Detection method and detection system for physiological information of experimental animal

Abstract

The invention discloses a method for detecting physiological information of an experimental animal. The method comprises the following steps: collecting real-time physiological information: collecting the real-time physiological information of the experimental animal; tobacco smoke exposure: exposing the experimental animals in the tobacco smoke in the process of collecting the real-time physiological information; collecting the action time of the tobacco smoke: collecting the action starting time of the tobacco smoke on the experimental animal and the action ending time of the tobacco smoke on the experimental animal; and information fusion: fusing the real-time physiological information of the experimental animal with the action starting time and the action ending time to obtain synchronous physiological information. According to the detection method provided by the invention, the time when the smoke reaches and stops reaching the experimental animal is accurately collected, the real-time physiological information of the experimental animal exposed in the tobacco smoke is obtained, and experimental data support is provided for subsequent accurate analysis of instantaneous or short-time physiological effects caused after the tobacco smoke reaches the experimental animal. The invention provides a detection system for physiological information of an experimental animal.

Description

Technical Field

[0001] The invention relates to the technical field of tobacco smoke experimental animal exposure experiments, and in particular to a detection method and a detection system for experimental animal physiological information. Background Art

[0002] Smoke exposure devices, such as smoke exposure towers, are mainly used to expose the mouths and noses of experimental animals to the smoke in the smoke exposure tower for related experiments, and are widely used in the field of animal physiological effect research related to tobacco products.

[0003] The existing smoke exposure tower series experiments tie up awake experimental animals in a fixture and then expose them to tobacco smoke. During the period of exposure to tobacco smoke, the physiological information of the experimental animals on the outside of their body surface is continuously observed and collected. Specifically, the behavior of the animals is observed and recorded, such as the movement trajectory of the experimental animals, the experimental animals touching their noses with their paws, and other behavioral information of the experimental animals that can be observed on the outside of their body surface. Alternatively, the experimental animals are tied up in a fixture and then continuously exposed to tobacco smoke for a period of time, and then the physiological information of the experimental animals on the inside of their body surface is collected, such as the physiological information of the brain area activity of the experimental animals.

[0004] At present, the smoke exposure tower series experiments collect physiological information from the inner surface of the experimental animals, mainly for the experimental animals to be exposed to tobacco smoke for a period of time, and then conduct subsequent experimental tests, which can meet certain experimental design requirements. However, the physiological information of the experimental animals is not combined with the time and environmental information, making it impossible to analyze the real-time impact of the real-time environment of the experimental animals on their physiological information. Summary of the invention

[0005] The purpose of the present invention is to solve the problem that existing experiments do not combine the physiological information of experimental animals with the time and environmental information, resulting in the inability to accurately analyze and determine the real-time impact of the real-time environment (such as tobacco smoke environment) of the experimental animals on their physiological information.

[0006] In the first aspect, the method for detecting the physiological information of experimental animals provided by the present invention collects the time and tobacco smoke environment information of the experimental animals, and obtains the real-time physiological information of the experimental animals exposed to tobacco smoke, thereby combining the physiological information of the experimental animals with the time, environment and other information, so as to subsequently accurately analyze the real-time impact of the real-time environment of the experimental animals on their physiological information.

[0007] To solve the above technical problems, an embodiment of the present invention discloses a method for detecting physiological information of experimental animals, including: collecting real-time physiological information: collecting real-time physiological information of experimental animals; tobacco smoke exposure: exposing experimental animals to tobacco smoke during the process of collecting real-time physiological information; collecting tobacco smoke action time: collecting the start time and end time of tobacco smoke action on experimental animals; information fusion: fusing the real-time physiological information of experimental animals with the start time and end time of action to obtain synchronous physiological information.

[0008] By adopting the above technical scheme, the method for detecting physiological information of experimental animals provided by the present invention collects the time when smoke reaches the experimental animals, and obtains real-time physiological information of the experimental animals exposed to tobacco smoke, thereby providing experimental data support for the subsequent accurate analysis of real-time physiological changes of experimental animals exposed to tobacco smoke.

[0009] According to another specific embodiment of the present invention, the method for detecting physiological information of experimental animals also includes: collecting tobacco smoke concentration information exposed to the experimental animals at least from the start time to the end time; fusing the real-time physiological information of the experimental animals, the start time, the end time and the tobacco smoke concentration information to obtain concentration-synchronized physiological information.

[0010] According to another specific embodiment of the present invention, the method for detecting physiological information of experimental animals further includes: inputting air of different flow rates into the tobacco smoke from the start time to the end time to adjust the concentration of the tobacco smoke exposed to the experimental animals.

[0011] According to another specific embodiment of the present invention, a smoke detector is used to detect tobacco smoke; the start time is the time when the smoke detector detects tobacco smoke, and the end time is the time when the smoke detector cannot detect tobacco smoke, or the start time is the time when the smoke detector detects that the concentration of tobacco smoke exceeds a preset initial threshold, and the end time is the time when the smoke detector detects that the concentration of tobacco smoke is lower than a preset end threshold.

[0012] According to another specific embodiment of the present invention, the smoke detector is arranged at the mouth and nose of the experimental animal, or the smoke detector is arranged at the outlet where the tobacco smoke is released, and the experimental animal is located at the outlet.

[0013] According to another specific embodiment of the present invention, tobacco smoke exposure is performed multiple times, and the start time and end time of tobacco smoke exposure are collected each time.

[0014] According to another specific embodiment of the present invention, the experimental animals include macaques, rabbits or pikas, cats or dogs, mice or rats or golden hamsters or guinea pigs or gerbils or black-striped mice.

[0015] According to another specific embodiment of the present invention, the real-time physiological information includes electromyographic physiological information and / or the release level of dopamine in the brain.

[0016] In the second aspect, an embodiment of the present invention discloses a physiological information detection system for experimental animals, which is used to implement the physiological information detection method for experimental animals of any of the aforementioned embodiments, including: a tobacco smoke exposure device, which is used to provide a tobacco smoke environment; an experimental chamber, which is used to place experimental animals, and the experimental chamber is connected to the tobacco smoke exposure device; a physiological information collector, which is used to collect real-time physiological information of experimental animals; a smoke recorder, which is used to collect the start time and end time of the tobacco smoke on the experimental animals; the detection system is used to merge the real-time physiological information of the experimental animals with the start time and the end time of the tobacco smoke on the experimental animals to obtain synchronized physiological information.

[0017] By adopting the above technical scheme, the experimental animal physiological information detection system collects the time when the smoke reaches the mouth and nose of the experimental animals, and obtains the real-time physiological information of the experimental animals exposed to tobacco smoke, thereby combining the physiological information of the experimental animals with the time, environment and other information in which they are located, so as to facilitate the subsequent accurate analysis of the real-time impact of the real-time environment of the experimental animals on their physiological information.

[0018] According to another specific embodiment of the present invention, the smoke recorder is used to collect tobacco smoke concentration information exposed to experimental animals at least from the start time to the end time; the detection system is used to fuse the real-time physiological information, the start time, the end time and the tobacco smoke concentration information of the experimental animals to obtain concentration-synchronized physiological information.

[0019] According to another specific embodiment of the present invention, the experimental animal physiological information detection system also includes an air conveyor, one end of which is connected to the tobacco smoke exposure device through an air conveying pipe, and the air conveyor is used to input air with different flow rates into the tobacco smoke exposure device from the start time to the end time to adjust the concentration of tobacco smoke exposed to the experimental animals.

[0020] According to another specific embodiment of the present invention, the smoke recorder includes: a smoke detector for detecting tobacco smoke; and a single-chip microcontroller for recording time.

[0021] According to another specific embodiment of the present invention, the smoke detector is provided with a first signal output terminal for outputting tobacco smoke information detected by the smoke detector; the single-chip microcontroller is provided with a signal receiving terminal, a signal processing unit and a second signal output terminal, wherein the signal receiving terminal is connected to the first signal output terminal for receiving tobacco smoke information output by the first signal output terminal; the signal processing unit is used to determine the start time and the end time of the action according to the tobacco smoke information and the time information to form the smoke time information; the second signal output terminal is connected to one end of the physiological information collector for outputting the smoke time information to the physiological information collector; the physiological information collector is connected to the experimental animal.

[0022] The experimental animal physiological information detection system also includes a system controller, which is connected to the second signal output terminal and one end of the physiological information collector, respectively, and is used to receive the smoke time information output by the second signal output terminal and the real-time physiological information collected by the physiological information collector. According to another specific embodiment of the present invention, the tobacco smoke exposure device is provided with an outlet, and the outlet is connected to the experimental chamber so that the experimental animal is placed at the outlet.

[0023] According to another specific embodiment of the present invention, the experimental animal physiological information detection system also includes: a smoking machine for generating tobacco smoke; the tobacco smoke exposure device is provided with an inlet connected to the smoking machine for receiving the tobacco smoke generated by the smoking machine.

[0024] According to another specific embodiment of the present invention, the experimental animal physiological information detection system also includes: a ventilation hood, a smoke detector is arranged inside the ventilation hood; a support member, one end of the support member is connected to the outlet of the tobacco smoke exposure device, and the other end is connected to the ventilation hood, and the ventilation hood is fixed inside the outlet through the support member. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 The process of the method for detecting physiological information of experimental animals in one embodiment of the present invention is shown as follows Figure 1 ;

[0026] Figure 2 The process of the method for detecting physiological information of experimental animals in one embodiment of the present invention is shown as follows Figure 2 ;

[0027] Figure 3 A schematic diagram showing synchronization of physiological information in an embodiment of the present invention;

[0028] Figure 4 A schematic diagram showing the change of smoke concentration at different times in one embodiment of the present invention;

[0029] Figure 5The signal acquisition of the smoke recorder under different air flow rates in one embodiment of the present invention is shown;

[0030] Figure 6 A schematic diagram showing the structure of an experimental animal physiological information detection system in one embodiment of the present invention;

[0031] Figure 7 A schematic structural diagram of a smoke recorder in an embodiment of the present invention is shown. DETAILED DESCRIPTION

[0032] The following specific embodiments illustrate the implementation of the present invention, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. Although the description of the present invention will be introduced in conjunction with the preferred embodiment, this does not mean that the features of this invention are limited to this implementation. On the contrary, the purpose of introducing the invention in conjunction with the implementation is to cover other options or modifications that may extend based on the claims of the present invention. In order to provide a deep understanding of the present invention, many specific details will be included in the following description. The present invention can also be implemented without using these details. In addition, in order to avoid confusion or blurring the focus of the present invention, some specific details will be omitted in the description. It should be noted that the embodiments of the present invention and the features in the embodiments can be combined with each other without conflict.

[0033] It should be noted that in this specification, similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it does not need to be further defined and explained in the subsequent drawings.

[0034] The terms “first”, “second”, etc. are only used for distinguishing descriptions and should not be understood as indicating or implying relative importance.

[0035] In the description of this embodiment, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this embodiment can be understood according to specific circumstances.

[0036] To make the objectives, technical solutions and advantages of the present invention more clear, the embodiments of the present invention will be further described in detail below with reference to the accompanying drawings.

[0037] First, refer to Figure 1 The present invention provides a method for detecting physiological information of experimental animals, comprising:

[0038] S1: Collect real-time physiological information: Collect real-time physiological information of experimental animals.

[0039] It should be noted that experimental animals (refer to national standard GB 14923-2010) include but are not limited to: macaques (primates), rabbits / ochotochalas (lagomorpha), cats / dogs (Carnivora), mice / rats / golden hamsters / guinea pigs / gerbils / apodemus (rodentia).

[0040] The real-time physiological information includes electromyographic physiological information and / or the release level of dopamine in the brain. For example, the electromyographic physiological information is laryngeal electromyographic physiological information. For example, the real-time monitoring and recording of the fluorescent signal of the release of dopamine neurotransmitters in the brain is achieved by in vivo fiber optic photometry.

[0041] For example, real-time physiological information of experimental animals is collected, such as Figure 3 shown. Figure 3 Taking the collected electromyographic signals of experimental animals as an example, the changes in the real-time physiological information of experimental animals within a period of time (within 9000 milliseconds) are shown. Within 0 milliseconds to 2000 milliseconds, the electromyographic signals of experimental animals generally show a downward trend, that is, the electromyographic signals are becoming less and less active. Within 2000 milliseconds to 3000 milliseconds, the electromyographic signals of experimental animals show an upward trend, that is, the electromyographic signals are becoming more and more active, and the value of the most active electromyographic signals in this stage is significantly higher than the initial active value of the electromyographic signals at 0 milliseconds. Within 3000 milliseconds to about 7800 milliseconds, the electromyographic signals of experimental animals generally show a downward trend, that is, the electromyographic signals are becoming less and less active. At about 7800 milliseconds to 8000 milliseconds, the electromyographic signals of experimental animals generally show an upward trend, and the value of the most active electromyographic signals in this stage is significantly lower than the initial active value of the electromyographic signals at 0 milliseconds. At 8000 milliseconds to 9000 milliseconds, the electromyographic signals of experimental animals generally show a downward trend and gradually tend to 0.

[0042] S2: Tobacco smoke exposure: Experimental animals are exposed to tobacco smoke while collecting real-time physiological information.

[0043] The real-time physiological information of the experimental animals collected according to step S2 includes, but is not limited to, the real-time physiological information before exposure to tobacco smoke, the real-time physiological information during exposure to tobacco smoke, and the real-time physiological information after cessation of exposure to tobacco smoke, so as to facilitate the subsequent analysis of the impact of tobacco smoke on the real-time physiological information of the experimental animals.

[0044] S3: Collecting the action time of tobacco smoke: Collecting the starting time and ending time of the action of tobacco smoke on experimental animals.

[0045] Through step S3, the action time of tobacco smoke (including the start time and end time of action on the experimental animals) is introduced into the process of collecting the real-time physiological information of the experimental animals, thereby realizing real-time collection and accurate recording of the action time of tobacco smoke and the real-time physiological information of the experimental animals.

[0046] Exemplarily, the tobacco smoke exposure time is collected as follows: Figure 3 Specifically, on the time axis shown on the horizontal axis, the black shaded portion indicates the start time, end time and duration time of tobacco smoke on experimental animals, also known as the marking signal. Among them, the 2000th millisecond is the start time of tobacco smoke on experimental animals, the 4000th millisecond is the end time of tobacco smoke on experimental animals, and the duration time of tobacco smoke on experimental animals is 2000 milliseconds.

[0047] In some other possible embodiments, there are multiple marking signals, and a marking signal is performed at a certain interval. Figure 5 As shown, a marking signal is sent every 30 seconds.

[0048] S4: Information fusion: The real-time physiological information of experimental animals is integrated with the start time and end time of the action to obtain synchronous physiological information.

[0049] The synchronous physiological information obtained includes the real-time physiological information of the experimental animals and the action time of tobacco smoke, which is convenient for observing and analyzing the instantaneous or short-term physiological effects caused by tobacco smoke reaching the experimental animals.

[0050] For example, the synchronous physiological information obtained is as follows: Figure 3 As mentioned earlier, Figure 3 It includes both the real-time physiological information of experimental animals and the start and end time of the effects of tobacco smoke on experimental animals.

[0051] By adopting the above technical scheme, the method for detecting the physiological information of experimental animals provided by the present invention collects the time when the smoke reaches the experimental animals, specifically, collects the time when the tobacco smoke arrives and stops reaching the experimental animals, and obtains the real-time physiological information of the experimental animals exposed to the tobacco smoke, and fuses the aforementioned time information and real-time physiological information to obtain synchronous physiological information, thereby realizing the combination of the physiological information of the experimental animals with the time, environment and other information in which they are located, so as to facilitate the subsequent accurate analysis of the real-time impact of the real-time environment in which the experimental animals are located on their physiological information.

[0052] In some possible embodiments provided by the present invention, reference is made to Figure 2 , the detection methods of experimental animal physiological information also include:

[0053] S5: Collect information on the tobacco smoke concentration to which the experimental animals were exposed from at least the start time to the end time of exposure.

[0054] S6: The real-time physiological information, starting time, ending time and tobacco smoke concentration information of the experimental animals are integrated to obtain concentration-synchronized physiological information.

[0055] In this embodiment, different concentrations of tobacco smoke are reflected by signals with different voltage intensities.

[0056] In this embodiment, on the basis of the aforementioned steps S1 to S4, step S5 is executed to collect the concentration information of tobacco smoke, and step S6 is executed to integrate the various information in the aforementioned step S4, namely, the real-time physiological information of the experimental animals, the start time and the end time of the tobacco smoke, to obtain concentration-synchronized physiological information, so as to observe and analyze the influence of the concentration of tobacco smoke on the physiological effects of experimental animals.

[0057] refer to Figure 4 , Figure 4 The schematic diagram of the change of tobacco smoke concentration at different times is shown. Specifically, before 2000 milliseconds, the tobacco smoke concentration is 0ppm. From 2000 milliseconds to about 3600 milliseconds, the tobacco smoke concentration gradually rises, and reaches a maximum concentration of about 1500ppm at about 3600 milliseconds. By closing the smoke release or by controlling the air flow rate entering the tobacco smoke, the tobacco smoke concentration gradually decreases from about 3600 milliseconds to 9000 milliseconds, and the rate of decrease is significantly slowed down, until the concentration of the tobacco smoke is reduced to 400ppm at 9000 milliseconds.

[0058] refer to Figure 3 and Figure 4 Combined with Figure 2 , integrating the real-time physiological information of experimental animals, the start time of action, the end time of action and the concentration of tobacco smoke, to obtain concentration-synchronized physiological information. Figure 3 and Figure 4, before 2000 milliseconds, the concentration of tobacco smoke was 0ppm, and the electromyographic signals of the experimental animals gradually changed from active to calm. At 2000 milliseconds, the concentration of tobacco smoke began to gradually increase, and the starting time of tobacco smoke's effect on the experimental animals was set to 2000 milliseconds. Correspondingly, the electromyographic signals of the experimental animals became more and more active from 2000 milliseconds. At about 3600 milliseconds, the concentration of tobacco smoke reached its maximum, and accordingly, the electromyographic signals of the experimental animals reached their most active at about 3600 milliseconds. From about 3600 milliseconds to 4000 milliseconds, the concentration of tobacco smoke gradually decreased, and the electromyographic signals of the experimental animals became less and less active. The 4000 millisecond was taken as the end time of tobacco smoke's effect on the experimental animals. From 4000 milliseconds to 9000 milliseconds, the concentration of tobacco smoke gradually decreased, and the electromyographic signals of the experimental animals became less and less active overall. Combined with Figure 3 and Figure 4 It can be clearly seen that the concentration of tobacco smoke has a synchronous effect on the real-time physiological information of experimental animals: the higher the concentration of tobacco smoke, the more active the real-time physiological information of experimental animals; the lower the concentration of tobacco smoke, the less active the real-time physiological information of experimental animals.

[0059] In some possible embodiments provided by the present invention, the method for detecting physiological information of experimental animals further includes: inputting air at different flow rates into the tobacco smoke from the start time to the end time to adjust the concentration of the tobacco smoke exposed to the experimental animals.

[0060] refer to Figure 5 In this embodiment, the concentration of tobacco smoke is diluted by inputting air of different flow rates into tobacco smoke. Exemplarily, the air flow rates are 0L / min, 0.2L / min, 0.5L / min, 1L / min, 3L / min, 5L / min, 10L / min, and 20L / min, respectively. The higher the air flow rate, the greater the dilution ratio of the concentration of tobacco smoke, that is, when the air flow rate is 0L / min, the concentration of tobacco smoke is the largest, and when the air flow rate is 20L / min, the concentration of tobacco smoke is the smallest. Exemplarily, the duration of each flow rate is 2 seconds, that is, the duration of 0L / min, 0.2L / min, 0.5L / min, 1L / min, 3L / min, 5L / min, 10L / min, and 20L / min is 2 seconds. It is sufficient to reduce the concentration of tobacco smoke to the target concentration by controlling the duration of each flow rate. If the duration of 1L / min of air is 3 seconds, the duration of 3L / min of air is 1 second.

[0061] Figure 5It is shown that at air flow rates of 0 L / min, 0.2 L / min, 0.5 L / min, 1 L / min, 3 L / min, 5 L / min, 10 L / min, and 20 L / min, the smoke recorder can output a marking signal every 30 seconds according to the collected tobacco smoke. In some other possible embodiments, the smoke recorder can output a marking signal every 10 seconds or every 20 seconds according to the collected tobacco smoke. The present invention does not limit the interval time.

[0062] In some possible embodiments provided by the present invention, a smoke detector is used to detect tobacco smoke. The start time is the time when the smoke detector detects tobacco smoke, and the end time is the time when the smoke detector cannot detect tobacco smoke, or the start time is the time when the smoke detector detects that the concentration of tobacco smoke exceeds a preset initial threshold, and the end time is the time when the smoke detector detects that the concentration of tobacco smoke is lower than a preset end threshold.

[0063] Specifically, in some possible embodiments, a smoke detector is used to detect tobacco smoke. The start time is the time when the smoke detector can detect tobacco smoke, and the end time is the time when the smoke detector cannot detect tobacco smoke. The smoke detector can sense the presence or absence of tobacco smoke, and the time when the presence or absence of tobacco smoke is detected is used as the start time and the end time.

[0064] In some other possible embodiments, a smoke detector is used to detect tobacco smoke. The start time is the time when the smoke detector detects that the concentration of tobacco smoke exceeds a preset initial threshold, and the end time is the time when the smoke detector detects that the concentration of tobacco smoke is lower than a preset end threshold. That is, the start time and the end time are determined based on whether the concentration of tobacco smoke detected by the smoke detector reaches a preset concentration threshold.

[0065] For example, reference Figure 3 and Figure 4 The start time is the time when the smoke detector detects that the concentration of tobacco smoke exceeds the preset initial threshold. If the preset initial threshold of tobacco smoke concentration is set to 0ppm, the start time is 2000 milliseconds. The end time is the time when the smoke detector detects that the concentration of tobacco smoke is lower than the preset end threshold. If the preset end threshold of tobacco smoke concentration is set to 1300ppm, the end time is 4000 milliseconds.

[0066] In some other possible embodiments, the presence or absence of tobacco smoke and the concentration of tobacco smoke are detected by a smoke detector, and the start time and the end time of the action are customized.

[0067] In some possible embodiments provided by the present invention, the smoke detector is disposed at the mouth and nose of the experimental animal, or the smoke detector is disposed at the outlet where tobacco smoke is released, and the experimental animal is disposed at the outlet.

[0068] It is necessary to obtain the precise time when tobacco smoke reaches the mouth and nose of the experimental animal in order to observe the instantaneous or short-term changes in real-time physiological information caused by tobacco smoke reaching the mouth and nose of the experimental animal. Therefore, in some possible embodiments of the present invention, a smoke detector is arranged at the mouth and nose of the experimental animal. That is, the smoke detector is wearable, and the smoke detector is arranged at the mouth and nose of the experimental animal. The arrival time and end time of the tobacco smoke detected by the smoke detector are the time when the tobacco smoke actually arrives at the mouth and nose of the experimental animal and the time when it ends arriving at the mouth and nose of the experimental animal. With the above technical solution, the smoke detector is arranged at the mouth and nose of the experimental animal to obtain the precise time when the tobacco smoke arrives and ends arriving at the mouth and nose of the experimental animal.

[0069] Preferably, the experimental animal is located at the outlet where tobacco smoke is released, and the smoke detector is arranged at the outlet. In the previous embodiment, the smoke detector is arranged at the mouth and nose of the experimental animal, and the real-time physiological information of the experimental animal changes in order to adapt to the worn smoke detector, which is not accurate enough. In addition, the experimental animal itself is relatively free to move. Although tobacco smoke exposure is carried out in a relatively closed environment, the range of movement of the experimental animal itself is not restricted as much as possible. If the smoke detector is to be fixed on the experimental animal, the mobility of the experimental animal will be restricted.

[0070] Therefore, preferably, the mouth and nose of the experimental animal are exposed to the outlet where the tobacco smoke is released, and the smoke detector is set at the outlet. That is, the smoke detector is set closest to the mouth and nose of the experimental animal, so that the time when the tobacco smoke is collected by the smoke detector is closest to the time when the tobacco smoke reaches the mouth and nose of the experimental animal. That is, the time when the smoke detector collects the tobacco smoke or the time when the smoke detector collects the tobacco smoke reaching the preset initial threshold is used as the time when the tobacco smoke reaches the experimental animal, that is, the start time, and the time when the smoke detector does not collect the tobacco smoke or the time when the smoke detector collects the tobacco smoke reaching the preset end threshold is used as the time when the tobacco smoke stops reaching the experimental animal, that is, the end time. In this embodiment, the position of the smoke detector is fixed during each experiment to ensure that the detected tobacco smoke information will not be erroneous due to the change of the position of the smoke detector during each experiment.

[0071] By adopting the above technical solution, the time when the smoke detector detects the presence or concentration of tobacco smoke at the outlet is used as the start time or end time of the tobacco smoke's effect on the experimental animals. The time is standardized, and the time when the smoke arrives and stops reaching the experimental animals can be accurately collected.

[0072] In some possible embodiments provided by the present invention, tobacco smoke exposure is performed multiple times, and the start time and end time of each tobacco smoke exposure are collected. That is, a smoke concentration marking signal is collected at intervals. As described above, for example, Figure 5 It shows the tobacco smoke collected every 30 seconds at air flow rates of 0 L / min, 0.2 L / min, 0.5 L / min, 1 L / min, 3 L / min, 5 L / min, 10 L / min, and 20 L / min, and a marking signal is output every 30 seconds.

[0073] Second, reference Figure 6 The present invention provides an experimental animal physiological information detection system 1, which is used to implement the experimental animal physiological information detection method of any of the above embodiments. The experimental animal physiological information detection system 1 includes a tobacco smoke exposure device 2, an experimental chamber 3, a physiological information collector 4 and a smoke recorder 5.

[0074] Among them, the tobacco smoke exposure device 2 is used to provide an environment of tobacco smoke. The experimental chamber 3 is used to place experimental animals. The experimental chamber 3 is connected to the tobacco smoke exposure device 2, and tobacco smoke can flow into the experimental chamber 3 through the tobacco smoke exposure device 2. The physiological information collector 4 is used to collect real-time physiological information of experimental animals. The smoke recorder 5 is used to collect the start time and end time of tobacco smoke on experimental animals. The experimental animal physiological information detection system 1 is used to merge the real-time physiological information of experimental animals with the start time and end time of action to obtain synchronous physiological information.

[0075] By adopting the above technical scheme, the experimental animal physiological information detection system 1 introduces the real-time collection of the start and end time of tobacco smoke on experimental animals when collecting real-time physiological information of experimental animals, so that the physiological effects of experimental animals can be accurately observed when the experimental animals are exposed to the tobacco smoke exposure device 2 in related experiments, that is, the changes in real-time physiological information of experimental animals before, during and after exposure to tobacco smoke are observed, enriching and expanding the functions of the existing tobacco smoke exposure device 2, and providing experimental data support for the subsequent accurate analysis of real-time physiological changes of experimental animals exposed to tobacco smoke. The experimental animal physiological information detection system 1 is reasonably designed, efficient and convenient to use, and is suitable for animal experiments related to tobacco smoke exposure.

[0076] In the embodiment, the physiological information collector is a non-binding or implantable device, which inputs the signal in the experimental animal to various external devices through signal connections. It is mainly necessary to fix the signal collection end buried in the larynx or head to the experimental animal well. In the acquisition of laryngeal electromyographic signals, the electrode is mainly fixed to the subcutaneous muscle tissue through surgical sutures, and then guided to the surface of the skull through a subcutaneous channel, and the metal wire is fixed to the skull surface with dental cement; dopamine signal acquisition is mainly through the optical fiber connector buried in the brain, and the optical fiber connector is also fixed to the skull surface with cement; therefore, the signal acquisition end will basically not be misplaced or offset, ensuring the accuracy of the signal collection site.

[0077] In the embodiments, reference Figure 6 The tobacco smoke exposure device 2 is provided with a plurality of outlets, and the experimental animal physiological information detection system 1 is provided with a plurality of experimental chambers 3, each outlet being connected to an experimental chamber 3, so that the experimental animals in the experimental chambers 3 can be exposed at the outlet of the tobacco smoke exposure device 2, that is, the experimental animals in the experimental chambers 3 are located at the outlet where the tobacco smoke is released. Accordingly, the smoke recorder 5 can be located at the outlet of each tobacco smoke exposure device 2. Figure 6 As shown, the experimental animal is placed at one outlet of the tobacco smoke exposure device 2, and the outlet is also provided with a smoke recorder 5. The figure shows that the smoke recorder 5 is placed at another outlet of the tobacco smoke exposure device 2, and the outlet is also provided with an experimental animal.

[0078] In some possible embodiments provided by the present invention, the smoke recorder 5 is used to collect tobacco smoke concentration information of the experimental animals at least from the start time to the end time. The experimental animal physiological information detection system 1 is used to integrate the real-time physiological information, the start time, the end time and the tobacco smoke concentration information of the experimental animals to obtain concentration-synchronized physiological information. As mentioned above, reference Figure 3 and Figure 4 Combined with Figure 2 , integrating the real-time physiological information, the start time of action, the end time of action and the tobacco smoke concentration information of the experimental animals, and obtaining the concentration-synchronized physiological information. The details are as described above and will not be elaborated here.

[0079] In this embodiment, the single-chip microcontroller 58 outputs a signal with different voltage strengths to reflect different tobacco smoke concentrations.

[0080] In some other possible embodiments provided by the present invention, refer to Figure 6 The experimental animal physiological information detection system 1 includes an air inlet 21 and an outlet 22 .

[0081] The experimental animal physiological information detection system 1 also includes an air conveyor (not shown in the figure), one end of which is connected to the tobacco smoke exposure device 2 through an air conveying pipeline, and the air conveyor is used to input air of different flow rates into the tobacco smoke exposure device 2 from the start time to the end time to dilute the concentration of tobacco smoke exposed to the experimental animals. As mentioned above, illustratively, the air flow rates are 0L / min, 0.2L / min, 0.5L / min, 1L / min, 3L / min, 5L / min, 10L / min, and 20L / min, respectively. The higher the air flow rate, the greater the dilution ratio of the concentration of tobacco smoke, that is, at an air flow rate of 0L / min, the concentration of tobacco smoke is the largest, and at an air flow rate of 20L / min, the concentration of tobacco smoke is the smallest.

[0082] In some other possible embodiments provided by the present invention, the smoke recorder 5 includes a smoke detector 52 and a single-chip microcontroller 58. The smoke detector 52 is used to detect tobacco smoke; and the single-chip microcontroller 58 is used to record time.

[0083] In some other possible embodiments provided by the present invention, refer to Figure 7 The smoke detector 52 is provided with a first signal output terminal 55, which is used to output the tobacco smoke information detected by the smoke detector 52, and the tobacco smoke information includes the presence or absence of tobacco smoke and the concentration of tobacco smoke. The single-chip microcontroller 58 is provided with a signal receiving terminal 582, a signal processing unit (not shown in the figure) and a second signal output terminal 581. Among them, the signal receiving terminal 582 is connected to the first signal output terminal 55, and is used to receive the tobacco smoke information output by the first signal output terminal 55. The signal processing unit (not shown in the figure) is used to determine the start time and the end time of the action according to the tobacco smoke information and the time information, and form the smoke time information. The second signal output terminal 581 is connected to one end of the physiological information collector 4, and is used to output the smoke time information to the physiological information collector 4. The other end of the physiological information collector 4 is connected to the experimental animal, and is used to collect the physiological information of the experimental animal.

[0084] In this embodiment, the second signal output terminal 581 is connected to one end of the physiological information collector 4 through a data transmission line 583 to transmit the smoke time information to the physiological information collector 4 .

[0085] In some other possible embodiments provided by the present invention, the experimental animal physiological information detection system 1 also includes a system controller (not shown in the figure), which is respectively connected to the second signal output terminal 581 and one end of the physiological information collector 4, and is used to receive the smoke time information output by the second signal output terminal 581 and the real-time physiological information collected by the physiological information collector 4.

[0086] In some other possible embodiments, the second signal output terminal 581 is connected to a third-party device via a data transmission line 583, so that the second signal output terminal 581 transmits the collected start time and end time of action to the third-party device in the form of a marking signal. Specifically, the third-party device can display numerical information such as time information, concentration information, and real-time physiological information. Exemplarily, the third-party device is a third-party digital-to-analog converter or a signal recorder.

[0087] In some possible embodiments, one end of the physiological information collector 4 is connected to a third-party device, so that the real-time physiological information of the experimental animal collected by the physiological information collector 4 is transmitted to the third-party device.

[0088] In some possible embodiments, reference Figure 7 The smoke detector 52 is provided with a positive power pin 54 and a negative power pin 56. The positive power pin 54 is connected to the positive pole of the power interface of the single-chip microcontroller 58 through a connecting line 57, and the negative power pin 56 is connected to the negative pole of the power interface of the single-chip microcontroller 58 through a connecting line 57.

[0089] In some other possible embodiments provided by the present invention, the single-chip microcontroller 58 includes a program reading and writing module 59 for reading or writing programs corresponding to real-time physiological information, and the real-time physiological information includes electromyographic physiological information and / or the release level of dopamine in the brain.

[0090] In some other possible embodiments provided by the present invention, the tobacco smoke exposure device 2 is provided with an outlet, and the outlet is connected to the experimental chamber 3, and the experimental animal is placed at the outlet, and the mouth and nose of the experimental animal can be exposed to the tobacco smoke.

[0091] In some other possible embodiments provided by the present invention, the smoke detector 52 is arranged at the exit or the smoke detector 52 is arranged at the mouth and nose of the experimental animal. Preferably, the smoke detector 52 is arranged at the exit. See the above for details, which will not be repeated here.

[0092] In some other possible embodiments provided by the present invention, the experimental animal physiological information detection system 1 further includes a smoking machine 6 for generating tobacco smoke. The tobacco smoke exposure device 2 is provided with an inlet connected to the smoking machine 6 for receiving the tobacco smoke generated by the smoking machine 6.

[0093] refer to Figure 6 The experimental animal physiological information detection system 1 also includes a tobacco smoke delivery pipe 7, one end of which is connected to the inlet of the tobacco smoke exposure device 2, and the other end is connected to the smoking machine 6, for delivering the tobacco smoke generated by the smoking machine 6 to the tobacco smoke exposure device 2.

[0094] In some other possible embodiments provided by the present invention, refer to Figure 7 The experimental animal physiological information detection system 1 also includes a ventilation cover 53 and a support member 51.

[0095] The smoke detector 52 is arranged inside the ventilation cover 53. The smoke detector 52 is connected to the ventilation cover 53. Exemplarily, the smoke detector 52 is detachably connected to the ventilation cover 53. For example, the smoke detector 52 is fixed inside the ventilation cover 53 by bolts. The ventilation cover 53 has a ventilation function and is provided with at least two ventilation holes. Tobacco smoke from the tobacco smoke exposure device 2 can enter the ventilation cover 53 through one ventilation hole, flow through the smoke detector 52, and flow out of the ventilation cover 53 through another ventilation hole. The ventilation cover 53 facilitates the circulation of tobacco smoke so that the smoke detector 52 can accurately detect tobacco smoke. In some possible embodiments, one side of the ventilation cover 53 is open, and each side of the ventilation cover 53 is provided with a side opening.

[0096] refer to Figure 6 and Figure 7 , one end of the support member 51 is connected to the outlet of the tobacco smoke exposure device 2, and the other end is connected to the ventilation cover 53, and the ventilation cover 53 is fixed inside the outlet through the support member 51. Exemplarily, the support member 51 is fixedly connected to the ventilation cover 53 or detachably connected. For example, the support member 51 is welded to the ventilation cover 53 or the support member 51 is connected to the ventilation cover 53 by hot melt adhesive. The outlet of the tobacco smoke exposure device 2 is an outlet pipe, one end of the support member 51 is fixed to the inner wall of the outlet pipe of the tobacco smoke exposure device 2, and the other end is connected to the ventilation cover 53, so that the support member 51 fixes the ventilation cover 53 at the outlet of the tobacco smoke exposure device 2. On the other hand, the smoke detector 52 is arranged inside the ventilation cover 53 and connected to the ventilation cover 53, so that the smoke detector 52 is fixed at a fixed position of the outlet of the tobacco smoke exposure device 2, which is conducive to the tobacco smoke detected by the smoke detector 52 without error in each experiment.

[0097] In this embodiment, reference Figure 7 The support member 51 is welded to one side of the ventilation cover 53, and the smoke detector 52 is installed in the ventilation cover 53 by bolts. Pin pins are exposed on one side of the ventilation cover 53, the positive electrode of the power pin 54 is connected to the positive electrode of the power interface of the single-chip microcontroller 58 through the connecting line 57, the negative electrode of the power pin 56 is connected to the negative electrode of the power interface of the single-chip microcontroller 58 through the connecting line 57, and the first signal output terminal 55 is connected to the signal receiving terminal 582 of the single-chip microcontroller 8 through the connecting line 7.

[0098] The program read / write module 59 of the single-chip microcontroller 58 is connected to the computer to read and write the program, and at the same time, the 5V power supply is supplied through the program read / write module 59. That is, the program read / write module 59 of the single-chip microcontroller 58 is connected to the computer to supply power to the smoke recorder 5 and read and write the program. Exemplarily, the program read / write module 59 is a B-type USB interface.

[0099] The second signal output terminal 581 of the single-chip microcontroller 58 outputs a signal to the communication interface of the third-party device through the data transmission line 583, and then the written program is injected into the single-chip microcontroller 58 according to different experimental requirements, and subsequent experiments can be carried out. For example, the third-party device is other animal physiological effect recording equipment, and the data transmission line 583 is a BNC line.

[0100] When a certain concentration of smoke appears at the outlet of the tobacco smoke exposure device 2, the smoke detector 52 immediately generates a signal, and the first signal output terminal 55 inputs the signal to the signal receiving terminal 582 of the single-chip microcontroller 58 through the connecting line 57. After the TTL signal (Transistor Transistor Logic in English, that is, transistor-transistor logic level signal) is determined by the program, the output signal generated by the second signal output terminal 581 of the single-chip microcontroller 58 is immediately transmitted to the third-party device through the data transmission line 583. The signal can be continuously output during the time period of smoke output. Subsequently, by analyzing the start time of the signal, the start and duration of tobacco smoke exposure can be accurately determined, so as to achieve the purpose of accurately analyzing the synchronization of tobacco smoke exposure and other physiological signals of experimental animals.

[0101] Combination Figure 3 During the process of giving experimental animals tobacco smoke exposure (puffing duration 2s), each smoke signal or smoke concentration signal sensed by the smoke recorder 5 can be collected through the digital channel of the digital-to-analog converter. In the subsequent analysis of the real-time physiological information of the experimental animals, the start and end time of tobacco smoke exposure can be accurately located, which is convenient for the accurate analysis of the real-time physiological information of the experimental animals. If the passage of the smoke recorder 5 is closed, the start signal input of tobacco smoke exposure is missing during the process of collecting the real-time physiological information of the experimental animals, and the subsequent analysis cannot accurately locate the start and end time of tobacco smoke exposure, and cannot realize the analysis of the real-time impact of the tobacco smoke exposure environment on the physiological information of the experimental animals.

[0102] Although the present invention has been illustrated and described with reference to certain preferred embodiments of the present invention, it should be understood by those skilled in the art that the above is a further detailed description of the present invention in conjunction with specific embodiments, and it cannot be determined that the specific implementation of the present invention is limited to these descriptions. Those skilled in the art may make various changes in form and details, including making several simple deductions or substitutions, without departing from the spirit and scope of the present invention.

Claims

1. A method for detecting physiological information of experimental animals, characterized in that: include: Collecting real-time physiological information: collecting real-time physiological information of the experimental animals; Tobacco smoke Exposure: exposing the experimental animal to tobacco smoke during the process of collecting real-time physiological information; Collecting the time of tobacco smoke action: Collecting the time when tobacco smoke starts to act on experimental animals and the time when it ends; Information fusion: The real-time physiological information of the experimental animal is fused with the start time of the action and the end time of the action to obtain synchronous physiological information.

2. The method for detecting physiological information of experimental animals according to claim 1, characterized in that: Also includes: Collecting information on the concentration of tobacco smoke to which the experimental animal is exposed at least from the start time of the action to the end time of the action; The real-time physiological information of the experimental animal, the starting time of action, the ending time of action and the tobacco smoke concentration information are integrated to obtain concentration-synchronized physiological information.

3. The method for detecting physiological information of experimental animals according to claim 1, characterized in that: Also includes: Air at different flow rates is introduced into the tobacco smoke from the start time to the end time to adjust the concentration of the tobacco smoke exposed to the experimental animals.

4. The method for detecting physiological information of experimental animals according to claim 1, characterized in that: Use smoke detectors to detect tobacco smoke; The start time is the time when the smoke detector detects tobacco smoke, and the end time is the time when the smoke detector cannot detect tobacco smoke, or, The start time is the time when the smoke detector detects that the concentration of tobacco smoke exceeds a preset initial threshold, and the end time is the time when the smoke detector detects that the concentration of tobacco smoke is lower than a preset end threshold.

5. The method for detecting physiological information of experimental animals according to claim 4, characterized in that: The smoke detector is arranged at the mouth and nose of the experimental animal, or the smoke detector is arranged at the outlet where tobacco smoke is released, and the experimental animal is located at the outlet.

6. The method for detecting physiological information of experimental animals according to claim 1, characterized in that: The tobacco smoke exposure is performed multiple times, and the start time and the end time of the tobacco smoke exposure are collected each time.

7. The method for detecting physiological information of experimental animals according to claim 1, characterized in that: The experimental animals include macaques, rabbits or pikas, cats or dogs, mice or rats, golden hamsters, guinea pigs, gerbils or black-lined mice.

8. The method for detecting physiological information of experimental animals according to claim 1, characterized in that: The real-time physiological information includes electromyographic physiological information and / or the release level of dopamine in the brain.

9. An experimental animal physiological information detection system, used to implement the experimental animal physiological information detection method according to any one of claims 1 to 8, characterized in that: include: Tobacco smoke exposure device, used to provide an environment of tobacco smoke; An experimental chamber, used for placing the experimental animals, the experimental chamber being connected to the tobacco smoke exposure device; A physiological information collector, used to collect real-time physiological information of the experimental animal; Smoke recorder, used to record the start and end time of tobacco smoke's effect on experimental animals; The detection system is used to fuse the real-time physiological information of the experimental animal with the start time of action and the end time of action to obtain synchronous physiological information.

10. The experimental animal physiological information detection system according to claim 9, characterized in that: The smoke recorder is used to collect information on the concentration of tobacco smoke exposed to the experimental animal at least from the start time of the action to the end time of the action; The detection system is used to fuse the real-time physiological information of the experimental animal, the starting time of action, the ending time of action and the tobacco smoke concentration information to obtain concentration-synchronized physiological information.

11. The experimental animal physiological information detection system according to claim 9, characterized in that: It also includes an air conveyor, one end of which is connected to the tobacco smoke exposure device through an air conveying pipe, and the air conveyor is used to input air of different flow rates into the tobacco smoke exposure device from the start time to the end time to adjust the concentration of tobacco smoke exposed to the experimental animals.

12. The experimental animal physiological information detection system according to claim 9, characterized in that: The smoke recorder comprises: smoke detectors, which detect tobacco smoke; A single-chip microcontroller that keeps track of time.

13. The experimental animal physiological information detection system according to claim 12, characterized in that: The smoke detector is provided with a first signal output terminal for outputting tobacco smoke information detected by the smoke detector; The single-chip microcontroller is provided with a signal receiving end, a signal processing unit and a second signal output end, wherein: The signal receiving end is connected to the first signal output end, and is used to receive the tobacco smoke information output by the first signal output end; The signal processing unit is used to determine the start time and the end time of the action according to the tobacco smoke information and the time information to form smoke time information; The second signal output terminal is connected to one end of the physiological information collector, and is used to output the smoke time information to the physiological information collector; The physiological information collector is connected to the experimental animal.

14. The experimental animal physiological information detection system according to claim 13, characterized in that: It also includes a system controller, which is connected to the second signal output end and one end of the physiological information collector respectively, and is used to receive and process the smoke time information output by the second signal output end and the real-time physiological information collected by the physiological information collector.

15. The experimental animal physiological information detection system according to claim 14, characterized in that: The tobacco smoke exposure device is provided with an outlet, and the outlet is connected to the experimental chamber so that the experimental animal can be placed in the outlet.

16. The experimental animal physiological information detection system according to claim 15, characterized in that: Also includes: Smoking machines, for producing tobacco smoke; The tobacco smoke exposure device is provided with an inlet, which is connected to the smoking machine and is used to receive the tobacco smoke generated by the smoking machine.

17. The experimental animal physiological information detection system according to claim 16, characterized in that: Also includes: A ventilation hood, wherein the smoke detector is arranged inside the ventilation hood; A support member, one end of which is connected to the outlet of the tobacco smoke exposure device, and the other end of which is connected to the ventilation cover, and the ventilation cover is fixed inside the outlet through the support member.

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