External traditional Chinese medicine composition for treating eczema as well as preparation method and application of external traditional Chinese medicine composition

Abstract

The invention belongs to the field of medicines, and particularly relates to an external traditional Chinese medicine composition for treating eczema and a preparation method and application thereof. According to the external traditional Chinese medicine composition for treating eczema, the radix sophorae flavescentis, the calamine and the radix euphorbiae lantu in the formula are reused as monarch drugs, and a plurality of active ingredients with anti-inflammatory and bacteriostatic activities are added, so that the complex pathogenesis of'dampness, heat, toxicity, wind and insect 'interlaced eczema is comprehensively played, and the eczema can be cured. The traditional Chinese medicine composition has a remarkable treatment effect on clinical eczema caused by various reasons, and has a wide prospect in clinical eczema treatment.

Description

Technical Field

[0001] The invention belongs to the field of medicine, and specifically relates to an external Chinese medicine composition for treating eczema, and a preparation method and application thereof. Background Art

[0002] Eczema is a common inflammatory skin disease of the epidermis and superficial dermis caused by a variety of internal and external factors. It is a chronic disease with repeated attacks. Due to many factors such as climate and environmental changes, mental stress, accelerated pace of life, and changes in dietary structure, the incidence of eczema is on the rise. Therefore, the development of a drug that can effectively treat eczema is of great significance for the clinical prevention and treatment of eczema. Summary of the invention

[0003] The invention provides an external Chinese medicine composition for treating eczema, a preparation method and application thereof, and has a significant therapeutic effect on eczema.

[0004] The technical solution of the present invention is achieved in this way:

[0005] A Chinese medicine composition for external use for treating eczema. Each dose of the composition comprises the following components by weight: 5-15g of wolfsbane, 15-25g of schizonepeta bark, 15-25g of phellodendron, 10-20g of atractylodes, 20-40g of smilax glabra, 50-70g of sophora flavescens, 10-30g of licorice, 10-30g of stemona, 50-70g of calamine, 5-15g of alum, 5-15g of zanthoxylum bungeanum, 5-15g of lithospermum officinale, 1-2 centipedes, 5-15g of black-necked snake, 5-7g of toad skin, 5-15g of honeycomb, 10-30g of mulberry bark, 20-40g of purslane, and 10-30g of loquat leaf.

[0006] Further, the Chinese medicine composition comprises the following components by weight in each dose of the composition: 10g of wolfsbane, 20g of thorn bark, 20g of phellodendron, 15g of atractylodes, 30g of smilax, 60g of sophora flavescens, 20g of white licorice, 20g of cnidium monnieri, 15g of stemona, 60g of calamine, 10g of alum, 10g of zanthoxylum, 10g of lithospermum, 10g of centipede, 10g of black-necked snake, 6g of toad skin, 10g of beehive, 20g of mulberry bark, 30g of purslane, and 20g of loquat leaf.

[0007] The preparation method of the above-mentioned external Chinese medicine composition for treating eczema comprises the following steps:

[0008] Take the prescribed amount of wolfsbane, thorn bark, phellodendron, atractylodes, smilax, sophora flavescens, white licorice, cnidium monnieri, stemona, calamine, alum, zanthoxylum, lithospermum, centipede, black-necked snake, toad skin, beehive, mulberry bark, purslane, and loquat leaf, add water and boil to get the medicine.

[0009] Furthermore, the amount of water added is 5-10 times the total weight of the composition, and the mixture is decocted 2-3 times, each time for 20-40 minutes.

[0010] Application of the above-mentioned Chinese medicine composition in the preparation of preparations for treating eczema.

[0011] Furthermore, in the application, the prepared Chinese medicine composition is taken to wash or hot compress the affected area.

[0012] Furthermore, in the application, the dosage of the Chinese medicine composition is one dose per day.

[0013] Application of the above-mentioned Chinese medicine composition in the preparation of anti-inflammatory preparations.

[0014] The above-mentioned Chinese medicine composition is used for preparing a preparation for increasing serum interleukin-4 and / or reducing interferon-γ.

[0015] A preparation for treating eczema, comprising the above-mentioned traditional Chinese medicine composition.

[0016] Beneficial effects of the present invention:

[0017] The external Chinese medicine composition for treating eczema of the present invention is based on calamine, sophora flavescens and wolfberry chamaejasme as basic ingredients, and is added with a variety of ingredients with anti-inflammatory, antibacterial and other activities, which can dispel dampness and heat, eliminate wind toxins, kill insect evil, and tighten damp sores, and comprehensively exert multiple effects such as clearing away heat and detoxifying, cooling blood and relieving swelling, relieving itching, promoting dampness, dispelling wind, and killing insects. It has a significant therapeutic effect on eczema caused by various clinical reasons, and has broad prospects in the clinical treatment of eczema.

[0018] Sophora flavescens, calamine and wolfsbane are used as the main herbs. Sophora flavescens is bitter and cold, and has strong drying power. It can directly attack the old nest of dampness and heat, clear away the heat and toxins accumulated in the skin, and kill insects to cut off the root of itching; Calamine mainly absorbs moisture and heals sores. With its astringent nature, it can quickly stop the exudation of eczema and promote the healing of damaged areas, laying the foundation for skin repair; wolfsbane can expel water and kill insects. Expelling water will remove moisture, and killing insects will stop itching. Phellodendron chinense and Smilax glabra can purge fire and detoxify, remove dampness and benefit joints, and help Sophora flavescens to clear dampness and heat in the lower part of the body; Dictamni and Purslane can clear heat, cool blood and detoxify, and relieve the red heat caused by blood heat rash; Cnidium monnieri, Vitex turcicae, Stemona tuberosa, Zanthoxylum bungeanum, and Beehives work together to help Sophora flavescens and wolfsbane kill insects and stop itching, and eliminate the various insects that hide in the skin and induce itching. Toad skin can clear heat and dampness, fight poison with poison, and drive away the deep dampness and heat poison. Atractylodes lancea strengthens the spleen and dries dampness, eliminating the source of endogenous dampness; mulberry bark drains the lungs and promotes diuresis, guiding dampness out through urination; loquat leaves regulate the qi, and smooth qi dispels dampness and heat; lithospermum erythrorhizon cools the blood and dissolves blood stasis, while black-striped snakes and centipedes dispel wind and dredge the meridians, remove wind evil between the skin and the skin, and relieve itching and pain. The adjuvant alum draws the power of the medicine to focus on absorbing dampness and relieving itching, and harmonizes all the medicines. The whole prescription attacks evil while not forgetting to strengthen the body, dispels dampness and heat, eliminates wind poison, kills insect evil, and tightens damp sores. It addresses the complex pathogenesis of eczema, which is intertwined with "dampness, heat, poison, wind, and insects". Multiple medicines are exquisitely combined to synergistically clear heat and dampness, kill insects and relieve itching. DETAILED DESCRIPTION

[0019] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] Example 1

[0021] A Chinese medicine composition for external use for treating eczema, each dose of the composition comprising the following components by weight: 5g of wolfsbane, 15g of thorn bark, 15g of phellodendron, 10g of atractylodes, 20g of smilax, 50g of sophora flavescens, 10g of licorice root, 10g of cnidium monnieri, 10g of radix stemonae, 50g of calamine, 5g of alum, 5g of zanthoxylum bungeanum, 5g of lithospermum, 1 centipede, 5g of black-necked snake, 5g of toad skin, 5g of beehive, 10g of mulberry bark, 20g of purslane, and 10g of loquat leaf.

[0022] The preparation method of the above-mentioned external Chinese medicine composition for treating eczema comprises the following steps:

[0023] Take the formulated amount of wolfsbane, thorn bark, phellodendron, atractylodes, smilax, sophora flavescens, white mulberry bark, cnidium monnieri, stemona, calamine, alum, zanthoxylum, lithospermum, centipede, black-necked snake, toad skin, beehive, mulberry bark, purslane, and loquat leaf, add 5 times the total amount of water of the composition and boil twice, each time for 30 minutes to obtain the product.

[0024] Example 2

[0025] A Chinese medicine composition for external use for treating eczema, each dose of the composition comprising the following components by weight: 15g of wolfsbane, 25g of thorn bark, 25g of phellodendron, 20g of atractylodes, 40g of smilax, 70g of sophora flavescens, 30g of white licorice, 30g of cnidium monnieri, 20g of stemona, 70g of calamine, 15g of alum, 15g of zanthoxylum bungeanum, 15g of lithospermum, 2 centipedes, 15g of black-necked snake, 7g of toad skin, 15g of beehive, 30g of mulberry bark, 40g of purslane, and 30g of loquat leaf.

[0026] The preparation method of the above-mentioned external Chinese medicine composition for treating eczema comprises the following steps:

[0027] Take the formulated amount of wolfsbane, thorn bark, phellodendron, atractylodes, smilax, sophora flavescens, white mulberry bark, cnidium monnieri, stemona, calamine, alum, zanthoxylum, lithospermum, centipede, black-necked snake, toad skin, beehive, mulberry bark, purslane, and loquat leaf, add 8 times the total amount of water of the composition and boil for 3 times, each time for 20 minutes to obtain the product.

[0028] Example 3

[0029] Further, the Chinese medicine composition comprises the following components by weight in each dose of the composition: 10g of wolfsbane, 20g of thorn bark, 20g of phellodendron, 15g of atractylodes, 30g of smilax, 60g of sophora flavescens, 20g of white licorice, 20g of cnidium monnieri, 15g of stemona, 60g of calamine, 10g of alum, 10g of zanthoxylum, 10g of lithospermum, 10g of centipede, 10g of black-necked snake, 6g of toad skin, 10g of beehive, 20g of mulberry bark, 30g of purslane, and 20g of loquat leaf.

[0030] The preparation method of the above-mentioned external Chinese medicine composition for treating eczema comprises the following steps:

[0031] Take the formulated amount of wolfsbane, thorn bark, phellodendron, atractylodes, smilax, sophora flavescens, white mulberry bark, cnidium monnieri, stemona, calamine, alum, zanthoxylum, lithospermum, centipede, black-necked snake, toad skin, beehive, mulberry bark, purslane, and loquat leaf, add 10 times the total amount of water of the composition and boil for 3 times, each time for 30 minutes to obtain the product.

[0032] The Chinese medicine composition prepared in the above embodiment is used to treat eczema. The prepared water decoction is taken to wash the affected area, one dose per day. It has a significant therapeutic effect on eczema caused by various reasons, is not prone to recurrence, and has a significant therapeutic effect.

[0033] 1. In vitro efficacy study

[0034] Experimental animals: SPF grade 6-week-old mice were selected, half male and half female, weighing 20-25g. The animals were kept in the Animal Experiment Center of Hainan Medical College, and all procedures and animal experiments were approved by the Ethics Committee of Hainan Medical College. The animals were kept at 20-25℃ and 40±5% humidity, and food and water were available ad libitum.

[0035] All mice were fed adaptively under feeding conditions for 1 week before modeling. 0.1g DNCB was dissolved in 10mL acetone-olive oil solution (volume ratio 4:1) to prepare 1% DNCB acetone solution, and 0.5g DNCB was dissolved in 10mL acetone-olive oil solution (volume ratio 4:1) to prepare 5% DNCB acetone solution. After preparation, put it in the refrigerator for use.

[0036] One day before the experiment, scissors and depilatory cream were used to remove 2cmx2cm area of ​​mouse hair on the back to expose the skin. Except for the blank group, the other experimental groups were treated as follows: from the first day of the experiment, 50μL of 5% DNCB acetone solution was applied on the back of the mice once a day for 3 days, and depilation was performed again on the 5th day. From the 6th day, 50μL of 1% DNCB acetone solution was applied on the back of the mice once a day for 5 days. 24 hours after application, typical acute eczema manifestations such as redness, swelling, papules, exudation, and scabs appeared on the back skin, proving that the model was successfully established.

[0037] The original prescription groups 1-3 were given the Chinese medicine compositions prepared in Examples 1-3, and the reduced prescription groups were given the Chinese medicine compositions prepared in reduced prescriptions 1-3, respectively. The medicinal material compositions of each reduced prescription group are as follows, and the preparation method is referred to Example 3. The model group and the blank group were not treated in any way.

[0038] Reduced prescription 1: Medicinal ingredients: Phellodendron 20g, Atractylodes 15g, Smilax glabra 30g, Sophora flavescens 60g, Dictamni 20g, Cnidium monnieri 20g, Stemona 15g, Zanthoxylum bungeanum 10g, Portulaca oleracea 30g.

[0039] Ingredients of reduced prescription 2: 10g of wolfsbane, 20g of thorn bark, 60g of calamine, 10g of alum, 10g of lithospermum, 1 centipede, 10g of black-necked snake, 6g of toad skin, 10g of beehive, 20g of mulberry bark, and 20g of loquat leaf.

[0040] Ingredients of reduced prescription 3: Phellodendron 20g, Atractylodes 15g, Smilax glabra 30g, Sophora flavescens 60g, Dictamni 20g, Cnidium monnieri 20g, Stemona 15g, Zanthoxylum bungeanum 10g, Portulaca oleracea 30g, Calamine 60g, Alum 10g.

[0041] The administration method is as follows: the mouse's limbs are fixed, and 3 layers of gauze are covered on the exposed skin of the mouse. Each sample to be tested is drawn with a syringe and dropped onto the gauze until the gauze is filled with the drug solution and no more drops are dripped out. After 10 minutes, new drug solution is added again, and a total of 3 drug addition treatments are performed. The drug treatment is performed once in the morning and afternoon every day. The blank control group does not receive any treatment. After 7 consecutive days of treatment, peripheral blood of the mice is taken, centrifuged at 4°C and 3000 rpm for 15 minutes, and the supernatant is collected. The serum interleukin 4 (IL-4) and gamma interferon (IFN-γ) levels are detected according to the instructions of the kit, and the mouse serum IL-4 and IFN-γ levels are detected. The results are shown in Table 1.

[0042] Table 1 Comparison of IL-4 and IFN-γ contents in Chinese medicine compositions with different formulations

[0043] Group IL-4 (ng / L) IFN-γ(ng / L) Blank group 61.56 75.43 Model Group 145.56 47.67 Original group 1 90.35 71.37 Original group 2 91.66 71.53 Original group 3 87.23 70.65 1 group of reduction 126.58 55.27 Reduce 2 groups 130.64 51.83 3 groups of reduction 110.37 60.34

[0044] As can be seen from Table 1, the three embodiment groups of the present invention can significantly reduce the IL-4 content in mouse serum and increase the IFN-γ content in mouse serum. The effect is not only significantly higher than that of the model group, but also has different degrees of improvement relative to the three reduced prescription groups. It shows that the three embodiment groups of the present invention can produce an additive effect through the combination of medicinal materials, and can significantly improve the anti-inflammatory effect of the entire prescription. At the same time, during the experiment, no adverse reactions such as poisoning and allergies occurred in the animals of the original prescription group and the reduced prescription group, indicating that the Chinese medicine composition described in the present invention has less side effects and is safe to use.

[0045] 2. Clinical efficacy study

[0046] Using the "Guidelines for Clinical Research of New Chinese Medicines (Trial)" as the diagnostic criteria, 120 patients with eczema who visited the First Affiliated Hospital of Hainan Medical College were included in the study and randomly divided into 3 implementation groups and a control group (Western medicine treatment), with 30 cases in each group. There was no statistically significant difference in gender, age, and course of disease between the two groups (P>0.05), and they were comparable. This study was approved by the Ethics Committee of Hainan Medical College, and the patients and their families signed the informed consent.

[0047] The embodiment group adopts the Chinese medicine composition prepared by embodiment 1-3 to treat respectively, one dose every 3 days, takes water decoction, washes the affected part, washes once in the morning and evening. If it is for the hands, feet and other parts, the hot liquid is soaked, 30min each time. If it is for other parts that are not convenient to soak, such as chest, abdomen, face, etc., the towel is soaked in the liquid for hot compress to administer, and the hot compress time is 30min each time. During the hot compress process, if the liquid cools, the hot liquid is re-added for hot compress. The control group selects calamine lotion (no exudation) or zinc oxide ointment (with exudation) for treatment according to the condition. A course of treatment of 7 days, the longest treatment of 3 courses, statistical treatment effect. The recurrence situation was visited after the treatment was completed for 1 month.

[0048] Table 2 Clinical efficacy of the Chinese medicine composition of the present invention

[0049]

[0050] As can be seen from Table 2, the Chinese medicine composition prepared by the three embodiment groups of the present invention is used for the treatment of clinical eczema, and the total effective rate can reach more than 90%, which is significantly improved relative to the control group, and the cure rate can reach more than 43.33%, which is also significantly higher than the control group. And between the three embodiment groups, the total effective rate and the cure rate are not significantly different, indicating that the Chinese medicine composition of the present invention has a stable efficacy and has a significant therapeutic effect on eczema of various causes. After the treatment, the cured patients were revisited, and only one patient in the embodiment 1 group relapsed, and the relapse rate among the cured patients was 6.67%. In the control group, 4 patients relapsed among the cured patients, and the relapse rate was 40%. It shows that the use of the Chinese medicine composition of the present invention to treat eczema can significantly reduce the relapse rate of patients after cure, and has a significant preventive effect on the recurrence of eczema.

[0051] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A Chinese medicine composition for external use for treating eczema, characterized in that: Each dose of the composition includes the following components by weight: 5-15g of wolfsbane, 15-25g of thorn bark, 15-25g of phellodendron, 10-20g of atractylodes, 20-40g of smilax glabra, 50-70g of sophora flavescens, 10-30g of white licorice, 10-30g of cnidium monnieri, 10-20g of stemona, 50-70g of calamine, 5-15g of alum, 5-15g of zanthoxylum bungeanum, 5-15g of lithospermum, 1-2 centipedes, 5-15g of black-necked snake, 5-7g of toad skin, 5-15g of beehive, 10-30g of mulberry bark, 20-40g of purslane, and 10-30g of loquat leaf.

2. A Chinese medicine composition for external use for treating eczema according to claim 1, characterized in that: The Chinese medicine composition comprises the following components by weight in each dose of the composition: 10g of wolfsbane, 20g of thorn bark, 20g of phellodendron, 15g of atractylodes, 30g of smilax, 60g of sophora flavescens, 20g of white licorice, 20g of cnidium monnieri, 15g of stemona, 60g of calamine, 10g of alum, 10g of zanthoxylum bungeanum, 10g of lithospermum, 1 centipede, 10g of black-necked snake, 6g of toad skin, 10g of beehive, 20g of mulberry bark, 30g of purslane, and 20g of loquat leaf.

3. A method for preparing a Chinese medicine composition for external use for treating eczema according to claim 1 or 2, characterized in that: The following steps are involved: Take the prescribed amount of wolfsbane, thorn bark, phellodendron, atractylodes, smilax, sophora flavescens, white licorice, cnidium monnieri, stemona, calamine, alum, zanthoxylum, lithospermum, centipede, black-necked snake, toad skin, beehive, mulberry bark, purslane, and loquat leaf, add water and boil to get the medicine.

4. The method for preparing a Chinese medicine composition for external use for treating eczema according to claim 3, characterized in that: The amount of water added is 5-8 times the total weight of the composition, and the mixture is decocted 2-3 times, each time for 20-40 minutes.

5. Use of the external Chinese medicine composition for treating eczema as claimed in claim 1 or 2 in the preparation of a preparation for treating eczema.

6. The use according to claim 5, characterized in that In the application, the prepared Chinese medicine composition is taken to wash the affected area.

7. The use according to claim 5, characterized in that In the application, the dosage of the Chinese medicine composition is one dose per day.

8. Use of the external Chinese medicine composition for treating eczema as claimed in claim 1 or 2 in the preparation of anti-inflammatory preparations.

9. Use of the external Chinese medicinal composition for treating eczema as claimed in claim 1 or 2 in the preparation of a preparation for increasing serum interleukin-4 and / or reducing interferon-γ.

10. A preparation for treating eczema, characterized in that: A Chinese medicine composition comprising the Chinese medicine composition according to claim 1 or 2.

Images