Oral care composition as well as preparation method and application thereof

Abstract

The invention discloses an oral care composition as well as a preparation method and application thereof. The oral care composition is prepared from the following components in parts by weight: 0.01 to 5 parts of biological membrane remover, 0.2 to 2 parts of zinc ion bacteriostatic agent, 0.2 to 1 part of tea polyphenol, 0.2 to 1 part of rosemary extract, 0.1 to 2 parts of amylase, 0.1 to 2 parts of protease, 0.01 to 5 parts of repairing agent, 0 to 2 parts of preservative, 0 to 1 part of pH regulator, 0 to 1 part of chelating agent and 80 to 95 parts of water, the biological membrane remover is selected from one or more of yeast fermentation product extract, rhamnolipid and lipopeptide. According to the oral care composition, hydrogen sulfide and methyl mercaptan are removed by inhibiting bacteria in the oral cavity, so that the oral malodor of pets is greatly improved; in addition, the toothpaste can also play a role in improving dental plaque and gingivitis, so that the effects of freshening and protecting teeth are achieved.

Description

Technical Field

[0001] The present invention belongs to the technical field of oral care products, and more specifically, relates to an oral care composition and a preparation method and application thereof. Background Art

[0002] With the booming development of the pet industry, more and more people are keeping pets. The oral health of pets has a significant impact on the quality of life and overall health of pets. The oral health of pets has become a concern for many pet owners and is also widely valued in pet clinical medicine. At present, common oral problems of pets include dental plaque, tartar, gingivitis, periodontitis and other oral diseases, which may cause bad breath, drooling, pain, anorexia and other symptoms in pets, seriously affecting the quality of life of pets.

[0003] Bad breath in pets is the main problem in pet oral care. Among them, hydrogen sulfide (H2S) and methyl mercaptan (CH3SH) play a major role in bad breath. These two compounds account for more than 90% of VSCs in the mouth. They are the two main components of volatile sulfides (VSCs). They are produced by anaerobic bacteria in the mouth through the metabolism of sulfur-containing amino acids. Volatile sulfides are released into the mouth, causing bad breath. Hydrogen sulfide has the smell of rotten eggs and can produce a noticeable odor even at very low concentrations; and methyl mercaptan is also an important component of bad breath, and its odor index is much larger than that of hydrogen sulfide.

[0004] Dental plaque is a biofilm formed by the aggregation of bacteria and their byproducts, saliva components, oral impurities, and a small number of epithelial cells and inflammatory cells. Within a few hours after cleaning teeth, a biofilm will slowly begin to form on the clean surface of teeth. When oral hygiene management is lacking, dental plaque will gradually accumulate and thicken. As dental plaque accumulates, periodontitis pathogens begin to multiply in large numbers, and this process takes less than 24 hours. The accumulation of dental plaque will lead to the formation of dental calculus, which will cause gingivitis in pets.

[0005] Currently, the common solution to pets' bad breath, dental plaque and gingivitis is to solve pets' oral problems by inhibiting the activity of oral microorganisms and cleaning. However, existing antibacterial agents and cleaners are very irritating to pets' oral cavity, making it difficult to remove biofilms, and have little effect on improving pets' bad breath, dental plaque and gingivitis. Summary of the invention

[0006] In view of the above-mentioned existing technical problems, the primary purpose of the present invention is to provide an oral care composition, which can inhibit bacteria in the oral cavity, remove hydrogen sulfide and methyl mercaptan, thereby greatly improving the bad smell in the pet's mouth; in addition, it can also improve dental plaque and gingivitis, thereby achieving the effect of refreshing teeth protection.

[0007] The second object of the present invention is to provide a method for preparing the oral care composition.

[0008] The third object of the present invention is to provide an application of an oral care composition in improving the antibacterial rate, the hydrogen sulfide removal rate and / or the methyl mercaptan removal rate.

[0009] A fourth object of the present invention is to provide a use of an oral care composition in improving the oral odor of an animal.

[0010] A fifth object of the present invention is to provide an oral care composition for use in improving dental plaque and / or gingivitis in animals.

[0011] In order to achieve the above object, the present invention is implemented by the following technical solutions:

[0012] The oral care composition for protection of the present invention comprises, by weight: 0.01-5 parts of a biofilm remover, 0.2-2 parts of a zinc ion antibacterial agent, 0.2-1 parts of tea polyphenols, 0.2-1 parts of a rosemary extract, 0.1-2 parts of an amylase, 0.1-2 parts of a protease, 0.01-5 parts of a repair agent, 0-2 parts of a preservative, 0-1 parts of a pH adjuster, 0-1 parts of a chelating agent, and 80-95 parts of water; the biofilm remover is selected from one or more of a yeast fermentation product extract, rhamnolipids, and lipopeptides.

[0013] Biofilm is formed by the aggregation of bacteria and their byproducts, saliva components, oral impurities and a small number of epithelial cells and inflammatory cells. The formation of biofilm will greatly affect the antibacterial components in the components to inhibit the growth of bacteria such as Streptococcus mutans that cause bad breath in pets' mouths; in addition, it will also affect the cleaning components to decompose food residues in the pet's mouth and reduce the source of odor. The present invention uses yeast fermentation product extract as a biofilm remover, which can not only remove dental plaque by inhibiting the growth of biofilm and destroying biofilm, but also solve oral problems such as bad breath and tartar gingivitis in pets from the root; it can also prevent bacteria that cause bad breath in pets' mouths from attaching to teeth, thereby promoting the specific antibacterial components and cleaning components in the components to play their role.

[0014] Further, the present invention adopts a specific antibacterial agent combination to play a role in synergistically improving the antibacterial rate, hydrogen sulfide removal rate and methyl mercaptan removal rate. Zinc gluconate has good antibacterial activity, zinc ions have an inhibitory effect on oral odor, can directly combine with gaseous hydrogen sulfide and inhibit the growth of oral bacteria that produce volatile sulfides, and has the effect of preventing and treating the formation of dental calculus on the tooth surface. Tea polyphenols not only have a strong antibacterial activity against the main periodontal pathogens, but also have the effect of improving oral inflammation resistance, improving oral inflammation, and reducing the generation of inflammatory factors. Rosemary extract, as a natural antibacterial agent, has a strong antibacterial activity against oral pathogens. Rosmarinic acid can accelerate the repair of oral mucosa, inhibit inflammatory reactions, and has a good antioxidant effect, which can avoid protecting oral tissue from free radical damage.

[0015] Furthermore, the present invention adopts a specific cleaning combination, and the amylase and protease have excellent decomposition effects on food residues in the pet's mouth, thereby cleaning the oral cavity and reducing the source of odor.

[0016] The present invention adopts a specific biofilm remover, and is matched with a zinc ion antibacterial agent, tea polyphenols and rosemary extract, as well as amylase and protease in the system. Through the simultaneous action of three different dimensions, it synergistically improves the antibacterial rate, hydrogen sulfide removal rate and methyl mercaptan removal rate, refreshes the pet's oral odor, and greatly improves the pet's oral odor; in addition, it can also improve dental plaque and gingivitis, thereby achieving the effect of refreshing and protecting teeth.

[0017] Specifically, the amount of the biofilm remover can be 0.05 parts, 0.08 parts, 0.1 parts, 0.5 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, etc., or the interval range formed by any of the above values, such as 0.05-3.5 parts, 1-4.5 parts, etc., the present invention is not limited thereto. Specifically, the amount of the zinc ion antibacterial agent can be 0.4 parts, 0.6 parts, 0.8 parts, 1 parts, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts, etc., or the interval range formed by any of the above values, such as 0.4-1.6 parts, 0.6-0.8 parts, etc., the present invention is not limited thereto. Specifically, the amount of the tea polyphenols can be 0.4 parts, 0.6 parts, 0.8 parts, 1 parts, etc., or the interval range formed by any of the above values, such as 0.4-1 parts, 0.6-0.8 parts, etc., the present invention is not limited thereto. Specifically, the amount of the rosemary extract can be 0.4 parts, 0.6 parts, 0.8 parts, 1 parts, etc., or the interval range formed by any of the above values, such as 0.4-1 parts, 0.6-0.8 parts, etc., and the present invention is not limited thereto. Specifically, the amount of the repair agent can be 0.02 parts, 0.04 parts, 0.06 parts, 0.08 parts, 0.1 parts, 0.15 parts, 0.3 parts, 0.45 parts, 0.6 parts, 0.75 parts, 0.9 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, etc., or the interval range formed by any of the above values, such as 0.04-0.15 parts, 0.06-3 parts, etc., and the present invention is not limited thereto.

[0018] Preferably, the zinc ion antibacterial agent is selected from one or more of zinc gluconate, zinc sulfate and zinc chloride.

[0019] Preferably, the repair agent is selected from one or more of β-glucan, hyaluronic acid, amino acids, plant polypeptides, vitamins, and propolis. Hyaluronic acid has good film-forming properties, isolates pathogens and foreign body stimulation, prevents the spread of inflammatory factors, protects oral mucosa and gingival tissue, and has good transdermal absorption, can repair cell damage, reduce the release of inflammatory factors, and promote the healing of tiny wounds inside the oral cavity.

[0020] Preferably, the preservative is selected from one or more of sodium benzoate, potassium sorbate, sodium dehydroacetate, benzoic acid, lactic acid, propionic acid, and sodium bicarbonate.

[0021] Preferably, the pH regulator is selected from one or more of citric acid, sodium citrate, sodium bicarbonate, lactic acid, and sodium hydroxide.

[0022] Preferably, the chelating agent is selected from one or more of ethylenediaminetetraacetic acid, disodium ethylenediaminetetraacetate, and tetrasodium ethylenediaminetetraacetate.

[0023] Preferably, the oral care composition comprises, by weight: 2-5 parts of a biofilm remover, 0.2-1 parts of a zinc ion antibacterial agent, 0.2-0.5 parts of tea polyphenols, 0.2-0.5 parts of a rosemary extract, 0.1-1 parts of an amylase, 0.1-1 parts of a protease, 0.05-0.5 parts of a repair agent, 0-2 parts of a preservative, 0-1 parts of a pH adjuster, 0-1 parts of a chelating agent, and 80-95 parts of water.

[0024] Preferably, the oral care composition comprises, by weight: 2-5 parts of a biofilm remover, 0.2-1 parts of a zinc ion antibacterial agent, 0.2-0.5 parts of tea polyphenols, 0.2-0.5 parts of a rosemary extract, 0.1-1 parts of an amylase, 0.1-1 parts of a protease, 0.05-0.5 parts of a repair agent, 0.1-1.5 parts of a preservative, 0.01-0.5 parts of a pH adjuster, 0.05-0.5 parts of a chelating agent, and 80-95 parts of water.

[0025] Furthermore, the present invention claims protection for a method for preparing an oral care composition, comprising the following steps: mixing a biofilm remover, a zinc ion antibacterial agent, tea polyphenols, a rosemary extract, an amylase, a protease, a repair agent and other components contained in the system to obtain the oral care composition.

[0026] Furthermore, the present invention claims protection for the use of the oral care composition in improving the antibacterial rate, the hydrogen sulfide removal rate and / or the methyl mercaptan removal rate.

[0027] Furthermore, the present invention claims the use of the oral care composition in improving the oral odor of an animal.

[0028] Furthermore, the present invention claims the use of the oral care composition in improving dental plaque and / or improving gingivitis in animals. More specifically, the oral care composition is particularly suitable for pets, such as cats, dogs, and the like.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The present invention adopts a specific biofilm remover, and is matched with a zinc ion antibacterial agent, tea polyphenols and rosemary extract, as well as amylase and protease in the system. Through the simultaneous action of three different dimensions, it synergistically improves the antibacterial rate, hydrogen sulfide removal rate and methyl mercaptan removal rate, refreshes the pet's oral odor, and greatly improves the pet's oral odor; in addition, it can also improve dental plaque and gingivitis, thereby achieving the effect of refreshing and protecting teeth. DETAILED DESCRIPTION

[0031] The present invention is further described below in conjunction with specific examples of the specification, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0032] The raw material information used in the examples and comparative examples is shown below.

[0033] Yeast ferment extract, BioToLife, BASF.

[0034] Rosemary Extract, Rosemary Extract, Baoyi Bio.

[0035] Amylase, Amplify Prime 100L, Novozymes.

[0036] Proteases, 8000, AB Enzymes.

[0037] Hyaluronic acid, Bloomage Biopharmaceuticals.

[0038] Example 1 Oral Care Composition

[0039] In terms of weight, the oral care composition includes: 3 parts of yeast fermentation product extract, 0.5 parts of zinc gluconate, 0.5 parts of tea polyphenols, 0.5 parts of rosemary extract, 0.2 parts of amylase, 0.2 parts of protease, 0.1 parts of hyaluronic acid, 0.5 parts of sodium benzoate, 0.05 parts of citric acid, 0.1 parts of disodium ethylenediaminetetraacetic acid, and 94.35 parts of deionized water.

[0040] The preparation method of the oral care composition comprises the following steps: adding yeast fermentation product extract, zinc gluconate, tea polyphenols, rosemary extract, amylase, protease, hyaluronic acid, sodium benzoate, citric acid, disodium ethylenediaminetetraacetic acid and deionized water in sequence at room temperature, and stirring at a rotation speed of 250 r / min until transparent and uniform without particles, thereby obtaining the oral care composition.

[0041] Example 2 Oral Care Composition

[0042] In terms of weight, the oral care composition includes: 2 parts of yeast fermentation product extract, 0.2 parts of zinc gluconate, 0.2 parts of tea polyphenols, 0.2 parts of rosemary extract, 0.1 parts of amylase, 0.1 parts of protease, 0.1 parts of hyaluronic acid, 0.5 parts of sodium benzoate, 0.05 parts of citric acid, 0.1 parts of disodium ethylenediaminetetraacetic acid, and 96.45 parts of deionized water.

[0043] The preparation method of this example refers to the preparation method in Example 1.

[0044] Example 3 Oral Care Composition

[0045] The oral care composition includes, by weight: 5 parts of yeast fermentation product extract, 1 part of zinc gluconate, 0.5 parts of tea polyphenols, 0.5 parts of rosemary extract, 1 part of amylase, 1 part of protease, 0.1 parts of hyaluronic acid, 0.5 parts of sodium benzoate, 0.05 parts of citric acid, 0.1 parts of disodium ethylenediaminetetraacetic acid, and 90.2 parts of deionized water.

[0046] The preparation method of this example refers to the preparation method in Example 1.

[0047] Comparative Example 1

[0048] The difference between this comparative example and Example 1 is that the yeast fermentation product extract is not included.

[0049] Comparative Example 2

[0050] The difference between this comparative example and Example 1 is that zinc gluconate, tea polyphenols and rosemary extract are not included.

[0051] Comparative Example 3

[0052] The difference between this comparative example and Example 1 is that amylase and protease are not included.

[0053] Comparative Example 4

[0054] The difference between this comparative example and Example 1 is that hyaluronic acid is not included.

[0055] Comparative Example 5

[0056] The difference between this comparative example and Example 1 is that zinc gluconate is not included, the mass fraction of tea polyphenols is 0.35 parts, and the mass fraction of rosemary extract is 0.85 parts.

[0057] Comparative Example 6

[0058] The difference between this comparative example and Example 1 is that tea polyphenols are not included, the mass fraction of zinc gluconate is 0.6 parts, and the mass fraction of rosemary extract is 0.6 parts.

[0059] Comparative Example 7

[0060] The difference between this comparative example and Example 1 is that the rosemary extract is not included, the mass fraction of zinc gluconate is 0.85 parts, and the mass fraction of tea polyphenols is 0.35 parts.

[0061] Test Example 1

[0062] The test methods of the oral care compositions prepared in the above examples and comparative examples are as follows.

[0063] (1) Inhibition rate: The inhibition rate test was conducted using WS / T 650 to test the inhibition rate of Streptococcus mutans.

[0064] (2) The removal rates of hydrogen sulfide and methyl mercaptan are based on Appendix B of JC / T 1074-2021, with the original solution acting for 24 hours.

[0065] The specific test results are shown in Table 1.

[0066] Table 1

[0067]

[0068] As can be seen from Table 1 above, the oral care composition provided by the present invention has excellent antibacterial rate, hydrogen sulfide removal rate and methyl mercaptan removal rate. The oral care composition provided by the present invention has an inhibition rate of ≥99.4% for Streptococcus mutans; a hydrogen sulfide removal rate of ≥98.9%; and a methyl mercaptan removal rate of ≥97.5%. Further preferably, the inhibition rate of Streptococcus mutans is ≥99.99%; the hydrogen sulfide removal rate is ≥99.8%; and the methyl mercaptan removal rate is ≥99.5%.

[0069] It can be seen from Example 1, Comparative Example 1, Comparative Example 2 and Comparative Example 3 that when the oral care composition lacks the yeast fermentation product extract; or lacks zinc gluconate, tea polyphenols and rosemary extract; or lacks amylase and protease, its inhibition rate on Streptococcus mutans is significantly reduced. In addition, the hydrogen sulfide removal rate and methyl mercaptan removal rate are far from those in Example 1, and it is difficult to achieve the technical effects of the present invention.

[0070] It can be seen from Example 1, Comparative Example 5, Comparative Example 6 and Comparative Example 7 that when zinc gluconate, tea polyphenols and rosemary extract are used in combination in the oral care composition, the hydrogen sulfide removal rate and methyl mercaptan removal rate are significantly improved when the three are combined compared with the combination of any two.

[0071] Test Example 2: Effect of improving pet oral odor

[0072] Test method: 10 pets (5 cats, 5 dogs) use the oral care compositions prepared in the examples and comparative examples, and 5 people smell the oral odor of the pets before and after using the oral care compositions, and compare them. The improvement effect of the oral odor of the pets is scored, and the score is the average score. Among them, 0-1 points correspond to no odor; 1-2 points correspond to very light odor; 2-3 points correspond to light odor; 3-4 points correspond to moderate odor; 4-5 points correspond to strong odor; 5 points correspond to very strong odor. The specific test results are shown in Table 2.

[0073] Table 2

[0074]

[0075] It can be seen from Table 2 above that after the pets used the oral care composition of the present invention, the oral odor scores were all ≤0.6.

[0076] It can be seen from Example 1, Comparative Examples 1 to 3, and Comparative Examples 5 to 7 that the oral care composition lacks the yeast fermentation product extract, and the oral odor score is between 1-2; lacks zinc gluconate, tea polyphenols and rosemary extract, and the oral odor score is between 3-4; using any two combinations of zinc gluconate, tea polyphenols and rosemary extract, the oral odor score is between 1-3; lacks amylase and protease, and the oral odor score is between 1-2, and it is difficult to achieve the technical effects of the present invention.

[0077] Test Example 3: Improvement of dental plaque and gingivitis in pets' oral cavity

[0078] Test method: 5 pets were given oral care compositions of the embodiment and comparative example twice a day for 1 week, and the oral condition was observed and scored as the average score. Among them, 0-1 points correspond to no significant improvement; 1-2 points correspond to slight improvement; 3-4 points correspond to relatively significant improvement; 4-5 points correspond to significant improvement. The specific test results are shown in Table 3.

[0079] Table 3

[0080]

[0081] It can be seen from Table 3 that after the pets used the oral care composition provided by the present invention, the scores of dental plaque and gingivitis were both 4-5 points, and both dental plaque and gingivitis were significantly improved.

[0082] As can be seen from Comparative Examples 1 to 3, when the oral care composition lacks yeast fermentation product extract; or lacks zinc gluconate, tea polyphenols and rosemary extract; or lacks amylase and protease, the scores of dental plaque and gingivitis are between 2 and 4 points, which is only a significant improvement. As can be seen from Comparative Example 4, when the system lacks hyaluronic acid, its improvement score for gingivitis is only 2.5, which is only a slight improvement.

[0083] The foregoing examples are merely illustrative and are used to explain some features of the method of the present invention. The appended claims are intended to require the widest possible range that can be imagined, and the embodiments presented herein are demonstrated by the applicant's actual test results. Therefore, the applicant's intention is that the appended claims are not limited by the selection of examples that illustrate the features of the present invention. Some numerical ranges used in the claims also include sub-ranges therein, and changes in these ranges should also be interpreted as being covered by the appended claims where possible.

Claims

1. An oral care composition, characterized in that According to weight parts, it includes: 0.01-5 parts of biofilm remover, 0.2-2 parts of zinc ion antibacterial agent, 0.2-1 parts of tea polyphenols, 0.2-1 parts of rosemary extract, 0.1-2 parts of amylase, 0.1-2 parts of protease, 0.01-5 parts of repair agent, 0-2 parts of preservative, 0-1 parts of pH adjuster, 0-1 parts of chelating agent, and 80-95 parts of water; The biofilm remover is selected from one or more of yeast fermentation product extract, rhamnolipid, and lipopeptide.

2. The oral care composition according to claim 1, characterized in that The repair agent is selected from one or more of β-glucan, hyaluronic acid, amino acids, plant polypeptides, vitamins, and propolis.

3. The oral care composition according to claim 1, characterized in that The preservative is selected from one or more of sodium benzoate, potassium sorbate, sodium dehydroacetate, benzoic acid, lactic acid, propionic acid, and sodium bicarbonate.

4. The oral care composition according to claim 1, characterized in that: The pH regulator is selected from one or more of citric acid, sodium citrate, sodium bicarbonate, lactic acid, and sodium hydroxide.

5. The oral care composition according to claim 1, characterized in that: The chelating agent is selected from one or more of ethylenediaminetetraacetic acid, disodium ethylenediaminetetraacetate, and tetrasodium ethylenediaminetetraacetate.

6. The oral care composition according to claim 1, characterized in that: include: 2-5 parts of biofilm remover, 0.2-1 parts of zinc ion antibacterial agent, 0.2-0.5 parts of tea polyphenols, 0.2-0.5 parts of rosemary extract, 0.1-1 parts of amylase, 0.1-1 parts of protease, 0.05-0.5 parts of repair agent, 0-2 parts of preservative, 0-1 parts of pH adjuster, 0-1 parts of chelating agent, and 80-95 parts of water.

7. The method for preparing the oral care composition according to any one of claims 1 to 6, characterized in that: The following steps are involved: The biofilm remover, the zinc ion antibacterial agent, the tea polyphenols, the rosemary extract, the amylase, the protease, the repair agent and other components contained in the system are mixed to obtain the oral care composition.

8. Use of the oral care composition according to any one of claims 1 to 6 in improving the antibacterial rate, hydrogen sulfide removal rate and / or methyl mercaptan removal rate.

9. Use of the oral care composition according to any one of claims 1 to 6 in improving the oral odor of animals.

10. Use of the oral care composition according to any one of claims 1 to 6 in improving dental plaque and / or gingivitis in animals.

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