An acidified soil conditioner and method of manufacture

Abstract

The application relates to an acidified soil conditioner and a preparation method, and belongs to the technical field of soil conditioners. The acidified soil conditioner is prepared from the following raw materials in parts by weight: bacillus subtilis 10-15 parts, EM bacteria 10-15 parts, ethylhexyl glycerin 20-30 parts, chitin 3-8 parts, sodium tripolyphosphate 10-15 parts, calcium-magnesium phosphate fertilizer 25-35 parts, allantoin 12-15 parts, modified oyster shell 20-30 parts and modified beer lees 35-45 parts. The soil conditioner can improve soil acidity, increase the pH value, increase the content of organic matter and other nutrient components, reduce the content of heavy metals, is beneficial to crop growth, and improves the quality and yield of crops.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of soil conditioners, and relates to an acidified soil conditioner and a preparation method. BACKGROUND

[0002] Soil acidification refers to the process in which a soil cation exchange complex accepts a certain amount of exchangeable hydrogen ions or aluminum ions, resulting in the leaching of basic (base) ions in the soil.

[0003] 1. Large and concentrated rainfall and strong leaching result in the loss of a large amount of calcium, magnesium, potassium and other basic salts, which is the fundamental cause of soil acidification. 2. The lack of traditional agricultural measures such as applying lime, burning dung and applying organic fertilizer causes the imbalance of nutrients in cultivated soil, which is the main cause of soil acidification. 3. Long-term and large-scale application of chemical fertilizers is an important cause of soil acidification, and even long-term application of urea also causes soil acidification.

[0004] The pH value of soil is crucial for plant growth. Most crops grow well in neutral soil with a pH value of 7 or slightly acidic soil. Once the pH value of the soil decreases, diseases and pests will hinder the growth of plants, such as the growth of plant roots, affect the absorption of nutrients by plants, and lead to a decrease in crop yield and quality; meanwhile, soil acidification will increase the toxic effect of soil metals, and a strong acid environment will accelerate the filtration of toxic metals into surrounding water bodies.

[0005] Applying lime is the main measure for improving acid soil in current agricultural production, but the lime improvement effect is poor in persistence, and improper application can easily lead to soil compaction and nutrient imbalance. The main lime-based improvers on the market can be divided into four categories: carbonates, oxides, hydroxides and by-products. According to their neutralization ability from strong to weak, it is oxide ≥ hydroxide > carbonate ≥ lime by-product. Although pure oxides and hydroxides can quickly increase the pH value of soil, they have obvious side effects, and they will absorb moisture and generate high temperature in the process of neutralizing acid, which will affect crops. In addition, excessive application will reduce soil organic matter and cause soil compaction. SUMMARY

[0006] The main purpose of the present application is to provide an acid soil conditioner, which can significantly increase the pH value of acid soil, reduce the degree of soil acidification and improve the physical and chemical properties of soil.

[0007] The present application realizes the above-mentioned purpose by adopting the following technical solutions:

[0008] An acid soil conditioner is prepared mainly from the following raw materials by weight:

[0009] 10-15 parts of Bacillus subtilis, 10-15 parts of EM bacteria, 20-30 parts of ethylhexylglycerin, 3-8 parts of chitin, 10-15 parts of sodium tripolyphosphate, 25-35 parts of calcium magnesium phosphate fertilizer, 12-15 parts of allantoin, 20-30 parts of modified oyster shells, and 35-45 parts of modified brewer's grains.

[0010] Furthermore, in the acidic soil conditioner, the effective viable count of Bacillus subtilis is ≥20 billion / g, and the effective viable count of EM bacteria is ≥20 billion / g.

[0011] Furthermore, the preparation method of the modified oyster shell powder is as follows:

[0012] Oyster shell powder is soaked in a 10-15% calcium hydroxide solution for 10-12 hours, filtered, and calcined in an oxygen-free environment at 700-800℃ for 3-4 hours. Then, the temperature is lowered to 300-400℃, sepiolite is added, and the temperature is maintained for 1-1.5 hours. The temperature is then lowered to 200-250℃, molten polyethylene glycol is added, and the mixture is stirred. Then, sodium carboxymethyl cellulose is added, stirred evenly, cooled to room temperature, and pulverized to obtain modified oyster shell powder.

[0013] Furthermore, in the above-mentioned method for preparing modified oyster shell powder, the mass ratio of oyster shell powder, sepiolite, polyethylene glycol, and sodium carboxymethyl cellulose is 1:(0.1-0.15):(0.8-1):(0.3-0.5).

[0014] Furthermore, the preparation method of modified brewer's grains is as follows:

[0015] Inoculate brewer's grains with Aspergillus niger suspension at a material-to-liquid ratio of 1:0.3 (g / mL), adjust the pH to 7.0-7.5, and ferment at 25-30℃ for 1-1.5 days. Add water and boil, cool to room temperature, adjust the pH to 5.0-6.0, add cellulase, β-glucosidase, and Bacillus subtilis protease, and enzymatically hydrolyze at 35-40℃ for 6-8 hours. Boil again, cool to room temperature, and dry to obtain product I for later use. Add product I to 0.1mol / L NaOH solution at a material-to-liquid ratio of 1:30 (g / mL), incubate in a 40℃ water bath for 3-4 hours, filter, and add a mixed solution containing triethanolamine and polyvinylpyrrolidone at a material-to-liquid ratio of 1:4 (g / mL). Mix well and dry to obtain modified brewer's grains.

[0016] Furthermore, in the modified brewer's grains preparation method, the amount of Aspergillus niger spores in the Aspergillus niger suspension is 5 × 10⁻⁶. 10 per mL.

[0017] Further, in the modified brewer's grain preparation method, the cellulase is added in an amount of 5-8% of the mass of the brewer's grain, the beta-glucosidase is added in an amount of 4-7% of the mass of the brewer's grain, and the bacillus subtilis protease is added in an amount of 10-12% of the mass of the brewer's grain.

[0018] Further, in the modified brewer's grain preparation method, the mass fraction of triethanolamine in the mixed solution is 10-15%, and the mass fraction of polyvinylpyrrolidone is 25-30%.

[0019] The application provides a preparation method of the acid soil conditioner, comprising the following steps:

[0020] In step A, the bacillus subtilis, EM bacteria, chitin and ethylhexyl glycerin are mixed, dried, and crushed to obtain a mixture I;

[0021] In step B, the mixture I, sodium tripolyphosphate, calcium magnesium phosphate fertilizer, allantoin, modified oyster shell powder and modified brewer's grain are mixed to obtain the acid soil conditioner.

[0022] The application has the following beneficial effects:

[0023] 1. The main component of the oyster shell used in the application is calcium carbonate, which can be decomposed to produce calcium oxide after high-temperature calcination. The calcium oxide is sealed by polyethylene glycol and sodium carboxymethyl cellulose to avoid the reaction of calcium oxide with a large amount of water to generate strong base calcium hydroxide, which releases high heat and causes harm to crops. In actual use, the adsorption properties of polyethylene glycol, sodium carboxymethyl cellulose and sepiolite are used to preserve water, so that the water slowly contacts with the calcium oxide and the reaction is slow. Meanwhile, this is a long-term and continuous process, and a small amount of calcium hydroxide can be continuously generated to improve the soil pH for a long time.

[0024] 2. The alkaline modified brewer's grain is creatively added to the conditioner, and part of the macromolecular substances in the brewer's grain are decomposed into small molecular substances by enzymatic hydrolysis during the modification process, so that the nutritional components in the soil can be effectively increased. When the modified brewer's grain is used in the soil, the alkaline component can neutralize the acidic substances in the soil, and the modified brewer's grain has the dual effects of improving the soil pH and providing nutrient components.

[0025] 3. The microorganisms and alkaline substances in the application jointly act, and the microorganisms and the modified oyster shell powder both continuously and effectively improve the soil pH, which are the main components of the second gradient. The other components are the main components of the first gradient, which can reduce the soil acidity and provide nutrients for crops when used. DETAILED DESCRIPTION

[0026] The application will be further illustrated by specific examples, and it should be understood that the examples are only used to illustrate the application and not used to limit the scope of the application. After reading the application, those skilled in the art can make various equivalent modifications of the application, which fall within the scope of the claims of the present application. In the following examples, the preparation method of the Aspergillus niger suspension is as follows: the Aspergillus niger strain with the preservation number of CGMCC 3.15297 preserved in the China General Microbiological Culture Collection Center is activated according to the instructions, then expanded and cultured to prepare a seed liquid, and then diluted to prepare an Aspergillus niger suspension with a spore amount of 5×10 10 The cellulase has a CAS number of 9012-54-8 and an enzyme activity of 50 u / mg, the β-glucosidase has a CAS number of 9001-22-3 and an enzyme activity of 100 u / g, the bacillus subtilis protease has a CAS number of 9014-01-1 and an enzyme activity of 150 U / mg, and can be obtained through online or offline channels; the bacillus subtilis and the EM bacteria are both commercially available products, and in the present application, are purchased from Shandong Runwo Biotechnology Co., Ltd.

[0027] Example 1

[0028] Preparation of modified oyster shell powder:

[0029] Take oyster shells, crush and sieve to obtain oyster shell powder; 1 kg of the oyster shell powder is soaked in a calcium hydroxide solution with a mass fraction of 10% for 12 h, filtered, calcined in an oxygen-free environment at 800℃ for 3 h, then cooled to 400℃, 0.1 kg of sepiolite is added, and incubated for 1 h, then cooled to 250℃, 1 kg of polyethylene glycol in a molten state is added, then 0.3 kg of sodium carboxymethyl cellulose is added after mixing, uniformly mixed, and then cooled to room temperature, crushed, and the modified oyster shell powder is obtained.

[0030] Preparation of modified beer lees:

[0031] 1 kg of beer lees is inoculated with an Aspergillus niger suspension with a spore amount of 5×10 10 Individuals, adjusted to pH 7.5, and fermented at 30℃ for 1 day, then boiled with water, cooled to room temperature, adjusted to pH 6.0, and then 80 g of cellulase, 40 g of β-glucosidase, and 100 g of bacillus subtilis protease are added, and enzymolysis is carried out at 40℃ for 6 h, then boiled again, cooled to room temperature, dried, and the product I is obtained for standby use; the product I is added to a 0.1 mol / L NaOH solution according to a solid-liquid ratio of 1:30 (g / mL), incubated in a 40℃ water bath for 4 h, filtered, and then a mixed solution containing 15% triethanolamine and 25% polyvinylpyrrolidone is added according to a solid-liquid ratio of 1:4 (g / mL), uniformly mixed, and then dried, and the modified beer lees is obtained.

[0032] Preparation of an acidic soil conditioner:

[0033] Step A: Mix 15 kg of Bacillus subtilis with an effective viable count ≥ 20 billion / g, 10 kg of EM bacteria with an effective viable count ≥ 20 billion / g, 8 kg of chitin, and 20 kg of ethylhexylglycerin, dry, and pulverize to obtain mixture I;

[0034] Step B involves mixing Mixture I, 10 kg of sodium tripolyphosphate, 35 kg of calcium magnesium phosphate fertilizer, 12 kg of allantoin, 30 kg of modified oyster shell powder, and 35 kg of modified brewer's grains to obtain the acidic soil conditioner.

[0035] Example 2

[0036] Preparation of modified oyster shell powder:

[0037] Oyster shells were crushed and sieved to obtain oyster shell powder. 1 kg of oyster shell powder was soaked in a 15% calcium hydroxide solution for 10 hours, filtered, and calcined in an oxygen-free environment at 700℃ for 4 hours. The temperature was then lowered to 300℃, 0.15 kg of sepiolite was added, and the temperature was maintained for 1.5 hours. The temperature was then lowered to 200℃, 0.8 kg of molten polyethylene glycol was added, and the mixture was stirred. Then, 0.5 kg of sodium carboxymethyl cellulose was added, stirred evenly, cooled to room temperature, and crushed to obtain modified oyster shell powder.

[0038] Preparation of modified brewer's grains:

[0039] Inoculate 1 kg of brewer's grains at a material-to-liquid ratio of 1:0.3 (g / mL) with 5 × 10⁶ spores. 10 A suspension of Aspergillus niger per mL was prepared, and the pH was adjusted to 7.0. Fermentation was carried out at 25°C for 1.5 days. Water was added and the mixture was boiled. After cooling to room temperature, the pH was adjusted to 5.0. 50g of cellulase, 70g of β-glucosidase, and 120g of Bacillus subtilis protease were added. Enzymatic hydrolysis was carried out at 35°C for 8 hours. The mixture was boiled again, cooled to room temperature, and dried to obtain product I. Product I was then added to a 0.1mol / L NaOH solution at a material-to-liquid ratio of 1:30 (g / mL). The mixture was kept in a 40°C water bath for 3 hours, filtered, and then a mixed solution containing 10% triethanolamine and 30% polyvinylpyrrolidone was added at a material-to-liquid ratio of 1:4 (g / mL). After mixing and drying, modified brewer's grains were obtained.

[0040] Preparation of acidic soil conditioner:

[0041] Step A: Mix 10 kg of Bacillus subtilis with an effective viable count ≥ 20 billion / g, 15 kg of EM bacteria with an effective viable count ≥ 20 billion / g, 3 kg of chitin, and 30 kg of ethylhexylglycerin, dry, and pulverize to obtain mixture I;

[0042] Step B, mix mixture I, sodium tripolyphosphate 15 kg, calcium magnesium phosphate 25 kg, allantoin 15 kg, modified oyster shell powder 20 kg, modified beer lees 45 kg, to obtain the acidic soil conditioner.

[0043] Example 3

[0044] Preparation of modified oyster shell powder:

[0045] Take oyster shell, crush and sieve to obtain oyster shell powder; put 1 kg of oyster shell powder into a calcium hydroxide solution with a mass fraction of 13% and soak for 11 h, filter, calcine in an oxygen-free environment at 800℃ for 4 h, then cool to 300℃, add 0.13 kg of sepiolite, keep warm for 1.5 h, then cool to 250℃, add 0.9 kg of polyethylene glycol in molten state, then add 0.4 kg of sodium carboxymethyl cellulose, mix well, cool to room temperature, crush, to obtain the modified oyster shell powder.

[0046] Preparation of modified beer lees:

[0047] Put 1 kg of beer lees into a spore suspension of Aspergillus niger with a spore amount of 5×10 10 ML, adjust the pH to 7.0, cultivate at 30℃ for 1 day, boil with water, cool to room temperature, adjust the pH to 6.0, add 70 g of cellulase, 50 g of β-glucosidase and 110 g of subtilisin, enzymatically hydrolyze at 40℃ for 6 h, boil again, cool to room temperature, dry, to obtain product I for standby; put product I into 0.1 mol / L NaOH solution according to the solid-liquid ratio of 1:30 (g / mL), keep warm in a 40℃ water bath for 4 h, filter, add a mixed solution containing 13% triethanolamine and 28% polyvinylpyrrolidone according to the solid-liquid ratio of 1:4 (g / mL), mix well, dry, to obtain the modified beer lees.

[0048] Preparation of acidic soil conditioner:

[0049] Step A, mix 13 kg of Bacillus subtilis with effective viable count ≥200 billion / g, 12 kg of EM bacteria with effective viable count ≥200 billion / g, 6 kg of chitin and 25 kg of ethylhexyl glycerol, dry, crush, to obtain mixture I;

[0050] Step B, mix mixture I, 13 kg of sodium tripolyphosphate, 30 kg of calcium magnesium phosphate, 14 kg of allantoin, 25 kg of modified oyster shell powder and 40 kg of modified beer lees, to obtain the acidic soil conditioner.

[0051] Comparative Example 1

[0052] Preparation of modified beer lees:

[0053] The 1kg of beer lees was inoculated with 5×10 10 The beer lees was inoculated with 5×10

[0054] Preparation of the acid soil conditioner:

[0055] Step A: 13kg of Bacillus subtilis with effective viable count ≥200 billion / g, 12kg of EM bacteria with effective viable count ≥200 billion / g, 6kg of chitin, and 25kg of ethylhexyl glycerol were mixed, dried, and pulverized to obtain mixture I;

[0056] Step B: mixture I, 13kg of sodium tripolyphosphate, 30kg of calcium magnesium phosphate fertilizer, 14kg of allantoin, 25kg of oyster shell powder, and 40kg of modified beer lees were mixed to obtain the acid soil conditioner.

[0057] Comparative Example 2

[0058] Preparation of modified oyster shell powder:

[0059] The oyster shells were pulverized and sieved to obtain oyster shell powder. 1kg of the oyster shell powder was soaked in a calcium hydroxide solution with a mass fraction of 13% for 11h, filtered, calcined at 800℃ in an oxygen-free environment for 4h, then cooled to 300℃, 0.13kg of sepiolite was added and incubated for 1.5h, then cooled to 250℃, 0.9kg of polyethylene glycol in molten state was added, followed by 0.4kg of sodium carboxymethyl cellulose, mixed, and then cooled to room temperature. After pulverization, the modified oyster shell powder was obtained.

[0060] Preparation of the acid soil conditioner:

[0061] Step A: 13kg of Bacillus subtilis with effective viable count ≥200 billion / g, 12kg of EM bacteria with effective viable count ≥200 billion / g, 6kg of chitin, and 25kg of ethylhexyl glycerol were mixed, dried, and pulverized to obtain mixture I;

[0062] Step B, mix mixture I, sodium tripolyphosphate 13 kg, calcium magnesium phosphate 30 kg, modified oyster shell powder 25 kg, beer lees 40 kg, to obtain the acidic soil conditioner.

[0063] Comparative Example 3

[0064] Preparation of modified oyster shell powder:

[0065] Take oyster shell, crush and sieve to obtain oyster shell powder; put 1 kg of oyster shell powder into a 13% by mass calcium hydroxide solution and soak for 11 h, filter, calcine in an oxygen-free environment at 800℃ for 4 h, cool to room temperature, and crush to obtain the modified oyster shell powder.

[0066] Preparation of modified beer lees:

[0067] Inoculate 1 kg of beer lees with a spore amount of 5×10 10 spores / mL of Aspergillus niger suspension according to a solid-liquid ratio of 1:0.3 (g / mL), adjust the pH to 7.0, and ferment at 30℃ for 1 day. Boil with water, cool to room temperature, adjust the pH to 6.0, add cellulase 70 g, β-glucosidase 50 g, and bacillus subtilis protease 110 g, and enzymatically hydrolyze at 40℃ for 6 h. Boil again, cool to room temperature, dry, and obtain product I for standby; add 0.1 mol / L NaOH solution to product I according to a solid-liquid ratio of 1:30 (g / mL), and place in a 40℃ water bath for 4 h. Filter, add a mixed solution containing 13% by mass triethanolamine and 28% by mass polyvinylpyrrolidone according to a solid-liquid ratio of 1:4 (g / mL), mix well, and dry to obtain the modified beer lees.

[0068] Preparation of acidic soil conditioner:

[0069] Step A, mix bacillus subtilis with an effective viable cell number of ≥200 billion / g 13 kg, EM bacteria with an effective viable cell number of ≥200 billion / g 12 kg, chitin 6 kg, and ethylhexyl glycerol 25 kg, dry, crush, and obtain mixture I;

[0070] Step B, mix mixture I, sodium tripolyphosphate 13 kg, calcium magnesium phosphate 30 kg, modified oyster shell powder 25 kg, and modified beer lees 40 kg, to obtain the acidic soil conditioner.

[0071] Comparative Example 4

[0072] Preparation of modified oyster shell powder:

[0073] Take oyster shell, crush and sieve to obtain oyster shell powder; 1 kg of the oyster shell powder is soaked in a 13% by mass calcium hydroxide solution for 11 h, filtered, calcined in an oxygen-free environment at 800°C for 4 h, then cooled to 300°C, 0.13 kg of sepiolite is added and kept for 1.5 h, then cooled to 250°C, 0.9 kg of polyethylene glycol in molten state is added, then 0.4 kg of sodium carboxymethyl cellulose is added after mixing, uniformly mixed, then cooled to room temperature, and crushed to obtain modified oyster shell powder.

[0074] Preparation of modified beer lees:

[0075] The beer lees are added into a 0.1 mol / L NaOH solution according to a solid-liquid ratio of 1:30 (g / mL), and placed in a 40°C water bath for 4 h, filtered, and then a mixed solution containing 13% by mass triethanolamine and 28% by mass polyvinylpyrrolidone is added according to a solid-liquid ratio of 1:4 (g / mL), uniformly mixed, and dried to obtain modified beer lees.

[0076] Preparation of acidic soil conditioner:

[0077] The following are mixed to obtain the acidic soil conditioner: 13 kg of Bacillus subtilis with an effective viable count of ≥200 billion / g, 12 kg of EM bacteria with an effective viable count of ≥200 billion / g, 6 kg of chitin, 30 kg of calcium-magnesium phosphate fertilizer, 14 kg of allantoin, 25 kg of modified oyster shell powder, and 40 kg of modified beer lees.

[0078] Performance test

[0079] I. Effect of soil conditioner on soil physical and chemical properties and growth of Chinese cabbage

[0080] 1. Test and method

[0081] The test is carried out in an agricultural greenhouse in Linyi City. The soil properties are as follows: pH value 5.03, organic matter 19.6 g / kg, alkali-hydrolyzable nitrogen 127 mg / kg, available phosphorus 86.2 mg / kg, available potassium 138 mg / kg, exchangeable calcium 519 mg / kg, exchangeable magnesium 72.4 mg / kg, chromium 0.402 mg / kg, lead 23.7 mg / kg, mercury 0.0125 mg / kg, and arsenic 0.241 mg / kg.

[0082] The soil is treated as follows and then loaded into the seedling pots at 2 kg per pot, and placed at room temperature for 10 days of culture.

[0083] Blank group: soil + ordinary organic fertilizer (10 g / kg);

[0084] Test 1 group: soil + ordinary organic fertilizer (10 g / kg) + soil conditioner of Example 1 (10 g / kg);

[0085] Test 2 group: soil + ordinary organic fertilizer (10 g / kg) + soil conditioner of Example 2 (10 g / kg);

[0086] Test 3 group: soil + ordinary organic fertilizer (10 g / kg) + soil conditioner of Example 3 (10 g / kg);

[0087] Test 4 group: soil + ordinary organic fertilizer (10 g / kg) + soil conditioner of Comparative Example 1 (10 g / kg);

[0088] Test 5 group: soil + ordinary organic fertilizer (10 g / kg) + soil conditioner of Comparative Example 2 (10 g / kg);

[0089] Test 6 group: soil + ordinary organic fertilizer (10 g / kg) + soil conditioner of Comparative Example 3 (10 g / kg);

[0090] Test 7 group: soil + ordinary organic fertilizer (10 g / kg) + soil conditioner of Comparative Example 4 (10 g / kg);

[0091] Each of the above groups is set up with 50 pots, and repeated twice.

[0092] After the soil culture is completed, the small cabbage seedlings with substantially the same growth are transplanted into the seedling pots, and harvested after growing for 20 days. 20 pots are randomly selected from each group to collect soil samples, and the contents of soil pH and organic matter, available phosphorus, available potassium, etc. are determined according to the “Soil Agricultural Chemistry Analysis Method”; 20 pots are randomly selected from each group to determine the growth traits of the small cabbage.

[0093] 2. Results and analysis

[0094] 2.1 Effect of soil conditioner on soil properties

[0095] As can be seen from Table 1, the application of the soil conditioner of the application can significantly increase the pH value of the soil, reduce the soil acidity, and increase the contents of exchangeable calcium and exchangeable magnesium in the soil, and the effect is better than that of using the soil conditioners of Comparative Examples 1-4.

[0096] Table 1 Effect of soil conditioner on soil physicochemical properties

[0097] Group pH Exchangeable calcium (mg / kg) Exchangeable magnesium (mg / kg) Control group 5.08 525 71.3 Test 1 group 5.59 732 80.3 Test 2 group 5.71 701 78.6 Test 3 group 5.68 695 81.5 Test 4 group 5.13 594 73.9 Test 5 group 5.15 568 72.5 Test 6 group 5.11 572 76.3 Test 7 group 5.12 549 75.1

[0098] Table 2 Effect of soil conditioner on soil nutrient content

[0099] Group Alkaline nitrogen (mg / kg) Available phosphorus (mg / kg) Available potassium (mg / kg) Organic matter (g / kg) Control group 131 85.9 135 20.3 Test 1 group 171 143.6 176 26.8 Test 2 group 173 129.5 183 25.1 Test 3 group 168 136.8 169 25.9 Test 4 group 145 98.3 159 22.3 Test 5 group 133 96.7 141 21.2 Test 6 group 138 101.2 153 21.8 Test 7 group 142 93.5 146 22.6

[0100] Table 3 Effect of soil conditioner on soil heavy metal content

[0101] Group Chromium (mg / kg) Lead (mg / kg) Mercury (mg / kg) Arsenic (mg / kg) Control group 0.399 23.3 0.0126 0.245 Test 1 group 0.264 14.6 0.0072 0.164 Test 2 group 0.273 13.1 0.0051 0.179 Test 3 group 0.268 15.8 0.0065 0.153 Test 4 group 0.385 20.3 0.0105 0.227 Test 5 group 0.381 21.1 0.0120 0.205 Test 6 group 0.373 21.5 0.0113 0.223 Test 7 group 0.378 20.9 0.0115 0.216

[0102] From the results in Table 2, Table 3, it can be seen that after applying the soil conditioner of the present application, the content of each nutrient in the soil can be obviously increased, the content of nitrogen, phosphorus, potassium and organic matter can be increased; and the content of heavy metals in the soil can be effectively reduced.

[0103] 2.2 Effect of the soil conditioner on the growth of Chinese flowering cabbage

[0104] From the results in Table 4 below, it can be seen that after the soil is improved by the soil conditioner of the present application, the overall growth of Chinese flowering cabbage is better, the weight of single plant is higher, the leaf weight and leaf area are larger, and the petiole is shorter.

[0105] Table 4 Effect of the soil conditioner on the growth of Chinese flowering cabbage

[0106]

[0107] II. Effect of the soil conditioner on the growth of peanuts

[0108] 1. Test and method

[0109] The test was carried out in an agricultural planting base in Linyi City. The soil properties are as follows: pH value 4.98, organic matter 23.5 g / kg, alkali-hydrolyzable nitrogen 136.2 mg / kg, available phosphorus 12.6 mg / kg, available potassium 162.5 mg / kg, exchangeable calcium 495.8 mg / kg, exchangeable magnesium 70.3 mg / kg, chromium 16.2 mg / kg, cadmium 0.085 mg / kg, lead 32.8 mg / kg, mercury 0.026 mg / kg, and arsenic 0.84 mg / kg.

[0110] Eight treatments were set up according to the following methods respectively:

[0111] Blank field: conventional fertilization;

[0112] Test field 1: conventional fertilization + soil conditioner of Example 1 (600 kg / hm 2 );

[0113] Test field 2: conventional fertilization + soil conditioner of Example 2 (600 kg / hm 2 );

[0114] Test field 3: conventional fertilization + soil conditioner of Example 3 (600 kg / hm 2 );

[0115] Test field 4: conventional fertilization + soil conditioner of Comparative Example 1 (600 kg / hm 2 );

[0116] Test field 5: conventional fertilization + soil conditioner of Comparative Example 2 (600 kg / hm 2 );

[0117] Test field 6: conventional fertilization + comparative example 3 soil conditioner (600 kg / hm 2 );

[0118] Test field 7: conventional fertilization + comparative example 4 soil conditioner (600 kg / hm 2 );

[0119] The area of each treatment plot is 0.005 hm 2 Each treatment is applied as a base fertilizer once, is turned into the ground when plowing, is sowed after 15 h, is planted in double rows and double grains, and the sowing density is 100000 holes / hm 2 After planting, normal management measures are carried out for field management.

[0120] At the peanut harvesting period, 5 soil samples are randomly collected in each plot by using the "S" type sampling method, and the soil properties are determined; after the peanuts in each plot are harvested, the peanut traits such as single plant fruit number, kilogram fruit number, and full fruit rate are investigated.

[0121] 2. Results and analysis

[0122] 2.1 Influence of the soil conditioner on the soil properties of the peanut field

[0123] Table 5 Influence of different soil conditioners on the pH value and chemical properties of the soil in the peanut field

[0124]

[0125]

[0126] Table 6 Influence of different soil conditioners on the heavy metal content in the soil in the peanut field

[0127] Group Chromium (mg / kg) Cadmium (mg / kg) Lead (mg / kg) Mercury (mg / kg) Arsenic (mg / kg) Control group 15.8 0.086 31.5 0.023 0.081 Test field 1 13.2 0.053 26.4 0.009 0.057 Test field 2 11.8 0.061 25.8 0.011 0.051 Test field 3 12.5 0.059 27.3 0.013 0.046 Test field 4 15.3 0.077 29.6 0.021 0.073 Test field 5 14.9 0.081 28.1 0.016 0.069 Test field 6 15.7 0.073 30.5 0.019 0.073 Test field 7 15.1 0.076 28.9 0.015 0.065

[0128] From the results in the above table 5 and table 6, it can be known that the soil conditioner of the present application can effectively reduce soil acidity, increase the content of nutrient components, and reduce the content of heavy metals.

[0129] 2.2 Influence of different soil conditioner treatments on the peanut traits

[0130] From the results in the following table 7, it can be known that the soil applying the conditioner of the present application is beneficial to the growth of peanuts, and the single plant fruit number, full fruit rate, and yield of peanuts are all obviously increased.

[0131] Table 7 Influence of different soil conditioner treatments on the peanut traits

[0132] Group Number of single plant results Saturation fruit rate (%) Kilogram of fruit Yield (kg / hm 2 )]]> Control group 16.3 86.39 521.3 6123.14 Test field 1 20.3 93.25 476.3 7352.48 Test field 2 19.5 92.14 489.5 7252.36 Test field 3 21.1 93.02 481.5 7302.05 Test field 4 17.2 88.57 512.3 6859.74 Test field 5 18.4 89.41 509.7 6714.52 Test field 6 17.9 90.42 496.8 6802.59 Test field 7 18.3 89.73 504.2 6635.48

Claims

1. An acid soil conditioner, characterized in that, It is prepared from the following raw materials by weight: Bacillus subtilis 10-15 parts, EM bacteria 10-15 parts, ethylhexyl glycerol 20-30 parts, chitin 3-8 parts, sodium tripolyphosphate 10-15 parts, calcium magnesium phosphate fertilizer 25-35 parts, allantoin 12-15 parts, modified oyster shell 20-30 parts, modified beer dregs 35-45 parts; The effective viable count of the Bacillus subtilis is ≥200 billion / g, and the effective viable count of the EM bacteria is ≥200 billion / g; The preparation method of the modified oyster shell powder is: The oyster shell powder is soaked in a calcium hydroxide solution with a mass fraction of 10-15% for 10-12 hours, filtered, calcined in an oxygen-free environment at 700-800°C for 3-4 hours, then cooled to 300-400°C, sea sponges are added and kept at temperature for 1-1.5 hours, then cooled to 200-250°C, molten polyethylene glycol is added, mixed, then sodium carboxymethyl cellulose is added, mixed, cooled to room temperature, and then crushed to obtain the modified oyster shell powder; the mass ratio of the oyster shell powder, sea sponges, polyethylene glycol, and sodium carboxymethyl cellulose is 1:(0.1-0.15):(0.8-1):(0.3-0.5); The preparation method of the modified beer dregs is: The beer dregs are inoculated with spore suspension of Aspergillus niger at a ratio of 1:0.3 (g / mL) and a spore amount of 5×10 10 The beer dregs are inoculated with spore suspension of Aspergillus niger at a ratio of 1:0.3 (g / mL) and a spore amount of 5×10 10 The beer dregs are inoculated with spore suspension of Aspergillus niger at a ratio of 1:0.3 (g / mL) and a spore amount of 5×10 10 The beer dregs are inoculated with spore suspension of Aspergillus niger at a ratio of 1:0.3 (g / mL) and a spore amount of 5×10 10 The beer dregs are inoculated with spore suspension of Aspergillus niger at a ratio of 1:0.3 (g / mL) and a spore amount of 5×10 10 The beer dregs are inoculated with spore suspension of Aspergillus niger at a ratio of 1:0.3 (g / mL) and a spore amount of 5×10 10 The beer dregs are inoculated with spore suspension of Aspergillus niger at a ratio of 1:0.3 (g / mL) and a spore amount of 5×10 10 The beer dregs are inoculated with spore suspension of Aspergillus niger at a ratio of 1:0.3 (g / mL) and a spore amount of 5×10 10 The beer dregs are inoculated with spore suspension of Aspergillus niger at a ratio of 1:0.3 (g / mL) and a spore amount of 5×10 10 The beer dregs are inoculated with spore suspension of Aspergillus niger at a ratio of 1:0.3 (g / mL) and a spore amount of 5×10 10 The beer dregs are inoculated with spore suspension of Aspergillus niger at a ratio of 1:0.3 (g / mL) and a spore amount of 5×10 10 The beer dregs are inoculated with spore suspension of Aspergillus niger at a ratio of 1:0.3 (g / mL) and a spore amount of 5×10 10 The beer dregs are inoculated with spore suspension of Aspergillus niger at a ratio of 1:0.3 (g / mL) and a spore amount of 5×10 10 The beer dregs are inoculated with spore suspension of Aspergillus niger at a ratio of 1:0.3 (g / mL) and a spore amount of 5×10 10 The beer dregs are inoculated with spore suspension of Aspergillus niger at a ratio of 1:0.3 (g / mL) and a spore amount of 5×10 10 The beer dregs are inoculated with spore suspension of Aspergillus niger at a ratio of 1:0.3 (g / mL) and a spore amount of 5×10 10 The beer dregs are inoculated with spore suspension of Aspergillus niger at a ratio of 1:0.3 (g / mL) and a spore amount of 2. A method of preparing the acid soil conditioner of claim 1, characterized by, The method comprises the following steps: Step A: Bacillus subtilis, EM bacteria, chitin, and ethylhexyl glycerol are mixed, dried, and crushed to obtain a mixture I; Step B: The mixture I, sodium tripolyphosphate, calcium magnesium phosphate fertilizer, allantoin, modified oyster shell powder, and modified beer dregs are mixed to obtain the acid soil conditioner.

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