Device for quantitatively feeding hirudin eluent

Abstract

The device comprises a feeding assembly, a liquid storage box and a chromatography assembly, the liquid storage box and the chromatography assembly are connected with the feeding assembly, the feeding assembly is provided with an outer cylinder, a rotating cylinder and a sealing cavity from outside to inside, and the rotating cylinder is driven by a driving assembly to rotate and then communicates with the liquid storage box and the feeding assembly; or the chromatography assembly and the feeding assembly are communicated, and finally the elution agent is added into the chromatography assembly through the piston. The elution agent can be directly injected into the chromatography assembly in a closed environment, exposure in a laboratory is avoided, quantitative adding can be achieved, and the volume of the elution agent in the chromatography assembly is strictly controlled.

Description

Technical Field

[0001] The present application belongs to the technical field of hirudin purification, and specifically relates to a device for quantitatively administering a hirudin de-eluent. Background Art

[0002] Hirudin is a biologically active substance extracted from leeches, which is mainly used for anticoagulation and promoting blood circulation. Purified hirudin can be used in medical research, pharmaceutical development and other fields.

[0003] When purifying hirudin in practice, a quantitative de-eluent needs to be added to the ion exchange column. In order to avoid the de-eluent being contaminated by the experimental environment, manual addition is generally not adopted. Usually, laboratory personnel need to put the de-eluent in a closed container and then add it quantitatively. The prior art lacks a device for realizing simple quantitative addition of de-eluent in a closed space, and too much or too little de-eluent will affect the actual purification effect. Summary of the invention

[0004] The present application provides a device for quantitatively administering a hirudin desolvant, which can achieve quantitative administration of the desolvant in a closed environment to avoid contamination.

[0005] The technical solution of this application is as follows: A device for quantitatively administering a hirudin de-eluent comprises a feeding component and a liquid storage box and a chromatography component respectively connected to the feeding component; The feeding assembly comprises an outer cylinder, a sealing cavity and a rotating cylinder arranged between the outer cylinder and the sealing cavity; The outer cylinder is provided with a liquid inlet pipe and a liquid outlet pipe respectively communicating with the liquid storage box and the chromatography component; the sealed cavity is provided with a first circular hole and a second circular hole respectively aligned with the liquid inlet pipe and the liquid outlet pipe; A chamber is provided between the outer cylinder and the rotating cylinder, and the chamber is provided with a driving assembly for driving the rotating cylinder to rotate, the driving assembly is driven by a threaded rotating handle and is connected to the rotating cylinder through a limit ring, a square block, and a protrusion in sequence; the protrusion protrudes toward the rotating cylinder and the rotating cylinder has a guide groove matched with the protrusion, the guide groove has a starting end and a tail end and spirally extends from the starting end to the tail end; The drum has an inflow hole and an outflow hole, and the inflow hole and the outflow hole are configured such that when the protrusion is located at the starting end, the inflow hole is aligned with the first circular hole, and when the protrusion is located at the trailing end, the outflow hole is aligned with the second circular hole; The sealing cavity is provided with a piston extending from the inside to the outside, and the piston includes a piston head located inside the sealing cavity and a piston rod located outside. The end of the piston rod is provided with a downward opening groove, and the groove is provided with a ramp body with an inclined surface facing the sealing cavity, and the ramp body is connected to an elastic component.

[0006] Furthermore, at least one limiting ring is provided on the inner wall of the sealed cavity, and the limiting ring divides the sealed cavity into a plurality of interconnected cavities.

[0007] Furthermore, the piston head is an elastic body, which has a first state of abutting against the limiting ring and a second state of passing through the limiting ring.

[0008] Furthermore, the piston rod has a scale for marking the cumulative volume of the cavity, and the cumulative volume is the sum of the volumes of all cavities on the side of each limiting ring facing the liquid outlet pipe.

[0009] Furthermore, the limiting ring is nested and connected with the spiral rotating handle, and the spiral rotating handle can be spirally rotated relative to the limiting ring and drive the limiting ring to move horizontally when rotating; The limiting ring is fixedly connected to the square block via a connecting rod, and the protruding block is fixedly connected to the square block.

[0010] Furthermore, the device also includes a base, the elastic component includes a shrapnel box and a shrapnel arranged in the shrapnel box, and the inclined body extends downward into the shrapnel box and abuts against the shrapnel.

[0011] Furthermore, the liquid storage box has a bottom wall inclined toward the liquid inlet pipe.

[0012] Due to the adoption of the above technical solution, the beneficial effects of this application are as follows: 1. The feeding assembly of the present application is a three-layer structure, which is respectively an outer cylinder, a rotating cylinder and a sealed cavity from the outside to the inside, and a spiral rotating handle is arranged between the outer cylinder and the rotating cylinder to drive the rotating cylinder to rotate. In the present application, the liquid inlet pipe of the outer cylinder and the first circular hole of the sealed cavity are always aligned, and the liquid outlet pipe and the second circular hole are always aligned, and the liquid inlet pipe and the first circular hole are connected, and the liquid outlet pipe and the second circular hole are connected by rotating the rotating cylinder. Specifically, when the spiral rotating handle is rotated, the friction between the protrusion and the guide groove will drive the rotating cylinder to rotate. When the de-eluent needs to be added to the sealed cavity, the protrusion is located at the initial end of the guide groove, and the liquid inlet hole is aligned with the first circular hole and the liquid inlet pipe; when the de-eluent needs to be added to the chromatography component, the spiral rotating handle is rotated outward, and the spiral rotating handle rotates relative to the limit ring (the limit ring does not rotate), thereby pulling the limit ring to translate outward, and then the square block pulls the protrusion to move along the starting end of the guide groove to the end end, and finally the rotating cylinder rotates. When the protrusion reaches the rear end, the liquid outlet hole rotates to between the liquid outlet pipe and the second circular hole, and cooperates with the piston to input the eluent into the chromatography component.

[0013] 2. The present application can achieve quantitative addition of the de-laundering agent. Specifically, a limiting ring is designed in the sealing cavity to clamp the piston head. When the piston head is clamped with a limiting ring, the operator can clearly feel the resistance increase. The cavity capacity corresponding to the limiting ring is designed on the outside of the piston rod so that the operator can read the volume of the de-laundering agent in the cavity. As a preferred embodiment of the present application, the piston head material is designed to be an elastomer. When the resistance continues to increase, the piston head can be deformed to pass through the limiting ring and enter the next cavity. When it reaches the limiting ring of the next cavity, the cavity volume corresponding to the limiting ring can continue to be read.

[0014] 3. In order to prevent the piston rod from being accidentally touched, the present application designs a bevel and a slot structure to limit the piston rod. When the piston rod moves outward, the resistance is small, and when it is pushed inward, the resistance is limited. This can prevent the piston rod from being accidentally touched when the spiral handle is rotated, resulting in errors in the amount of detergent injected. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The drawings described herein are used to provide further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute improper limitations on the present application.

[0016] Figure 1 A schematic diagram of the overall structure of a device for quantitatively administering a hirudin de-eluent provided in the present application; Figure 2 A schematic diagram of the front cross-section structure of a device for quantitatively administering a hirudin de-eluent provided for the application; Figure 3 for Figure 2 Enlarged view of point A in the middle; Figure 4 It is a schematic diagram of the top cross-section structure of the feeding assembly described in this application; Figure 5 for Figure 4 Enlarged view of point B in the middle; Figure 6 This is an exploded view of the feeding assembly in this application after omitting the outer cylinder; In the attached picture: 1. Liquid storage box; 2. Feeding assembly; 2-1. Outer cylinder; 2-2. Rotating cylinder; 2-3. Sealing chamber; 3. Chromatography assembly; 4. Liquid inlet pipe; 5. Liquid outlet pipe; 6. First circular hole; 7. Second circular hole; 8. Chamber; 9. Driving assembly; 9-1. Threaded rotating handle; 9-2. Limiting ring; 9-3. Square block; 9-4. Bump; 9-5. Connecting rod; 10. Guide groove; 10-1. Starting end; 10-2. End end; 11. Inflow hole; 12. Outflow hole; 13. Piston; 13-1. Piston head; 13-2. Piston rod; 13-3. Slot; 13-4. Inclined body; 14. Elastic assembly; 14-1. Shrapnel box; 14-2. Shrapnel; 15. Limiting ring; 16. Base. DETAILED DESCRIPTION

[0017] Based on the background technology, accurate control and quantitative administration of hirudin de-eluent are crucial to improving the efficiency and effect of the hirudin purification process. Compared with traditional laboratory syringes, the injection of hirudin de-eluent in a closed environment can effectively avoid waste of de-eluent and environmental pollution.

[0018] In order to solve the above problems, the present application provides a device for quantitatively administering hirudin de-eluting agent, including a feeding component 2 and a liquid storage box 1 and a chromatography component 3 respectively connected to the feeding component 2; the chromatography component 3 includes an ion exchange column, a collecting device, a control valve and a monitoring system. The ion exchange column is the core part of the chromatography component 3, which is filled with ion exchange resin for adsorbing and releasing hirudin. : The collecting device is used to collect the eluate containing high-purity hirudin after chromatography. The control valve adjusts the flow rate and pressure of the liquid flowing through the ion exchange column to ensure the stability and efficiency of the chromatography process. Monitoring system: generally includes a pH meter, a conductivity meter, etc., which are used to monitor the properties of the eluate and evaluate the purification effect of hirudin. The chromatography component 3 can be realized based on the existing technology.

[0019] The feeding assembly 2 includes an outer cylinder 2-1, a sealed cavity 2-3, and a rotating cylinder 2-2 disposed between the outer cylinder 2-1 and the sealed cavity 2-3.

[0020] The outer cylinder 2-1 is provided with a liquid inlet pipe 4 and a liquid outlet pipe 5 which are communicated with the liquid storage box 1 and the chromatography component 3 respectively; the sealed cavity 2-3 is provided with a first circular hole 6 and a second circular hole 7 which are aligned with the liquid inlet pipe 4 and the liquid outlet pipe 5 respectively.

[0021] There is a chamber 8 between the outer cylinder 2-1 and the rotating cylinder 2-2, and the chamber 8 is provided with a driving assembly 9 for driving the rotating cylinder 2-2 to rotate. The driving assembly 9 is driven by a threaded rotating handle 9-1 and is connected to the rotating cylinder 2-2 through a limit ring 9-2, a square block 9-3, and a protrusion 9-4 in sequence; the protrusion 9-4 protrudes toward the rotating cylinder 2-2 and the rotating cylinder 2-2 has a guide groove 10 matching the protrusion 9-4, and the guide groove 10 has a starting end 10-1 and an ending end 10-2 and extends spirally from the starting end 10-1 to the ending end 10-2.

[0022] The drum 2-2 has an inlet hole 11 and an outlet hole 12, and the inlet hole 11 and the outlet hole 12 are constructed so that when the protrusion 9-4 is located at the starting end 10-1, the inlet hole 11 is aligned with the first circular hole 6, and when the protrusion 9-4 is located at the tail end 10-2, the outlet hole 12 is aligned with the second circular hole 7.

[0023] The sealing chamber 2-3 is provided with a piston 13 extending from the inside to the outside, and the piston 13 includes a piston 13 head located inside the sealing chamber 2-3 and a piston 13 rod located outside. The end of the piston 13 rod is provided with a downward opening groove 13-3, and the groove 13-3 is provided with a bevel body 13-4 with an inclined surface facing the sealing chamber 2-3, and the bevel body 13-4 is connected to an elastic component 14.

[0024] See attached Figure 1 , Attachment Figure 2 , Attachment Figure 4 and attached Figure 6 , the liquid storage box 1 is located at the top, and it has a degreasing agent inlet. After the degreasing agent is added to the liquid storage box 1, it flows downward to the liquid inlet pipe 4. In the initial state, the spiral rotating handle is located at the innermost side. At this time, the first circular hole 6, the liquid inlet hole and the liquid inlet pipe 4 are the same, and the degreasing agent fills the sealing cavity 2-3. When the spiral rotating handle is spirally rotated to the right, it rotates relative to the limit ring 9-2, but the limit ring 9-2 does not rotate, but moves horizontally to the right under the action of the nested connection, thereby pulling the square block 9-3 and the protrusion 9-4 to move to the right. See the attached Figure 5 The protrusion 9-4 has a tendency to move downward but cannot move, and applies an upward friction force to the rotation to drive the drum 2-2 to rotate counterclockwise. As an example but not a limitation, the protrusion 9-4 can be a cylindrical block or a rectangular block, and it can also be a cylindrical block that can rotate around the central axis of the cylindrical block. As an example but not a limitation, see the attached Figure 4 and attached Figure 6 In order to make the rotation smoother, the present application designs two sets of driving components 9 which are respectively arranged at the symmetrical ends of the limit ring 9-2. Figure 6The two guide grooves 10 shown in the figure are two independent guide grooves 10. The starting end 10-1 of the lower guide groove 10 is blocked by the square block 9-3 and cannot be shown. The other guide groove 10 above shows the end 10-2, and its starting end 10-1 cannot be shown on the back of the picture. Figure 6 In the embodiment, the arc of the guide groove 10 around the drum 2-2 is 120 degrees, that is, after the drum 2-2 rotates 120 degrees, the device switches from the liquid inlet state to the liquid infusion state. In the liquid infusion state, the piston 13 can be pushed to inject the eluent into the chromatography component 3. Figure 3 , a slot 13-3 is provided on the outside of the piston 13 rod, and a bevel 13-4 is provided in the slot 13-3. When the piston 13 rod is pulled outward, the bevel 13-4 limits the piston 13 rod, and at this time, the bevel 13-4 needs to be pressed down by hand. Under normal conditions, the bevel 13-4 can be clamped in the slot 13-3 under the action of the elastic component 14 to avoid accidental touch when turning the spiral turning handle. If necessary, the outer cylinder 2-1 and the cylinder wall on the right side of the sealed chamber 2-3 should be designed with a through hole connected to the outside world to avoid the sealed chamber 2-3 from being unable to complete pressure relief. As an example and not a limitation, the bottom of the liquid outlet pipe 5 can be tilted toward the chromatography component 3 to avoid residual desolvent in the liquid outlet pipe 5.

[0025] As a preferred embodiment of the present application, the inner wall of the sealed cavity 2-3 is provided with at least one limiting ring 15, and the limiting ring 15 divides the sealed cavity 2-3 into a plurality of interconnected cavities. A limiting ring 15 is designed in the sealed cavity 2-3 for clamping the piston 13 head. When the piston 13 head is clamped with a limiting ring 15, the operator can obviously feel the resistance increase. The cavity capacity corresponding to the limiting ring 15 is designed outside the piston 13 rod so that the operator can read the volume of the desolvent in the cavity.

[0026] In a specific embodiment of the above-mentioned implementation mode, the piston 13 head is an elastic body, which has a first state of abutting against the limiting ring 15 and a second state of passing through the limiting ring 15. The material of the piston 13 head is designed to be an elastomer, that is, an elastic material. When the resistance continues to increase, the piston 13 head can be deformed to pass through the limiting ring 15 and enter the next cavity. When it reaches the limiting ring 15 of the next cavity, the cavity volume corresponding to the limiting ring 15 can continue to be read. The process of the piston 13 head abutting against or passing through the limiting ring 15 is easily perceived by the operator, and this structure can be used to determine the position inside the piston 13 head.

[0027] In a preferred embodiment of the above embodiment, the piston 13 rod has a scale marking the cumulative volume of the cavity, and the cumulative volume is the sum of the volumes of all cavities on the side of each limiting ring 15 facing the liquid outlet pipe 5. The cumulative volume can be marked by measuring the volume on the left side of the limiting ring 15 before designing the cavity.

[0028] As a preferred embodiment of the present application, the limit ring 9-2 is nested and connected with the spiral rotating handle, and the spiral rotating handle can be spirally rotated relative to the limit ring 9-2 and drive the limit ring 9-2 to move horizontally when rotating; The limiting ring 9-2 is fixedly connected to the square block 9-3 via a connecting rod 9-5, and the protrusion 9-4 is fixedly connected to the square block 9-3.

[0029] As a preferred embodiment of the present application, the device further includes a base 16, the elastic component 14 includes a spring 14-2 box 14-1 and a spring 14-2 disposed in the spring 14-2 box 14-1, and the inclined surface 13-4 extends downward into the spring 14-2 box 14-1 and abuts against the spring 14-2. The spring 14-2 is used to keep the inclined surface 13-4 in an upward trend and limit the piston 13 rod to the left to avoid accidental touch. It should be noted that a bracket for supporting the feeding component 2, the liquid storage box 1 and the chromatography component 3 is also provided on the base 16.

[0030] As a preferred embodiment of the present application, the liquid storage box 1 has a bottom wall inclined toward the liquid inlet pipe 4. This is to ensure that the de-laundering agent flows into the feeding assembly 2 smoothly.

[0031] Anything not described in this application can be achieved by adopting or drawing on existing technologies.

[0032] The above is only an embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various changes and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the scope of the claims of the present application.

Claims

1. A device for quantitatively administering a hirudin de-eluent, comprising a feeding assembly and a liquid storage box and a chromatography assembly respectively connected to the feeding assembly; characterized in that: The feeding assembly comprises an outer cylinder, a sealing cavity and a rotating cylinder arranged between the outer cylinder and the sealing cavity; The outer cylinder is provided with a liquid inlet pipe and a liquid outlet pipe respectively communicating with the liquid storage box and the chromatography component; the sealed cavity is provided with a first circular hole and a second circular hole respectively aligned with the liquid inlet pipe and the liquid outlet pipe; A chamber is provided between the outer cylinder and the rotating cylinder, and the chamber is provided with a driving assembly for driving the rotating cylinder to rotate, the driving assembly is driven by a threaded rotating handle and is connected to the rotating cylinder through a limit ring, a square block, and a protrusion in sequence; the protrusion protrudes toward the rotating cylinder and the rotating cylinder has a guide groove matched with the protrusion, the guide groove has a starting end and a tail end and spirally extends from the starting end to the tail end; The drum has an inflow hole and an outflow hole, and the inflow hole and the outflow hole are configured such that when the protrusion is located at the starting end, the inflow hole is aligned with the first circular hole, and when the protrusion is located at the trailing end, the outflow hole is aligned with the second circular hole; The sealing cavity is provided with a piston extending from the inside to the outside, and the piston includes a piston head located inside the sealing cavity and a piston rod located outside. The end of the piston rod is provided with a downward opening groove, and the groove is provided with a ramp body with an inclined surface facing the sealing cavity, and the ramp body is connected to an elastic component.

2. A device for quantitatively administering a leech de-eluent according to claim 1, characterized in that: The inner wall of the sealed cavity is provided with at least one limiting ring, and the limiting ring divides the sealed cavity into a plurality of interconnected cavities.

3. A device for quantitatively administering a leech de-eluent according to claim 2, characterized in that: The piston head is an elastic body, and has a first state of abutting against the limiting ring and a second state of passing through the limiting ring.

4. A device for quantitatively administering a leech de-eluent according to claim 3, characterized in that: The piston rod has a scale for marking the cumulative volume of the cavity, and the cumulative volume is the sum of the volumes of all cavities on the side of each limiting ring facing the liquid outlet pipe.

5. The device for quantitatively administering a leech de-eluent according to claim 1, characterized in that: The limiting ring is nested with the spiral rotating handle, and the spiral rotating handle can be spirally rotated relative to the limiting ring and drive the limiting ring to move horizontally when rotating; The limiting ring is fixedly connected to the square block via a connecting rod, and the protruding block is fixedly connected to the square block.

6. The device for quantitatively administering a leech de-eluent according to claim 1, characterized in that: The device also includes a base, the elastic component includes a shrapnel box and a shrapnel arranged in the shrapnel box, and the inclined surface body extends downward into the shrapnel box and abuts against the shrapnel.

7. The device for quantitatively administering a leech de-eluent according to claim 1, characterized in that: The liquid storage box has a bottom wall inclined toward the liquid inlet pipe.

Images