Cutting filter screen and cell matrix separation device

Abstract

The cutting filter screen comprises a filter screen outer frame and a plurality of separators arranged in the filter screen outer frame, filter holes are formed among the separators, at least two filter holes with different and regular shapes are formed in the surface of the cutting filter screen, and a zigzag structure for cutting a cell matrix is arranged in the filter hole with at least one shape. The zigzag structure can form a cutting edge in the through hole in the filtering direction. Compared with the prior art, by means of the zigzag structures arranged in the filter holes in the cutting filter screen, when the cell matrix enters the sleeving shell and penetrates through the cutting filter screen, the cytoplasm matrix can be destroyed through the cutting edges formed by the zigzag structures, and due to the fact that the cutting edges of the cutting edges are formed in the filtering direction, the cell matrix can be conveniently cut. And the cell matrix can pass through smoothly, so that an operator can control the cell matrix to quickly penetrate through the filter screen in a reciprocating manner more conveniently, and the cutting efficiency is improved.

Description

Technical Field

[0001] The invention relates to the field of biotechnology, in particular to a cutting filter and a cell matrix separation device. Background Art

[0002] The cytoplasmic matrix refers to the liquid matrix inside the cell excluding the organelles. It is the main component of the cytoplasm and is filled with water, ions, enzymes, metabolic intermediates and a variety of solutes. In modern biomedicine, cell matrix processing is a common experimental method, which is often used to gain a deep understanding of the composition, structure and function of the cell matrix, as well as its role in health and disease.

[0003] Common cell matrix processing methods usually involve multiple steps, including but not limited to cell culture, matrix cutting, filtration, etc., and the operation is relatively cumbersome. In the prior art, some matrix cutting technologies use sharp structures on the filter to break up the cell matrix. However, since the holes on the filter are basically the same size, the filter is prone to clogging during use, and the filter needs to be frequently replaced according to the different states of cell tissues, which is relatively cumbersome. Summary of the invention

[0004] To solve the above problems, the present invention provides the following technical solutions:

[0005] A cutting filter comprises a filter outer frame and a plurality of partitions arranged inside the filter outer frame, wherein filter holes are formed between the plurality of partitions, and the filter is characterized in that at least two filter holes of different and regular shapes are arranged on its surface, and at least one filter hole of the shape is provided with a folded structure for cutting a cell matrix, wherein the folded structure can form a cutting edge inside the through hole along the filtering direction.

[0006] Based on the above technical solution, the present invention can also be improved as follows.

[0007] Furthermore, each through hole has a different cross-sectional size.

[0008] Furthermore, the lengths of intervals between edges of adjacent filter holes in all filter holes on the surface of the cut filter are consistent.

[0009] A cell matrix separation device, comprising any one of the cutting filter screens of claims 1 to 3, and also comprising a suit shell arranged outside the cutting filter screen, wherein the surface of the suit shell is provided with a liquid inlet and a liquid outlet, wherein the cutting filter screen is arranged at a position between the liquid inlet and the liquid outlet inside the suit shell, and the cutting filter screen can separate the inside of the suit shell into two chambers

[0010] Furthermore, the suit shell includes an upper shell and a lower shell matched with the upper shell, and the liquid inlet and the liquid outlet are respectively distributed on the surface of the upper shell and the lower shell.

[0011] Beneficial Effects

[0012] 1. Compared with the prior art, the present invention uses the folded structure provided in the filter hole on the cutting filter, so that when the cell matrix enters the suit shell and passes through the cutting filter, the cytoplasmic matrix can be destroyed by the cutting blade formed by the folded structure. Since the cutting edge of the cutting blade is formed along the filtering direction, the cell matrix can pass through more smoothly, which is convenient for the operator to control the cell matrix to quickly reciprocate through the filter, thereby improving the cutting efficiency.

[0013] 2. Compared with the prior art, the present invention uses filter holes of different sizes and regular shapes. When the filter holes with smaller pore sizes are clogged, the filter holes with larger pore sizes can also ensure the smooth flow of the filter screen, thereby effectively reducing the clogging situation. Furthermore, the folded structure formed by the partition and the spacing between the edges of each filter hole can more effectively utilize the entire space on the filter screen, further increase the number of filter holes, improve cutting efficiency, and further reduce clogging situations. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0015] Figure 1 To invent a schematic diagram of an explosion stereoscopic structure;

[0016] Figure 2 A schematic diagram of the three-dimensional structure of the invented cutting filter;

[0017] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0018] 1. Filter frame; 2. Partition; 3. Folding structure; 4. Suit shell; 41. Upper shell; 42. Lower shell. DETAILED DESCRIPTION

[0019] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the relevant drawings. Several embodiments of the present invention are given in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.

[0020] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0021] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0022] It should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside", etc. indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings, or are directions or positional relationships in which the technical products are usually placed when in use. They are only for the convenience of describing the present technology and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as a limitation on the present technology.

[0023] See also Figure 1-2 The cutting filter of the present invention comprises a filter outer frame 1, and a plurality of partitions 2 arranged inside the filter outer frame 1, and filter holes are formed between the plurality of partitions 2, wherein the filter outer frame 1 and the partitions 2 can both be made of common metal materials used for biological experiments in the prior art, and can be directly stamped by equipment or processed by metal parts. Different from the prior art, in order to ensure the passability of the cutting filter, at least two filter holes of different and regular shapes are provided on the surface of the cutting filter, and at least one filter hole of a certain shape is provided with a folding structure 3 for cutting the cell matrix, and the folding structure 3 can form a cutting edge inside the through hole along the filtering direction, and the folding structure 3 can be a right-angle structure as shown in the figure, or it can be an obtuse-angle structure, aiming to form one or more edges at its bending point, and when the cell matrix is ​​subjected to pressure to pass through the filter hole, a part of it can hit the edge and be cut by the edge.

[0024] The edge of the cutting blade may be sharp or blunt, and the entire blade may be slightly inclined to increase the contact area with the cell matrix during the filtration process.

[0025] To explain the working principle and function of the cutting filter in detail, a cell matrix separation device using a cutting filter is disclosed, which also includes a set shell 4 arranged outside the cutting filter, and a liquid inlet and a liquid outlet are provided on the surface of the set shell 4. The cutting filter is arranged at a position between the liquid inlet and the liquid outlet inside the set shell 4, and the cutting filter can separate the inside of the set shell 4 into two independent chambers, wherein a fixed structure and a sealing structure compatible with the cutting filter can be arranged inside the set shell 4 to ensure the stability of its use process. After the cell matrix is ​​injected into the set shell 4, the cell tissue is cut and filtered by the cutting filter, and then removed from the set shell 4. Specifically, the set shell 4 includes an upper shell 41 and a lower shell 42 compatible with the upper shell 41, and the liquid inlet and the liquid outlet are respectively distributed on the surfaces of the upper shell 41 and the lower shell 42. The user can use an external device, such as a syringe, to achieve repeated cutting of the cell tissue on the cutting filter.

[0026] Embodiment 1:

[0027] On the basis of the above technical solution, in order to reduce the clogging of the cutting filter, the cross-sectional sizes of each through hole provided on the surface of the cutting filter are different. Preferably, two filter holes of different sizes are provided on the surface of the cutting filter. When the filter hole with a smaller pore size is clogged, the filter hole with a larger pore size can ensure that the normal filtration of the filter is not greatly affected.

[0028] Embodiment 2:

[0029] Different from Example 1, on the basis of Example 1, in order to ensure the uniform distribution of filter holes, the interval lengths between the edges of adjacent filter holes in all the filter holes on the surface of the cut filter are consistent, the shapes of the filter holes can be consistent, but the sizes are inconsistent. By limiting the spacing and aperture of the filter holes, it is ensured that the surface of the filter can be fully utilized, thereby achieving uniform and dense distribution of the filter holes.

[0030] Embodiment 3:

[0031] What is different from Example 2 is that, based on Example 1 and Example 2, referring to the accompanying drawings, there are two kinds of filter holes on the cutting filter, one of which is a cross-shaped structure, and the other is a shape structure formed by overlapping a cross and a rectangle. The bending angle of the folded structure 3 provided inside the two is greater than or equal to ninety degrees. In this embodiment, the two kinds of filter holes are staggered and evenly distributed on the cutting filter, filling up the entire space on the cutting filter, effectively increasing the density of the filter holes on the cutting filter, and coordinating with filter holes of different sizes and the cutting blades inside the filter holes, further enhancing the permeability and cutting efficiency of cell tissue.

[0032] In the description of this technology, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this technology can be understood according to specific circumstances.

[0033] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A cutting filter, comprising a filter frame (1), and a plurality of partitions (2) arranged inside the filter frame (1), wherein filter holes are formed between the plurality of partitions (2), characterized in that: The surface is provided with at least two filter holes of different and regular shapes, and a folded structure (3) for cutting the cell matrix is ​​provided in at least one filter hole of the shape, and the folded structure (3) can form a cutting edge inside the through hole along the filtering direction.

2. A cutting filter according to claim 1, characterized in that: The cross-sectional sizes of each through hole arranged on the surface of the cutting filter are different.

3. A cutting filter according to claim 2, characterized in that: The interval lengths between the edges of adjacent filter holes in all the filter holes on the surface of the cut filter screen are consistent.

4. A cell matrix separation device, comprising any one of the cutting filters described in claims 1 to 3, and also comprising a casing (4) arranged outside the cutting filter, wherein a liquid inlet and a liquid outlet are arranged on the surface of the casing (4), and the cutting filter is arranged at a position between the liquid inlet and the liquid outlet inside the casing (4), and the cutting filter can separate the inside of the casing (4) into two chambers.

5. A cell matrix separation device according to claim 4, characterized in that: The suit shell (4) comprises an upper shell (41) and a lower shell (42) matched with the upper shell (41), and the liquid inlet and the liquid outlet are respectively distributed on the surfaces of the upper shell (41) and the lower shell (42).

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