefecture, Sichuan Province. Plant lactobacillus N-1 is combined with a variety of nutritional elements and auxiliary materials to ensure the content of live bacteria on the one hand, which can effectively relieve the symptoms of constipation in children with autism; on the other hand, it ensures that it can be well dissolved in water when eaten to form a uniform solution, and prevent the formation of flocs, stratification or colloid, and has no peculiar smell, which is convenient for children with autism to eat; 3. The product for relieving constipation in autistic children involved in the present invention can be industrially produced (such as: the raw materials are easily available, the cost is low, and the combination is appropriate; the preparation process is stable and highly controllable, etc.) to meet actual needs. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 A product diagram of the product involved in the present invention; Figure 2 This is a diagram of the dissolution state of the product involved in the present invention (A is the dissolution state of the product just put into water, B is the dissolution state during stirring, and C is the state after complete dissolution); Figure 3 Statistical graph of the number of viable Lactobacillus plantarum N-1 in products stored at different temperatures; Figure 4 Statistical line graph of the detection of viable bacteria count of Lactobacillus plantarum N-1 in products stored at different temperatures. DETAILED DESCRIPTION

[0017] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0018] In the following embodiments, the plant lactobacillus N-1 powder involved has a preservation number of CGMCC No. 15463; a preservation date of March 20, 2018; a preservation unit of the Institute of Microbiology, Chinese Academy of Sciences; and a live cell count of 5.0*10 10 ~2.0*10 12 CFU / g; the product is formulated into gelatin-coated tablets, pellets, microcapsules, capsules or tablets.

[0019] Example 1 The present embodiment provides: a product that can relieve constipation in children with autism, comprising the following components, measured by weight percentage: 20% inulin, 18% galacto-oligosaccharide, 8% fructo-oligosaccharide, 8% stachyose, 8% potato dietary fiber, 12% hawthorn fruit powder, 12% plant lactobacillus N-1 powder, 7% yam powder and 7% resistant dextrin.

[0020] The product is in powder form with a milky aroma. It does not separate into layers after dissolving, forming a uniform solution. The number of live bacteria in the Lactobacillus plantarum N-1 powder is 5.0*10 10 ~2.0*10 12 CFU / g.

[0021] Example 2 The present embodiment provides: a product that can relieve constipation in children with autism, comprising the following components, measured in weight percentage: 30% inulin, 12% galacto-oligosaccharide, 12% fructo-oligosaccharide, 8% stachyose, 12% konjac dietary fiber, 8% hawthorn fruit powder, 8% plant lactobacillus N-1 powder, 3% yam powder and 7% resistant dextrin.

[0022] The product is in powder form with a fruity aroma. It does not separate into layers after dissolving, forming a uniform solution. The number of live bacteria in the Lactobacillus plantarum N-1 powder is 5.0*10 10 ~2.0*10 12 CFU / g.

[0023] Example 3 The present embodiment provides: a product that can relieve constipation in children with autism, comprising the following components, measured by weight percentage: 21% inulin, 17% galacto-oligosaccharide, 11% fructo-oligosaccharide, 10% stachyose, 10% oat dietary fiber, 11% hawthorn fruit powder, 11% plant lactobacillus N-1 powder, 6% yam powder and 3% resistant dextrin.

[0024] The product is in powder form with a milky aroma. It does not separate into layers after dissolving, forming a uniform solution. The number of live bacteria in the Lactobacillus plantarum N-1 powder is 5.0*10 10 ~2.0*10 12 CFU / g.

[0025] Example 4 The present embodiment provides: a product that can relieve constipation in children with autism, comprising the following components, measured by weight percentage: 25% inulin, 15% galacto-oligosaccharide, 10% fructo-oligosaccharide, 10% stachyose, 10% potato dietary fiber, 10% hawthorn fruit powder, 10% plant lactobacillus N-1 powder, 5% yam powder and 5% resistant dextrin.

[0026] The product is in powder form with a milky aroma. It does not separate into layers after dissolving, forming a uniform solution. The number of live bacteria in the Lactobacillus plantarum N-1 powder is 5.0*10 10 ~2.0*10 12 CFU / g.

[0027] Example 5 For the Lactobacillus plantarum N-1 powder in Examples 1-4, the separation, screening and molecular biological identification of the Lactobacillus plantarum CGMCC 15463 involved were carried out according to the inventor's previous research (CN108728382A), as follows: 1. Material preparation Milk cake samples were provided by Kalong Village, Mula Township, Daocheng County, Sichuan Province; The universal primer pair was synthesized by Invitrogen (Shanghai), and the sequence of 27F / 1492R is as follows: 27F: AGAGTTTGATCMTGGCTCAG 1492R:TACGGYTACCTTGTTACGACTT MRS liquid culture medium: 20.0 g glucose, 10.0 g peptone, 10.0 g beef extract, 6.0 g yeast powder, 1.0 mL Tween 80, 2.0 g potassium dihydrogen phosphate, 2.0 g ammonium citrate, 5.0 g sodium acetate, 0.58 g magnesium sulfate, 0.25 g manganese sulfate, 1 L pure water, natural pH (add 1.5%-2.0% agar for solid culture medium).

[0028] 2. Extraction process 10.0 g of traditional yak cheese (milk cake) sample was weighed and fully stirred in a conical flask containing 90 mL of sterile water, and then placed on a shaker for 60 min to completely disperse the sample. The suspension was aspirated, 10-fold gradient dilution was spread on MRS solid medium, and cultured at 37℃ for 48 h. Single colonies of different morphology and size were picked by naked eye observation, and the plate was streaked for purification three times. The purified strain was stored in 50% glycerol and frozen at -40℃ for later use. After the purified strain was proliferated and cultured in the corresponding liquid culture medium, the strain DNA was extracted using the Tiangen Bacterial Genomic DNA Extraction Kit, and the bacterial universal primers 27F / 1492R were used to amplify the 16S rRNA. The measured 16S rRNA sequence was compared with NCBI BLAST, and the similarity rate with the Lactobacillus plantarum standard strain MH19 in Genebank was 100%. The strain was preliminarily identified as a Lactobacillus plantarum, i.e., Lactobacillus plantarum.

[0029] Lactobacillus plantarum CGMCC 15463 was streaked on MRS agar medium and inverted cultured at 37°C for 48 h, and then the colony morphology of the strain was observed.

[0030] The strain grows well on MRS agar medium, and the colonies are milky white, smooth in surface, neat in edge, opaque, and catalyst negative; the bacterial morphology of the plant lactobacillus CGMCC 15463 is examined under a microscope, and Gram staining shows purple and rod-shaped.

[0031] Example 6 Based on Examples 1-4, this example provides: a method for using a product that can relieve constipation in autistic children, comprising: dissolving the product in 30-45°C water at a ratio of product weight (g) to water volume (mL) of 1:10-15, stirring until completely dissolved (e.g. Figure 2 Then, directly administer orally.

[0032] Alternatively, you can eat it dry or mix it with milk, juice, etc.

[0033] Example 7 Based on Examples 1-5, this example provides: a method for preparing a product that can relieve constipation in children with autism, comprising: A. Weigh inulin, galacto-oligosaccharide, fructo-oligosaccharide, stachyose and dietary fiber according to the proportion, add sterile water to dissolve, and obtain solution A; B. Weigh hawthorn fruit powder and yam powder according to the ratio, add sterile water to dissolve, and obtain solution B; C. Weigh the Lactobacillus plantarum N-1 powder and the resistant dextrin respectively according to the ratio, and evenly mix the dissolving solution A, the dissolving solution B, the Lactobacillus plantarum N-1 powder and the resistant dextrin to obtain the dissolving solution C; D. Centrifuge the dissolved solution C and remove the supernatant; then, freeze-dry to obtain the product; store at 0-4°C. Figure 1 shown.

[0034] In addition, this example also discusses the viable count of Lactobacillus plantarum N-1 in the product at different storage temperatures. The results are shown in Tables 1 and Figure 3-4 shown.

[0035] Combining Table 1 and Figure 3-4 It can be seen that: with the increase of storage time, the number of viable bacteria of Lactobacillus plantarum N-1 in the 4℃ refrigerated environment showed a slow decrease trend, and the number of viable bacteria after 240 days was still around 10 10 At room temperature of 15-25℃, the number of viable bacteria of Lactobacillus plantarum N-1 increased from 10 10 Down to 10 7 .

[0036] In addition, after the preparation of the plant lactobacillus N-1 powder involved, according to the requirements of the batching process, the weight of the components in the product formula are mixed in equal increments from small to large, and the dilution mixing ratio is controlled so as not to exceed 1:10. When the total amount of the mixed material is ≤8kg, the required raw materials are mixed in equal increments to ensure that the mixing time is ≥30 minutes; when the total amount of the material is ≥8kg, the required raw materials are first mixed in equal increments, and then re-mixed using an automatic mixer. The total amount of a single mixed material should be controlled within 60kg, and the mixing time should be ≥30 minutes.

[0037] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Any simple modification or equivalent change made to the above embodiment based on the technical essence of the present invention shall fall within the protection scope of the present invention.

Claims

1. A product for relieving constipation in children with autism, characterized in that: The composition comprises the following components by weight percentage: 20-30% inulin, 12-18% galacto-oligosaccharide, 8-12% fructo-oligosaccharide, 8-12% stachyose, 8-12% dietary fiber, 8-12% hawthorn fruit powder, 8-12% plant lactobacillus N-1 powder, 3-7% yam powder and 3-7% resistant dextrin; Among them, the preservation number of Lactobacillus plantarum N-1 is CGMCC No.15463, the preservation time is March 20, 2018, and the preservation unit is the Institute of Microbiology, Chinese Academy of Sciences.

2. The product for relieving constipation in children with autism according to claim 1, characterized in that: The product comprises the following components by weight percentage: 25% inulin, 15% galacto-oligosaccharide, 10% fructo-oligosaccharide, 10% stachyose, 10% dietary fiber, 10% hawthorn fruit powder, 10% plant lactobacillus N-1 powder, 5% yam powder and 5% resistant dextrin.

3. The product for relieving constipation in children with autism according to claim 2, characterized in that: The dietary fiber is one of potato dietary fiber, oat dietary fiber and konjac dietary fiber, or a mixture of two or more thereof.

4. The product for relieving constipation in children with autism according to claim 1, characterized in that: The number of live bacteria in the Lactobacillus plantarum N-1 powder is 5.0*10 10 ~2.0*10 12 CFU / g.

5. The product for relieving constipation in children with autism according to claim 1, characterized in that: The method for using the product comprises: dissolving the product in water at 30 to 45°C at a ratio of product weight (g) to water volume (mL) of 1:10 to 15, stirring until completely dissolved; and then directly administering the product orally.

6. The method for preparing the product capable of relieving constipation in autistic children according to any one of claims 1 to 5, characterized in that: The steps include: A. Weigh inulin, galacto-oligosaccharide, fructo-oligosaccharide, stachyose and dietary fiber according to the proportion, add sterile water to dissolve, and obtain solution A; B. Weigh hawthorn fruit powder and yam powder according to the ratio, add sterile water to dissolve, and obtain solution B; C. Weigh the Lactobacillus plantarum N-1 powder and the resistant dextrin respectively according to the ratio, and evenly mix the dissolving solution A, the dissolving solution B, the Lactobacillus plantarum N-1 powder and the resistant dextrin to obtain the dissolving solution C; D. Centrifuge the solution C and remove the supernatant; Then, freeze-drying is performed to obtain the product, which is stored at 0-4°C.

7. The method for preparing the product capable of relieving constipation in autistic children according to claim 6, characterized in that: In step C, the mixing time is controlled to be ≥30 min.

8. The method for preparing the product capable of relieving constipation in children with autism according to claim 7, characterized in that: In the freeze-drying of step D, the moisture content of the product is controlled to be ≤3.0%.

9. The method for preparing the product capable of relieving constipation in autistic children according to claim 8, characterized in that: Control: The temperature of the production line is ≤25℃, and the relative humidity of the air is ≤50%.

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