Optical sensor and photosynthetic organism growth monitoring method

Abstract

The application provides an optical sensor for monitoring growth information of photosynthetic organisms, which comprises a main body formed with a transmitting window and a receiving window arranged oppositely and at intervals; a light source located in the main body for transmitting first reference light and second reference light with different wavelengths through the transmitting window; a receiver located in the main body for receiving first detection light generated after the first reference light passes through the photosynthetic organisms through the receiving window, and for receiving second detection light generated after the second reference light passes through the photosynthetic organisms through the receiving window, and for generating an electrical signal according to the first detection light and the second detection light; and a control circuit electrically connected with the light source and the receiver for receiving the electrical signal and obtaining the growth information of the photosynthetic organisms based on the electrical signal. The application also provides a photosynthetic organism growth monitoring method.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of photosynthetic organism monitoring culture, and in particular to an optical sensor and a photosynthetic organism growth monitoring method applied to the optical sensor. BACKGROUND

[0002] The pigment content and composition in the cells of photosynthetic organisms are quite different in different growth periods, and it is difficult to calculate the dry weight by using ordinary optical sensors to measure the absorbance. SUMMARY

[0003] The first aspect of the present application provides an optical sensor for monitoring growth information of photosynthetic organisms, the optical sensor comprising: a main body, which is formed with a transmitting window and a receiving window arranged oppositely and spaced apart; a light source located in the main body, configured to emit first reference light and second reference light with different wavelengths through the transmitting window; a receiver located in the main body, configured to receive first detection light generated after the first reference light passes through the photosynthetic organisms through the receiving window, and configured to receive second detection light generated after the second reference light passes through the photosynthetic organisms through the receiving window, and configured to generate an electrical signal according to the first detection light and the second detection light; and a control circuit electrically connected to the light source and the receiver, configured to receive the electrical signal, and configured to obtain the growth information of the photosynthetic organisms based on the electrical signal.

[0004] The second aspect of the present application provides a photosynthetic organism growth monitoring method applied to the optical sensor, the photosynthetic organism growth monitoring method comprising: controlling the light source to emit first reference light and second reference light; controlling the receiver to receive first detection light and second detection light through the receiving window, and receiving an electrical signal from the receiver, the electrical signal being generated according to the first detection light and the second detection light; and obtaining the growth information of the photosynthetic organisms based on a preset absorption spectrum curve according to the electrical signal.

[0005] The optical sensor and the photosynthetic organism growth monitoring method described above, by emitting first reference light and second reference light with different wavelengths by the light source, detect the growth information of the photosynthetic organisms based on the reference light with different wavelengths. Since the reference light with different wavelengths has different optical characteristics, the different growth information of the photosynthetic organisms (such as microalgae, microfern, etc.) detected by the reference light with different wavelengths is comprehensive, and the detection data is more accurate. The optical sensor can be applied to the photosynthetic organism culture system to continuously monitor the growth of the photosynthetic organisms for a long time, and is of great significance especially for the photosynthetic organism culture system of constant turbidity continuous culture. BRIEF DESCRIPTION OF DRAWINGS

[0006] Figure 1 FIG. 1 is a perspective view of the optical sensor according to an embodiment of the present application. FIG. 2 is a schematic view of the optical sensor according to an embodiment of the present application.

[0007] Figure 2 Another perspective view of the optical sensor according to an embodiment of the present application.

[0008] Figure 3 An exploded view of the optical sensor according to an embodiment of the present application.

[0009] Figure 4 A perspective view of the optical sensor according to an embodiment of the present application in a detection state.

[0010] Figure 5 A flowchart of steps of the photosynthetic organism growth monitoring method according to an embodiment of the present application.

[0011] Explanation of main element symbols

[0012] Optical sensor: 1;

[0013] Main body: 10;

[0014] First mounting portion: 11;

[0015] First surface: 111;

[0016] Emission window: 112;

[0017] Second mounting portion: 12;

[0018] Second surface: 121;

[0019] Reception window: 122;

[0020] Connecting column: 13;

[0021] Accommodation space: 14;

[0022] Light source: 20;

[0023] Receiver: 30;

[0024] Rotor assembly: 40;

[0025] Driving rotor: 41;

[0026] Light shielding portion: 411;

[0027] Light transmitting portion: 412;

[0028] Driven rotor: 42;

[0029] Bearing: 43;

[0030] Motor: 50;

[0031] Signal line: 60;

[0032] Rotating shaft: L.

[0033] The following detailed description will further explain the present application with reference to the above mentioned drawings. DETAILED DESCRIPTION

[0034] The present application provides an optical sensor applied in a photosynthetic organism culture system, which can monitor the growth state of photosynthetic organisms (such as microalgae, micro-fern, etc.) in real time and continuously return photosynthetic organism growth information (including the cell dry weight, pigment content, cell wall component content, cell membrane component content, etc. of photosynthetic organisms). In the present embodiment, the structure and function of the optical sensor are exemplified by taking the optical sensor for detecting the growth information of microalgae cells as an example.

[0035] Referring to Figure 1 , the optical sensor 1 of the present embodiment comprises a main body 10, a light source 20 and a receiver 30 located in the main body 10. The light source 20 is used to emit reference light, and the reference light is received by the receiver 30 as detection light after passing through the algal culture liquid. The receiver 30 is used to generate an electrical signal based on the detection light. The electrical signal can be used to obtain the growth information of microalgae cells in the culture medium.

[0036] In the present embodiment, the main body 10 has a first mounting portion 11, a second mounting portion 12 and four connecting columns 13. The first mounting portion 11 and the second mounting portion 12 are both substantially hollow cuboid structures. The connecting columns 13 are parallel to each other and spaced apart. Each connecting column 13 extends from one corner of the first mounting portion 11 to one corner of the second mounting portion 12. In the present embodiment, the first mounting portion 11, the second mounting portion 12 and the connecting columns 13 are integrally formed.

[0037] Referring to Figure 1 and Figure 2 , the first mounting portion 11 has a first surface 111 facing the second mounting portion 12, and the first surface 111 is formed with an emission window 112. The light source 20 is located in the first mounting portion 11 and at the emission window 112, and is used to emit reference light through the emission window 112.

[0038] The second mounting portion 12 has a second surface 121 facing the first mounting portion 11, and the second surface 121 is formed with a receiving window 122. The first surface 111 and the second surface 121 are parallel to each other and spaced apart. The receiver 30 is located in the second mounting portion 12 and at the receiving window 122, and is used to receive detection light incident from the receiving window 122.

[0039] The regions of the main body 10 where the emission window 112 and the receiving window 122 are formed are made of light-transmitting material, and the remaining regions are made of light-blocking material. Since the optical sensor 1 is always immersed in the algal culture liquid during use, the main body 10 is made of PC plastic, which can resist corrosion, dirt and other forms of damage caused by exposure to salt water or other substances in water.

[0040] In this embodiment, the emission window 112 and the receiving window 122 are circular windows. The emission window 112 and the receiving window 122 have the same area, or the receiving window 122 has a slightly larger area than the emission window 112, to facilitate more efficient reception of the detection light. The orthographic projection of the receiving window 122 on the first surface 111 completely covers the emission window 112, which on the one hand facilitates more efficient reception of the detection light, and on the other hand enables the reference light emitted from the emission window 112 to enter the receiving window 122 as a horizontal detection light, which facilitates maximum reduction of light attenuation and distortion caused by reflection, refraction, and scattering during light propagation.

[0041] In this embodiment, the four connecting columns 13 form a receiving space 14 between the first mounting portion 11 and the second mounting portion 12. When the optical sensor 1 is immersed in the microalgae culture solution, the culture algae solution can enter the receiving space 14 from the area between the connecting columns 13. At this time, the reference light emitted from the emission window 112 passes through the culture algae solution, causing the optical properties of the reference light to change, and the reference light enters the receiving window 122 as detection light.

[0042] The light source 20 is used to emit first reference light and second reference light with different wavelengths simultaneously or at different times. In this embodiment, the first reference light is infrared light with a wavelength of 940 nm, and the second reference light is light with a wavelength of 450 nm. The optical sensor 1 uses the light source 20 with the above-mentioned specific wavelengths to accurately measure the concentration of microalgae cells by using the absorption characteristics of microalgae cells to different wavelengths of light.

[0043] The first reference light with a wavelength of 940 nm (infrared light) has the following characteristics:

[0044] 1. Water absorption characteristics: Light with a wavelength of 940 nm has less absorption in water, so it can penetrate the water layer without losing too much energy. This is particularly important for measuring the concentration of microalgae in water, as it reduces the interference of water with the light signal.

[0045] 2. Cell structure penetration: Infrared light can penetrate cell walls and cell membranes to reach the interior of the cell. This enables the measurement of the properties of the entire cell, not just the cell surface.

[0046] 3. Less absorption by chlorophyll: Compared to light of other wavelengths, chlorophyll has less absorption of light with a wavelength of 940 nm, so it can reduce the interference of chlorophyll with the measurement signal, making the measurement result more accurate.

[0047] The second reference light with a wavelength of 450 nm has the following characteristics:

[0048] 1. Chlorophyll absorption peak: The light at 450 nm wavelength is located in a peak region of the chlorophyll absorption spectrum. Chlorophyll is an important pigment for microalgae to perform photosynthesis, which strongly absorbs light in the blue region, especially around 450 nm.

[0049] 2. Measure photosynthetic activity: By measuring the absorption of 450 nm light by microalgae, the activity of photosynthesis and biomass can be indirectly evaluated. This is because the amount of light absorbed by chlorophyll (chlorophyll content) is directly related to the growth status of the cell (under suitable light intensity, cells in good growth status usually have higher chlorophyll content. Under suitable light conditions, the growth rate of the cell usually increases, which means that the cell needs more energy and reducing power (such as ATP and NADPH) to support biosynthesis. Therefore, the cell will increase the content of chlorophyll to enhance photosynthesis to meet these needs. With the aging of the cell, the content of chlorophyll usually decreases, because the degradation rate of chlorophyll molecules exceeds the synthesis rate. The decrease of chlorophyll content will lead to the decrease of photosynthetic efficiency, the decrease of cell growth and metabolic activity, and eventually may lead to cell death).

[0050] Therefore, in this embodiment, the first reference light at 940 nm and the second reference light at 450 nm are selected, so that the optical sensor 1 can combine the measurement data of the two, compare and analyze the light absorption at different wavelengths, more accurately calculate the concentration of microalgae and evaluate its growth status, which is beneficial to improve the measurement accuracy, reduce errors, and can provide important information about the growth status of microalgae.

[0051] In this embodiment, the light source 20 includes two light-emitting diodes that can emit the first reference light and the second reference light, respectively. In other embodiments of the present application, the wavelength of the second reference light can be changed according to the different information to be detected.

[0052] The receiver 30 is used to receive the first detection light generated after the first reference light passes through the cultured algal liquid through the receiving window 122, and is used to receive the second detection light generated after the second reference light passes through the cultured algal liquid through the receiving window 122. In this embodiment, the receiver 30 includes a photodiode, which is used to convert the first detection light and the second detection light into an electrical signal output through photoelectric conversion.

[0053] In this embodiment, the optical sensor 1 further includes a rotor assembly 40 movably connected between the first mounting portion 11 and the second mounting portion 12. That is, the rotor assembly 40 is movably connected between the first surface 111 and the second surface 121, and is located in the accommodation space 14.

[0054] Please refer to Figure 3The rotor assembly 40 comprises a driving rotor 41, a driven rotor 42 and a bearing 43. The driving rotor 41 and the driven rotor 42 are separate structures and are arranged around the bearing 43. The optical sensor 1 further comprises a motor 50. The motor 50 is fixedly arranged on the side of the first mounting portion 11 away from the first surface 111 and is connected with the driving rotor 41 for driving the driving rotor 41 to rotate around the rotation axis L (i.e. around the axis of the bearing 43). The motor 50 can drive the driving rotor 41 to rotate forward (clockwise around the rotation axis L) and reverse (counterclockwise around the rotation axis L). The rotation axis L is perpendicular to the first surface 111 and the second surface 121 respectively.

[0055] The driven rotor 42 is not connected with the motor 50. When the driven rotor 42 is located in the rotation direction of the driving rotor 41, the driving rotor 41 pushes the driven rotor 42 to rotate synchronously after contacting the driven rotor 42.

[0056] In the embodiment, the driving rotor 41 and the driven rotor 42 are both solid columnar structures. The two ends of the driving rotor 41 and the driven rotor 42 respectively contact the first surface 111 and the second surface 121. During the rotation around the rotation axis L, the two ends of the driving rotor 41 and the driven rotor 42 always respectively contact the first surface 111 and the second surface 121.

[0057] In the embodiment, the two ends of the driving rotor 41 and the driven rotor 42 contacting the first surface 111 jointly cover part of the first surface 111, so that the emission window 112 can be exposed relative to the rotor assembly 40, avoiding that the driving rotor 41 and / or the driven rotor 42 cover the emission window 112 to affect the emission of the reference light. Similarly, the two ends of the driving rotor 41 and the driven rotor 42 contacting the second surface 121 jointly cover part of the second surface 121, so that the receiving window 122 can be exposed relative to the rotor assembly 40, avoiding that the driving rotor 41 and / or the driven rotor 42 cover the receiving window 122 to affect the reception of the detection light.

[0058] The driving rotor 41 comprises a light-shielding portion 411 and a light-transmitting portion 412. The light-transmitting portion 412 is embedded in the light-shielding portion 411. The light-shielding portion 411 has two ends respectively contacting the first surface 111 and the second surface 121, and a through hole is formed in the light-shielding portion 411. The light-transmitting portion 412 is filled in the through hole and is exposed relative to the two ends of the light-shielding portion 411. The two ends of the light-transmitting portion 412 also respectively contact the first surface 111 and the second surface 121. The end of the light-transmitting portion 412 contacting the first surface 111 has the same shape and area as the emission window 112, and the end of the light-transmitting portion 412 contacting the second surface 121 has the same shape and area as the receiving window 122.

[0059] In this embodiment, the light shielding part 411 and the passive rotor 42 are made of PC plastic, which can resist corrosion, dirt and other forms of damage caused by exposure to salt water or other substances in water, and the light transmitting part 412 is a cylindrical glass component.

[0060] In this embodiment, the optical sensor 1 realizes the functions of monitoring the growth state of microalgae, and also realizes the functions of light shielding, self-calibration and self-cleaning of the optical sensor 1 through the rotor assembly 40. In the process of realizing the above functions, the active rotor 41 and the passive rotor 42 may need to rotate to shield part or all of the areas of the emission window 112 and the receiving window 122. In order to ensure that the active rotor 41 and the passive rotor 42 can shield part or all of the areas of the emission window 112 and the receiving window 122, in this embodiment, the emission window 112 and the receiving window 122 are located in the rotation path range of the active rotor 41 and the passive rotor 42. In this embodiment, the rotation path range of the active rotor 41 and the passive rotor 42 is defined as the range of the first surface 111 and the second surface 121 passed by the active rotor 41 and the passive rotor 42 during 360 rotation around the rotation axis L.

[0061] In this embodiment, the optical sensor 1 further comprises a signal line 60 fixedly connected to the main body 10 and a control circuit (not shown in the figure) located in the main body 10. The control circuit is electrically connected with the light source 20, the receiver 30, the motor 50 and the signal line 60 (for example, the electrical connection between the elements is realized by arranging wires in the main body 10) for controlling the light source 20 to emit the first reference light and the second reference light, controlling the motor 50 to drive the active rotor 41 to rotate, receiving the electrical signal from the receiver 30, and generating the microalgae growth information according to the electrical signal. The signal line 60 is also connected to the equipment outside the optical sensor 1 for transmitting the growth information obtained by the control circuit to the external equipment for display, recording or further analysis.

[0062] The working mode of the optical sensor 1 is described as follows, it should be noted that the optical sensor 1 remains immersed in the microalgae culture solution during the working process.

[0063] I. Self-calibration

[0064] Since there may be degree differences between different optical sensors 1, the optical sensor 1 is calibrated before it is put into formal use.

[0065] The control circuit controls the motor 50 to work, and drives the main rotor 41 to rotate through the motor 50, until the light-transmitting part 412 in the main rotor 41 rotates to the optical window, and completely covers the optical window (that is, the end of the light-transmitting part 412 contacting the first surface 111 completely covers the emitting window 112, and the end of the light-transmitting part 412 contacting the second surface 121 completely covers the receiving window 122). Since the light-transmitting part 412 has high light-transmitting property, its transmittance is 1%-100%, at this time, the reference light emitted by the emitting window 112 can directly propagate to the receiving window 122 along the light-transmitting part 412. At this time, the detection light received by the receiver 30 has the maximum light intensity, and the amplitude of the electrical signal generated at this time is recorded.

[0066] When the light-blocking part 411 completely covers the optical window, the reference light emitted by the emitting window 112 is completely blocked by the light-blocking part 411, and the receiver 30 can hardly receive the detection light, that is, at this time, the detection light received by the receiver 30 has the minimum light intensity, and the amplitude of the electrical signal generated at this time is recorded.

[0067] According to the amplitudes of the electrical signals recorded at the times of the maximum and minimum light intensities, the correspondence between the detection light intensity and the electrical signal amplitude of the optical sensor 1 currently used can be obtained, that is, the calibration of the optical sensor 1 is completed, which is beneficial to improve the detection precision.

[0068] II. Light blocking

[0069] The optical sensor 1 works in an open state (as shown in FIG. 2) and a detection state (as shown in FIG. 3) in time. Figures 1-2 Figure 4

[0070] When the optical sensor 1 works in the open state, the growth state of the microalgae is not detected, and the light source 20 does not emit the reference light. At this time, the main rotor 41 and the passive rotor 42 are close to each other, and a large open space is formed between the emitting window 112 and the receiving window 122, so that the culture algal liquid (including microalgae cells, water, culture medium, etc.) can be conveniently put into the open space.

[0071] ​​At a certain moment, the optical sensor 1 needs to be switched from the open state to the detection state. At this time, the control circuit controls the motor 50 to drive the driving rotor 41 to rotate towards the side where the driven rotor 42 is located, so that the driving rotor 41 pushes the driven rotor 42 to rotate synchronously until the driven rotor 42 is pushed to a preset position (any position that does not cover the optical window) and then stopped, and then the motor 50 drives the driving rotor 41 to rotate in the opposite direction by a certain angle and is spaced from the driven rotor 42. In this way, the open space between the emission window 112 and the receiving window 122 is reduced, and at this time the culture algal liquid in the open space is not easy to flow with the external culture algal liquid compared with the open state. And at this time the driven rotor 42 can also block external light (light other than the reference light emitted by the light source 20) to a certain extent, reducing noise interference.

[0072] III. Detecting growth state:

[0073] During the detection of microalgae cell growth information, the driving rotor 41 and the driven rotor 42 remain stationary, and at this time the emission window 112 and the receiving window 122 are completely exposed to the driving rotor 41 and the driven rotor 42. The control circuit transmits a control signal to the light source 20 to drive the light source 20 to emit the first reference light and / or the second reference light simultaneously or at different times. After the first reference light and / or the second reference light passes through the culture algal liquid (including microalgae cells, water, culture medium, etc.), it is output as the first detection light and / or the second detection light level to the receiving window 122. The receiver 30 receives the first detection light and / or the second detection light from the receiving window 122, performs photoelectric conversion and outputs corresponding electrical signals. The control circuit calculates the corresponding growth data (including the cell dry weight, pigment content, cell wall component content, cell membrane component content, etc. of the microalgae cells) according to the electrical signals and transmits them to the external device through the signal line 60.

[0074] IV. Self-cleaning of optical window (emission window 112 and receiving window 122):

[0075] Since the optical sensor 1 is continuously immersed in the microalgae culture solution during operation, as the microalgae cells grow, the microalgae cells, biofilm, sediments and other particles can be attached and accumulated on the first surface 111, the second surface 121 and the accommodation space 14, so that the emission window 112 and the receiving window 122 are blocked, or the light propagation path is distorted, thereby affecting the detection accuracy. Therefore, in the embodiment, the motor 50 drives the driving rotor 41 to rotate around the rotation axis L. Since the two ends of the driving rotor 41 respectively contact the first surface 111 and the second surface 121, when the driving rotor 41 rotates around the rotation axis, the driving rotor 41 rubs against the first surface 111 and the second surface 121, which can remove the microalgae cells, biofilm, sediments and other particles attached to the first surface 111 and the second surface 121, and also clean the microalgae cells, biofilm, sediments and other particles in the accommodation space 14, thereby cleaning the optical window and the accommodation space 14, which is conducive to ensuring that the optical sensor 1 can provide accurate and reliable data for a long time even in harsh underwater environments.

[0076] The optical sensor 1 as described above in the present application emits first reference light and second reference light with different wavelengths through the light source 20 to detect the growth information of photosynthetic organisms based on reference light with different wavelengths. Since the reference light with different wavelengths has different optical characteristics, the different growth information of photosynthetic organisms detected by different wavelengths of reference light is integrated, and the detection data is more accurate. In the embodiment, the first reference light is infrared light with a wavelength of 940 nm, and the second reference light is light with a wavelength of 450 nm. The above two wavelengths of reference light can enable the optical sensor to detect the content of components such as pigments, cell walls or cell membranes. The growth state of cells (chlorophyll content) and cell concentration can be calculated according to the pigments, and the cell density can be calculated according to the cell structure components.

[0077] In addition, in the embodiment, the orthographic projection of the receiving window 122 on the first surface 111 completely covers the emission window 112, that is, the emission window 112 and the receiving window 122 are arranged opposite to each other. The first reference light and the second reference light emitted from the emission window 112 can be horizontally reflected to the receiving window as first detection light and second detection light after passing through the light and the organism, which is conducive to simplifying the optical path and reducing light loss.

[0078] Further, the optical sensor 1 in the embodiment further comprises a rotor assembly 40, the rotor assembly 40 comprises a driving rotor 41 and a driven rotor 42 arranged separately, by driving the driving rotor 41 and the driven rotor 42 to rotate, and by setting the movement process of the driving rotor 41 and the driven rotor 42, the optical sensor 1 can realize self-calibration, light shielding and self-cleaning functions when immersed in the algal liquid. In this way, the optical sensor 1 can be applied to the photosynthetic organism culture system to monitor the growth of the photosynthetic organism for a long time, which is of great significance especially for the photosynthetic organism culture system of constant turbidity continuous culture. By connecting an external device through a signal line, the optical sensor 1 can also be remotely controlled and the growth information of the photosynthetic organism can be remotely obtained.

[0079] The embodiment of the present application also provides a photosynthetic organism growth monitoring method applied to the control circuit of the optical sensor 1. Please refer to Figure 5 The photosynthetic organism growth monitoring method comprises the following steps:

[0080] Step S1, determining an absorption spectrum curve according to the type of photosynthetic organism;

[0081] Step S2, controlling the motor to drive the driving rotor to rotate, so that the light transmission part of the driving rotor completely covers the emission window and the receiving window, to calibrate the optical sensor;

[0082] Step S3, after controlling the motor to drive the driving rotor to drive the driven rotor to rotate synchronously, controlling the motor to drive the driving rotor to rotate in the opposite direction, to switch the optical sensor to a detection state;

[0083] Step S4, controlling the light source to emit first reference light and second reference light;

[0084] Step S5, controlling the receiver to receive first detection light and second detection light through the receiving window, and receiving an electrical signal from the receiver, the electrical signal being generated according to the first detection light and the second detection light;

[0085] Step S6, removing noise in the electrical signal;

[0086] Step S7, based on the absorption spectrum curve, obtaining growth information of the photosynthetic organism according to the electrical signal.

[0087] In the embodiment, after step S6, the following steps are further included:

[0088] Step S8, judging whether it is the cleaning time.

[0089] If the answer of step S8 is yes, step S9 is executed: controlling the motor to drive the driving rotor to rotate in two directions to perform self-cleaning.

[0090] If the step S8 is determined as no, then the step S10 is performed: the motor is controlled to drive the driving rotor to rotate the driven rotor until the optical sensor is switched to the open state.

[0091] After the optical sensor is switched to the open state, the optical sensor can be switched to the detection state again for detection after a preset time interval, that is, the step S3 is performed again.

[0092] It should be understood that the above step numbers are not used to limit the order of performing the steps.

[0093] The photosynthetic organism growth monitoring method based on the optical sensor 1 as described above can achieve all the beneficial effects of the optical sensor 1 as described above. On this basis, the photosynthetic organism growth monitoring method is particularly suitable for the photosynthetic organism culture system of constant turbidity continuous culture, and continuously monitors and outputs the growth data of the photosynthetic organism in the photosynthetic organism culture process.

[0094] Those skilled in the art should understand that the above embodiments are only used to illustrate the present application, and are not used as a limitation on the present application, and as long as the above embodiments are within the spirit and scope of the present application, any suitable changes and variations of the above embodiments are within the scope of the present application.

Claims

1. An optical sensor, characterized in that, Used to monitor the growth information of photosynthetic organisms; The optical sensor includes: The main body consists of a transmission window and a reception window that are positioned opposite each other and spaced apart. A light source, located within the main body, is used to emit first and second reference lights of different wavelengths through the emission window; A receiver, located within the main body, is used to receive, through the receiving window, the first detection light generated by the first reference light after passing through the photosynthetic organism, and to receive, through the receiving window, the second detection light generated by the second reference light after passing through the photosynthetic organism, and to generate an electrical signal based on the first detection light and the second detection light. Electric motor; A rotor assembly, movably connected to the main body, includes a separate active rotor and a passive rotor. The active rotor is connected to the motor, and the passive rotor can rotate synchronously with the active rotor under its drive. The control circuit is electrically connected to the light source, the receiver, and the motor, and is used to control the motor to drive the active rotor to rotate, receive the electrical signal, and obtain the growth information of the photosynthetic organism based on the electrical signal.

2. The optical sensor as described in claim 1, characterized in that, The first reference light is infrared light, and the second reference light has a wavelength of 450nm.

3. The optical sensor as described in claim 2, characterized in that, The wavelength of the first reference light is 940 nm.

4. The optical sensor as described in claim 1, characterized in that, The rotor assembly is located between the transmitting window and the receiving window; The rotor assembly is rotatable about a rotation axis that is perpendicular to the transmitting window and the receiving window.

5. The optical sensor as described in claim 4, characterized in that, The motor is used to drive the active rotor to rotate around the rotation axis.

6. The optical sensor as described in claim 5, characterized in that, The main body has a first surface and a second surface that are parallel to each other, the transmitting window is formed on the first surface, the receiving window is formed on the second surface, and the rotation axis is perpendicular to the first surface and the second surface; The two ends of the active rotor and the passive rotor are in contact with the first surface and the second surface, respectively.

7. The optical sensor as described in claim 6, characterized in that, The active rotor includes a light-shielding part and a light-transmitting part. The light-transmitting part is embedded in the light-shielding part, and the two ends of the light-transmitting part that contact the first surface and the second surface are exposed relative to the light-shielding part. The two ends of the light-transmitting part contact the first surface and the second surface, respectively.

8. The optical sensor as described in claim 6, characterized in that, The orthographic projection of the receiving window onto the first surface completely covers the transmitting window.

9. The optical sensor as described in claim 6, characterized in that, The passive rotor is made of light-shielding material.

10. The optical sensor as described in any one of claims 1-9, characterized in that, The growth information includes one or any combination of the photosynthetic organism's cell dry weight, pigment content, cell wall component content, and cell membrane component content.

11. The optical sensor as described in any one of claims 1-9, characterized in that, It also includes signal lines that are fixedly mounted on the main body and electrically connected to the control circuit.

12. A method for monitoring the growth of photosynthetic organisms, characterized in that, Applied to the optical sensor as described in claim 1; The method for monitoring the growth of photosynthetic organisms includes: Control the light source to emit a first reference light and a second reference light; The control receiver receives a first detection light and a second detection light through the receiving window, and receives an electrical signal from the receiver, the electrical signal being generated based on the first detection light and the second detection light; Based on a preset absorption spectrum curve, the growth information of the photosynthetic organism is obtained according to the electrical signal; The control motor drives the active rotor to rotate, so that the passive rotor rotates synchronously with the active rotor under the push of the active rotor.

13. The method for monitoring the growth of photosynthetic organisms as described in claim 12, characterized in that, The rotor assembly is fixedly mounted on the motor of the main body and located between the transmitting window and the receiving window; Following the step of receiving an electrical signal from the receiver, the photosynthetic organism growth monitoring method further includes: The control motor drives the rotor assembly to rotate to perform self-cleaning; The step of controlling the motor to drive the active rotor to rotate includes: The control motor drives the rotor assembly to rotate in order to switch the optical sensor to the open state.

14. The method for monitoring the growth of photosynthetic organisms as described in claim 13, characterized in that, The rotor assembly includes an active rotor, and the active rotor includes a light-transmitting portion; The photosynthetic organism growth monitoring method further includes, prior to the step of controlling the light source to emit the first reference light and the second reference light: The motor is controlled to drive the active rotor to rotate, so that the light-transmitting part completely covers the transmitting window and the receiving window, in order to calibrate the optical sensor.

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