A kind of tocopherol glucoside and its preparation process

Abstract

The present invention belongs to the field of bio-fermentation synthetic drugs, and specifically relates to a method for preparing tocopherol glucoside. The synthesis method of the present invention comprises the following steps: (1) preparing an enzyme catalyst; (2) performing an enzymatic conversion reaction; (3) performing ion resin purification; (4) removing impurities; (5) performing a demagnetization treatment; and (6) performing crystallization purification. The synthesis process provided by the present invention is simple and highly operable. The prepared tocopherol glucoside is highly pure, with a total product yield of >30% and a purity of >95.0%.

Description

Technical Field

[0001] The invention belongs to the field of bio-fermentation synthetic medicines, and particularly relates to tocopherol glucoside and a preparation process thereof. Background Art

[0002] Tocopherol is one of the vitamins necessary for normal life activities in the human body. It can promote the activation of human physiological functions, enhance the secretion of progesterone, promote the effectiveness of oxygen consumption in the human body, inhibit fat deposition and the production of lipid peroxides in the body, and activate human cell metabolism. It is an important type of physiologically active substance, but it is extremely easy to be oxidized and inconvenient to store. Tocopherol glucoside products can make up for the deficiency of tocopherol and expand the application field of tocopherol.

[0003] Tocopheryl glucoside not only improves water solubility, but also exhibits a variety of biological activities, including antiviral activity, the ability to combat human immunodeficiency, and anti-aging effects. In recent years, the application of tocopheryl glucoside in the medical field has become increasingly widespread, specifically in the following aspects: (1) It is suitable for the treatment of arthritis and vitamin E deficiency; (2) It can improve symptoms such as muscle dystrophy, skin inflammation and muscle weakness, and improve myocardial oxygen utilization; (3) Because of its antiviral and anti-human immunodeficiency properties, it is used in related treatments; (4) As a highly effective antioxidant, tocopheryl glucoside is also used to prevent and treat HIV disease; (5) It not only retains the activity of tocopherol, but is also non-toxic to the human body under normal conditions, suitable for intravenous injection and parenteral injection, and its activity is higher than that of oral tocopherol; (6) In addition, tocopheryl glycoside compounds also show certain anti-cancer and anti-aging effects.

[0004] In the cosmetics field, tocopheryl glucoside is also widely used: (1) in hair care products, it is widely used as a hair growth and nourishing agent; (2) in oral hygiene products, it acts as an oral cleanser; (3) because tocopheryl glucoside can absorb ultraviolet rays and protect the skin, it is also used in skin care products such as sunscreen.

[0005] In early studies, tocopheryl glucoside was synthesized by condensation reaction of tocopherol with glucose using toluenesulfonic acid or metal halides as catalysts. However, these methods suffered from low yield, poor selectivity, high raw material consumption, difficulty in product isolation, long protection and deprotection reaction times, and the tendency to produce side reactions.

[0006] In recent years, researchers have begun to try to use different proton acids as catalysts to prepare tocopherol glucoside. Compared with earlier synthesis methods, the new method reduces the production of by-products, but the yield still needs to be improved. Summary of the Invention

[0007] In order to solve the above technical problems, the present invention provides a process for synthesizing tocopheryl glucoside using biological enzyme catalysis. Nanocrystalline soft magnetic materials are used to prepare enzyme catalysts, and enzyme conversion reactions are carried out. After purification and impurity removal, the product tocopheryl glucoside is obtained.

[0008] The technical solutions of the present invention are as follows:

[0009] A tocopherol glucoside is mainly prepared by the following steps:

[0010] (1) Preparation of enzyme catalyst: adding biological enzyme to a buffer solution with a pH of 7.0, then adding nanocrystalline soft magnetic material, stirring at 25-35°C for 2-4 hours, filtering out the buffer solution to obtain the enzyme catalyst, and rinsing the enzyme catalyst with purified water;

[0011] (2) Enzymatic conversion reaction: starch, glucose, maltose, sucrose, or dextrin is used as a glycosyl donor and dissolved in purified water, tocopherol is added as a glycosyl acceptor, the mixture is stirred evenly, and the pH is adjusted to 5.5 to 6.5. The enzyme catalyst prepared in (1) is then added, and the mixture is reacted at 20 to 50° C. for 12 to 48 hours to obtain a reaction solution;

[0012] (3) Ion resin purification: After the reaction is completed, the enzyme catalyst is filtered out, and then saccharifying enzyme is added to the reaction solution and hydrolyzed at 45-55°C for 5-7 hours. After the hydrolysis is completed, the reaction solution is diluted 1-fold and purified by removing unreacted tocopherol through ion exchange resin;

[0013] (4) impurity removal: the reaction solution purified in (3) is filtered through a nanofiltration membrane to remove excess sugar raw materials, and then the pigment is removed through activated carbon;

[0014] (5) Demagnetization treatment: The filtrate after impurity removal in (4) is treated with a high-efficiency magnetic filter to eliminate the influence of the nanocrystalline soft magnetic material on the reaction solution;

[0015] (6) Crystallization purification: The filtrate after demagnetization treatment in (5) is concentrated under reduced pressure to 30% to 50% of the original volume, and the crude product is obtained by cooling and crystallizing once. An alcohol solvent is then added to dissolve the crude product, and the crude product is obtained by cooling and crystallizing twice to obtain a tocopherol glucoside content of more than 95%.

[0016] Preferably, the biological enzyme in (1) is selected from any one of 3-hydroxy-3-methylglutaryl coenzyme, dammarene diol-II synthase, L-arabinose isomerase, carbon glycosyltransferase, α-amylase, α-glucosyltransferase, yeast lipase, isomaltose glucosyltransferase, and sucrase.

[0017] Preferably, the nanocrystalline soft magnetic material in (1) is any material that can adsorb biological enzymes on the surface without inactivating the biological enzymes, and is selected from at least one of grain-oriented silicon steel (GO), grain-non-oriented silicon steel (NGO), ferrite (Mn-Zn and Ni-Zn), iron alloy powder (Fe-Si-Al, Fe-Si and Fe-Ni-MO, etc.), nanocrystalline ribbon (Fe-Si-B-Cu-Nb) and amorphous ribbon (Fe-Si-B and Co-Fe, etc.).

[0018] Preferably, in (1), the buffer solution is a disodium hydrogen phosphate solution; the volume mass ratio of the buffer solution to the biological enzyme is 4 mL: 1-1.5 g; and the mass ratio of the biological enzyme to the nanocrystalline soft magnetic material is 1: 1-2.

[0019] Preferably, in the preparation process of the enzyme catalyst in (1), the biological enzyme is diluted with a buffer solution, the carrier is added, and stirred at 30°C for 3 to 4 hours. The liquid is then filtered off, and the fixed carrier is washed with pure water. The prepared enzyme catalyst can be reused multiple times.

[0020] Preferably, in (2), the mass ratio of the glycosyl donor to the enzyme catalyst is 1:0.01-1; the mass ratio of the glycosyl acceptor to the glycosyl donor is 1:2-5.

[0021] Preferably, in (3), the mass ratio of saccharifying enzyme to tocopherol in (2) is 3:20-50; and the ion exchange resin is a weakly basic ion exchange resin.

[0022] Preferably, the nanofiltration membrane in (4) is a spiral membrane with a molecular weight cut-off of 200 Da.

[0023] Preferably, the magnetic filter in (5) is an SXC magnetic filter.

[0024] Preferably, during the crystallization purification in (6), the temperature of both recrystallizations is lowered to 5-10°C.

[0025] Since the present invention relates to bioenzyme catalysis, the nanocrystalline soft magnetic material in the present invention (1) is selected to be a material that can adsorb the bioenzyme on its surface without inactivating the bioenzyme. In the process of preparing the enzyme catalyst, the bioenzyme is diluted with a buffer solution to 20% of its original mass concentration, and then a carrier is added. The solution is stirred at 30°C for 3 to 4 hours. During this process, the temperature must be strictly controlled to prevent the bioenzyme from inactivating.

[0026] The beneficial effects of the present invention are:

[0027] (1) The synthetic process of the present invention is simple, the content of by-products in each step is low, the total yield of the product is greater than 30%, and the purity is greater than 95.0%, which is more suitable for industrial production;

[0028] (2) The entire synthesis process of the present invention does not involve high pressure and high temperature, and has high safety and operability. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is the liquid chromatogram of the target product after purification in Example 1;

[0030] Figure 2 This is the liquid chromatogram of the target product after purification in Example 2;

[0031] Figure 3 This is the liquid chromatogram of the target product after purification in Comparative Example 1. DETAILED DESCRIPTION

[0032] In order to enable those skilled in the art to better understand the present invention, the present invention will be further explained in conjunction with specific embodiments.

[0033] Example 1

[0034] A process for preparing tocopherol glucoside comprises the following steps:

[0035] (1) Preparation of enzyme catalyst: 100 g of 3-hydroxy-3-methylglutaryl coenzyme was added to 400 mL of sodium hydrogen phosphate buffer at pH 7.0, followed by 100 g of ferrite (Mn-Zn) particles. The mixture was stirred at 30°C for 3 h to immobilize the 3-hydroxy-3-methylglutaryl coenzyme on the ferrite (Mn-Zn) particles to obtain an enzyme catalyst. The buffer was then filtered off and the enzyme catalyst was rinsed with purified water.

[0036] (2) Enzymatic conversion reaction: 1 kg of starch was dissolved in 5 L of purified water, 200 g of tocopherol was added, starch was used as a glycosyl donor, and tocopherol was used as a glycosyl acceptor, and the mixture was stirred evenly. The pH of the reaction solution was adjusted to 5.5. After stirring evenly, the enzyme catalyst prepared in (1) was added and the mixture was reacted at 50°C for 48 h.

[0037] (3) Ion resin purification: After the reaction is completed, the enzyme catalyst solid is filtered off, and 30 g of saccharifying enzyme is added to the reaction solution and hydrolyzed at 50°C for 6 h. After the hydrolysis is completed, the reaction solution is diluted 1-fold with disodium hydrogen phosphate solution and purified by a resin column filled with weakly basic ion exchange resin to remove unreacted tocopherol;

[0038] (4) impurity removal: the reaction solution purified in (3) is filtered through a spiral nanofiltration membrane to remove excess sugar raw materials, and then impurities such as pigments are removed by activated carbon adsorption;

[0039] (5) Demagnetization treatment: The filtrate after impurity removal in (4) is treated with a high-efficiency magnetic filter to eliminate the influence of the nanocrystalline soft magnetic material on the reaction solution;

[0040] (6) Crystallization purification: The filtrate after demagnetization treatment in (5) was concentrated to 50% of the original volume by reduced pressure evaporation (-0.1 MPa, 50°C), and the temperature was cooled to 10°C once to obtain a crude tocopherol glucoside product. The crude product was then dissolved in ethanol and cooled to 10°C for a second time to crystallize to obtain a tocopherol glucoside sample with a purity of 95% and a yield of 35%.

[0041] The tocopherol glucoside sample obtained in this example was analyzed by liquid chromatography. The analysis results are shown in Table 1 and Figure 1 shown.

[0042] Table 1 Liquid chromatography information of tocopherol glucoside obtained in Example 1

[0043]

[0044] Example 2

[0045] A process for preparing tocopherol glucoside comprises the following steps:

[0046] (1) Preparation of enzyme catalyst: 100 g of dammarenediol-II synthase was added to 400 mL of pH 7.0 sodium hydrogen phosphate buffer, followed by 200 g of nanocrystalline ribbon (Fe-Si-B-Cu-Nb) particles. The mixture was stirred at 30°C for 3 h, and the buffer was filtered. The nanocrystalline soft magnetic material was rinsed with purified water.

[0047] (2) Enzymatic conversion reaction: 1 kg maltose was dissolved in 5 L purified water, 500 g tocopherol was added, stirred evenly and the pH of the reaction solution was adjusted to 5.5. The treated enzyme catalyst was added to the reaction solution and reacted at 50 °C for 20 h.

[0048] (3) Ion resin purification: After the reaction is completed, the enzyme catalyst solid is filtered out, and 30 g of saccharifying enzyme is added to the reaction solution and hydrolyzed at 50 ° C for 6 h. After the hydrolysis is completed, the reaction solution is diluted 1 times and purified by a resin column filled with weakly basic ion exchange resin;

[0049] (4) Impurity removal: The filtrate passes through the spiral nanofiltration membrane and is adsorbed by activated carbon to remove impurities such as pigments;

[0050] (5) Demagnetization treatment: Finally, the final liquid is demagnetized by a magnetic filter;

[0051] (6) Crystallization purification: The feed solution was concentrated to 45% by reduced pressure evaporation (-0.1 MPa, 50°C), and the temperature was lowered to 10°C to obtain a crude tocopherol glucoside product. The crude product was then dissolved in ethanol, cooled to 10°C, and crystallized again to obtain a tocopherol glucoside sample with a purity of 96% and a yield of 32%.

[0052] The tocopherol glucoside sample obtained in this embodiment was analyzed by liquid chromatography. The analysis results are shown in Table 2 and Table 3. Figure 2 shown.

[0053] Table 2 Liquid chromatography information of tocopherol glucoside obtained in Example 2

[0054]

[0055] Example 3

[0056] A process for preparing tocopherol glucoside comprises the following steps:

[0057] (1) Preparation of enzyme catalyst: 100 g of L-arabinose isomerase was added to 400 mL of pH 7.0 sodium hydrogen phosphate buffer, followed by 150 g of grain-oriented silicon steel (GO) particles. The mixture was stirred at 30 °C for 4 h, and then the buffer was filtered and the nanocrystalline soft magnetic material was rinsed with purified water.

[0058] (2) Enzymatic conversion reaction: 1 kg of sucrose was dissolved in 5 L of purified water, 200 g of tocopherol was added, and the mixture was stirred evenly. The pH of the reaction solution was adjusted to 5.5, and the treated enzyme catalyst was added to the reaction solution. The reaction was carried out at 50 °C for 36 h.

[0059] (3) Ion resin purification: After the reaction is completed, the enzyme catalyst solid is filtered off, 30 g of saccharifying enzyme is added to the reaction solution and hydrolyzed at 50 °C for 6 h. The reaction solution is then diluted 1-fold and passed through a resin column filled with weakly basic ion exchange resin;

[0060] (4) Impurity removal: The filtrate passes through the spiral nanofiltration membrane and is adsorbed by activated carbon to remove impurities such as pigments;

[0061] (5) Demagnetization treatment: Finally, the final liquid is demagnetized by a magnetic filter;

[0062] (6) Crystallization purification: The feed solution was concentrated to 30% by reduced pressure evaporation (-0.1 MPa, 50°C), and the temperature was lowered to 10°C to obtain a crude tocopherol glucoside product. The crude product was then dissolved in ethanol, cooled to 10°C, and crystallized again to obtain a tocopherol glucoside sample with a purity of 95% and a yield of 34%.

[0063] Example 4

[0064] A process for preparing tocopherol glucoside comprises the following steps:

[0065] (1) Preparation of enzyme catalyst: 150 g of glycosyltransferase was added to 400 mL of pH 7.0 sodium hydrogen phosphate buffer, followed by 200 g of amorphous ribbon (Fe-Si-B) particles. The mixture was stirred at 30°C for 4.5 h, and the buffer was filtered. The nanocrystalline soft magnetic material was rinsed with purified water.

[0066] (2) Enzymatic conversion reaction: 1 kg of sucrose was dissolved in 5 L of purified water, 300 g of tocopherol was added, the pH of the reaction solution was adjusted to 5.5, the treated enzyme catalyst was added to the reaction solution, and the reaction was carried out at 50 °C for 40 h;

[0067] (3) Ion resin purification: After the reaction is completed, the enzyme catalyst solid is filtered off, 30 g of saccharifying enzyme is added to the reaction solution and hydrolyzed at 50 °C for 6 h. The reaction solution is then diluted 1-fold and passed through a resin column filled with weakly basic ion exchange resin;

[0068] (4) Impurity removal: The filtrate passes through the spiral nanofiltration membrane and is adsorbed by activated carbon to remove impurities such as pigments;

[0069] (5) Demagnetization treatment: Finally, the final liquid is demagnetized by a magnetic filter;

[0070] (6) Crystallization purification: The feed solution was concentrated to 30% by reduced pressure evaporation (-0.1 MPa, 50°C), and the temperature was lowered to 5°C to obtain a crude tocopherol glucoside product. The crude product was then dissolved in ethanol, cooled to 5°C, and crystallized again to obtain a tocopherol glucoside sample with a purity of 95% and a yield of 33%.

[0071] Example 5 Exploration of experimental conditions

[0072] 5.1 Reaction Time

[0073] Other experimental conditions were the same as in Example 1, except that only the reaction time in the preparation step was changed. Multiple experiments were conducted, and the experimental results are shown in Table 3 below:

[0074] Table 3 Experimental results at different reaction times

[0075]

[0076]

[0077] When the reaction time is less than 12 h, the reaction conversion rate is not high and the overall yield is low. When the reaction time is greater than 48 h, the reaction effect is almost the same as that of the reaction at about 48 h, with no significant change.

[0078] According to the test results, when the reaction time is controlled within 12h~48h, the reaction conversion rate is relatively high, and the reaction yield and product purity are good.

[0079] 5.2pH

[0080] Other experimental conditions were the same as those in Example 1. Only the single variable of the reaction pH in the preparation step (2) was changed. Multiple experiments were conducted. The experimental results are shown in Table 4 below:

[0081] Table 4 Experimental results at different pH

[0082] Reaction pH Reaction conversion rate Product purity Product yield 4 59.7％ 68.1％ 16.4％ 5 72.3％ 70.6％ 23.2％ 5.5 84.5％ 94.9％ 31.1％ 6 86.7％ 95.1％ 32.5％ 6.5 84.3％ 95.8％ 35.2％ 7 81.7％ 92.3％ 27.3％ 8 71.6％ 75.9％ 23.8％ 9 67.7％ 72.4％ 24.1％

[0083] In step (2), when the reaction pH is less than 5.5 and the reaction pH is greater than 6.5, the activity of the biological enzyme will decrease, affecting the overall reaction.

[0084] According to the reaction results, when the reaction pH is controlled between 5.5 and 6.5, the reaction conversion rate is relatively high, and the reaction yield and product purity are good.

[0085] 5.3 Temperature

[0086] Other experimental conditions were the same as those in Example 1, with only the reaction temperature in the preparation step (2) being changed. Multiple experiments were conducted, and the experimental results are shown in Table 5:

[0087] Table 5 Experimental results at different temperatures

[0088] Reaction temperature Reaction conversion rate Product purity Product yield 15 58.9％ 67.1％ 17.4％ 18 70.3％ 71.6％ 23.9％ 20 84.6％ 95.4％ 31.2％ 25 86.7％ 95.3％ 33.5％ 45 87.1％ 95.6％ 35.7％ 50 84.8％ 95.1％ 32.3％ 52 73.6％ 75.2％ 24.1％ 55 69.7％ 73.5％ 23.5％

[0089] In step (2), when the reaction temperature is greater than 50°C, the enzyme will be partially inactivated, thereby reducing the reaction efficiency. When the temperature is less than 20°C, the enzyme activity is low, the reaction time will increase, and the overall reaction efficiency will be reduced.

[0090] In summary, the reaction temperature in step (2) is preferably 20-50°C, the reaction time is 12-48h, and the pH is 5.5-6.5. At this time, the total yield and purity of the obtained product are the highest, and the impurities are the least.

[0091] 5.4 Dosage

[0092] Other experimental conditions were the same as those in Example 1. Only the single variable of the mass ratio of the biological enzyme and the carrier in the preparation step (1) was changed. Multiple experiments were conducted. The experimental results are shown in Table 6 below:

[0093] Table 6 Experimental results under different mass ratios of biological enzymes and carriers

[0094]

[0095]

[0096] In step (1), when the mass ratio of the biological enzyme to the carrier is in the range of 1:1 to 1:2, the biological enzyme adsorbed on the carrier is relatively uniform, and the prepared enzyme catalyst has good stability.

[0097] Other experimental conditions were the same as those in Example 1. The single variable, the amount of the enzyme catalyst in the preparation step (2), was changed. Multiple tests were conducted. The experimental results are shown in Table 7 below:

[0098] Table 7 Experimental results under different enzyme catalyst dosages

[0099]

[0100] In step (2), the amount of the enzyme catalyst is 0.01 to 1 times the mass of the glycosyl donor. When the amount of the enzyme catalyst is less than 0.01 times, the reaction effect is poor; when the amount of the enzyme catalyst is greater than 1 times, the reaction result is almost the same as the result when the amount is 1 times, and the change is not obvious;

[0101] Other experimental conditions were the same as those in Example 1. The single variable, the mass ratio of tocopherol to glycosyl donor in the preparation step (2), was changed. Multiple experiments were conducted. The experimental results are shown in Table 8 below:

[0102] Table 8 Experimental results at different tocopherol to glycosyl donor mass ratios

[0103]

[0104]

[0105] When the mass ratio of tocopherol to glycosyl donor in step (2) is less than 1:2, the reaction yield is not high. When the mass ratio of tocopherol to glycosyl donor is greater than 1:5, the reaction result is basically the same as the result when the mass ratio is 1:5, with no significant change.

[0106] In summary, the optimal reaction conditions are a mass ratio of biological enzyme to carrier of 1:1-2, a mass ratio of enzyme catalyst to glycosyl donor of 0.01-1 times, and a mass ratio of tocopherol to glycosyl donor of 1:2-5.

[0107] Comparative Example 1

[0108] A preparation process of tocopherol glucoside comprises the following steps: adding 100 g of alpha-amylase to 400 mL of pH 7.0 phosphate buffer, then adding 100 g of zeolite particles, stirring at 30° C. for 3 hours, then filtering the buffer, and rinsing the zeolite particles with purified water. 1 kg of starch was dissolved in 5 L of purified water, 200 g of tocopherol was added, and the reaction solution was adjusted to 5.5. The treated immobilized enzyme was added to the reaction solution and reacted at 50° C. for 48 hours. After the reaction, the zeolite particles were filtered out, and 30 g of saccharifying enzyme was added to the reaction solution and hydrolyzed at 50° C. for 6 hours. The reaction solution was then diluted 1 time and passed through a resin column equipped with a weakly alkaline ion exchange resin. The filtrate was passed through a rolled nanofiltration membrane and finally depigmented by activated carbon adsorption. The feed liquid was concentrated to 50% by reduced pressure evaporation (-0.1 MPa, 50° C.), cooled to 10° C. to obtain a crude tocopherol glucoside product. The crude product was then dissolved in ethanol, cooled and crystallized again to obtain a tocopherol glucoside sample with a purity of 92% and a yield of 27%.

[0109] The tocopherol glucoside sample obtained in this embodiment was analyzed by liquid chromatography. The analysis results are shown in Table 9 and Figure 3 shown.

[0110] Table 9 Liquid chromatography information of tocopherol glucoside obtained in the comparative example

[0111]

Claims

1. A method for preparing tocopherol glucoside, characterized in that: The following steps are involved: (1) Preparation of enzyme catalyst: add biological enzyme to a buffer solution with a pH of 7.0, then add nanocrystalline soft magnetic material, stir at 25-35 °C for 2-4 h, filter out the buffer solution to obtain the enzyme catalyst, and rinse the enzyme catalyst with pure water; Wherein, the biological enzyme is selected from any one of 3-hydroxy-3-methylglutaryl coenzyme, dammarenediol-II synthase, L-arabinose isomerase, and carbon glycosyltransferase; The nanocrystalline soft magnetic material is selected from at least one of grain-oriented silicon steel, grain-non-oriented silicon steel, ferrite, ferroalloy powder, nanocrystalline ribbon and amorphous ribbon; (2) Enzymatic conversion reaction: starch, glucose, maltose, sucrose, or dextrin is used as a glycosyl donor and dissolved in purified water. Tocopherol is then added as a glycosyl acceptor and stirred evenly. The pH is adjusted to 5.5-6.

5. The enzyme catalyst prepared in (1) is then added and the reaction is carried out at 20-50°C for 12-48 hours to obtain a reaction solution. Among them, the mass ratio of glycosyl donor to enzyme catalyst is 1:0.01~1; The mass ratio of glycosyl donor to glycosyl acceptor is 2~5:1; (3) Ion resin purification: After the reaction is completed, the enzyme catalyst is filtered out, and then glucoamylase is added to the reaction solution for hydrolysis at 45-55°C for 5-7 hours. After the hydrolysis is completed, the reaction solution is diluted 1-fold and the unreacted tocopherol is removed by ion exchange resin for purification; (4) Impurity removal: The reaction solution obtained by purification in (3) is filtered through a nanofiltration membrane to remove excess sugar raw materials, and then activated carbon is used to remove pigments; (5) Demagnetization treatment: The filtrate after impurity removal in (4) is treated with a high-efficiency magnetic filter to eliminate the influence of the nanocrystalline soft magnetic material on the reaction solution; (6) Crystallization purification: The filtrate after demagnetization treatment in (5) is concentrated under reduced pressure to 30% to 50% of the original volume, and the crude product is obtained by cooling and crystallizing once. An alcohol solvent is then added to dissolve the crude product, and the crude product is obtained by cooling and crystallizing twice to obtain a tocopherol glucoside content of more than 95%.

2. The method for preparing tocopherol glucoside as claimed in claim 1, wherein: (1), the buffer solution is disodium hydrogen phosphate solution; The volume mass ratio of buffer solution to enzyme is 4mL:1~1.5g; The mass ratio of the biological enzyme to the nanocrystalline soft magnetic material is 1:1~2.

3. The method for preparing tocopherol glucoside as claimed in claim 1, wherein: (3), the mass ratio of the saccharifying enzyme to the tocopherol in (2) is 3:20-50; The ion exchange resin is a weakly basic ion exchange resin.

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