Collagen cosmetic with moisturizing effect and preparation method thereof

Abstract

The invention discloses a collagen cosmetic with a moisturizing effect and a preparation method thereof. The collagen cosmetic is prepared from the following raw materials: 5 to 10 parts of collagen peptide, 1 to 5 parts of hyaluronic acid, 3 to 8 parts of glycerinum, 1 to 6 parts of vitamin C liquid, 2 to 4 parts of herba centellae extract, 2 to 6 parts of ceramide, 0.1 to 0.5 part of ergothioneine and 5 to 10 parts of aloe juice. The moisturizing component composed of collagen peptide, hyaluronic acid and glycerin can provide sufficient moisture and moist for skin, and the antioxidant component composed of vitamin C liquid and ergosulfide can protect the skin from being damaged by free radicals and promote metabolism and renewal of the skin. The anti-inflammatory and repairing component composed of the centella asiatica extract and ceramide can relieve sensitivity and discomfort of skin and enhance the barrier function of the skin, and the aloe juice can further moisten and relieve the skin, so that the cosmetic not only has remarkable moisturizing and whitening effects, but also can be suitable for people with sensitive skin.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to a collagen cosmetic with a moisturizing effect and a preparation method thereof. Background Art

[0002] There are many cosmetics containing collagen ingredients on the market, such as creams, essences and sprays. Collagen is a biological polymer substance and the most abundant protein in the human body, accounting for about 25%-33% of the total protein in the human body. It weaves into an elastic fiber network in the skin, providing elasticity and firmness to the skin, and plays the role of a deep reservoir, providing moisture to the epidermis, with a super moisturizing effect.

[0003] For example, the patent application number is 202310891483.8, which discloses a collagen peptide cosmetic and its preparation method. The collagen peptide cosmetic includes the following components by weight: 3-5 parts of collagen peptide, 5-10 parts of modified chitosan, 8-15 parts of moisturizer, 6-8 parts of emulsifier, 0.1-0.2 parts of preservative, 3-5 parts of thickener, 3-4 parts of pH regulator, and 50 parts of water. The modified chitosan is chitosan modified with kojic acid, and collagen peptides are introduced by grafting copolymerization. The invention proposes kojic acid-modified chitosan, grafting chlorokojic acid ester on the primary hydroxyl group at the C-6 position and the secondary hydroxyl group at the C-3 position of the chitosan molecular chain, and then grafting collagen peptides on the C-2 amino group of the kojic acid-modified chitosan molecular chain, and then introducing collagen peptides and chlorokojic acid esters with moisturizing and whitening effects into the formula, and finally achieving the technical effect of improving the moisturizing and whitening of cosmetics.

[0004] However, since the above-mentioned cosmetics contain modified chitosan, and this modified chitosan is chitosan modified by kojic acid, although it is safe to control the amount of kojic acid added in cosmetics to be less than 2%, excessive addition may cause skin allergies and dermatitis, and is not suitable for use by people with sensitive skin, which limits the scope of use of the cosmetics. Therefore, we need to propose a collagen cosmetic with a moisturizing effect and a preparation method thereof to solve the above-mentioned problems, so that it can have a moisturizing and whitening effect while also being suitable for use by people with sensitive skin, thereby increasing the scope of use of the cosmetics. Summary of the invention

[0005] The purpose of the present invention is to provide a collagen cosmetic with a moisturizing effect and a preparation method thereof, which can have a moisturizing and whitening effect and can also be suitable for use by people with sensitive skin, thereby increasing the scope of use of cosmetics and solving the problems raised in the above-mentioned background technology.

[0006] To achieve the above object, the present invention adopts the following technical solutions:

[0007] A collagen cosmetic with a moisturizing effect comprises the following raw materials, calculated by mass: 5-10 parts of collagen peptide, 1-5 parts of hyaluronic acid, 3-8 parts of glycerin, 1-6 parts of vitamin C liquid, 2-4 parts of Centella asiatica extract, 2-6 parts of ceramide, 0.1-0.5 parts of ergothioneine, and 5-10 parts of aloe juice.

[0008] Preferably, the following raw materials are included by mass: 5 parts of collagen peptide, 1 part of hyaluronic acid, 3 parts of glycerol, 1 part of vitamin C liquid, 2 parts of Centella asiatica extract, 2 parts of ceramide, 0.1 part of ergothioneine, and 5 parts of aloe juice.

[0009] Preferably, the following raw materials are included by mass: 8 parts of collagen peptide, 3 parts of hyaluronic acid, 5 parts of glycerin, 3 parts of vitamin C liquid, 3 parts of Centella asiatica extract, 4 parts of ceramide, 0.3 parts of ergothioneine, and 8 parts of aloe juice.

[0010] Preferably, the preparation process of the Centella asiatica extract is as follows:

[0011] A1. Collect fresh Centella asiatica. Wash and dry the collected Centella asiatica.

[0012] A2. Crush the dried Centella asiatica;

[0013] A3. Add the crushed powder into a 75% pure ethanol solution and soak for 1-3 hours at a soaking temperature of 30°C-45°C;.

[0014] A4, filtering the extract and collecting the filtrate, and concentrating the filtrate to 40%-60% of the total volume to obtain a concentrated filtrate;

[0015] A5, use macroporous adsorption resin d101 macroporous adsorption resin for adsorption, then elute and collect the eluate;

[0016] A6. Further concentrating the separated and purified extract;

[0017] A7. The concentrated extract is dried by freeze drying.

[0018] Preferably, the preparation process of described ergothioneine is as follows:

[0019] B1. Using mushroom mycelium fermentation liquid as raw material, collecting mycelium by solid-liquid separation, and adding water to the collected mycelium at a ratio of 1:0.5-40 to prepare a mycelium water suspension;

[0020] B2, the mycelium aqueous suspension is warming up to 70 ℃ -100 ℃, with the rotating speed stirring and leaching 30-60 minute of 200-600rpm, again carries out solid-liquid separation, collects mycelium and thioneine aqueous solution respectively;

[0021] B3, the thioneine aqueous solution is ultrafiltered, and the molecular weight cut-off of ultrafiltration membrane is between 1kDa-30kDa;

[0022] B4, collecting the ultrafiltration liquid, and drying or concentrating it to obtain a dry crude product or a concentrated liquid;

[0023] B5. Add 1-5 times the volume of acetonitrile to the dry crude product or concentrated solution and carry out ultrasonic treatment. Concentrate after filtering to condense into thioneine solid.

[0024] Preferably, the preparation process of the ceramide is as follows:

[0025] C1. Inoculate Pichia pastoris or Saccharomyces cerevisiae in a culture medium for 2-3 days. The culture medium contains glucose and amino acids. The pH of the culture medium is 5.0-6.0 and the temperature is 25°C-30°C.

[0026] C2, filtering the cultured solution to obtain a filtrate containing ceramide;

[0027] C3, centrifugally filtering the filtrate to obtain a filtration product;

[0028] C4. Purify and dry the filtered product to obtain pure ceramide powder.

[0029] Preferably, the preparation process of the hyaluronic acid is as follows:

[0030] D1. Prepare equal volumes of streptococci, lactic acid bacteria, enterococci and bacillus and mix them to obtain microbial strains;

[0031] D2. Inoculate the microbial strain into the culture medium, then add glucose and yeast powder into the culture medium for fermentation culture, the ratio of the microbial strain to glucose and yeast powder is 1:2.5:0.2;

[0032] D3. After 6-7 days of cultivation, the fermented product is filtered to remove mycelium and impurities;

[0033] D4. Heat and stir the filtered solution with distilled water at a temperature of 120°C-130°C for 25-35 minutes;

[0034] D5. The hydrolyzed solution is sequentially centrifuged, concentrated and dried to obtain high-purity hyaluronic acid.

[0035] Preferably, the preparation process of the aloe juice is as follows:

[0036] E1. Choose aloe vera that is free of pests and diseases, has full leaves and bright green color. Wash the aloe vera leaves with clean water to remove dust and impurities on the surface.

[0037] E2. Use a knife or peeler to remove the skin and thorns of the aloe leaves;

[0038] E3. Cut the processed aloe leaves into small sections or strips;

[0039] E4. Put the aloe vera pulp into a juicer and add purified water in a 1:1 ratio to squeeze the juice. After squeezing the juice, use a filter to remove impurities and fibers in the aloe vera juice to obtain pure aloe vera juice.

[0040] Preferably, the preparation process of the collagen peptide is as follows:

[0041] F1, select dry fish scales and fish skin, and crush them to obtain raw material powder;

[0042] F2. Add an enzymatic agent to the raw material powder for shearing. The enzymatic agent is papain or pepsin to decompose it into small molecular peptides. The enzymatic hydrolysis temperature is 90°C-100°C, the pH value is 6-8, the enzymatic hydrolysis time is 2-6 hours, and the enzyme addition amount is 4000U / g;

[0043] F3, mixing activated carbon particles in the enzymatic solution to remove pigments and odors in the solution;

[0044] F4, centrifuging the mixed solution to remove impurities and suspended matter in the solution to obtain a liquid solution;

[0045] F5. Further filter the liquid solution using a microfiltration or ultrafiltration membrane to remove impurities such as macromolecular proteins, polypeptides and salts in the solution to obtain a secondary filtrate;

[0046] F6, concentrating the secondary filtrate;

[0047] F7. Freeze-dry the concentrated collagen peptide solution to obtain a powdered or granular collagen peptide product.

[0048] Based on the above-described collagen cosmetics with moisturizing effect, the present invention also provides a method for preparing the collagen cosmetics with moisturizing effect, comprising the following steps:

[0049] S1. Prepare the following raw materials according to the mass component ratio: collagen peptide, hyaluronic acid, glycerin, vitamin C liquid, Centella asiatica extract, ceramide, ergothioneine and aloe vera juice;

[0050] S2, adding collagen peptide, hyaluronic acid, glycerin, vitamin C liquid, Centella asiatica extract, ceramide, ergothioneine and aloe vera juice into a constant temperature container at a temperature of 40° C.-80° C., stirring and mixing, so that the solid raw materials are fully dissolved to obtain a mixed solution;

[0051] S3, adding an emulsifier to the mixed solution for emulsification, wherein the emulsifier is set to be one of lanolin alcohol, lanolin acid and lanolin, and the emulsification temperature is between 40° C. and 80° C.;

[0052] S4, using a homogenizing device to refine the emulsified mixture;

[0053] S5. Cool the refined emulsion to room temperature and then package and store it.

[0054] The collagen cosmetics with moisturizing effect and the preparation method thereof proposed by the present invention have the following advantages compared with the prior art:

[0055] 1. The cosmetics of the present invention mainly use collagen peptide, hyaluronic acid, glycerin, vitamin C liquid, Centella asiatica extract, ceramide, ergothioneine and aloe juice as raw materials, wherein the moisturizing component composed of collagen peptide, hyaluronic acid and glycerin can provide sufficient moisture and moisturizing for the skin, the antioxidant component composed of vitamin C liquid and ergothioneine can protect the skin from free radical damage, promote skin metabolism and renewal, the anti-inflammatory and repairing component composed of Centella asiatica extract and ceramide can relieve skin sensitivity and discomfort, enhance the barrier function of the skin, and aloe juice can further moisturize and soothe the skin, so that the cosmetics not only have significant hydrating and whitening effects, but also can be suitable for people with sensitive skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0056] Figure 1 This is a flowchart of the cosmetic preparation process of the present invention;

[0057] Figure 2 The figure is a flowchart of the preparation process of the Centella asiatica extract of the present invention;

[0058] Figure 3 It is a preparation flow chart of ergothioneine of the present invention;

[0059] Figure 4 is a flowchart of the preparation process of ceramide of the present invention;

[0060] Figure 5 is a flowchart of the preparation process of hyaluronic acid of the present invention;

[0061] Figure 6 The present invention is a flowchart of the preparation process of aloe juice;

[0062] Figure 7 The figure is a flowchart of the preparation process of the collagen peptide of the present invention. DETAILED DESCRIPTION

[0063] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. The specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0064] Example 1

[0065] The present invention provides a collagen cosmetic with a moisturizing effect, which comprises the following raw materials by weight: 5 parts of collagen peptide, 1 part of hyaluronic acid, 3 parts of glycerol, 1 part of vitamin C liquid, 2 parts of Centella asiatica extract, 2 parts of ceramide, 0.1 part of ergothioneine, and 5 parts of aloe juice;

[0066] like Figure 2 As shown, the preparation process of the Centella asiatica extract is as follows:

[0067] A1. Collect fresh Centella asiatica and make sure it is in the vegetative stage, plump and not damaged by pests and diseases. The collected Centella asiatica should be cleaned and dried for subsequent processing;

[0068] A2. Grinding the dried Centella asiatica is beneficial to the dissolution of the active ingredients;

[0069] A3. Add the crushed powder into a 75% pure ethanol solution and soak for 1-3 hours at a soaking temperature of 30°C-45°C;.

[0070] A4, filtering the extract and collecting the filtrate, and concentrating the filtrate to 40%-60% of the total volume to obtain a concentrated filtrate;

[0071] A5. Use macroporous adsorption resin d101 for adsorption, then elute, collect the eluate, so as to effectively separate the effective components in Centella asiatica;

[0072] A6. Further concentrating the separated and purified extract to improve the purity and concentration of the extract;

[0073] A7. Drying the concentrated extract by freeze drying to retain the active ingredients in the extract to the greatest extent;

[0074] Soothes sensitive skin with Centella Asiatica Extract's anti-inflammatory, antioxidant and restorative properties.

[0075] like Figure 3 Shown, the preparation process of described thioneine is as follows:

[0076] B1. Using mushroom mycelium fermentation liquid as raw material, collecting mycelium by solid-liquid separation, and adding water to the collected mycelium at a ratio of 1:0.5-40 to prepare a mycelium water suspension;

[0077] B2, the mycelium aqueous suspension is warming up to 70 ℃ -100 ℃, with the rotating speed stirring and leaching 30-60 minute of 200-600rpm, again carries out solid-liquid separation, collects mycelium and thioneine aqueous solution respectively;

[0078] B3, the thioneine aqueous solution is ultrafiltered, and the molecular weight cut-off of ultrafiltration membrane is between 1kDa-30kDa;

[0079] B4, collecting the ultrafiltration liquid, and drying or concentrating it to obtain a dry crude product or a concentrated liquid;

[0080] B5, in dry crude product or concentrated solution, add 1-5 times of volume of acetonitrile and carry out ultrasonic treatment, concentrate after filtering, and be condensed into thioneine solid;

[0081] Ergothioneine is used to stably and efficiently eliminate various free radicals and delay skin aging.

[0082] like Figure 4 As shown, the preparation process of the ceramide is as follows:

[0083] C1. Inoculate Pichia pastoris or Saccharomyces cerevisiae in a culture medium for 2-3 days. The culture medium contains glucose and amino acids. The pH of the culture medium is 5.0-6.0 and the temperature is 25°C-30°C.

[0084] C2, filtering the cultured solution to obtain a filtrate containing ceramide;

[0085] C3, centrifugally filtering the filtrate to obtain a filtration product;

[0086] C4, purifying and drying the filtered product to obtain pure ceramide powder;

[0087] Ceramide is used to enhance the skin barrier function and reduce the damage to the skin caused by external stimuli.

[0088] like Figure 5 As shown, the preparation process of the hyaluronic acid is as follows:

[0089] D1. Prepare equal volumes of streptococci, lactic acid bacteria, enterococci and bacillus and mix them to obtain microbial strains;

[0090] D2. Inoculate the microbial strain into the culture medium, then add glucose and yeast powder into the culture medium for fermentation culture, the ratio of the microbial strain to glucose and yeast powder is 1:2.5:0.2;

[0091] D3. After 6-7 days of cultivation, the fermented product is filtered to remove mycelium and impurities;

[0092] D4. Heat and stir the filtered solution with distilled water at a temperature of 120°C-130°C for 25-35 minutes to release the hyaluronic acid from the tissue;

[0093] D5. The hydrolyzed solution is sequentially centrifuged, concentrated and dried to obtain high-purity hyaluronic acid;

[0094] Hyaluronic acid absorbs and retains a large amount of moisture, improving the skin barrier function.

[0095] like Figure 6 As shown, the preparation process of the aloe juice is as follows:

[0096] E1. Choose aloe vera that is free of pests and diseases, has full leaves and bright green color. Wash the aloe vera leaves with clean water to remove dust and impurities on the surface.

[0097] E2. Use a knife or peeler to remove the skin and thorns of the aloe vera leaves to prevent the remaining skin and thorns from irritating the skin;

[0098] E3. Cut the processed aloe vera leaves into small pieces or strips for easy juicing;

[0099] E4. Put the aloe vera pulp into a juicer and add purified water in a 1:1 ratio to squeeze the juice. After squeezing the juice, use a filter to remove impurities and fibers in the aloe vera juice to obtain pure aloe vera juice. Use aloe vera juice to increase the skin's moisturizing, anti-inflammatory and repair effects and reduce skin allergic reactions.

[0100] like Figure 7 As shown, the preparation process of the collagen peptide is as follows:

[0101] F1, select dry fish scales and fish skin, and crush them to obtain raw material powder;

[0102] F2. Add an enzymatic agent to the raw material powder for shearing. The enzymatic agent is papain or pepsin to decompose it into small molecular peptides. The enzymatic hydrolysis temperature is 90°C-100°C, the pH value is 6-8, the enzymatic hydrolysis time is 2-6 hours, and the enzyme addition amount is 4000U / g;

[0103] F3, mixing activated carbon particles in the enzymatic solution to remove pigments and odors in the solution;

[0104] F4, centrifuging the mixed solution to remove impurities and suspended matter in the solution to obtain a liquid solution;

[0105] F5. Further filter the liquid solution using a microfiltration or ultrafiltration membrane to remove impurities such as macromolecular proteins, polypeptides and salts in the solution to obtain a secondary filtrate;

[0106] F6. Concentrate the secondary filtrate to improve the concentration and stability of the product. The concentration methods include membrane concentration and evaporation concentration.

[0107] F7. freeze-drying the concentrated collagen peptide solution to obtain a powdered or granular collagen peptide product;

[0108] Replenish collagen to the skin through collagen peptides, promote skin elasticity and reduce wrinkles.

[0109] Glycerin is a hygroscopic moisturizer that can absorb moisture from the air and keep the skin moist;

[0110] Vitamin C liquid is made by crushing medicinal vitamin C tablets, dissolving them in pure water, and filtering out the dissolved particulate impurities. Vitamin C is highly stable and has antioxidant and whitening effects.

[0111] The cosmetics are mainly made of collagen peptides, hyaluronic acid, glycerin, vitamin C liquid, Centella asiatica extract, ceramide, ergothioneine and aloe vera juice. Among them, the moisturizing ingredients composed of collagen peptides, hyaluronic acid and glycerin can provide sufficient moisture and moisturizing for the skin, the antioxidant ingredients composed of vitamin C liquid and ergothioneine can protect the skin from damage by free radicals and promote skin metabolism and renewal, the anti-inflammatory and repair ingredients composed of Centella asiatica extract and ceramide can relieve skin sensitivity and discomfort and enhance the skin's barrier function, and aloe vera juice can further moisturize and soothe the skin, making this cosmetics not only have significant hydrating and whitening effects, but also suitable for people with sensitive skin.

[0112] Based on the above-described collagen cosmetics with moisturizing effect, the present invention also provides a method for preparing the collagen cosmetics with moisturizing effect, such as Figure 1 As shown, the following steps are included:

[0113] S1. Prepare the following raw materials according to the mass component ratio: collagen peptide, hyaluronic acid, glycerin, vitamin C liquid, Centella asiatica extract, ceramide, ergothioneine and aloe vera juice;

[0114] S2, adding collagen peptide, hyaluronic acid, glycerin, vitamin C liquid, Centella asiatica extract, ceramide, ergothioneine and aloe vera juice into a constant temperature container at a temperature of 40° C.-80° C., stirring and mixing, so that the solid raw materials are fully dissolved to obtain a mixed solution;

[0115] S3, adding an emulsifier to the mixed solution for emulsification, wherein the emulsifier is set to be one of lanolin alcohol, lanolin acid and lanolin, and the emulsification temperature is between 40° C. and 80° C.;

[0116] S4. Use homogenization equipment to refine the emulsified mixture to reduce the droplet size and improve the fineness and stability of the cosmetics;

[0117] S5. Cool the refined emulsion to room temperature and then package and store it.

[0118] Example 2

[0119] The similarities are not repeated here. The difference from Example 1 is that the following raw materials are included by mass: 8 parts of collagen peptide, 3 parts of hyaluronic acid, 5 parts of glycerol, 3 parts of vitamin C liquid, 3 parts of Centella asiatica extract, 4 parts of ceramide, 0.3 parts of ergothioneine, and 8 parts of aloe juice.

[0120] Example 3

[0121] The similarities are not repeated here. The difference from Example 1 is that the following raw materials are included by mass: 10 parts of collagen peptide, 5 parts of hyaluronic acid, 8 parts of glycerol, 6 parts of vitamin C liquid, 4 parts of Centella asiatica extract, 6 parts of ceramide, 0.5 parts of ergothioneine, and 10 parts of aloe juice.

[0122] Example 4

[0123] The cosmetics prepared according to the ratios provided in Examples 1, 2 and 3 were compared with the cosmetics in the prior art (named as the control example) for verification (the collagen peptide cosmetics used in the prior art include the following components in parts by weight: 3-5 parts of collagen peptide, 5-10 parts of modified chitosan, 8-15 parts of moisturizer, 6-8 parts of emulsifier, 0.1-0.2 parts of preservative, 3-5 parts of thickener, 3-4 parts of pH regulator, and 50 parts of water). The verification process and results are as follows:

[0124] 24 adults aged 30-40 years old with similar skin conditions were selected and randomly assigned to Example 1, Example 2, Example 3 and a control group of 6 people, and the cosmetics prepared by Example 1, Example 2, Example 3 and the control group were used for effect verification. The verification results are shown in the following table:

[0125]

[0126]

[0127]

[0128] It can be seen from the data in the above table that the moisturizing effect of the cosmetics in Example 1, Example 2 and Example 3 is significantly improved compared with the control example, and the moisturizing time and the stratum corneum water content retention time are relatively long, the skin brightness value is also significantly increased, and with the passage of time, the skin brightness value change rate is small, and it has a certain whitening effect. Therefore, the cosmetics provided by the present invention can have moisturizing and whitening effects, and can also be suitable for use by people with sensitive skin, thereby increasing the scope of use of cosmetics.

[0129] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A collagen cosmetic with moisturizing effect, characterized in that: The composition includes the following raw materials by mass: 5-10 parts of collagen peptide, 1-5 parts of hyaluronic acid, 3-8 parts of glycerin, 1-6 parts of vitamin C liquid, 2-4 parts of Centella asiatica extract, 2-6 parts of ceramide, 0.1-0.5 parts of ergothioneine and 5-10 parts of aloe juice.

2. The collagen cosmetic with moisturizing effect according to claim 1, characterized in that: The components by mass include the following raw materials: 5 parts of collagen peptide, 1 part of hyaluronic acid, 3 parts of glycerin, 1 part of vitamin C liquid, 2 parts of Centella asiatica extract, 2 parts of ceramide, 0.1 part of ergothioneine, and 5 parts of aloe vera juice.

3. The collagen cosmetic with moisturizing effect according to claim 1, characterized in that: The composition comprises the following raw materials by mass: 8 parts of collagen peptide, 3 parts of hyaluronic acid, 5 parts of glycerin, 3 parts of vitamin C liquid, 3 parts of Centella asiatica extract, 4 parts of ceramide, 0.3 parts of ergothioneine and 8 parts of aloe vera juice.

4. The collagen cosmetic with moisturizing effect according to claim 1, characterized in that: The preparation process of the Centella asiatica extract is as follows: A1. Collect fresh Centella asiatica. Wash and dry the collected Centella asiatica. A2. Crush the dried Centella asiatica; A3. Add the crushed powder into a 75% pure ethanol solution and soak for 1-3 hours at a soaking temperature of 30°C-45°C;. A4, filtering the extract and collecting the filtrate, and concentrating the filtrate to 40%-60% of the total volume to obtain a concentrated filtrate; A5, use macroporous adsorption resin d101 macroporous adsorption resin for adsorption, then elute and collect the eluate; A6. Further concentrating the separated and purified extract; A7. The concentrated extract is dried by freeze drying.

5. The collagen cosmetic with moisturizing effect according to claim 4, characterized in that: The preparation process of described ergothioneine is as follows: B1. Using mushroom mycelium fermentation liquid as raw material, collecting mycelium by solid-liquid separation, and adding water to the collected mycelium at a ratio of 1:0.5-40 to prepare a mycelium water suspension; B2, the mycelium aqueous suspension is warming up to 70 ℃ -100 ℃, with the rotating speed stirring and leaching 30-60 minute of 200-600rpm, again carries out solid-liquid separation, collects mycelium and thioneine aqueous solution respectively; B3, the thioneine aqueous solution is ultrafiltered, and the molecular weight cut-off of ultrafiltration membrane is between 1kDa-30kDa; B4, collecting the ultrafiltration liquid, and drying or concentrating it to obtain a dry crude product or a concentrated liquid; B5. Add 1-5 times the volume of acetonitrile to the dry crude product or concentrated solution and carry out ultrasonic treatment. Concentrate after filtering to condense into thioneine solid.

6. The collagen cosmetic with moisturizing effect according to claim 5, characterized in that: The preparation process of the ceramide is as follows: C1. Inoculate Pichia pastoris or Saccharomyces cerevisiae in a culture medium for 2-3 days. The culture medium contains glucose and amino acids. The pH of the culture medium is 5.0-6.0 and the temperature is 25°C-30°C. C2, filtering the cultured solution to obtain a filtrate containing ceramide; C3, centrifugally filtering the filtrate to obtain a filtration product; C4. Purify and dry the filtered product to obtain pure ceramide powder.

7. The collagen cosmetic with moisturizing effect according to claim 6, characterized in that: The preparation process of the hyaluronic acid is as follows: D1. Prepare equal volumes of streptococci, lactic acid bacteria, enterococci and bacillus and mix them to obtain microbial strains; D2. Inoculate the microbial strain into the culture medium, then add glucose and yeast powder into the culture medium for fermentation culture, the ratio of the microbial strain to glucose and yeast powder is 1:2.5:0.2; D3. After 6-7 days of cultivation, the fermented product is filtered to remove mycelium and impurities; D4. Heat and stir the filtered solution with distilled water at a temperature of 120°C-130°C for 25-35 minutes; D5. The hydrolyzed solution is sequentially centrifuged, concentrated and dried to obtain high-purity hyaluronic acid.

8. The collagen cosmetic with moisturizing effect according to claim 7, characterized in that: The preparation process of the aloe juice is as follows: E1. Choose aloe vera that is free of pests and diseases, has full leaves and bright green color. Wash the aloe vera leaves with clean water to remove dust and impurities on the surface. E2. Use a knife or peeler to remove the skin and thorns of the aloe leaves; E3. Cut the processed aloe leaves into small sections or strips; E4. Put the aloe vera pulp into a juicer and add purified water in a 1:1 ratio to squeeze the juice. After squeezing the juice, use a filter to remove impurities and fibers in the aloe vera juice to obtain pure aloe vera juice.

9. The collagen cosmetic with moisturizing effect according to claim 8, characterized in that: The preparation process of the collagen peptide is as follows: F1, select dry fish scales and fish skin, and crush them to obtain raw material powder; F2. Add an enzymatic agent to the raw material powder for shearing. The enzymatic agent is papain or pepsin to decompose it into small molecular peptides. The enzymatic hydrolysis temperature is 90°C-100°C, the pH value is 6-8, the enzymatic hydrolysis time is 2-6 hours, and the enzyme addition amount is 4000U / g; F3, mixing activated carbon particles in the enzymatic solution to remove pigments and odors in the solution; F4, centrifuging the mixed solution to remove impurities and suspended matter in the solution to obtain a liquid solution; F5. Further filter the liquid solution using a microfiltration or ultrafiltration membrane to remove impurities such as macromolecular proteins, polypeptides and salts in the solution to obtain a secondary filtrate; F6, concentrating the secondary filtrate; F7. Freeze-dry the concentrated collagen peptide solution to obtain a powdered or granular collagen peptide product.

10. A method for preparing a collagen cosmetic with a moisturizing effect, based on the collagen cosmetic with a moisturizing effect according to any one of claims 1 to 9, characterized in that: The steps include: S1. Prepare the following raw materials according to the mass component ratio: collagen peptide, hyaluronic acid, glycerin, vitamin C liquid, Centella asiatica extract, ceramide, ergothioneine and aloe vera juice; S2, adding collagen peptide, hyaluronic acid, glycerin, vitamin C liquid, Centella asiatica extract, ceramide, ergothioneine and aloe vera juice into a constant temperature container at a temperature of 40° C.-80° C., stirring and mixing, so that the solid raw materials are fully dissolved to obtain a mixed solution; S3, adding an emulsifier to the mixed solution for emulsification, wherein the emulsifier is set to be one of lanolin alcohol, lanolin acid and lanolin, and the emulsification temperature is between 40° C. and 80° C.; S4, using a homogenizing device to refine the emulsified mixture; S5. Cool the refined emulsion to room temperature and then package and store it.

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