Pesticide insecticide composition and application thereof

Abstract

The invention relates to the technical field of insecticides, and discloses a pesticide insecticide composition, which is prepared from flufenamid and spinosad, the mass ratio of the metafluzamide to the spinosad is (1 to 30): (1 to 30); the insecticide composition is used for preventing and treating insect pests of crops. According to the invention, the flufenamid and the spinosad are compounded and can generate a synergistic effect, so that the formed pesticide has a good insecticidal effect and a relatively large control spectrum, and particularly has a good control effect on plutella xylostella and eggplant thrips.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticides, and in particular to a pesticide composition and application thereof. Background Art

[0002] The diamondback moth, scientific name: Plutella xylostella (Li nnaeus), belongs to the family of Lepidoptera. It harms cruciferous crops such as rapeseed, cabbage, Chinese cabbage, and radish, and occasionally potatoes, onions, ginger, tomatoes, etc. It is an important pest of vegetables worldwide and a major pest of rapeseed. In general years, the damage caused by the diamondback moth is relatively minor, but in some years, it can break out and cause disasters. In severe cases, the damage can reach the point of total crop failure. It is widely distributed in various parts of China.

[0003] The main species of eggplant thrips is palm thrips (Thrips palmi Karny). The adults and nymphs of thrips use rasp-sucking mouthparts to suck the sap from the tender tissues and organs of eggplant plants, damaging the stems, leaves, flowers and pods, causing wrinkled and deformed leaves, wilting plants, falling flowers and pods, and deformed young pods, which seriously affect the yield and quality of eggplant. It is one of the most serious pests in eggplant production.

[0004] Dimethoprim is a pyrazole amide compound, its English common name: dimethoprim; trade name: AxalionTM; IUPAC name: 1-[(1RS)-1,2-dimethylpropyl]-N-ethyl-5-methyl-N-pyridazin-4-yl-1H-pyrazole-4-carboxamide; CAS registration number: 1403615-77-9; molecular formula: C16H23N5O; Dimethoprim has excellent product performance and good systemic conductivity. It is mainly used for fruit trees and vegetables, soybeans, other legumes, cotton, cereals, potatoes and other field crops, as well as flowers and ornamental plants, to control pests such as Lepidoptera (Spodoptera exigua, Plutella xylostella, etc.), Coleoptera (beetles, corn root leaf beetles, potato leaf beetles, yellow cabbage flea beetles, etc.), Diptera (flies, mosquitoes, vegetable leafminers, etc.), Hemiptera (aphids, planthoppers, psyllids, whiteflies, etc.), Thysanoptera (thrips, orchid thrips, palm thrips, tobacco thrips, etc.), Isoptera (termites, etc.), cockroaches, ants, etc., especially effective against piercing-sucking mouthparts pests such as aphids, whiteflies, and psyllids.

[0005] Spinosad, also known as spinosad, is a macrolide biogenic compound and a secondary metabolite obtained by aerobic fermentation of soil actinomycetes. It has good insecticidal activity against a variety of lepidopteran pests, and has low toxicity to natural enemies insects and mammals. It degrades quickly in soil and water and is safe for the environment.

[0006] There are no reports on the combined use of spinosad and methoprene in the existing technology. Due to the wide planting areas of vegetables such as cabbage and eggplant, and the rapid reproduction rate of diamondback moth and eggplant thrips, their prevention and control is difficult and the damage is serious. Many of the commonly used control agents are long-lasting, resistance develops quickly, and the control effect is poor; in public reports, there is no in-depth research on the activity of spinosad and methoprene against diamondback moth and eggplant thrips. Therefore, the existing technology combines different agents and then controls pests, but there is a great deal of uncertainty in the effect of the combination of different agents. After mixing and combining, synergistic effects, additive effects, or antagonistic effects may occur; further research and development is needed. Summary of the invention

[0007] The purpose of the present invention is to solve the problems existing in the prior art and to propose a pesticide insecticide composition and its application.

[0008] In order to achieve the above object, the present invention adopts the following technical solutions:

[0009] A pesticide insecticide composition comprising methoxadone and spinosad;

[0010] The mass ratio of methipridax to spinosad is (1-30):(1-30);

[0011] The insecticide composition is used for preventing and controlling diamondback moth pests and eggplant thrips pests.

[0012] Preferably, the mass of the imipenem accounts for 6% of the mass of the pesticide; the mass of the spinosad accounts for 6% of the mass of the pesticide.

[0013] Preferably, the pesticide composition is in the form of a liquid preparation.

[0014] Preferably, it also includes auxiliary ingredients;

[0015] The auxiliary material components include the following by mass percentage:

[0016] Surfactant, 10-20%;

[0017] Antifreeze, 1-10%;

[0018] Solvent, 1-20%;

[0019] Carrier, 1~50%.

[0020] Preferably, the surfactant includes nonylphenol polyoxyethylene ether, nonylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether polyoxypropylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxypropylene polyoxyethylene ether, phenethylphenol polyoxyethylene ether phosphate, benzylphenol polyoxyethylene ether, phenethylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, lauryl alcohol polyoxyethylene ether, fatty amine polyoxyethylene ether, castor oil ethylene oxide adduct, anhydrous sorbitan fatty acid ester, anhydrous sorbitan One or more of fatty acid ester ethylene oxide adduct, calcium dodecylbenzene sulfonate, sulfated castor oil, fatty alcohol sulfate, fatty alcohol polyoxyethylene ether sulfate, fatty acid polyoxyethylene ester phosphate, alkylamine polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether formaldehyde condensate, polyvinyl alcohol monooleate, polyethylene glycol monooleate, polyoxyethylene polyoxypropylene block copolymer, polycarboxylate, polyacrylic acid, sodium alkylnaphthalene sulfonate fatty alcohol polyoxyethylene ether, sorbitan monooleate, polyoxyethylene sorbitan monooleate, and castor oil polyoxyethylene ether.

[0021] Preferably, the antifreeze agent includes one or more of ethylene glycol, glycerol, propylene glycol, urea, methanol, ethanol, isopropanol, diethylene glycol, ethylene glycol butyl ether, propylene glycol butyl ether, ethylene glycol butyl ether acetate, and isooctyl alcohol.

[0022] Preferably, the solvent includes one or more of solvent oil, toluene, xylene, methanol, ethanol, methyl oleate, cyclohexanone, vegetable oil, methyl vegetable oil, dimethylformamide, acetonitrile, polyethylene glycol, decanoic acid amide, tributyl phosphate, butyrolactone, N-long-chain alkyl pyrrolidone, octyl pyrrolidone, and isooctyl alcohol.

[0023] Preferably, the carrier is ionized water.

[0024] The invention discloses an application of a pesticide insecticide composition in preventing and controlling diamondback moth and eggplant thrips pests.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] The present invention selects methoxam and spinosad for compounding, and the two can produce a synergistic effect, thereby making the formed pesticide insecticide have good insecticidal effect and a large control spectrum, especially having a good control effect on diamondback moth and eggplant thrips. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be described clearly and completely below. If the specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0028] The embodiment of the present invention provides a pesticide insecticide, wherein the raw materials of the active ingredients of the pesticide insecticide are composed of methoxadone and spinosad, and a surfactant, an antifreeze agent, a solvent and a carrier may be optionally added.

[0029] The embodiment of the present invention specifically selects methoxam and spinosad for compounding, and the two can produce a synergistic effect, thereby making the formed pesticide insecticide have good insecticidal effect and a large control spectrum, especially having a good control effect on diamondback moth and eggplant thrips.

[0030] The surfactant provided in the embodiment of the present invention includes but is not limited to nonylphenol polyoxyethylene ether, nonylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether polyoxypropylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxypropylene polyoxyethylene ether, phenethylphenol polyoxyethylene ether phosphate, benzylphenol polyoxyethylene ether, phenethylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, lauryl alcohol polyoxyethylene ether, fatty amine polyoxyethylene ether, castor oil ethylene oxide adduct, anhydrous sorbitan fatty acid ester, anhydrous One or more of sorbitan fatty acid ester ethylene oxide adduct, calcium dodecylbenzene sulfonate, sulfated castor oil, fatty alcohol sulfate, fatty alcohol polyoxyethylene ether sulfate, fatty acid polyoxyethylene ester phosphate, alkylamine polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether formaldehyde condensate, polyvinyl alcohol monooleate, polyethylene glycol monooleate, polyoxyethylene polyoxypropylene block copolymer, polycarboxylate, polyacrylic acid, sodium alkylnaphthalene sulfonate fatty alcohol polyoxyethylene ether, sorbitan monooleate, polyoxyethylene sorbitan monooleate, and castor oil polyoxyethylene ether;

[0031] Antifreeze agents include, but are not limited to, one or more of ethylene glycol, glycerol, propylene glycol, urea, methanol, ethanol, isopropanol, diethylene glycol, ethylene glycol butyl ether, propylene glycol butyl ether, ethylene glycol butyl ether acetate, and isooctyl alcohol;

[0032] The solvent includes, but is not limited to, one or more of solvent oil, toluene, xylene, methanol, ethanol, methyl oleate, cyclohexanone, vegetable oil, methyl vegetable oil, dimethylformamide, acetonitrile, polyethylene glycol, decanoic acid amide, tributyl phosphate, butyrolactone, N-long chain alkyl pyrrolidone, octyl pyrrolidone, and isooctyl alcohol;

[0033] The carrier is deionized water.

[0034] It should be noted that the insecticide provided by the present invention is used to control the pests of crops including but not limited to diamondback moth, eggplant thrips, etc., but the pesticide insecticide has a better control effect on diamondback moth and eggplant thrips. In addition, the pesticide insecticide will not affect the normal growth of plants. The pesticide insecticide is a liquid preparation, preferably a microemulsion. It should be noted that the embodiments of the present invention only list microemulsions, but it is understandable that other types of liquid preparations are also within the scope of protection of the embodiments of the present invention.

[0035] The features and performance of the present invention are further described in detail below in conjunction with the embodiments.

[0036] 1. Formulation Example 1 12% spinosad·methoxazole microemulsion (1:1)

[0037] 6.0 g of spinosad, 6.0 g of imipenem, 8.0 g of phenethylphenol polyoxypropylene polyoxyethylene ether, 3.5 g of polyoxyethylene sorbitan monooleate, 1.2 g of castor oil polyoxyethylene ether, 2.5 g of ethylene glycol, and 7.5 g of polyethylene glycol were mixed into a uniform transparent oil phase, and deionized water was slowly added with stirring to form a uniform transparent liquid to obtain Preparation Example 1.

[0038] 2. Comparative Example 12% Spinosad Microemulsion (2%)

[0039] 2.0 g of spinosad, 4.0 g of phenethylphenol polyoxypropylene polyoxyethylene ether, 1.0 g of polyoxyethylene sorbitan monooleate, 1.0 g of castor oil polyoxyethylene ether, 1.5 g of ethylene glycol, and 2.5 g of caprylic acid amide were mixed into a uniform transparent oil phase, and deionized water was slowly added under stirring to form a uniform transparent liquid to obtain Comparative Example 1.

[0040] 3. Comparative Example 26% Methiocarb Microemulsion (6%)

[0041] Mix 6.0 g of methamidotriazole, 4.0 g of sorbitan monooleate, 2.0 g of polyoxyethylene sorbitan monooleate, 1.2 g of castor oil polyoxyethylene ether, 2.0 g of ethylene glycol, and 3.0 g of caprylic amide to form a uniform transparent oil phase, and slowly add deionized water while stirring to form a uniform transparent liquid to obtain Comparative Example 2.

[0042] Experimental Example 1 Biological Activity Test

[0043] This test was conducted with reference to Part 14: Leaf Dipping Method of Guidelines for Indoor Bioassay Tests of Insecticides and Pesticides NY / T 1154.14-2008. The effects of methoprene, spinosad and their mixed ratios on Plutella xylostella were determined to screen the optimal mixing ratio of the two insecticides.

[0044] A. Plutella xylostella (L i nnaeus) was collected from cabbage seedlings in Hanzhuang, Xiashu Town, Jurong, Zhenjiang City, Jiangsu Province, and then continuously raised indoors. The breeding conditions were a temperature of 25°C ± 1°C, a humidity of 60%-80%, a photoperiod of L:D = 16:8h, and no contact with any insecticides during the period.

[0045] B. The test agent is 90% methamidin and 90% spinosad technical

[0046] C. The mixing ratio of spinosad: anthracene is: 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5. The concentrations are shown in Table 1:

[0047] Table 1 The dosage ratio of each substance of the spinosad and anthracene insecticide composition

[0048]

[0049] D. Toxicity test method

[0050] According to NY / T 1154.14-2008 Insecticide Indoor Bioassay Test Guidelines Part 14: Leaf Dipping Method; dissolve the test agent with acetone and dilute it to the corresponding concentration with 0.1% Tween-80 aqueous solution, punch fresh, flat cabbage leaves into leaf discs with a hole puncher, immerse the leaf discs in the test solution for 10 seconds, take them out and dry them, and use tweezers to spread the leaves face down on a perforated, breathable flat-bottomed culture dish with agar added, put 20 2nd-instar diamondback moths in each dish, and let the treated diamondback moths grow on the plate. The test was carried out under the conditions of (25 ± 1) °C, relative humidity of 60% to 80%, and photoperiod of L:D = 16h:8h. A 0.1% Tween-80 aqueous solution (containing the same volume of acetone as that of the drug treatment) without the drug was used as a blank control. Each treatment concentration was repeated 4 times. After 48 hours of treatment, the number of dead insects in each tube was checked and recorded one by one. Diamondback moths that did not move or could not move normally were considered dead. The mortality rate and the corrected mortality rate were calculated at the same time, and the toxicity regression equation and LD were obtained. 50 .

[0051] Calculation formula:

[0052]

[0053]

[0054] When the mortality rate of the control drug is <5%, no correction is required. When the mortality rate is between 5% and 20%, correction is performed according to the calibration mortality formula. When the mortality rate is >20%, the test is repeated.

[0055] According to the survey data, the mortality rate and corrected mortality rate of each treatment were calculated, and the DPS software was used for data processing to calculate the LD of each agent. 50 , LD 90 , b value (standard error) and LD 50 The 95% confidence limit of the mixture was calculated according to the Sun Yunpei method.

[0056] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formula (1), formula (2) and formula (3):

[0057]

[0058] Where: ATI—actual toxicity index of the mixture;

[0059] S—LD of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0060] M—LD of the mixture 50 , the unit is milligrams per liter (mg / L).

[0061] TTI=A×P A +B×P B (2)

[0062] Where: TTI—theoretical toxicity index of mixture;

[0063] A—Agent toxicity index;

[0064] P A —The percentage of agent A in the mixture, in percentage (%);

[0065] B—B agent toxicity index;

[0066] P B —The percentage of agent B in the mixture, in percentage (%).

[0067]

[0068] In the formula: CTC—co-toxicity coefficient; ATI—measured toxicity index of the mixture; TTI—theoretical toxicity index of the mixture.

[0069] The co-toxicity coefficient (CTC) of the combination ≥120 indicates a synergistic effect; CTC ≤80 indicates an antagonistic effect; 80 < CTC <120 indicates an additive effect.

[0070] E. Test results Table 2:

[0071] Table 2 Toxicity test results of 9 mixtures of methoxazole and spinosad

[0072]

[0073] The study showed (Table 2) that the effective inhibitory concentration LD50 of 9 mixtures (spinosad:methoxam = 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5) against Plutella xylostella was 50 They were 1.31, 1.29, 1.24, 1.23, 1.41, 2.02, 2.45, 2.84 and 3.21 mg / L respectively. The activities were between the toxicity of two single doses. The co-toxicity coefficients (CTC) were 109.07, 114.47, 125.37, 138.58, 149.77, 137.37, 134.36, 130.49 and 126.02 respectively. It can be seen that the ratios of spinosad: methoprenaline of 3:1, 2:1, 1:1, 1:2, 1:3, 1:4 and 1:5 all showed synergistic effects; the ratios of spinosad: methoprenaline of 5:1 and 4:1 showed additive effects, among which the synergistic effect of spinosad: methoprenaline ratio of 1:1 was the best.

[0074] Experimental Example 2 Biological Activity Test

[0075] This experiment was carried out with reference to the leaf tube film method of NY / T 3680-2020 Technical Specification for Monitoring Resistance of Western Flower Thrips. The effects of methipridax, spinosad and their mixed products in different mass ratios on eggplant thrips (Thripspalmi) were determined to screen the optimal mixing mass ratio of the two insecticides.

[0076] A. Eggplant thrips (Thrips palmi) were collected from eggplants grown in Hanzhuang, Xiashu Town, Jurong, Zhenjiang City, Jiangsu Province. They had never been exposed to any pesticides and had been reared in clean green beans indoors. The rearing conditions were a temperature of 26±1°C, a relative humidity of 50% to 70%, and a photoperiod of L:D=14:10h. The rearing method was as follows: two green beans were placed in a rearing bottle, 40 female adults were picked in, and they were allowed to lay eggs. After 2 days, the beans were taken out and placed in another new bottle. After they were uniformly hatched into 2nd instar nymphs, the experiment was conducted.

[0077] B. The test agent is 90% methamidin and 90% spinosad technical

[0078] C. The mixing ratio of spinosad: anthracene: is: 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5. The concentrations are shown in Table 3:

[0079] Table 3 The dosage ratio of each substance of spinosad and anthracene insecticide composition

[0080]

[0081] D. Toxicity test method

[0082] Refer to the leaf tube film method of NY / T 3680-2020 Technical Specifications for Monitoring Resistance of Western Flower Thrips. First, heat the tip of the insect needle with an alcohol lamp and make several small holes in the cap and bottom of a 4 cm centrifuge tube. The hole diameter is determined to prevent the thrips from escaping. Then dissolve the test agent directly with acetone, dilute it with 0.1% Tween-80 aqueous solution to the required concentration, and add the solution to the centrifuge tube with a pipette. After shaking upside down several times, pour out the liquid medicine, open the tube cover and place it on the laboratory bench to dry naturally: then cut the fresh eggplant pods into pieces of about 2 cm in length with a knife, immerse them in the test solution for 15 seconds, and then place them on absorbent paper to dry naturally, clamp them into a centrifuge tube, and use a sucker to suck up 15 2-year-old eggplant thrips nymphs and flick them into the centrifuge tube: then place the tube in an artificial climate box with a temperature of (26±1)℃ and a light setting of L:D-14h:10h. The test setting is a 0.1% Tween-80 aqueous solution without the drug (containing the same volume of acetone as the drug treatment) as a blank control. After 48 hours, check the death of the test insects, touch the insect body lightly with the tip of a small brush, and those that do not move are considered dead. Each tube is a replicate, and each concentration is repeated 4 times. The number of deaths is recorded, and the mortality rate and corrected mortality rate are calculated. DPS software is used as a statistical tool to calculate the toxicity regression equation, LD 50 .

[0083] Calculation formula:

[0084]

[0085]

[0086] When the mortality rate of the control drug is <5%, no correction is required. When the mortality rate is between 5% and 20%, correction is performed according to the calibration mortality formula. When the mortality rate is >20%, the test is repeated.

[0087] According to the survey data, the mortality rate and corrected mortality rate of each treatment were calculated, and the DPS software was used for data processing to calculate the LD of each agent. 50 , LD 90 , b value (standard error) and LD 50 The 95% confidence limit of the mixture was calculated according to the Sun Yunpei method.

[0088] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formula (1), formula (2) and formula (3):

[0089]

[0090] Where: ATI—actual toxicity index of the mixture;

[0091] S—LD of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0092] M—LD of the mixture 50 , the unit is milligrams per liter (mg / L).

[0093] TTI=A×P A +B×P B (2)

[0094] Where: TTI—theoretical toxicity index of mixture;

[0095] A—Agent toxicity index;

[0096] P A —The percentage of agent A in the mixture, in percentage (%);

[0097] B—B agent toxicity index;

[0098] P B —The percentage of agent B in the mixture, in percentage (%).

[0099]

[0100] In the formula: CTC—co-toxicity coefficient; ATI—measured toxicity index of the mixture; TTI—theoretical toxicity index of the mixture.

[0101] The co-toxicity coefficient (CTC) of the combination ≥120 indicates a synergistic effect; CTC ≤80 indicates an antagonistic effect; 80 < CTC <120 indicates an additive effect.

[0102] E. Test results Table 4:

[0103] Table 4 Toxicity test results of 9 mixtures of methoxazole and spinosad

[0104]

[0105] The study showed (Table 4) that the effective inhibitory concentration LD of (spinosad:methoxazole = 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5) against eggplant thrips 50They are 0.93, 0.87, 0.82, 0.85, 0.92, 1.02, 1.15, 1.24 and 1.33 mg / L respectively, and the co-toxicity coefficients (CTC) are 114.62, 125.00, 136.77, 139.18, 144.46, 148.63, 141.81, 137.78 and 132.66 respectively; it can be seen that the ratios of spinosad: methoprenaline of 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4 and 1:5 all show synergistic effects; the ratio of spinosad: methoprenaline of 5:1 shows additive effect.

[0106] Experimental Example 3 Field efficacy test

[0107] 1. Test methods

[0108] Field efficacy test 1: The insecticidal compositions prepared in Formulation Example 1 and Comparative Examples 1 and 2 were used for controlling Plutella xylostella in the field.

[0109] The test agents are shown in Table 5 below:

[0110] Table 5 Dosage table for drug test

[0111]

[0112] The experimental field is located in Hanzhuang, Xiashu Town, Jurong, Zhenjiang City, Jiangsu Province (32.178645 N, 119.167816 E, with an area of ​​1.5 mu, soil pH of 6.4, and organic matter content of 1.8%. The experimental field belongs to the northern subtropical monsoon climate zone, with sufficient sunlight, mild climate, distinct four seasons, moderate rainfall, and flat terrain. It has good irrigation and drainage conditions and is suitable for conducting experiments.

[0113] Sowing conditions: Cabbage seeds were purchased from the market. Planting conditions: Manual transplanting on July 7, 2022; open-air planting, plant spacing 25 cm, row spacing 40 cm, single ridge 4 rows, planting volume per mu is about 6,600 plants. Cultivation and management conditions are in line with local agricultural production practices.

[0114] Application time and growth period: The application time is August 11, 2022, when the crops are in the rosette stage and the diamondback moth larvae are in full bloom. Water consumption for application: 450L / hm 2 .

[0115] Investigation on control effect: Method for insect population investigation: Randomly mark 10 cabbages in each plot, conduct an insect population investigation before and after application of pesticides (3d, 7d), record the total number of live larvae, and finally calculate the insect population reduction rate and control effect.

[0116] 2. Calculation method of drug efficacy

[0117] EXCEL was used to calculate the disease index and control effect, and the Duncan new multiple range (DMRT) method was used to perform variance analysis on the test data using DPS9.5 software.

[0118]

[0119]

[0120] 3. Test results

[0121] The control effect of the agent on the lepidopteran pest Plutella xylostella after treatment is shown in Table 6 below.

[0122] Table 6 Control effect of pesticide treatment on cabbage diamondback moth

[0123]

[0124] According to the above table, the results of the control effect investigation show that the dosage of the preparation example 1 is 6 grams per mu; the control effect of the preparation example 1 is 89.13% after 3 days of application, which is significantly better than the control effects of 82.17% and 80.02% of the comparative examples 1 and 2; the control effect of the preparation example 1 is 94.36% after 7 days of application, which is significantly better than the control effects of 85.69% and 82.15% of the comparative examples 1 and 2. According to field observation, the treatment of the above-mentioned examples and comparative examples is safe for the test crop cabbage, and no symptoms of drug damage (such as dwarfing, chlorosis, deformity, etc.) are found.

[0125] Experimental Example 4 Field Efficacy Test

[0126] 1. Test methods

[0127] Field efficacy test 1: The insecticidal compositions prepared in Formulation Example 1 and Comparative Examples 1 and 2 were used for controlling eggplant thrips in the field.

[0128] The test agents are shown in Table 7 below:

[0129] Table 7 Dosage table for drug test

[0130]

[0131] The experimental field is located in Hanzhuang, Xiashu Town, Jurong, Zhenjiang City, Jiangsu Province (32.178645 N, 119.167816 E, with an area of ​​2 mu, soil pH of 6.4, and organic matter content of 1.8%. The experimental field belongs to the northern subtropical monsoon climate zone, with sufficient sunlight, mild climate, distinct four seasons, moderate rainfall, and flat terrain. It has good irrigation and drainage conditions and is suitable for conducting experiments.

[0132] Sowing situation: seeds were purchased from the market and sown on May 25, 2022. The row and plant spacing was 0.25*0.4m, and the cultivation and management conditions were in line with local agricultural production practices.

[0133] Application time and growth period: The application time is August 4, 2022, when eggplant is in the flowering period and thrips are in the early stage of nymphalogenesis. The water consumption for application is 600 L / hm 2 .

[0134] Control effect survey: Control effect survey: The "Z"-shaped five-point sampling method was adopted in the plot, with 2 plants surveyed at each point, a total of 10 plants, and 2 flowers in the middle and upper parts of each plant were fixedly surveyed. A total of 40 flowers were surveyed in each plot, and the number of live insects was counted. The number of insects was surveyed and recorded before and 3 and 7 days after the application of the pesticide, and the insect population reduction rate and control effect were calculated.

[0135] 2. Calculation method of drug efficacy

[0136] EXCEL was used to calculate the disease index and control effect, and the Duncan new multiple range (DMRT) method was used to perform variance analysis on the test data using DPS9.5 software.

[0137]

[0138]

[0139] 3. Test results

[0140] The control effects of different pesticides on eggplant thrips are shown in Table 8 below.

[0141] Table 8 Control effect of the thrips on eggplant after treatment with the pesticide

[0142]

[0143] According to the above table, the results of the control effect investigation show that the control effect of Example 1, the dosage of the preparation is 10 grams per mu; 3 days after the application, the control effect is 92.54%, which is significantly better than the control effects of 85.33% and 84.11% of Comparative Examples 1 and 2; 7 days after the application, the control effect of Example 1 is 97.19%, which is significantly better than the control effects of 88.21% and 86.58% of Comparative Examples 1 and 2. Field observations show that the above test agents and control agents are safe for the test crop eggplant, and no symptoms of drug damage (such as dwarfing, chlorosis, deformity, etc.) were found.

[0144] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A pesticide insecticide composition, characterized in that: These include methoxazole and spinosad; The mass ratio of imipenem to spinosad is (1-30): (1-30); The insecticide composition is used for preventing and controlling insect pests of crops.

2. A pesticide insecticide composition according to claim 1, characterized in that: The mass of the imipenem accounts for 1-30% of the mass of the pesticide; the mass of spinosad accounts for 1-30% of the mass of the pesticide.

3. A pesticide insecticide composition according to claim 2, characterized in that: The mass of the imipenem accounts for 6% of the mass of the pesticide; the mass of the spinosad accounts for 6% of the mass of the pesticide.

4. A pesticide insecticide composition according to claim 2, characterized in that: The formulation of the insecticide composition is a liquid preparation.

5. A pesticide insecticide composition according to claim 1, characterized in that: It also includes auxiliary ingredients; The auxiliary material components include the following by mass percentage: Surfactant, 10-20%; Antifreeze, 1-10%; Solvent, 1-20%; Carrier, 1~50%.

6. A pesticide insecticide composition according to claim 5, characterized in that: The surfactant includes nonylphenol polyoxyethylene ether, nonylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether polyoxypropylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxypropylene polyoxyethylene ether, phenethylphenol polyoxyethylene ether phosphate, benzylphenol polyoxyethylene ether, phenethylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, lauryl alcohol polyoxyethylene ether, fatty amine polyoxyethylene ether, castor oil ethylene oxide adduct, sorbitan fatty acid ester, sorbitan fatty acid ester, One or more of ester ethylene oxide adduct, calcium dodecylbenzene sulfonate, sulfated castor oil, fatty alcohol sulfate, fatty alcohol polyoxyethylene ether sulfate, fatty acid polyoxyethylene ester phosphate, alkylamine polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether formaldehyde condensate, polyvinyl alcohol monooleate, polyethylene glycol monooleate, polyoxyethylene polyoxypropylene block copolymer, polycarboxylate, polyacrylic acid, sodium alkylnaphthalene sulfonate fatty alcohol polyoxyethylene ether, sorbitan monooleate, polyoxyethylene sorbitan monooleate, and castor oil polyoxyethylene ether.

7. A pesticide insecticide composition according to claim 5, characterized in that: The antifreeze agent includes one or more of ethylene glycol, glycerol, propylene glycol, urea, methanol, ethanol, isopropanol, diethylene glycol, ethylene glycol butyl ether, propylene glycol butyl ether, ethylene glycol butyl ether acetate, and isooctyl alcohol.

8. A pesticide insecticide composition according to claim 5, characterized in that: The solvent includes one or more of solvent oil, toluene, xylene, methanol, ethanol, methyl oleate, cyclohexanone, vegetable oil, methyl vegetable oil, dimethylformamide, acetonitrile, polyethylene glycol, caprylic acid amide, tributyl phosphate, butyrolactone, N-long-chain alkyl pyrrolidone, octyl pyrrolidone, and isooctyl alcohol.

9. A pesticide insecticide composition according to claim 1, characterized in that: The carrier is ionized water.

10. Use of a pesticide insecticide composition in preventing and controlling insect pests of crops.

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