Composite camellia oil gel for treating burns and scalds as well as preparation method and application of composite camellia oil gel

Abstract

The invention discloses composite camellia oil gel for treating burns and scalds as well as a preparation method and application of the composite camellia oil gel, glycerol monolaurate is used as a gel enhancer and a bacteriostatic agent, xanthan gum and sodium alginate are used as oil gel, and the composite camellia oil gel is prepared; comprising the following steps: adding glycerol monolaurate into camellia-seed oil, and continuously stirring until the glycerol monolaurate is completely dissolved; filtering the obtained mixture by using a membrane with the molecular weight of 5-20 kDa, and freezing the obtained filtrate overnight; and adding the polysaccharide gel solution into a mixture of glycerol monolaurate and camellia oil, and carrying out homogeneous mixing, shearing and freeze-drying treatment to obtain the camellia oil gel. According to the composite camellia oil gel for treating burns and scalds as well as the preparation method and the application of the composite camellia oil gel, the microenvironment of a wound is adjusted, inflammation in the body is relieved, collagen deposition is increased, angiogenesis is promoted, wound healing is promoted, and scars are reduced. The method provided by the invention is mild and simple in reaction condition and easy for industrial production.

Description

Technical Field

[0001] The invention relates to the technical field of burn and scald plasters, in particular to a composite camellia oil gel for treating burns and scalds, and a preparation method and application thereof. Background Art

[0002] Burns and scalds refer to diseases caused by various physical or chemical factors, including hot liquids, steam, flames, etc., which cause tissue damage. Effective wound treatment and timely medication can quickly reduce sustained damage and accelerate recovery, but improper medication will not only delay recovery but also cause scars. Wound healing mainly involves bacterial infection and inflammation, oxidative stress, cell proliferation and tissue remodeling stages. It is a process of spontaneously and orderly restoring the continuity and integrity of the skin and maintaining a relatively stable internal environment. Commonly used drugs include antibiotics and traditional Chinese medicines. Long-term use will increase dependence and cannot regulate the metabolic balance of collagen in the wound.

[0003] Camellia oleifera belongs to the genus Camellia of the family Theaceae. It is a unique edible oil tree species in my country. It is known as the world's four major woody oil plants together with olive, oil palm and coconut. Camellia oil has a protective and therapeutic effect on the skin because it is rich in multiple active ingredients such as tea polyphenols, tocopherols and phytosterols. At present, CN1616052 and CN115006472A patents disclose a single camellia oil burn ointment, but it only prevents wound infection by containing active ingredients and isolating air, resulting in a single effect of promoting wound healing. Therefore, in order to improve the limitations of camellia oil in wound healing, a composite camellia oil gel with adjustable morphology, high oil content and high efficacy is prepared to improve the wound healing effect, which has broad application prospects in the prevention and treatment of scalded skin diseases and medical beauty fields. Summary of the invention

[0004] The purpose of the present invention is to provide a composite camellia oil gel for treating burns and scalds and a preparation method and application thereof, so as to solve the above problems.

[0005] To achieve the above purpose, the present invention provides a method for preparing a composite camellia oil gel for treating burns and scalds. The composite camellia oil gel is prepared by taking camellia oil as a raw material, taking lauric acid monoglyceride as a gel strengthener and antibacterial agent, and taking xanthan gum and sodium alginate as oil gelling agents. In different phase systems, the mass fraction of the camellia oil is 81wt%, the concentration of the lauric acid monoglyceride is 7.0wt%, the concentration of the sodium alginate solution is 4.8%, and the concentration of the xanthan gum solution is 7.2%.

[0006] Wherein, the unsaturated fatty acid content in the camellia seed oil is greater than 85wt%.

[0007] Preferably, in the above-mentioned composite camellia oil gel for treating burns and scalds and its preparation method, the preparation method comprises the following steps:

[0008] The monolaurin is added to the camellia oil and stirred continuously until it is completely dissolved; the stirring rate is 3000-5000 rpm; the reaction condition is 80° C. for 0.5 h;

[0009] The obtained mixture was filtered through a 5-20 kDa membrane, and the obtained filtrate was frozen overnight; the freezing time was 24 hours, and the freezing temperature was 4°C;

[0010] preparing a polysaccharide xanthan gum solution and a sodium alginate solution, and mixing the polysaccharide xanthan gum solution and the sodium alginate solution to obtain a xanthan gum-sodium alginate polysaccharide gel;

[0011] The polysaccharide gel solution is added into the mixture of lauric acid monoglyceride and camellia oil, and the composite camellia oil gel is obtained through homogenous mixing, shearing and freeze drying.

[0012] Preferably, in the above-mentioned composite camellia oil gel for treating burns and scalds and its preparation method, the xanthan gum is in liquid form to prepare a polysaccharide xanthan gum solution, and the preparation method of the polysaccharide xanthan gum solution is: dissolving the polysaccharide xanthan gum in deionized water and stirring magnetically to fully hydrate to obtain a xanthan gum solution of a certain concentration, cooling to room temperature and then refrigerating at 4°C.

[0013] Preferably, in the composite camellia oil gel for treating burns and scalds and its preparation method, the sodium alginate is in liquid form to prepare a sodium alginate solution, and the preparation method of the sodium alginate solution is: dissolving sodium alginate in deionized water, heating in a water bath until completely dissolved to obtain a sodium alginate solution of a certain concentration, cooling to room temperature and then refrigerating at 4°C;

[0014] Preferably, in the above-mentioned composite camellia oil gel for treating burns and scalds and its preparation method, the xanthan gum-sodium alginate polysaccharide gel is constructed by: fully mixing the polysaccharide xanthan gum and sodium alginate solution, and adjusting the pH to 6 by adding NaOH (0.1M) and HCl (0.1M) to prepare a mixed dispersion; wherein the concentration of the sodium alginate solution is 4.8%, and the concentration of the xanthan gum solution is 7.2%.

[0015] Preferably, in the above-mentioned composite camellia oil gel for treating burns and scalds and its preparation method, the polysaccharide gel solution is added to a mixture of monolaurin and camellia oil, homogenized at a speed of 13000 rpm / min for 3 minutes to form a uniform mixed solution; sheared at a speed of 10000 rpm / min for 3 minutes and then freeze-dried to obtain the composite camellia oil gel.

[0016] Preferably, in the above-mentioned composite camellia oil gel for treating burns and scalds and its preparation method, the freeze-drying time is 48 hours and the freeze-drying temperature is -58°C.

[0017] Preferably, the composite camellia oil gel for treating burns and scalds obtained by the above-mentioned preparation method is a composite oil gel for treating burns and scalds composed of camellia seed oil as a raw material.

[0018] Preferably, the present invention provides an application of a composite camellia oil gel in the preparation of a drug for preventing and treating burns and repairing scars, which can prevent persistent wound infection while alleviating inflammation in the body, increasing collagen deposition, and promoting angiogenesis, thereby promoting wound healing and reducing scar formation.

[0019] Therefore, the present invention adopts the above-mentioned composite camellia oil gel for treating burns and scalds and its preparation method and application. Camellia seeds are extracted and separated by cold pressing. Camellia seed oil is then combined with lauric acid monoglyceride having antibacterial and lipophilic properties to form a camellia oil gel mixture. The amphiphilic properties of lauric acid monoglyceride enable its fatty chain to be effectively embedded in oils and fats through hydrophobic interactions. At the same time, the hydroxyl group of lauric acid monoglyceride and the polar group or ester group in camellia oil produce weak interactions through hydrogen bonding. This local polar interaction helps lauric acid monoglyceride to form a dense three-dimensional network structure in camellia oil and endow it with excellent antibacterial ability.

[0020] In addition, xanthan gum and sodium alginate as polysaccharide oil gel can further effectively enhance the density of the three-dimensional network structure of the composite oil gel through hydrogen bonding and electrostatic interactions, significantly improving the gelling properties of the oil gel. The high adhesion of the composite camellia oil oil gel can more easily provide interface support for cell proliferation and migration, and can effectively inhibit the growth of bacteria near the wound surface, regulate the wound microenvironment, slow down inflammation and scar formation in the body, and achieve the effect of promoting wound healing.

[0021] A composite camellia oil gel encapsulating the antibacterial agent lauric acid monoglyceride is prepared, which is beneficial to skin penetration and wound healing. The singleness of the effects of oil, polysaccharide and monoglyceride on skin healing is solved, and the disadvantage of uncontrollable morphology is improved. By regulating the wound microenvironment, inflammation in the body is alleviated, collagen deposition is increased, angiogenesis is promoted, wound healing is promoted and scar formation is reduced. The method provided by the invention has mild and simple reaction conditions and is easy to industrialize.

[0022] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1The macroscopic and microscopic morphological diagrams of a composite camellia oil gel for treating burns and scalds of the present invention and its preparation method and application examples 1-3;

[0024] Figure 2 It is a graph showing the particle size distribution and oil holding capacity of a composite camellia oil gel for treating burns and scalds of the present invention and its preparation method and application examples 1-3;

[0025] Figure 3 This is a graph showing the thixotropic recovery ability of a composite camellia oil gel for treating burns and scalds of the present invention and its preparation method and application examples 1-3;

[0026] Figure 4 This is a diagram showing the antibacterial effects of a composite camellia oil gel for treating burns and scalds of the present invention, its preparation method, and application examples 1-3 and comparative example 1. DETAILED DESCRIPTION

[0027] In order to better understand the above technical solution, the above technical solution will be described in detail below in conjunction with the accompanying drawings and specific implementation methods. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] The terms used in the embodiments of the present invention are only for the purpose of describing specific embodiments, and are not intended to limit the present invention. The singular forms "a", "said" and "the" used in the embodiments of the present invention and the appended claims are also intended to include plural forms, unless the context clearly indicates other meanings, and "multiple" generally includes at least two.

[0029] It should also be noted that the term "includes", "comprising" or any other variation thereof is intended to cover non-exclusive inclusion, so that a commodity or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such commodity or device. In the absence of more restrictions, the elements defined by the sentence "comprising a ..." do not exclude the existence of other identical elements in the commodity or device including the elements.

[0030] Example 1

[0031] 1) Accurately weigh xanthan gum and sodium alginate, dissolve them in 10 g of deionized water respectively, and after they are completely dissolved, mix the fully hydrated sodium alginate solution and xanthan gum solution at a certain concentration, and finally adjust the pH to 6 to obtain xanthan gum-sodium alginate gel; the above operation ensures that the sodium alginate and xanthan gum in the system are 12 wt%, of which sodium alginate is 4.8 wt% and xanthan gum is 7.2 wt%.

[0032] 2) adding the xanthan gum-sodium alginate gel system of step 1) to 10 g of camellia oil at 80° C. and mixing thoroughly for 1 h; homogenizing at 10,000 rpm / min for 3 min to form a uniform mixed solution, and shearing at 10,000 rpm / min for 3 min; and freeze-drying at -58° C. for 48 h in a freeze dryer under vacuum conditions to obtain camellia oil gel.

[0033] Example 2

[0034] Accurately weigh 10g of camellia seed oil and add 0.7g of lauric acid monoglyceride, and continue stirring until it is completely dissolved; the stirring rate is 3000-5000rpm; the reaction conditions are 80°C for 0.5h; the obtained gel is filtered with a molecular weight 5-20kDa membrane, and the obtained filtrate is frozen for 24h at a freezing temperature of 4°C; the above operation ensures that the lauric acid monoglyceride is 7wt% in the system;

[0035] Example 3

[0036] 1) Accurately weigh xanthan gum and sodium alginate, dissolve them in 10 g of deionized water respectively, and after they are completely dissolved, mix the fully hydrated sodium alginate solution and xanthan gum solution at a certain concentration, and finally adjust the pH to 6 to obtain xanthan gum-sodium alginate gel; the above operation ensures that the sodium alginate and xanthan gum in the system are 12 wt%, of which sodium alginate is 4.8 wt% and xanthan gum is 7.2 wt%.

[0037] 2) Accurately weigh 10g of camellia seed oil and add 0.7g of lauric acid monoglyceride, and continue stirring until it is completely dissolved; the stirring rate is 3000-5000rpm; the reaction conditions are 80°C for 0.5h; the obtained gel is filtered with a molecular weight 5-20kDa membrane, and the obtained filtrate is frozen for 24h at a freezing temperature of 4°C; the above operation ensures that the lauric acid monoglyceride in the system is 7wt%;

[0038] 3) adding the xanthan gum-sodium alginate gel obtained in step 1) to the gel in step 2) at 80° C., mixing thoroughly for 1 h, homogenizing at 10,000 rpm / min for 3 min to form a uniform mixed solution, and then shearing at 10,000 rpm / min for 3 min; and freeze-drying at -58° C. for 48 h in a freeze dryer under vacuum conditions to obtain the composite camellia oil gel.

[0039] Comparative Example 1

[0040] The preparation method of Example 1 was followed, but lauric acid monoglyceride was not added in the steps, and the product obtained was single camellia seed oil as Comparative Example 1.

[0041] Comparative Example 2

[0042] Take Jin Wan Hong ointment, a commonly used burn ointment on the market, as comparative example 2.

[0043] Test 1: Macroscopic and microscopic morphology of camellia oil gel prepared in Examples 1-3

[0044] Method: The camellia oil gel obtained in Examples 1-3 was used as an experimental sample, which was placed in a sample bottle for macroscopic morphology observation and photographed. In addition, a polarizing microscope (PLM, Nikon AZ100) was used to observe its microstructure.

[0045] result: Figure 1 The macroscopic and microscopic structures of the oil gels of Examples 1-3. As can be seen from the figure, Examples 1-2 formed by adding monolaurin and polysaccharide gelling agents (sodium alginate and xanthan gum) all present an opaque gel, and the sticky touch gradually increases, which indicates that monolaurin and polysaccharide oil gelling agents can act as stabilizers in Examples 1-3. In addition, it can be found from the polarizing microscope (PLM) figure that the microstructure in Example 1 mainly presents an irregular lamellar structure, while Example 2 forms rose needle-shaped crystals with a three-dimensional network structure; and Example 3, due to the inclusion of the above-mentioned polyol gelling agent, has a microstructure that presents a coexistence crystal structure of rose needle-shaped and lamellar structures. Compared with Example 2, the rose needle-shaped structure of Example 3 is denser and more narrowly distributed. This shows that the dispersion of polysaccharides in Example 3 helps to form cross-linked crystal branches, which further interact with the oil gel, and finally form a denser and less anisotropic network structure.

[0046] Test 2: Determination of particle size and oil holding capacity of camellia oil gel prepared in Examples 1-3

[0047] Method: Take an appropriate amount of experimental sample, measure its particle size by dynamic light scattering instrument (LPSA, Bettersize BT-2600E), and measure oil holding capacity by centrifugation. The specific steps are: weigh the oil gel sample and centrifuge it at 8000rpm for 15min. After centrifugation, remove excess oil and weigh again. The calculation formula of oil holding capacity is as follows:

[0048]

[0049] result: Figure 2 The particle size distribution and oil holding capacity of the oil gel of Examples 1-3. The particle size distribution of all samples showed a multi-peak phenomenon, mainly concentrated in 127.3-198.5 μm. Among them, the particle size of Example 3 was significantly reduced, which may be due to the interaction between the hydrophilic group of monolaurin and the hydroxyl group in the polysaccharide gel. This effect promotes the dispersion ability of the polysaccharide gel in the oil gel, enhances the encapsulation of dispersed droplets, and causes the composite oil gel to have a smaller particle size. In addition, the oil holding capacity comparison results of Examples 1-3 show that the selection of the oil gel is crucial to the oil holding capacity of the composite oil gel. Anionic polysaccharide gels such as xanthan gum and sodium alginate are adsorbed on the surface of the oil body by increasing electrostatic deposition and spatial repulsion, forming a more solid structure and encapsulating more oil molecules. For Example 3, due to the introduction of monolaurin as a gel strengthener, not only the interaction between the polysaccharide gel and camellia oil is enhanced, but also the density of the three-dimensional network structure between monolaurin and camellia oil is improved. These effects can increase the contact specific surface area between the gelling agent and the oil, thereby effectively encapsulating more oil and slowing down the flow of liquid oil during centrifugation to a greater extent.

[0050] Test 3: Thixotropic recovery ability of camellia oil gel prepared in Examples 1-3

[0051] Method: The thixotropic recovery test was used to evaluate the viscosity recovery ability of camellia oil gel after shearing. First, the sample was allowed to stand for 5 minutes, and then the following steps were performed: 300s low shear rate (0.1s -1 ), 50s high shear rate (10s -1 ) and 300s low shear rate (0.1s -1 ). By comparing the final viscosity values ​​after the two low shear rate steps, the viscosity recovery percentage of the oleogel was calculated:

[0052]

[0053] result: Figure 3It is the thixotropic recovery curve of Examples 1-3 at different shear rates. It can be found from the figure that with the increase of shear rate, the apparent viscosity of the oil gel gradually decreases; and when the shear rate returns to a low rate, the apparent viscosity recovers rapidly. This phenomenon shows that the oil gel has good structural recovery ability. Comparing the initial viscosities of Example 1 and Example 3, it is found that the polysaccharide oil gel significantly improves the initial viscosity of the oil gel, further proving that the polysaccharide gel plays a leading role in the formation of the oil gel crystal network. The polysaccharide oil gel can increase the electrostatic repulsion of the oil gel, making its internal structure difficult to destroy. In addition, the addition of monolaurin enhances the crystallinity of Example 3, causing it to exhibit higher viscosity and elastic modulus.

[0054] Test 4: Evaluation of the antibacterial properties of the composite camellia oil gel prepared in Examples 1-3 and Comparative Example 1

[0055] Method: The blank control (ultrapure water, without any component added), comparative example 1 and examples 1-3 were used as experimental samples. 100 μL of the experimental sample was added to the logarithmic growth phase and the concentration was 10 6 Coli and Staphylococcus aureus suspensions with CFU / mL were spread on solid culture medium using a sterile spreading stick, and the culture dish was placed in a 37°C constant temperature incubator for 24 hours. The growth of colonies on the culture medium was observed and recorded.

[0056] Results: From Figure 4 It can be seen that Examples 1-3 and Comparative Example 1 have certain inhibitory effects on Escherichia coli and Staphylococcus aureus. Compared with Comparative Example 1 and Example 1, Examples 2-3 have significantly enhanced inhibitory effects on bacteria. Among them, the inhibition rate of Escherichia coli in Example 3 is almost 100%. It can be seen that the introduction of lauric acid monoglyceride in the present invention can not only serve as a gel strengthener to enhance the network structure of the oil gel, but also significantly improve the antibacterial activity of the oil gel. In addition, compared with Example 2, Example 3 has more significant inhibitory effects on Escherichia coli and Staphylococcus aureus, which may be due to the synergistic effect of the polysaccharide gelling agent and GML in the oil gel enhancing the inhibitory effect of the oil gel on bacteria.

[0057] Test 5: Application effects of camellia oil gel prepared in Examples 1-3 and Comparative Examples 1-2

[0058] Methods: 40 female SD mice (20-25 ± 5g) were selected and the experiment was carried out after 1 week of adaptability testing. The mice were anesthetized by intraperitoneal injection of chloral hydrate (4%, 200mg / kg), and the back hair was removed with a shaver, and 75% alcohol was used for heart disinfection. Subsequently, a scald probe with a temperature of 100°C was used to create a scald wound with a diameter of 8mm on the back of the mouse. The scald duration was 15s, and the external force applied was 500Pa. The mouse Ⅲ degree scald model was successfully constructed, and the wound was debrided. 1h after the scald, the experimental mice were randomly divided into 6 groups, each with 10 mice, namely: negative control group (normal saline group), drug administration group (Example 1-3 and Comparative Example 1), positive control group (Comparative Example 2). The experimental mice were caged and administered twice a day, with each mouse administered about 20mg. The scald wound conditions were recorded on the morning of the 1st, 4th, 9th and 13th days, and the scald wound area was measured. The wound healing rate was calculated according to the following formula:

[0059]

[0060] Results: As shown in Table 1, compared with the negative control group, Comparative Examples 1-2 and Examples 1-3 all had a certain effect on promoting the healing of the third degree burn wounds in mice. After 13 days of data comparison, it was found that the wound healing rate of Example 3 was significantly higher than that of the other groups, and its therapeutic effect was even comparable to that of the positive control (Comparative Example 2). This can be attributed to the interaction between camellia oil and various gelling agents, which accelerated the formation of new epidermis and provided a more favorable healing environment for the wound.

[0061] Table 1 Wound healing rate (x±s,%)

[0062] Group Day 4 Day 9 Day 13 Negative control 6.92±9.85 57.10±9.99 77.68±7.60 Comparative Example 1 16.55±8.02 62.51±3.94 83.41±5.77 Comparative Example 2 28.65±1.55 73.06±3.94 93.75±1.97 Example 1 19.06±0.70 66.69±1.41 86.31±2.81 Example 2 21.77±9.57 70.23±0.14 88.50±2.67 Example 3 32.03±0.14 77.48±5.35 96.06±0.56

[0063] Therefore, the present invention adopts the above-mentioned composite camellia oil gel for treating burns and scalds and its preparation method and application, and prepares a composite camellia oil gel encapsulating the antibacterial agent monolaurin, which is beneficial to skin penetration and wound healing. The singleness of the skin healing effect of oils, polysaccharides, and monoglycerides is solved, and the disadvantage of uncontrollable morphology is improved. By adjusting the wound microenvironment, inflammation in the body is slowed down, collagen deposition is increased, angiogenesis is promoted, wound healing is promoted, and scar formation is reduced. The method provided by the present invention has mild and simple reaction conditions and is easy to industrialize. In vitro antibacterial experiments and animal experiments have proved that it can promote wound healing by reducing bacterial infection. Compared with the traditional use of camellia oil, the present invention introduces functional components such as monolaurin, which significantly improves the application effect of oil gel. The preparation method of the composite camellia oil gel of the present invention is simple in process, high in stability, and suitable for industrial production; and the application effect is significant, and it has potential application prospects in the treatment of scald drugs. In addition, the present invention provides a theoretical basis for the application of camellia oil in the prevention and treatment of skin diseases and in the field of medical beauty, helping to improve the quality and efficiency of the camellia oil industry.

[0064] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. A method for preparing a composite camellia oil gel for treating burns and scalds, characterized in that: The composite camellia oil gel is prepared by taking camellia oil as a raw material, taking lauric acid monoglyceride as a gel intensifier and an antibacterial agent, and taking xanthan gum and sodium alginate as oil gelling agents; in different phase systems, the mass fraction of the camellia oil is 81wt%, the concentration of lauric acid monoglyceride is 7.0wt%, the concentration of the sodium alginate solution is 4.8%, and the concentration of the xanthan gum solution is 7.2%.

2. The method for preparing a composite camellia oil gel for treating burns and scalds according to claim 1, characterized in that: The steps include: The monolaurin is added to the camellia oil and stirred continuously until it is completely dissolved; the stirring rate is 3000-5000 rpm; the reaction condition is 80° C. for 0.5 h; The obtained mixture was filtered through a 5-20 kDa membrane, and the obtained filtrate was frozen overnight; the freezing time was 24 hours, and the freezing temperature was 4°C; preparing a polysaccharide xanthan gum solution and a sodium alginate solution, and mixing the polysaccharide xanthan gum solution and the sodium alginate solution to obtain a xanthan gum-sodium alginate polysaccharide gel; The polysaccharide gel solution is added into the mixture of lauric acid monoglyceride and camellia oil, and the composite camellia oil gel is obtained through homogenous mixing, shearing and freeze drying.

3. The method for preparing a composite camellia oil gel for treating burns and scalds according to claim 2, characterized in that: The xanthan gum is in liquid form to prepare a polysaccharide xanthan gum solution. The preparation method of the polysaccharide xanthan gum solution is as follows: dissolving the polysaccharide xanthan gum in deionized water and stirring it magnetically to fully hydrate the solution to obtain a xanthan gum solution of a certain concentration, cooling it to room temperature and then refrigerating it at 4°C.

4. The method for preparing a composite camellia oil gel for treating burns and scalds according to claim 3, characterized in that: The sodium alginate is in liquid form to prepare a sodium alginate solution. The preparation method of the sodium alginate solution is as follows: dissolving the sodium alginate in deionized water, heating in a water bath until it is completely dissolved to obtain a sodium alginate solution of a certain concentration, cooling to room temperature and then refrigerating at 4°C.

5. The method for preparing a composite camellia oil gel for treating burns and scalds according to claim 4, characterized in that: The method for constructing the xanthan gum-sodium alginate polysaccharide gel is as follows: fully mixing the polysaccharide xanthan gum and sodium alginate solution, and adjusting the pH to 6 by adding NaOH (0.1M) and HCl (0.1M) to prepare a mixed dispersion; wherein the concentration of the sodium alginate solution is 4.8%, and the concentration of the xanthan gum solution is 7.2%.

6. The method for preparing a composite camellia oil gel for treating burns and scalds according to claim 5, characterized in that: The polysaccharide gel solution is added to the mixture of monolaurin and camellia oil, homogenized at 13000 rpm / min for 3 minutes to form a uniform mixed solution; sheared at 10000 rpm / min for 3 minutes and freeze-dried to obtain composite camellia oil gel.

7. The method for preparing a composite camellia oil gel for treating burns and scalds according to claim 6, characterized in that: The freeze-drying time was 48 h and the freeze-drying temperature was -58 °C.

8. A composite camellia oil gel obtained by the preparation method according to any one of claims 1 to 7, characterized in that: The invention relates to a composite oil gel for treating burns and scalds, which is composed of camellia seed oil as raw material.

9. Use of the composite camellia oil gel according to claim 8 in preparing drugs for preventing and treating burns and repairing scars.

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