Method and device for collecting terrestrial animal tissue sample

Abstract

The invention discloses a terrestrial animal tissue sample collection method and device. The method comprises the following steps: S1, preparing a non-Newtonian fluid by using a stirring device; s2, guiding the animal to a standing platform; s3, the four containers are lifted through a lifting device and arranged below the four limbs of the animal in a sleeving mode; s4, inputting the non-Newtonian fluid into the container to fix the four limbs of the animal; and S5, carrying out tissue sampling on the animal. When animal tissues are sampled, the discomfort and stress response of animals are reduced, and the physiological health of the animals is guaranteed to the greatest extent, so that the accuracy and reliability of sampling results are improved. The animal can be stably and safely fixed in the binding process, and the risk that experimenters are injured by counterattack or struggling of the animal in the sampling process is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of animal tissue sample collection, and in particular to a terrestrial animal tissue sample collection method and device. Background Art

[0002] In the fields of biomedicine, animal experiments, veterinary diagnosis, etc., animal tissue sample collection is a common operation. Traditional animal tissue sample collection methods usually involve fixing or restraining the animal to prevent it from struggling during the operation, thereby ensuring the smooth progress of sampling. However, existing animal restraint technologies and methods generally have some limitations, especially in terms of the strength of animal restraint, control of restraint time, and animal comfort. Most of the existing animal restraint devices use physical mechanical fixation methods, such as belts, straps, restraint nets, etc. These methods usually exert a certain amount of oppression on the animal, causing discomfort or stress reactions in the animal.

[0003] The stress response of animals may cause abnormalities in physiological parameters (such as blood pressure, body temperature, hormone levels, etc.) in the body, affecting the accuracy of the sampling results. For example, the levels of certain hormones in the blood (such as cortisol) may be high due to stress, interfering with the interpretation of experimental data. Stress will also affect the physiological state of the animal. The collected tissue samples may not be representative or have physiological abnormalities due to the influence of stress, affecting the reliability of the experimental results. For example, the cell components, immune responses, metabolites, etc. in the blood may be different from those in the normal state, leading to experimental errors. Not only will it damage the physiological health of the animals, it may also lead to inaccurate and unreliable experimental results. Summary of the invention

[0004] The purpose of this section is to summarize some aspects of embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and the invention title of this application to avoid blurring the purpose of this section, the specification abstract and the invention title, and such simplifications or omissions cannot be used to limit the scope of the present invention.

[0005] In view of the low efficiency of the existing peppermint oil extraction, a new method and device for collecting terrestrial animal tissue samples is proposed.

[0006] To solve the above technical problems, the present invention provides the following technical solutions: a method for collecting tissue samples of terrestrial animals, S1: using a stirring device to prepare a non-Newtonian fluid; S2: guiding the animal to a standing platform; S3: raising four containers by a lifting device and putting them under the animal's limbs; S4: inputting the non-Newtonian fluid into the container to fix the animal's limbs; S5: taking tissue samples from the animal.

[0007] As a preferred embodiment of the method for collecting terrestrial animal tissue samples of the present invention, the preparation method of S1 includes preparing a low binding solution, a medium binding solution, and a high binding solution according to different mass ratios of water and corn starch, for binding different animals.

[0008] As a preferred embodiment of the method for collecting tissue samples of terrestrial animals described in the present invention, the size and shape of the fixed container can be adjusted according to the size of the animal's limbs.

[0009] In order to solve the above technical problems, the present invention provides the following technical solutions: a terrestrial animal tissue sample collection device, comprising a standing platform, a support platform arranged on the standing platform, a fixed container slidably sleeved on the outside of the support platform, a lifting component arranged below the fixed container, a stirring component arranged near the fixed container, and a non-Newtonian fluid arranged in the stirring component; the non-Newtonian fluid is prepared by the stirring component, and then the non-Newtonian fluid is injected into the fixed container in which the animal stands, so as to fix the animal's limbs.

[0010] As a preferred solution of the terrestrial animal tissue sample collection device of the present invention, the fixed container is a bottomless shell, and the internal shape is the same as the shape of the support platform.

[0011] As a preferred embodiment of the terrestrial animal tissue sample collection device described in the present invention, the lifting assembly includes a mounting platform arranged below the support platform, a mounting block arranged on the mounting platform, a toothed disc arranged outside the mounting platform, a gear meshing with the toothed disc, a rotating rod connecting the gear and the mounting block, a first connecting rod connected to one end of the rotating rod, a second connecting rod connected to the other end of the first connecting rod, and a lifting platform connected to one end of the second connecting rod; the rotating rod passes through the mounting block, one end of the rotating rod is connected to the gear, and the other end is connected to the first connecting rod.

[0012] As a preferred embodiment of the terrestrial animal tissue sample collection device of the present invention, the stirring assembly includes a support frame arranged near the fixed container, a shell arranged on the support frame, a feeding port opened on the shell, a water injection port opened on the shell, a discharge port opened at the bottom of the shell, an agitator arranged in the shell, and a heating plate arranged in the shell.

[0013] As a preferred solution of the terrestrial animal tissue sample collection device of the present invention, the support platform and the fixed container are detachable parts, and the installation height of the support platform is the same as that of the standing platform.

[0014] The beneficial effects of the terrestrial animal tissue sample collection method and device of the present invention are as follows: the animal's limbs are restrained by non-Newtonian fluids. This restraint method is gentler than traditional mechanical fixing methods such as belts, straps, and restraint nets. It can effectively restrain the animal's limbs without causing excessive pressure on the animal, reduce the animal's discomfort and stress response, and maximize the protection of the animal's physiological health. It reduces the abnormal fluctuations of physiological parameters such as blood pressure, body temperature, and hormone levels caused by stress, thereby improving the accuracy and reliability of the sampling results. It can also avoid the risk of animal injury caused by too tight or too loose restraint in traditional restraint methods, ensure that the animal is stably and safely fixed during the restraint process, and reduce the risk of experimental personnel being injured by the animal's counterattack or struggle during the sampling process. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative labor. Among them:

[0016] Figure 1 It is a schematic diagram of the overall structure of the terrestrial animal tissue sample collection device of the present invention.

[0017] Figure 2 It is a schematic diagram of the structure of the lifting component of the terrestrial animal tissue sample collection device of the present invention.

[0018] Figure 3 It is a schematic diagram of the structure of the stirring component of the terrestrial animal tissue sample collection device of the present invention.

[0019] Figure 4 It is a cross-sectional view of the stirring component structure of the terrestrial animal tissue sample collection device of the present invention. DETAILED DESCRIPTION

[0020] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the accompanying drawings.

[0021] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0022] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0023] Secondly, the present invention is described in detail with reference to the schematic diagram. When describing the embodiments of the present invention in detail, for the sake of convenience, the cross-sectional diagrams showing the device structure will not be partially enlarged according to the general scale, and the schematic diagrams are only examples, which should not limit the scope of protection of the present invention. In addition, in actual production, the three-dimensional dimensions of length, width and depth should be included.

[0024] Example 1

[0025] Provided is a method for collecting tissue samples from terrestrial animals, comprising: S1: preparing a non-Newtonian fluid using a stirring device; S2: guiding the animal to a standing platform; S3: raising four containers by a lifting device and placing them under the animal's limbs; S4: injecting the non-Newtonian fluid into the containers to fix the animal's limbs; and S5: taking tissue samples from the animal.

[0026] Furthermore, the preparation method of S1 includes preparing a low-binding solution, a medium-binding solution, and a high-binding solution according to different mixing mass ratios of water and corn starch, for binding different animals.

[0027] The size and shape of the fixed container can be adjusted according to the size of the animal's limbs.

[0028] The unique rheological properties of non-Newtonian fluids cause their viscosity to increase rapidly when subjected to external forces, showing solid characteristics; when the external force is removed, the fluid quickly recovers its fluidity. It is this property that enables non-Newtonian fluids to flexibly control the strength of restraint during the restraint process, maintaining sufficient fixing strength when external forces are applied, and quickly loosening after the operation, reducing the continuous pressure and stress response on the animal.

[0029] According to the requirements of different animal species, body shapes and restraint strength, the preparation of non-Newtonian fluids can be divided into the following three different ratios, suitable for different application scenarios:

[0030] Low binding solution: water: corn starch = 1:1 (mass ratio)

[0031] Suitable for lower strength restraint needs, with strong fluidity. This solution can provide a gentler restraint effect, suitable for small and flexible animals, or operations that only need to be slightly fixed for observation and measurement. Its low viscosity allows the animal to be restrained comfortably while still ensuring sufficient stability.

[0032] Medium binding solution: water: corn starch = 1.5:1 (mass ratio)

[0033] Suitable for applications that balance restraint strength and fluidity. This ratio of solution provides a certain compromise between fluidity and restraint, and can meet the restraint requirements of most common animals (such as medium-sized mammals, poultry, etc.). Its moderate viscosity enables it to provide sufficient fixing force while reducing discomfort during the restraint process, making it suitable for long-term operations.

[0034] High binding solution: water: corn starch = 2:1 (mass ratio)

[0035] Suitable for applications that require strong binding force and fast hardening. The solution has a high viscosity and can solidify quickly in a short time. It is suitable for larger and stronger animals, or situations where fixation and sampling need to be completed in a short time. The high viscosity fluid can effectively resist the external force generated by the animal's struggle, ensuring the smooth progress of the sampling process.

[0036] Example 2

[0037] Reference Figures 1 to 4 , this embodiment is different from the first embodiment in that: a terrestrial animal tissue sample collection device is provided, comprising a standing platform 100, a support platform 101 arranged on the standing platform 100, a fixed container 102 slidably sleeved on the outside of the support platform 101, a lifting component 103 arranged below the fixed container 102, a stirring component 104 arranged near the fixed container 102, and a non-Newtonian fluid arranged in the stirring component 104; the non-Newtonian fluid is prepared by the stirring component 104, and then the non-Newtonian fluid is injected into the fixed container 102 where the animal stands, so as to fix the animal's limbs. Among them, the animal to be sampled can reach the support platform 101 fixedly arranged on the ground from the standing platform 100, and there are four support platforms 101 corresponding to the animal's limbs. There are also four fixed containers 102, which can be raised or lowered by the lifting component 103. When rising, they can form four containers with the support platform 101, which can respectively cover the animal's limbs.

[0038] Specifically, the fixed container 102 is a bottomless shell, and its internal shape is the same as that of the support platform 101. The inner surface of the fixed container 102 conflicts with the side of the support platform 101, and the inner surface of the fixed container 102 can slide relative to the side of the support platform 101.

[0039] Furthermore, the lifting assembly 103 includes a mounting platform 103a arranged below the support platform 101, a mounting block 103b arranged on the mounting platform 103a, a toothed disc 103c arranged on the outside of the mounting platform 103a, a gear 103d meshing with the toothed disc 103c, a rotating rod 103e connecting the gear 103d and the mounting block 103b, a first connecting rod 103f connected to one end of the rotating rod 103e, a second connecting rod 103g connected to the other end of the first connecting rod 103f, and a lifting platform 103h connected to one end of the second connecting rod 103g; the rotating rod 103e passes through the mounting block 103b, one end of the rotating rod 103e is connected to the gear 103d, and the other end is connected to the first connecting rod 103f. Among them, the toothed disc 103c is rotatably sleeved on the outer side of the mounting block 103b, four gears 103d are taken, and are equidistantly arranged on the toothed disc 103c. The rotation of the toothed disc 103c can simultaneously drive the four gears 103d to rotate, and the four fixed containers 102 are fixedly mounted on the lifting platform 103h.

[0040] Further, the stirring assembly 104 includes a support frame 104a arranged near the fixed container 102, a shell 104b arranged on the support frame 104a, a feed port 104c opened on the shell 104b, a water injection port 104d opened on the shell 104b, a discharge port 104e opened at the bottom of the shell 104b, an agitator 104f arranged in the shell 104b, and a heating plate 104g arranged in the shell 104b. Among them, the shell 104b is a transparent material, and the water in the shell 104b is heated by the heating plate 104g, which can help the corn starch to dissolve better. When the temperature of the non-Newtonian fluid is too low, the corn starch aqueous solution may become too viscous and may even solidify, so it is also necessary to ensure that the temperature of the fluid in the pipeline is within an appropriate range through the heating plate 104g. Corn starch aqueous solution may be stratified during long-term standing or transportation. Especially when the fluid concentration is high, starch particles are easy to precipitate. The stirring component 104 can maintain the uniformity of the fluid.

[0041] The support platform 101 and the fixed container 102 are detachable parts, and the installation height of the support platform 101 is the same as that of the standing platform 100. The support platform 101 and the fixed container 102 can be replaced according to the size of different animals to avoid wasting non-Newtonian fluid.

[0042] The rest of the structure is the same as that of Example 1.

[0043] Operation process: prepare non-Newtonian fluid and select different support platforms 101 and fixed containers 102 according to the size of the animal to be sampled, use a stirring device to prepare non-Newtonian fluid, guide the animal to the standing platform 100, start the lifting device, one of the gears 103d in the lifting device rotates, driving the toothed disc 103c to rotate, the rotation of the toothed disc 103c drives the other three gears 103d to rotate, these four gears 103d rotate at the same time, driving the four first connecting rods 103f and the second connecting rods 103g to rotate at the same time, driving the lifting platform 103h to rise smoothly, and the four fixed containers 102 are lifted by the lifting platform 103h and put under the animal's limbs; the non-Newtonian fluid is input into the container manually or through a pipeline, the animal's limbs are fixed, and tissue sampling is performed on the animal.

[0044] Beneficial effects: When sampling animal tissues, it reduces the discomfort and stress response of animals, maximizes the physiological health of animals, and thus improves the accuracy and reliability of sampling results. It also ensures that animals are stably and safely fixed during the restraint process, reducing the risk of experimenters being injured by animals fighting back or struggling during the sampling process.

[0045] Importantly, it should be noted that the construction and arrangement of the present application shown in a plurality of different exemplary embodiments are only exemplary. Although only a few embodiments are described in detail in this disclosure, it should be readily understood by those who refer to this disclosure that many modifications are possible (e.g., the size, scale, structure, shape and proportion of various elements, and parameter values ​​(e.g., temperature, pressure, etc.), installation arrangement, use of materials, color, directional changes, etc.) without substantially departing from the novel teachings and advantages of the subject matter described in the application. For example, the element shown as integrally formed can be composed of multiple parts or elements, the position of the element can be inverted or otherwise changed, and the nature or number or position of the discrete element can be changed or changed. Therefore, all such modifications are intended to be included in the scope of the present invention. The order or sequence of any process or method steps can be changed or reordered according to alternative embodiments. In the claims, any "device plus function" clause is intended to cover the structure of performing the function described herein, and is not only structurally equivalent but also equivalent structure. Without departing from the scope of the present invention, other replacements, modifications, changes and omissions can be made in the design, operating conditions and arrangement of the exemplary embodiments. Therefore, the invention is not limited to a specific embodiment, but extends to several modifications still falling within the scope of the appended claims.

[0046] Additionally, in order to provide a concise description of exemplary embodiments, all features of an actual embodiment (ie, those features that are not relevant to the best mode presently contemplated for carrying out the invention or those that are not relevant to implementing the invention) may not be described.

[0047] It will be appreciated that in the development of any actual implementation, as in any engineering or design project, numerous implementation-specific decisions may be made. Such a development effort may be complex and time-consuming, but will be a routine task of design, fabrication, and production for those of ordinary skill having the benefit of this disclosure without undue experimentation.

[0048] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A method for collecting tissue samples of terrestrial animals, characterized in that: include, S1: Preparation of non-Newtonian fluid using a stirring device; S2: guide the animal to the standing platform; S3: four containers are raised by a lifting device and placed under the animal's limbs; S4: non-Newtonian fluid is introduced into the container to fix the animal's limbs; S5: Perform tissue sampling on animals.

2. The method for collecting terrestrial animal tissue samples according to claim 1, characterized in that: The preparation method of S1 comprises preparing a low-binding solution, a medium-binding solution and a high-binding solution according to different mixing mass ratios of water and corn starch, and the solutions are used to bind different animals.

3. The method for collecting terrestrial animal tissue samples according to claim 1, characterized in that: The size and shape of the holding container can be adjusted to the size of the animal's limbs.

4. A terrestrial animal tissue sample collection device, characterized in that: The terrestrial animal tissue sample collection method according to any one of claims 1 to 3, the terrestrial animal tissue sample collection device comprising a standing platform (100), a support platform (101) arranged on the standing platform (100), a fixed container (102) slidably sleeved on the outside of the support platform (101), a lifting component (103) arranged below the fixed container (102), a stirring component (104) arranged near the fixed container (102), and a non-Newtonian fluid arranged in the stirring component (104); A non-Newtonian fluid is prepared by a stirring component (104), and then the non-Newtonian fluid is injected into a fixed container (102) in which an animal stands, so as to fix the animal's limbs.

5. The terrestrial animal tissue sample collection device according to claim 4, characterized in that: The fixed container (102) is a bottomless shell, and the internal shape is the same as that of the support platform (101).

6. The terrestrial animal tissue sample collection device according to claim 4, characterized in that: The lifting assembly (103) comprises a mounting platform (103a) arranged below the support platform (101), a mounting block (103b) arranged on the mounting platform (103a), a toothed disc (103c) arranged outside the mounting platform (103a), a gear (103d) meshing with the toothed disc (103c), a rotating rod (103e) connecting the gear (103d) and the mounting block (103b), a first connecting rod (103f) connected to one end of the rotating rod (103e), a second connecting rod (103g) connected to the other end of the first connecting rod (103f), and a lifting platform (103h) connected to one end of the second connecting rod (103g); The rotating rod (103e) passes through the mounting block (103b), one end of the rotating rod (103e) is connected to the gear (103d), and the other end is connected to the first connecting rod (103f).

7. The terrestrial animal tissue sample collection device according to claim 4, characterized in that: The stirring assembly (104) includes a support frame (104a) arranged near the fixed container (102), a shell (104b) arranged on the support frame (104a), a feeding port (104c) opened on the shell (104b), a water injection port (104d) opened on the shell (104b), a discharge port (104e) opened at the bottom of the shell (104b), an agitator (104f) arranged in the shell (104b), and a heating plate (104g) arranged in the shell (104b).

8. The terrestrial animal tissue sample collection device according to claim 4, characterized in that: The support platform (101) and the fixed container (102) are detachable parts, and the installation height of the support platform (101) is the same as that of the standing platform (100).

Images