Wearable multi-functional plant growth monitoring device and system

Abstract

The present invention provides a wearable multi-element collaborative plant growth monitoring device and system, which relates to the field of plant cultivation technology. The device includes: a gas sensor module for detecting gas information in the plant's surrounding environment, a laser sensor module for detecting the diameter of the plant stem, an electrochemical sensor module for collecting the juice of the target plant and obtaining the biological information of the target plant based on the juice, and a central control module for coordinating and controlling the actions of multiple modules; the diameter of the stem of the target plant is obtained through the laser sensor module, and the biological information is collected through the electrochemical sensor module after the gas information and / or the stem diameter reaches the preset judgment condition, so that multiple modules can work together, focus on key detection nodes, improve the accuracy of plant growth monitoring and the effectiveness of detection results, improve the accuracy and efficiency of planting in agricultural work, and achieve improvements such as increased production, improved quality, and energy saving.

Description

Technical Field

[0001] The present invention relates to the field of plant cultivation technology, and in particular to a wearable multi-element collaborative plant growth monitoring device and system. Background Art

[0002] With the development of agricultural technology, real-time monitoring of the microbial environment of plant growth has become increasingly important.

[0003] Plant growth environment monitoring can keep an eye on plant growth at any time. The main detection items generally include: environmental gas monitoring, stem diameter monitoring and fruit bioinformation monitoring. Traditional plant growth monitoring modules are all carried out independently, requiring a lot of manpower intervention, which is time-consuming and labor-intensive, and often misses key detection time nodes, affecting the accuracy of plant growth monitoring and the effectiveness of test results. Summary of the invention

[0004] The purpose of the present invention is to provide a wearable multi-element collaborative plant growth monitoring device and system, which obtains gas information of the surrounding environment of the target plant through a gas sensor module, obtains the stem diameter of the target plant through a laser sensor module, and collects biological information through an electrochemical sensor module after the gas information and / or the stem diameter reaches a preset judgment condition, so that multiple modules can work together and focus on key detection nodes, thereby improving the accuracy of plant growth monitoring and the effectiveness of detection results, improving the accuracy and efficiency of planting in agricultural work, and achieving improvements such as increased production, improved quality, and energy conservation.

[0005] In a first aspect, the present invention provides a wearable multi-element collaborative plant growth monitoring device, comprising: a gas sensor module, a laser sensor module, an electrochemical sensor module and a central control module;

[0006] A gas sensing module, used to detect gas information in the surrounding environment of the target plant and send the gas information to the central control module; wherein the gas information includes at least one of the following: ethylene gas concentration, carbon dioxide gas concentration or nitric oxide gas concentration;

[0007] A laser sensor module is used to obtain the stem diameter of the target plant and send the stem diameter of the target plant to the central control module;

[0008] The central control module is used to generate an action signal if the gas information meets the preset conditions and / or the diameter of the stem of the target plant meets the preset growth conditions, and send the action signal to the electrochemical sensor module;

[0009] The electrochemical sensor module is used to receive action signals, collect juice from the target plant, obtain biological information of the target plant based on the juice, and send the biological information to the central control module; wherein the biological information includes at least one of the following: sugar content, acidity or vitamin C content.

[0010] In some preferred embodiments of the present invention, the gas sensing module includes: a flexible gas substrate, a gas signal transmission processing unit and at least one gas sensor;

[0011] The gas signal transmission processing unit and the gas sensor are arranged on the flexible gas substrate;

[0012] A gas sensor, used for generating a gas electrical signal based on the concentration of the target gas, and sending the gas electrical signal to a gas signal transmission processing unit;

[0013] The gas signal transmission processing unit is used to amplify the gas electrical signal and transmit it to the central control module.

[0014] In some preferred embodiments of the present invention, the gas sensor includes at least one of the following: a flexible ethylene gas sensor, a flexible carbon dioxide gas sensor or a flexible nitric oxide gas sensor.

[0015] In some preferred embodiments of the present invention, the laser sensor module includes: a laser transmitter and a laser receiver arranged opposite to each other and a laser data processing unit;

[0016] a laser transmitter for emitting a laser beam; a portion of the laser beam being blocked by a stem of a target plant;

[0017] A laser receiver, used for receiving a laser beam;

[0018] The laser data processing unit is used to determine the diameter of the stem of the target plant based on the laser beam received by the laser receiver.

[0019] In some preferred embodiments of the present invention, the laser sensor module further comprises: a first position adjustment mechanism and a second position adjustment mechanism;

[0020] The laser emitter is arranged on the first position adjustment mechanism;

[0021] The laser receiver is arranged on the second position adjustment mechanism;

[0022] a first position adjustment mechanism, for adjusting the position of the laser emitter relative to the target plant;

[0023] The second position adjustment mechanism is used to adjust the position of the laser receiver relative to the target plant.

[0024] In some preferred embodiments of the present invention, the electrochemical sensing module includes: an electrode substrate, a microneedle, a working electrode and a signal converter;

[0025] The microneedle and the working electrode are both arranged on the electrode substrate;

[0026] microneedles, used to obtain juice from target plants;

[0027] a working electrode for generating biological information of an electrical signal based on the juice and sending the biological information to a signal converter;

[0028] The signal converter converts the biological information of the electrical signal into the biological information of the digital signal, and sends the biological information of the digital signal to the central control module.

[0029] In some preferred embodiments of the present invention, the diameter of the microneedle is 100-200 μm, and the length is 0.01 mm-0.05 mm.

[0030] In some preferred embodiments of the present invention, a reaction enzyme is immobilized on the working electrode;

[0031] The reaction enzyme includes at least one of the following: glucose oxidase, isocitrate dehydrogenase or ascorbate oxidase.

[0032] In some preferred embodiments of the present invention, the device further comprises: a temperature and humidity sensing module;

[0033] The temperature and humidity sensing module includes: a fixed sheet, a camera, a variable length line, a humidity sensitive material and a temperature sensitive material;

[0034] The fixed sheet is first connected to the moisture sensitive material through a variable length; the moisture sensitive material is connected to the temperature sensitive material;

[0035] A fixing sheet, used for fixing and connecting with the leaves of the target plant;

[0036] A camera is used to capture images of moisture-sensitive materials and temperature-sensitive materials.

[0037] In a second aspect, the present invention provides a wearable multi-element collaborative plant growth monitoring system, comprising: an external communication device and the wearable multi-element collaborative plant growth monitoring device provided in the first aspect.

[0038] The present invention brings the following beneficial effects:

[0039] The present invention provides a wearable multi-element collaborative plant growth monitoring device and system, comprising: a gas sensor module, a laser sensor module, an electrochemical sensor module and a central control module; the gas sensor module is used to detect gas information of the surrounding environment of the target plant and send the gas information to the central control module; wherein the gas information includes at least one of the following: ethylene gas concentration, carbon dioxide gas concentration or nitric oxide gas concentration; the laser sensor module is used to obtain the diameter of the stem of the target plant and send the diameter of the stem of the target plant to the central control module; the central control module is used to generate an action signal if the gas information meets the preset conditions and / or the diameter of the stem of the target plant meets the preset growth conditions, and to move the target plant to the central control module. The action signal is sent to the electrochemical sensor module; the electrochemical sensor module is used to receive the action signal, collect the juice of the target plant, obtain the biological information of the target plant based on the juice, and send the biological information to the central control module; wherein the biological information includes at least one of the following: sugar content, acidity or vitamin C content; the diameter of the stem of the target plant is obtained by the laser sensor module, and the biological information is collected by the electrochemical sensor module after the gas information and / or the stem diameter reaches the preset judgment condition, so that multiple modules can work together and focus on key detection nodes, thereby improving the accuracy of plant growth monitoring and the effectiveness of detection results, improving the accuracy and efficiency of planting in agricultural work, and achieving improvements such as increased production, improved quality and energy saving. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0041] Figure 1 A schematic diagram of the structure of a wearable multi-element collaborative plant growth monitoring device provided by an embodiment of the present invention;

[0042] Figure 2 A top view of the structure of a gas sensor module provided by an embodiment of the present invention;

[0043] Figure 3 A left side view of the structure of a gas sensor module provided by an embodiment of the present invention;

[0044] Figure 4 A gas sensor signal schematic diagram provided by an embodiment of the present invention;

[0045] Figure 5 A schematic diagram of the structure of a laser sensor module provided by an embodiment of the present invention;

[0046] Figure 6 A schematic diagram of the structure of an electrochemical sensor module provided by an embodiment of the present invention;

[0047] Figure 7 A schematic diagram of the structure of a microneedle provided in an embodiment of the present invention;

[0048] Figure 8 A schematic diagram of biological information of a target plant provided by an embodiment of the present invention;

[0049] Fig. 9 A schematic diagram of the structure of a temperature and humidity sensor module provided by an embodiment of the present invention;

[0050] Fig.10 A schematic diagram of the working logic of a wearable multi-element collaborative plant growth detection device provided in an embodiment of the present invention.

[0051] Icons: 1000-temperature and humidity sensor module; 1100-fixed sheet; 1200-polypropylene wire; 1300-humidity sensitive material; 1400-temperature sensitive material; 1500-camera; 2000-gas sensor module; 2110-ethylene gas sensor; 2111-Pd / Ti3C2T x Composite material; 2120-carbon dioxide gas sensor; 2130-nitric oxide gas sensor; 2200-flexible gas substrate; 2300-gas signal transmission processing unit; 3000-electrochemical sensor module; 3100-electrode substrate; 3200-microneedle; 3300-reference electrode; 3400-working electrode; 3500-counter electrode; 3700-signal converter; 4000-laser sensor module; 4100-laser transmitter; 4200-laser receiver; 5000-central control module. DETAILED DESCRIPTION

[0052] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings here can be arranged and designed in various different configurations.

[0053] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0054] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0055] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc. indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, or the positions or positional relationships in which the product of the invention is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific position, be constructed and operated in a specific position, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

[0056] In addition, the terms "horizontal", "vertical", "overhanging" and the like do not mean that the components are required to be absolutely horizontal or overhanging, but can be slightly tilted. For example, "horizontal" only means that its direction is more horizontal than "vertical", and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0057] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0058] The plant growth process is also closely related to some gases. For example, ethylene can affect fruit ripening and adversity response, carbon dioxide can affect photosynthesis, and nitric oxide, as an endogenous signal molecule, can also regulate plant growth, adversity response and other processes. Traditional gas detection mainly uses portable spectrometers, electronic noses, etc., but they can often only detect data on the overall environment and cannot detect the amount of microenvironmental gas in which plants grow. In other words, the detected data is easily diluted by the environment, and accurate sampling around the plant is not achieved. The method of using gas chromatography equipment to analyze gases is costly, complex to operate, and takes a long time to analyze.

[0059] Detecting changes in plant stem diameter has many implications for plant growth. It can not only reflect the moisture status of crops, provide a scientific basis for irrigation, optimize irrigation management, and improve water resource utilization efficiency, but also conduct in-depth research on plant growth mechanisms, monitor the growth process, analyze the impact of environmental factors, and reveal the relationship between growth and the environment. At the same time, by monitoring changes in stem diameter, problems in plant growth can be discovered in a timely manner, growth regulation can be carried out, healthy growth can be promoted, crop yield and quality can be improved, and a basis for variety screening can be provided. The traditional method of detecting plant stem diameter is to measure by vernier calipers and other methods, which is prone to insufficient accuracy due to poor measurement position, and cannot achieve long-term real-time monitoring.

[0060] During the growth of fruits, testing their sugar content, pH value, vitamin content and other information can provide feedback on fruit quality, guide the adjustment of parameters such as fertilizer, water, and light, and achieve quality improvement and intelligent planting. Traditional technology mainly conducts destructive testing after collecting fruits, and cannot provide real-time feedback on planting plans. Portable non-destructive testing equipment based on spectroscopy cannot accurately detect the above indicators due to obstructions of the peel and wax layer. High-performance liquid chromatography is a method for detecting sugar content, acidity and vitamin C in plant fruits, but its equipment cost is high, the operation is complicated, and the analysis time is long.

[0061] The stomata of plants are mainly located under the leaves. Due to the influence of transpiration, the opening and closing of stomata will affect the humidity changes between plant leaves. Temperature is the main factor in the opening and closing response of plant stomata. Measuring the temperature and humidity of the microenvironment between plant leaves can infer the opening and closing state of stomata, and then deduce the transpiration of plants, which helps to understand the growth status and water absorption of plants. However, this microenvironmental condition is easily affected by the planting environment, and wearable detection is needed to obtain instructive data. Traditional methods for measuring plant leaf temperature, such as the thermocouple method, require physical contact and may cause slight damage to the leaves; installation and maintenance are relatively complicated; such as the infrared temperature measurement method, although no contact is required, it is greatly affected by environmental factors (such as reflection and absorption), and needs to be calibrated at any time to improve accuracy. The traditional method of measuring plant leaf humidity is detected by a capacitive humidity sensor, which has physical contact with the plant, causing slight damage to the leaves. During the contact process, the stomata are easily blocked, resulting in a decrease in detection accuracy, and the installation and maintenance are relatively complicated; such as the infrared humidity sensor detection method, although no contact is required with the plant, it is greatly affected by environmental factors (such as reflection and absorption), and needs to be calibrated at any time to improve accuracy.

[0062] The present invention provides a wearable multi-element collaborative plant growth monitoring device and system, which obtains gas information of the surrounding environment of the target plant through a gas sensor module 2000, obtains the stem diameter of the target plant through a laser sensor module 4000, and collects the gas information and / or the stem diameter after reaching a preset judgment condition through an electrochemical sensor module 3000, so that multiple modules can work together and focus on key detection nodes, thereby improving the accuracy of plant growth monitoring and the effectiveness of detection results, and enhancing user experience. It can not only provide strong technical support for agricultural production, but also help promote the development of modern agriculture in a more intelligent and sustainable direction.

[0063] Some embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other.

[0064] Embodiment 1

[0065] The embodiment of the present invention provides a wearable multi-element collaborative plant growth monitoring device, see Figure 1 The structure diagram of a wearable multi-element collaborative plant growth monitoring device provided by an embodiment of the present invention is shown, and the device includes: a gas sensor module 2000, a laser sensor module 4000, an electrochemical sensor module 3000 and a central control module 5000; the gas sensor module 2000 is used to detect the gas information of the surrounding environment of the target plant and send the gas information to the central control module 5000; wherein the gas information includes at least one of the following: ethylene gas concentration, carbon dioxide gas concentration or nitric oxide gas concentration.

[0066] In some preferred embodiments of the present invention, the plant sample is properly processed, and the microelectrode is inserted into the plant sample to ensure that the electrode is in full contact with the sample. The connected electronic device measures the electrical signal on the electrode. According to the measured electrical signal, combined with the calibration curve, the sugar content in the plant sample is calculated. The pH is measured using a pH microelectrode. Specifically, the microelectrode can be directly inserted into the tissue or cell to be tested to measure the pH value. When measuring the intracellular pH value, the microelectrode can be inserted into the cell to be tested, and then the potential difference value between the two electrodes is read. After the corresponding calculation, the measured pH value can be obtained. The 2,6-dichloroindophenol method is used to quickly determine vitamin C. Specifically, a certain amount of fruit juice is taken and diluted with an appropriate amount of water. Titrate with 2,6-dichloroindophenol solution until the blue color no longer disappears. Record the volume of the consumed 2,6-dichloroindophenol solution, and calculate the vitamin C content. The laser sensor module 4000 uses laser time of flight (ToF) technology to determine the diameter of the stem. The laser transmitter 4100 emits a laser pulse, which is reflected on the surface of the stem. The receiver receives the reflected laser pulse. The time it takes for a laser pulse to be emitted and returned is measured, and the distance is calculated using the speed of light.

[0067] Furthermore, in some preferred embodiments of the present invention, the gas sensing module 2000 includes: a flexible gas substrate 2200, a gas signal transmission processing unit 2300 and at least one gas sensor; the gas signal transmission processing unit 2300 and the gas sensor are arranged on the flexible gas substrate 2200; the gas sensor is used to generate a gas electrical signal based on the concentration of the target gas, and send the gas electrical signal to the gas signal transmission processing unit 2300; the gas signal transmission processing unit 2300 is used to amplify the gas electrical signal and transmit it to the central control module 5000.

[0068] Furthermore, in some preferred embodiments of the present invention, the gas sensor includes at least one of the following: a flexible ethylene gas sensor 2110 , a flexible carbon dioxide gas sensor 2120 , or a flexible nitric oxide gas sensor 2130 .

[0069] For details, see Figure 2 The structure top view of a gas sensor module provided by an embodiment of the present invention is shown in FIG. Figure 3 The structure of a gas sensor module provided by an embodiment of the present invention is shown in the left view, where the gas sensor module 2000 includes a flexible ethylene gas sensor 2110, a flexible carbon dioxide gas sensor 2120, a flexible nitric oxide gas sensor 2130, a signal transmission processing device and a flexible gas substrate 2200. The gas sensor module 2000 is tied to the upper part of the plant rhizome through a flexible polydimethylsiloxane material to monitor the content of different gas components in the plant growth environment and provide feedback on the growth status of the plant.

[0070] Specifically, the flexible ethylene gas sensor 2110 is composed of Pd / Ti3C2T x Composite material 2111. This material will change resistance when ethylene concentration changes, thus causing output voltage changes. Pd / Ti3C2T x The addition of Pd nanoparticles formed a Pd / Ti heterostructure, and Pd modified Ti3C2T x , Pd nanoparticles can be distributed in Ti3C2T x Surface, when Pd / Ti3C2T x The best response to ethylene is shown when the volume ratio is 1:10-1:15. This not only enhances the conductivity of the material, but also improves the electron mobility by lowering the Schottky barrier. x When the material is formed, the π bonds between the ethylene molecules and the Pd nanoparticles interact with the electron density of the metal orbitals, causing a change in resistance.

[0071] The flexible carbon dioxide gas sensor 2120 is made of a mixture of zirconium oxide and graphene. This composite material can improve the conductivity and mechanical properties of zirconium oxide. When preparing the material, a porous nano zirconium oxide film with a pore size of 10nm-100nm is first sputtered on the substrate material by vapor deposition, and then the graphene material is modified on the surface of the material by chemical deposition, so that the graphene thickness is 2-10nm, so as to maximize the conductivity and the adsorption effect of carbon dioxide. This material will change its resistance when the concentration of carbon dioxide changes, thereby causing a change in the output voltage. When zirconium oxide materials adsorb carbon dioxide, they will form compounds such as carbonates or bicarbonates, which will affect the conductivity of zirconium oxide. When the concentration of carbon dioxide changes, the formation and decomposition rate of these compounds will also change, resulting in changes in conductivity. By modifying the surface of zirconium oxide with graphene, on the one hand, the conductivity of the material can be improved, and on the other hand, it can be used as a catalyst to accelerate chemical reactions and improve the response speed of the sensor.

[0072] The flexible nitric oxide gas sensor 2130 is made of a mixture of tin oxide and graphene. A porous nano-tin oxide film with a pore size of 10nm-100nm is first sputtered on the substrate material by vapor deposition, and then the graphene material is modified on the surface of the material by chemical deposition to make the graphene thickness 2-10nm, so as to maximize the conductivity and the adsorption effect of nitric oxide. This composite material can improve the conductivity and mechanical properties of tin oxide. The resistance of this material changes when the concentration of nitric oxide changes, thereby causing a change in the output voltage. The tin oxide material adsorbs nitric oxide molecules and reacts with oxygen ions on the surface to form nitrate ions. This process consumes oxygen ions on the surface, reduces electron capture on the surface, and thus increases the conductivity of the material. When the concentration of nitric oxide decreases, the nitric oxide molecules adsorbed on the surface will gradually desorb and return to the initial state, and the conductivity will also be restored. By modifying the surface of tin oxide with graphene, the conductivity of the material can be improved on the one hand, and on the other hand, it can be used as a catalyst to accelerate chemical reactions and improve the response speed of the sensor.

[0073] The signal generated by the flexible gas sensor is the change value of the resistance signal. The data terminal reflects the change in the gas concentration in the environment by amplifying the voltage change value caused by the resistance signal value. The flexible gas substrate 2200 is made of polypropylene material, which has excellent waterproof and ductile properties and is used to make the detection module fit the plant stem. The gas signal transmission processing unit 2300 is used to process the data generated by the flexible gas sensor and output the result.

[0074] For example, see Figure 1 The flexible gas sensor module 2000 is fixed above the plant stem to test the gas concentration environment around the plant.

[0075] Changing the ethylene gas concentration in the surrounding environment, Pd / Ti3C2T in the ethylene gas sensor 2110 x The composite material 2111 enriches the ethylene gas in the environment, converts the gas concentration into resistance, and outputs it as voltage intensity. The gas signal transmission processing unit 2300 transmits the data to the data processing center for data processing, and then uploads the data to the mobile terminal, so that we can view the data at any time on the mobile terminal.

[0076] By changing the carbon dioxide gas concentration in the surrounding environment, the zirconium oxide in the carbon dioxide gas sensor 2120 enriches the carbon dioxide gas in the environment, converts the gas concentration into resistance, and outputs it as voltage intensity. The gas signal transmission processing unit 2300 transmits the data to the data processing center for data processing, and then uploads the data to the mobile terminal, allowing us to view the data at any time on the mobile terminal.

[0077] By changing the concentration of nitric oxide gas in the surrounding environment, the tin oxide in the nitric oxide gas sensor 2130 enriches the nitrogen dioxide gas in the environment, and converts the gas concentration into resistance size and outputs it as voltage intensity. The gas signal transmission processing unit 2300 transmits the data to the data processing center for data processing, and then uploads the data to the mobile terminal. This allows us to view the data at any time on the mobile terminal.

[0078] For further information, see Figure 4 The schematic diagram of a gas sensor signal provided by an embodiment of the present invention is shown in FIG. 1 , from top to bottom, respectively, Pd / Ti3C2T x Time-dependent curves of composite materials, zirconium oxide, and tin oxide.

[0079] The laser sensor module 4000 is used to obtain the diameter of the stem of the target plant and send the diameter of the stem of the target plant to the central control module 5000.

[0080] Furthermore, in some preferred embodiments of the present invention, the laser sensor module 4000 includes: a laser transmitter 4100 and a laser receiver 4200 that are relatively arranged, and a laser data processing unit; the laser transmitter 4100 is used to emit a laser beam; a portion of the laser beam is blocked by the stem of the target plant; the laser receiver 4200 is used to receive the laser beam; and the laser data processing unit is used to determine the diameter of the stem of the target plant based on the laser beam received by the laser receiver 4200.

[0081] For details, see Figure 5The structure diagram of a laser sensor module provided by an embodiment of the present invention is shown, and the laser sensor module 4000 includes a laser transmitter 4100, a laser receiver 4200 and a data processing unit. The laser transmitter 4100 includes an infrared laser diode, an optical lens, and a power supply. The optical lens is used to focus the laser beam to ensure that the diameter and intensity of the laser beam meet the requirements, and the power supply provides a stable operating voltage for the laser diode. The infrared laser diode is responsible for emitting the laser beam, and the laser receiver 4200 includes a photodetector, an amplifier, and an analog-to-digital converter. The photodetector is a photodiode. When the laser beam passes through the plant stem, it will be partially blocked, forming a light spot at the receiving end. The photodiode can receive the formed light spot signal, the amplifier is used to amplify the weak signal received by the photodetector, and the analog-to-digital converter can convert the optical signal into a digital signal, which is convenient for subsequent data transmission and analysis.

[0082] The calculation function of plant stem diameter is as follows:

[0083] ;

[0084] Where α is the absorption coefficient of the plant material, the initial intensity of the laser beam is I0, the intensity after passing through the plant stem is I, the diameter of the plant stem is d, and the unit of I is W / cm 2 , d is in mm. By solving the above equation, d can be obtained.

[0085] Furthermore, in some preferred embodiments of the present invention, the laser sensor module 4000 also includes: a first position adjustment mechanism and a second position adjustment mechanism; the laser emitter 4100 is arranged on the first position adjustment mechanism; the laser receiver 4200 is arranged on the second position adjustment mechanism; the first position adjustment mechanism is used to adjust the position of the laser emitter 4100 relative to the target plant; the second position adjustment mechanism is used to adjust the position of the laser receiver 4200 relative to the target plant.

[0086] Specifically, the position adjustment mechanism includes: a linear track, an adjustment structure, and a shell material. The linear track is used to stabilize the position of the laser displacement sensor to ensure that its relative position with the stem remains unchanged. The adjustment structure is a screw driven by an electric motor, which allows the position of the laser transmitter 4100 and the receiver to be adjusted to adapt to plant stems of different diameters. The shell material is made of plastic and has a certain protection level to protect the internal electronic components from environmental factors.

[0087] For example, see Figure 1, the laser transmitter 4100 emits a narrow laser beam, which is focused by an optical lens and irradiated to one side of the plant stem. The laser beam penetrates the plant stem and reaches the other side. The diameter of the plant stem will affect the attenuation of the laser beam. The laser receiver 4200 is located on the other side of the plant stem and receives the laser signal that passes through the plant stem. The photodetector converts the received optical signal into an electrical signal. The amplifier amplifies the weak electrical signal received by the photodetector and then converts it into a digital signal through the signal converter 3700. The data processing center processes the received digital signal and calculates the attenuation of the laser beam. By analyzing the attenuation of the laser signal, combined with the known laser power and the absorption coefficient of the plant material and other factors, the actual diameter of the plant stem is calculated. The final measurement result is transmitted to the mobile terminal via wireless transmission, and the data can be viewed at any time on the mobile terminal.

[0088] The central control module 5000 is used to generate an action signal and send the action signal to the electrochemical sensor module 3000 if the gas information meets the preset conditions and / or the diameter of the stem of the target plant meets the preset growth conditions.

[0089] The specific central control module 5000 includes a data processing center, a mobile terminal, and a microprocessor. The data processing center can store, process, and output the received data. The mobile terminal can view the data at any time. The data processing center and the mobile terminal transmit wirelessly via Bluetooth signals. As the "brain" of the entire system, the central control module 5000 receives all input information from sensors and analyzes and judges according to preset rules. When any situation beyond the normal range is found - whether it is abnormal gas composition or plant growth is hindered - the alarm mechanism will be triggered and the corresponding action instructions will be generated for the next link.

[0090] For example, when the values ​​of the daily change in stem diameter for nearly five days are plotted and the slope of the fitting curve is greater than 3, it is considered that the plant has entered a rapid growth stage. At this time, the electrochemical sensor module 3000 is used to avoid the impact of premature intervention. When the concentration of ethylene and the humidity above the leaves increase for 3 consecutive hours, and the carbon dioxide concentration keeps decreasing for 3 consecutive hours, an electrochemical module test is performed to minimize damage to the fruit and provide true feedback on whether the metabolic state of the fruit is good and whether the quality meets expectations.

[0091] The electrochemical sensor module 3000 is used to receive action signals, collect juice from the target plant, obtain biological information of the target plant based on the juice, and send the biological information to the central control module 5000; wherein the biological information includes at least one of the following: sugar content, acidity or vitamin C content.

[0092] Further, in some preferred embodiments of the present invention, see Figure 6The structure schematic diagram of an electrochemical sensor module provided by an embodiment of the present invention is shown, and the electrochemical sensor module 3000 includes: an electrode substrate 3100, a microneedle 3200, a working electrode 3400 and a signal converter 3700; the microneedle 3200 and the working electrode 3400 are both arranged on the electrode substrate 3100; the microneedle 3200 is used to obtain the juice of the target plant; the working electrode 3400 is used to generate biological information of the electrical signal based on the juice, and send the biological information to the signal converter 3700; the signal converter 3700 converts the biological information of the electrical signal into biological information of a digital signal, and sends the biological information of the digital signal to the central control module 5000.

[0093] Specifically, in some preferred embodiments of the present invention, the microneedle 3200 is disposed on the electrode substrate 3100 and does not overlap with the working electrode 3400 , and the height of the microneedle 3200 only needs to be higher than the working electrode.

[0094] Furthermore, in some preferred embodiments of the present invention, a reaction enzyme is fixed on the working electrode 3400; the reaction enzyme includes at least one of the following: glucose oxidase, isocitrate dehydrogenase or ascorbic acid oxidase.

[0095] Specifically, PI film is selected as a flexible substrate material to ensure that the sensor can be attached to the surface of the fruit and used to carry different electrodes. Gold is selected as the working electrode 3400 and the counter electrode 3500 materials, and the electrode shape is formed on the PI film by an electrodeposition method. Silver / silver chloride material is selected as the reference electrode 3300 material, and is also prepared on the substrate by an electrodeposition method. The three electrodes together constitute a three-electrode system for realizing electrochemical detection.

[0096] The working electrode 3400 can sense the target substance and generate an electrical signal proportional to the concentration of the target substance, and provide a reaction interface so that the target substance reacts with the active substance on the electrode surface.

[0097] In some preferred embodiments of the present invention, the electrochemical sensor module 3000 further includes: a reference electrode 3300 and a counter electrode 3500, both of which are arranged on the electrode substrate 3100, and the reference electrode 3300 is used as a potential reference point to ensure that the measured potential change is caused by the target substance, rather than by other factors. Provide a stable benchmark to make electrochemical measurements more accurate and reliable. The counter electrode 3500 is used to balance the charge on the working electrode 3400 to ensure the continuous flow of current. Together with the working electrode 3400 and the reference electrode 3300, a complete three-electrode system is formed to ensure the normal progress of the electrochemical reaction.

[0098] Microneedles are a series of tiny spike-like structures. In some preferred embodiments of the present invention, the microneedles are located on the surfaces of the working electrode, counter electrode and reference electrode of the electrochemical sensor. The material is the same as that of the electrode in which they are located. They are prepared on the corresponding electrodes by vacuum sputtering and other methods, so as to slightly damage the peel without hindering the growth of the plant and make the three electrodes (working electrode, counter electrode, reference electrode) fully contact with the juice of the target plant, providing a prerequisite for electrochemical sensing.

[0099] Further, the working electrode 3400 is a gold electrode modified with different enzymes, and the modified enzymes include sugar-sensitive materials, pH-sensitive materials, and vitamin C-sensitive materials. The modification of the working electrode 3400 is performed after the microneedle 3200 is prepared to avoid damage to the enzyme electrode when preparing the microneedle 3200. Different enzymes are fixed on the working electrode 3400, which will produce redox reactions with different detection targets. When the voltage is correct, electron transfer will occur, resulting in current changes. Therefore, three different substances can be quantified by one DPV scan and the corresponding current intensity of three different voltages. Since the binding efficiency of different enzymes and substances is different, and the content of corresponding substances in fruits is different, and the mutual interference between the three enzymes must be considered, the three enzymes need to be mixed in a certain proportion when they are mixed and fixed on the surface of the working electrode 3400. The optimal range is that the ratio of glucose oxidase: isocitrate dehydrogenase: ascorbic acid oxidase is 2:3:5. In addition, in order to improve the detection effect, the above-mentioned electrodes all add a His tag to the N-terminus of the enzyme before enzyme fixation. Compared with other enzyme electrodes, this method can achieve a unified enzyme fixation direction, that is, the position of the His tag is used to guide the enzyme to be fixed on the electrode surface in a fixed direction. The significance of this is to make the side of the enzyme that is conducive to binding with the target face up, making binding easier, thereby improving detection efficiency. Compared with the general method (using the hydroxyl or carboxyl groups on the enzyme surface for fixation), it is more directional.

[0100] The sugar-sensitive material is glucose oxidase, which is an enzyme that is highly specific to glucose and can be fixed to the electrode surface in a directional manner. First, a His tag is introduced through genetic engineering, and a tag is introduced at the N-terminus of the glucose oxidase gene sequence. Then an expression vector is constructed, the gene is cloned, and the constructed expression vector is transferred into Escherichia coli. Under the conditions of 37°C and a shaker speed of 200 rpm, the expression of the enzyme is induced using the inducer 0.5MIPTG. The bacteria are collected, resuspended with lysis buffer (50mM Tris-HCl, pH 8.0, 300mM NaCl, 10mM imidazole), and the cells are ultrasonically broken. Centrifuge at 12,000 rpm for 15 minutes and collect the supernatant. The supernatant is passed through a nickel ion chelating column resin column, and the purified enzyme is eluted with an elution buffer (50mM Tris-HCl, pH8.0, 300mM NaCl, 250mM imidazole). The electrode is cleaned with ultrapure water and ethanol to remove impurities and contaminants on the surface. Use an ultrasonic cleaner to further clean the electrode surface to ensure that the surface is clean and free of contamination. Soak the electrode in the nickel ion chelate column resin solution and incubate at room temperature for 1-2 hours. Wash the electrode with phosphate buffer (pH7.0-7.4) to remove excess nickel ion chelate column resin. Dilute the purified enzyme solution to 1 mg / mL and prepare it with phosphate buffer. Add the enzyme solution dropwise to the modified electrode surface, ensuring that the solution evenly covers the electrode and incubate at room temperature for 1-2 hours. Wash the electrode with phosphate buffer to remove unbound enzyme. Block the electrode surface with 1% bovine serum albumin solution and incubate at room temperature for 1 hour to reduce nonspecific adsorption. Wash the electrode again with phosphate buffer to remove excess bovine serum albumin. The enzyme was successfully immobilized on the electrode surface.

[0101] The pH sensitive material is isocitrate dehydrogenase, which can catalyze the oxidative decarboxylation of isocitrate to generate α-ketoglutarate and carbon dioxide, and convert NAD + or NADP + Reduced to NADH or NADPH. Can be fixed to the enzyme surface in the same way.

[0102] The vitamin C sensitive material is ascorbic acid oxidase, which is an enzyme highly specific to vitamin C and can be directionally fixed to the electrode surface using the same method as above.

[0103] The key parameters that affect the final fixation of the enzyme concentration are: the concentration of the initial enzyme solution, the fixation time, the selection of the cross-linking agent, and the use of a protective agent such as bovine serum albumin. To improve the detection efficiency, the embodiments of the present invention improve the detection efficiency by directional fixation of the enzyme, determining suitable immobilization conditions, and optimizing electrochemical parameters.

[0104] In some preferred embodiments of the present invention, a protective film is covered on the surface of the working electrode 3400. The protective layer is polyvinyl alcohol. Polyvinyl alcohol has good biocompatibility, chemical stability and film-forming properties, and can effectively prevent enzyme inactivation.

[0105] The signal converter 3700 includes a voltage source, a transimpedance amplifier, a microcontroller, and an analog-to-digital converter. The voltage source is capable of accurately outputting a precise differential pulse voltage. The transimpedance amplifier can amplify a weak current signal. The microcontroller is used to generate and control the differential pulse voltage. The analog-to-digital converter can convert the current signal into a digital signal, which is convenient for subsequent data transmission and analysis.

[0106] In some preferred embodiments of the present invention, an electrochemical workstation is used to perform a DPV test, and the DPV parameter settings are: starting potential: -0.2V; ending potential: 1.0V; step potential: 5mV; pulse amplitude: 50mV; pulse width: 50ms; pulse interval: 500ms. The electrochemical workstation is started and the DPV test program is run. The detection results show that the corresponding voltage of vitamin C is 0.2V, the corresponding voltage of isocitric acid is 0.3V, and the corresponding voltage of glucose is 0.4V.

[0107] The relationship between current and concentration in the electrochemical sensor is I=k×[C]+0.1, where I is the current in μA and C is the concentration in mM. k is the proportional constant, which varies for different substances. The proportional constant for glucose is 0.2, the proportional constant for isocitric acid is 0.15, and the proportional constant for vitamin C is 0.1.

[0108] Further, in some preferred embodiments of the present invention, see Figure 7 The schematic diagram of the structure of a microneedle provided by an embodiment of the present invention is shown, and the diameter of the microneedle 3200 is 100-200 μm and the length is 0.01 mm-0.05 mm.

[0109] Specifically, microneedle 3200 is made of thermoplastic polyurethane elastomer material and is printed using a photocurable thick modeling printer. The diameter of microneedle 3200 is 100-200μm and the length is 0.01mm-0.05mm to ensure that it can penetrate the fruit skin without causing excessive damage, and squeeze out a small amount of juice to provide the electrolyte environment required for detection. The shape of microneedle 3200 is conical to optimize its puncture performance and mechanical strength. A layer of gold film is deposited on the tip of microneedle 3200 using an electroplating method to form multiple independent electrodes.

[0110] For example, see Figure 1, attach the electrochemical sensor module 3000 to the surface of the plant, and collect liquid samples from the plant through the microneedle 3200. The sugar-sensitive material on the working electrode 3400 reacts with the sugar in the plant to generate hydrogen peroxide. The pH-sensitive material reacts with the isocitric acid in the plant to generate NADH and NADPH. The vitamin C-sensitive material reacts with the vitamin C in the plant to generate dehydroascorbic acid. The signal converter 3700 generates a series of short-term differential pulse voltages and applies them to the working electrode 3400. The generated hydrogen peroxide, NADH and dehydroascorbic acid are oxidized on the electrode surface to generate a current signal. The signal converter 3700 converts the current signal into a digital signal, and the data processing center processes the data and transmits the data to the mobile terminal via wireless transmission. See Figure 8 The schematic diagram of biological information of a target plant provided by an embodiment of the present invention is shown, the horizontal axis is the potential, and the vertical axis is the current. The mobile terminal displays the sugar content, acidity, and vitamin C content in the plant, thereby reflecting the sugar content, acidity, and vitamin C content of the plant.

[0111] The wearable multi-factor collaborative plant growth monitoring device provided in the embodiment of the present invention uses multiple detection methods in a collaborative way to infer the growth status of plants through the humidity of leaves, the diameter of stems and the content of key gas hormones. On the one hand, it can correctly reflect the growth status of plants through cross-linking analysis of multiple factors (more accurate than a single factor, because a single indicator of a plant is often affected by multiple factors); on the other hand, it can guide the sampling time of the electrochemical detection module, reduce the detection frequency, and reduce the impact on the fruit.

[0112] Furthermore, in some preferred embodiments of the present invention, the device also includes: a temperature and humidity sensing module 1000; the temperature and humidity sensing module 1000 includes: a fixed sheet 1100, a camera 1500, a variable length line, a humidity sensitive material 1300 and a temperature sensitive material 1400; the fixed sheet 1100 is first connected to the humidity sensitive material 1300 through a variable length; the humidity sensitive material 1300 is connected to the temperature sensitive material 1400; the fixed sheet 1100 is used to be fixedly connected to the leaves of the target plant; the camera 1500 is used to collect images of the humidity sensitive material 1300 and the temperature sensitive material 1400.

[0113] For details, see Fig. 9 The structural diagram of a temperature and humidity sensor module provided by an embodiment of the present invention is shown, and the fixing sheet 1100 is used to fix the temperature and humidity sensor module 1000 below the surface of the leaf, so that the temperature and humidity sensor module 1000 can be suspended below the leaf. The fixing sheet 1100 is composed of a sticky film material such as polyvinyl chloride tape. This flexible material can ensure that the leaf will not be damaged when the sensor is fixed, and will not affect the normal growth of the plant during the use of the sensor.

[0114] The variable length wire is used to change the height of the entire device, so that the entire device can be closer to the optimal measurement position and minimize the impact on plant growth. The variable length wire is composed of polypropylene wire 1200, which has good corrosion resistance and can be replaced at any time, which can increase the service life of the temperature and humidity sensor module 1000.

[0115] The humidity sensitive material 1300 can sensitively detect humidity and can bend to varying degrees when humidity changes. The humidity sensitive material 1300 is a mixture of graphene oxide wire and polyvinyl alcohol, with a mixing ratio of graphene oxide wire: polyvinyl alcohol 1:7~1:8 (mass ratio). The composite material prepared in this way can make the material have more excellent mechanical properties and humidity sensitivity. Under different humidity environments, the graphene oxide wire will absorb water molecules in the air, and these water molecules will enter the interlayer of graphene oxide, resulting in an increase in the interlayer spacing, causing the material to bend to varying degrees, and finally present a contracted or stretched shape. The original length of the humidity sensitive material 1300 is 3cm, and the relationship between humidity and length changes linearly, with a linear relationship of y=3-1.2x, where y is the length of the humidity sensitive material 1300 and x is the percentage humidity. The temperature sensitive material 1400 can sensitively detect temperature and will produce color changes from light red to dark red when the temperature changes.

[0116] The temperature-sensitive material 1400 is a mixture of polythiophene and graphene in a ratio of polythiophene: graphene 2:5~3:5 (mass ratio). After mixing, the material is shaped into a rhombus and connected to the humidity-sensitive material 1300 through polyacrylic acid, and the two are connected by low-temperature sintering, drying and fixing. The composite material prepared in this way can enhance the conductivity and thermal stability of the material. The color of polythiophene is closely related to its absorption spectrum. When polythiophene is at different temperatures, the electron energy levels in its molecules will change. This change in energy level affects the material's ability to absorb light with a wavelength of 600-750nm, resulting in the observed color difference. As the temperature rises, the color changes from light red to dark red.

[0117] The camera 1500 is used to observe the changes of the humidity sensitive material 1300 and the temperature sensitive material 1400. The camera 1500 identifies the humidity sensitive material 1300. When the humidity changes, the bending degree of the humidity sensitive material 1300 will change. The camera 1500 takes a picture and transmits it to the data terminal. The data terminal analyzes the bending degree and matches the corresponding data humidity. The camera 1500 identifies the temperature sensitive material 1400. When the temperature changes, the color of the temperature sensitive material 1400 will change. The camera 1500 takes a picture of the color change and transmits it to the data terminal. The data terminal analyzes the color degree and matches the corresponding data temperature. The chromaticity is judged by detecting the refraction intensity of the 650-700nm wavelength light (the wavelength range is slightly narrower than the color change range to improve the detection sensitivity and accuracy). The relationship between temperature and light intensity in the temperature sensitive material 1400 is y=620+1.3(x-270), where y is the light intensity (candela per square meter) and x is the temperature (Kelvin).

[0118] For example, see Figure 1 , select a suitable blade to stick the fixed sheet 1100 under the blade, and adjust the polypropylene line 1200 to put the whole device in a suitable position. Change the ambient humidity from 10% to 50% and then to 90%, and observe that the humidity sensitive material 1300 produces different degrees of bending. As the humidity increases, the camera 1500 observes that the bending degree of the graphene oxide line becomes larger and larger. And transmit the data to the central control module 5000 for processing and output to the mobile terminal. So that we can view the data at any time on the mobile terminal. Change the ambient temperature, gradually adjust the temperature from 15 degrees Celsius to 45 degrees Celsius, and observe that the color of the temperature sensitive material 1400 changes. When the temperature gradually rises, the camera 1500 observes that the color of polythiophene changes from light red to dark red. And transmit the data to the central control module 5000 for processing and output to the mobile terminal. So that we can view the data at any time on the mobile terminal.

[0119] The wearable multi-element collaborative plant growth monitoring device provided in the embodiment of the present invention reflects the microenvironment of plant growth based on the monitoring data of the temperature and humidity sensor module, so as to infer the opening and closing status of the stomata under the plant leaves, which is helpful to combine with the other detection modules mentioned above in this embodiment to carry out some scientific research work to infer the research on plant growth or adversity response mechanism.

[0120] In the specific implementation process, see Fig.10The working logic diagram of a wearable multi-element collaborative plant growth detection device provided by an embodiment of the present invention is shown. When the plant stomata are opened, the humidity of the microenvironment around the leaves (which can be measured by the temperature and humidity sensing module) rises due to transpiration, and the humidity of the microenvironment around the plant can be measured by the temperature and humidity sensing module; and it is known that nitric oxide promotes stomatal opening when the concentration (obtained by the gas sensing module) is low, and promotes stomatal closing when it is high; it is also known that the plant stomata show a situation in which the derivative of the stem diameter change (determined by the continuous measurement results of the laser sensing module) is negative for three consecutive days (i.e., negative diameter growth) in the period before the stomata are closed in response to drought. The observation and recording of the remaining sensor modules can be started after the above situation occurs in the data recorded by any sensor module. The start can also be after the above situation occurs in the data recorded by any sensor module, the data previously recorded by the remaining sensor modules are obtained, and the data of multiple modules are combined to judge the open and closed state of the plant stomata, which is more accurate.

[0121] On this basis, the length of time the stomata are open and closed and the daily change in stem diameter can be used to determine whether the plant is in a good growth state, thereby optimizing the planting method. This method makes judgments by directly quantifying the growth state of the plant, which can more accurately guide method optimization than traditional technologies that rely on human experience or soil environment testing.

[0122] Furthermore, combined with the readings of ethylene and carbon dioxide, it is possible to determine the development stage of the fruit and determine whether the electrochemical sensor module needs to be activated for detection. The advantage of this method over real-time monitoring of substances in the fruit is that the state of the sampled fruit will not be affected by long-term redox reactions and current transmission, ensuring that the fruit can accurately reflect the true state of the fruit of the entire plant, and the evaluation results of the fruit quality can provide guidance for picking, pruning and other links.

[0123] Furthermore, the model established by machine learning in the early planting of the corresponding plants can be combined to assess whether the fruit quality needs to be evaluated to activate the electrochemical sensor module for detection. During the whole process, the results of temperature sensing are used to correct the temperature of electrochemical sensors and gas sensors to help improve the detection accuracy.

[0124] The embodiments of the present invention can simultaneously monitor multiple parameters related to plant growth and provide comprehensive microenvironmental data, which can more accurately reflect the growth status and environmental conditions of plants and help to discover potential growth problems at an early stage.

[0125] The embodiment of the present invention adopts flexible materials and micro-nano processing technology to enable the sensor to be attached to the surface of the plant without affecting the normal growth of the plant.

[0126] The device provided by the embodiment of the present invention integrates a wireless communication module, which can transmit data to a mobile terminal in real time. Researchers can obtain plant growth data anytime and anywhere, thereby improving management efficiency.

[0127] The plant sensor in the device provided by the embodiment of the present invention can closely monitor the amount of gas in the microenvironment where the plant grows, the detected data is not easily diluted by the environment, and accurate sampling around the plant can be achieved.

[0128] The device provided in the embodiment of the present invention changes the ratio of graphene oxide wires and polyvinyl alcohol in the humidity sensing module so that the material has more excellent mechanical properties and humidity sensitivity characteristics.

[0129] The device provided in the embodiment of the present invention can enhance the conductivity and thermal stability of the material by changing the ratio of the temperature sensitive material 1400 polythiophene and graphene.

[0130] The device provided by the embodiment of the present invention uses the Pd / Ti3C2T in the flexible ethylene gas sensor 2110 x The mixing ratio of the composite material enables the composite material to produce the best response to ethylene gas.

[0131] The device provided by the embodiment of the present invention has a mixed ratio of zirconium oxide and graphene composite materials in the flexible carbon dioxide sensor, and the composite materials can improve the conductivity of zirconium oxide and the gas adsorption capacity.

[0132] The device provided by the embodiment of the present invention adopts the mixing ratio of tin oxide and graphene composite material in the flexible nitric oxide sensor, and this composite material can maximize the adsorption and detection effect of nitric oxide gas.

[0133] The device provided by the embodiment of the present invention uses a 3D printed microneedle 3200 in the electrochemical module, which can pierce the fruit skin to complete the detection while avoiding damage to the plant fruit to the greatest extent possible, and will not affect the normal growth and development of the plant.

[0134] The device provided in the embodiment of the present invention realizes efficient and accurate determination of sugar content, acidity and vitamin C content by directional immobilization of enzymes in the electrochemical module and optimization of immobilization conditions and DPV parameters.

[0135] The electrochemical sensor module 3000 of the device provided in the embodiment of the present invention uses a three-electrode system for measurement, and the rapid balancing action of the electrode 3500 enables the electrochemical reaction to quickly reach a steady state, shortening the response time. The three-electrode system can handle a wide range of target substances from low concentration to high concentration, improving the dynamic range of detection.

[0136] The working electrode 3400 in the electrochemical sensor module 3000 of the device provided in the embodiment of the present invention is modified with glucose oxidase, isocitrate dehydrogenase, and ascorbate oxidase to enhance the response to glucose, isocitrate, and vitamin C. The presence of the reference electrode 3300 and the counter electrode 3500 makes the modification of the working electrode 3400 more flexible, thereby improving the detection sensitivity.

[0137] The working electrode 3400 of the electrochemical sensor module 3000 of the device provided in the embodiment of the present invention has a His tag added to the N-terminus of the enzyme before enzyme fixation. Compared with other enzyme electrodes, this method can achieve a unified enzyme fixation direction, that is, the position of the His tag is used to guide the enzyme to be fixed on the electrode surface in a fixed direction. The significance of this is to make the side of the enzyme that is conducive to binding with the target face upward, making binding easier, thereby improving detection efficiency, and being more directional than general methods.

[0138] The electrochemical module of the device provided in the embodiment of the present invention can maximize the detection effect of the sugar content, acidity and vitamin C of plant fruits by changing the ratio of glucose oxidase, isocitrate dehydrogenase and ascorbic acid oxidase.

[0139] The device provided in the embodiment of the present invention uses a laser displacement sensor in the laser sensor module 4000, which can efficiently and accurately obtain information about the growth status of plants without destroying the plants.

[0140] The electrochemical sensor module 3000 in the device provided in the embodiment of the present invention is used to monitor the growth status of plant fruits, reflect the nutritional value and flavor quality of fruits in real time, and help guide the selection of varieties, fertilizer application and other tasks. However, the essence of electrochemical sensors is to detect substances in juice by applying current and quantify them by redox potential. Although the total amount of current is low, long-term monitoring will still cause damage to the fruit. In particular, long-term redox reactions will damage nutrients such as vitamin C and iron ions, causing premature decay or quality deterioration of the fruit. The final result is that the sampled fruit cannot correctly reflect the true situation of the fruit of the whole plant. Although controlling the number of detections and relaxing the detection interval can reduce this result to a certain extent, the sampling time point is relatively blind, and effective detection cannot be performed after the nutrient transport and accumulation process. To this end, the embodiment of the present invention uses multiple detection methods in coordination, and infers the growth state of the plant through the humidity of the leaves, the diameter of the stems and the content of key gas hormones. On the one hand, it can correctly reflect the growth state of the plant through cross-linking analysis of multiple factors (more accurate than single factors, because a single indicator of plants is often affected by multiple factors), and on the other hand, it can guide the sampling time of the electrochemical detection module, reduce the detection frequency, and reduce the impact on the fruit. Its detection logic includes: when the numerical value of the daily change of the stem diameter for nearly five days is plotted, and the slope of the fitting curve is greater than 3, it is considered that the plant has entered a rapid growth stage. At this time, the electrochemical sensor module 3000 is installed to avoid the impact caused by premature intervention. When the concentration of ethylene and the humidity above the leaves increase for 3 consecutive hours, and the concentration of carbon dioxide continues to decrease for 3 consecutive hours, an electrochemical module detection is performed, which can minimize the damage to the fruit as much as possible, and truly feedback whether the metabolic state of the fruit is good and whether the quality meets expectations.

[0141] The present invention provides a wearable multi-element collaborative plant growth monitoring device, comprising: a gas sensor module 2000, a laser sensor module 4000, an electrochemical sensor module 3000 and a central control module 5000; the gas sensor module 2000 is used to detect gas information of the surrounding environment of a target plant and send the gas information to the central control module 5000; wherein the gas information includes at least one of the following: ethylene gas concentration, carbon dioxide gas concentration or nitric oxide gas concentration; the laser sensor module 4000 is used to obtain the diameter of the stem of the target plant and send the diameter of the stem of the target plant to the central control module 5000; the central control module 5000 is used to generate an action signal if the gas information meets a preset condition and / or the diameter of the stem of the target plant meets a preset growth condition , sending the action signal to the electrochemical sensor module 3000; the electrochemical sensor module 3000 is used to receive the action signal, collect the juice of the target plant, obtain the biological information of the target plant based on the juice, and send the biological information to the central control module 5000; wherein the biological information includes at least one of the following: sugar content, acidity or vitamin C content; the diameter of the stem of the target plant is obtained by the laser sensor module 4000, and the biological information is collected by the electrochemical sensor module 3000 after the gas information and / or the stem diameter reaches the preset judgment condition, so that multiple modules can work together, focus on key detection nodes, improve the accuracy of plant growth monitoring and the effectiveness of detection results, and improve user experience. Improve the accuracy and efficiency of planting in agricultural work, and achieve improvements such as increased production, improved quality, and energy conservation.

[0142] Embodiment 2

[0143] On the basis of the above embodiments, an embodiment of the present invention provides a wearable multi-element collaborative plant growth monitoring system, comprising: an external communication device and the wearable multi-element collaborative plant growth monitoring device provided in the above embodiments.

[0144] The wearable multi-functional collaborative plant growth monitoring device sends data to external communication equipment through the central control module 5000, making it convenient for users to view the data.

[0145] Technicians in the relevant field can clearly understand that, for the convenience and conciseness of description, the specific working process of the wearable multi-element collaborative plant growth monitoring system described above can refer to the corresponding process in the aforementioned embodiment of the wearable multi-element collaborative plant growth monitoring device, and will not be repeated here.

[0146] The computer program product of the wearable multi-element collaborative plant growth monitoring device and system provided in the embodiments of the present invention includes a computer-readable storage medium storing program code. The instructions included in the program code can be used to execute the methods in the previous method embodiments. The specific implementation can be found in the method embodiments, which will not be repeated here.

[0147] Those skilled in the art can clearly understand that, for the convenience and brevity of description, the specific working process of the system and / or device described above can refer to the corresponding process in the aforementioned method embodiment, and will not be repeated here.

[0148] In addition, in the description of the embodiments of the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0149] If the functions are implemented in the form of software functional units and sold or used as independent products, they can be stored in a computer-readable storage medium. Based on this understanding, the technical solution of the present invention, or the part that contributes to the prior art, or the part of the technical solution, can be embodied in the form of a software product. The computer software product is stored in a storage medium, including several instructions to enable a computer device (which can be a personal computer, server, or network device, etc.) to perform all or part of the steps of the methods described in various embodiments of the present invention. The aforementioned storage medium includes: U disk, mobile hard disk, read-only memory (ROM, Read-Only Memory), random access memory (RAM, Random Access Memory), disk or optical disk, etc., which can store program codes.

[0150] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A wearable multi-element collaborative plant growth monitoring device, characterized in that: include: Gas sensor module, laser sensor module, electrochemical sensor module and central control module; The gas sensing module is used to detect gas information of the surrounding environment of the target plant and send the gas information to the central control module; wherein the gas information includes at least one of the following: ethylene gas concentration, carbon dioxide gas concentration or nitric oxide gas concentration; The laser sensor module is used to obtain the diameter of the stem of the target plant and send the diameter of the stem of the target plant to the central control module; The central control module is used to generate an action signal if the gas information meets a preset condition and / or the diameter of the stem of the target plant meets a preset growth condition, and send the action signal to the electrochemical sensor module; The electrochemical sensor module is used to receive the action signal, collect the juice of the target plant, obtain the biological information of the target plant based on the juice, and send the biological information to the central control module; wherein the biological information includes at least one of the following: sugar content, acidity or vitamin C content.

2. The wearable multi-element collaborative plant growth monitoring device according to claim 1, characterized in that: The gas sensing module comprises: a flexible gas substrate, a gas signal transmission processing unit and at least one gas sensor; The gas signal transmission processing unit and the gas sensor are arranged on the flexible gas substrate; The gas sensor is used to generate a gas electrical signal based on the concentration of the target gas, and send the gas electrical signal to the gas signal transmission processing unit; The gas signal transmission processing unit is used to amplify the gas electrical signal and transmit it to the central control module.

3. The wearable multi-element collaborative plant growth monitoring device according to claim 2, characterized in that: The gas sensor includes at least one of the following: a flexible ethylene gas sensor, a flexible carbon dioxide gas sensor or a flexible nitric oxide gas sensor.

4. The wearable multi-element collaborative plant growth monitoring device according to claim 1, characterized in that: The laser sensor module comprises: a laser transmitter and a laser receiver arranged opposite to each other and a laser data processing unit; The laser emitter is used to emit a laser beam; a portion of the laser beam is blocked by the stem of the target plant; The laser receiver is used to receive the laser beam; The laser data processing unit is used to determine the diameter of the stem of the target plant based on the laser beam received by the laser receiver.

5. The wearable multi-element collaborative plant growth monitoring device according to claim 4, characterized in that: The laser sensor module further includes: a first position adjustment mechanism and a second position adjustment mechanism; The laser emitter is arranged on the first position adjustment mechanism; The laser receiver is arranged on the second position adjustment mechanism; The first position adjustment mechanism is used to adjust the position of the laser emitter relative to the target plant; The second position adjustment mechanism is used to adjust the position of the laser receiver relative to the target plant.

6. The wearable multi-element collaborative plant growth monitoring device according to claim 1, characterized in that: The electrochemical sensing module comprises: an electrode substrate, a microneedle, a working electrode and a signal converter; The microneedle and the working electrode are both arranged on the electrode substrate; The microneedle is used to obtain the juice of the target plant; The working electrode is used to generate biological information of an electrical signal based on the juice and send the biological information to a signal converter; The signal converter converts the biological information of the electrical signal into the biological information of the digital signal, and sends the biological information of the digital signal to the central control module.

7. The wearable multi-element collaborative plant growth monitoring device according to claim 6, characterized in that: The diameter of the microneedle is 100-200 μm, and the length is 0.01 mm-0.05 mm.

8. The wearable multi-element collaborative plant growth monitoring device according to claim 6, characterized in that: A reaction enzyme is fixed on the working electrode; The reaction enzyme includes at least one of the following: glucose oxidase, isocitrate dehydrogenase or ascorbic acid oxidase.

9. The wearable multi-element collaborative plant growth monitoring device according to claim 6, characterized in that: The device also includes: a temperature and humidity sensing module; The temperature and humidity sensing module comprises: a fixed sheet, a camera, a variable length line, a humidity sensitive material and a temperature sensitive material; The fixed sheet is first connected to the humidity sensitive material through the variable length; the humidity sensitive material is connected to the temperature sensitive material; The fixing sheet is used to be fixedly connected to the leaves of the target plant; The camera is used to collect images of the humidity sensitive material and the temperature sensitive material.

10. A wearable multi-element collaborative plant growth monitoring system, characterized in that: include: An external communication device and a wearable multi-element collaborative plant growth monitoring device as described in any one of claims 1 to 9.

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