Oil-controlling, anti-inflammatory and soothing plant composition

Abstract

The present invention provides an oil-controlling, anti-inflammatory, and soothing botanical composition, comprising the following raw materials in parts by weight: 3-5 parts of Centella asiatica extract, 1-2 parts of Licorice extract, 2-4 parts of Herba Cynanchifoliae extract, 1-4 parts of Epilobium angustifolium extract, 2-5 parts of Trifolium repens flower extract, 4-6 parts of Lemongrass leaf extract, 7-9 parts of Rosa multiflora root extract, and 0.1-0.5 parts of a vitamin complex. The present invention utilizes reflux extraction, enzymatic hydrolysis, and fermentation techniques to fully extract the active substances in the root. The resulting Rosa multiflora root extract exhibits synergistic effects with the other raw materials, providing excellent oil-controlling, anti-inflammatory, and soothing effects on the skin.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetic preparations, and particularly relates to an oil-controlling, anti-inflammatory and soothing botanical composition. Background Art

[0002] With the accelerating pace of modern life and increasing environmental pollution, people face increasing stress. This stress not only impacts their mental health but also has a significant impact on their skin health. Oil secretion, in particular, has become a focus of much concern. Therefore, the research and development of cosmetics with oil-controlling, anti-inflammatory, and soothing properties has become a key research direction in the current cosmetics industry. Among the many active ingredients for acne treatment and oil control, natural plant extracts have attracted widespread attention due to their high biosafety and bioactivity.

[0003] However, maintaining the stability and effectiveness of active ingredients in plant extracts during their extraction and application remains a challenging technical challenge. Wild rose root extract, in particular, presents a complex composition and is challenging to extract. Therefore, employing scientifically effective extraction methods to improve extraction efficiency and the stability of active ingredients has become a key issue in cosmetics R&D. Summary of the Invention

[0004] The present invention provides an oil-controlling, anti-inflammatory and soothing plant composition, which has the effects of oil control, anti-inflammatory and soothing through natural ingredients extracted from plants.

[0005] To achieve the above objectives, the present invention provides an oil-controlling, anti-inflammatory, and soothing composition. This composition utilizes a specific extraction method, particularly an enzymatic fermentation extraction method for extracts from wild rose root, to improve extraction efficiency and the stability of the active ingredients. Furthermore, the present invention provides a leave-on cosmetic containing this oil-controlling, anti-inflammatory, and soothing composition. This cosmetic exhibits excellent oil-controlling, anti-inflammatory, and soothing effects, is safe and gentle to use, and is suitable for people of all skin types.

[0006] The present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides an oil-controlling, anti-inflammatory, and soothing composition comprising the following raw materials in parts by weight:

[0008] 3-5 parts of Centella asiatica extract;

[0009] 1-2 parts of licorice extract;

[0010] 2-4 parts of kidney tea extract;

[0011] 1-4 parts of Epilobium angustifolium extract;

[0012] 2-5 parts of red clover flower extract;

[0013] 4-6 parts of lemon bark leaf extract;

[0014] 7-9 parts of wild rose root extract;

[0015] Vitamin complex 0.1-0.5 parts;

[0016] The vitamin complex consists of beta-carotene, lutein, vitamin C and vitamin E, and the mass ratio of beta-carotene, lutein, vitamin C and vitamin E is 1:0.5-0.7:3-6:0.1-0.2.

[0017] In a second aspect, the present invention provides a method for preparing a Rosa multiflora root extract, comprising the following specific steps:

[0018] T1, drying the root of Rosa multiflora to constant weight, crushing it using a micro-airflow milling device to obtain A1, and sealing and storing it for later use;

[0019] T2. Reflux extract A1 with water-saturated ethyl acetate, and filter to obtain extract A2 and residue A3, wherein the mass ratio of water-saturated ethyl acetate to A1 is 20-50:1;

[0020] T3. Concentrate and freeze-dry A2 to obtain A4, which is then sealed, protected from light, and frozen for later use;

[0021] T4. Dry A3 at 79-85°C to constant weight, crush it to obtain A5, add deionized water twice the mass of A5, stir evenly to obtain a mixed solution, and enzymatically hydrolyze the mixed solution with cellulase, ligninase, and pectinase. After enzymatic hydrolysis for 2 hours, filter to obtain filtrate A6, wherein the mass ratio of cellulase, ligninase, and pectinase is 1:2:1-3, and the total mass of cellulase, ligninase, and pectinase is 8%-10% of the mass of A5;

[0022] T5. Ferment A6 with Rhodotorula glutinosus, and filter to obtain fermentation product A7, wherein the fermentation time is 48-72 hours, the initial fermentation pH is 5.5-6.5, the fermentation temperature is 25-35° C., and the amount of Rhodotorula glutinosus added is more than 10% of the mass of A6;

[0023] T6, ultrasonically disrupting the fermentation product A7 for 10 minutes, filtering and sterilizing to obtain A8;

[0024] T7. Mix A4 and A8 to obtain Rosa multiflora root extract A9.

[0025] In a third aspect, the present invention further provides a leave-on cosmetic comprising the following components in percentage by mass:

[0026] 1-8wt% oil-controlling, anti-inflammatory and soothing composition, 6-10wt% honey, 0.1-0.3wt% betaine, 0.1-1wt% sodium lauryl sulfate, 1-4wt% sodium hyaluronate, and the balance is deionized water.

[0027] The preparation method of leave-on cosmetics has the following specific steps:

[0028] X1: Add honey, betaine, and sodium lauryl sulfate to deionized water, heat to 60-65°C, and homogenize for 0.5 h;

[0029] X2: Cool down to 25-45°C, add the oil-controlling, anti-inflammatory and soothing composition and sodium hyaluronate, and homogenize for 2 hours to obtain the cosmetics.

[0030] The present invention innovatively compounds Centella asiatica extract, Glycyrrhiza uralensis extract, Camellia sinensis extract, Epilobium angustifolium extract, Trifolium pratense flower extract, Lemongrass leaf extract, and Rosa multiflorum root extract to produce an oil-controlling, anti-inflammatory, and soothing composition. The Rosa multiflorum root extract is prepared by first performing a reflux extraction with water-saturated ethyl acetate to extract the volatile components of flavonoids, phenols, and esters in the Rosa multiflorum root extract. Cellulase, ligninase, and pectinase are then used to enzymatically hydrolyze the reflux extraction residue to convert cellulose into sugars, thereby providing a carbon source for subsequent fermentation. The enzymatic hydrolysate is then fermented using red yeast to obtain red yeast metabolites, and finally the reflux extract is mixed with the fermentation metabolites to obtain wild rose root extract, which retains the active substances in the wild rose root as much as possible. At the same time, red yeast metabolites are also added. Experimental verification shows that the obtained wild rose root extract can be effectively enhanced in its antibacterial, soothing, oil-control, and acne-removing effects when compounded with Centella asiatica extract, galangal extract, kidney tea extract, angustifolia extract, red clover flower extract, and lemon bark leaf extract, showing a synergistic effect.

[0031] Compared with the prior art, the present invention has the following beneficial effects: the present invention uses a technique of first reflux extraction and then enzymatic hydrolysis and fermentation to extract wild rose roots, which not only retains the activity of easily oxidized and volatile substances, but also obtains polysaccharide compounds through enzymatic hydrolysis, and also obtains biological fermentation products, yeast metabolites and polysaccharides through fermentation. It can be seen that by adopting the extraction process of the present invention, it is possible to effectively retain the original active substances of wild rose roots and introduce more other natural active substances, thereby effectively improving the natural activity of wild rose root extract. In addition, the present invention combines the prepared wild rose root extract with Centella asiatica extract, licorice extract, kidney tea extract, angustifolia extract, red clover flower extract, lemon bark leaf extract, and vitamin complex, which has a synergistic effect and provides excellent oil control, anti-inflammatory, and soothing effects for the skin. DETAILED DESCRIPTION

[0032] In order to better understand the present invention, the present invention is further described below in conjunction with specific examples. The terms used in the examples are for describing specific embodiments and do not constitute a limitation on the scope of protection of the present invention.

[0033] The experimental methods in the following examples, where specific conditions are not specified, are generally based on conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, percentages and parts are calculated by weight.

[0034] Some of the raw materials and their sources are as follows:

[0035] Centella asiatica extract: purchased from Shaanxi New Horizon Biotechnology Co., Ltd.

[0036] Licorice extract: purchased from Fufeng Sinuote Biotechnology Co., Ltd.

[0037] Kidney tea extract: purchased from Fufeng Sinuote Biotechnology Co., Ltd.;

[0038] Epilobium angustifolium extract: purchased from Fufeng Sinuote Biotechnology Co., Ltd.;

[0039] Trifolium pratense flower extract: purchased from Fufeng Sinuote Biotechnology Co., Ltd.;

[0040] Lemon Bahao leaf extract: purchased from Kangluoxin (Guangdong) Biotechnology Co., Ltd.

[0041] Wild rose root: Hongsheng Professional Chinese Medicinal Herb Planting Cooperative, Qiaocheng District, Bozhou City;

[0042] Wild rose flower: Bozhou Bailintang Pharmaceutical Co., Ltd.

[0043] Cellulase: purchased from Ningbo Testo Biotechnology Co., Ltd., model: TSENZ1548, 500,000 U / g;

[0044] Ligninase: purchased from Hebei Hongtao Bioengineering Co., Ltd., model: 80498-15-3, 40 U / mg;

[0045] Pectinase: purchased from Ningbo Testo Biotechnology Co., Ltd., model: TSENZ1027, 40 U / mg;

[0046] Rhodotorula mucilaginosa: purchased from Ningbo Testo Biotechnology Co., Ltd., model: TS357009;

[0047] Yeast was purchased from Ningbo Testo Biotechnology Co., Ltd., model: TS372626.

[0048] Preparation of Rosa multiflora root extract:

[0049] Example 1:

[0050] T1, drying the root of Rosa multiflora to constant weight, crushing it using a micro-airflow milling device to obtain A1, and sealing and storing it for later use;

[0051] T2, reflux extraction of A1 with water-saturated ethyl acetate, filtering to obtain extract A2 and residue A3, wherein the reflux extraction temperature is 50°C, the reflux extraction time is 1.5 h, and the mass ratio of water-saturated ethyl acetate to A1 is 40:1;

[0052] T3. Concentrate and freeze-dry A2 to obtain A4, which is then sealed, protected from light, and frozen for later use;

[0053] T4. Dry A3 at 82°C to constant weight, crush it to obtain A5, add deionized water twice the mass of A5, stir evenly to obtain a mixed solution, and enzymatically hydrolyze the mixed solution with cellulase, ligninase, and pectinase. After enzymatic hydrolysis for 2 h, filter to obtain filtrate A6, wherein the mass ratio of cellulase, ligninase, and pectinase is 1:2:2, and the total mass of cellulase, ligninase, and pectinase is 9% of the mass of A5;

[0054] T5. Ferment A6 with Rhodotorula glutinosus, and filter to obtain fermentation product A7, wherein the fermentation time is 60 h, the initial fermentation pH is 5.8, the fermentation temperature is 30° C., and the amount of Rhodotorula glutinosus is 10% of the mass of A6;

[0055] T6, ultrasonically disrupting the fermentation product A7 for 10 minutes, filtering and sterilizing to obtain A8;

[0056] T7. Mix A4 and A8 to obtain Rosa multiflora root extract A9.

[0057] Example 2:

[0058] T1, drying the root of Rosa multiflora to constant weight, crushing it using a micro-airflow milling device to obtain A1, and sealing and storing it for later use;

[0059] T2, reflux extraction of A1 with water-saturated ethyl acetate, filtering to obtain extract A2 and residue A3, wherein the reflux extraction temperature is 50°C, the reflux extraction time is 1.5 h, and the mass ratio of water-saturated ethyl acetate to A1 is 40:1;

[0060] T3. Concentrate and freeze-dry A2 to obtain A4, which is then sealed, protected from light, and frozen for later use;

[0061] T4. Dry A3 at 85°C to constant weight, crush it to obtain A5, add deionized water twice the mass of A5, stir evenly to obtain a mixed solution, and enzymatically hydrolyze the mixed solution with cellulase, ligninase, and pectinase. After enzymatic hydrolysis for 2 h, filter to obtain filtrate A6, wherein the mass ratio of cellulase, ligninase, and pectinase is 1:2:1, and the total mass of cellulase, ligninase, and pectinase is 10% of the mass of A5;

[0062] T5. Ferment A6 with Rhodotorula glutinosus, and filter to obtain fermentation product A7, wherein the fermentation time is 48 h, the initial fermentation pH is 6.0, the fermentation temperature is 26° C., and the amount of Rhodotorula glutinosus is 10% of the mass of A6;

[0063] T6, ultrasonically disrupting the fermentation product A7 for 10 minutes, filtering and sterilizing to obtain A8;

[0064] T7. Mix A4 and A8 to obtain Rosa multiflora root extract A9.

[0065] Example 3:

[0066] T1, drying the root of Rosa multiflora to constant weight, crushing it using a micro-airflow milling device to obtain A1, and sealing and storing it for later use;

[0067] T2, reflux extraction of A1 with water-saturated ethyl acetate, filtering to obtain extract A2 and residue A3, wherein the reflux extraction temperature is 50°C, the reflux extraction time is 1.5 h, and the mass ratio of water-saturated ethyl acetate to A1 is 40:1;

[0068] T3. Concentrate and freeze-dry A2 to obtain A4, which is then sealed, protected from light, and frozen for later use;

[0069] T4. Dry A3 at 79°C to constant weight, crush it to obtain A5, add deionized water twice the mass of A5, stir evenly to obtain a mixed solution, and enzymatically hydrolyze the mixed solution with cellulase, ligninase, and pectinase. After enzymatic hydrolysis for 2 h, filter to obtain filtrate A6, wherein the mass ratio of cellulase, ligninase, and pectinase is 1:2:3, and the total mass of cellulase, ligninase, and pectinase is 8% of the mass of A5;

[0070] T5. Ferment A6 with Rhodotorula glutinosus, and filter to obtain fermentation product A7, wherein the fermentation time is 72 h, the initial fermentation pH is 6.3, the fermentation temperature is 33° C., and the amount of Rhodotorula glutinosus added is 10% of the mass of A6;

[0071] T6, ultrasonically disrupting the fermentation product A7 for 10 minutes, filtering and sterilizing to obtain A8;

[0072] T7. Mix A4 and A8 to obtain Rosa multiflora root extract A9.

[0073] Comparative Example 1: The wild rose roots in Example 1 were replaced with wild rose flowers, and the other steps and parameters were the same as those in Example 1.

[0074] Comparative Example 2: The mass ratio of cellulase, ligninase and pectinase in Example 1 was changed to 1:3:2, and the other steps and parameters were the same as those in Example 1.

[0075] Comparative Example 3: The red yeast rice in Example 1 was replaced with Saccharomyces paradoxus, and the other steps and parameters were the same as those in Example 1.

[0076] Comparative Example 4: The ultrasonic crushing step of T6 in Example 1 was deleted, and the other steps and parameters were the same as those in Example 1.

[0077] The raw materials and their weight ratios of an oil-controlling, anti-inflammatory and soothing composition are shown in Table 1.

[0078] Table 1 Raw materials and their mass ratios of the oil-controlling, anti-inflammatory and soothing composition

[0079]

[0080]

[0081]

[0082] Note: The preparation method of the wild rose root extract (alcohol extraction) in Table 1 is as follows: the raw material is A1 in Example 1, and A1 is reflux-extracted at 50°C for 1.5 h using 65 wt% ethanol. After the reflux extraction, the extract is filtered, and the filtrate is freeze-dried to obtain the wild rose root extract (alcohol extraction), wherein the mass ratio of A1 to 65 wt% ethanol is 1:40.

[0083] The vitamin complex in Table 1 consists of β-carotene, lutein, vitamin C, and vitamin E, wherein the mass ratio of β-carotene, lutein, vitamin C, and vitamin E is 1:0.6:4:0.2.

[0084] The composition of a leave-on cosmetic is shown in Table 2, and the specific preparation steps are as follows:

[0085] X1: Add honey, betaine, and sodium lauryl sulfate to deionized water, heat to 63°C, and homogenize for 0.5 h;

[0086] X2: Cool down to 30° C., add the oil-controlling, anti-inflammatory and soothing composition and sodium hyaluronate, and homogenize for 2 hours to obtain the cosmetics.

[0087] Table 2 Components and mass percentages of leave-on cosmetics

[0088]

[0089] Safety trials

[0090] Using the 2015 "Technical Specifications for Safety of Cosmetics" as a reference standard, the cosmetics corresponding to Examples 1-7 were evaluated for irritation using a skin patch test on 70 randomly distributed individuals aged 16-65 years.

[0091] Test Method: Place the test substance in a patch tester at a dosage of 0.020-0.025g. Cover the patch tester with non-irritating cloth-based tape on the subject's back or forearm. Use the palm of your hand to gently press the patch tester to evenly adhere to the skin surface. Continue for 24 hours. Remove the patch tester and observe the skin reaction 30 minutes after the indentation disappears. If the result is negative, observe again 24 hours and 48 hours after the patch test.

[0092] Evaluation criteria:

[0093] Grade 0: negative reaction;

[0094] Grade 1: Suspicious reaction, only slight erythema;

[0095] Grade 2: weak positive reaction, erythema, infiltration, edema, and papules may be present;

[0096] Grade 3: Strong positive reaction, with erythema, infiltration, edema, papules, and the reaction may extend beyond the test area;

[0097] Grade 4: Very strong positive reaction, obvious erythema, severe infiltration, edema, confluent blister, and reaction beyond the test area.

[0098] Test results: All subjects had negative skin reactions.

[0099] The above test results show that the cosmetics containing the oil-controlling, anti-inflammatory and soothing composition provided by the present invention are mild and non-irritating to the skin and are safe to use.

[0100] Propionibacterium acnes inhibition test

[0101] With reference to WS / T650-2019, the inhibition ring test was used. A 24-hour fresh slant culture of P. acnes was washed with PBS and diluted to 5.0×10 5 CFU / mL-5.0×10 6 CFU / mL is reserved.

[0102] Preparation of antibacterial sheets: Cut filter paper sheets with a diameter of 5 mm and a thickness not exceeding 4 mm, and soak each group of 4 sheets in 10 mL of a sterile aqueous solution of compositions 1-5 and compositions ①-⑨ with a concentration of 1 mg / L.

[0103] Preparation of negative control samples: Take samples of the same material, make discs (blocks) of the same size as the test group, and soak them in sterile water.

[0104] A sterile cotton swab was dipped in a solution of 5.0×10 5 CFU / mL-5.0×10 6 Apply CFU / mL of P. acnes suspension evenly three times on the surface of a suitable culture medium plate. Each time you apply it, the plate should be rotated 60°, and finally the cotton swab should be applied around the edge of the plate. Cover the plate and let it dry at room temperature for 5 minutes. Place one infected plate for each test, with four test samples and one negative control sample on each plate, for a total of five samples. Use sterile tweezers to take the sample and place it on the surface of the plate. The negative control sample should be placed in the center of the plate, and the test sample should be placed on the four sides. After placing it, use sterile tweezers to gently press the sample to make it stick to the surface of the plate. The centers of each sample should be at least 25 mm apart, and at least 15 mm away from the edge of the plate. Cover the plate, incubate at 36℃±1℃ for 16-18 hours, and observe the results. Repeat the test three times. Use a vernier caliper to measure the diameter of the antibacterial ring (including the patch) and record it. When measuring the antibacterial ring, a uniform and completely sterile antibacterial ring should be selected, and the diameter should be measured based on the outer edge of the antibacterial ring.

[0105] The negative control sample should produce no inhibition ring. If the diameter of the inhibition ring on the test sample is greater than 7 mm, it is considered to have an antibacterial effect; if the diameter of the inhibition ring is ≤ 7 mm, it is considered to have no antibacterial effect. The test is considered qualified if the inhibition effect is observed in all three repeated tests. See Table 3 for detailed results.

[0106] Table 3 Pseudomonas acnes inhibition test

[0107] experimental group Inhibition zone size / mm Composition 1 19.82 Composition 2 21.22 Composition 3 17.84 Composition 4 15.73 Composition 5 15.43 Composition ① 15.52 Composition ② 14.34 Composition ③ 15.17 Composition ④ 13.75 Composition ⑤ 11.18 Composition ⑥ 7.83 Composition ⑦ 3.04 Composition ⑧ 12.22 Composition ⑨ 14.33

[0108] The results show that in terms of antibacterial performance, compositions 1-5 are superior to compositions ①-⑨, and there is a certain synergistic effect between the components in the specified ratios of the present invention.

[0109] Detection of lipid accumulation in the cytoplasm of SZ95 cells by Nile red staining

[0110] (1) Cell inoculation: SZ95 cells in good growth condition were collected, digested with trypsin, and centrifuged. The cells were resuspended in DMEM complete medium and the cell concentration was adjusted to 3×10 5 Sterile cell slides were added to 12-well plates, and 2 mL of cell suspension was evenly seeded into each well. The plates were then placed in a 37°C, 5% CO2 incubator for incubation.

[0111] (2) After the cells adhered to the wall, discard the old culture medium and wash twice with PBS. Testosterone (6ug / ml) and linoleic acid (100μM) were used to induce lipid synthesis. A model group (no sample added) and a sample group (2μL, 1mg / L of composition 1-5 and composition ①-⑨ added) were set up respectively, and then placed in a 37°C, 5% CO2 incubator for further incubation for 24h;

[0112] (3) After incubation for 24 hours, discard the culture medium, wash twice with cold PBS, and fix with 4% paraformaldehyde at room temperature for 10 minutes. After fixation, stain with Nile red stain (100 μg / mL) and incubate in a 37°C incubator for 10 minutes. Aspirate the stain and then wash with PBS 3-5 times. Remove the cell slide and place it on a slide. Observe the staining under a fluorescence microscope. Cells with strong orange-red fluorescence are lipid-rich positive cells.

[0113] (4) The collected photos were analyzed for fluorescence intensity using ImageJ software.

[0114] SPSS 19.0 statistical software package was used for analysis. The results are shown in Table 4.

[0115] Table 4 Lipid accumulation test results

[0116] experimental group Cumulative degree / % Composition 1 27.35 Composition 2 27.32 Composition 3 29.17 Composition 4 31.27 Composition 5 32.34 Composition ① 39.11 Composition ② 34.25 Composition ③ 49.38 Composition ④ 83.17 Composition ⑤ 72.26 Composition ⑥ 88.81 Composition ⑦ 84.27 Composition ⑧ 73.25 Composition ⑨ 35.66 Model Group 100

[0117] Result analysis: From the results of compositions 1-5, it can be seen that the oil-controlling, anti-inflammatory and soothing composition provided by the present invention has good oil-control efficacy, especially compositions 1-2. Therefore, for cost-effectiveness considerations, composition 2 was selected as the optimal embodiment.

[0118] From the results of composition 2 and compositions ①-④, it can be seen that the composition containing the wild rose root extract extracted by the preparation method of the present invention has a good oil control effect, and any change in the process steps defined in the present invention cannot achieve the effect achieved by the present invention.

[0119] From the results of composition 2 and compositions ⑤-⑧, it can be seen that the raw material components in the oil-controlling, anti-inflammatory and soothing composition provided by the present invention have a certain synergistic effect, and the effect achieved by the present invention cannot be achieved by omitting or replacing any one of them.

[0120] From the results of composition 2 and composition 9, it can be seen that the wild rose root extract prepared by the present invention has a better oil-control effect than the traditional alcohol-extracted wild rose root extract.

[0121] Human efficacy trials

[0122] Oil control efficacy measurement

[0123] The oil-control efficacy of leave-on cosmetics corresponding to use cases 1-7 and blank application cases was evaluated using the T / ZHCA002-2018 cosmetics oil-control efficacy test method. 80 randomly distributed people aged 16-45 years were tested.

[0124] Test method:

[0125] Preliminary Preparation: The test site is the forehead. The sample application area and control area are randomly distributed on the left and right sides of the forehead. The sample application area and the control area are equal in size, each area is at least 3cm x 3cm, and the area spacing is at least 1cm to ensure statistical balance between the sample application area and the control area.

[0126] Before the test, the subjects sat quietly in a standard test environment for at least 20 minutes, were not allowed to drink water or beverages, had their foreheads exposed, remained relaxed, and avoided touching the tested area.

[0127] Testing: Follow the instructions for the skin surface sebum tester. Under the supervision of the tester, the subject cleansed the test area with an alkaline soap-based cleanser, rinsed thoroughly with water, and blotted dry with a non-stick absorbent tissue. Within 3 minutes, the sebum content of the sample application area and the control area was measured three times at different locations within each area. The average of these three measurements was used as the initial value.

[0128] Sample is at (2.0 ± 0.1) mg / cm 2 Apply the sample once, take quantitative samples by syringe or equivalent, apply the sample evenly on the specified area using a latex finger cot, and record the actual amount of sample applied.

[0129] At the set measurement time point, measurements were taken three times at different locations in the sample application area and the control area to obtain the amount of sebum on the skin surface of each area. The measurement results were tabulated as the average value of the three measurements.

[0130] The interval between the set test time points should be no less than 1 hour. Multiple test time points can be set according to evaluation needs. The entire test cycle usually does not exceed 24 hours.

[0131] When measuring at different measurement time points, avoid locations that have already been measured.

[0132] During the test, the subject's forehead was exposed and touching the tested area was avoided.

[0133] Cosmetic testing must be completed by the same tester using the same instrument for the same subject. If a subject experiences an adverse skin reaction during sample use, the test must be terminated and the subject must receive appropriate medical treatment. Adverse reactions must be recorded.

[0134] Results: The measured values ​​of the sample smear area and the control area were compared and statistically analyzed. The specific data are shown in Table 5.

[0135] formula: Where A0 is the mean value of sebum volume test in the control area, and A is the mean value of sebum volume test in the test area.

[0136] Table 5 Oil control efficacy test results

[0137] experimental group Oil output suppression rate Application Example 1 65.24％ Application Example 2 52.27％ Application Example 3 31.56％ Application Example 4 63.72％ Application Example 5 62.26％ Application Example 6 61.22％ Application Example 7 62.75％ Blank Application Example 9.33％

[0138] Result analysis: From the results of Application Examples 1-7, it can be seen that the oil-controlling, anti-inflammatory and soothing composition provided by the embodiments of the present invention has good oil-control effect, indicating that the use of the addition amount defined in the present invention can better exert its oil-control effect.

[0139] Soothing efficacy test

[0140] This test verifies the soothing effect of leave-on cosmetics in Application Examples 1-7 and a blank application example on damaged skin.

[0141] Specifically, 80 volunteers were recruited and divided into 8 groups of 10 people each, with half male and half female, aged 20-35 years old. The test method is as follows:

[0142] (1) Adhesive tape irritation test: The adhesive tape was repeatedly applied to the inner skin of the forearm 15 times to damage the skin;

[0143] (2) Each tester applied 0.25 g of the left hand for application examples 1-7 and the blank application example;

[0144] (3) Observe and evaluate the results after 15 minutes.

[0145] Scoring: A 5-point system was used, with 1 point - redness became more severe; 2 points - no improvement; 3 points - slight improvement, but obvious redness was still visible; 4 points - redness almost disappeared; 5 points - redness completely disappeared. The results are shown in Table 6.

[0146] Table 6 Soothing effect test results

[0147] experimental group Average rating for soothing Application Example 1 4.9 Application Example 2 4.9 Application Example 3 4.8 Application Example 4 4.6 Application Example 5 4.7 Application Example 6 4.6 Application Example 7 4.5 Blank Application Example 2.4

[0148] Human patch test

[0149] Histamine was used as a stimulus to evaluate the effect of the samples on improving symptoms such as erythema and itching of histamine-sensitized skin.

[0150] Test samples: Application Examples 1-7 and blank application examples.

[0151] Volunteers and test environment: 8 volunteers with normal skin, aged 20-30 years, of random gender, were selected; the test environment was 25±2℃ and humidity 60%RH.

[0152] Test method: Wash the inner arm of the subject with clean water and blow dry with cold air for 15-20 minutes; apply 20μL of 1% histamine solution on a patch sticker and stick it to the inner arm of the volunteer. Remove the sticker after 30 minutes, mark the patch position with a marker, and apply different test samples. Record the time it takes for the erythema and pain and itching at the patch position to subside.

[0153] The test results are shown in Table 7.

[0154]

[0155]

[0156] As shown in Tables 6-7, Application Examples 1-7 have significant anti-inflammatory and soothing effects compared to the blank application example.

[0157] The "compositions," "application examples," and "embodiments" described above are some, but not all, of the embodiments of this application. The detailed description of the embodiments of this application is not intended to limit the scope of the claimed application but merely represents selected embodiments of this application. All other embodiments derived by those skilled in the art based on the embodiments of this application without inventive effort are also within the scope of protection of this application.

Claims

1. An oil-controlling, anti-inflammatory and soothing composition, characterized in that: The oil-controlling, anti-inflammatory and soothing composition comprises the following raw materials in parts by weight: 3-5 parts of Centella asiatica extract; 1-2 parts of licorice extract; 2-4 parts of kidney tea extract; 1-4 parts of Epilobium angustifolium extract; 2-5 parts of red clover flower extract; 4-6 parts of lemon bark leaf extract; 7-9 parts of wild rose root extract; Vitamin complex 0.1-0.5 parts; The vitamin complex consists of β-carotene, lutein, vitamin C, and vitamin E, and the mass ratio of β-carotene, lutein, vitamin C, and vitamin E is 1:0.5-0.7:3-6:0.1-0.2; The method for extracting the wild rose root extract is specifically enzymatic fermentation extraction, and the steps are as follows: T1, drying and crushing the root of wild rose to obtain A1; T2. Reflux extract A1 with water-saturated ethyl acetate, and filter to obtain extract A2 and residue A3, wherein the mass ratio of water-saturated ethyl acetate to A1 is 20-50:1; T3. Concentrate and freeze-dry A2 to obtain A4, which is then sealed, protected from light, and frozen for later use; T4. Dry A3 at 79-85°C to constant weight, crush it to obtain A5, add water twice the mass of A5, stir evenly to obtain a mixed solution, and enzymatically hydrolyze the mixed solution with cellulase, ligninase, and pectinase. After enzymatic hydrolysis for 2 hours, filter to obtain filtrate A6, wherein the mass ratio of cellulase, ligninase, and pectinase is 1:2:1-3, and the total mass of cellulase, ligninase, and pectinase is 8%-10% of the mass of A5; T5. Ferment A6 with Rhodotorula glutinosus, and filter to obtain fermentation product A7, wherein the fermentation time is 48-72 hours, the initial fermentation pH is 5.5-6.5, the fermentation temperature is 25-35° C., and the amount of Rhodotorula glutinosus added is more than 10% of the mass of A6; T6, ultrasonically disrupting the fermentation product A7 for 10 minutes, filtering and sterilizing to obtain A8; T7. Mix A4 and A8 to obtain Rosa multiflora root extract A9.

2. The oil-controlling, anti-inflammatory and soothing composition according to claim 1, characterized in that: The oil-controlling, anti-inflammatory and soothing composition comprises the following raw materials in parts by weight: 3-4 parts of Centella asiatica extract; 1-2 parts of licorice extract; 2-3 parts of kidney tea extract; 1-3 parts of Epilobium angustifolium extract; 2-4 parts of red clover flower extract; 4-5 parts of lemon bark leaf extract; 7-8 parts of wild rose root extract; Vitamin complex 0.3-0.5 parts.

3. The oil-controlling, anti-inflammatory and soothing composition according to claim 1, characterized in that: The oil-controlling, anti-inflammatory and soothing composition comprises the following raw materials in parts by weight: 4 parts of Centella asiatica extract; 1 part of licorice extract; 3 parts of kidney tea extract; 3 parts of Epilobium angustifolium extract; 4 parts of red clover flower extract; 5 parts of lemon bark leaf extract; 8 parts of Rosa multiflora root extract; Vitamin complex 0.3 parts.

4. A leave-on cosmetic, characterized in that: The invention comprises the oil-controlling, anti-inflammatory and soothing composition according to any one of claims 1 to 3.

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