A constant temperature shaker for vaccine shake flask cultivation

Abstract

The present application belongs to the technical field of oscillator, especially a constant-temperature oscillator for vaccine shake flask culture, aiming at the problem that the existing constant-temperature oscillator cannot clamp and fix the shake flask, and the corresponding water bath constant-temperature oscillator and air bath constant-temperature oscillator need to be used, thereby greatly increasing the culture cost, the present application proposes the following scheme, including a box body, a shaking plate is arranged in the box body, a plurality of clamping members for clamping the shake flask are arranged on the top of the shaking plate, U-shaped handles are fixed on the two sides of the top of the shaking plate, the shaking plate is lifted by the U-shaped handles, a temperature sensor is fixed on the inner wall of the bottom of the box body, a water tank and a heating tank are further included, the water tank is fixed on the bottom of the box body, the present application can perform air bath and water bath on the shake flask on the same equipment, the water bath and the air bath can be freely switched by the switching structure, the operation is simple, the cost of vaccine shake flask culture is greatly reduced, and the shake flask can be clamped in all directions by the cooperation of the clamping members, the first spring and the pressing plate during shaking.

Description

Technical Field

[0001] This invention relates to the field of oscillator technology, and more particularly to a constant temperature oscillator for vaccine shake flask culture. Background Technology

[0002] Constant-temperature shakers for vaccine shake flask culture are widely used in biology, medicine, agricultural science, and other fields, playing a particularly important role in vaccine production, cell culture, and microbial fermentation. During vaccine culture, the appropriate shaker, such as a water bath constant-temperature shaker or an air bath constant-temperature shaker, is required depending on the type of vaccine being cultured.

[0003] In the existing technology, the constant temperature oscillator still has the following disadvantages during use:

[0004] 1. When shaking a flask containing a vaccine, the flask is usually placed directly in the placement ring on the shaking plate. Since the flask cannot be clamped and fixed, the flask will shake a lot in the placement ring when the shaking plate is running, which can easily cause the flask to break.

[0005] 2. Different vaccines require the use of corresponding water bath and air bath constant temperature shakers. Therefore, when performing vaccine shake flask culture, it is necessary to prepare the corresponding constant temperature shaker, which greatly increases the cost of vaccine shake flask culture.

[0006] To address the above problems, this invention proposes a constant temperature shaker for vaccine shake flask culture. Summary of the Invention

[0007] The purpose of this invention is to solve the problem that existing shake flasks cannot be clamped and fixed, requiring the use of corresponding water bath constant temperature shakers and air bath constant temperature shakers, which greatly increases the culture cost. Therefore, this invention proposes a constant temperature shaker for vaccine shake flask culture.

[0008] To achieve the above objectives, the present invention adopts the following technical solution:

[0009] A constant temperature shaker for vaccine shake flask culture includes a housing, a shaking plate inside the housing, a plurality of clamping components for holding the shake flask on the top of the shaking plate, U-shaped handles fixed on both sides of the top of the shaking plate for lifting the shaking plate, and a temperature sensor fixed on the bottom inner wall of the housing.

[0010] It also includes a water tank and a heating tank. The water tank is fixed to the bottom of the tank body and is used to inject hot water into the tank body for water bath heating. The heating tank is fixed to the top of the water tank and is fixedly connected to one side of the tank body for injecting hot air into the tank body for air bath heating.

[0011] It also includes a top cover set on the top of the box for closing the box, and a sliding plate extending into the box is slidably provided at the bottom of the top cover, and the sliding plate cooperates with the oscillating plate to clamp the shaker from the vertical direction.

[0012] It also includes a rotating rod that rotates inside the box, and the rotating rod is located below the oscillating plate;

[0013] The clamping structure, located inside the sliding plate, is used to cooperate with the oscillating plate and clamping components to clamp the shaker flask in all directions.

[0014] An oscillating structure is located on one side of the housing and is used to drive the rotating rod to rotate and complete the reciprocating oscillating movement of the oscillating plate.

[0015] The water bath heating structure is installed inside the water tank and is used to inject hot water into the tank for water bath heating;

[0016] The air bath heating structure is installed inside the heating box and is used to inject hot air into the box for air bath heating. The oscillating structure can drive the air bath heating structure to operate through the rotating rod.

[0017] The switching structure, located inside the enclosure, is used to control the opening and closing of the air bath heating structure, and the water bath heating structure can also be used to drive the switching structure.

[0018] In one possible design, the clamping structure includes multiple insertion holes disposed at the bottom of the sliding plate, each of the multiple insertion holes having a first spring fixed to its top inner wall, and each of the multiple insertion holes having a pressure plate slidably connected to abutting the top of the shaker bottle, the top of the pressure plate being fixedly connected to the bottom end of the first spring, and the elastic force of the first spring on the pressure plate being able to clamp the shaker bottle placed on the oscillating plate in the vertical direction.

[0019] The clamping component includes multiple vertical plates and clamping plates. The vertical plates are fixed to the top of the oscillating plate, and the clamping plates are fixed to the top of the vertical plates. Multiple spring plates are fixed to the side of the clamping plate near the shake bottle. The multiple spring plates on the clamping plates cooperate to clamp the shake bottle. A rubber pad is provided on the side of the spring plates near the shake bottle for protection. The shake bottle containing the vaccine is placed in the clamping component. The shake bottle is initially clamped by the cooperation of the vertical plates, clamping plates, and spring plates. The box is sealed by the top cover. The top of the shake bottle is inserted into the insertion hole and abuts against the bottom of the pressure plate. The elastic force of the first spring on the pressure plate can cooperate with the oscillating plate to clamp the shake bottle vertically. Therefore, the cooperation of the clamping component with the pressure plate and the oscillating plate clamps and fixes the shake bottle, ensuring the stability of the shake bottle during subsequent oscillation.

[0020] In one possible design, the oscillation structure includes a drive motor fixed to one side of the housing via a frame. The output shaft of the drive motor is fixedly connected to one end of a rotating rod via a coupling. The outer wall of the rotating rod has multiple reciprocating threaded sections. Multiple moving blocks are threadedly connected to the rotating rod via these reciprocating threaded sections, and the moving blocks slide on the bottom inner wall of the housing to drive the moving blocks to reciprocate. Multiple U-shaped retaining sleeves are fixed to the bottom of the oscillation plate, and these sleeves are fitted onto the outer wall of the moving blocks to drive the oscillation plate to reciprocate linearly via the moving blocks. Multiple guide blocks are fixed to both sides of the oscillation plate. Multiple first slides are provided on the inner walls of the two opposite sides of the housing, and the guide blocks slide in conjunction with the first slides to... When the oscillating plate is placed inside the housing, the moving block and the U-shaped clamp are properly aligned. The inner walls of the housing on both sides away from each other are provided with second slides, which slide in conjunction with guide blocks. The second slides are located below the first slides, and the bottoms of multiple first slides on the same side are connected to adjacent second slides, facilitating the reciprocating movement of the oscillating plate later. When the oscillating plate is placed into the housing along the first slide, the U-shaped clamp fits perfectly onto the moving block. The rotating rod drives the moving block to reciprocate through the reciprocating thread section. The engagement between the moving block and the U-shaped clamp drives the oscillating plate and the shaker on it to reciprocate. Furthermore, when the oscillating plate moves the shaker, the top of the shaker simultaneously moves the sliding plate, ensuring stability during the shaker's oscillation process.

[0021] In one possible design, the water bath heating structure includes multiple first heating elements fixed to the inner wall of the bottom of the water tank for heating the water in the tank. A water pump is fixed to the inner wall of the bottom of the water tank via a frame. An outlet pipe is fixed to the outlet end of the water pump, and the top end of the outlet pipe extends into the tank for injecting hot water into the tank. A return pipe is fixedly connected to the bottom of the tank, and the bottom end of the return pipe extends into the water tank. The outlet pipe and the return pipe cooperate to circulate the hot water in the tank and the water body. The water in the tank is heated by the first heating elements, and the water pump injects hot water into the tank through the outlet pipe. When the shaker inside the tank is being heated in a water bath, the solenoid valve on the return pipe is opened to circulate the hot water in the tank and the water body, ensuring stable water temperature.

[0022] In one possible design, the air bath heating structure includes a mounting bracket fixed inside a heating chamber. A rotating shaft rotatably passes through the mounting bracket. A fan and a first bevel gear are fixed to the top and bottom of the rotating shaft, respectively. Multiple second heating elements located above the fan are fixed to the inner walls of the two opposite sides of the heating chamber for heating the air. An air outlet pipe is fixedly connected to the top of the heating chamber, with one end extending into the chamber to inject hot air into it. A rotating shaft located below the first bevel gear is rotatably connected inside the heating chamber. A second bevel gear is slidably connected to the outer wall of the rotating shaft via a groove and a slider, and the second bevel gear meshes with the first bevel gear. The side of the second bevel gear closest to the chamber has a... The annular groove has a return air pipe fixedly connected to one side of the chamber. One end of the return air pipe is fixedly connected to the heating chamber, and the connection between the return air pipe and the heating chamber is located below the fan. The end of the rotating rod away from the drive motor extends into the heating chamber in a sealed manner and is fixedly connected to one end of the rotating shaft to drive the second bevel gear to rotate. The air inside the heating chamber is heated by the second heating element. Then, the drive motor drives the rotating rod to rotate. The rotating rod drives the second bevel gear and the fan to rotate through the rotating shaft. The solenoid valves on the outlet and return air pipes are opened, and the fan injects the hot air inside the heating chamber into the chamber through the outlet air pipe. The cold air inside the chamber flows back into the heating chamber through the return air pipe for reheating, thus completing the air circulation and performing air bath heating on the shaker.

[0023] In one possible design, the switching structure includes multiple vertical rods fixed to the inner wall of the bottom of the tank, with the vertical rods located below the rotating rod. A common float plate is slidably fitted onto the outer wall of the multiple vertical rods, and the float plate has a trapezoidal cross-section to increase the contact area with water. A connecting rod is rotatably connected to the side of the float plate near the heating tank, and a push rod is rotatably connected to the top of the connecting rod. One end of the push rod slidably penetrates one inner wall of the tank and extends into the heating tank, while the other end extends into an annular groove and slidably engages with the annular groove. The raising and lowering of the float plate controls the push rod and the second bevel gear. The system moves to control the engagement and disengagement of the second and first bevel gears. Water is injected into the tank through the outlet pipe. As the water level rises, the float moves upward under buoyancy. The float, via a connecting rod, pushes the push rod and solenoid valve to move away from the tank, disengaging the second and first bevel gears. This allows the fan to be deactivated during water bathing. Conversely, the float moves downward under its own weight, causing the connecting rod to rotate. The connecting rod, via the push rod, pulls the solenoid valve to one side. When the rotating shaft drives the second bevel gear to rotate, it engages with the first bevel gear, allowing hot air to be injected into the tank for air bathing.

[0024] In one possible design, limiting plates are fixed on both sides of the bottom of the top cover. The side of the two limiting plates that are far apart from each other is attached to the inner wall of the corresponding side of the box body to increase the stability of the top cover in closing the box body. Multiple sliding rods are fixed on the side of the two limiting plates that are close to each other. One end of each of the multiple sliding rods slides into the sliding plate. Multiple second springs are fixed between the two limiting plates and the sliding plate. The multiple second springs are respectively sleeved on the outer wall of the corresponding sliding rod to reset the sliding plate to the center position of the bottom of the top cover, so that when the top cover closes the box body, the top of the shaker can be inserted into the insertion hole.

[0025] In one possible design, hemispherical rubber strips are fixed to the sides of the two limiting plates that are far apart from each other, and slots are provided on the inner walls of the two sides of the box that are close to each other, and the hemispherical rubber strips are engaged with the slots to ensure the stability of the top cover when the top cover closes the box.

[0026] In one possible design, the outer walls of the outlet pipe, return pipe, and return liquid pipe are all equipped with solenoid valves.

[0027] In one possible design, the oscillating plate has multiple cavities, and multiple limiting rods slide through the oscillating plate, with one end of each limiting rod penetrating a corresponding cavity. The top of each limiting rod has a trapezoidal groove, and the top inner wall of each cavity has multiple pushing platforms that slide through, with the top of each pushing platform extending above the oscillating plate. Each pushing platform is located within a plurality of clamping components, and the bottom of each pushing platform is fixed with a fixing column. The bottom end of each fixing column is arc-shaped and engages with the trapezoidal groove to drive the pushing platform and fixing column to reciprocate up and down. The two ends of each limiting rod abut against the inner walls of the housing on opposite sides. The bottom sides of each limiting rod have inclined surfaces to facilitate placing the oscillating plate and limiting rods within the housing. During the process of the moving block driving the oscillating plate to reciprocate and drive the shaker to oscillate laterally, the cooperation of the fixing column, trapezoidal groove, and first spring drives the shaker to reciprocate longitudinally, increasing the shaking effect.

[0028] Beneficial effects: In this invention, a first spring is fixed to the top inner wall of each of the multiple insertion holes, and a pressure plate is slidably connected to the bottom end of the first spring in each of the multiple insertion holes. The clamping component consists of multiple vertical plates and clamping plates. The clamping plate is fixed to the top of the vertical plate, and multiple spring plates are fixed to the side of the clamping plate near the shake bottle. The shake bottle placed in the clamping component is clamped by the cooperation of the vertical plate, clamping plate and spring plates. The top cover closes the box. The elastic force of the first spring on the pressure plate can cooperate with the oscillation plate to clamp the shake bottle vertically. Therefore, the shake bottle can be fully clamped and fixed to ensure the stability of the shake bottle during subsequent oscillation.

[0029] In this invention, the outer walls of multiple vertical rods are slidably fitted with the same vertical rod. A connecting rod is rotatably connected to one side of the float plate, and a push rod is rotatably connected to the top of the connecting rod. One end of the push rod is sealed and slidably penetrates one side of the inner wall of the box and slides in cooperation with the annular groove. The buoyancy of the water on the float plate can control the left and right movement of the second bevel gear. Thus, during water bathing, the second bevel gear disengages from the first bevel gear, and conversely, the second bevel gear engages with the first bevel gear, thereby completing the free switching between water bath and air bath, which is simple to operate.

[0030] In this invention, the oscillating plate has multiple cavities, and multiple limiting rods slide through the oscillating plate. Each of the limiting rods has a trapezoidal groove at its top, and multiple pushing platforms slide through the top inner walls of each of the cavities. Each of the pushing platforms has a fixed column at its bottom. During the process of the moving block driving the oscillating plate to reciprocate and drive the shaker to oscillate laterally, the cooperation of the fixed column, the trapezoidal groove, and the first spring can drive the shaker to reciprocate longitudinally, thereby increasing the shaking effect.

[0031] In this invention, air baths and water baths can be performed on the same device, and the switching structure can be used to freely switch between water baths and air baths. The operation is simple and greatly reduces the cost of vaccine shake culture. In addition, during oscillation, the shake flask can be clamped in all directions by the cooperation of the clamping component, the first spring, and the pressure plate, ensuring the stability of the shake flask. Attached Figure Description

[0032] Figure 1 A three-dimensional structural schematic diagram of a constant temperature shaker for vaccine shake flask culture provided by the present invention;

[0033] Figure 2 A cross-sectional schematic diagram of a constant-temperature shaker for vaccine shake flask culture provided by the present invention;

[0034] Figure 3 A three-dimensional cross-sectional view of the housing of a constant-temperature shaker for vaccine shake culture provided by the present invention;

[0035] Figure 4 This is a three-dimensional exploded view of the top cover, sliding plate, pressure plate and limiting plate of a constant temperature shaker for vaccine shake culture provided by the present invention;

[0036] Figure 5 This is a three-dimensional structural diagram of the shaking plate and clamping components of a constant temperature shaker for vaccine shake flask culture provided by the present invention;

[0037] Figure 6 This is a three-dimensional exploded structural diagram of the clamping component of a constant temperature shaker for vaccine shake culture provided by the present invention.

[0038] Figure 7 A three-dimensional exploded cross-sectional view of the shaking plate and moving block of a constant-temperature shaker for vaccine shake culture provided by the present invention.

[0039] Figure 8 This is a three-dimensional exploded cross-sectional view of the heating box, mounting frame, and rotating shaft of a constant temperature shaker for vaccine shake culture provided by the present invention.

[0040] Figure 9 A three-dimensional exploded view of the float, connecting rod, and push rod of a constant temperature shaker for vaccine shake culture provided by the present invention;

[0041] Figure 10 A three-dimensional cross-sectional view of the housing and shaking plate of a constant temperature shaker for vaccine shake culture provided by the present invention;

[0042] Figure 11 This is a cross-sectional schematic diagram of the shaking plate and limiting rod of a constant temperature shaker for vaccine shake culture provided by the present invention.

[0043] In the diagram: 1. Box body; 2. Vibrating plate; 3. Clamping component; 4. Vertical plate; 5. Clamping plate; 6. Spring sheet; 7. Top cover; 8. Sliding plate; 9. Insertion hole; 10. First spring; 11. Pressure plate; 12. Limiting plate; 13. Slide rod; 14. Second spring; 15. Hemispherical rubber strip; 16. Slot; 17. First slide rail; 18. Second slide rail; 19. Guide block; 20. Rotating rod; 21. Drive motor; 22. Reciprocating threaded section; 23. Moving block; 24. Return sleeve; 25. Water tank; 26. Water pump; 27. Outlet 28. Liquid pipe; 29. ​​Temperature sensor; 30. First heating element; 31. Liquid return pipe; 32. Heating box; 33. Gas outlet pipe; 34. Gas return pipe; 35. Second heating element; 36. Mounting bracket; 37. Rotating shaft; 38. First bevel gear; 39. Rotating shaft; 40. Second bevel gear; 41. Push rod; 42. Vertical rod; 43. Float plate; 44. Connecting rod; 45. Limiting rod; 46. Cavity; 47. Pushing platform; 48. Fixed column; 49. Trapezoidal groove; 50. Solenoid valve; 51. U-shaped handle; 52. Annular groove. Detailed Implementation

[0044] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0045] Example 1: Refer to Figure 1 , Figure 2 and Figure 5This invention relates to the field of oscillator technology. The constant-temperature oscillator includes a housing 1, inside which an oscillating plate 2 is disposed. The top of the oscillating plate 2 is designed with multiple clamping members 3 for securely clamping vaccine shakers. Furthermore, U-shaped handles 51 are fixed on both sides of the top of the oscillating plate 2, allowing users to easily lift the oscillating plate 2 for loading or unloading vaccine shakers.

[0046] Reference Figure 1 A temperature sensor 28 is fixed on the bottom inner wall of the chamber 1 to monitor the temperature inside the chamber 1 in real time.

[0047] To provide two heating methods, namely water bath heating and air bath heating, we designed the following structure:

[0048] Reference Figure 1 and Figure 2 The water tank 25 is fixed to the bottom of the tank body 1. Its function is to inject hot water into the tank body 1 for water bath heating. The heating box 31 is fixed to the top of the water tank 25 and is fixedly connected to one side of the tank body 1. The function of the heating box 31 is to inject hot air into the tank body 1 for air bath heating.

[0049] Reference Figure 1 and Figure 2 To seal the box 1, we installed a top cover 7 on its top. A sliding plate 8 is slidably connected to the bottom of the top cover 7. This sliding plate 8 can extend into the inside of the box 1 and cooperate with the shaking plate 2 to clamp the vaccine shaker from the vertical direction.

[0050] Reference Figure 1 Inside the housing 1, there is also a rotating rod 20, which is located below the vibrating plate 2.

[0051] Reference Figure 4 Limiting plates 12 are fixed on both sides of the bottom of the top cover 7. These two limiting plates 12 are located on the left and right sides of the bottom of the top cover 7, respectively. The outer sides of the two limiting plates 12 that are far apart from each other are tightly fitted to the inner wall of the corresponding side of the box 1. This design increases the stability of the top cover 7 when it is closed to the box 1, and prevents the top cover 7 from shaking or misaligning during the closing process.

[0052] Reference Figure 4Meanwhile, multiple sliding rods 13 are fixed to the inner sides of the two limiting plates 12 that are close to each other. One end of each sliding rod 13 extends slidably into the sliding plate 8, allowing the sliding plate 8 to slide on the sliding rod 13. To maintain the stability of the sliding plate 8 at the bottom center position of the top cover 7, multiple second springs 14 are fixed between the two limiting plates 12 and the sliding plate 8. These second springs 14 are respectively sleeved on the outer wall of the corresponding sliding rod 13. When the sliding plate 8 is subjected to external force and deviates from the center position, the second springs 14 will provide elastic force to return the sliding plate 8 to the bottom center position of the top cover 7. In this way, when the top cover 7 closes the box 1, the top of the shaker can be more easily inserted into the insertion hole 9.

[0053] Reference Figure 3 and Figure 4 On the side of the two limiting plates 12 that are far apart from each other, there are also hemispherical rubber strips 15. These hemispherical rubber strips 15 have a certain elasticity and friction, and can be engaged with the slots 16 on the inner walls of the two sides of the box 1 that are close to each other. When the top cover 7 closes the box 1, the hemispherical rubber strips 15 will be engaged in the slots 16, thereby increasing the stability and sealing between the top cover 7 and the box 1.

[0054] Next, we will describe the clamping structure in detail:

[0055] Reference Figures 4-6 The bottom of the sliding plate 8 is designed with multiple insertion holes 9, and a first spring 10 is fixed to the inner wall of the top of each insertion hole 9. A pressure plate 11 is slidably connected inside each insertion hole 9, and the top of the pressure plate 11 is fixedly connected to the bottom end of the first spring 10. When the vaccine shake bottle is placed on the shaking plate 2, the elastic force of the first spring 10 on the pressure plate 11 can firmly clamp the vaccine shake bottle in the vertical direction.

[0056] Reference Figure 5 and Figure 6 The clamping component 3 includes multiple vertical plates 4 and a clamping plate 5. The vertical plates 4 are fixed to the top of the vibrating plate 2, while the clamping plate 5 is fixed to the top of the vertical plates 4. Multiple spring plates 6 are fixed to the side of the clamping plate 5 near the vaccine shake bottle. These spring plates 6 cooperate with each other to clamp the vaccine shake bottle. A rubber pad is also provided on the side of the spring plates 6 near the vaccine shake bottle to increase the stability of the clamping and protect the vaccine shake bottle.

[0057] Next, we describe the oscillation structure:

[0058] Reference Figure 2 and Figure 7A drive motor 21 is fixed to one side of the housing 1 via a frame, and its output shaft is fixedly connected to one end of a rotating rod 20 via a coupling. The outer wall of the rotating rod 20 is designed with multiple reciprocating threaded sections 22, which are threadedly connected to multiple moving blocks 23. These moving blocks 23 can slide on the bottom inner wall of the housing 1 to drive their reciprocating movement. Multiple loop-shaped retaining sleeves 24 are fixed to the bottom of the vibrating plate 2, and these retaining sleeves 24 are fitted onto the outer wall of the moving blocks 23. When the moving blocks 23 reciprocate, they drive the vibrating plate 2 to reciprocate linearly through the loop-shaped retaining sleeves 24.

[0059] Reference Figure 3 and Figure 7 To ensure the stability of the vibrating plate 2 within the housing 1, multiple first slides 17 and second slides 18 are provided on the inner walls of the two sides of the housing 1 that are far apart from each other. Multiple guide blocks 19 are fixed to both sides of the vibrating plate 2, and these guide blocks 19 can slide and engage with the first slides 17 and second slides 18. When the vibrating plate 2 is placed into the housing 1 along the first slide 17, the U-shaped retaining sleeve 24 will fit snugly onto the moving block 23. Furthermore, the bottoms of the multiple first slides 17 located on the same side are connected to the adjacent second slides 18 to facilitate the reciprocating movement of the vibrating plate 2 later.

[0060] Reference Figure 2 and Figure 8 While the rotating rod 20 drives the oscillating plate 2 to reciprocate, it can also drive the air bath heating structure. The specific implementation of the water bath heating structure is as follows:

[0061] The specific implementation method of the air bath heating structure is as follows:

[0062] Reference Figure 2 and Figure 8A heating chamber 31 is located on one side of the housing 1. A mounting bracket 35 is fixed inside the heating chamber 31 by screws. A rotating shaft 36 rotatably passes through the mounting bracket 35. A fan 37 and a first bevel gear 38 are fixed to the top and bottom of the rotating shaft 36 by screws, respectively. Multiple second heating elements 34, located above the fans 37, are fixed to the inner walls of the two opposite sides of the heating chamber 31 by screws for heating the air. An air outlet pipe 32 is fixedly connected to the top of the heating chamber 31, and one end of the air outlet pipe 32 extends into the housing 1 via a flange, for injecting hot air from the heating chamber 31 into the housing 1. A rotating shaft 39, located below the first bevel gear 38, is rotatably connected inside the heating chamber 31. A second bevel gear 40 is slidably connected to the outer wall of the rotating shaft 39 via a sliding groove and a slider, and the second bevel gear 40 meshes with the first bevel gear 38. An annular groove 52 is formed on the side of the second bevel gear 40 closest to the housing 1. A return air pipe 33 is fixedly connected to one side of the housing 1 via a flange. One end of the return air pipe 33 is fixedly connected to the heating box 31 via a flange, and the connection between the return air pipe 33 and the heating box 31 is located below the fan 37.

[0063] Specifically, the rotating rod 20 of the drive motor 21 of the oscillator extends into the heating chamber 31 at one end, which is sealed and fixedly connected to one end of the rotating shaft 39 via a coupling, for driving the second bevel gear 40 and the fan 37 to rotate. When it is necessary to perform air bath heating on the shaker inside the chamber 1, the air inside the heating chamber 31 is first heated by the second heating element 34. Then, the drive motor 21 drives the rotating rod 20 to rotate, and the rotating rod 20 drives the second bevel gear 40 and the fan 37 to rotate via the rotating shaft 39. At the same time, the solenoid valves 50 on the air outlet pipe 32 and the air return pipe 33 are opened. The fan 37 injects the hot air inside the heating chamber 31 into the chamber 1 through the air outlet pipe 32, and the cold air inside the chamber 1 flows back into the heating chamber 31 through the air return pipe 33 for reheating, thus completing the air circulation and performing air bath heating on the shaker.

[0064] Reference Figure 2 The water bath structure includes a water tank 25 located at the bottom of the housing 1. Multiple first heating elements 29 are fixed to the inner bottom wall of the water tank 25 with screws for heating the water inside. A water pump 26 is also fixed to the inner bottom wall of the water tank 25 via a frame. The outlet end of the water pump 26 is connected to an outlet pipe 27 via a flange. The top end of the outlet pipe 27 extends into the housing 1 via a flange for injecting hot water into the housing 1. A return pipe 30 is connected to the bottom of the housing 1 via a flange. The bottom end of the return pipe 30 extends into the water tank 25 via a flange. Both the outlet pipe 27 and the return pipe 30 are equipped with solenoid valves 50, which allow the hot water in the housing 1 and the water tank 25 to circulate under the control of the solenoid valves 50.

[0065] Specifically, when it is necessary to heat the shaker inside the chamber 1 with a water bath, the water in the water tank 25 is first heated by the first heating element 29, and then the water pump 26 injects hot water into the chamber 1 through the outlet pipe 27. At the same time, the solenoid valve 50 on the return pipe 30 is opened to circulate the hot water in the chamber 1 and the water tank 25, thereby ensuring the stability of the water temperature.

[0066] In the design of the thermostatic oscillator, solenoid valves 50 are installed on the outer walls of the gas outlet pipe 32, the gas return pipe 33, and the liquid return pipe 30. These solenoid valves 50 can control the opening and closing of the corresponding pipes, thereby achieving precise control of gas and liquid. For example, when it is necessary to adjust the gas composition or pressure in the incubator, this can be achieved by opening or closing the corresponding solenoid valve 50.

[0067] Reference Figure 2 , Figure 8 and Figure 9 The water bath heating structure can drive a switching structure, which controls the on / off state of the air bath heating structure. The specific implementation of the switching structure is as follows:

[0068] Reference Figure 8 and Figure 9 Multiple vertical rods 42 are fixed to the bottom inner wall of the tank 1 with screws, and the vertical rods 42 are located below the rotating rod 20. A float plate 43 is slidably fitted on the outer wall of the multiple vertical rods 42, and the float plate 43 has a trapezoidal cross-section to increase the contact area with water. A connecting rod 44 is rotatably connected to the side of the float plate 43 near the heating box 31 by a pin, and a push rod 41 is rotatably connected to the top of the connecting rod 44 by a pin. One end of the push rod 41 slides through one side inner wall of the tank 1 and extends into the heating box 31, and the other end extends into the annular groove 52 and slides into the annular groove 52. The raising and lowering of the float plate 43 can control the movement of the push rod 41 and the second bevel gear 40, thereby controlling the meshing and disengagement of the second bevel gear 40 and the first bevel gear 38.

[0069] Specifically, when hot water is injected into the tank 1 through the outlet pipe 27, as the water level rises, the float plate 43 moves upward under the action of buoyancy. The float plate 43 pushes the push rod 41 and the solenoid valve 50 away from the tank 1 through the connecting rod 44. The push rod 41 drives the second bevel gear 40 to move through the annular groove 52, disengaging the second bevel gear 40 from the first bevel gear 38, thereby disengaging the fan 37 during water bathing. Conversely, when the water level in the tank 1 drops, the float plate 43 moves downward under its own weight and drives the connecting rod 44 to rotate. The connecting rod 44 pulls the solenoid valve 50 to one side through the push rod 41. At this time, the second bevel gear 40 rotates under the drive of the rotating shaft 39 and meshes with the first bevel gear 38, injecting hot air into the tank 1 for air bathing.

[0070] This temperature-controlled shaker for vaccine shake flask culture is rationally designed and compact, firmly holding the vaccine shake flask and ensuring its stability during shaking. Furthermore, by providing two heating methods, users can choose the appropriate method to meet different culture requirements.

[0071] Example 2: Reference Figure 10 and Figure 11 An improvement upon Embodiment 1 is made as follows: The oscillating plate 2 has multiple cavities 46 for accommodating and supporting the limiting rods 45. Multiple limiting rods 45 also slide through the oscillating plate 2, with one end of each limiting rod 45 passing through a corresponding cavity 46. Trapezoidal grooves 49 are provided at the top of the limiting rods 45 for engaging with the fixing posts 48.

[0072] refer to Figure 11 Meanwhile, multiple push platforms 47 slide through the top inner walls of multiple cavities 46. The tops of these push platforms 47 slide above the oscillating plate 2 and are located within multiple clamping members 3. The bottom of each push platform 47 is fixed with a fixing post 48, the bottom of which is arc-shaped and can cooperate with the trapezoidal groove 49.

[0073] refer to Figure 11 During the process of the moving block 23 driving the oscillating plate 2 to reciprocate and drive the shaking bottle to oscillate laterally, the fixed column 48 slides within the trapezoidal groove 49. Due to the shape of the trapezoidal groove 49 and the arc-shaped bottom design of the fixed column 48, the fixed column 48 is guided by the trapezoidal groove 49 to move up and down during the sliding process. This up and down reciprocating movement drives the pushing platform 47 to move together, thereby realizing the longitudinal reciprocating movement drive of the shaking bottle.

[0074] refer to Figure 10 and Figure 11 Furthermore, the two ends of the limiting rod 45 abut against the inner walls of the housing 1 on opposite sides, which limits the range of movement of the oscillating plate 2 during lateral oscillation. Simultaneously, the bottom sides of the limiting rod 45 are provided with inclined surfaces, which facilitates the placement of the oscillating plate 2 and the limiting rod 45 inside the housing 1.

[0075] With this design, when the moving block 23 drives the oscillating plate 2 to move back and forth, the shake flask will not only be subjected to lateral oscillation force, but also to longitudinal reciprocating force generated by the cooperation of the fixed column 48, the trapezoidal groove 49, and the first spring 10. This composite oscillation method can more effectively promote the growth and reproduction of cells or microorganisms in the shake flask, improving the production efficiency and quality of vaccines.

[0076] A method for using a constant-temperature shaker for vaccine shake flask culture includes the following steps:

[0077] S1. Before use, place the shake bottle containing the vaccine in the clamping component 3. The shake bottle is initially clamped by the cooperation of the vertical plate 4, the clamping plate 5 and the spring plate 6. Then, the vibration plate 2 is placed in the box 1 by the U-shaped handle 51. When placing, the guide block 19 is smoothly placed in the box 1 by the sliding cooperation of the first slide rail 17 until the guide block 19 moves into the second slide rail 18, thus completing the initial placement of the vibration plate 2 and the shake bottle.

[0078] S2. Next, the box body 1 is closed by the top cover 7, and the engagement of the hemispherical rubber strip 15 with the slot 16 can fix the top cover 7. When the top cover 7 closes the box body 1, the top of the shake bottle is inserted into the insertion hole 9 and abuts against the bottom of the pressure plate 11. The elastic force of the first spring 10 on the pressure plate 11 can cooperate with the oscillating plate 2 to vertically clamp the shake bottle. Therefore, the cooperation of the clamping component 3 with the pressure plate 11 and the oscillating plate 2 clamps and fixes the shake bottle, ensuring the stability of the shake bottle during subsequent oscillation.

[0079] S3. When it is necessary to perform air bath heating and oscillation on the shake flask, the air inside the heating chamber 31 is heated by the second heating element 34. Then, the drive motor 21 drives the rotating rod 20 to rotate. The rotating rod 20 drives the rotating shaft 39 to rotate. The float 43 moves down under its own weight and drives the connecting rod 44 to rotate. The connecting rod 44 pulls the solenoid valve 50 to one side through the push rod 41. When the rotating shaft 39 drives the second bevel gear 40 to rotate, it meshes with the first bevel gear 38. Therefore, the rotating shaft 39 drives the fan 37 to rotate through the cooperation of the second bevel gear 40 and the first bevel gear 38. Then, the solenoid valve 50 on the air outlet pipe 32 and the air return pipe 33 is opened. The fan 37 injects the hot air inside the heating chamber 31 into the chamber 1 through the air outlet pipe 32 to control the temperature inside the chamber 1. The temperature sensor 28 can detect the stability inside the chamber 1 in real time. The cold air inside the chamber 1 flows back to the heating chamber 31 through the air return pipe 33 for reheating, thus completing the air circulation and performing air bath heating on the shake flask.

[0080] S4. When the vibrating plate 2 is placed into the box 1 along the first slide 17, the back-shaped sleeve 24 fits onto the moving block 23. Therefore, during vibration, the rotating rod 20 drives the moving block 23 to move back and forth through the reciprocating thread section 22. The cooperation between the moving block 23 and the back-shaped sleeve 24 drives the vibrating plate 2 and the shaking bottle on it to vibrate back and forth. In addition, when the vibrating plate 2 drives the shaking bottle to move, the top of the shaking bottle drives the sliding plate 8 to move synchronously, ensuring the stability of the shaking bottle during the vibration process.

[0081] S5. When it is necessary to heat the shaker in a water bath, the water in the water tank 25 is heated by the first heating element 29. The water pump 26 injects hot water into the tank 1 through the outlet pipe 27. As the water level rises, the float plate 43 moves upward under the action of buoyancy. The float plate 43 pushes the push rod 41 and the solenoid valve 50 to move away from the tank 1 through the connecting rod 44, and disengages the second bevel gear 40 from the first bevel gear 38. Therefore, when the rotating rod 20 drives the oscillating plate 2 to oscillate in the later stage, the fan 37 will not run dry. When heating the shaker inside the tank 1 in a water bath, the solenoid valve 50 on the return pipe 30 is opened to circulate the hot water in the tank 1 and the water tank 25 to ensure the stability of the water temperature.

[0082] S6. During the process of the moving block 23 driving the oscillating plate 2 to reciprocate and drive the shaker bottle to oscillate laterally, the cooperation of the fixed column 48, the trapezoidal groove 49, and the first spring 10 can drive the shaker bottle to reciprocate longitudinally, thereby increasing the shaking effect.

[0083] However, as is well known to those skilled in the art, the working principles and wiring methods of the second heating element 34, solenoid valve 50, water pump 26, first heating element 29, drive motor 21 and temperature sensor 28 are commonplace and belong to conventional methods or common knowledge. They will not be described in detail here. Those skilled in the art can make any selections according to their needs or convenience.

[0084] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A constant-temperature shaker for vaccine shake flask cultivation, characterized by comprising: Including box (1), the inside of box (1) is equipped with oscillating plate (2), the top of oscillating plate (2) is equipped with multiple clamping members (3) for clamping shake flask, both sides of the top of oscillating plate (2) are fixed with U-shaped handle (51), for lifting and pulling oscillating plate (2), the bottom inner wall of box (1) is fixed with temperature sensor (28); It also includes water tank (25) and heating tank (31), the water tank (25) is fixed at the bottom of box (1), for injecting hot water into box (1) to carry out water bath heating, the heating tank (31) is fixed at the top of water tank (25), and the heating tank (31) is fixedly connected with one side of box (1), for injecting hot air into box (1) to carry out air bath heating; It also includes top cover (7) arranged at the top of box (1), for closing box (1), the bottom of top cover (7) is slidably provided with sliding plate (8) extending into box (1), and the sliding plate (8) is matched with oscillating plate (2) for clamping shake flask from vertical direction; It also includes rotating rod (20) rotating in box (1), and the rotating rod (20) is located below oscillating plate (2); Clamping structure, arranged in sliding plate (8), for clamping shake flask in all directions with oscillating plate (2) and clamping member (3); Oscillation structure, arranged on one side of box (1), for driving rotating rod (20) to rotate to complete reciprocating oscillation movement of oscillating plate (2); Water bath heating structure, arranged in water tank (25), for injecting hot water into box (1) to carry out water bath heating; The air bath heating structure is arranged in the heating box (31) and is used for injecting hot air into the box (1) for air bath heating. The oscillation structure can drive the air bath heating structure to operate through the rotating rod (20). The air bath heating structure comprises a mounting frame (35) fixed in the heating box (31). A rotating shaft (36) is rotatably arranged in the mounting frame (35). The top end and the bottom end of the rotating shaft (36) are respectively fixed with a fan (37) and a first bevel gear (38). The mutually distal inner walls of the heating box (31) are fixed with a plurality of second heating fins (34) above the fan (37) for heating air. The top of the heating box (31) is fixedly connected with an air outlet pipe (32) extending into the box (1) for injecting hot air in the heating box (31) into the box (1). The heating box (31) is rotatably connected with a rotating shaft (39) below the first bevel gear (38). The outer wall of the rotating shaft (39) is slidably connected with a second bevel gear (40) through a sliding groove and a sliding block. The second bevel gear (40) is engaged with the first bevel gear (38). The side of the second bevel gear (40) close to the box (1) is provided with an annular groove (52). One end of a gas return pipe (33) is fixedly connected with the heating box (31). The connection between the gas return pipe (33) and the heating box (31) is below the fan (37). The end of the rotating rod (20) away from the driving motor (21) is sealingly rotatably extended into the heating box (31) and fixedly connected with one end of the rotating shaft (39) for driving the second bevel gear (40) to rotate. The switching structure is arranged in the box (1) and is used for controlling the opening and closing of the air bath heating structure. The water bath heating structure can be used for driving the switching structure. The switching structure comprises a plurality of vertical rods (42) fixed to the inner wall of the bottom of the box (1). The vertical rods (42) are below the rotating rod (20). The outer walls of the vertical rods (42) are slidably sleeved with the same floating plate (43). The cross section of the floating plate (43) is trapezoidal for increasing the contact area with water. The side of the floating plate (43) close to the heating box (31) is rotatably connected with a connecting rod (44). The top end of the connecting rod (44) is rotatably connected with a push rod (41). One end of the push rod (41) is sealingly slidably penetrating through the inner wall of one side of the box (1) and sealingly slidably extending into the heating box (31). The end of the push rod (41) extends into the annular groove (52) and is slidably matched with the annular groove (52). The lifting of the floating plate (43) is used for controlling the movement of the push rod (41) and the second bevel gear (40), thereby controlling the engagement and disengagement of the second bevel gear (40) and the first bevel gear (38).

2. The constant temperature shaker for vaccine roller bottle culture according to claim 1, characterized in that, The clamping structure comprises a plurality of insertion holes (9) arranged at the bottom of the sliding plate (8), the inner wall of the top of each of the plurality of insertion holes (9) is fixed with a first spring (10), each of the plurality of insertion holes (9) is slidably connected with a pressing plate (11) abutting against the top end of the shake flask, the top of the pressing plate (11) is fixedly connected with the bottom end of the first spring (10), and the elastic force of the first spring (10) on the pressing plate (11) can clamp the shake flask placed on the oscillating plate (2) in the vertical direction. The clamping member (3) comprises a plurality of vertical plates (4) and clamping plates (5), the vertical plates (4) are fixed at the top of the oscillating plate (2), the clamping plates (5) are fixed at the top end of the vertical plates (4), a plurality of spring sheets (6) are fixed on the side of the clamping plates (5) close to the shake flask, and the plurality of spring sheets (6) on the plurality of clamping plates (5) are matched to clamp the shake flask, and the side of the spring sheet (6) close to the shake flask is provided with a rubber pad for protecting the shake flask.

3. The constant temperature shaker for vaccine roller bottle culture according to claim 1, characterized in that, The oscillating structure comprises a drive motor (21) fixed on one side of the box (1) through a rack, the output shaft of the drive motor (21) is fixedly connected with one end of a rotating rod (20) through a shaft coupling, the outer wall of the rotating rod (20) is provided with a plurality of reciprocating threaded segments (22), the rotating rod (20) is threadedly connected with a plurality of moving blocks (23) through the plurality of reciprocating threaded segments (22), and the moving blocks (23) slide on the inner wall of the bottom of the box (1) to drive the moving blocks (23) to reciprocate, the bottom of the oscillating plate (2) is fixed with a plurality of back-shaped sleeves (24), the back-shaped sleeves (24) are sleeved on the outer wall of the moving blocks (23), and the moving blocks (23) drive the oscillating plate (2) to reciprocate linearly, the two sides of the oscillating plate (2) are fixed with a plurality of guide blocks (19), the inner walls of the two sides of the box (1) away from each other are provided with a plurality of first sliding grooves (17), and the guide blocks (19) and the first sliding grooves (17) are in sliding fit, the inner walls of the two sides of the box (1) away from each other are provided with second sliding grooves (18), and the second sliding grooves (18) and the guide blocks (19) are in sliding fit, the second sliding grooves (18) are located below the first sliding grooves (17), and the bottoms of the plurality of first sliding grooves (17) on the same side are in communication with the adjacent second sliding grooves (18), so that the oscillating plate (2) can reciprocate conveniently.

4. The constant temperature shaker for vaccine roller culture according to claim 1, characterized in that, The water bath heating structure comprises a plurality of first heating sheets (29) fixed on the inner wall of the bottom of the water tank (25) to heat the water in the water tank (25), the bottom inner wall of the water tank (25) is fixed with a water pump (26) through a rack, the liquid outlet end of the water pump (26) is fixed with a liquid outlet pipe (27), the top end of the liquid outlet pipe (27) is fixedly extended into the box (1) to inject hot water into the box (1), and the bottom of the box (1) is fixedly connected with a liquid return pipe (30) in communication, the bottom end of the liquid return pipe (30) is fixedly extended into the water tank (25), and the liquid outlet pipe (27) and the liquid return pipe (30) are matched to make the hot water in the box (1) and the water tank (25) flow circularly.

5. The constant-temperature shaker for vaccine roller culture according to claim 4, characterized in that, Limiting plates (12) are fixed on both sides of the bottom of the top cover (7). The side of the two limiting plates (12) that are far apart from each other is attached to the inner wall of the corresponding side of the box (1) to increase the stability of the top cover (7) in sealing the box (1). Multiple sliding rods (13) are fixed on the side of the two limiting plates (12) that are close to each other. One end of the multiple sliding rods (13) slides into the sliding plate (8). Multiple second springs (14) are fixed between the two limiting plates (12) and the sliding plate (8). The multiple second springs (14) are respectively sleeved on the outer wall of the corresponding sliding rods (13) to reset the sliding plate (8) to the center position of the bottom of the top cover (7).

6. The constant-temperature shaker for vaccine roller culture according to claim 5, characterized in that, Both of the two limiting plates (12) are fixed with hemispherical rubber strips (15) on the side away from each other, and the inner walls of the two sides of the box (1) that are close to each other are provided with slots (16), and the hemispherical rubber strips (15) and the slots (16) are engaged with each other.

7. The constant temperature shaker for vaccine roller bottle culture according to claim 5, characterized in that, The outer walls of the air outlet pipe (32), air return pipe (33), and liquid return pipe (30) are all equipped with solenoid valves (50).

8. The constant-temperature shaker for vaccine roller culture according to claim 3, characterized in that, The oscillating plate (2) is provided with multiple cavities (46), and multiple limiting rods (45) slide through the oscillating plate (2). One end of each limiting rod (45) passes through a corresponding cavity (46). The top of each limiting rod (45) is provided with a trapezoidal groove (49). Multiple pushing platforms (47) slide through the inner wall of the top of each cavity (46). The top of each pushing platform (47) slides to the top of the oscillating plate (2), and the multiple pushing platforms (47) are respectively located on multiple clamping components (3). Inside, the bottom of each of the multiple push platforms (47) is fixed with a fixed column (48). The bottom end of the fixed column (48) is arc-shaped and the bottom end of the fixed column (48) cooperates with the trapezoidal groove (49) to drive the push platform (47) and the fixed column (48) to move up and down reciprocally. The two ends of the limiting rod (45) respectively abut against the inner walls of the two sides of the box (1) that are far apart from each other. The bottom sides of the multiple limiting rods (45) are provided with inclined surfaces to facilitate the placement of the oscillating plate (2) and the limiting rod (45) inside the box (1).

Images