A method for cutting propagation of a mongolian medicine plant artemisia dubia clandon

Abstract

The application belongs to the technical field of plant cultivation, and particularly relates to a cutting propagation method of Mongolian medicine plant artemisia dubia, which comprises the following steps: S1, disinfecting artemisia dubia branches; S2, pruning the artemisia dubia branches after disinfection, and obtaining artemisia dubia cutting segments after re-disinfection; S3, treating the artemisia dubia cutting segments with a rooting agent, and cutting the artemisia dubia cutting segments in a substrate for cutting propagation. The cutting propagation method of the Mongolian medicine plant artemisia dubia exhibits significant beneficial effects in protecting ecological environment, improving propagation efficiency, enriching germplasm resources, enhancing stress resistance, and improving economic benefits, and has important popularization and application value.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of plant cultivation, and particularly relates to a cutting propagation method of a Mongolian medicine plant Artemisia caruifolia. BACKGROUND

[0002] Artemisia caruifolia is the aerial part of Artemisia caruifolia of the family Asteraceae, which is warm in nature and pungent in taste, has the effects of moistening dryness and removing dampness, relieving cough and pain, clearing heat and promoting diuresis, and has the effects of relieving inflammation and killing insects, and is a commonly used dry Xiriyusu characteristic Mongolian medicine. At present, wild resources picking is mainly used in the production of Artemisia caruifolia, and seed breeding is carried out in some areas. Since Artemisia caruifolia belongs to water body maintaining plants, the growth mode of Artemisia caruifolia is closely related to the growth environment, and wild picking of Artemisia caruifolia can easily destroy the environment, cause soil erosion, increase the degree of grassland desertification, and the seed breeding of Artemisia caruifolia has low seedling emergence rate, scarce germplasm resources, weak resistance degree at the seedling stage, and too slow growth cycle. Therefore, it is necessary to develop a cutting propagation method of Artemisia caruifolia. SUMMARY

[0003] The purpose of the present application is to provide a cutting propagation method of a Mongolian medicine plant Artemisia caruifolia, so as to overcome the shortcomings of the prior art, adopt cutting propagation, have simple operation steps, short seedling period, large number of seedlings per unit area, short seedling period after planting, high survival rate, improve the propagation efficiency, and be easy to popularize and apply.

[0004] In order to achieve the above-mentioned purpose, the technical scheme of the present application is as follows:

[0005] The present application provides a cutting propagation method of a Mongolian medicine plant Artemisia caruifolia, comprising the following steps:

[0006] S1, disinfecting Artemisia caruifolia branches;

[0007] S2, pruning the disinfected Artemisia caruifolia branches, and obtaining Artemisia caruifolia cutting segments after re-disinfection;

[0008] S3, treating the Artemisia caruifolia cutting segments with a rooting agent, and cutting the Artemisia caruifolia cutting segments in the substrate for cutting propagation.

[0009] In some other embodiments, in step S1, the Artemisia caruifolia branches are two to three-year-old Artemisia caruifolia branches with a growth time of more than 60 days; preferably, the Artemisia caruifolia branches are two-year-old Artemisia caruifolia branches with a growth time of more than 60 days.

[0010] In some other embodiments, in step S1, the Artemisia caruifolia branches are picked between 9:00 and 11:00 in the morning, and the length of the branches is 3-5 cm.

[0011] In some other embodiments, disinfection is performed in step S1 or S2; the concentration of the disinfectant is 0.5-2 g / L.-1 The disinfection time is 1-10 min;

[0012] Preferably, the concentration of the disinfectant is 1 g·L -1 The disinfection time is 1 min.

[0013] In some other embodiments, the disinfectant used in the disinfection treatment in step S1 or S2 is a disinfectant mixed from Bacillus licheniformis and Bacillus subtilis or carbendazim.

[0014] In some other embodiments, the mass ratio of the Bacillus licheniformis and Bacillus subtilis is 1:(1-5);

[0015] Preferably, the mass ratio of the Bacillus licheniformis and Bacillus subtilis is 1:3.

[0016] In some other embodiments, in step S3, the rooting agent is one or both of indole-3-butyric acid and melatonin.

[0017] The concentration of the indole-3-butyric acid is 400-1200 mg·L -1 The concentration of the melatonin is 100-300 mg·L -1 .

[0018] In some other embodiments, in step S3, the rooting agent is a mixture of indole-3-butyric acid and melatonin, and the concentration ratio of the indole-3-butyric acid and the melatonin is 800 mg·L -1 :(100-300) mg·L -1 .

[0019] Preferably, the concentration ratio of the indole-3-butyric acid and the melatonin is 800 mg·L -1 :200 mg·L -1 .

[0020] In some other embodiments, in step S3, the substrate is nutrient soil, sandy soil, oak powder, perlite, coconut husk, and buckwheat husk.

[0021] In some other embodiments, in step S3, the substrate is nutrient soil+sandy soil+oak powder, nutrient soil+perlite+oak powder, nutrient soil+sandy soil+perlite, sandy soil+nutrient soil+coconut husk, sandy soil+nutrient soil+coconut husk, nutrient soil, nutrient soil+sandy soil+buckwheat husk, nutrient soil+sandy soil+buckwheat husk, nutrient soil+buckwheat husk, nutrient soil+buckwheat husk.

[0022] Preferably, the substrate is a mixture of nutrient soil + sand + oak powder in a mass ratio of 2:2:1, a mixture of nutrient soil + perlite + oak powder in a mass ratio of 2:2:1, a mixture of nutrient soil + sand + perlite in a mass ratio of 1:1:1, a mixture of sand + nutrient soil + coconut husk in a mass ratio of 1:1:1, a mixture of sand + nutrient soil + coconut husk in a mass ratio of 2:1:1, nutrient soil, a mixture of nutrient soil + sand + buckwheat husk in a mass ratio of 1:1:1, a mixture of nutrient soil + sand + buckwheat husk in a mass ratio of 1:1:2, a mixture of nutrient soil + buckwheat husk in a mass ratio of 1:1, or a mixture of nutrient soil + buckwheat husk in a mass ratio of 2:1.

[0023] Further preferably, the substrate is a mixture of nutrient soil + perlite + oak powder in a mass ratio of 2:2:1.

[0024] The beneficial effects of the present application are:

[0025] The Mongolian medicine plant artemisia dubia cuttage propagation method proposed in the present application exhibits significant beneficial effects in protecting the ecological environment, improving the propagation efficiency, enriching the germplasm resources, enhancing the stress resistance, and improving the economic benefits, and has important popularization and application value. Compared with the traditional wild resource picking and seed breeding method, it has significant advantages in many aspects, as follows:

[0026] (1) Environmental protection and sustainability: By cuttage propagation of artemisia dubia, the over-picking of wild resources is effectively avoided, and the damage to the environment caused by picking activities, such as soil erosion, grassland degradation, and desertification, is significantly reduced, which is conducive to the protection and restoration of the ecological environment, and realizes the sustainable utilization of Mongolian medicine resources.

[0027] (2) Improve the propagation efficiency: Compared with seed breeding, cuttage propagation has higher survival rate and growth rate. By optimizing the key technical links such as cuttage substrate, cuttage timing, hormone treatment, and subsequent management, the survival rate and growth quality of artemisia dubia cuttage seedlings are significantly improved, the growth cycle is shortened, and the propagation efficiency is greatly improved, which meets the market demand.

[0028] (3) Enrich the germplasm resources: Cuttage propagation can rapidly expand the population size while maintaining the excellent traits of the mother plant, avoiding the genetic drift and germplasm degradation problems that may occur in seed breeding. The application of this method helps to enrich the germplasm resource bank of artemisia dubia, and provides higher quality and more stable raw material sources for the Mongolian medicine industry.

[0029] (4) Enhance the stress resistance: Through scientific management and appropriate physiological regulation measures such as hormone treatment and nutrient supply, the stress resistance of artemisia dubia cuttage seedlings can be significantly improved, including drought resistance, cold resistance, disease and pest resistance, etc., so that they can maintain good growth status in different growth environments, improve yield and quality.

[0030] (5) Economic benefits are remarkable: due to the high efficiency and stability of the cutting propagation, the method can not only quickly increase the yield of artemisia vulgaris, but also reduce the production cost such as reducing the seed procurement cost, shortening the growth cycle to reduce the land and labor cost, thereby improving the economic benefits of the Mongolian medicine production enterprise and promoting the local economic development. BRIEF DESCRIPTION OF DRAWINGS

[0031] The drawings accompanying the specification of this application form a part thereof, serve to provide further understanding of the application, and together with the description of the exemplary embodiments of the application given below, explain the application. The present application is not intended to be unduly limited by such a specific embodiment.

[0032] Figure 1 A photo of the artemisia vulgaris cutting seedling in the embodiment of the application;

[0033] Figure 2 A photo of the artemisia vulgaris cutting substrate in the embodiment of the application;

[0034] Figure 3 A picture of the fermentation product of the dry leaves and seeds of quercus mongolica in the embodiment of the application. DETAILED DESCRIPTION

[0035] All raw materials and reagents used in the application are commercially available.

[0036] The application provides a method for cutting propagation of the Mongolian medicine plant artemisia vulgaris, comprising the following steps:

[0037] S1, disinfecting the artemisia vulgaris branches;

[0038] S2, pruning the artemisia vulgaris branches after disinfection, and obtaining artemisia vulgaris cutting segments after re-disinfection;

[0039] S3, treating the artemisia vulgaris cutting segments with a rooting agent, and cutting the artemisia vulgaris cutting segments in the substrate for cutting propagation.

[0040] The scheme of the application will be further described in detail in combination with the specific embodiments, as follows:

[0041] Example 1

[0042] A method for cutting propagation of the Mongolian medicine plant artemisia vulgaris, which studies the influence of different seedling ages on the rooting rate and survival rate of cutting, and the specific operation steps are as follows:

[0043] S1: Pick up different age of Artemisia princeps stems, choose sunny weather in the morning 9 o'clock to 11 o'clock, choose the degree of higher lignification part of the branch with growth time for 60d above, branch length 3-5cm. Adopt different disinfectant to pick up Artemisia princeps branch treatment 10min, prevent the cut surface from being infected by bacteria, cause black rod and other diseases and can't root.

[0044] S2: The step S1 disinfection after Artemisia princeps branch is cut to branch treatment, remove old branch, old leaf, disease leaf. After cutting treatment, disinfect again;

[0045] S3: The step S2 is handled after different age of mother plant is grafted to the matrix for nutrient soil+pearl stone+vermiculite (mixing mass ratio is 1:1:1). Matrix grafts 30 grafts, repeats 3 times experiments.

[0046] The kind of disinfectant used in step S1 and S2 to the influence of grafting seedling resistance is researched as shown in table 1. From table 1, it can be seen that the best soil disinfectant is Hartz wood + bacillus subtilis (mixing mass ratio 1:3, namely 0.25g Hartz wood + 0.75g bacillus subtilis in 1L water), which increases the resistance of grafting seedling. This is because Hartz wood has strong mycelium growth and spread ability, and the sterilization function of parasitic disease bacteria, effectively competes with pathogenic bacteria for nutrition and space, thereby reducing the invasion of pathogenic bacteria on Artemisia princeps cutting seedlings. The antibiotics and biological enzymes and active substances secreted by Hartz wood can stimulate plant cell growth and development, improve the resistance of Artemisia princeps cutting seedlings; The induced resistance ability of Hartz wood can also make Artemisia princeps cutting seedlings show stronger resistance when facing adversity. Although bacillus subtilis can also produce antibiotics and plant growth regulators to inhibit pathogenic bacteria and promote plant growth, its effect is relatively mild, not as strong as Hartz wood. Bacillus subtilis mainly improves the soil environment and provides nutrients to indirectly improve the resistance of Artemisia princeps cutting seedlings.

[0047] In the proportion of Hartz wood + bacillus subtilis mixed mass ratio 1:3, the number of Hartz wood is relatively small, but due to its strong mycelium growth and spread ability, sterilization function of parasitic disease bacteria and induced resistance ability, it still plays an important role in enhancing the resistance of Artemisia princeps cutting seedlings. Although the number of bacillus subtilis is relatively large, its effect is relatively mild, mainly through improving the soil environment and providing nutrients to play a role. Therefore, in the proportion of Hartz wood + bacillus subtilis mixed mass ratio 1:3, Hartz wood may perform more outstanding in the performance of enhancing the resistance of Artemisia princeps cutting seedlings.

[0048] Table 1 Effect of different soil disinfectants on the resistance of cutting seedlings

[0049]

[0050] The effects of different seedling ages on rooting rate and survival rate of cuttings are shown in Table 2. As can be seen from Table 2, there are significant differences in rooting time, rooting rate, total root number, and root length between two-year-old and three-year-old Artemisia vulgaris seedlings. As a perennial herb, the biological characteristics and growth patterns of Artemisia vulgaris are important factors affecting the survival rate of cuttings. Generally, two-year-old plants have stronger regenerative ability and adaptability than three-year-old plants. This is mainly due to the fact that young plants contain fewer root inhibiting substances, and nutrients are mainly used for vegetative growth, so the rooting ability and survival rate of their branches are usually higher.

[0051] Table 2 Effects of different seedling ages on rooting rate and survival rate of cuttings

[0052]

[0053] Two-year-old Artemisia vulgaris plants usually have more tender cuttings with active cell division and higher physiological metabolism. This vitality helps the cuttings quickly adapt to the new environment after cutting and start the rooting process. In contrast, the cuttings of three-year-old plants may have weakened cell division ability and reduced physiological metabolism due to aging, leading to decreased rooting ability.

[0054] Two-year-old plant cuttings usually contain more nutrients such as soluble sugars and amino acids, which are essential for cell division and growth during the rooting process. As the age of the plant increases, the content of these nutrients may gradually decrease, affecting the rooting ability of the cuttings.

[0055] Three-year-old plant cuttings may contain more root inhibiting substances that inhibit the initiation and progress of the rooting process. In contrast, young plant cuttings have lower levels of root inhibiting substances, which are more conducive to the progress of the rooting process.

[0056] Young plants (such as two-year-old) usually have stronger environmental adaptability, which can quickly adapt to new soil and climate conditions such as Figure 1 This helps the cuttings quickly root and grow after cutting, thereby increasing the survival rate. Therefore, two-year-old Artemisia vulgaris seedlings are the best cutting material.

[0057] Example 2

[0058] Hart's wood raspberry + Bacillus subtilis (mixed mass ratio 1:3, i.e. 0.25 g Hart's wood raspberry + 0.75 g Bacillus subtilis in 1 L water) were used as disinfectants, and two-year-old Artemisia vulgaris seedlings were the best cutting material. The operation processes of steps S1 and S2 were the same as in Example 1. The difference between Example 1 and Example 2 is that different substrates were used to treat Artemisia vulgaris branches in step S3.

[0059] Specifically, in step S3, different substrates were used to treat the Artemisia argyi branches. The substrates used are as follows (the mixing ratio of each component is 2:2:1): nutrient soil + sand + oak powder, nutrient soil + perlite + oak powder, nutrient soil + sand + perlite, sand + nutrient soil + coconut husk, sand + nutrient soil + coconut husk, nutrient soil, nutrient soil + sand + buckwheat skin, nutrient soil + sand + buckwheat skin, nutrient soil + buckwheat skin, and nutrient soil + buckwheat skin. Each group of substrates had 30 cuttings, and the experiment was repeated three times. The results are shown in Table 3. As shown in Table 3, the best substrate was nutrient soil + perlite + oak powder (2:2:1), in which the oak powder was the product of fermentation of crushed Mongolian oak leaves and seeds as shown in Figure 3 The Mongolian oak leaves and seeds contain rich organic matter and various mineral elements, which are decomposed into forms more easily absorbed by plants after fermentation.

[0060] Organic matter can provide carbon and energy sources for plants, improve soil structure, and increase soil water retention and air permeability. Mineral elements are essential for the normal growth and development of plants and have a positive effect on promoting the rooting of Artemisia argyi cuttings. During fermentation, some components in Mongolian oak leaves and seeds may be converted into plant growth regulators, such as gibberellins and cytokinins. These growth regulators can promote plant cell division and growth, thereby benefiting the rooting of Artemisia argyi cuttings.

[0061] The fermentation product contains various enzymes, such as cellulase and protease. These enzymes can decompose organic matter in the soil, release nutrients for plant absorption and utilization, and promote plant cell division and growth.

[0062] Adding fermentation products can improve soil structure, increase soil porosity and air permeability, which is beneficial to the respiration and water absorption of the root system of Artemisia argyi cuttings, promoting rooting.

[0063] The fermentation product can also regulate the pH of the soil, and appropriate soil pH is conducive to the absorption and utilization of nutrients by plants, thereby promoting rooting. These effects collectively promote the development and growth of the root system of Artemisia argyi cuttings, improving their survival rate and stress resistance as shown in Figure 2

[0064] Table 3 Effect of different substrates on the rooting rate and survival rate of cuttings

[0065]

[0066] Example 3

[0067] ​Two-year-old small white wormwood seedlings were selected as the best cutting material, and nutrient soil + perlite + oak powder (2:2:1) was the best substrate. The operation processes of steps S1 and S2 were the same as those of Example 1. Different from Example 1, step S3 was to dip the small white wormwood branches in different rooting agents for 10 seconds before cutting.

[0068] The rooting agents and concentrations (mg·L -1 ) used in step S3 are as follows: IBA 400, IBA 800, IBA 1200, melatonin 100, melatonin 200, melatonin 300, IBA 800 + melatonin 100, IBA 800 + melatonin 200, IBA 800 + melatonin 300; wherein IBA is indole-3-butyric acid. The research results are shown in Table 4. As can be seen from Table 4, IBA 800 mg / l + melatonin 200 mg / l is the best rooting agent combination. IBA and melatonin have complementary effects in promoting plant rooting. IBA mainly promotes rooting by promoting cell division and differentiation, increasing nutrient accumulation, and regulating enzyme activity; while melatonin plays a role by removing free radicals, antioxidant, regulating hormone balance and improving stress resistance. The synergistic effect of the two can more effectively promote the rooting of small white wormwood cutting seedlings.

[0069] IBA and melatonin have synergistic effects in regulating plant physiological processes. They can jointly regulate metabolic activities in the plant body, promote the accumulation and distribution of nutrients, and provide sufficient energy and material basis for rooting. Through the synergistic effect of IBA and melatonin, the rooting quality and speed of small white wormwood cutting seedlings can be significantly improved. This is not only manifested in the increase in the number of roots, but also in the aspects of thick and vigorous root growth, etc.

[0070] Table 4 Effects of different rooting agents on rooting rate and survival rate of cuttings

[0071]

[0072] The above only describes the preferred embodiments of the present application and is not used to limit the present application. For those skilled in the art, the present application can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A method for propagating the Mongolian medicinal plant Artemisia annua by cuttings, characterized in that, Includes the following steps: S1. Disinfect the Artemisia argyi branches; the Artemisia argyi branches are two to three years old Artemisia argyi branches that have grown for more than 60 days; the Artemisia argyi branches are harvested between 9 am and 11 am, and the branches are 3-5 cm long. S2, after disinfection, the branches of Artemisia argyi were pruned and disinfected again to obtain Artemisia argyi cuttings; S3, the Artemisia annua cuttings are treated with a rooting agent, and the rooted Artemisia annua cuttings are then inserted into a substrate for propagation; the substrate consists of nutrient soil, sand, oak powder, perlite, coconut coir and buckwheat hulls; The disinfectant used in step S1 or S2 is a mixture of Rhizopus harzianum and Bacillus subtilis or carbendazim. In step S3, the rooting agent is indolebutyric acid and melatonin; the concentration of indolebutyric acid is 400-1200 mg·L⁻¹. -1 The concentration of melatonin is 100-300 mg·L. -1 ; In step S3, the oak powder is a product of fermentation after crushing Mongolian oak leaves and seeds.

2. The method for propagating the Mongolian medicinal plant Artemisia annua by cuttings according to claim 1, characterized in that, In step S1, the Artemisia argyi branch is a two-year-old Artemisia argyi branch that has grown for more than 60 days.

3. The method for propagating the Mongolian medicinal plant Artemisia annua by cuttings according to claim 1, characterized in that, Disinfection treatment is performed in step S1 or S2; the concentration of the disinfectant is 0.5-2 g·L. -1 The disinfection time is 1-10 minutes.

4. The method for propagating the Mongolian medicinal plant Artemisia annua by cuttings according to claim 3, characterized in that, The concentration of the disinfectant is 1 g·L. -1 The disinfection time is 1 minute.

5. The method for propagating the Mongolian medicinal plant Artemisia annua by cuttings according to claim 1, characterized in that, The mass ratio of the mixed Rhizophora harzianum and Bacillus subtilis is 1:(1-5).

6. The method for propagating the Mongolian medicinal plant Artemisia annua by cuttings according to claim 1, characterized in that, The mass ratio of the mixed Rhizophora harzianum and Bacillus subtilis is 1:

3.

7. The method for propagating the Mongolian medicinal plant Artemisia annua by cuttings according to claim 1, characterized in that, In step S3, the rooting agent is a mixture of indolebutyric acid and melatonin, wherein the concentration ratio of indolebutyric acid to melatonin is 800 mg·L⁻¹. -1 (100-300) mg·L -1 .

8. The method for propagating the Mongolian medicinal plant Artemisia annua by cuttings according to claim 1, characterized in that, The concentration ratio of indolebutyric acid to melatonin is 800 mg·L⁻¹. -1 200 mg·L -1 .

9. The method for propagating the Mongolian medicinal plant Artemisia annua by cuttings according to claim 1, characterized in that, In step S3, the substrate is nutrient soil + sand + oak powder, nutrient soil + perlite + oak powder, nutrient soil + sand + perlite, sand + nutrient soil + coconut coir, sand + nutrient soil + coconut coir, nutrient soil, nutrient soil + sand + buckwheat hulls, nutrient soil + sand + buckwheat hulls, nutrient soil + buckwheat hulls, and nutrient soil + buckwheat hulls.

10. The method for propagating the Mongolian medicinal plant Artemisia annua by cuttings according to claim 1, characterized in that, The substrate is a mixture of nutrient soil, sand, and oak powder in a mass ratio of 2:2:1; nutrient soil, perlite, and oak powder in a mass ratio of 2:2:1; nutrient soil, sand, and perlite in a mass ratio of 1:1:1; sand, nutrient soil, and coconut coir in a mass ratio of 1:1:1; sand, nutrient soil, and coconut coir in a mass ratio of 2:1:1; nutrient soil, nutrient soil, sand, and buckwheat hulls in a mass ratio of 1:1:1; nutrient soil, sand, and buckwheat hulls in a mass ratio of 1:1:2; nutrient soil and buckwheat hulls in a mass ratio of 1:1; and nutrient soil and buckwheat hulls in a mass ratio of 2:

1.

11. The method for propagating the Mongolian medicinal plant Artemisia annua by cuttings according to claim 1, characterized in that, The substrate is a mixture of nutrient soil, perlite, and oak powder in a mass ratio of 2:2:1.

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