Lactobacillus anhydrous dental tablet containing viable bacteria and preparation method thereof

Abstract

The invention relates to a viable bacterium-containing lactobacillus anhydrous dental tablet and a preparation method thereof, and belongs to the technical field of oral care. The lactobacillus anhydrous dental tablet containing viable bacteria comprises the following raw materials in percentage by mass: 0.01%-3% of active lactobacillus coated with a water-soluble zein film, 0.2%-15% of a nutritional additive, 5%-22% of an organic acid disintegrating agent, 10%-25% of an inorganic alkaline disintegrating agent, 6%-40% of an adhesive, 1.1%-38% of a lubricant, 0.6%-7% of a friction agent, 0.01%-1.5% of a foaming agent, 1.1%-20% of a sweetening agent, 0.01%-2% of essence and 0.05%-0.1% of sustained-release nicotinamide. The active lactobacillus is instantly released during tooth brushing to play an antibacterial role, and the polydopamine nanoparticles can play a role in slowly releasing nicotinamide, so that the nicotinamide is slowly released in an oral cavity to continuously resist bacteria.

Description

Technical Field

[0001] The invention belongs to the technical field of oral care, and particularly relates to an anhydrous lactobacillus tooth tablet containing live bacteria and a preparation method thereof. Background Art

[0002] In recent years, the concept of oral products containing lactobacillus has become more and more abundant, and toothpaste has become the main carrier of lactobacillus, but toothpaste has a high water content, and live bacteria can survive and multiply in toothpaste, resulting in unstable toothpaste paste and quality problems, which is not conducive to the stable existence of probiotics; and many conceptual products with the name of "lactobacillus" have appeared on the market, and their lactobacillus are all dead bacteria that have been inactivated, which can not compete with harmful oral bacteria to exclude and maintain the real meaning of oral flora. The invention patent with application publication number CN117100675A discloses a lactobacillus anhydrous tooth tablet containing live bacteria and its preparation method and application, the lactobacillus anhydrous tooth tablet containing live bacteria ensures that lactobacillus is dormant in a suitable environment through an anhydrous system, and the activity of activated lactobacillus after water is used to regulate the microecological balance of oral flora, so as to achieve the effect of removing bad breath and long-lasting fresh breath, but the active lactobacillus storage environment is low temperature, and the active lactobacillus is easily inactivated when stored at room temperature. Summary of the invention

[0003] The first object of the present invention is to provide a lactobacillus anhydrous tooth tablet containing live bacteria to solve the technical problem that active lactobacillus in the anhydrous tooth tablet is easily inactivated.

[0004] The second object of the present invention is to provide a method for preparing anhydrous lactobacillus dental tablets containing live bacteria.

[0005] In order to achieve the above purpose, the technical solution adopted by the present invention is: A lactobacillus anhydrous tooth tablet containing live bacteria comprises the following raw materials calculated by mass percentage: 0.01% to 3% of active lactobacillus coated with a water-soluble zein film, 0.2% to 15% of nutrient additives, 5% to 22% of organic acid disintegrants, 10% to 25% of inorganic alkaline disintegrants, 6% to 40% of adhesives, 1.1% to 38% of lubricants, 0.6% to 7% of friction agents, 0.01% to 1.5% of foaming agents, 1.1% to 20% of sweeteners, 0.01% to 2% of flavors, and 0.05% to 0.1% of sustained-release nicotinamide.

[0006] Furthermore, the preparation method of the active lactobacillus coated with the water-soluble zein film is: S1: dissolving water-soluble zein in water, slowly adding a plasticizer, stirring and heating to obtain a water-soluble zein film-forming solution; S2: drying the active lactobacillus bacterial sludge to obtain bacterial powder, spraying the water-soluble zein film-forming liquid onto the bacterial powder, and drying to obtain the obtained product.

[0007] Furthermore, the plasticizer is polyethylene glycol 200; the mass ratio of the water-soluble zein to the plasticizer is 1:0.3-0.5; and the concentration of the water-soluble zein in the water-soluble zein film-forming solution is 0.1-0.2 g / mL.

[0008] Furthermore, the heating temperature in S1 is 40-70° C., and the stirring time is 20-30 min.

[0009] Furthermore, the preparation method of the sustained-release nicotinamide is as follows: polydopamine nanoparticles are dispersed in ethanol to obtain a dispersion, and the dispersion is sprayed onto nicotinamide powder and dried to obtain the sustained-release nicotinamide.

[0010] Furthermore, the concentration of the polydopamine ethanol solution is 5 to 8 mg / mL.

[0011] Furthermore, the active lactobacillus is one or more of Lactobacillus paracasei ET-66 and Lactobacillus salivarius AP-32; the nutritional additive is one or more of prebiotics, oligofructose, and glucose; the organic acid disintegrant is one or more of citric acid, malic acid, tartaric acid, and fumaric acid; and the inorganic alkaline disintegrant is sodium bicarbonate.

[0012] Furthermore, the adhesive is one or more of microcrystalline cellulose, lactose, polyvinyl pyrrolidone, and sodium carboxymethyl cellulose; the lubricant is one or more of polyethylene glycol 4000, polyethylene glycol 6000, magnesium stearate, and maltodextrin; and the friction agent is one or more of stone powder, silicon dioxide, calcium hydrogen phosphate, and aluminum hydroxide.

[0013] Furthermore, the foaming agent is one or more of sodium lauroyl sarcosinate, sodium lauryl sulfate, betaine, and sodium methyl cocoyl taurate; the sweetener is one or more of sorbitol, erythritol, xylitol, sucralose, and mannitol; and the flavor is one or more of fruit flavor, flower flavor, tea flavor, mint flavor, and candy flavor.

[0014] A method for preparing anhydrous lactobacillus dental tablets containing live bacteria comprises the following steps: S1: Preparation of acid granules: an organic acid disintegrant and a lubricant are mixed and sieved, and then mixed in equal and increasing amounts to obtain a mixture 1; the mixture 1 is mixed with a polyvinyl pyrrolidone solution and sieved to obtain granules, and then dried and sieved to obtain granules; S2: Preparation of alkaline granules: an inorganic alkaline disintegrant, abrasive, foaming agent, flavor, lubricant, sweetener and nutritional additive are mixed and sieved, and then mixed in equal and increasing amounts to obtain a mixture 2, and the mixture 2 is mixed with a polyvinyl pyrrolidone solution and sieved to obtain granules, and then dried and sieved to obtain whole granules; S3: Mixing and tableting: The acid particles in S1 and the base particles in S2 are mixed, and then active lactobacillus coated with a water-soluble zein film, sustained-release nicotinamide and the remaining adhesives, lubricants, sweeteners, and nutritional additives are added and mixed, and tablets are formed under a pressure of 2 to 4 MPa.

[0015] Beneficial effects of the present invention: The present invention wraps a layer of water-soluble zein film on the outside of active lactobacilli, and the water-soluble zein film can improve the survival rate of active lactobacilli at room temperature and high temperature, thereby avoiding the problem of inactivation of active lactobacilli when the lactobacilli anhydrous tooth piece containing live bacteria of the present invention is stored at room temperature. The water-soluble zein of the present invention will dissolve after encountering water, thereby releasing a large amount of active lactobacilli, thereby inhibiting the activity of oral pathogens, and then achieving the effect of eliminating bad breath and long-term fresh breath. The polydopamine nanoparticles can be adsorbed in the oral cavity, and the polydopamine nanoparticles can play a role of sustained release of the nicotinamide coated thereon, thereby allowing the nicotinamide in the oral cavity to continue to play the effect of resisting Porphyromonas gingivalis.

[0016] The active lactobacillus of the present invention is instantly released when brushing teeth to play an antibacterial role, and the polydopamine nanoparticles can play a role in sustained release of nicotinamide, thereby causing the nicotinamide to be slowly released in the oral cavity to continuously play an antibacterial role.

[0017] The present invention adds sodium bicarbonate, which will generate a large amount of carbon dioxide gas when it meets water. The carbon dioxide gas can drive the released active lactobacilli to disperse into the oral cavity, thereby avoiding uneven distribution of the active lactobacilli. DETAILED DESCRIPTION

[0018] The present invention will be further described below in conjunction with the embodiments of the present invention.

[0019] The preparation method of water-soluble zein is as follows: 6 g of polyethylene glycol 6000 is dissolved in 40 mL of acetone, stirred at room temperature, 1.7 mL of Jones reagent is added, reacted for 10 h, 0.6 g of activated carbon is added and reacted for 3 h, filtered and decarbonized, acetone is removed, and polyethylene glycol carboxylic acid derivatives are obtained. 3 g of polyethylene glycol carboxylic acid derivatives are dissolved in 20 mL of thionyl chloride, stirred, heated at 80 °C and refluxed for 8 h, thionyl chloride is removed to obtain polyethylene glycol chloride derivatives, 1 g of zein is dissolved in 10 mL of dimethylformamide, then polyethylene glycol chloride derivatives are added, heated at 60 °C and reacted for 10 h under stirring, dimethylformamide is removed, and the product is obtained after drying. Example 1

[0020] The lactobacillus anhydrous tooth tablets containing live bacteria of Example 1 include the following raw materials: 3 g of active lactobacillus coated with a water-soluble zein film, 8.45 g of nutritional additives, 13 g of an organic acid disintegrant, 20 g of an inorganic alkaline disintegrant; 29.2 g of a binder; 11.5 g of a lubricant; 3 g of a friction agent; 0.5 g of a foaming agent; 10.5 g of a sweetener; 0.8 g of a mint flavor, and 0.05 g of sustained-release niacinamide.

[0021] Active lactobacilli include 1.5 g of Lactobacillus salivarius AP-32 and 1.5 g of Lactobacillus paracasei ET-66; nutritional additives include 0.45 g of oligofructose and 8 g of glucose; organic acid disintegrants include 8 g of citric acid and 5 g of malic acid; the inorganic alkaline disintegrant is sodium bicarbonate; the binder includes 15 g of microcrystalline cellulose and 14.2 g of lactose; the lubricant includes 5 g of polyethylene glycol 6000 and 6.5 g of maltodextrin; the abrasive includes 0.5 g of stone powder and 2.5 g of silicon dioxide; the foaming agent is sodium lauroyl sarcosinate; the sweetener includes 10 g of sorbitol and 0.5 g of xylitol.

[0022] The preparation method of the sustained-release nicotinamide is as follows: polydopamine nanoparticles are dispersed in ethanol to obtain a dispersion, the dispersion is sprayed onto nicotinamide powder, and the dispersion is dried to obtain the product. The concentration of the polydopamine nanoparticles in the dispersion is 5 mg / mL.

[0023] The preparation method of active lactobacillus coated with water-soluble zein film is as follows: S1: Dissolve 10 g of water-soluble zein in water, then slowly drop 3 g of plasticizer, stir for 30 min, heat in a water bath for 50 min, and stir for 30 min to obtain a water-soluble zein film-forming solution; S2: Dry the active lactobacillus bacterial sludge to obtain bacterial powder, spray the water-soluble zein film-forming liquid onto the outside of the bacterial powder, and dry it to obtain the powder.

[0024] The plasticizer is polyethylene glycol 200; the concentration of water-soluble zein in the water-soluble zein film-forming solution is 0.1 g / mL.

[0025] The preparation method of the lactobacillus anhydrous dental tablets containing live bacteria of Example 1 comprises the following steps: S1: Preparation of acid granules: Mix the organic acid disintegrant and 3 g maltodextrin in the formula amount, pass through a 40-mesh sieve, and then mix them in equal and increasing amounts to obtain a mixture 1; mix the mixture 1 with a polyvinyl pyrrolidone solution with a mass fraction of 5%, pass through a 20-mesh sieve to granulate, dry at 50°C for 3 h, and then pass through a 20-mesh sieve to obtain the obtained granules; the solvent of the polyvinyl pyrrolidone solution is 75% by volume ethanol; S2: Preparation of alkaline granules: Inorganic alkaline disintegrant, abrasive, foaming agent, flavor, lactose, 3.5 g maltodextrin, sorbitol and glucose were mixed and passed through a 40-mesh sieve, and then mixed in equal and increasing amounts to obtain mixed material 2, mixed with 5% polyvinyl pyrrolidone solution by mass, passed through a 20-mesh sieve for granulation, dried at 50°C for 3h, and then passed through a 20-mesh sieve for granulation, to obtain; the solvent of the polyvinyl pyrrolidone solution is 75% ethanol by volume; S3: Mixing and tableting: The acid particles in S1 and the base particles in S2 are mixed, and then active lactobacillus coated with a water-soluble zein film, sustained-release nicotinamide, microcrystalline cellulose, polyethylene glycol 600, xylitol, and oligofructose are added and mixed, and tablets are formed under a pressure of 2 MPa. Example 2

[0026] The lactobacillus anhydrous tooth tablets containing live bacteria of Example 2 include the following raw materials: 1 kg of active lactobacillus coated with a water-soluble zein film, 6.35 kg of nutritional additives, 21 kg of organic acid disintegrants, 25 kg of inorganic alkaline disintegrants; 23 kg of adhesives; 17 kg of lubricants; 1.5 kg of abrasives; 0.03 kg of foaming agents; 5 kg of sweeteners; 0.05 kg of fruit flavors, and 0.07 kg of sustained-release niacinamide.

[0027] Active lactobacilli include 0.5 kg of Lactobacillus salivarius AP-32 and 0.5 kg of Lactobacillus paracasei ET-66; nutritional additives include 3.35 kg of oligofructose and 3 kg of prebiotics; organic acid disintegrants include 11 kg of tartaric acid and 10 kg of fumaric acid; the inorganic alkaline disintegrant is sodium bicarbonate; the binder includes 10 kg of microcrystalline cellulose and 13 kg of sodium carboxymethyl cellulose; the lubricant includes 8 kg of polyethylene glycol 6000 and 9 kg of magnesium stearate; the abrasive includes 0.5 kg of calcium hydrogen phosphate and 1 kg of aluminum hydroxide; the foaming agent is betaine; the sweetener includes 2.5 kg of xylitol and 2.5 kg of sucralose.

[0028] The preparation method of the sustained-release nicotinamide is as follows: polydopamine nanoparticles are dispersed in ethanol to obtain a dispersion, the dispersion is sprayed onto nicotinamide powder, and the dispersion is dried to obtain the product. The concentration of the polydopamine nanoparticles in the dispersion is 7 mg / mL.

[0029] The preparation method of active lactobacillus coated with water-soluble zein film is as follows: S1: Dissolve 10 g of water-soluble zein in water, then slowly drop 5 g of plasticizer, stir for 20 min, heat in a water bath for 70 min, and stir for 30 min to obtain a water-soluble zein film-forming solution; S2: Dry the active lactobacillus bacterial sludge to obtain bacterial powder, spray the water-soluble zein film-forming liquid onto the outside of the bacterial powder, and dry it to obtain the powder.

[0030] The plasticizer is polyethylene glycol 200; the concentration of water-soluble zein in the water-soluble zein film-forming solution is 0.15 g / mL.

[0031] The preparation method of the lactobacillus anhydrous dental tablets containing live bacteria of Example 2 comprises the following steps: S1: Preparation of acid granules: Mix the organic acid disintegrant in the formula amount and 4.5 kg of magnesium stearate, then pass through a 40-mesh sieve, and then mix them in equal and increasing amounts to obtain a mixture 1; mix the mixture 1 with a polyvinyl pyrrolidone solution with a mass fraction of 5%, pass through a 20-mesh sieve to granulate, dry at 50°C for 3 h, and then pass through a 20-mesh sieve to obtain the obtained granules; the solvent of the polyvinyl pyrrolidone solution is 75% by volume ethanol; S2: Preparation of alkaline granules: Inorganic alkaline disintegrant, abrasive, foaming agent, flavor, sodium carboxymethyl cellulose, 4.5 kg magnesium stearate, xylitol and prebiotics are mixed and passed through a 40-mesh sieve, and then mixed in equal and increasing amounts to obtain mixed material 2, mixed with 5% polyvinyl pyrrolidone solution by mass, and then passed through a 20-mesh sieve for granulation, dried at 50°C for 3 h, and then passed through a 20-mesh sieve for granulation, to obtain; the solvent of the polyvinyl pyrrolidone solution is 75% ethanol by volume; S3: Mixed tableting: The acid particles in S1 and the base particles in S2 are mixed, and then active lactobacillus coated with a water-soluble zein film, sustained-release nicotinamide, microcrystalline cellulose, polyethylene glycol 6000, sucralose, and oligofructose are added and mixed, and tablets are formed under a pressure of 3 MPa. Example 3

[0032] The lactobacillus anhydrous tooth tablets containing live bacteria of Example 3 include the following raw materials: 2 kg of active lactobacillus coated with a water-soluble zein film, 13 kg of nutritional additives, 12.4 kg of an organic acid disintegrant, 10 kg of an inorganic alkaline disintegrant; 20 kg of a binder; 20 kg of a lubricant; 6 kg of a friction agent; 1.5 kg of a foaming agent; 13 kg of a sweetener; 2 kg of fruit flavor, and 0.1 kg of sustained-release niacinamide.

[0033] Active lactobacilli include 1 kg of Lactobacillus salivarius AP-32 and 1 kg of Lactobacillus paracasei ET-66; nutritional additives include 6.5 kg of glucose and 6.5 kg of prebiotics; organic acid disintegrants include 11 kg of tartaric acid and 11.4 kg of malic acid; the inorganic alkaline disintegrant is sodium bicarbonate; the binder includes 10 kg of microcrystalline cellulose and 10 kg of lactose; the lubricant includes 10 kg of polyethylene glycol 4000 and 10 kg of maltodextrin; the friction agent is calcium hydrogen phosphate; the foaming agent includes 0.75 kg of sodium lauryl sulfate and 0.75 kg of sodium methyl cocoyl taurate; the sweetener includes 8 kg of xylitol and 5 kg of mannitol.

[0034] The preparation method of the sustained-release nicotinamide is as follows: polydopamine nanoparticles are dispersed in ethanol to obtain a dispersion, the dispersion is sprayed onto nicotinamide powder, and the dispersion is dried to obtain the product. The concentration of the polydopamine nanoparticles in the dispersion is 8 mg / mL.

[0035] The preparation method of active lactobacillus coated with water-soluble zein film is as follows: S1: Dissolve 10 g of water-soluble zein in water, then slowly drop 4 g of plasticizer, stir for 30 min, heat in a water bath for 40 min, and stir for 30 min to obtain a water-soluble zein film-forming solution; S2: Dry the active lactobacillus bacterial sludge to obtain bacterial powder, spray the water-soluble zein film-forming liquid onto the outside of the bacterial powder, and dry it to obtain the powder.

[0036] The plasticizer is polyethylene glycol 200; the concentration of water-soluble zein in the water-soluble zein film-forming solution is 0.2 g / mL.

[0037] The preparation method of the lactobacillus anhydrous dental tablets containing live bacteria of Example 3 comprises the following steps: S1: Preparation of acid granules: Mix the organic acid disintegrant in the formula amount and 5 kg of maltodextrin, then pass through a 40-mesh sieve, and then mix them in equal and increasing amounts to obtain a mixture 1; mix the mixture 1 with a polyvinyl pyrrolidone solution with a mass fraction of 5%, pass through a 20-mesh sieve to granulate, dry at 50°C for 3 h, and then pass through a 20-mesh sieve to obtain the obtained granules; the solvent of the polyvinyl pyrrolidone solution is 75% by volume ethanol; S2: Preparation of alkaline granules: Inorganic alkaline disintegrant, abrasive, foaming agent, flavor, lactose, 5 kg maltodextrin, mannitol and prebiotics are mixed and passed through a 40-mesh sieve, and then mixed in equal and increasing amounts to obtain mixed material 2, mixed with 5% polyvinyl pyrrolidone solution by mass, and then passed through a 20-mesh sieve for granulation, dried at 50°C for 3 h, and then passed through a 20-mesh sieve for granulation, to obtain; the solvent of the polyvinyl pyrrolidone solution is 75% ethanol by volume; S3: Mixing and tableting: The acid particles in S1 and the base particles in S2 are mixed, and then active lactobacillus coated with a water-soluble zein film, sustained-release nicotinamide, microcrystalline cellulose, polyethylene glycol 4000, xylitol, and glucose are added and mixed, and tablets are formed under a pressure of 4 MPa.

[0038] Comparative Example 1 The lactobacillus anhydrous tooth tablets containing live bacteria of Comparative Example 1 include the following raw materials: 3 g of active lactobacillus coated with a water-soluble zein film, 8.5 g of nutritional additives, 13 g of an organic acid disintegrant, 20 g of an inorganic alkaline disintegrant; 29.2 g of a binder; 11.5 g of a lubricant; 3 g of a friction agent; 0.5 g of a foaming agent; 10.5 g of a sweetener; and 0.8 g of a mint flavor.

[0039] The preparation method of the lactobacillus anhydrous tooth tablet containing live bacteria in Comparative Example 1 is the same as that in Example 1, and the preparation method of the active lactobacillus coated with a water-soluble zein film in Comparative Example 1 is the same as that in Example 1.

[0040] Active lactobacilli include 1.5 g of Lactobacillus salivarius AP-32 and 1.5 g of Lactobacillus paracasei ET-66; nutritional additives include 0.5 g of oligofructose and 8 g of glucose; organic acid disintegrants include 8 g of citric acid and 5 g of malic acid; the inorganic alkaline disintegrant is sodium bicarbonate; the binder includes 15 g of microcrystalline cellulose and 14.2 g of lactose; the lubricant includes 5 g of polyethylene glycol 6000 and 6.5 g of maltodextrin; the abrasive includes 0.5 g of stone powder and 2.5 g of silicon dioxide; the foaming agent is sodium lauroyl sarcosinate; the sweetener includes 10 g of sorbitol and 0.5 g of xylitol.

[0041] Test Example 1 The lactobacillus anhydrous dental tablets containing live bacteria of Example 1 were tested for the total number of lactobacilli with reference to the test method of GB 4789.35-2016. The test result was 6.5×10 6 CFU / g.

[0042] Test Example 2 1. The experimental materials are the lactobacillus anhydrous tooth tablets containing live bacteria in Example 1 and Comparative Example 1.

[0043] 2. Experimental method: 40 patients aged 25 to 50 years old who had bad breath or bad odor were randomly divided into 2 groups, 20 in each group. Each group used the corresponding test samples for oral care, and used the test samples for oral care or brushing teeth every morning and evening for 1 month to observe the effect on the symptoms of bad breath or bad odor.

[0044] 3. Evaluation criteria: The evaluation of treatment effect is divided into 4 categories: markedly effective, effective, slightly effective and ineffective. Markedly effective means that after the patient uses the test sample for 1 month, the symptoms of bad breath and bad odor in the mouth disappear, and the teeth are firm; effective means that after the patient uses the test sample for 1 month, the symptoms of bad breath and bad odor in the mouth are greatly improved; slightly effective means that after the patient uses the test sample for 1 month, the symptoms of bad breath and bad odor in the mouth are alleviated but still exist occasionally; ineffective means that after the patient uses the test sample for 1 month, the symptoms of bad breath and bad odor in the mouth are not improved.

[0045] 4. Effect evaluation: The evaluation results are shown in Table 1.

[0046] Table 1 Breath freshening results of the Lactobacillus anhydrous tooth tablets containing live bacteria in Example 1 and Comparative Example 1

[0047] As can be seen from Table 1, the lactobacillus anhydrous tooth tablets containing live bacteria in Example 1 and Comparative Example 1 have a good effect on alleviating symptoms such as halitosis and bad breath, and the effective rate is more than 90%.

[0048] Test Example 3 After 4 h of toothbrushing, the number of Porphyromonas gingivalis in the patient's oral cavity was detected by fluorescence quantitative PCR. The results are shown in Table 2. Each group of data was measured three times.

[0049] Table 2 Effect of the Lactobacillus anhydrous dental tablets containing live bacteria in Example 1 and Comparative Example 1 against Porphyromonas gingivalis

[0050] As can be seen from Table 2, after brushing teeth, the Lactobacillus anhydrous tooth tablet containing live bacteria of Example 1 still continues to exert its anti-Porphyromonas gingivalis effect due to the effect of sustained-release nicotinamide, but most of the active lactobacilli in the Lactobacillus anhydrous tooth tablet containing live bacteria of Comparative Example 1 are washed away with clean water after brushing teeth, making it difficult to continue to exert its antibacterial effect.

Claims

1. A lactobacillus anhydrous dental tablet containing live bacteria, characterized in that: The following raw materials are included by mass percentage: 0.01% to 3% of active lactobacillus coated with a water-soluble zein film, 0.2% to 15% of nutritional additives, 5% to 22% of organic acid disintegrants, 10% to 25% of inorganic alkaline disintegrants, 6% to 40% of adhesives, 1.1% to 38% of lubricants, 0.6% to 7% of friction agents, 0.01% to 1.5% of foaming agents, 1.1% to 20% of sweeteners, 0.01% to 2% of flavors, and 0.05% to 0.1% of sustained-release nicotinamide.

2. The lactobacillus anhydrous dental tablet containing live bacteria according to claim 1, characterized in that: The preparation method of the active lactobacillus coated with the water-soluble zein film is as follows: S1: dissolving water-soluble zein in water, slowly adding a plasticizer, stirring and heating to obtain a water-soluble zein film-forming solution; S2: drying the active lactobacillus bacterial sludge to obtain bacterial powder, spraying the water-soluble zein film-forming liquid onto the bacterial powder, and drying to obtain the obtained product.

3. The lactobacillus anhydrous dental tablet containing live bacteria according to claim 2, characterized in that: The plasticizer is polyethylene glycol 200; the mass ratio of the water-soluble zein to the plasticizer is 1:0.3-0.5; and the concentration of the water-soluble zein in the water-soluble zein film-forming liquid is 0.1-0.2 g / mL.

4. The lactobacillus anhydrous dental tablet containing live bacteria according to claim 2, characterized in that: The heating temperature in S1 is 40-70° C., and the stirring time is 20-30 min.

5. The lactobacillus anhydrous tooth tablet containing live bacteria according to claim 1, characterized in that: The preparation method of the sustained-release nicotinamide comprises the following steps: dispersing polydopamine nanoparticles in ethanol to obtain a dispersion, spraying the dispersion onto nicotinamide powder, and drying to obtain the sustained-release nicotinamide.

6. The lactobacillus anhydrous dental tablet containing live bacteria according to claim 5, characterized in that: The concentration of the dispersion is 5-8 mg / mL.

7. The lactobacillus anhydrous dental tablet containing live bacteria according to claim 1, characterized in that: The active lactobacillus is one or more of Lactobacillus paracasei ET-66 and Lactobacillus salivarius AP-32; the nutritional additive is one or more of prebiotics, oligofructose and glucose; the organic acid disintegrant is one or more of citric acid, malic acid, tartaric acid and fumaric acid; and the inorganic alkaline disintegrant is sodium bicarbonate.

8. The lactobacillus anhydrous tooth tablet containing live bacteria according to claim 1, characterized in that: The adhesive is one or more of microcrystalline cellulose, lactose, polyvinyl pyrrolidone, and sodium carboxymethyl cellulose; the lubricant is one or more of polyethylene glycol 4000, polyethylene glycol 6000, magnesium stearate, and maltodextrin; and the friction agent is one or more of stone powder, silicon dioxide, calcium hydrogen phosphate, and aluminum hydroxide.

9. The lactobacillus anhydrous dental tablet containing live bacteria according to claim 1, characterized in that: The foaming agent is one or more of sodium lauroyl sarcosinate, sodium lauryl sulfate, betaine, and sodium methyl cocoyl taurate; the sweetener is one or more of sorbitol, erythritol, xylitol, sucralose, and mannitol; and the flavor is one or more of fruit flavor, flower flavor, tea flavor, mint flavor, and candy flavor.

10. A method for preparing anhydrous lactobacillus dental tablets containing live bacteria as claimed in claim 1, characterized in that: The following steps are involved: S1: Preparation of acid granules: an organic acid disintegrant and a lubricant are mixed and sieved, and then mixed in equal and increasing amounts to obtain a mixture 1; the mixture 1 is mixed with a polyvinyl pyrrolidone solution and sieved to obtain granules, and then dried and sieved to obtain granules; S2: Preparation of alkaline granules: an inorganic alkaline disintegrant, abrasive, foaming agent, flavor, lubricant, sweetener and nutritional additive are mixed and sieved, and then mixed in equal and increasing amounts to obtain a mixture 2, and the mixture 2 is mixed with a polyvinyl pyrrolidone solution and sieved to obtain granules, and then dried and sieved to obtain whole granules; S3: Mixing and tableting: The acid particles in S1 and the base particles in S2 are mixed, and then active lactobacillus coated with a water-soluble zein film, sustained-release nicotinamide and the remaining adhesives, lubricants, sweeteners, and nutritional additives are added and mixed, and tablets are formed under a pressure of 2 to 4 MPa.

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