Extract composition, preparation method thereof and application of extract composition in preparation of products with sun-screening and skin-moistening effects
The extract composition prepared by using camellia seeds and poria as raw materials, using acetone extraction and combined with silica gel column elution technology, solved the problem of insufficient sun protection effect in sun protection products in the prior art, and achieved significant sun protection and moisturizing effects.
Patent Information
- Application Number
- CN202411714152.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-27
- Publication Date
- 2025-03-25
AI Technical Summary
It is difficult to develop an active ingredient with significant sun protection effects for the preparation of sun protection products, especially to avoid the challenge of increasing the chance of skin cancer while improving sun protection effects and reducing skin aging.
The camellia seeds and Poria as raw materials and acetone as solvent were extracted, and the camellia seed-Poria solvent extract was further optimized by silica gel column elution technology to obtain an extract composition with significant sun protection effect.
This extract composition not only significantly improves the sun protection effect, but also has a more significant sun protection effect than the use of camellia seeds or Poria extracts alone, and further enhances its sun protection and moisturizing effect through an optimized preparation method.
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Abstract
Description
Technical Field
[0001] This invention relates to the field of biomedical technology, specifically to an extract composition, its preparation method, and its application in the preparation of products with sun protection and skin moisturizing effects. Background Technology
[0002] Excessive exposure to ultraviolet (UV) radiation damages epidermal cells; activates tyrosinase, accelerating pigment synthesis; impairs the skin's moisturizing function, leading to dryness; and damages elastic fibers in the dermis, causing fine lines. This photoaging-related skin damage may appear earlier and be more severe than natural aging. Farmers, fishermen, and other groups exposed to sunlight for extended periods often develop more wrinkles and age spots. Long-term, high-intensity UV exposure increases the risk of skin cancer. Basal cell carcinoma, squamous cell carcinoma, and melanoma are all associated with UV exposure.
[0003] Therefore, developing an active ingredient with sun protection properties to provide more raw material sources for the preparation of sun protection products has important application value for the further development of sun protection products and the prevention and treatment of sunburn. Summary of the Invention
[0004] In order to overcome at least one of the technical problems existing in the prior art, the present invention provides an extract composition. The technical solution of the present invention is as follows: A method for preparing an extract composition, characterized by comprising the following steps: taking camellia seeds and poria cocos, mixing them, and extracting them with a solvent; after extraction, concentrating and drying the extract to obtain a camellia seed-poria cocos solvent extract; and taking the camellia seed-poria cocos solvent extract to obtain the extract composition.
[0005] This invention provides an extract composition prepared by a novel method; studies have shown that the camellia seed-poria solvent extract obtained by this method using camellia seeds and poria as raw materials has sun protection properties.
[0006] Furthermore, the camellia seed-poria solvent extract obtained by using both camellia seed and poria cocos as raw materials in this invention exhibits significantly higher sun protection effects than the camellia seed-poria cocos solvent extract obtained using only camellia seed or only poria cocos as raw materials. Simultaneously using both camellia seed and poria cocos as raw materials can synergistically enhance the sun protection effect of the prepared camellia seed-poria cocos solvent extract.
[0007] Preferably, the ratio of the total weight of camellia seeds and poria cocos to the amount of solvent is 1 kg: 5~10 L.
[0008] The most preferred ratio is 1 kg to 8 L of solvent, where the total weight of camellia seeds and poria cocos is 1 kg to 8 L.
[0009] Preferably, the weight ratio of camellia seeds to poria cocos is 3~5:5~10.
[0010] The most preferred ratio is 4:7 in weight between camellia seeds and poria cocos.
[0011] Preferably, the solvent is acetone.
[0012] Further research by the inventors revealed that the choice of extraction solvent is also very important; the sun protection effect of the camellia seed-poria solvent extract obtained by acetone extraction using camellia seeds and poria as raw materials is significantly higher than that of the camellia seed-poria solvent extract obtained by water or ethanol extraction.
[0013] Preferably, the method for preparing the extract composition further includes the following steps: The camellia seed-poria solvent extract was further loaded onto a silica gel column. First, it was eluted with mixed solvent A to remove impurities; then it was eluted with mixed solvent B. The eluent obtained by eluting with mixed solvent B was collected, concentrated and dried to obtain the camellia seed-poria silica gel column elution fraction. The extract composition was obtained by taking the camellia seed-poria silica gel column elution fraction.
[0014] Preferably, the mixed solvent A is composed of cyclohexane and acetone in a volume ratio of 100:26~28; and the mixed solvent B is composed of cyclohexane and acetone in a volume ratio of 100:41~44.
[0015] Preferably, the mixed solvent A is composed of cyclohexane and acetone in a volume ratio of 100:27; and the mixed solvent B is composed of cyclohexane and acetone in a volume ratio of 100:43.
[0016] Further research by the inventors revealed that the sun protection effect of the camellia seed-poria cocos silica gel column eluted fraction obtained by further eluting the camellia seed-poria cocos solvent extract under the silica gel column elution conditions described above in this invention is significantly higher than that of the camellia seed-poria cocos solvent extract.
[0017] The inventors also discovered that the composition of the silica gel column elution solvent of the present invention is crucial; only when the camellia seed-poria cocos solvent extract is further eluted with the composition of the silica gel column elution solvent of the present invention can the sun protection effect of the camellia seed-poria cocos silica gel column elution fraction be significantly higher than that of the camellia seed-poria cocos solvent extract; however, the sun protection effect of the camellia seed-poria cocos silica gel column elution fraction obtained with other silica gel column elution solvent compositions is not significantly higher than that of the camellia seed-poria cocos solvent extract.
[0018] The present invention also provides an extract composition prepared by the above preparation method.
[0019] The present invention also provides the use of the above-mentioned extract composition in the preparation of products with sun protection function.
[0020] The present invention also provides the application of the above-mentioned extract composition in the preparation of products with sun protection and skin moisturizing effects.
[0021] Preferably, the product is a skin care product or a medicine.
[0022] Beneficial effects: This invention provides a novel method for preparing an extract composition; the extract composition is obtained by extracting camellia seeds and Poria cocos as raw materials; studies have shown that the extract composition prepared by the method of this invention has sun protection effect; therefore, it has important application value as an active ingredient in the preparation of products with sun protection effect, or in combination with other skin moisturizing active ingredients in the preparation of products with sun protection and skin moisturizing effect. Detailed Implementation
[0023] The present invention will be further described in detail below with reference to specific embodiments, but the embodiments do not limit the present invention in any way.
[0024] Example 1 Preparation of the extract composition Camellia seeds and Poria cocos were mixed and extracted for 2 hours by heating with a solvent to boiling. After extraction, the extract was concentrated and dried to obtain camellia seed-Poria cocos solvent extract. The extract composition was obtained by taking the camellia seed-Poria cocos solvent extract. The ratio of the total weight of camellia seeds and poria cocos to the amount of solvent is 1 kg: 8 L; the weight ratio of camellia seeds to poria cocos is 4:7; and the solvent is acetone.
[0025] Example 2 Preparation of the extract composition (1) Take camellia seeds and Poria cocos and mix them. Heat the mixture with a solvent to boiling point and extract for 2 hours. After extraction, concentrate and dry the extract to obtain camellia seed-Poria cocos solvent extract. The total weight of camellia seeds and Poria cocos to the amount of solvent is 1 kg: 8 L. The weight ratio of camellia seeds to Poria cocos is 4: 7. The solvent is acetone. (2) The camellia seed-poria solvent extract was further loaded onto a silica gel column. First, it was eluted with 5 column volumes of mixed solvent A to remove impurities. Then, it was eluted with 5 column volumes of mixed solvent B. The eluent eluted by mixed solvent B was collected, concentrated and dried to obtain the camellia seed-poria silica gel column elution fraction. The extract composition was obtained by taking the camellia seed-poria silica gel column elution fraction. The mixed solvent A is composed of cyclohexane and acetone in a volume ratio of 100:27; the mixed solvent B is composed of cyclohexane and acetone in a volume ratio of 100:43.
[0026] Comparative Example 1: Preparation of Extract Composition Camellia seeds were extracted by heating a solvent to boiling for 2 hours. After extraction, the extract was concentrated and dried to obtain a camellia seed solvent extract. The extract composition was obtained by taking the camellia seed solvent extract. The ratio of camellia seeds to solvent is 1 kg: 8 L; the solvent is acetone.
[0027] Comparative Example 2: Preparation of Extract Composition Poria cocos was heated to boiling in a solvent and extracted for 2 hours. After extraction, the extract was concentrated and dried to obtain a Poria cocos solvent extract. The Poria cocos solvent extract was then used to obtain the extract composition described above. The ratio of Poria cocos to solvent is 1 kg: 8 L; the solvent is acetone.
[0028] Preparation of extract composition in Comparative Example 3 Camellia seeds and Poria cocos were mixed and extracted for 2 hours by heating with a solvent to boiling. After extraction, the extract was concentrated and dried to obtain camellia seed-Poria cocos solvent extract. The extract composition was obtained by taking the camellia seed-Poria cocos solvent extract. The ratio of the total weight of camellia seeds and poria cocos to the amount of solvent is 1 kg: 8 L; the weight ratio of camellia seeds to poria cocos is 4:7; and the solvent is water.
[0029] Comparative Example 4: Preparation of Extract Composition Camellia seeds and Poria cocos were mixed and extracted for 2 hours by heating with a solvent to boiling. After extraction, the extract was concentrated and dried to obtain camellia seed-Poria cocos solvent extract. The extract composition was obtained by taking the camellia seed-Poria cocos solvent extract. The ratio of the total weight of camellia seeds and poria cocos to the amount of solvent is 1 kg: 8 L; the weight ratio of camellia seeds to poria cocos is 4:7; and the solvent is a 95% ethanol aqueous solution by volume.
[0030] Preparation of extract composition in Comparative Example 5 (1) Take camellia seeds and Poria cocos and mix them. Heat the mixture with a solvent to boiling point and extract for 2 hours. After extraction, concentrate and dry the extract to obtain camellia seed-Poria cocos solvent extract. The total weight of camellia seeds and Poria cocos to the amount of solvent is 1 kg: 8 L. The weight ratio of camellia seeds to Poria cocos is 4: 7. The solvent is acetone. (2) The camellia seed-poria solvent extract was further loaded onto a silica gel column. First, it was eluted with 5 column volumes of mixed solvent A to remove impurities. Then, it was eluted with 5 column volumes of mixed solvent B. The eluent eluted by mixed solvent B was collected, concentrated and dried to obtain the camellia seed-poria silica gel column elution fraction. The extract composition was obtained by taking the camellia seed-poria silica gel column elution fraction. The mixed solvent A is composed of cyclohexane and acetone in a volume ratio of 100:15; the mixed solvent B is composed of cyclohexane and acetone in a volume ratio of 100:30.
[0031] Preparation of extract composition in Comparative Example 6 (1) Take camellia seeds and Poria cocos and mix them. Heat the mixture with a solvent to boiling point and extract for 2 hours. After extraction, concentrate and dry the extract to obtain camellia seed-Poria cocos solvent extract. The total weight of camellia seeds and Poria cocos to the amount of solvent is 1 kg: 8 L. The weight ratio of camellia seeds to Poria cocos is 4: 7. The solvent is acetone. (2) The camellia seed-poria solvent extract was further loaded onto a silica gel column. First, it was eluted with 5 column volumes of mixed solvent A to remove impurities. Then, it was eluted with 5 column volumes of mixed solvent B. The eluent eluted by mixed solvent B was collected, concentrated and dried to obtain the camellia seed-poria silica gel column elution fraction. The extract composition was obtained by taking the camellia seed-poria silica gel column elution fraction. The mixed solvent A is composed of cyclohexane and acetone in a volume ratio of 100:40; the mixed solvent B is composed of cyclohexane and acetone in a volume ratio of 100:55.
[0032] Experiment Example 1: Cellular Sunscreen Experiment Take HaCat cells, at a ratio of 1x10 4 Add samples per well to a 96-well plate, followed by 100 μL of DMEM medium per well. After 2 days of incubation, replace each well in the experimental group with 100 μL of DMEM medium containing 1 mg / mL of the test sample, while replacing each well in the UV control and blank control groups with 100 μL of DMEM medium without the test sample. Continue incubation for another 2 days. Then, replace each well in the experimental group and the UV control group with 100 mJ / cm² DMEM medium. 2 The cells were irradiated with ultraviolet light for 20 minutes; the blank control group was not irradiated with ultraviolet light; after ultraviolet irradiation, MTT was added and the cells were treated for 4 hours; finally, the supernatant was removed, DMSO was added, and the cells were placed in the dark for 15 minutes. The absorbance at 490 nm was measured using an enzyme-linked immunosorbent assay (ELISA) reader; the cell viability was calculated based on the absorbance value; the test results are shown in Table 1.
[0033] Table 1. Sunscreen test results of the extract composition of the present invention
[0034] As can be seen from the experimental results in Table 1, the cell survival rate of the UV control group without any test sample after UV irradiation in this experiment was only 33.6%; while the cell survival rate of the extract composition prepared in Example 1 was significantly higher than that of the UV control group. This indicates that the camellia seed-poria solvent extract obtained by the present invention using camellia seed and poria as raw materials has a very significant sun protection effect.
[0035] As can be seen from the experimental results in Table 1, the cell viability of the extract composition prepared in Example 1 was significantly higher than that of the extract compositions prepared in Comparative Examples 1 and 2. This indicates that the camellia seed-poria solvent extract obtained by simultaneously using camellia seed and poria as raw materials in this invention has a significantly higher sun protection effect than the camellia seed-poria solvent extract obtained by using camellia seed or poria alone as raw materials. Simultaneous use of camellia seed and poria can synergistically enhance the sun protection effect of the prepared camellia seed-poria solvent extract.
[0036] As can be seen from the experimental results in Table 1, the cell survival rate of the extract composition prepared in Example 1 is significantly higher than that of the extract compositions prepared in Comparative Examples 3 and 4. This indicates that the selection of the extraction solvent in this invention is also very important. The camellia seed-poria solvent extract obtained by acetone extraction using camellia seeds and poria as raw materials has a significantly higher sun protection effect than the camellia seed-poria solvent extract obtained by water or ethanol extraction.
[0037] As can be seen from the experimental results in Table 1, the cell survival rate of the extract composition prepared in Example 2 is significantly higher than that of the extract composition prepared in Example 1. This indicates that the sun protection effect of the camellia seed-poria cocos silica column eluted fraction obtained by further eluting the camellia seed-poria cocos solvent extract under the silica column elution conditions described above in this invention is significantly higher than that of the camellia seed-poria cocos solvent extract.
[0038] As can be seen from the experimental results in Table 1, the cell viability of the extract compositions prepared in Comparative Examples 5 and 6 was not significantly improved compared with that of the extract composition prepared in Example 1. This indicates that the composition of the silica gel column elution solvent of the present invention is crucial. Only when the Camellia seed-Poria cocos solvent extract is further eluted with the composition of the silica gel column elution solvent of the present invention can the sun protection effect of the Camellia seed-Poria cocos silica gel column elution fraction be significantly higher than that of the Camellia seed-Poria cocos solvent extract. However, the sun protection effect of the Camellia seed-Poria cocos silica gel column elution fraction obtained with other silica gel column elution solvent compositions is not significantly higher than that of the Camellia seed-Poria cocos solvent extract.
Claims
1. A method for preparing an extract composition, characterized in that: The method comprises the following steps: mixing camellia seeds and poria cocos and extracting with a solvent; after the extraction, concentrating and drying the extract to obtain a camellia seed-poria cocos solvent extract; and taking the camellia seed-poria cocos solvent extract to obtain the extract composition.
2. The method for preparing the extract composition according to claim 1, characterized in that: The ratio of the total weight of camellia seeds and Poria cocos to the solvent is 1kg:5~10L.
3. The method for preparing the extract composition according to claim 1, characterized in that: The weight ratio of camellia seeds to Poria cocos is 3~5:5~10.
4. The method for preparing the extract composition according to claim 1, characterized in that: The solvent is acetone.
5. The method for preparing the extract composition according to claim 1, characterized in that: The preparation method of the extract composition further comprises the following steps: The camellia seed-Poria cocos solvent extract is further applied to a silica gel column, and first eluted with a mixed solvent A to remove impurities; then eluted with a mixed solvent B, and the eluate eluted with the mixed solvent B is collected, concentrated and dried to obtain the camellia seed-Poria cocos silica gel column elution portion; the camellia seed-Poria cocos silica gel column elution portion is taken to obtain the extract composition.
6. The method for preparing the extract composition according to claim 5, characterized in that: The mixed solvent A is composed of cyclohexane and acetone in a volume ratio of 100:26-28; the mixed solvent B is composed of cyclohexane and acetone in a volume ratio of 100:41-44.
7. The method for preparing the extract composition according to claim 6, characterized in that: The mixed solvent A is composed of cyclohexane and acetone in a volume ratio of 100:27; the mixed solvent B is composed of cyclohexane and acetone in a volume ratio of 100:
43.
8. The extract composition obtained by the preparation method according to any one of claims 1 to 7.
9. Use of the extract composition according to claim 8 in preparing a product having a sunscreen effect.
10. Use of the extract composition according to claim 8 in preparing a product having sunscreen and skin moisturizing effects.