Compositions, methods, and uses for sows in synchronizing farrowing

Abstract

The application provides a composition, a method and application thereof for sows to be delivered at the same time, and particularly discloses a composition and a method for sows to be delivered at the same time by using vitamin B6, mifepristone, combined with sodium cloprostenol and carbetocin to shorten the delivery process and improve the live birth rate. The composition of the application aims at the problem that the live birth rate is reduced due to the sows to be delivered at the same time, thereby affecting the production efficiency of sows, and on the basis of the delivery drugs sodium cloprostenol and carbetocin, vitamin B6 and mifepristone are combined to form a new delivery at the same time scheme. The application shortens the delivery process of sows and improves the live birth rate of sows, and is a method for improving the delivery effect of batch sows at the same time, which provides great value for improving the PSY of sows and promoting the batch management of sows.

Description

TECHNICAL FIELD

[0001] The present application mainly relates to the field of animal reproduction regulation, and particularly relates to a method for improving the effect of farrowing synchronization in batch production of sows, and particularly relates to a composition for farrowing synchronization of sows, a method and application thereof. BACKGROUND

[0002] The batch production technology of sows is to divide the sow group according to the scale of the sow group and the production plan, supplement the replacement sows according to the production plan, and use biological technology to achieve the purpose of synchronization of estrus, synchronization of breeding and synchronization of farrowing of the replacement sows and the sows in the same batch, which is a high-efficiency controllable management technical system for improving the reproductive performance of the sow group in the pig farm. Among them, the farrowing synchronization of sows is one of the key links of the batch production of sows. After the sow initiates labor, the uterus contracts rhythmically. The longer the time for the born piglets to stay in the sow's uterus or birth canal, the higher the risk of umbilical cord rupture, and the greater the risk of piglet death. Especially in the later stage of sow labor, the labor force is insufficient, the uterine contraction is weak, the umbilical cord of the piglet cannot be timely delivered, and the risk of asphyxiation death increases. In addition, even if the sows are synchronized breeding, due to individual differences of sows, the farrowing initiation time of sows in the same batch is inconsistent, and the farrowing time span of the whole batch of sows is about 5-7 days, which leads to great difficulty in batch management and seriously affects the production efficiency of batch production of sows. With the increase in the number of high-yield sows, the problem of increasing number of dead fetuses and mummies due to long labor is becoming more and more obvious, therefore, establishing an efficient farrowing synchronization technology of sows is the key to guaranteeing the efficiency of batch production and realizing precise control.

[0003] The existing farrowing synchronization technology often uses the method of sodium cloprostenol, sodium cloprostenol combined with carbetocin or oxytocin to realize the farrowing synchronization of sows. However, the use of sodium cloprostenol alone still has a large span of farrowing time of batch sows; the combined use of oxytocin can induce the sows to farrow in a concentrated manner, but it can easily lead to prolonged labor and increased number of dead fetuses; and the combined use of carbetocin can improve the concentration of sow farrowing and shorten the labor process, but the number of live births and the live piglet rate are both decreased. In view of the above reasons, the pig breeding industry urgently needs a simple and efficient batch production sow farrowing synchronization technology to replace the original sow farrowing synchronization scheme.

[0004] Vitamin B6 is a water-soluble B vitamin, which exists in the form of pyridoxine, pyridoxal, pyridoxamine, pyridoxine phosphate, pyridoxal phosphate and pyridoxamine phosphate in the body, participates in key biochemical processes such as amino acid metabolism and neurotransmitter synthesis, has physiological functions such as anti-inflammatory, antioxidant, nerve regulation and antitumor, and is essential for maintaining the health of the nervous system, the health of the skin and immune function.

[0005] Mifepristone is an organic compound with the chemical formula C 29 H 35NO2, a new antiprogestin with anti-glucocorticoid activity, is used as an anticonception drug. It has 5 times stronger affinity to progesterone receptor than progesterone. It is used for anticonception, inducing menstruation, inducing labor of intrauterine death, etc.

[0006] However, up to now, there is no report or suggestion that vitamin B6 and mifepristone are used for shortening the delivery process and increasing the live birth rate of multiple animals. SUMMARY

[0007] Through a large number of creative experiments, the present application compares the effects of combined administration of vitamin B6, mifepristone, sodium cloprostenol and carbetocin on synchronous delivery, and finds that the combined administration of vitamin B6, mifepristone, sodium cloprostenol and carbetocin can effectively shorten the delivery process of batch sow synchronous delivery and increase the live birth rate, thereby establishing a method for improving batch sow synchronous delivery and providing important value for the popularization and application of batch sow production. Specifically, the present application includes the following contents.

[0008] In a first aspect, the present application provides a composition for sow synchronous delivery, which comprises vitamin B6, mifepristone, sodium cloprostenol and carbetocin.

[0009] In certain embodiments, the composition for sow synchronous delivery according to the present application, wherein the composition comprises 0.2-20 g of vitamin B6, 50-1000 mg of mifepristone, 0.3-3 mg of sodium cloprostenol and 20-100 μg of carbetocin.

[0010] In a second aspect, the present application provides a method for improving sow synchronous delivery, which comprises the step of administering the composition of the first aspect to sows.

[0011] In certain embodiments, the method for improving sow synchronous delivery according to the present application, wherein the method comprises the step of administering vitamin B6, mifepristone, sodium cloprostenol and carbetocin to sows at a suitable gestational day after synchronous breeding.

[0012] In certain embodiments, the method for improving sow synchronous delivery according to the present application, wherein the method comprises the following steps:

[0013] (1) Synchronous breeding of sows, followed by pregnancy examination to confirm pregnancy;

[0014] (2) Administration of vitamin B6 to sows at 100-115 days of pregnancy;

[0015] (3) Administration of mifepristone to sows at 112-115 days of pregnancy;

[0016] (4) The sows are administered with dinoprostone sodium at 113-115 days of pregnancy;

[0017] (5) The sows are administered with carbetocin at 113-115 days of pregnancy.

[0018] In some embodiments, the method for improving the farrowing of sows in batches according to the present application, wherein the vitamin B6 is administered at 0.2-20 g per day, the administration time of vitamin B6 is at 100-113 days of pregnancy, the administration is once a day, and the administration mode is oral or injection.

[0019] In some embodiments, the method for improving the farrowing of sows in batches according to the present application, wherein the mifepristone is administered at 50-1000 mg per time, the administration time of mifepristone is at 112-115 days of pregnancy, and the administration mode is oral or injection.

[0020] In a third aspect, the present application provides the use of the composition or the method according to the present application in the batch production of sows.

[0021] In some embodiments, the use according to the present application, wherein the use comprises: shortening the farrowing of sows, improving the live birth rate of sows.

[0022] The method of the present application is based on the batch farrowing scheme of sows by combined administration of vitamin B6, mifepristone, dinoprostone sodium and carbetocin, which greatly shortens the farrowing of sows and improves the live birth rate of sows. The method of the present application can effectively alleviate the production problem of the decrease of the live birth rate of sows in the batch production of sows, and has great value for improving the PSY (the number of weaned piglets per sow per year) of sows and promoting the batch production of sows. BRIEF DESCRIPTION OF DRAWINGS

[0023] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0024] Figure 1 The batch farrowing flowchart of sows based on combined administration of vitamin B6, mifepristone, dinoprostone sodium and carbetocin. DETAILED DESCRIPTION

[0025] The various exemplary embodiments of the present application will now be described in detail, which should not be considered as limiting the present application, but should be understood as a more detailed description of certain aspects, characteristics and embodiments of the present application.

[0026] It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the present application. Additionally, for a range of values of a parameter, unless otherwise stated, the inclusion of either extreme or any other value within the ranges is expressly intended. Excluded from the description are any intermediate values or intermediate ranges that do not qualify as the lower or upper limit of a range.

[0027] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present application, the preferred methods and materials are described. All patents, patent applications, publications, and descriptions mentioned herein are incorporated by reference to the extent allowed by law for disclosure and description of methods and / or materials related to the subject matter of the present application. In the case of conflict between the description contained herein and that of any such incorporated literature, the present description controls.

[0028] Composition for synchronizing farrowing of sows

[0029] In one aspect of the present application, there is provided a composition for improving the synchronization of farrowing in sows, in particular for improving the synchronization of farrowing in batch production of sows, comprising or consisting of: vitamin B6, mifepristone, cloprostenol sodium and carbetocin.

[0030] In a preferred embodiment, the composition of the present application comprises 0.2-20 g (preferably 0.2-10 g, and further preferably 0.2-9 g, such as 0.2-8 g, 0.2-7 g, 0.2-6 g, 0.2-5 g, 0.2-4 g, 0.2-3 g, 0.2-2 g, 0.3-2 g, 0.4-2 g, more preferably 0.5-2 g, such as 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0 g, or any range within the aforementioned amounts) of vitamin B6; 50-1000 mg (preferably 100-500 mg, and further preferably 100-400 mg, such as 100, 150, 200, 250, 300, 350, 400 mg, or any range within the aforementioned amounts) of mifepristone. The amounts of cloprostenol sodium and carbetocin can be used in the amounts conventionally used in the art for farrowing of sows, which are not particularly limited.

[0031] Method for improving synchronization of farrowing of sows

[0032] One aspect of the present invention provides a method for improving sow simultaneous farrowing, particularly for improving sow simultaneous farrowing in batch production, comprising the step of administering the composition described in the present invention to the sow.

[0033] In one preferred embodiment, the method for simultaneous farrowing in batch-producing sows using vitamin B6 and mifepristone combined with cloprostenol sodium and carbetocin involves feeding sows vitamin B6 during gestation 100-115 days, injecting mifepristone during gestation 112-115 days, injecting cloprostenol sodium during gestation 113-115 days, and injecting carbetocin during gestation 113-115 days. In another preferred embodiment, the method for simultaneous farrowing in batch-producing sows using vitamin B6 and mifepristone combined with cloprostenol sodium and carbetocin involves feeding sows vitamin B6 during gestation 100-113 days, injecting mifepristone during gestation 112-115 days, injecting cloprostenol sodium during gestation 113-115 days, and injecting carbetocin during gestation 113-115 days. In another preferred embodiment, the method for batch production of sows with vitamin B6, mifepristone, cloprostenol sodium, and carbetocin for simultaneous farrowing is as follows: sows after simultaneous mating are fed vitamin B6 once a day during gestation days 100-113, injected with mifepristone on gestation day 112, injected with cloprostenol sodium on gestation day 113, and injected with carbetocin on gestation day 114.

[0034] In a preferred embodiment, a batch production method for sows to produce sows with synchronized farrowing using vitamin B6, mifepristone, cloprostenol sodium, and carbetocin includes the following steps:

[0035] (1) Sows are bred at the same time, then pregnancy is checked to confirm pregnancy, and then they are transferred to the farrowing house;

[0036] (2) Administer vitamin B6 to sows during 100-115 days of gestation;

[0037] (3) Mifepristone was administered to sows during 112-115 days of gestation;

[0038] (4) Administer sodium cloprostenol to sows during 113-115 days of gestation;

[0039] (5) Administer carbetocin to sows during 113-115 days of gestation.

[0040] In another preferred embodiment, a batch production method for sows to produce sows simultaneously using vitamin B6, mifepristone, cloprostenol sodium, and carbetocin includes the following steps:

[0041] (1) Sows are bred at the same time, then pregnancy is checked to confirm pregnancy, and then they are transferred to the farrowing house;

[0042] (2) The sows are fed with vitamin B6 at 100-113 days of pregnancy, once a day;

[0043] (3) The sows are injected with mifepristone at 112 days of pregnancy;

[0044] (4) The sows are injected with sodium cloprostenol at 113 days of pregnancy;

[0045] (5) The sows are administered carbetocin at 114 days of pregnancy.

[0046] In a preferred embodiment of the method for improving the farrowing of sows, the amount of vitamin B6 is 0.2-20 g / day, and it is also preferred to be 0.2-15 g / day, further preferred to be 0.2-10 g / day, more preferred to be 0.5-10 g / day, for example 0.5-5 g / day, 0.5-4 g / day, 0.5-3 g / day, and most preferred to be 0.5-2 g / day, for example 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0 g, or any range within the above-mentioned amount. The administration of vitamin B6 is oral or injection, and it is preferred to be oral.

[0047] In a preferred embodiment of the method for improving the farrowing of sows, the amount of mifepristone is 50-1000 mg in a single administration, preferably 100-500 mg, and more preferably 100-400 mg, for example 100, 150, 200, 250, 300, 350, 400 mg, or any range within the above-mentioned amount. The administration of mifepristone is oral or injection, and it is preferred to be injection.

[0048] Use

[0049] In one aspect of the present application, the application of the composition or the method described in the present application in batch production of sows is also provided. The present application provides a method for the farrowing of sows by using vitamin B6, mifepristone, sodium cloprostenol and carbetocin, so as to shorten the delivery process of sows and improve the live birth rate of sows; vitamin B6, mifepristone, sodium cloprostenol and carbetocin can alleviate the production problem of the decrease of the live birth rate of sows in batch production.

[0050] Examples

[0051] The following shows the application of vitamin B6, mifepristone, sodium cloprostenol and carbetocin in the farrowing of sows in batch production.

[0052] (1) Experimental method and grouping

[0053] The batch production of white sow is randomly divided into (a)-(f) six groups, chloroprostol sodium group + carbetocin group, vitamin B6 + chloroprostol sodium group + carbetocin group, mifepristone + chloroprostol sodium + carbetocin group, vitamin B6 (low dose group) + mifepristone + chloroprostol sodium + carbetocin group, vitamin B6 (medium dose group) + mifepristone + chloroprostol sodium + carbetocin group, vitamin B6 (high dose group) + mifepristone + chloroprostol sodium + carbetocin group, the body weight of sow is 250-270 kg, the amount of daily ration is 2.5-3 kg. The experimental sow in each group is fed according to the requirements of the field feeding management during the experiment, and the water is free. The daily ration can use the formula used during the pregnancy of sow (such as the formula recommended in “Sow Batch Production Management Technology”) or adjust the components according to the needs. The daily ration used in this embodiment is shown in Table 1.

[0054] Table 1 Nutritional level of daily ration for sow during pregnancy

[0055]

[0056] The specific grouping is as follows:

[0057] (a) Chloroprostol sodium group + carbetocin group: 2 ml (per sow) of chloroprostol sodium injection is injected into the sow at 9:00 am on the 113th day of pregnancy, and 1 ml (per sow) of carbetocin injection is injected into the sow at 9:00 am on the 114th day of pregnancy.

[0058] (b) Vitamin B6 + chloroprostol sodium group + carbetocin group: 2 g (per sow) of vitamin B6 is fed to the sow at 8:00 am every day until the sow reaches the 113th day of pregnancy, 2 ml (per sow) of chloroprostol sodium injection is injected into the sow at 9:00 am on the 113th day of pregnancy, and 1 ml (per sow) of carbetocin injection is injected into the sow at 9:00 am on the 114th day of pregnancy.

[0059] (c) Mifepristone + chloroprostol sodium + carbetocin group: 400 mg (per sow) of mifepristone is injected into the sow at 17:00 pm on the 112th day of pregnancy, 2 ml (per sow) of chloroprostol sodium injection is injected into the sow at 9:00 am on the 113th day of pregnancy, and 1 ml (per sow) of carbetocin injection is injected into the sow at 9:00 am on the 114th day of pregnancy.

[0060] (d) Vitamin B6 (low dose group) + mifepristone + sodium cloprostenol + carbetocin group: 0.1 g (per pig) of vitamin B6 was fed to the sows every day from 8:00 am on the 108th day of pregnancy until the expected delivery date of the sows on the 113th day of pregnancy, 200 mg (per pig) of mifepristone was injected into the sows via the back of the ear at 17:00 pm on the 112th day of pregnancy, 2 ml (per pig) of sodium cloprostenol injection was injected into the sows via the back of the ear at 9:00 am on the 113th day of pregnancy, and 1 ml (per pig) of carbetocin injection was injected into the sows at 9:00 am on the 114th day of pregnancy.

[0061] (e) Vitamin B6 (medium dose group) + mifepristone + sodium cloprostenol + carbetocin group: 1.0 g (per pig) of vitamin B6 was fed to the sows every day from 8:00 am on the 108th day of pregnancy until the expected delivery date of the sows on the 113th day of pregnancy, 200 mg (per pig) of mifepristone was injected into the sows via the back of the ear at 17:00 pm on the 112th day of pregnancy, 2 ml (per pig) of sodium cloprostenol injection was injected into the sows via the back of the ear at 9:00 am on the 113th day of pregnancy, and 1 ml (per pig) of carbetocin injection was injected into the sows at 9:00 am on the 114th day of pregnancy.

[0062] (f) Vitamin B6 (high dose group) + mifepristone + sodium cloprostenol + carbetocin group: 10.0 g (per pig) of vitamin B6 was fed to the sows every day from 8:00 am on the 108th day of pregnancy until the expected delivery date of the sows on the 113th day of pregnancy, 200 mg (per pig) of mifepristone was injected into the sows via the back of the ear at 17:00 pm on the 112th day of pregnancy, 2 ml (per pig) of sodium cloprostenol injection was injected into the sows via the back of the ear at 9:00 am on the 113th day of pregnancy, and 1 ml (per pig) of carbetocin injection was injected into the sows at 9:00 am on the 114th day of pregnancy.

[0063] (2) Observation and recording of the delivery of sows

[0064] The delivery date, time, duration, and birth of the sows (including the total number of births, the number of live births, the number of stillbirths, and the number of mummies, the average delivery duration, and the live birth rate were recorded.

[0065] (3) Statistical analysis

[0066] Single-factor variance analysis and chi-square test were performed using SPSS 22.0 software (SPSS GmbH, Munich, Germany). Single-factor variance analysis was used to analyze the average delivery duration, the average number of births per litter, the average number of live births per litter, the average number of stillbirths per litter, and the average number of mummies per litter, and the results were expressed as mean ± standard deviation. Chi-square test was used to analyze the live birth rate of sows. P<0.05 was considered statistically significant.

[0067] (4) Experimental results

[0068] In the present embodiment, compared with the control group (prostaglandin F2a sodium + carbetocin group), the vitamin B6 + prostaglandin F2a sodium + carbetocin group, the mifepristone + prostaglandin F2a sodium + carbetocin group can significantly shorten the delivery process, significantly reduce the number of dead fetuses, and significantly improve the live birth rate; in the three groups of vitamin B6 + mifepristone + prostaglandin F2a sodium + carbetocin, when the dose of vitamin B6 reaches the medium / high dose, the delivery process of the sow is further shortened, the number of live births is increased, the number of dead fetuses is reduced, and the live birth rate is significantly higher than the above three groups. However, when the dose of vitamin B6 is low, even if it is combined with mifepristone, prostaglandin F2a sodium and carbetocin, compared with the vitamin B6 + prostaglandin F2a sodium + carbetocin group and the mifepristone + prostaglandin F2a sodium + carbetocin group, there is no significant difference in the live birth rate (Table 2).

[0069] Table 2 Effect of different synchronized delivery schemes on the delivery process and litter of sows

[0070]

[0071] Note: Different lowercase letters marked on the right shoulder indicate that the indicators in the same row are significantly different, P<0.05 .

[0072] Industrial value of the present application:

[0073] Since the first outbreak of African swine fever in August 2018, the traditional continuous production mode has been difficult to adapt to the rapid development of the pig industry, and the entire pig market is facing severe challenges, so batch production technology of sows has become an important measure for transformation and upgrading. In the future, the era of small-scale pig raising will become a thing of the past, and the emergence of large-scale and batch production methods will certainly bring about earth-shaking changes to the pig industry. However, the low live birth rate caused by synchronized delivery of sows seriously affects the efficiency of the pig farm. However, the present application is a method for improving the effect of synchronized delivery of sows based on the combination of vitamin B6, mifepristone, prostaglandin F2a sodium and carbetocin (the method for improving the effect of synchronized delivery of sows is established based on the combination of vitamin B6, mifepristone, prostaglandin F2a sodium and carbetocin), which provides important value for the popularization and application of batch production of sows. The present application effectively improves the live birth rate of batch sows. Figure 1

[0074] The batch synchronized delivery technology of sows established by the present application through the combination of vitamin B6, mifepristone, prostaglandin F2a sodium and carbetocin makes the number of dead fetuses of batch delivery sows decrease by 0.59, effectively increases the number of live births per litter by 0.71, and according to the calculation of 2.23 litters per sow per year and 95% survival rate of weaned piglets in China in 2024, it will make the PSY increase by 0.71*2.23*95%=1.50, so the present application is an effective way to improve the quality and efficiency of pig breeding, and the present application also has the advantages of simplicity, high efficiency, strong innovation, broad industrial prospects and value.

[0075] ​Although the embodiments of the present application have been shown and described above, it is understood that the above-described embodiments are exemplary and are not to be construed as limiting the present application, and that variations, modifications, substitutions and changes can be made by those skilled in the art without departing from the scope of the present application.

Claims

1. The use of vitamin B6, mifepristone, cloprostenol sodium and carbetocin in combination for preparing a medicine for improving the farrowing in synchrony of sows, characterized in that, The improved farrowing of sows is to shorten the farrowing process of sows and / or improve the live piglet rate, and the improved farrowing of sows comprises the following steps: (1) Sows are synchronized with mating, and then pregnancy examination is performed to confirm pregnancy; (2) Vitamin B6 is administered to sows every day when the sows are 108-113 days pregnant; (3) Mifepristone is administered to sows when the sows are 112 days pregnant; (4) Sodium prostaglandin F2a is administered to sows when the sows are 113 days pregnant; (5) Carbetocin is administered to sows when the sows are 114 days pregnant; The dosage of vitamin B6 is 1.0-10 g per day, once a day, the dosage of mifepristone is 100-400 mg for single administration, and the dosages of sodium prostaglandin F2a and carbetocin for single administration are the conventional dosages for sows to deliver.

2. Use according to claim 1, characterized in that, The administration modes of vitamin B6, mifepristone, sodium prostaglandin F2a and carbetocin are oral or injection.

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