Automatic monitoring system and monitoring method for leaf surface potential in crop seedling stage

Abstract

The invention discloses an automatic monitoring system and monitoring method for leaf surface potential in a crop seedling stage. The automatic monitoring system for the leaf surface potential in the crop seedling stage comprises a seedling releasing device, a monitoring device and a recording device, the seedling placing device comprises a tray and a transportation assembly, a reference electrode is arranged on the tray, and the tray can ascend and descend in the vertical direction; the monitoring device comprises a monitoring frame, a disc, a storage box and a shielding piece, and a through hole is formed in the disc; the storage box covers the through hole; the reference electrode is matched and connected with the contact plate; a signal acquisition probe is arranged on one side of the through hole; the shielding piece is rotationally connected to the disc. The recording device comprises a light assembly and an upper computer, and one end of the light assembly extends into the monitoring frame; and the upper computer is electrically connected with the signal acquisition probe. A growth space is reserved for a to-be-detected crop, so that the problem of data deviation caused by human contact is solved, and the problem that the stable acquisition of the plant leaf surface potential is severely interfered finally due to layer-by-layer superposition of multiple experiments is solved.

Description

Technical Field

[0001] The invention belongs to the technical field of crop leaf surface potential collection, and in particular relates to an automatic monitoring system and method for crop leaf surface potential at seedling stage. Background Art

[0002] Plant electrical signals are a mechanism for information transmission and communication within plants. When plants respond to external environmental stimuli, the sensed information will affect the plant's own bioelectrical activity. Changes in electrical activity will further quickly transmit the sensed information to other plant tissues in order to produce a response mechanism. Therefore, plant electrical signals are an important part of plant cell communication and a key mechanism for plants to adapt to the environment and survive.

[0003] The technology of acquiring the light-induced plant leaf surface potential is a weak multi-source signal, and its waveform is the result of the spatiotemporal superposition of the cell membrane potential of the leaf group, which makes it very difficult for researchers to extract its features. With the continuous development of deep learning technology, the network model can automatically extract features from the original data, and then better and faster solve the problem of feature extraction and classification of light-induced plant leaf surface potential, which promotes the application of plant electrical signals in agricultural engineering and provides a new idea and technical approach for the country's agricultural intelligence, crop variety improvement and other undertakings.

[0004] In the prior art, for example, the Chinese invention patent with publication number CN108508288A discloses a plant electrical signal detection device and method, which specifically discloses an insulating container, a first electrode, a second electrode, a conductive colloid, a signal amplifier and a data acquisition unit; the conductive colloid is attached to the upper surface and the lower surface of the insulating container, one end of the first electrode is arranged in the conductive colloid on the upper surface, and one end of the second electrode is arranged in the conductive colloid on the lower surface. The other end of the first electrode and the other end of the second electrode both extend through the side of the insulating container to the outside of the insulating container, and are both connected to the signal amplifier, and the signal amplifier is connected to the data acquisition unit. The electrical signal of the plant to be tested is detected without affecting the normal growth of the plant to be tested. Contact measurement is used during detection, and no damage is caused to the plant to be tested. The overall material and experimental conditions are very low cost compared to other plant electrical signal measurement devices. However, when testing multiple samples, the entire process relies heavily on manual operations, the collection efficiency is low, and it cannot meet the huge data requirements of deep learning technology. In addition, the errors caused by human factors are superimposed in each experimental link, which will eventually cause serious interference to the stable acquisition of the surface potential of plant leaves. Summary of the invention

[0005] Based on this, it is necessary to provide an automated monitoring system and method for the leaf surface potential of crop seedlings in order to address the problem that the entire process relies heavily on manual operation, has low collection efficiency, cannot meet the huge data requirements of deep learning technology, and the errors caused by human factors are superimposed in each experimental link, which will eventually cause serious interference to the stable acquisition of the surface potential of plant leaves.

[0006] To achieve the above object, the present invention adopts the following scheme: A system for automatically monitoring the leaf surface potential of crops at the seedling stage, comprising: a seedling placing device, a monitoring device and a recording device; the seedling placing device comprises a tray and a transport assembly, the tray is arranged on the transport assembly, and a plurality of reference electrodes are arranged on the tray, and the reference electrodes can be lifted and lowered in a vertical direction and enter the monitoring device; one end of the transport assembly extends into the monitoring device; the monitoring device comprises a monitoring frame, a disc, a storage box and a shielding member, the disc is arranged in the monitoring frame and located above the transport assembly, and a plurality of through holes are arranged on the disc; the storage box cover is closed on the through holes, and a recording electrode is arranged on the upper end surface of the storage box, a contact plate is arranged on the side wall of the lower end surface of the storage box, and the reference electrode can pass through the through holes in the vertical direction. The hole enters into the storage box and is connected with the contact plate; a signal acquisition probe is arranged on one side of each through hole, and the signal acquisition probe is respectively connected to the recording electrode and the contact plate, and is used to monitor the electrical signal on the surface of the crop leaves in the storage box and send out electrical information; the shielding member is rotatably connected to the disc, the vertical center line of the shielding member passes through the center of the disc, and the shielding member is used to shield the recording electrode so that it can switch between a shielded state and an unshielded state; the recording device includes a lighting component and a host computer, one end of the lighting component extends into the monitoring frame, and is used to provide illumination for the monitoring frame and is set with a preset time; the host computer is electrically connected to the signal acquisition probe, and the host computer is used to receive the electrical information sent by the signal acquisition probe.

[0007] Preferably, the host computer includes a receiving module, a first control module, a second control module and a display module. The receiving module is electrically connected to the signal acquisition probe, and is used to receive the electrical information sent by the signal acquisition probe and determine whether the number of times the voltage of the electrical information is greater than a threshold is less than 2. If so, a first instruction is issued, and otherwise, a second instruction and a third instruction are issued; the first control module is electrically connected to the reference electrode and the receiving module, respectively, and is used to receive the first instruction and control the reference electrode to drop for the first time; the second control module is electrically connected to the receiving module and the lighting component, respectively, and is used to receive the second instruction and control the lighting component to be turned on; the The display module is electrically connected to the receiving module for receiving the third instruction and processing and displaying it; the lighting assembly includes a timing module and a third control module, the timing module is electrically connected to the reference electrode and the second control module respectively, for responding to the control of the second control module and performing timing, and issuing a fourth instruction and a fifth instruction at the same time, the fourth instruction is sent to the reference electrode for controlling the second descent of the reference electrode, and the fifth instruction is sent to the third control module; the third control module is electrically connected to the timing module and the shielding member respectively, for responding to the timing module, receiving the fifth instruction and controlling the rotation of the shielding member.

[0008] Preferably, the recording device also includes a preamplifier and an electrical signal collector. The preamplifier is electrically connected to the signal acquisition probe and is used to receive and amplify the electrical signal emitted by the signal acquisition probe. The electrical signal collector is electrically connected to the preamplifier and the host computer respectively and is used to convert the electrical signal into data information and send it to the host computer.

[0009] Preferably, the shielding member includes a base and a plurality of shading plates, the base is rotatably arranged on the upper end surface of the disk, one end of the shading plate is connected to the base, the shading plate covers directly above the through hole when in the first position, and is located between two adjacent through holes when in the second position, and the shading plate switches back and forth between the first position and the second position driven by the base.

[0010] Preferably, the base passes through the center of the disc and is connected to the disc via a rotating shaft; a drive motor is provided at the bottom of the base, the drive motor is connected to the lower end surface of the disc via a fixing bracket, and the drive motor is electrically connected to the third control module.

[0011] Preferably, a shading net is detachably provided on the monitoring frame, and the shading net is a shielding net.

[0012] Preferably, a groove corresponding to the through hole on the disc is provided on the tray, and the groove is used to place the reference electrode.

[0013] Preferably, a telescopic rod is arranged in the groove, and a push plate is arranged at one end of the telescopic rod away from the groove, and the push plate is connected to the reference electrode to drive the reference electrode to slide up and down along the side wall of the storage box.

[0014] Preferably, a rotating motor is provided on the lower end surface of the tray, and a positioning member is provided on the upper end surface of the tray. The positioning member is electrically connected to the rotating motor, and the positioning member is used to monitor the positional relationship between the groove and the through hole and control the start and stop of the rotating motor.

[0015] A method for automatically monitoring the surface potential of leaves of crops at the seedling stage, applied to any of the above-mentioned automatic monitoring systems for the surface potential of leaves of crops at the seedling stage, comprises the following steps: Step S10. Prepare a number of crops to be tested and place them on the reference electrode on the tray; Step S20. Start the transport assembly to drive the tray to move to the bottom of the disk, and stop moving when the through hole and the reference electrode overlap in the vertical direction; Step S30: The reference electrode drives the crop to be detected to rise in the vertical direction, pass through the through hole and enter the storage box, and stops when it contacts the recording electrode on the top of the storage box; Step S40. After the top of the crop to be detected contacts the recording electrode, the reference electrode moves downward by a reserved distance and waits for the crop to be detected to grow by itself; Step S50: When the top of the crop to be detected contacts the recording electrode again, the light assembly is turned on and timing is performed, and the shielding member rotates at the same time; Step S60: The signal acquisition probe monitors and sends the data to the host computer for processing and display; Step S70. After the timing of the light assembly ends, the light assembly is turned off, the shielding member stops rotating, and the reference electrode is lowered back onto the tray; Step S80: The transport assembly moves to drive another tray to move directly below the disc, and steps S20 to S80 are repeated.

[0016] The technical solution adopted in this application can achieve the following beneficial effects: 1. By rising and falling the reference electrode and leaving growth space for the crops to be tested, the problem of data deviation caused by human contact during monitoring of the crops to be tested is solved, and the problem of multiple experiments stacked on top of each other will eventually cause serious interference to the stable acquisition of the surface potential of plant leaves.

[0017] 2. Through the setting of pallets and transport components, the placement of crops to be tested is more mechanized, and several crops to be tested can be placed on the pallet, and multiple crops can be monitored at the same time, solving the problem of low efficiency of single monitoring and inability to meet the huge data volume requirements of deep learning technology.

[0018] 3. Through mechanical control, ensure that the light-induced time is the same, create better and identical monitoring conditions, and make the monitoring data more accurate.

[0019] 4. The storage box is used instead of the clip to solve the problem of monitoring errors caused by human factors during installation. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is an overall schematic diagram of the automatic monitoring system for leaf surface potential of crops in the seedling stage disclosed in the embodiments of the present application.

[0021] Figure 2 A perspective view of the automatic monitoring system for leaf surface potential of crops in the seedling stage disclosed in an embodiment of the present application.

[0022] Figure 3 It is a side view of the automatic monitoring system for leaf surface potential of crops in the seedling stage disclosed in an embodiment of the present application.

[0023] Figure 4 It is a partial bottom view of the automatic monitoring system for leaf surface potential of crops in the seedling stage disclosed in an embodiment of the present application.

[0024] Figure 5 This is a bottom view of the disc of the automatic monitoring system for leaf surface potential of crops in the seedling stage disclosed in an embodiment of the present application.

[0025] Figure 6 A partial top view of the automatic monitoring system for leaf surface potential of crops in the seedling stage disclosed in an embodiment of the present application.

[0026] Figure 7 This is an enlarged view of part A of the automatic monitoring system for leaf surface potential of crops in the seedling stage disclosed in the embodiment of the present application.

[0027] Figure 8 This is a schematic diagram of the first position of the sunshade of the automatic monitoring system for leaf surface potential of crops in the seedling stage disclosed in an embodiment of the present application.

[0028] Fig. 9 This is a schematic diagram of the second position of the sunshade of the automatic monitoring system for leaf surface potential of crops in the seedling stage disclosed in an embodiment of the present application.

[0029] Fig.10 This is a control flow chart of the method for automatically monitoring leaf surface potential of crops at the seedling stage disclosed in an embodiment of the present application.

[0030] Among them: seedling release device 100, transportation component 110, positioning equipment 111, first positioning end 1111, detection end 1112, second positioning end 1113, tray 120, telescopic rod 121, reference electrode 122, groove 123, push plate 124, rotating motor 125, positioning member 126, laser positioning instrument 1261, positioning plate 1262, monitoring device 200, monitoring frame 210, shading net 211, disc 220, through hole 221, signal acquisition probe 222, storage box 230, recording electrode 231, contact plate 232, shielding member 240, base 241, shading plate 242, drive motor 243, recording device 300, lighting component 310, host computer 320, preamplifier 330, electrical signal collector 340. DETAILED DESCRIPTION

[0031] In order to facilitate the understanding of the present application, the present application will be described more fully below with reference to the relevant drawings. The preferred embodiments of the present application are given in the drawings. However, the present application can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present application more thoroughly and comprehensively understood.

[0032] It should be noted that when a device is considered to be "connected" to another device, it may be directly connected to the other device or there may be an intermediate device at the same time. The terms "inside", "top", "upper", "lower", "upper", "lower" and similar expressions used herein are for illustrative purposes only and do not represent the only implementation method.

[0033] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0034] See also Figures 1 to 9The present application provides an automatic monitoring system for leaf surface potential of crops in seedling stage, comprising: a seedling placing device 100, a monitoring device 200 and a recording device 300; the seedling placing device 100 comprises a tray 120 and a transport component 110, the tray 120 is arranged on the transport component 110, and a plurality of reference electrodes 122 are arranged on the tray 120, and the reference electrodes 122 can be lifted and lowered in the vertical direction and enter the monitoring device 200; one end of the transport component 110 extends into the monitoring device 200; the monitoring device 200 comprises a monitoring frame 210, a disc 220, a storage box 230 and a shielding member 240, the disc 220 is arranged in the monitoring frame 210, and is located at the transport component 110. Above the component 110, the disk 220 is provided with a plurality of through holes 221; the storage box 230 is covered on the through holes 221, and the upper end surface of the storage box 230 is provided with a recording electrode 231, and the side wall of the lower end surface of the storage box 230 is provided with a contact plate 232, and the reference electrode 122 can penetrate the through hole 221 in the vertical direction to enter the storage box 230 and be connected with the contact plate 232; a signal acquisition probe 222 is provided on one side of each of the through holes 221, and the signal acquisition probe 222 is respectively connected to the recording electrode 231 and the contact plate 232, and is used to monitor the electrical signal on the surface of the crop leaves in the storage box 230 and send out electrical information; the shielding member 2 40 is rotatably connected to the disk 220, the center line of the shielding member 240 in the vertical direction passes through the center of the disk 220, and the shielding member 240 is used to shield the recording electrode 231 so that it can switch between a shielded state and an unshielded state; the recording device 300 includes a lighting component 310 and a host computer 320, one end of the lighting component 310 extends into the monitoring frame 210, and is used to provide illumination for the monitoring frame 210 and is set with a preset time; the host computer 320 is electrically connected to the signal acquisition probe 222, and the host computer 320 is used to receive the electrical information sent by the signal acquisition probe 222; the host computer 320 includes a receiving module, a first control module, a second control module and a display module The receiving module is electrically connected to the signal acquisition probe 222, and is used to receive the electrical information sent by the signal acquisition probe 222 and determine whether the number of times the voltage of the electrical information is greater than the threshold is less than 2. If so, a first instruction is issued, and if not, a second instruction and a third instruction are issued; the first control module is electrically connected to the reference electrode 122 and the receiving module, respectively, and is used to receive the first instruction and control the reference electrode 122 to drop for the first time; the second control module is electrically connected to the receiving module and the lighting component 310, respectively, and is used to receive the second instruction and control the lighting component 310 to turn on; the display module is electrically connected to the receiving module, and is used to receive the third instruction and display it;The lighting assembly 310 includes a timing module and a third control module. The timing module is electrically connected to the reference electrode 122 and the second control module, respectively, and is used to respond to the control of the second control module and perform timing, and simultaneously issue a fourth instruction and a fifth instruction. The fourth instruction is sent to the reference electrode 122 to control the second descent of the reference electrode 122, and the fifth instruction is sent to the third control module; the third control module is electrically connected to the timing module and the shielding member 240, respectively, and is used to respond to the timing module and receive the fifth instruction to control the rotation of the shielding member 240. ;

[0035] Specifically, the seedling release device 100 is divided into a detection area, a monitoring area and a sample processing area. The transport component 110 uses but is not limited to equipment with a transport function such as a conveyor belt and a loader. The starting end of the transport component 110 is set in the detection area, and the end surrounds the monitoring area and the sample processing area, and is connected to the starting end to form a ring; at least two trays 120 are set (the present application takes two as an example), and both are circular, and the tray 120 is provided with a plurality of telescopic cylinders, hydraulic rods and other devices that can rise independently (the rising and falling switches are electrically connected to the reference electrode 122, and the reference electrode 122 is electrically connected to the first control module; or the rising and falling switches are directly electrically connected to the first control module), and the layout spacing is the same as the layout spacing of the through holes 221 on the disc 220 and the positions correspond one to one, and A reference electrode 122 is arranged on it; the tray 120 is arranged on the transport component 110 in a detachable connection manner, and a spacing is arranged between the two trays 120 (the spacing is adjusted according to the length of the transport component 110, and the optimal spacing is half of the length of the transport component 110. If there are three trays 120, the spacing is one-third of the length of the transport component 110; and the same can be said for the following); a number of crops to be inspected are placed in the inspection area, and the crops to be inspected are placed on a number of independent rising devices on the tray 120 by means of a robot arm, manual labor, loading machinery, etc.; the sample processing area also unloads the sample crops that have been monitored on the number of independent rising devices on the tray 120 by means of a robot arm, manual labor, and unloading machinery, and processes them (unified recycling, on-site throwing, etc.).

[0036] The monitoring device 200 is arranged in the monitoring area, the monitoring frame 210 is placed on the ground of the monitoring area, and the transport component 110 passes through the monitoring frame 210, and the monitoring frame 210 is made of a material with a shielding function. For example, a Faraday shielding frame is closed by a black blackout cloth during monitoring, and the best choice is a Faraday shielding net; the disc 220 is arranged in the monitoring frame 210, and is fixed by means of support rods, etc., and the disc 220 is located directly above the transport component 110 in the monitoring area, and a plurality of through holes 221 are evenly distributed on the disc 220, and the spacing between two adjacent through holes 221 is not less than the diameter of the through hole 221 (the present application takes four through holes 221 as an example), and the number of storage boxes 230 arranged corresponds to the number of through holes 221 (in order to ensure the growth environment of the crops to be detected, storage boxes can be provided with through holes 221). A vent hole is provided on the side wall of the disk 230), and a circular transparent box body is adopted, and a recording electrode 231 is provided at the upper end and the lower end is opened, and it covers the through hole 221, and a contact plate 232 is provided on the side wall of the lower end surface of the storage box 230, and the length of the contact plate 232 is adjusted according to the height of the crop to be detected; a circular hole is provided at the center of the disk 220, one end of the shielding member 240 passes through the circular hole and adopts the transmission cooperation with the transportation component 110, mechanical drive, etc., so that it can rotate along the center of the disk 220, and the shielding member 240 can adopt a device with a rice support, a bamboo dragonfly, etc.

[0037] The disc 220 is provided with signal acquisition probes 222 having the same number as the through holes 221. The positions of the signal acquisition probes 222 correspond to the positions of the through holes 221 one by one and are located between the outer ring of the disc 220 and the through holes 221. The two ends of the signal acquisition probes 222 are respectively connected to the recording electrode 231 and the contact plate 232 on the top of the storage box 230. When the reference electrode 122 of the tray 120 capable of an independent rising device rises into the storage box 230 and is connected to the contact plate 232, the signal acquisition probes 222 monitor the electrical signals on the surface of the crop leaves to be detected through the reference electrode 122 and the recording electrode 231 and send electrical information.

[0038] The lighting assembly 310 includes a halogen lamp and a number of gooseneck optical fibers, one end of the gooseneck optical fiber is connected to the halogen lamp, and the other end passes through the top of the monitoring frame 210, extends into the monitoring frame 210 and is located above the shielding member 240. A timing device can be set at the switch of the halogen lamp. By setting the time, the illumination time can be the same, thereby solving the problem of different light-induced durations caused by different illumination durations. The host computer 320 uses electronic equipment such as computers and main servers to make judgments, process statistics, etc. by receiving the electrical information sent by the signal acquisition probe 222, and display it intuitively.

[0039] Furthermore, the crop to be inspected is manually placed on the reference electrode 122 on the tray 120 in the area to be inspected, and the position of the reference electrode 122 is adjusted so that it corresponds to and coincides with the position of the through hole 221 when it rises, and the transport assembly 110 is turned on. When the tray 120 is transported to the position directly below the disc 220 and the horizontal projection of the through hole 221 coincides with the reference electrode 122, the rotation of the transport assembly 110 is stopped, and the device capable of rising on the tray 120 is manually turned on, so that the reference electrode 122 is driven to pass through the corresponding through hole 221 in the vertical direction into the corresponding storage box 230 and is connected to the corresponding contact plate 232; the reference electrode 122 continues to Move upward so that the top of the crop to be detected contacts the recording electrode 231 first. At this time, the signal acquisition probe 222 detects that the voltage of the electrical information is greater than the threshold value (the threshold value in this application is 5mV), and sends it to the receiving module of the host computer 320. The receiving module determines whether the electrical information sent by the signal acquisition probe 222 is the first time in this batch of monitoring (this time is the first time in this batch of monitoring). If so, it sends a first instruction to the first control module to control the reference electrode 122 to move down for the first time, move down a reserved distance (the reserved distance is used to allow the crop to be detected to grow by itself, 5 mm to 10 mm), and then wait for the object to be detected to grow; when its top After the end contacts the recording electrode 231, the signal acquisition probe 222 detects that the voltage of the electrical information is greater than the threshold value, and sends it to the receiving module of the host computer 320. The receiving module determines that in this batch of monitoring, the electrical information sent by the signal acquisition probe 222 is the second time (this time is the second time of this batch of monitoring), so it sends a second instruction (an instruction to turn on the timing module) to the lighting component 310 to turn on the timing module, and at the same time controls the shielding member 240 to rotate, so that the crop to be detected is light-induced by flickering, and the signal acquisition probe 222 monitors it and sends the electrical information to the receiving module. The receiving module sends a third instruction for processing, classification, summary and packaging, and then sends The data are sent to the display module for display; when the timing module in the lighting assembly 310 ends, a fifth instruction is issued to the third control module to stop the shielding member 240 from rotating, and a fourth instruction is issued to the reference electrode 122 to make the reference electrode 122 drop back to the starting position for the second time (the reference electrode 122 is in the position when it was raised), thereby completing the monitoring of this batch of samples; the transport assembly 110 is turned on, and the tray 120 is transported to the sample processing area, while the other tray 120 rotates to the bottom of the disc 220 (when one tray 120 is being monitored, the other tray 120 is unloading the sample and placing the crop to be tested), and the above steps are repeated to complete the continuous monitoring.

[0040] The present application adopts a technical solution of an automatic monitoring system for the surface potential of crop leaves in the seedling stage to achieve the following beneficial effects: 1. By rising and falling the reference electrode 122 and leaving growth space for the crops to be detected, the problem of data deviation caused by human contact during monitoring of the crops to be detected is solved, and the problem of multiple experiments stacked on top of each other will eventually cause serious interference to the stable acquisition of the potential on the surface of the plant leaves is solved.

[0041] 2. By setting up the tray 120 and the transport component 110, the placement of the crops to be inspected is more mechanized, and several crops to be inspected can be placed on the tray 120, and multiple crops can be monitored at the same time, solving the problem of low efficiency of single monitoring and inability to meet the huge data volume requirements of deep learning technology.

[0042] 3. Through mechanical control, ensure that the light-induced time is the same, create better and identical monitoring conditions, and make the monitoring data more accurate.

[0043] 4. The storage box 230 is used instead of the clip to solve the problem of monitoring errors caused by human factors during installation.

[0044] Based on the above scheme, the recording device 300 also includes a preamplifier 330 and an electrical signal collector 340. The preamplifier 330 is electrically connected to the signal acquisition probe 222 for receiving and amplifying the electrical signal emitted by the signal acquisition probe 222. The electrical signal collector 340 is electrically connected to the preamplifier 330 and the host computer 320 respectively for converting the electrical signal into data information and sending it to the host computer 320.

[0045] Specifically, a support platform is provided on a side of the monitoring frame 210 away from the transport component 110, and the support platform is in a stepped shape. Preamplifiers 330 having the same number as the through holes 221 are provided on the bottom step of the support platform, and the input end of each preamplifier 330 is electrically connected to the output end of the signal acquisition probe 222, and the electrical information emitted by the signal acquisition probe 222 is amplified by the preamplifier 330; an electrical signal collector 340 is provided on the middle step, and the input end of the electrical signal collector 340 is connected to the output end of the preamplifier 330 if any, and the output end of the electrical signal collector 340 is connected to the input end of the host computer 320; the host computer 320 is provided at the highest point of the support platform, and the installation position of the device is improved by providing the support platform, so that the connection with the corresponding components is more convenient; the electrical information emitted by the signal acquisition probe 222 is amplified by the preamplifier 330, and then the electrical signal collector 340 receives, summarizes and packages it uniformly, and sends the packaged data to the host computer 320 for display, so that the operation is more convenient, the captured electrical signals are clearer, and the data are more accurate.

[0046] Based on the above scheme, the shielding member 240 includes a base 241 and a plurality of shading plates 242. The base 241 is rotatably arranged on the upper end surface of the disk 220. One end of the shading plate 242 is connected to the base 241. When the shading plate 242 is in the first position, it covers directly above the through hole 221. When the shading plate 242 is in the second position, it is located between two adjacent through holes 221. Driven by the base 241, the shading plate 242 switches back and forth between the first position and the second position.

[0047] Furthermore, in order to achieve stability, the base 241 passes through the center of the disc 220 and is connected to the disc 220 via a rotating shaft; a drive motor 243 is provided at the bottom of the base 241, and the drive motor 243 is connected to the lower end surface of the disc 220 via a fixing frame, and the drive motor 243 is electrically connected to the third control module.

[0048] Specifically, the base 241 adopts a circular plate at the bottom, and the upper end surface of the circular plate is connected to a fixing plate with the same number as the through holes 221, and the positions correspond one to one, and the other end of the fixing plate is fixedly connected to a light shielding plate 242, which is circular and has the same size as the recording electrode 231; the light shielding plate 242 is in the second position when the driving motor 243 has not yet started to rotate, and when the third control module receives the fifth instruction, the driving motor 243 responds and starts to rotate, so that the light shielding plate 242 switches from the first position to the second position and reciprocates; When the timing module finishes timing, the third control module controls the driving motor 243 to stop again, and the driving motor 243 drives the shading plate 242 to slowly stop at the second position; the shading plate 242 covers the recording electrode 231, so that the recording electrode 231 is switched between the shielded state and the unshielded state, thereby realizing flickering light induction, which is more convenient to operate, and through the control of the timing module and the third control module, the light induction time is more accurate, thereby solving the problem of different light induction times caused by human control, resulting in errors in monitoring data.

[0049] In the above scheme, in order to improve the accuracy of the data, the monitoring frame 210 is provided with a detachable shading net 211, and the shading net 211 is a shielding net. The shading net 211 is arranged on the peripheral side of the monitoring frame 210 by means of buckles, bolts, etc., and the top of the monitoring frame 210 is fixedly connected with the shading net 211. The outer side of the disk 220 is also sealed by the shading net 211 to prevent natural light from entering the monitoring frame 210 from below. The shading net 211 is a light-proof Faraday shielding net. When no test is performed, the shading net 211 is removed and stored; when the test is performed, the shading net 211 on the peripheral side is installed, so that the inside of the monitoring frame 210 is in a light-proof state, so as to solve the problem that the natural light affects the light induction and causes the data error.

[0050] To achieve the purpose of positioning, in the above scheme, the tray 120 is provided with a groove 123 corresponding to the through hole 221 on the disk 220, and the groove 123 is used to place the reference electrode 122. A telescopic rod 121 is provided in the groove 123, and a push plate 124 is provided at one end of the telescopic rod 121 away from the groove 123. The push plate 124 is connected to the reference electrode 122 and is used to drive the reference electrode 122 to slide up and down along the side wall of the storage box 230.

[0051] Furthermore, a rotating motor 125 is provided on the lower end surface of the tray 120, and a positioning member 126 is provided on the upper end surface of the tray 120. The positioning member 126 is electrically connected to the rotating motor 125, and the positioning member 126 is used to monitor the positional relationship between the groove 123 and the through hole 221, and control the start and stop of the rotating motor 125.

[0052] Specifically, a sleeve is detachably provided on the transport component 110, and a rotating motor 125 is provided in the sleeve. The shaft extension end of the rotating motor 125 is connected to the lower end surface of the tray 120. A plurality of grooves 123 are provided on the tray 120. The number of the grooves 123 is the same as the number of the through holes 221, and the positions correspond one to one. The positioning member 126 includes a laser positioning device 1261 and a positioning plate 1262. The positioning plate 1262 is provided on the lower end surface of the disk 220 and is located on both sides of the through hole 221. The laser positioning device 1261 is provided on the lower end surface of the disk 220 and is located on both sides of the through hole 221. 61 is arranged on both sides of the groove 123 and is at the same position as the positioning plate 1262. The laser locator 1261 is connected to the rotating motor 125 through a controller. The controller is used to receive parameter information of the laser locator 1261 and the positioning plate 1262, and control the rotating motor 125 to rotate, thereby adjusting the position of the laser locator 1261 until the projection of the groove 123 and the through hole 221 in the vertical direction coincides (the laser locator 1261 is located directly below the positioning plate 1262).

[0053] A telescopic rod 121 is arranged in the groove 123. When the telescopic rod 121 is in a retracted state, the telescopic rod 121 is retracted in the groove 123, and the top of the telescopic rod 121 is flush with the upper end surface of the disc 220. A push plate 124 is arranged at the other end of the telescopic rod 121, and a columnar box is arranged on the push plate 124. The bottom of the columnar box is a reference electrode 122, and the top of the columnar box is exposed and its diameter is smaller than the diameter of the storage box 230. The columnar box is sealed with the reference electrode 122. The extension and retraction of the telescopic rod 121 is electrically connected to the first control module, and the first descent of the telescopic rod 121 is realized by receiving the first instruction.

[0054] In order to realize the purpose of automatic extension and retraction of the telescopic rod 121, the controller is electrically connected to the laser locator 1261 of the positioning member 126 to determine whether the laser locator 1261 and the positioning plate 1262 overlap. If they do not overlap, a command for the rotating motor 125 to rotate is issued. If they overlap, a command for the telescopic rod 121 to rise and a command for the rotating motor 125 to stop rotating are issued at the same time. By setting the controller so that it is electrically connected to the rotating motor 125 and the telescopic rod 121 respectively, the position of the groove 123 is adjusted first so that the reference electrode 122 is located directly below the through hole 221, and then the telescopic rod 121 is extended and retracted upward so that the reference electrode 122 passes through the through hole 221 in the vertical direction and enters the storage box 230, and the top of the crop to be detected is in contact with the recording electrode 231; thereby, the purpose of automatic extension and retraction of the telescopic rod 121 is realized.

[0055] In order to facilitate the operation and realize the purpose of automatic stopping of the telescopic rod 121, the controller is electrically connected to the signal acquisition probe 222. When the electrical information of the signal acquisition probe 222 is sent to the controller, the controller issues a command for the telescopic rod 121 to stop rising, thereby realizing the purpose of automatic stopping of the telescopic rod 121.

[0056] Furthermore, in order to achieve the purpose of full automation, the transport component 110 includes a conveyor belt and a transport motor. The transport motor is connected to the conveyor belt through a main controller, and a positioning device 111 that is the same as the positioning piece 126 on the pallet 120 is arranged on the conveyor belt. The positioning device 111 is electrically connected to the main controller, and the positioning end of the positioning device 111 is arranged on two opposite sides of the disc 220. The detection end 1112 of the positioning device 111 is arranged on both sides of the pallet 120, and the spacing between the two positioning ends is the same as the spacing between the two detection ends 1112. When the detection end 1112 and the fixed end overlap in the vertical direction, the center line of the pallet 120 and the disc 220 in the vertical direction overlap; the main controller obtains parameter information of the positioning end and the detection end 1112 of the positioning device 111 (if there are two pallets 120, it is the first pallet 120 and the second pallet 120, The detection ends 1112 of the positioning device 111 on the transmission belt are correspondingly named as the first detection end 1112 and the second detection end 1112; when the first detection end 1112 coincides with the positioning end, the first detection end 1112 is temporarily closed, and when the detection is turned on again, the second detection end 1112 is opened, thereby realizing the interchange of the first detection end 1112 and the second detection end 1112, so that the first pallet 120 and the second pallet 120 can form a circular monitoring), and judging whether the positioning end and the detection end 1112 of the positioning device 111 coincide with each other, if not, the transport motor is controlled to rotate to drive the conveyor belt to move along a predetermined rule, if coincident, the transport motor is controlled to stop to stop the conveyor belt; the problem of deviation in the stopping position caused by lack of proficiency in manually controlling the transport component 110 is solved, thereby solving the problem of difficulty in aligning the groove 123 and the through hole 221 due to position deviation.

[0057] For overall unification, the main controller, the controller and the receiving module of the host computer 320 are electrically connected to form a unified control, so that the operation is more convenient and unified.

[0058] See also Fig.10 , provides a method for automatically monitoring the surface potential of leaves of crops at the seedling stage, which is applied to any of the automatic monitoring systems for the surface potential of leaves of crops at the seedling stage, and comprises the following steps: Step S10. Prepare a plurality of crops to be tested and place them on the reference electrode 122 on the tray 120; Step S20. Open the transport assembly 110 to drive the tray 120 to move to the bottom of the disk 220, and stop moving when the through hole 221 and the reference electrode 122 overlap in the vertical direction; Step S30. The reference electrode 122 drives the crop to be detected to rise in the vertical direction, pass through the through hole 221 and enter the storage box 230, and stops when it contacts the recording electrode 231 on the top of the storage box 230; Step S40. After the top of the crop to be detected contacts the recording electrode 231, the reference electrode 122 moves downward by a reserved distance and waits for the crop to be detected to grow by itself; Step S50. When the top of the crop to be detected contacts the recording electrode 231 again, the light assembly 310 is turned on and timing is performed, and the shielding member 240 rotates at the same time; Step S60. The signal acquisition probe 222 performs monitoring and sends the data to the host computer 320 for processing and display; Step S70. After the timing of the light assembly 310 ends, the light assembly 310 is turned off, the shielding member 240 stops rotating, and the reference electrode 122 descends back onto the tray 120; Step S80: The transport assembly 110 moves to drive another tray 120 to move to the position directly below the disc 220, and steps S20 to S80 are repeated.

[0059] The specific control process is as follows: In the initial state, the two trays 120 are respectively located between the monitoring area and the seedling area, and the reference electrodes 122 of the trays 120 are placed with crops to be detected, and the first positioning end 1111 and the second positioning end 1113 are both in the closed state; the entire crop seedling leaf surface potential automatic monitoring system is powered on and the host computer 320 is turned on, and the host computer 320 starts to run. First, the receiving module sends an instruction to turn on the main controller, and the main controller responds to the instruction to turn on and receive parameter information of the first positioning end 1111 and the detection end 1112 of the positioning device 111, and determines whether the first positioning end 1111 of the positioning device 111 coincides with the detection end 1112. If not, the transport motor is controlled to rotate, driving the conveyor belt to move along a predetermined rule until the positioning end of the first positioning device 111 coincides with the detection end 1112, and a conveyor belt stop instruction is issued and the first positioning end 1111 is closed, and a completion and termination instruction is sent to the receiving module.

[0060] The receiving module responds to the completion and termination instruction issued by the main controller, and sends an opening instruction to the controller. The controller responds to the opening instruction issued by the receiving module, obtains the parameter information of the laser locator 1261 and the positioning plate 1262, and determines whether the laser locator 1261 and the positioning plate 1262 overlap. If not, an instruction to rotate the rotating motor 125 is issued, so that the rotating motor 125 drives the tray 120 to rotate until the laser locator 1261 and the positioning plate 1262 overlap. The controller obtains the parameter information of the laser locator 1261 and the positioning plate 1262. If the laser locator 1261 and the positioning plate 1262 overlap, an instruction to stop the rotation of the rotating motor 125 and an instruction to raise the telescopic rod 121 are issued; the telescopic rod 121 rises in response to the instruction; the controller determines whether the signal acquisition probe 222 sends the electrical information of the electrical signal on the surface of the crop leaf. If so, an instruction to stop the telescopic rod 121 from rising is issued, and a completion and termination instruction is sent to the receiving module.

[0061] The receiving module responds to the completion and termination instructions issued by the controller, and obtains the electrical information of the crop leaf surface electrical signal issued by the signal acquisition probe 222, and determines whether the number of times the voltage of the electrical information is greater than the threshold is less than 2; if so, a first instruction to control the reference electrode 122 to descend for the first time is issued to the first control module, and the first control module responds to the first instruction and sends an instruction to the controller to control the telescopic rod 121 to move downward a preset distance, which is responded and executed by the controller; after the execution is completed, the receiving module waits, and at this time, the receiving module continues to obtain the electrical information of the crop leaf surface electrical signal issued by the signal acquisition probe 222, and determines whether the number of times the voltage of the electrical information is greater than the threshold is less than 2, if not, the receiving module The second instruction and the third instruction are respectively sent to the second control module and the display module; the second control module responds to the second instruction sent by the receiving module, turns on the timing module, and at the same time the timing module responds to the second instruction and sends a fifth instruction to the third control module to turn on the shielding member 240, and the third control module controls the shielding member 240 to rotate in response to the fifth instruction; at the same time, the display module responds to the third instruction sent by the receiving module, processes and displays the electrical information sent by the acquired signal acquisition probe 222; after the timing module receives the timing, it sends a completion and termination instruction, an instruction for the second descent of the telescopic rod 121, and an instruction for stopping the rotation of the shielding member 240 to the receiving module, the controller, and the third control module at the same time.

[0062] The third control module responds to the instruction of the timing module to stop the rotation of the shielding member 240, so that the shielding member 240 returns to the second position; the controller responds to the second descending instruction issued by the timing module, so that the telescopic rod 121 returns to the groove 123 and stops; the receiving module responds to the completion and termination instruction issued by the timing module, and sends an instruction to turn on the main controller. The main controller responds to the instruction to turn on and receive the parameter information of the second positioning end 1113 and the detection end 1112 of the positioning device 111, and determines whether the second positioning end 1113 of the positioning device 111 coincides with the detection end 1112. If not, the transport motor is controlled to rotate, driving the conveyor belt to move along a predetermined rule until the positioning end of the second positioning device 111 coincides with the detection end 1112, and sends an instruction to stop the conveyor belt and close the second positioning end 1113, and at the same time sends an instruction to complete and terminate to the receiving module.

[0063] Repeat the above steps to realize the automatic monitoring of the continuous cycle of leaf surface potential of crops in the seedling stage.

[0064] The present application adopts a technical solution of an automated monitoring method for leaf surface potential of crop seedlings to achieve the following beneficial effects: 1. By automatically controlling the start and stop of the transport component 110, the problem of deviation in the stop position caused by lack of manual control of the transport component 110 is solved, thereby solving the problem of difficulty in aligning the groove 123 and the through hole 221 due to position deviation.

[0065] 2. By automatically controlling the rise and fall of the telescopic rod 121, the phenomenon of data monitoring deviation caused by human operation causing contact with the crop to be detected is avoided, thereby solving the problem that multiple experiments are superimposed on each other, which will eventually cause serious interference to the stable acquisition of the surface potential of the plant leaves.

[0066] 3. By leaving growth space, the crops to be tested have a period of time to grow on their own, solving the problem of data deviation caused by human contact when monitoring the crops to be tested.

[0067] 4. By mechanically controlling the time of light induction, the experiment is ensured to be under the same lighting conditions, thus solving the problem of data deviation caused by different monitoring conditions each time due to human control.

[0068] The above-described embodiments only express the way of equipment layout of the present application, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent application; it should be pointed out that for ordinary technicians in this field, several adjustments and improvements can be made without departing from the concept of the present application, which all belong to the scope of protection of the present application; therefore, the scope of protection of the patent of this application shall be based on the attached claims.

Claims

1. An automatic monitoring system for leaf surface potential of crops in seedling stage, characterized in that: include: Seedling placing device, monitoring device and recording device; The seedling placing device comprises a tray and a transport assembly, wherein the tray is arranged on the transport assembly, and a plurality of reference electrodes are arranged on the tray, and the reference electrodes can be lifted and lowered in a vertical direction and enter the monitoring device; one end of the transport assembly extends into the monitoring device; The monitoring device comprises a monitoring frame, a disc, a storage box and a shielding member, wherein the disc is arranged in the monitoring frame and is located above the transport assembly, and a plurality of through holes are arranged on the disc; the storage box cover is closed on the through holes, and a recording electrode is arranged on the upper end surface of the storage box, and a contact plate is arranged on the side wall of the lower end surface of the storage box, and the reference electrode can pass through the through hole in the vertical direction to enter the storage box and be connected with the contact plate; a signal acquisition probe is arranged on one side of each of the through holes, and the signal acquisition probe is respectively connected to the recording electrode and the contact plate, and is used to monitor the electrical signal on the surface of the crop leaves in the storage box and send out electrical information; the shielding member is rotatably connected to the disc, and the vertical center line of the shielding member passes through the center of the disc, and the shielding member is used to shield the recording electrode so that it can be switched between a shielded state and an unshielded state; and The recording device includes a lighting component and a host computer. One end of the lighting component extends into the monitoring frame to provide lighting for the monitoring frame and is set with a preset time. The host computer is electrically connected to the signal acquisition probe and is used to receive electrical information sent by the signal acquisition probe.

2. The automatic monitoring system for leaf surface potential of crops at seedling stage according to claim 1, characterized in that: The host computer includes a receiving module, a first control module, a second control module and a display module. The receiving module is electrically connected to the signal acquisition probe, and is used to receive the electrical information sent by the signal acquisition probe and determine whether the number of times the voltage of the electrical information is greater than a threshold is less than 2. If so, a first instruction is issued, and if not, a second instruction and a third instruction are issued; the first control module is electrically connected to the reference electrode and the receiving module, respectively, and is used to receive the first instruction and control the reference electrode to drop for the first time; the second control module is electrically connected to the receiving module and the lighting component, respectively, and is used to receive the second instruction and control the lighting component to be turned on; the display module is electrically connected to the receiving module, and is used to receive the third instruction and process and display it; The lighting assembly includes a timing module and a third control module. The timing module is electrically connected to the reference electrode and the second control module, respectively, and is used to respond to the control of the second control module and perform timing, and simultaneously issue a fourth instruction and a fifth instruction. The fourth instruction is sent to the reference electrode to control the second descent of the reference electrode, and the fifth instruction is sent to the third control module; the third control module is electrically connected to the timing module and the shielding member, respectively, and is used to respond to the timing module and receive the fifth instruction to control the rotation of the shielding member.

3. The automatic monitoring system for leaf surface potential of crops at seedling stage according to claim 1, characterized in that: The recording device also includes a preamplifier and an electrical signal collector. The preamplifier is electrically connected to the signal acquisition probe and is used to receive and amplify the electrical signal emitted by the signal acquisition probe. The electrical signal collector is electrically connected to the preamplifier and the host computer respectively and is used to convert the electrical signal into data information and send it to the host computer.

4. The automatic monitoring system for leaf surface potential of crops at seedling stage according to claim 2, characterized in that: The shielding member includes a base and a plurality of shading plates, wherein the base is rotatably arranged on the upper end surface of the disk, one end of the shading plate is connected to the base, and the shading plate covers directly above the through hole when in the first position, and is located between two adjacent through holes when in the second position. The shading plate switches back and forth between the first position and the second position driven by the base.

5. The automatic monitoring system for leaf surface potential of crops at seedling stage according to claim 4, characterized in that: The base passes through the center of the disc and is connected to the disc via a rotating shaft; a driving motor is arranged at the bottom of the base, the driving motor is connected to the lower end surface of the disc via a fixing frame, and the driving motor is electrically connected to the third control module.

6. The automatic monitoring system for leaf surface potential of crops at seedling stage according to claim 1, characterized in that: The monitoring frame is detachably provided with a shading net, and the shading net is a shielding net.

7. The automatic monitoring system for leaf surface potential of crops at seedling stage according to claim 1, characterized in that: The tray is provided with a groove corresponding to the through hole on the disk, and the groove is used for placing the reference electrode.

8. The automatic monitoring system for leaf surface potential of crops at seedling stage according to claim 7, characterized in that: A telescopic rod is arranged in the groove, and a push plate is arranged at one end of the telescopic rod away from the groove. The push plate is connected to the reference electrode and is used to drive the reference electrode to slide up and down along the side wall of the storage box.

9. The automatic monitoring system for leaf surface potential of crops at seedling stage according to claim 7, characterized in that: A rotating motor is disposed on the lower end surface of the tray, and a positioning member is disposed on the upper end surface of the tray. The positioning member is electrically connected to the rotating motor and is used to monitor the positional relationship between the groove and the through hole and control the start and stop of the rotating motor.

10. A method for automatically monitoring leaf surface potential of crops at seedling stage, characterized in that: The automatic monitoring system for leaf surface potential of crops at seedling stage as claimed in any one of claims 1 to 9 comprises the following steps: Step S10. Prepare a number of crops to be tested and place them on the reference electrode on the tray; Step S20. Start the transport assembly to drive the tray to move to the bottom of the disk, and stop moving when the through hole and the reference electrode overlap in the vertical direction; Step S30: The reference electrode drives the crop to be detected to rise in the vertical direction, pass through the through hole and enter the storage box, and stops when it contacts the recording electrode on the top of the storage box; Step S40. After the top of the crop to be detected contacts the recording electrode, the reference electrode moves downward by a reserved distance and waits for the crop to be detected to grow by itself; Step S50: When the top of the crop to be detected contacts the recording electrode again, the light assembly is turned on and timing is performed, and the shielding member rotates at the same time; Step S60: The signal acquisition probe monitors and sends the data to the host computer for processing and display; Step S70. After the timing of the light assembly ends, the light assembly is turned off, the shielding member stops rotating, and the reference electrode is lowered back onto the tray; Step S80: The transport assembly moves to drive another tray to move directly below the disc, and steps S20 to S80 are repeated.

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