A solution proportioning mechanism and solution dilution device

Abstract

The present invention discloses a solution proportioning mechanism and a solution dilution device in the technical field of solution proportioning and dilution, aiming to solve the problem in the prior art that the manual dilution of proportioning solutions is cumbersome and easy to cause pollution. The mechanism comprises a piston cylinder and a piston assembly arranged in the piston cylinder; the piston assembly comprises a bottom plate and a partition plate arranged on the bottom plate, the bottom plate matches the inner cavity cross section of the piston cylinder, the bottom plate is used to divide the interior of the piston cylinder into an upper cavity and a lower mixing cavity, the partition plate is used to divide the upper cavity into a plurality of mutually unconnected liquid supply cavities, the partition plate can slide through the upper half of the piston cylinder and keep each liquid supply cavity sealed; the present invention is used to simplify the proportioning work of the solution in the test, and the required concentration can be quickly obtained without repeated weighing, the operation method is simple and convenient, and a large number of test liquid samples of different concentrations can be easily and quickly obtained, and the liquid preparation process does not contact the external environment, which is conducive to ensuring accurate experimental results.

Description

Technical Field

[0001] The invention relates to a solution proportioning mechanism and a solution diluting device, belonging to the technical field of solution proportioning and dilution. Background Art

[0002] In the field of chemical engineering and biological experimental technology, it is often necessary to mix and dilute the solution components to ensure that the mixed reactants react completely or to mix them into the required use state. For example, in the bacterial reverse mutation test of liquid medical devices for human assisted reproductive technology, the blastocyst culture solution needs to be diluted with physiological saline to four concentrations of 50%, 25%, 12.5%, and 5% (i.e., 5μL / dish) respectively. In the preparation process of culture solutions of multiple concentrations, each required concentration is usually separately proportioned by step-by-step weighing. In the preparation process, it is necessary to repeatedly aspirate or supply liquid to make the proportioned solution reach the required concentration. After use, the container needs to be cleaned and dried for re-weighing and proportioning. First of all, this continuous weighing and proportioning method is relatively cumbersome, and repeated contact of the test liquid with the external environment in the external environment is likely to cause contamination of the experimental liquid, which is likely to affect the test results. Due to the cumbersome operation of the proportioning step, it also affects the exploration of the optimal concentration range of various tests. Summary of the invention

[0003] The purpose of the present invention is to overcome the deficiencies in the prior art and provide a solution proportioning mechanism and a solution dilution device, which are used to simplify the proportioning work of solutions in experiments. The required concentration can be quickly obtained without repeated weighing. The operation method is simple and convenient, and a large number of test liquid samples of different concentrations can be easily and quickly obtained. The liquid preparation process does not come into contact with the external environment, which is conducive to ensuring accurate experimental results.

[0004] To achieve the above object, the present invention is implemented by adopting the following technical solutions:

[0005] The present invention provides a solution proportioning mechanism, comprising a piston cylinder, a piston assembly disposed in the piston cylinder, a first driving module, and a second driving module for driving the piston assembly to move along the axis direction of the piston cylinder;

[0006] The piston assembly includes a bottom plate and a partition plate arranged on the bottom plate. The bottom plate matches the inner cavity cross-section of the piston cylinder. The bottom plate is used to divide the interior of the piston cylinder into an upper cavity and a lower mixing cavity. The partition plate is used to divide the upper cavity into a plurality of mutually unconnected liquid supply cavities. The partition plate can slide through the upper half of the piston cylinder and maintain the sealing of each liquid supply cavity. The bottom plate is provided with an opening for connecting each liquid supply cavity to the lower mixing cavity.

[0007] The bottom of the piston cylinder is provided with sealing holes corresponding to the positions of the openings. The sealing holes have the same inner diameter as the openings. A blocking rod is slidably provided on each sealing hole. The first driving module is used to drive each blocking rod to move along the axial direction of the sealing hole and to block the openings provided on the bottom plate.

[0008] A liquid supply portion is provided at the top of the piston cylinder, and the liquid supply portion is used to supply different types of liquids to be mixed to each liquid supply cavity, and a liquid outlet portion is provided at the bottom of the piston cylinder.

[0009] Specifically, the cavity cross-section of the piston cylinder is polygonal, any side of the partition plate can abut against the inner wall of the piston cylinder, and the top of the piston cylinder is provided with a special-shaped opening matching the shape of the partition plate. The partition plate can pass through the special-shaped opening and be in sliding and sealing contact with the piston cylinder.

[0010] Specifically, the second driving module is a servo linear motion module, and a mounting block is fixedly provided at the output end of the servo linear motion module. The movable direction of the mounting block is parallel to the axial direction of the piston cylinder. A pressure block is rotatably provided on the mounting block. The pressure block includes two pressure edges whose end points are connected to each other. The connection point positions of the two pressure edges are rotatably provided on the mounting block, and the bottom ends of the two pressure edges are respectively pressed on both sides of the partition plate.

[0011] Specifically, movable plates are installed on the top of both sides of the partition plate, each movable plate is provided with a movable opening, and action rods are installed on the bottom ends of the two pressure edges, and each action rod is movably arranged within the limited range of the movable opening.

[0012] Specifically, the piston cylinder includes an upper connecting part and a lower connecting part that can be assembled with each other. The upper connecting part and the lower connecting part are both provided with guide grooves whose positions match each other. The side of the partition plate is provided with guide ribs that can match the shape of the guide grooves. The upper connecting part and the lower connecting part are both provided with observation windows, and the side of the observation window is provided with display scales.

[0013] A solution dilution device provided by the present invention comprises any of the solution proportioning mechanisms described above, and also comprises a pipette and a third driving module for driving the pipette to move within a spatial range. The pipette is provided with a liquid inlet pipe connected to a liquid outlet of a piston cylinder and an air inlet pipe for connecting to an external pressure gas. Both the liquid inlet pipe and the air inlet pipe can control the on-off of their passages. A three-way solenoid valve is installed at the liquid outlet of the piston cylinder. The other two ports of the three-way solenoid valve are respectively connected to the liquid inlet pipe and the liquid outlet pipe. The liquid outlet pipe is used to discharge reflux liquid from the liquid inlet pipe.

[0014] Specifically, it also includes a liquid supply box, which is used to separate and store different types of solutions, and the bottom of each solution storage chamber is provided with a connecting pipe connected to the corresponding liquid supply cavity.

[0015] Specifically, a liquid supply port is provided on the top of the liquid supply box, a box cover is installed at the position of the liquid supply port, an opening is provided on the box cover, and a filter layer is provided at the position of the opening.

[0016] Specifically, it also includes a 96-well plate and a waste liquid collection box. The third driving module can at least execute the action program of the pipette according to the 96-well plate injection method. The waste liquid collection box is used to temporarily store the waste liquid from the pipette.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] The present invention forms a constant-volume mixing cavity below the piston cylinder through special design of the piston cylinder and the piston assembly, and then controls the blocking rod to realize the supply of the corresponding supply solution, that is, a fixed amount of liquid can be taken out of the mixing cavity at one time for mixing and preparing without weighing. The volume of the mixing cavity can be controlled by the through hole to control the concentration ratio of the solution, and the solution can be configured in a manner of preparing in the order of concentration gradient, which can greatly save the time spent in the preparation process. In addition, the mixing process is automatically carried out in a closed environment, and various liquids have no obvious contact with the outside world, which reduces the possibility of liquid contamination. For experimental operations, it is less likely to affect the test results. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 is a schematic diagram of the overall structure of a solution dilution device provided in an embodiment of the present invention;

[0020] Figure 2 The present invention Figure 1 An enlarged view of the structure at position A of the solution dilution device provided in the embodiment;

[0021] Figure 3 The present invention Figure 1 An enlarged view of the structure at position B of the solution dilution device provided in the embodiment;

[0022] Figure 4 is a front view of a solution dilution device provided in an embodiment of the present invention;

[0023] Figure 5 The present invention Figure 4 A CC-direction cross-sectional view of a solution dilution device provided in an embodiment;

[0024] Figure 6 is a top view of a solution dilution device provided in an embodiment of the present invention;

[0025] Figure 7 The present invention Figure 6 A cross-sectional view of the solution dilution device provided in the embodiment in the DD direction;

[0026] Figure 8is a top cross-sectional schematic diagram provided by an embodiment of the present invention;

[0027] Figure numerals: 1. piston cylinder; 101. upper connecting part; 102. lower connecting part; 103. observation window; 2. piston assembly; 201. bottom plate; 202. partition plate; 3. first drive module; 4. second drive module; 401. servo linear motion module; 402. mounting block; 403. pressure block; 5. blocking rod; 6. movable sheet; 7. action rod; 8. pipette; 9. third drive module; 10. liquid inlet pipe; 11. air inlet pipe; 12. three-way solenoid valve; 13. liquid outlet pipe; 14. liquid supply box; 15. connecting pipe; 16. box cover; 17. filter layer; 18. 96-well plate; 19. waste liquid collection box. DETAILED DESCRIPTION

[0028] The present invention will be further described below in conjunction with the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the present invention, and cannot be used to limit the protection scope of the present invention.

[0029] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and the like are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, features defined as "first", "second", and the like may explicitly or implicitly include one or more of the features. In the description of the present invention, unless otherwise specified, "multiple" means two or more.

[0030] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood by specific circumstances. Embodiment 1

[0031] A solution proportioning mechanism provided in an embodiment of the present invention is used to simplify the proportioning work of solutions in experiments. The required concentration can be quickly obtained without repeated weighing. The operation method is simple and convenient, and a large number of test liquid samples with different concentrations can be easily and quickly obtained. In addition, the liquid preparation process does not contact the external environment, which is conducive to ensuring accurate experimental results. In order to realize the structural function of the mechanism, the device is provided here, including a piston cylinder 1, a piston assembly 2 arranged in the piston cylinder 1, a first driving module 3, and a second driving module 4 for driving the piston assembly 2 to move along the axial direction of the piston cylinder 1; Figure 1 as well as Figure 7 As shown, the piston assembly 2 includes a bottom plate 201 and a partition plate 202 disposed on the bottom plate 201, wherein the bottom plate 201 matches the inner cavity cross-section of the piston cylinder 1, the bottom plate 201 is used to divide the interior of the piston cylinder 1 into an upper cavity and a lower mixing cavity, and the partition plate 202 is used to divide the upper cavity into a plurality of liquid supply cavities that are not connected to each other, such as Figure 1 As shown, the partition plate 202 can slide through the upper half of the piston cylinder 1 and maintain the sealing of each liquid supply cavity. The bottom plate 201 is provided with openings for connecting each liquid supply cavity to the lower mixing cavity. These openings are used to open when mixing solutions, so that the corresponding liquid supply cavity can flow into the mixing cavity below; and in order to achieve the closure of these openings, sealing holes corresponding to the positions of each opening are opened at the bottom of the piston cylinder 1, and the sealing holes are set to have the same inner diameter as the openings. A blocking rod 5 is slidably arranged on each sealing hole, and a first driving module 3 is provided to drive each blocking rod 5 to move along the axial direction of the sealing hole and to block the openings opened on the bottom plate 201. The structure of blocking the openings is shown in FIG. Figure 7As shown, the plugging of the opening is achieved by inserting the blocking rod 5 through the hole, so as to prevent the upper liquid supply cavity from continuing to flow the solution into the lower mixing cavity; and in order to realize the supply and discharge of the original liquid, a liquid supply part is provided at the top of the piston cylinder 1, and the liquid supply part is used to supply different types of liquids to be mixed to each liquid supply cavity. The specific structural form is not limited here, and a liquid outlet is provided at the bottom of the piston cylinder 1, such as using a pipeline and a valve to discharge the liquid. Through the setting of the above mechanism, when the mechanism is used, if it is necessary to prepare the mixed liquid according to the corresponding concentration gradient, here, the bacterial reverse mutation test of liquid medical devices for human assisted reproductive technology is taken as an example (the required blastocyst culture liquid is diluted with physiological saline to four concentrations of 50%, 25%, 12.5%, and 5%, respectively), and a partition plate 202 is set to divide only the upper half of the piston cylinder 1 into two liquid supply cavities (according to different test requirements, multiple liquid supply cavities can also be set, which can be used to mix multiple different types of liquids. When multiple liquid supply cavities are used, the corresponding blocking rods 5 should also be multiple), one of which is a liquid supply cavity. The cavity is used to supply blastocyst culture fluid, and the other liquid supply cavity is used to supply physiological saline. According to the required concentration gradient, 100% blastocyst culture fluid is first configured here. In the initial state, the mixing cavity is in a liquid-free state. According to the required usage, the bottom plate 201 is first adjusted to the position of the required usage through a control mechanism (such as a servo motor, etc.) (preferably using an automated calculation method introduced by a control unit), and then the blocking rod 5 corresponding to the blastocyst culture fluid is pulled out (so that it leaks out of the liquid supply opening of the liquid supply cavity), allowing the blastocyst culture fluid to flow into the mixing chamber below. Since physiological saline is not needed at this time, the drawn blocking rod is removed after the supply is completed. The rod 5 is reset, and in the state of closing the culture fluid passage, the bottom plate 201 is controlled to squeeze the mixing cavity so that the liquid inside it flows out from the liquid outlet. When a 50% concentration culture fluid is needed, the bottom plate 201 can be moved up to empty a double volume according to the previous residual blastocyst culture fluid volume (here, the volume ratio and mass ratio are calculated as 1:1. In actual use, the required action volume of the bottom plate 201 can be changed according to the actual mass ratio), and then the blocking rod 5 corresponding to the opening of the chamber where the physiological saline is located is pulled out, so that the physiological saline can be mixed with the remaining blastocyst culture fluid in equal volume, thereby obtaining a 50% concentration. The blastocyst culture medium of the corresponding concentration is then reset, and the bottom plate 201 is driven to descend and squeeze the mixing cavity space to achieve the outflow of the culture medium of the corresponding concentration. According to this method, they can be configured one by one from high to low according to the concentration gradient. Only the position of the blocking rod 5 and the position of the bottom plate 201 need to be changed to quickly obtain and supply a variety of culture solutions of different concentrations. There is no need for manual participation in repeated weighing. The mixed solution does not contact the external environment and is not easily contaminated. It is conducive to the rapid preparation of solutions of various concentrations for testing, which greatly simplifies the cumbersomeness of the experimental steps brought about by manual weighing and is conducive to exploring the optimal concentration range of the experiment.In the above method, considering that bubbles are likely to exist under the bottom plate 201 in the piston cylinder 1, which may cause a large error in the actual concentration during the proportioning process, as a preferred embodiment, a sloped surface transitioning to the edge of the bottom plate 201 is provided at the position of each opening of the bottom plate 201, so that the bubbles can flow out through the opening position as much as possible, thereby avoiding the deviation between the actual volume of the inflowing liquid and the estimated volume due to the appearance of bubbles. During the implementation of the above method, although it is difficult for the liquid to interact with the turbulent flow after the bubbles in the lower mixing cavity are completely discharged, the change in the position of the blocking rod 5 may cause the local mixed liquid to affect the purity of the original liquid. Although the purity effect can be ignored in the short term, if the original liquid is not replaced for a long time, it may eventually affect the ratio of concentration. There are slight differences in changes. For more precise test requirements, it may affect the accuracy of the final test results. Therefore, it is not recommended to use it for a long time in an experimental environment with higher precision. At least the original liquid needs to be replaced regularly. In order to avoid the impact of the above problems, during operation, the top surface position of the blocking rod 5 should be as close to the bottom surface position of the bottom plate 201 as possible when it is pulled out, and the diameter of the liquid supply opening (including its sealing ring layer) of the bottom plate 201 should be controlled as much as possible within 3~4mm, so as to reduce the turbulent flow interaction between the upper and lower layers of the bottom plate 201 as much as possible. After the lower mixing chamber is filled, the blocking rod 5 should be sealed immediately to further reduce the impact on the purity of the original liquid. As another preferred embodiment, in order to avoid the inconvenience of subsequent mixing configuration due to incorrect concentration configuration (which may lead to various situations such as waste in configuration), a cleaning pipe can be provided below or at the bottom of the piston cylinder 1 to communicate with the mixing cavity (not shown in the figure). During normal operation, the cleaning pipe is in a closed state. When the concentration configuration is incorrect, the cleaning pipe is opened, the cleaning liquid is injected, and the cleaning liquid can be discharged in cooperation with the squeezing action of the bottom plate 201, which is more conducive to convenient experiments.

[0032] A solution proportioning mechanism provided in an embodiment of the present invention specifically provides a matching method between a piston cylinder 1 and a piston assembly 2. Specifically, the cavity cross section of the piston cylinder 1 can be set to be polygonal, such as Figure 1 As shown, a rectangle or the like can be used. In this case, the bottom plate 201 matches the inner cavity of the piston cylinder 1, so that the position deviation of the opening caused by the deflection of the piston assembly 2 can be avoided. Any side of the partition plate 202 can abut against the inner wall of the piston cylinder 1 to form a closed contact. In order to ensure the normal operation of the partition plate 202, a special-shaped opening matching the shape of the partition plate 202 is opened on the top of the piston cylinder 1, so that the partition plate 202 can pass through the special-shaped opening and be in sliding and sealing contact with the piston cylinder 1.

[0033] A solution proportioning mechanism provided in an embodiment of the present invention specifically provides a driving method for driving the piston assembly 2. Specifically, the second driving module 4 is set as a servo linear motion module 401. Figure 4 as well as Figure 5 As shown, a mounting block 402 is fixedly provided at the output end of the servo linear motion module 401, and the movable direction of the mounting block 402 is set parallel to the axial direction of the piston cylinder 1. Considering that the partition plate 202 is a special-shaped plate, in order to avoid the concentration of force points causing the partition plate 202 to hit the side of the piston cylinder 1, resulting in increased obstruction and affecting the service life of the mechanism, a pressure block 403 is rotatably provided on the mounting block 402. The pressure block 403 includes two pressure edges connected to each other at two end points. The connection point position of the two pressure edges is rotatably provided on the mounting block 402, and the bottom ends of the two pressure edges are respectively pressed and held On both sides of the partition plate 202, when the second drive module 4 moves in the drive mounting block 402, the bottom ends of the two pressing edges of the pressing block 403 can be used to press on both sides of the partition plate 202. When the force on one side becomes larger, the angle of the other side of the bottom angle of the pressing edge can be changed due to the rotation effect and a relatively stable force can be applied. That is, the pressing block 403 can apply a relatively uniform force to both sides of the partition plate 202 at the same time through one drive. Since the force application position is close to the side, the power for the stable sliding of the partition plate 202 can be relatively stably increased, which is helpful to prevent the piston assembly 2 from getting stuck during the operation. In order to enable the bottom plate 201 to move upward, movable plates 6 are installed on the tops of both sides of the partition plate 202, and movable openings are opened on each movable plate 6, such as Figure 4 As shown, an action rod 7 is installed at the bottom of the pressing edge, and each action rod 7 is limited to be movably set within the limited range of the movable opening, that is, when pressing or lifting, the action rod 7 can apply pressure or tension to the movable sheet 6, thereby driving the bottom plate 201 to perform corresponding lifting actions. If it is considered that the position accuracy of the bottom plate 201 is difficult to guarantee using this method, the moving position of the piston assembly 2 can be directly detected by the corresponding position sensor, and the specific configuration method is not limited here.

[0034] A solution proportioning mechanism provided in an embodiment of the present invention, in order to facilitate the installation of the piston assembly 2 and the observation of the mixing process of the mixed liquid, a piston cylinder 1 can be provided here, including an upper connecting part 101 and a lower connecting part 102 that can be assembled with each other, which can be locked by means of bolts or other structural means, and guide grooves whose positions match each other are provided on the upper connecting part 101 and the lower connecting part 102, and guide ribs that can match the shape of the guide grooves are provided on the sides of the partition plate 202, so that when the piston assembly 2 is installed, the partition plate 202 can be assembled and plugged along the position of the guide groove to ensure the accuracy of the installation position, and the sealing can be achieved by arranging a sealing gasket on the side of the guide groove to seal and cooperate with the guide rib, and other sealing cooperation methods are not limited here. In order to facilitate the observation of the mixing state, an observation window 103 can be provided on the upper connecting part 101 and the lower connecting part 102, and a display scale is provided on the side of the observation window 103, so that the experimental operator can directly observe the volume and liquid changes. When a servo motor is used to precisely control the position of the bottom plate 201, the volume of the blocking rod 5 in the lower mixing chamber should also be considered. For example, if the inner cavity of the piston cylinder 1 is a 10mm×10mm square, if the volume of the lower mixing chamber needs to be increased by 1000ml, the bottom plate needs to be moved up by 10mm. However, considering the actual surface area of ​​the blocking rod 5, if the surface area of ​​the cross section of the blocking rod 5 is 10mm 2 , then the actual displacement distance of the actual bottom plate 201 should be 1000 / (100-10), which is about 11.111mm. At this time, the actual marked scale line position height is compounded with the corresponding liquid volume change (that is, although the bottom plate 201 rises 11.111mm, the marked scale reading only rises 10mm, so that personnel can observe and measure intuitively).

[0035] The specific operation method of the above mechanism for mixing and proportioning the solution is as follows:

[0036] Step 1: Raise the bottom plate 201 to the required volume position;

[0037] Step 2: Pull out the corresponding blocking rod 5 from the opening position of the bottom plate to inject the corresponding proportioned solution, and after the mixing chamber is filled, the blocking rod 5 is reset to block the opening position;

[0038] Step 3: Repeat steps 1 and 2 until the mixing chamber below is mixed with a solution of the required concentration;

[0039] Step 4: Ensure that all blocking rods 5 block the openings of the bottom plate 201 and then drive the bottom plate 201 to move downward as a whole, so as to discharge the mixed solution in the lower mixing chamber from the liquid outlet.

[0040] (Optional) Step 5: Keep part of the stock solution in step 4, repeat steps 1 to 4, dilute the solution obtained in step 4 a second time, and prepare solutions with multiple concentration gradients in sequence from high to low. Embodiment 2

[0041] The present invention provides a solution dilution device, which is used to facilitate the direct preparation of solutions of various concentrations. It can realize the automatic preparation of solutions of different concentrations without the need for personnel configuration, thereby further simplifying the workload of personnel. In order to realize the structural function of the device, a solution proportioning mechanism including any one of the embodiments of the first embodiment is provided. In addition, the device also includes a pipette 8 and a third driving module 9 for driving the pipette 8 to move within a spatial range. Figure 1As shown, the third driving module 9 can be in the form of a manipulator or other movable mechanical arm. The specific structure of the manipulator is not limited here. In order to realize the automatic supply of solutions of different concentrations, a liquid inlet pipe 10 connected to the liquid outlet of the piston cylinder 1 and an air inlet pipe 11 for connecting to external pressure gas are provided on the pipette 8, and the liquid inlet pipe 10 and the air inlet pipe 11 are both capable of controlling the on-off of their passages (such as using corresponding solenoid valves on the corresponding passages, etc.), and a three-way solenoid valve 12 is installed and connected to the liquid outlet of the piston cylinder 1, and the other two ways of the three-way solenoid valve 12 are respectively connected to the liquid inlet pipe 10 and the liquid outlet pipe 13, and the liquid outlet pipe 13 is used to discharge the reflux liquid of the liquid inlet pipe 10. Through the arrangement of the above mechanism, when liquid needs to be supplied, it is mainly supplied stably by the pipette 8, and the power for supplying liquid comes from the gas in the air inlet pipe 11. When in use, the solution proportioning mechanism provided in Example 1 is used to prepare a solution of corresponding concentration, and the extrusion action of the bottom plate 201 supplies the mixed liquid to the pipette 8 through the liquid inlet pipe 10. After waiting for liquid to seep out from the bottom of the pipette 8, the three-way solenoid valve 12 closes the liquid supply passage, and the air inlet pipe 11 is used to accurately supply gas, thereby controlling the liquid output of the mixed liquid inside the pipette 8. After a mixed liquid of a certain concentration is used up, the mixed liquid in the pipette 8 can be completely discharged by the air inlet pipe 11, and the mixed liquid of the second concentration can be supplied to the pipette 8. As a preferred embodiment, the pipette 8 can also be cleaned by the cleaning pipeline used in Example 1, and the pipette 8 is directly cleaned by the control of the three-way solenoid valve 12, thereby facilitating the maintenance of the pipette 8. In order to avoid residual liquid in the liquid inlet pipe 10 affecting the subsequent concentration ratio, the three-way solenoid valve 12 can also directly open the passage where the liquid inlet pipe 10 and the liquid outlet pipe 13 are located, so that the unused mixed liquid in the liquid inlet pipe 10 can directly flow back and out, avoiding affecting the concentration of the next liquid supply. In some other embodiments, when the concentration of the mixed liquid is incorrectly configured, in order to facilitate the direct discharge of the mixed liquid with the wrong concentration from the mixing cavity, the three-way solenoid valve 12 can be replaced by solenoid valves independently set on three pipelines. These independent solenoid valves correspond to the control of on and off, respectively, and can achieve the connection of the corresponding passages in different usage scenarios, which can include normal liquid supply, liquid supply reflux, and connection work for mixing failure discharge.

[0042] A solution dilution device provided in an embodiment of the present invention, in order to increase the volume of the supplied original liquid, the device is also provided with a liquid supply box 14, the liquid supply box 14 is provided for separating and storing different types of solutions, and the bottom of each solution storage chamber is provided with a connecting tube 15 connected to the corresponding liquid supply cavity. Under normal circumstances, the connecting tube 15 and the liquid supply cavity are filled with sufficient liquid to be mixed, and the bubbles generated under the bottom plate 201 will also enter the liquid supply box 14 through the connecting tube 15, that is, the volume of the piston cylinder 1 can be reduced as much as possible while the capacity of the standby liquid can be increased as much as possible, which is conducive to the continuous configuration of mixed solutions of different concentrations. In order to allow the liquid to flow into the mixing cavity normally, there should be no negative pressure in the liquid supply box 14. However, the connection with the external environment may easily cause the original liquid to be contaminated. For this reason, a liquid supply port is provided at the top of the liquid supply box 14, and a box cover 16 is installed at the position of the liquid supply port. An opening is provided on the box cover 16, and finally a filter layer 17 is provided at the opening position. The filter layer 17 can prevent impurities from entering the box while balancing the internal air pressure of the box, which is beneficial to ensure that the original liquid is not contaminated.

[0043] A solution dilution device provided by an embodiment of the present invention takes into account that in the configuration of the solution, it is not convenient to directly discharge the excess solution in the pipette 8. In order to facilitate the collection of samples and the discharge of waste liquid, new liquid is injected into the pipette 8. The device is also provided with a 96-well plate 18 and a waste liquid collection box 19. At this time, the third drive module 9 can at least execute the action program of the pipette 8 according to the injection method of the 96-well plate 18, and the waste liquid collection box 19 is used to temporarily store the waste liquid from the pipette 8.

[0044] The specific steps of diluting the solution by the above device are as follows:

[0045] Step 1: The solution of required concentration is squeezed out from the piston cylinder 1 and enters the pipette 8;

[0046] Step 2: Block the liquid inlet pipe 10 where the pipette 8 is located, open the passage where the air inlet pipe 11 is located, and supply a certain amount of gas to the inside of the pipette 8 according to the demand to discharge the solution inside the pipette 8 in a certain amount. During the discharge process, the third driving module 9 controls the pipette to move within the plane range, and quickly injects multiple sample solutions into different containers;

[0047] Step 3: After the solution sample is prepared, the liquid in the pipette 8 is emptied by air supply, and the air inlet pipe 11 is blocked after the action is completed;

[0048] Step 4: Continue to prepare the liquid of the required concentration, and discharge the residual liquid in the liquid inlet tube 10 of the pipette 8 by pressing down the bottom plate 201 until the interior of the pipette 8 is filled with the mixed liquid of the required concentration;

[0049] Step 5: Repeat steps 2 to 4 until sample solutions of different concentrations are injected into multiple containers.

[0050] In the above step 4, the residual liquid in the liquid inlet pipe 10 can also be discharged by reflux, which can be done by reasonably setting the corresponding reflux pipeline. The specific implementation method will not be further elaborated here.

[0051] The above are only preferred embodiments of the present invention. It should be pointed out that, for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A solution proportioning mechanism, characterized in that: It comprises a piston cylinder (1), a piston assembly (2) arranged in the piston cylinder (1), a first driving module (3), and a second driving module (4) for driving the piston assembly (2) to move along the axis direction of the piston cylinder (1); The piston assembly (2) comprises a bottom plate (201) and a partition plate (202) arranged on the bottom plate (201); the bottom plate (201) matches the inner cavity cross section of the piston cylinder (1); the bottom plate (201) is used to divide the interior of the piston cylinder (1) into an upper cavity and a lower mixing cavity; the partition plate (202) is used to divide the upper cavity into a plurality of mutually unconnected liquid supply cavities; the partition plate (202) can slide through the upper half of the piston cylinder (1) and maintain the sealing of each liquid supply cavity; and the bottom plate (201) is provided with an opening for connecting each liquid supply cavity to the lower mixing cavity; The bottom of the piston cylinder (1) is provided with sealing holes corresponding to the positions of the respective openings, the sealing holes having the same inner diameter as the openings, a blocking rod (5) being slidably arranged on each sealing hole, and the first driving module (3) being used to drive each blocking rod (5) to move along the axial direction of the sealing hole and to be able to block the openings provided on the bottom plate (201); A liquid supply portion is provided at the top of the piston cylinder (1), and the liquid supply portion is used to supply different types of liquids to be mixed to each liquid supply cavity, and a liquid outlet portion is provided at the bottom of the piston cylinder (1); The second driving module (4) is a servo linear motion module (401), and a mounting block (402) is fixedly arranged at the output end of the servo linear motion module (401). The movable direction of the mounting block (402) is parallel to the axial direction of the piston cylinder (1). A pressing block (403) is rotatably arranged on the mounting block (402), and the pressing block (403) includes two pressing edges whose end points are connected to each other. The connection points of the two pressing edges are rotatably arranged on the mounting block (402), and the bottom ends of the two pressing edges are respectively pressed on two sides of the partition plate (202).

2. A solution proportioning mechanism according to claim 1, characterized in that: The cavity cross-section of the piston cylinder (1) is polygonal, and any side of the partition plate (202) can abut against the inner wall of the piston cylinder (1). The top of the piston cylinder (1) is provided with a special-shaped opening that matches the shape of the partition plate (202), and the partition plate (202) can penetrate the special-shaped opening and be in sliding and sealing contact with the piston cylinder (1).

3. A solution proportioning mechanism according to claim 2, characterized in that: Movable plates (6) are installed at the top of both sides of the partition plate (202), each movable plate (6) is provided with a movable opening, and an action rod (7) is installed at the bottom end of the two pressing edges, each action rod (7) is movably arranged within a limited range of the movable opening.

4. A solution proportioning mechanism according to claim 1, characterized in that: The piston cylinder (1) comprises an upper connecting portion (101) and a lower connecting portion (102) which can be assembled with each other. The upper connecting portion (101) and the lower connecting portion (102) are both provided with guide grooves whose positions match each other. The side of the partition plate (202) is provided with guide ribs which can match the shape of the guide grooves. The upper connecting portion (101) and the lower connecting portion (102) are both provided with observation windows (103). The side of the observation window (103) is provided with a display scale.

5. A solution dilution device, comprising the solution proportioning mechanism according to any one of claims 1 to 4, characterized in that: The invention also comprises a pipette (8) and a third driving module (9) for driving the pipette (8) to move within a spatial range. The pipette (8) is provided with a liquid inlet pipe (10) connected to the liquid outlet of the piston cylinder (1) and an air inlet pipe (11) for connecting to external pressure gas. The liquid inlet pipe (10) and the air inlet pipe (11) are both capable of controlling the opening and closing of their passages. A three-way solenoid valve (12) is installed at the liquid outlet of the piston cylinder (1). The other two ports of the three-way solenoid valve (12) are respectively connected to the liquid inlet pipe (10) and the liquid outlet pipe (13). The liquid outlet pipe (13) is used to discharge the refluxed liquid of the liquid inlet pipe (10).

6. A solution dilution device according to claim 5, characterized in that: It also includes a liquid supply box (14), which is used to store different types of solutions separately, and each solution storage chamber is provided with a connecting pipe (15) at the bottom thereof, which is connected to the corresponding liquid supply cavity.

7. A solution dilution device according to claim 6, characterized in that: A liquid supply port is provided at the top of the liquid supply box (14), a box cover (16) is installed at the position of the liquid supply port, an opening is provided on the box cover (16), and a filter layer (17) is provided at the position of the opening.

8. A solution dilution device according to claim 7, characterized in that: It also includes a 96-well plate (18) and a waste liquid collection box (19). The third drive module (9) is at least capable of executing an action program for the pipette (8) to perform actions according to the liquid injection method of the 96-well plate (18). The waste liquid collection box (19) is used to temporarily store waste liquid from the pipette (8).

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