A method for cultivating selenium-enriched liquid fertilizer and selenium-enriched coriander.

Abstract

This invention discloses a selenium-enriched liquid fertilizer and a method for cultivating selenium-enriched coriander, belonging to the field of selenium-enriched crop cultivation technology. The selenium-enriched liquid fertilizer includes a basic selenium-enriched liquid fertilizer and a growth regulator. By using the selenium-enriched basic liquid fertilizer and the growth regulator in combination and spraying them at specific growth stages, the selenium content, reducing sugar content, total flavonoid content, and hydroxyl radical scavenging rate in coriander are significantly increased, resulting in coriander rich in nutrients and with a significantly high selenium content. Furthermore, the growth regulator and selenium-enriched liquid fertilizer of this application provide new ideas for the cultivation of spice crops, including coriander.

Description

Technical Field

[0001] This invention belongs to the field of selenium-enriched crop cultivation technology, specifically relating to a selenium-enriched liquid fertilizer and a method for cultivating selenium-enriched coriander. Background Technology

[0002] Cilantro is another name for several plants, such as coriander, arugula, and cilantro. Coriander, also known as salted coriander, is an annual herb belonging to the genus *Coriandrum* in the family Apiaceae. The tender stems and fresh leaves of cilantro have a distinctive aroma and are often used as a garnish and flavor enhancer in dishes. Cilantro is rich in active nutritional components and possesses good edible and medicinal value, including antibacterial, antioxidant, anti-anxiety, and anti-cancer properties.

[0003] Selenium is a non-metallic chemical element that is a gray or red crystal or a red amorphous powder. It is an essential trace element for human health, playing important biological functions in many areas, including anti-oxidation and anti-aging, lowering blood pressure, regulating immunity, preventing and fighting cancer, and preventing arteriosclerosis. It is also a component of some enzymes, including glutathione peroxidase, iodothyronine deiodase, and thioredoxin reductase. Selenium can protect cells from the harmful effects of free radicals formed during oxidation. The selenium in the human body mainly comes from plants and animals, with organic selenium from plants being more easily absorbed and utilized by the body than organic selenium from animals. Therefore, organic selenium from plants is one of the main sources of selenium supplementation for the human body. With the development of society, more and more people are paying attention to their health and realizing the importance of selenium supplementation. Increasing the selenium content of plants through various organic conversion methods is the safest and most effective way to increase selenium intake for humans and animals.

[0004] Currently, the main method for producing selenium-enriched coriander involves adding a certain amount of sodium selenite as a base fertilizer to farmyard manure. However, due to the influence of the physical and chemical properties of the planting soil, the selenium content of coriander produced by this method is unstable and very low. In addition, directly applying sodium selenite to the soil can easily cause soil selenium pollution, and the efficiency of plant absorption and utilization of selenium is also low. Therefore, this is an environmentally unfriendly selenium-enriched plant cultivation technique.

[0005] Based on this, we propose a method for cultivating selenium-enriched liquid fertilizer and selenium-enriched coriander, hoping to address the shortcomings of existing technologies. Summary of the Invention

[0006] The purpose of this invention is to address existing problems by providing a method for cultivating selenium-enriched liquid fertilizer and selenium-enriched coriander.

[0007] This invention is achieved through the following technical solution:

[0008] A selenium-enriched liquid fertilizer, comprising a basic selenium-enriched liquid fertilizer and a growth regulator.

[0009] Furthermore, the preparation of the basic selenium-enriched liquid fertilizer includes the following steps:

[0010] (1) After drying corn cobs, rice straw, purple sweet potato vines and kudzu vines to constant weight, they are placed in a plant pulverizer and pulverized according to the mass ratio of corn cobs: rice straw: purple sweet potato vines: kudzu vines = 1:2~3:0.5~0.7:0.3~0.5 to obtain mixed powder for later use;

[0011] (2) Immerse the obtained mixed powder in the treatment agent, and after constant temperature ultrasonic stirring for 3-5 hours, place it in a high temperature and high pressure cooker for high temperature and high pressure treatment. Then filter, collect the filtrate and concentrate it to 40-60% of the original volume to obtain crop concentrate for later use.

[0012] (3) Mix the crop concentrate and sterile water at a volume ratio of 1:8 to 12, add manganese sulfate, magnesium sulfate and sodium chloride, stir and mix well, sterilize, cool to room temperature, inoculate with Bacillus subtilis, culture in a shaker for 3 to 5 hours, add Na2SeO3, continue to culture for 2 to 4 days, and then filter to obtain basic selenium-enriched liquid fertilizer.

[0013] Further, the components and corresponding weight percentages of the treatment agent described in step (2) are as follows: fulvic acid 0.7-1.3%, lanthanum nitrate 1-2%, cerium nitrate 0.8-1.4%, phosphate ester amphoteric surfactant 7-10%, and the remainder is pure water.

[0014] Furthermore, the constant temperature mentioned in step (2) is 50 to 70°C.

[0015] Furthermore, the high-temperature and high-pressure treatment in step (2) is carried out at a temperature of 100-120°C and a pressure of 0.2-0.5 MPa.

[0016] Further, the final concentrations of manganese sulfate, magnesium sulfate and sodium chloride mentioned in step (3) are 0.02-0.03 mg / mL, 0.1-0.2 mg / mL and 0.1-0.3 mg / mL, respectively.

[0017] Furthermore, the inoculation amount of Bacillus subtilis in step (3) is 2-3%;

[0018] The incubation temperature is 35–39℃, and the shaking speed is 120–150 rpm.

[0019] Further, the preparation method of the growth regulator is as follows: under ultrasonic conditions, brown algae oligosaccharide is added to deionized water, and ultrasonically stirred at 60-70℃ for 30-50 min. Then, while stirring, glycidyltrimethylammonium chloride is added. After thorough mixing, the temperature is raised to 80-90℃ and reacted for 20-30 min. Then, the temperature is lowered to 50-60℃ and deionized water and amino acid chelate are added. The mixture is stirred at a constant temperature for 30-40 min and then cooled to room temperature. Glutathione and silk fibroin are then added, and stirring is continued for 1-2 h.

[0020] Furthermore, in the growth regulator described in 1L, the amounts of each raw material added are as follows: 0.6-1g of fucoidan, 1-1.5g of glycidyltrimethylammonium chloride, 0.8-1.2g of amino acid chelate, 2-3g of glutathione, and 8-12g of silk fibroin.

[0021] A method for cultivating selenium-enriched coriander includes the following steps:

[0022] (1) Apply base fertilizer to the soil before planting;

[0023] (2) Apply the above-mentioned basic selenium-enriched liquid fertilizer to the roots 4-5 days after the coriander seedlings emerge;

[0024] (3) After the coriander seedlings grow to 8-12cm in height, mix the above-mentioned basic selenium-enriched liquid fertilizer and growth regulator in a ratio of 70-90:1 and spray it on the coriander leaves.

[0025] The present invention has the following advantages over the prior art:

[0026] This invention provides a method for cultivating selenium-enriched liquid fertilizer and selenium-enriched coriander. By combining selenium-enriched liquid fertilizer with growth regulators and spraying it during specific growth stages, the selenium content, reducing sugar content, total flavonoid content, and hydroxyl radical scavenging rate in coriander are significantly increased. Coriander is rich in nutrients and has a significant selenium content.

[0027] Firstly, since plants primarily absorb selenium through their roots, mainly by increasing the contact area with the soil via root hairs to enhance absorption efficiency, selenium, once absorbed into plant cells, needs to be transported to organelles such as chloroplasts for biotransformation. Root absorption is faster than leaf transformation. Furthermore, the biotransformation process can affect the plant's utilization efficiency and toxicity of selenium. Therefore, to improve the effective utilization rate of selenium, this application adjusts the fertilization method, employing different fertilization approaches at different growth stages based on the growth characteristics of coriander, thereby increasing selenium content and bioavailability. This aims to meet people's demand for selenium and increase the added value of coriander.

[0028] Secondly, corn cobs, rice straw, purple sweet potato vines, and kudzu vines are processed in a specific ratio, then sterilized with manganese sulfate, magnesium sulfate, and sodium chloride. After inoculation with Bacillus subtilis and culturing in a shaker for 3-5 hours, Na2SeO3 is added. The combination of rare earth compounds and nutrients intensifies the logarithmic growth phase of Bacillus subtilis, promotes vigorous metabolism, and shortens the plateau phase. During its growth, Bacillus subtilis absorbs and utilizes selenium, converting it into organic selenium through a series of organic combinations (with proteins, polysaccharides, etc.). It also metabolizes inorganic selenium compounds with low bioavailability into bioavailable organic selenium forms such as selenocysteine ​​or selenomethionine, resulting in a high selenium conversion rate. Furthermore, the basic selenium-enriched liquid fertilizer of this application can improve cell permeability, thereby enhancing the absorption and conversion of effective components by the roots. In this growth regulator, the combination of alginic oligosaccharides, glycidyltrimethylammonium chloride, amino acid chelates, glutathione, silk fibroin, and basic selenium-enriched liquid fertilizer enhances the fertilizer's adhesion to leaves, prolongs its duration of action, and improves its effectiveness. Furthermore, the addition of these growth regulators stimulates leaves, altering the activity of enzymes such as ATP sulfatedase (APS) and selenocysteine ​​methyltransferase (SMT) during selenium metabolism, promoting selenium conversion in chloroplasts, and facilitating the absorption and conversion of active ingredients in the liquid fertilizer by leaves. This results in the enrichment of organic selenium without toxicity to plants.

[0029] Finally, the growth regulators and selenium-enriched liquid fertilizers of this application provide new ideas for the cultivation of spice crops, including coriander. Attached Figure Description

[0030] Figure 1 The effect of changes in the amount of selenium-enriched liquid fertilizer applied to the roots on the selenium content in coriander.

[0031] Figure 2 The effect of varying application rates of foliar selenium-enriched liquid fertilizer on the selenium content in coriander. Detailed Implementation

[0032] To further explain the present invention, the following specific embodiments are described.

[0033] Example 1

[0034] A method for cultivating selenium-enriched coriander includes the following steps:

[0035] (1) Apply base fertilizer to the soil before planting;

[0036] (2) Apply the above-mentioned basic selenium-enriched liquid fertilizer to the roots 4 days after the coriander seedlings emerge.

[0037] (3) After the coriander seedlings grow to 8cm in height, mix the above-mentioned basic selenium-enriched liquid fertilizer and growth regulator in a ratio of 70:1 and spray it on the coriander leaves.

[0038] The base fertilizer is well-rotted organic fertilizer, and the application rate is 800 kg / mu;

[0039] The application rate of the basic selenium-enriched liquid fertilizer is 40L / mu;

[0040] The application rate for foliar spraying is 20L / mu;

[0041] The preparation of the basic selenium-enriched liquid fertilizer includes the following steps:

[0042] (1) After drying the corn cobs, rice straw, purple sweet potato vines and kudzu vines to constant weight, they were placed in a plant pulverizer and pulverized in a mass ratio of corn cobs: rice straw: purple sweet potato vines: kudzu vines = 1:2:0.5:0.3 to obtain mixed powder for later use;

[0043] (2) The obtained mixed powder is immersed in the treatment agent, and ultrasonically stirred at 50°C for 3 hours. Then it is placed in a high-temperature and high-pressure cooker at 100°C and 0.2MPa for high-temperature and high-pressure treatment. Then it is filtered, and the filtrate is collected and concentrated to 40% of the original volume to obtain crop concentrate for use.

[0044] The components and their corresponding weight percentages in the treatment agent are as follows: fulvic acid 0.7%, lanthanum nitrate 1%, cerium nitrate 0.8%, phosphate ester amphoteric surfactant 7%, and the balance being pure water;

[0045] (3) After mixing the crop concentrate and sterile water at a volume ratio of 1:8, add manganese sulfate, magnesium sulfate and sodium chloride, stir and mix well, then sterilize. After cooling to room temperature, inoculate with Bacillus subtilis at an inoculation amount of 2%. Incubate in a shaker at 35°C and 120 rpm for 3 hours, then add Na2SeO3 and continue to incubate for 2 days. After filtration, obtain the basic selenium-enriched liquid fertilizer.

[0046] The final concentrations of manganese sulfate, magnesium sulfate, and sodium chloride are 0.02 mg / mL, 0.1 mg / mL, and 0.1 mg / mL, respectively.

[0047] The preparation method of the growth regulator is as follows: under ultrasonic conditions, brown algae oligosaccharide is added to deionized water, ultrasonically stirred at 60°C for 30 min, glycidyl trimethylammonium chloride is added while stirring, and after thorough mixing, the temperature is raised to 80°C and reacted for 20 min, then cooled to 50°C and deionized water and amino acid chelate are added, and the mixture is stirred at constant temperature for 30 min, then cooled to room temperature, and then glutathione and silk fibroin are added, and stirring is continued for 1 h.

[0048] The addition amounts of each raw material in the growth regulator described in 1L are as follows: 0.6g of fucoidan, 1g of glycidyl trimethylammonium chloride, 0.8g of amino acid chelate, 2-3g of glutathione, and 8g of silk fibroin.

[0049] Example 2

[0050] A method for cultivating selenium-enriched coriander includes the following steps:

[0051] (1) Apply base fertilizer to the soil before planting;

[0052] (2) Apply the above-mentioned basic selenium-enriched liquid fertilizer to the roots 4 days after the coriander seedlings emerge.

[0053] (3) After the coriander seedlings grow to 10cm in height, mix the above-mentioned basic selenium-enriched liquid fertilizer and growth regulator in a ratio of 80:1 and spray it on the coriander leaves.

[0054] The base fertilizer is well-rotted organic fertilizer, and the application rate is 800 kg / mu;

[0055] The application rate of the basic selenium-enriched liquid fertilizer is 45L / mu;

[0056] The application rate for foliar spraying is 30L / mu;

[0057] The preparation of the basic selenium-enriched liquid fertilizer includes the following steps:

[0058] (1) After drying the corn cobs, rice straw, purple sweet potato vines and kudzu vines to constant weight, they were placed in a plant pulverizer and pulverized in a mass ratio of corn cobs: rice straw: purple sweet potato vines: kudzu vines = 1:2.5:0.6:0.4 to obtain mixed powder for later use.

[0059] (2) The obtained mixed powder was immersed in the treatment agent, and ultrasonically stirred at 60°C for 4 hours. Then it was placed in a high-temperature and high-pressure cooker at 110°C and 0.35MPa for high-temperature and high-pressure treatment. After filtration, the filtrate was collected and concentrated to 50% of the original volume to obtain crop concentrate for use.

[0060] The components and their corresponding weight percentages in the treatment agent are as follows: fulvic acid 1%, lanthanum nitrate 1.5%, cerium nitrate 1.1%, phosphate ester amphoteric surfactant 8%, and the balance being pure water;

[0061] (3) Mix the crop concentrate and sterile water at a volume ratio of 1:10, add manganese sulfate, magnesium sulfate and sodium chloride, stir and mix well, then sterilize, cool to room temperature, inoculate with Bacillus subtilis, the inoculation amount of Bacillus subtilis is 2.5%, culture in a shaker at 37℃ and 130rpm for 4h, add Na2SeO3, continue to culture for 3 days, and then filter to obtain basic selenium-enriched liquid fertilizer.

[0062] The final concentrations of manganese sulfate, magnesium sulfate, and sodium chloride are 0.025 mg / mL, 0.15 mg / mL, and 0.2 mg / mL, respectively.

[0063] The preparation method of the growth regulator is as follows: under ultrasonic conditions, brown algae oligosaccharide is added to deionized water, ultrasonically stirred at 65°C for 40 min, glycidyl trimethylammonium chloride is added while stirring, and after thorough mixing, the temperature is raised to 85°C and reacted for 25 min, then cooled to 55°C and deionized water and amino acid chelate are added, and the mixture is stirred at a constant temperature for 35 min, then cooled to room temperature, and then glutathione and silk fibroin are added, and stirring is continued for 1.5 h.

[0064] The addition amounts of each raw material in the growth regulator described in 1L are as follows: 0.8g of fucoidan, 1.2g of glycidyl trimethylammonium chloride, 1g of amino acid chelate, 2.5g of glutathione, and 10g of silk fibroin.

[0065] Example 3

[0066] A method for cultivating selenium-enriched coriander includes the following steps:

[0067] (1) Apply base fertilizer to the soil before planting;

[0068] (2) Five days after the coriander seedlings emerge, apply the above-mentioned basic selenium-enriched liquid fertilizer to the roots.

[0069] (3) After the coriander seedlings grow to 12cm in height, mix the above-mentioned basic selenium-enriched liquid fertilizer and growth regulator in a ratio of 90:1 and spray it on the coriander leaves.

[0070] The base fertilizer is well-rotted organic fertilizer, and the application rate is 800 kg / mu;

[0071] The application rate of the basic selenium-enriched liquid fertilizer is 50L / mu;

[0072] The application rate for foliar spraying is 40L / mu;

[0073] The preparation of the basic selenium-enriched liquid fertilizer includes the following steps:

[0074] (1) After drying the corn cobs, rice straw, purple sweet potato vines and kudzu vines to constant weight, they are placed in a plant pulverizer and pulverized according to the mass ratio of corn cobs: rice straw: purple sweet potato vines: kudzu vines = 1:3:0.7:0.5 to obtain mixed powder for later use;

[0075] (2) The obtained mixed powder was immersed in the treatment agent, and ultrasonically stirred at 70°C for 5 hours. Then it was placed in a high-temperature and high-pressure cooker at 120°C and 0.5MPa for high-temperature and high-pressure treatment. After filtration, the filtrate was collected and concentrated to 60% of the original volume to obtain crop concentrate for use.

[0076] The components and their corresponding weight percentages in the treatment agent are as follows: fulvic acid 1.3%, lanthanum nitrate 2%, cerium nitrate 1.4%, phosphate ester amphoteric surfactant 10%, and the balance being pure water;

[0077] (3) Mix the crop concentrate and sterile water at a volume ratio of 1:12, add manganese sulfate, magnesium sulfate and sodium chloride, stir and mix well, then sterilize, cool to room temperature, inoculate with Bacillus subtilis at an inoculation amount of 3%, culture in a shaker at 39°C and 150 rpm for 5 h, add Na2SeO3, continue to culture for 4 days, and then filter to obtain basic selenium-enriched liquid fertilizer.

[0078] The final concentrations of manganese sulfate, magnesium sulfate, and sodium chloride are 0.03 mg / mL, 0.2 mg / mL, and 0.3 mg / mL, respectively.

[0079] The preparation method of the growth regulator is as follows: under ultrasonic conditions, brown algae oligosaccharide is added to deionized water, ultrasonically stirred at 70°C for 50 min, glycidyltrimethylammonium chloride is added while stirring, and after thorough mixing, the temperature is raised to 90°C and reacted for 30 min, then cooled to 60°C and deionized water and amino acid chelate are added, and the mixture is stirred at a constant temperature for 40 min, then cooled to room temperature, and then glutathione and silk fibroin are added, and stirring is continued for 2 h.

[0080] The addition amounts of each raw material in the growth regulator described in 1L are as follows: 1g of fucoidan, 1.5g of glycidyl trimethylammonium chloride, 1.2g of amino acid chelate, 3g of glutathione, and 12g of silk fibroin.

[0081] Comparative Example 1

[0082] A method for cultivating selenium-enriched coriander includes the following steps:

[0083] (1) Apply base fertilizer to the soil before planting;

[0084] (2) Apply the above-mentioned basic selenium-enriched liquid fertilizer to the roots 4 days after the coriander seedlings emerge.

[0085] (3) After the coriander seedlings grow to 10cm in height, spray the coriander leaves with the above-mentioned basic selenium-enriched liquid fertilizer.

[0086] The base fertilizer is well-rotted organic fertilizer, and the application rate is 800 kg / mu;

[0087] The application rate of the basic selenium-enriched liquid fertilizer is 45L / mu;

[0088] The application rate for foliar spraying is 30L / mu;

[0089] The preparation of the basic selenium-enriched liquid fertilizer includes the following steps:

[0090] (1) After drying the corn cobs, rice straw, purple sweet potato vines and kudzu vines to constant weight, they were placed in a plant pulverizer and pulverized in a mass ratio of corn cobs: rice straw: purple sweet potato vines: kudzu vines = 1:2.5:0.6:0.4 to obtain mixed powder for later use.

[0091] (2) The obtained mixed powder was immersed in the treatment agent, and ultrasonically stirred at 60°C for 4 hours. Then it was placed in a high-temperature and high-pressure cooker at 110°C and 0.35MPa for high-temperature and high-pressure treatment. After filtration, the filtrate was collected and concentrated to 50% of the original volume to obtain crop concentrate for use.

[0092] The components and their corresponding weight percentages in the treatment agent are as follows: fulvic acid 1%, lanthanum nitrate 1.5%, cerium nitrate 1.1%, phosphate ester amphoteric surfactant 8%, and the balance being pure water;

[0093] (3) Mix the crop concentrate and sterile water at a volume ratio of 1:10, add manganese sulfate, magnesium sulfate and sodium chloride, stir and mix well, then sterilize, cool to room temperature, inoculate with Bacillus subtilis, the inoculation amount of Bacillus subtilis is 2.5%, culture in a shaker at 37℃ and 130rpm for 4h, add Na2SeO3, continue to culture for 3 days, and then filter to obtain basic selenium-enriched liquid fertilizer.

[0094] The final concentrations of manganese sulfate, magnesium sulfate, and sodium chloride are 0.025 mg / mL, 0.15 mg / mL, and 0.2 mg / mL, respectively.

[0095] Comparative Example 2

[0096] A method for cultivating selenium-enriched coriander includes the following steps:

[0097] (1) Apply base fertilizer to the soil before planting;

[0098] (2) Four days after the coriander seedlings emerge, mix the above-mentioned basic selenium-enriched liquid fertilizer and growth regulator at a ratio of 80:1 and apply to the roots.

[0099] (3) After the coriander seedlings grow to 10cm in height, mix the above-mentioned basic selenium-enriched liquid fertilizer and growth regulator in a ratio of 80:1 and spray it on the coriander leaves.

[0100] The base fertilizer is well-rotted organic fertilizer, and the application rate is 800 kg / mu;

[0101] The application rate of the basic selenium-enriched liquid fertilizer is 45L / mu;

[0102] The application rate for foliar spraying is 30L / mu;

[0103] The preparation of the basic selenium-enriched liquid fertilizer includes the following steps:

[0104] (1) After drying the corn cobs, rice straw, purple sweet potato vines and kudzu vines to constant weight, they were placed in a plant pulverizer and pulverized in a mass ratio of corn cobs: rice straw: purple sweet potato vines: kudzu vines = 1:2.5:0.6:0.4 to obtain mixed powder for later use.

[0105] (2) The obtained mixed powder was immersed in the treatment agent, and ultrasonically stirred at 60°C for 4 hours. Then it was placed in a high-temperature and high-pressure cooker at 110°C and 0.35MPa for high-temperature and high-pressure treatment. After filtration, the filtrate was collected and concentrated to 50% of the original volume to obtain crop concentrate for use.

[0106] The components and their corresponding weight percentages in the treatment agent are as follows: fulvic acid 1%, lanthanum nitrate 1.5%, cerium nitrate 1.1%, phosphate ester amphoteric surfactant 8%, and the balance being pure water;

[0107] (3) Mix the crop concentrate and sterile water at a volume ratio of 1:10, add manganese sulfate, magnesium sulfate and sodium chloride, stir and mix well, then sterilize, cool to room temperature, inoculate with Bacillus subtilis, the inoculation amount of Bacillus subtilis is 2.5%, culture in a shaker at 37℃ and 130rpm for 4h, add Na2SeO3, continue to culture for 3 days, and then filter to obtain basic selenium-enriched liquid fertilizer.

[0108] The final concentrations of manganese sulfate, magnesium sulfate, and sodium chloride are 0.025 mg / mL, 0.15 mg / mL, and 0.2 mg / mL, respectively.

[0109] The preparation method of the growth regulator is as follows: under ultrasonic conditions, brown algae oligosaccharide is added to deionized water, ultrasonically stirred at 65°C for 40 min, glycidyl trimethylammonium chloride is added while stirring, and after thorough mixing, the temperature is raised to 85°C and reacted for 25 min, then cooled to 55°C and deionized water and amino acid chelate are added, and the mixture is stirred at a constant temperature for 35 min, then cooled to room temperature, and then glutathione and silk fibroin are added, and stirring is continued for 1.5 h.

[0110] The addition amounts of each raw material in the growth regulator described in 1L are as follows: 0.8g of fucoidan, 1.2g of glycidyl trimethylammonium chloride, 1g of amino acid chelate, 2.5g of glutathione, and 10g of silk fibroin.

[0111] Comparative Example 3

[0112] A method for cultivating selenium-enriched coriander includes the following steps:

[0113] (1) Apply well-rotted organic fertilizer to the soil before planting;

[0114] (2) Four days after the coriander seedlings emerge, apply the above-mentioned well-rotted organic fertilizer to the roots.

[0115] The amount of fertilizer applied before planting is 800 kg / mu; the amount of fertilizer applied through root application is 45 L / mu.

[0116] The preparation of the basic selenium-enriched liquid fertilizer includes the following steps:

[0117] (1) After drying the corn cobs, rice straw, purple sweet potato vines and kudzu vines to constant weight, they were placed in a plant pulverizer and pulverized in a mass ratio of corn cobs: rice straw: purple sweet potato vines: kudzu vines = 1:2.5:0.6:0.4 to obtain mixed powder for later use.

[0118] (2) The obtained mixed powder was immersed in the treatment agent, and ultrasonically stirred at 60°C for 4 hours. Then it was placed in a high-temperature and high-pressure cooker at 110°C and 0.35MPa for high-temperature and high-pressure treatment. After filtration, the filtrate was collected and concentrated to 50% of the original volume to obtain crop concentrate for use.

[0119] The components and their corresponding weight percentages in the treatment agent are as follows: fulvic acid 1%, lanthanum nitrate 1.5%, cerium nitrate 1.1%, phosphate ester amphoteric surfactant 8%, and the balance being pure water;

[0120] (3) Mix the crop concentrate and sterile water at a volume ratio of 1:10, add manganese sulfate, magnesium sulfate and sodium chloride, stir and mix well, then sterilize, cool to room temperature, inoculate with Bacillus subtilis, the inoculation amount of Bacillus subtilis is 2.5%, culture in a shaker at 37℃ and 130rpm for 4h, add Na2SeO3, continue to culture for 3 days, and then filter to obtain basic selenium-enriched liquid fertilizer.

[0121] The final concentrations of manganese sulfate, magnesium sulfate, and sodium chloride are 0.025 mg / mL, 0.15 mg / mL, and 0.2 mg / mL, respectively.

[0122] The preparation method of the growth regulator is as follows: under ultrasonic conditions, brown algae oligosaccharide is added to deionized water, ultrasonically stirred at 65°C for 40 min, glycidyl trimethylammonium chloride is added while stirring, and after thorough mixing, the temperature is raised to 85°C and reacted for 25 min, then cooled to 55°C and deionized water and amino acid chelate are added, and the mixture is stirred at a constant temperature for 35 min, then cooled to room temperature, and then glutathione and silk fibroin are added, and stirring is continued for 1.5 h.

[0123] The addition amounts of each raw material in the growth regulator described in 1L are as follows: 0.8g of fucoidan, 1.2g of glycidyl trimethylammonium chloride, 1g of amino acid chelate, 2.5g of glutathione, and 10g of silk fibroin.

[0124] test

[0125] The coriander samples from Examples 1-3 and Comparative Examples 1-3 were washed with ultrapure water, dried in an oven to constant weight, pulverized with a plant pulverizer, and passed through a 200-mesh sieve for later use.

[0126] 1. Determination of selenium content

[0127] Weigh 0.5 g of powdered sample into a 50 mL flask using an electronic balance, add 15 mL of mixed acid (HNO3:HClO4 = 2:1), and digest for 24 h. Heat on a hot plate until the sample solution is approximately 1 mL, remove and cool. Then add 10 mL of concentrated HCl, heat again until the sample solution is approximately 1 mL, and remove and cool. Add a small amount of ultrapure water to the digestion solution, sonicate until the solution is clear, transfer to a 50 mL volumetric flask, add 12.5 mL of concentrated HCl, and then dilute to the mark with ultrapure water for analysis.

[0128] 2. Determination of reducing sugar content

[0129] According to the first method—direct titration method—of GB 5009.7-2016 "National Food Safety Standard: Determination of Reducing Sugars in Food".

[0130] 3. Determination of protein content

[0131] The protein content in dried coriander products was determined using the Coomassie brilliant blue method.

[0132] 4. Determination of Vitamin C Content

[0133] The determination was performed according to GB / T 6195-1986 "Determination of Vitamin C Content in Fruits and Vegetables (2,6-Dichlorophenolindophenol Titration Method)".

[0134] 5. Determination of total flavonoid content

[0135] The determination was performed according to the method in DB43 / T 476-2009 "Determination of total flavonoids in plant-derived foods".

[0136] 6. Determination of hydroxyl radical scavenging ability

[0137] Using a hydroxyl radical assay kit, an accurate measurement was performed at a wavelength of 550 nm using a UV spectrophotometer, strictly following the instructions.

[0138] The test results are shown in Table 1 below.

[0139] Table 1

[0140]

[0141] As can be seen from the experimental data in Table 1 above, the coriander grown by the methods in Examples 1 to 3 is superior to the comparative example in terms of selenium content, reducing sugar content, total flavonoid content and hydroxyl radical scavenging rate. The coriander is rich in nutrients and has a significant selenium content.

[0142] A comparison of Comparative Example 1 and Example 2 shows that the addition of growth regulators in the final foliar spraying step can increase fertilizer efficiency and help accumulate nutrients.

[0143] Comparing Comparative Example 2 with Example 2, it can be seen that adding growth regulators at the root application stage in step (2) has no significant effect on the content of nutrients, and may even reduce the content of some nutrients. This indicates that the timing of adding growth regulators is very important, and adding them at the right time has a significant effect on the content of nutrients in coriander.

[0144] Based on the above experiments, taking Example 2 as a benchmark, the amount of basic selenium-enriched liquid fertilizer applied in step (2) and the amount of foliar spraying in step (3) were changed respectively, and the changes in selenium content with the increase of fertilizer amount were measured.

[0145] The results are as follows Figure 1 and Figure 2 As shown.

[0146] Depend on Figure 1 It can be seen that after the coriander grows to 10cm in height, the above-mentioned basic selenium-enriched liquid fertilizer and growth regulator are mixed at a ratio of 80:1 and sprayed on the coriander leaves at a rate of 30L / mu. The amount of basic selenium-enriched liquid fertilizer in step (2) is changed. It is found that as the amount of fertilizer increases, the selenium content in coriander first increases and then decreases. This indicates that the application of the selenium-enriched liquid fertilizer in this application has a certain dose effect. In addition, it was found that simply spraying the leaves without applying to the roots does not significantly increase the selenium content.

[0147] Depend on Figure 2 It can be seen that, 4 days after the coriander seedlings emerge, the basic selenium-enriched liquid fertilizer was applied to the roots at a rate of 45 L / mu. The amount of foliar spraying after mixing the basic selenium-enriched liquid fertilizer and the growth regulator in step (3) was changed. It was found that as the amount of spraying increased, the selenium content in the coriander also showed a certain increase and then decrease. It also showed a certain dose effect. It can be seen that the planting method of this application, the root application in step (2) and the foliar fertilization in step (3) have a certain synergistic effect. The two work together to significantly increase the selenium content.

[0148] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A method of growing selenium-enriched coriander, characterized in that, It comprises the following steps: (1) applying base fertilizer to the soil before planting; (2) after the coriander seedlings emerge for 4-5 days, root application is performed with the base selenium-rich liquid fertilizer; (3) after the coriander seedlings grow to a height of 8-12 cm, the base selenium-rich liquid fertilizer and the growth regulator are mixed at a ratio of 70-90:1 and then foliar spraying is performed on the coriander; The preparation method of the base selenium-rich liquid fertilizer comprises the following steps: 1) corn cob, rice straw, purple potato vine and kudzu vine are dried to constant weight, respectively, and then are crushed in a plant crusher at a mass ratio of corn cob:rice straw:purple potato vine:kudzu vine=1:2-3:0.5-0.7:0.3-0.5 to obtain a mixed powder for later use; 2) the obtained mixed powder is immersed in a treatment agent, ultrasonically stirred and treated at a constant temperature for 3-5 h, then is placed in a high-temperature and high-pressure pot for high-temperature and high-pressure treatment, and then is filtered, concentrated to 40-60% of the original volume, and collected to obtain a crop concentrate for later use; 3) the crop concentrate and sterile water are mixed at a volume ratio of 1:8-12, then manganese sulfate, magnesium sulfate and sodium chloride are added and stirred to mix uniformly, and then sterilization is performed, and after cooling to room temperature, Bacillus subtilis is inoculated, cultured in a shaking bed for 3-5 h, then Na2SeO3 is added, and the culture is continued for 2-4 days, and then filtration is performed to obtain the base selenium-rich liquid fertilizer; The preparation method of the growth regulator is that brown algal oligosaccharide is added to deionized water under ultrasonic condition, ultrasonically stirred and treated at 60-70℃ for 30-50 min, glycidol trimethylammonium chloride is added while stirring, and after fully stirring and mixing, the temperature is raised to 80-90℃ for reaction for 20-30 min, the temperature is then lowered to 50-60℃, deionized water and amino acid chelate are added, constant-temperature stirring is performed for 30-40 min, the temperature is then lowered to room temperature, and then glutathione and silk fibroin are added, and stirring is continued for 1-2 h.

2. A method of growing selenium enriched coriander as claimed in claim 1, wherein, In step 2), the treatment agent comprises the following components and corresponding weight percentages: fulvic acid 0.7-1.3%, lanthanum nitrate 1-2%, cerium nitrate 0.8-1.4%, phosphate ester amphoteric surfactant 7-10%, and the balance is pure water.

3. A method of growing selenium enriched coriander as claimed in claim 1, wherein, In step 2), the constant-temperature temperature is 50-70℃.

4. A method of growing selenium enriched coriander as claimed in claim 1, wherein, In step 2), the high-temperature and high-pressure treatment is performed at a temperature of 100-120℃ and a pressure of 0.2-0.5 MPa.

5. The method of growing selenium enriched coriander as claimed in claim 1 wherein, In step 3), the final concentrations of manganese sulfate, magnesium sulfate and sodium chloride are 0.02-0.03 mg / mL, 0.1-0.2 mg / mL and 0.1-0.3 mg / mL, respectively.

6. A method of growing selenium enriched coriander as claimed in claim 1, wherein, In step 3), the inoculation amount of Bacillus subtilis is 2-3%, the culture temperature is 35-39℃, and the rotation speed of the shaking bed is 120-150 rpm.

7. A method of growing selenium enriched coriander as claimed in claim 1, wherein, In 1 L of the growth regulator, the addition amounts of the raw materials are as follows: brown algal oligosaccharide 0.6-1 g, glycidol trimethylammonium chloride 1-1.5 g, amino acid chelate 0.8-1.2 g, glutathione 2-3 g, and silk fibroin 8-12 g.

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