Preparation method and application of recyclable organic photovoltaic antibacterial catalyst with weak light response

Abstract

The invention discloses a preparation method and application of a recyclable organic photovoltaic antibacterial catalyst with weak light response, and the method comprises the following operation steps: A, crushing a carbon material to 3000-5000 meshes to obtain a carbon-based material; b, an organic photovoltaic material and chloroform are mixed according to the following weight ratio: 0.6 mg of the organic photovoltaic material and 10 mL of chloroform, a mixed solution is obtained, the organic photovoltaic material is PTQ10: Y6, and PTQ10: Y6 is prepared by mixing PTQ10 and Y6 according to the weight ratio of 1: 1; and C, uniformly coating the mixed solution obtained in the step B on the carbon-based material obtained in the step A according to a weight ratio of the mixed solution to the carbon-based material of 1: 5000, and drying to obtain the organic photovoltaic antibacterial catalyst which has weak light response and can be recycled. The organic photovoltaic antibacterial catalyst disclosed by the invention has excellent bactericidal ability to acinetobacter baumannii, is simple and convenient to use, can be repeatedly used, is high in safety, can generate a large number of peroxide species in a short time under a weak light condition, induces the acinetobacter baumannii to die by attacking bacterial cell membranes, also has good bactericidal ability in vivo, and can be used for preparing the antibacterial agent for the acinetobacter baumannii. The wound healing can be promoted.

Description

Technical Field

[0001] The invention belongs to the technical field of preparing photocatalytic antibacterial agents, and specifically is a preparation method and application of an organic photovoltaic antibacterial catalyst with weak light response and recyclability. Background Art

[0002] Acinetobacter baumannii is a highly invasive and multidrug-resistant Gram-negative bacterium. It is an important pathogen causing nosocomial infections in today's healthcare system. Infections with Acinetobacter baumannii can cause pneumonia, meningitis, post-traumatic intracranial infection, bacteremia, and cellulitis, among others, and can form biofilms on a variety of surfaces, including non-biological materials such as stainless steel and polypropylene, as well as biological materials such as host epithelial cells, such as the skin, soft tissues, and invasive devices (such as endotracheal tubes and urinary catheters used for mechanical ventilation) of infected persons. Therefore, Acinetobacter baumannii often infects patients in hospitals, especially those in intensive care units, and these infections can be fatal.

[0003] In recent years, Acinetobacter baumannii has shown a tendency to resist a variety of antibiotics, and its resistance mechanisms include but are not limited to the formation of biofilms, multidrug efflux pumps, carbapenemases, changes in permeability and targeting, etc. In current clinical research and practice, combined antibiotic combinations have been widely studied and applied, but Acinetobacter baumannii has also begun to develop certain resistance to combined antibiotic treatment; the discovery and mining of new antibiotics takes a long time, and they are in short supply; there are also many active ingredients in natural products that inhibit Acinetobacter baumannii, but the administration concentration is large, which is not conducive to actual clinical application. Therefore, it is urgent to find new drugs or therapies to fight Acinetobacter baumannii. Photodynamic therapy, as an in vitro therapy, has high safety and has been widely verified in clinical practice, but the use of photosensitizers on the market is relatively complicated, the light response surface is narrow, and the synthesis method is complex. Therefore, it is urgent to develop a safe, simple, efficient and economical organic photovoltaic antibacterial catalyst for the prevention and treatment of Acinetobacter baumannii infection. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide a preparation method and application of an organic photovoltaic antibacterial catalyst with weak light response and recyclability. The organic photovoltaic antibacterial catalyst obtained by the method has excellent bactericidal ability against Acinetobacter baumannii, is easy to use, reusable, and highly safe.

[0005] The present invention solves the above technical problems with the following technical solutions:

[0006] The present invention provides a method for preparing an organic photovoltaic antibacterial catalyst having a weak light response and being recyclable, the method comprising the following steps:

[0007] A. Grind the carbon material into 3000-5000 meshes to obtain carbon-based materials;

[0008] B. Mixing an organic photovoltaic material and chloroform in the following weight ratio: 0.6 mg of organic photovoltaic material and 10 mL of chloroform to obtain a mixed solution, wherein the organic photovoltaic material is PTQ10:Y6, and the PTQ10:Y6 is prepared by mixing PTQ10 and Y6 in a weight ratio of 1:1;

[0009] C. The mixed solution of step B is evenly coated on the carbon-based material of step A, and the weight ratio of the mixed solution to the carbon-based material is 1:5000. After drying, an organic photovoltaic antibacterial catalyst with weak light response and recyclability is obtained.

[0010] In step A of the present invention, when the carbon material is crushed, the carbon material is placed in a wall breaking machine for crushing, and the carbon material crushing time is 30 to 60 seconds.

[0011] In step B of the present invention, when the organic photovoltaic material PTQ10:Y6 is mixed with chloroform, stirring is adopted, and the stirring speed is 500 r / min, the stirring temperature is 25° C., and the stirring time is 8 hours.

[0012] In step C of the present invention, drying refers to drying in a constant temperature drying oven at a temperature of 60° C. for 24 hours.

[0013] Application of the organic photovoltaic antibacterial catalyst with weak light response and recyclability of the present invention:

[0014] Application method 1: the organic photovoltaic antibacterial catalyst of the present invention is used to kill Acinetobacter baumannii in vitro. The operation method is: put the organic photovoltaic antibacterial catalyst into a bacterial solution containing Acinetobacter baumannii (A. baumannii), wherein the concentration of Acinetobacter baumannii in the bacterial solution is 10 8 CFU / mL, the dosage of organic photovoltaic antibacterial catalyst is 0.625-80 mg antibacterial catalyst for every 1 mL of bacterial solution; then LED lamp is used as the light source, and illumination is started after the dark adsorption reaches equilibrium. Samples are taken every 10 minutes, and the concentration of surviving bacteria is determined by the plate counting method, and the killing rate is calculated until the sterilization purpose is achieved.

[0015] The power of the LED lamp is 30W, and the wavelength range is 400-780nm.

[0016] Application method two is to use the organic photovoltaic antibacterial catalyst of the present invention as a drug for treating Acinetobacter baumannii wound infection. When using, the organic photovoltaic antibacterial catalyst is evenly applied to the wound infected with Acinetobacter baumannii, and irradiated with LED light for 30 minutes per day until the wound heals.

[0017] The power of the LED lamp is 30W, and the wavelength range is 400-780nm.

[0018] The present invention has the following beneficial effects:

[0019] (1) The organic photovoltaic antibacterial catalyst obtained by the method of the present invention can remove more than 99% of Acinetobacter baumannii and can be reused.

[0020] (2) The organic photovoltaic antibacterial catalyst of the present invention has photoresponsive properties. When irradiated by weak light, it generates electron-hole pairs, which react with water and oxygen in the environment to generate hydroxyl radicals and superoxide radicals, which penetrate and destroy the cell membrane structure of bacteria, thereby completely killing the bacteria. This process can achieve the purpose of highly efficient inactivation of bacteria without the aid of any foreign chemical substances, and has excellent bactericidal ability, high efficiency and safety.

[0021] (3) The organic photovoltaic antibacterial catalyst of the present invention attacks almost all pathogenic microorganisms including Acinetobacter baumannii by generating reactive oxygen species, while not generating harmful substances. Compared with the prior art, the organic photovoltaic antibacterial catalyst of the present invention can generate a large amount of peroxide species in a short time under weak light conditions, attack the bacterial cell membrane, induce its death, and also has good bactericidal ability in vivo, which can increase the signal molecules of vascular healing in wound tissue, reduce the expression of inflammatory factors, and achieve the purpose of wound healing.

[0022] (4) The organic photovoltaic antibacterial catalyst of the present invention has excellent antibacterial properties and can be used to control outbreaks of Acinetobacter baumannii infections in hospitals, such as in environmental cleaning, medical devices, wound dressings, and other fields. It also has the characteristics of simple preparation process, easy operation, reusability, no drug resistance, safety, high efficiency, and low cost, and has great promotion value.

[0023] (5) The base material used in the preparation method of the organic photovoltaic antibacterial catalyst of the present invention is a natural product carbon-based material, which has many excellent properties such as green, high porosity, strong hydrophilicity, etc., and the material is reusable and degradable, thus achieving a green, pollution-free and environmentally friendly product. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 These are the test results of the effects of different illumination times on Acinetobacter baumannii of the organic photovoltaic antibacterial catalyst of Example 1 of the present invention.

[0025] Figure 2 These are the test results of the effects of different concentrations of organic photovoltaic antibacterial catalysts on Acinetobacter baumannii in Example 2 of the present invention.

[0026] Figure 3 This is the evaluation result of the antibacterial ability of the organic photovoltaic antibacterial catalyst in Example 3 of the present invention during recycling.

[0027] Figure 4 This is the test result of the effect of the organic photovoltaic antibacterial catalyst of Example 3 of the present invention on the drug resistance of Acinetobacter baumannii. In the figure: PEN is the penicillin group, PB is the polymyxin B group, and IPM is the imipenem group.

[0028] Figure 5 These are the in vivo antibacterial ability evaluation results of the organic photovoltaic antibacterial catalyst of Example 4 of the present invention and the control group. Figure 5 (a) is the colonization amount of Acinetobacter baumannii in the wound tissue on the fourth day, Figure 5 (b) is the colonization amount of Acinetobacter baumannii in the wound tissue on the eighth day.

[0029] Figure 6 These are the evaluation results of the wound healing promoting ability of the organic photovoltaic antibacterial catalyst of Example 5 of the present invention and the control group. Figure 6 (a) is a real shot of wound healing. Figure 6 (b) is a simulation diagram of wound healing. DETAILED DESCRIPTION

[0030] The technical solution of the present invention is further described below in conjunction with specific embodiments, but the embodiments do not limit the present invention in any form.

[0031] The photosensitizer concentration described in the following examples refers to the concentration of a mixed solution of organic photovoltaic material and chloroform in a mixed solution of bacterial solution and organic photovoltaic antibacterial catalyst.

[0032] Example 1

[0033] The method for preparing the organic photovoltaic antibacterial catalyst having weak light response and being recyclable of the present invention comprises the following steps:

[0034] A. Put 10g of carbon material into a wall breaking machine and crush it for 50s to obtain 3000 mesh carbon-based material.

[0035] B. Mix the organic photovoltaic material and chloroform in the following weight ratio: organic photovoltaic material PTQ10: Y6 0.6 mg; chloroform 10 mL to obtain a mixed solution. The organic photovoltaic material is prepared by mixing PTQ10 and Y6 in a weight ratio of 1:1. When the organic photovoltaic material and chloroform are mixed, a magnetic stirrer is used to stir the mixture, the stirring speed is 500 r / min, the stirring temperature is 25° C., and the stirring time is 8 hours.

[0036] C. The mixed solution of step B is evenly coated on the carbon-based material of step A, the weight ratio of the mixed solution to the carbon-based material is 1:5000, and then placed in a constant temperature drying oven at 60° C. for 24 hours to obtain an organic photovoltaic antibacterial catalyst with weak light response and recyclability.

[0037] Application method: put the organic photovoltaic antibacterial catalyst obtained in step C into a solution (i.e., bacterial solution) containing Acinetobacter baumannii (A. baumannii) and place it in a reaction container, and use a 30W LED lamp as a light source; when the dark state adsorption reaches equilibrium, start irradiation for 0 to 60 minutes, take a sample every 10 minutes, determine the concentration of surviving bacteria by plate counting method, and calculate the killing rate. The wavelength range of the LED lamp is 400nm, and the concentration of Acinetobacter baumannii in the bacterial solution is 10 8 CFU / mL, the dosage of the organic photovoltaic antibacterial catalyst is 20 mg of antibacterial catalyst per 1 mL of bacterial solution (equivalent to a photosensitizer concentration of 4 μg / mL).

[0038] The antibacterial effect of the above organic photovoltaic antibacterial catalyst is shown in Figure 1 As shown in Figure 1 It can be seen that after 30 minutes of illumination, the killing rate of the organic photovoltaic antibacterial catalyst against Acinetobacter baumannii reached 99%, showing a good antibacterial effect.

[0039] Example 2

[0040] The method for preparing the organic photovoltaic antibacterial catalyst having weak light response and being recyclable of the present invention comprises the following steps:

[0041] A. Put 10g of carbon material into a wall breaker and crush it for 40s to obtain 4000 mesh carbon-based material.

[0042] B. Mix the organic photovoltaic material and chloroform in the following weight ratio: organic photovoltaic material PTQ10: Y6 0.6 mg, chloroform 10 mL, to obtain a mixed solution. The organic photovoltaic material is prepared by mixing PTQ10 and Y6 in a weight ratio of 1:1. When the organic photovoltaic material and chloroform are mixed, a magnetic stirrer is used to stir and mix, the stirring speed is 500 r / min, the stirring temperature is 25° C., and the stirring time is 8 hours.

[0043] C. The mixed solution of step B is evenly coated on the carbon-based material of step A, the weight ratio of the mixed solution to the carbon-based material is 1:5000, and then dried in a constant temperature drying oven at 60° C. for 24 hours to obtain an organic photovoltaic antibacterial catalyst with weak light response and recyclability.

[0044] Application method: put the organic photovoltaic antibacterial catalyst obtained in step C into a solution (i.e. bacterial solution) containing Acinetobacter baumannii (A. baumannii) and place it in a reaction container, and use a 30W LED lamp as a light source; when the dark state adsorption reaches equilibrium, start irradiation, the concentration of the organic photovoltaic antibacterial agent is 0.625-80mg / mL (equivalent to a photosensitizer concentration of 0.125-16μg / mL), irradiate for 30min, take a sample once for each concentration gradient, determine the concentration of surviving bacteria by plate counting method, and calculate the killing rate. The wavelength of the LED lamp is 450nm; the concentration of Acinetobacter baumannii in the bacterial solution is 10 8 CFU / mL.

[0045] The antibacterial effect of the above organic photovoltaic antibacterial catalyst is shown in Figure 2 As shown, from Figure 2 It can be seen that when the concentration of the organic photovoltaic antibacterial agent is 40 mg / mL (equivalent to the concentration of the photosensitizer is 8 μg / mL), the killing rate of Acinetobacter baumannii reaches 100%, which has a good antibacterial effect.

[0046] Example 3

[0047] The method for preparing the organic photovoltaic antibacterial catalyst having weak light response and being recyclable of the present invention comprises the following steps:

[0048] A. Put 10g of carbon material into a wall breaking machine and crush it for 30s to obtain 5000 mesh carbon-based material.

[0049] B. Mix the organic photovoltaic material and chloroform in the following weight ratio: organic photovoltaic material PTQ10: Y6 0.6 mg, chloroform 10 mL, to obtain a mixed solution. The organic photovoltaic material is prepared by mixing PTQ10 and Y6 in a weight ratio of 1:1. When the organic photovoltaic material and chloroform are mixed, a magnetic stirrer is used to stir and mix, the stirring speed is 500 r / min, the stirring temperature is 25° C., and the stirring time is 8 hours.

[0050] C. The mixed solution of step B is evenly coated on the carbon-based material of step A, the weight ratio of the mixed solution to the carbon-based material is 1:5000, and then dried in a constant temperature drying oven at 60° C. for 24 hours to obtain an organic photovoltaic antibacterial catalyst with weak light response and recyclability.

[0051] Application method: The organic photovoltaic antibacterial catalyst obtained in step C is placed in a solution (i.e., bacterial solution) containing Acinetobacter baumannii (A. baumannii), the concentration of the organic photovoltaic antibacterial agent is 20 mg / mL (equivalent to a photosensitizer concentration of 4 μg / mL), placed in a reaction container, and a 30W LED lamp is used as a light source; when the dark state adsorption reaches equilibrium, illumination is started for 30 minutes, and the bacterial solution after the action is taken for continued cultivation, and the organic photovoltaic antibacterial agent material is rinsed with physiological saline and placed in a 60°C oven for drying for 24 hours, and then continuously recycled for 10 times, and a sample is taken each time, and the concentration of surviving bacteria is determined by the plate count method to calculate the killing rate. The Acinetobacter baumannii bacterial solution stimulated each time is evenly spread on the plate, and its resistance to antibiotics is identified using imipenem, penicillin and polymyxin B drug sensitivity tablets. The wavelength range of the LED lamp is 780nm; the concentration of Acinetobacter baumannii in the bacterial solution is 10 8 CFU / mL.

[0052] The antibacterial effect of this example is shown in Figure 3 As shown, from Figure 3 It can be seen that after the organic photovoltaic antibacterial catalyst was used for 10 consecutive times, its bactericidal effect remained consistent without significant changes, indicating that the organic photovoltaic antibacterial catalyst can be used continuously and the antibacterial effect has not weakened. Figure 4 It can be seen that the Acinetobacter baumannii, which was stimulated by the organic photovoltaic antibacterial catalyst for 10 consecutive times, did not develop resistance to the three antibiotics penicillin (PEN), imipenem (IPM) and polymyxin B (PB), and the average diameters of their inhibition zones were 6mm, 15.11mm and 24.48mm, respectively.

[0053] Example 4

[0054] The method for preparing the organic photovoltaic antibacterial catalyst having weak light response and being recyclable of the present invention comprises the following steps:

[0055] A. Put 10g of carbon material into a wall breaking machine and crush it for 50s to obtain 5000 mesh carbon-based material.

[0056] B. Mix the organic photovoltaic material and chloroform in the following weight ratio: organic photovoltaic material PTQ10: Y6 0.6 mg, chloroform 10 mL, to obtain a mixed solution. The organic photovoltaic material is prepared by mixing PTQ10 and Y6 in a weight ratio of 1:1. When the organic photovoltaic material and chloroform are mixed, a magnetic stirrer is used to stir and mix, the stirring speed is 500 r / min, the stirring temperature is 25° C., and the stirring time is 8 hours.

[0057] C. The mixed solution of step B is evenly coated on the carbon-based material of step A, the weight ratio of the mixed solution to the carbon-based material is 1:5000, and then dried in a constant temperature drying oven at 60° C. for 24 hours to obtain an organic photovoltaic antibacterial catalyst with weak light response and recyclability.

[0058] Application method: remove the hair on the back of the mouse, inject Avertin subcutaneously to anesthetize the mouse, cut a circular wound with a diameter of 10min on its back, wipe a little blood with a sterile cotton ball, and drip the Acinetobacter baumannii suspension into the open wound. After 24 hours, the Acinetobacter baumannii wound infection model can be established. The concentration of the Acinetobacter baumannii suspension is 10 9 CFU / mL, the dosage is 30μL.

[0059] The wounds were treated with physiological saline (Model group), organic photovoltaic antibacterial catalyst (high-dose group HDG, 80 mg, equivalent to 16 μg of photosensitizer; low-dose group LDG, 20 mg, equivalent to 4 μg of photosensitizer), MEM (5 mM, meropenem positive control) and H2O2 solution (5 mM, hydrogen peroxide positive control). The organic photovoltaic antibacterial catalyst obtained in step C of this embodiment was evenly applied to the wound of the Acinetobacter baumannii wound infection model obtained after the above treatment, and irradiated with a 30W LED lamp for 30 minutes, and the wavelength of the LED lamp was 600 nm. After that, the antibacterial catalyst was gently washed off with physiological saline. Once a day, the drug was administered for 9 consecutive days. The mice were killed on the 4th and 8th days, and the wound tissue was taken. After homogenization, the colonization of Acinetobacter baumannii was checked.

[0060] The antibacterial effect of the organic photovoltaic antibacterial catalyst obtained in this example is shown in Figure 5 As shown, from Figure 5 It can be seen that after 30 minutes of illumination, the high-dose organic photovoltaic antibacterial catalyst of the present invention (80 mg, equivalent to 16 μg of photosensitizer) has the best in vivo killing effect on Acinetobacter baumannii, and the bactericidal effect on the 4th and 8th days is significantly better than that of the Model group, MEM group and H2O2 group.

[0061] Example 5

[0062] The method for preparing the organic photovoltaic antibacterial catalyst having weak light response and being recyclable of the present invention comprises the following steps:

[0063] A. Put 10g of carbon material into a wall breaking machine and crush it for 50s to obtain 5000 mesh carbon-based material.

[0064] B. Mix the organic photovoltaic material and chloroform in the following weight ratio: organic photovoltaic material PTQ10: Y6 0.6 mg, chloroform 10 mL, to obtain a mixed solution. The organic photovoltaic material is prepared by mixing PTQ10 and Y6 in a weight ratio of 1:1. When the organic photovoltaic material and chloroform are mixed, a magnetic stirrer is used to stir and mix, the stirring speed is 500 r / min, the stirring temperature is 25° C., and the stirring time is 8 hours.

[0065] C. The mixed solution of step B is evenly coated on the carbon-based material of step A, the weight ratio of the mixed solution to the carbon-based material is 1:5000, and then dried in a constant temperature drying oven at 60° C. for 24 hours to obtain an organic photovoltaic antibacterial catalyst with weak light response and recyclability.

[0066] Application method: First, remove the hair on the back of the mouse, inject Avertin subcutaneously to anesthetize the mouse, cut a circular wound with a diameter of 10min on its back, wipe a little blood with a sterile cotton ball, and drip the Acinetobacter baumannii suspension into the open wound. After 24 hours, the Acinetobacter baumannii wound infection model can be established. The concentration of the Acinetobacter baumannii suspension is 10 9 CFU / mL, the dosage is 30μL.

[0067] The wounds were treated with physiological saline (Model group), organic photovoltaic antibacterial catalyst (high-dose group HDG, 80 mg, equivalent to 16 μg of photosensitizer; low-dose group LDG, 20 mg, equivalent to 4 μg of photosensitizer), MEM (5 mM, meropenem positive control) and H2O2 solution (5 mM, hydrogen peroxide positive control). The organic photovoltaic antibacterial catalyst obtained in step C of this embodiment was evenly applied to the wound of the Acinetobacter baumannii wound infection model obtained by the above treatment, and irradiated with a 30W LED lamp for 30 minutes, the wavelength of the LED lamp was 780 nm. The degree of wound healing was continuously observed and recorded.

[0068] The antibacterial effect of the organic photovoltaic antibacterial catalyst obtained in this example is shown in Figure 6 As shown, from Figure 6 It can be seen that during the 9 days of light treatment, the organic photovoltaic antibacterial catalyst can significantly help the wounds of mice to heal quickly. The wounds of mice can be restored to normal levels on the 12th day after using the organic photovoltaic antibacterial catalyst of the present invention.

Claims

1. A method for preparing a recyclable organic photovoltaic antibacterial catalyst with weak light response, characterized in that: The method comprises the following steps: A. Grind the carbon material into 3000-5000 meshes to obtain carbon-based materials; B. Mixing an organic photovoltaic material and chloroform in the following weight ratio: 0.6 mg of organic photovoltaic material and 10 mL of chloroform to obtain a mixed solution, wherein the organic photovoltaic material is PTQ10:Y6, and the PTQ10:Y6 is prepared by mixing PTQ10 and Y6 in a weight ratio of 1:1; C. The mixed solution of step B is evenly coated on the carbon-based material of step A, and the weight ratio of the mixed solution to the carbon-based material is 1:5000. After drying, an organic photovoltaic antibacterial catalyst with weak light response and recyclability is obtained.

2. The method for preparing the recyclable organic photovoltaic antibacterial catalyst with weak light response according to claim 1, characterized in that: In the step A, the carbon material is crushed by placing the carbon material in a wall breaking machine, and the carbon material crushing time is 30 to 60 seconds.

3. The method for preparing the recyclable organic photovoltaic antibacterial catalyst with weak light response according to claim 1 or 2, characterized in that: In the step B, the organic photovoltaic material PTQ10:Y6 is mixed with chloroform by stirring, with a stirring speed of 500 r / min, a stirring temperature of 25° C., and a stirring time of 8 h.

4. The method for preparing the recyclable organic photovoltaic antibacterial catalyst with weak light response according to claim 1 or 2, characterized in that: In the step C, drying refers to drying in a constant temperature drying oven at 60° C. for 24 hours.

5. The use of an organic photovoltaic antibacterial catalyst having weak light response and recyclable according to any one of the preparation methods of claims 1 to 4, characterized in that: The organic photovoltaic antibacterial catalyst is used to kill Acinetobacter baumannii in vitro. The operation method is as follows: the organic photovoltaic antibacterial catalyst is placed in a bacterial solution containing Acinetobacter baumannii (A. baumannii), and the concentration of Acinetobacter baumannii in the bacterial solution is 10 8 CFU / mL, the dosage of organic photovoltaic antibacterial catalyst is 0.625-80 mg antibacterial catalyst for every 1 mL of bacterial solution; then LED lamp is used as the light source, and illumination is started after the dark adsorption reaches equilibrium. Samples are taken every 10 minutes, and the concentration of surviving bacteria is determined by the plate counting method, and the killing rate is calculated until the sterilization purpose is achieved.

6. The use of the organic photovoltaic antibacterial catalyst with weak light response and recyclability according to claim 5, characterized in that: The power of the LED lamp is 30W, and the wavelength range is 400-780nm.

7. Use of an organic photovoltaic antibacterial catalyst having weak light response and recyclable according to any one of the preparation methods of claims 1 to 4, characterized in that: The organic photovoltaic antibacterial catalyst is used as a drug for treating Acinetobacter baumannii wound infection. During application, the organic photovoltaic antibacterial catalyst is evenly applied to the wound infected with Acinetobacter baumannii, and irradiated with LED light for 30 minutes a day until the wound heals.

8. The use of the organic photovoltaic antibacterial catalyst with weak light response and recyclability according to claim 7, characterized in that: The power of the LED lamp is 30W, and the wavelength range is 400-780nm.

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