Composition for supplementing brain energy and application thereof

Abstract

The invention discloses a composition for supplementing brain energy and application thereof, and belongs to the technical field of biology. The invention discloses a composition for supplementing brain energy. The composition is prepared by compounding taurine, caffeine, a ginseng extract, an oolong tea extract, a wolfberry extract, a ginger extract, vitamin B1, vitamin B2, vitamin B6, vitamin B12, nicotinamide, pantothenic acid and biotin according to a certain proportion. The composition contains various components for supplementing brain energy, so that brain energy supply is guaranteed, neurotransmitters are regulated, and central fatigue is relieved; according to the invention, brain fatigue is effectively relieved by enhancing the cognition and reaction ability of the brain.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and more particularly to a composition for replenishing brain energy and its application. Background Art

[0002] With the advancement of social productivity, people's physical activities have decreased and their mental activities have increased. More and more people are facing the problem of brain fatigue. Central fatigue, also known as brain fatigue, refers to the weakening of the function of the central nervous system (mainly including the spinal cord and brain) after an individual has experienced long-term or high-intensity physical activities, which in turn leads to a decrease in muscle activation ability and athletic performance.

[0003] The mechanism of central fatigue involves many aspects, including changes in neurotransmitters, reduced brain energy metabolism, and decreased neuronal excitability. With the development of brain science and neuroscience technology and theory, how to prevent and relieve brain fatigue has become the focus of current research. "Brain energy", as the name suggests, refers to all beneficial factors that can enhance brain function and reduce brain fatigue. Studies have found that the key to relieving central fatigue lies in improving energy metabolism, regulating neurotransmitters, anti-oxidation, anti-inflammation, enhancing immune function, and regulating the endocrine system. Therefore, the method of supplementing "brain energy" is mainly to enhance brain function and reduce brain fatigue by means of improving energy metabolism and regulating neurotransmitters. At present, the commonly used anti-fatigue products are mostly foods such as coffee, betel nut, and tea. Although the excessive caffeine and arecoline contained in them can help people relieve fatigue to a certain extent, the excessive caffeine and arecoline contained in them are not conducive to the healthy development of the human body. Based on this, the development of new anti-fatigue formulas to achieve healthy, environmentally friendly and efficient anti-mental fatigue effects has become a new research direction.

[0004] Therefore, providing a composition for replenishing brain energy and its application is an urgent problem to be solved by those skilled in the art. Summary of the invention

[0005] In view of this, the present invention provides a composition for replenishing brain energy and its application, wherein the composition mainly improves cognitive ability and reaction ability by replenishing brain energy, thereby achieving the purpose of relieving mental fatigue.

[0006] In order to achieve the above object, the present invention adopts the following technical solution:

[0007] A composition for replenishing brain energy, comprising the following raw materials in parts by weight: 50 parts of taurine, 20 parts of caffeine, 5 parts of ginseng extract, 5 parts of oolong tea extract, 5 parts of wolfberry extract, 5 parts of ginger extract, 1 part of vitamin B1, 0.5 parts of vitamin B2, 0.5 parts of vitamin B6, and 0.5 parts of vitamin B 120.001 parts, niacinamide 5 parts, pantothenic acid 1 part, biotin 0.005 parts.

[0008] The weight parts described in the present invention may be known content units such as μg, mg, g, kg, etc.

[0009] Furthermore, a method for preparing a composition for replenishing brain energy comprises adding solid powdered taurine, caffeine, ginseng extract, oolong tea extract, wolfberry extract, ginger extract, vitamin B1, vitamin B2, vitamin B6, vitamin B 12 , niacinamide, pantothenic acid and biotin are mixed to form a composition.

[0010] Furthermore, the composition for supplementing brain energy is used to enhance the cognitive ability and reaction ability of the brain.

[0011] Furthermore, the composition for supplementing brain energy is used in relieving mental fatigue.

[0012] It can be seen from the above technical solutions that, compared with the prior art, the present invention discloses a composition for supplementing brain energy and its application, which has the following beneficial effects:

[0013] The composition for supplementing brain energy provided by the present invention comprises taurine, caffeine, ginseng extract, oolong tea extract, wolfberry extract, ginger extract, vitamin B1, vitamin B2, vitamin B6, vitamin B 12 , niacinamide, pantothenic acid, and biotin are compounded in a specific proportion. Through the synergistic effect of the above raw materials and the scientific formula, it has the triple effects of "increasing energy supply, optimizing energy environment, and providing mental energy". Each component plays its role synergistically to enhance brain function and ultimately achieve the purpose of relieving brain fatigue.

[0014] Enhance energy supply: Taurine can promote the metabolism and oxidation of fat, and participate in energy metabolism together with B vitamins to promote the production of brain energy substances. Ginseng extract and wolfberry extract promote brain energy metabolism by increasing adenosine triphosphate levels and enhancing adenosine triphosphatase activity. Caffeine can inhibit phosphodiesterase activity, increase brain energy consumption, heat production and fat oxidation.

[0015] Optimize the energy environment: Ginseng, oolong tea, wolfberry and ginger extracts can reduce oxidative stress and inflammatory responses in the brain, thereby protecting neurons. B vitamins participate in the body's oxidation / antioxidant system to maintain brain homeostasis. Caffeine protects brain function by neutralizing free radicals and inhibiting the release of inflammatory mediators, reducing oxidative stress and inflammatory responses in the brain.

[0016] Provide mental energy: Taurine, B vitamins, ginseng extract, wolfberry extract, and caffeine improve energy metabolism. Ginseng extract, oolong tea extract, B vitamins, and caffeine also participate in neurotransmitter regulation and improve brain cognitive function. Wolfberry, oolong tea, and ginger extract help strengthen the immune system, while oolong tea extract can regulate endocrine, thereby reducing brain fatigue. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0018] Figure 1 The accompanying figure is a typical diagram of the movement trajectory of zebrafish after the sample is treated according to the present invention;

[0019] A: Physical picture of the plus maze; Note: The blue box is the blue area of ​​the plus maze, which is the quantitative area; B: Typical picture of the movement trajectory of zebrafish;

[0020] Figure 2 The attached figure shows the movement proportion of the blue area of ​​zebrafish after the sample of the present invention is treated; compared with the model control group, *** p<0.001; compared with comparative example 1, # p<0.05;

[0021] Figure 3 The attached figure shows the light-dark speed difference per minute of zebrafish after treatment with the samples of the present invention; compared with the model control group, *p<0.05, **p<0.01, ***p<0.001; compared with comparative example 1, # p<0.05. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Dimethyl sulfoxide: DMSO, batch number I2229063, Shanghai Aladdin Biochemical Technology Co., Ltd., China. Rice bran fatty alkyl alcohol, white powder, batch number HH0837, Baishi (Shanghai) Biotechnology Co., Ltd.; the solvent is DMSO.

[0024] Taurine was purchased from Qianjiang Yongan Pharmaceutical Co., Ltd.; caffeine was purchased from Shandong Xinhua Pharmaceutical Co., Ltd.; ginseng extract, wolfberry extract, and ginger extract were purchased from Shaanxi Jiahe Biotechnology Co., Ltd.; oolong tea extract was purchased from Fujian Zhongchun Biotechnology Co., Ltd.; vitamin B1 and vitamin B6 were purchased from Jiangxi Tianxin Pharmaceutical Co., Ltd.; vitamin B2 was purchased from Chifeng Pharmaceutical Co., Ltd.; vitamin B 12 purchased from Zhengzhou Ruipu Bioengineering Co., Ltd.; niacinamide purchased from Jiangxi Brothers Pharmaceutical; pantothenic acid purchased from Nantong Licheng Bioengineering Co., Ltd.; biotin purchased from Wuhu Meno Food Technology Co., Ltd.

[0025] Embodiment 1

[0026] A composition for replenishing brain energy, the composition of which is as follows: 50 mg of taurine, 20 mg of caffeine, 5 mg of ginseng extract, 5 mg of oolong tea extract, 5 mg of wolfberry extract, 5 mg of ginger extract, 1 mg of vitamin B1, 0.5 mg of vitamin B2, 0.5 mg of vitamin B6, and 0.5 mg of vitamin B 12 1μg, niacinamide 5mg, pantothenic acid 1mg, biotin 5μg.

[0027] Preparation method of a composition for replenishing brain energy: weigh raw materials according to the ratio, add solid powdered taurine, caffeine, ginseng extract, oolong tea extract, wolfberry extract, ginger extract, vitamin B1, vitamin B2, vitamin B6, vitamin B 12 , niacinamide, pantothenic acid, and biotin are mixed to form a mixture.

[0028] Comparative Example 1

[0029] A composition for supplementing brain energy, the composition of which is as follows: 40 mg of ginseng extract, 40 mg of oolong tea extract, 50 mg of wolfberry extract and 50 mg of ginger extract.

[0030] The preparation method of the composition for replenishing brain energy is as follows: raw materials are weighed according to the proportion, and solid powdered ginseng extract, oolong tea extract, wolfberry extract and ginger extract are mixed to prepare a mixture.

[0031] Comparative Example 2

[0032] A composition for supplementing brain energy, the composition of which is as follows: 300 mg of taurine and 100 mg of caffeine.

[0033] The preparation method of the composition for supplementing brain energy is as follows: raw materials are weighed according to a ratio, and solid powdered taurine and caffeine are mixed to prepare a mixture.

[0034] Comparative Example 3

[0035] A composition for supplementing brain energy, the composition used is as follows: vitamin B14mg, vitamin B22mg, vitamin B62mg, vitamin B 12 4μg, niacinamide 20mg, pantothenic acid 7mg, biotin 50μg.

[0036] The preparation method of the composition for replenishing brain energy is as follows: weigh the raw materials according to the proportion, mix solid powdered vitamin B1, vitamin B2, vitamin B6, vitamin B 12 , niacinamide, pantothenic acid, and biotin are mixed to form a mixture.

[0037] Experimental example: Experiment on the efficacy of relieving mental fatigue

[0038] 1. Experimental subjects and methods

[0039] 1.1 Animal Experimental Subjects

[0040] Zebrafish were raised in fish farming water at 28°C (water quality: 200 mg of instant sea salt was added to every 1 L of reverse osmosis water, conductivity was 450-550 μS / cm; pH was 6.5-8.5; hardness was 50-100 mg / LCaCO3), the experimental animal use license number is: SYXK (Zhejiang) 2022-0004, and the breeding management complies with the requirements of the international AAALAC certification (certification number: 001458), IACUC ethics review number: IACUC-2024-10200-01.

[0041] Wild-type AB strain zebrafish were set up with a normal control group, a model group, a positive control group (rice bran fatty alkanol 1000 μg / mL concentration), and an experimental group (Example 1, Comparative Examples 1-3). Except for the normal control group, the zebrafish in the remaining groups were stimulated with light and dark for 6 hours in a behavior instrument, and a zebrafish mental fatigue model was induced by sleep deprivation.

[0042] 1.2 Evaluation indicators

[0043] (1) Blue area ratio of the cross maze (cognitive ability)

[0044] After the samples have been treated for a period of time, a behavior analyzer is used to observe the behavioral changes of zebrafish, and the statistical analysis results of this indicator are used to evaluate the effectiveness of the samples in relieving mental fatigue.

[0045] (2) Difference in light and dark speed per minute (reaction ability)

[0046] After the samples have been treated for a period of time, a behavior analyzer is used to observe the difference in light and dark speed per minute in the zebrafish, and the statistical analysis results of this indicator are used to evaluate the effectiveness of the samples in relieving mental fatigue.

[0047] 1.3 Experimental methods

[0048] (1) Maximum detectable concentration (MTC) determination

[0049] Wild-type AB zebrafish at 4 days post-fertilization (dpf) were randomly selected in a 6-well plate, and 30 zebrafish were treated in each well (experimental group). The samples were given in water (concentrations are shown in Table 1), and a normal control group and a model control group were set up at the same time, with a capacity of 3 mL per well. After 1 day of treatment at 28°C, except for the normal control group, all other experimental groups were stimulated with light and dark for 6 hours in the behavior instrument (the program was set as: 100% white light for 20s, 0% white light (darkness) for 5s, 100% white light for 15s, 0% white light (darkness) for 3s, and cycle stimulation for 6h) to establish a zebrafish mental fatigue model. The MTC of the samples on the model zebrafish was determined.

[0050] (2) Relieve mental fatigue (improve cognitive ability)

[0051] 4dpf wild-type AB strain zebrafish were randomly selected in a 6-well plate, and 30 zebrafish were treated in each well (experimental group). Water-soluble samples (concentrations are shown in Table 2), and normal control group and model control group were set up at the same time. The positive control rice bran fatty alkyl alcohol concentration was 1000μg / mL, and the capacity of each well was 3mL. After 1 day of treatment at 28℃, except for the normal control group, the rest of the experimental groups were stimulated by light and dark for 6 hours in the behavior instrument to establish the zebrafish mental fatigue model. Five zebrafish were randomly selected from each experimental group and placed in the "cross" module. The module was divided into four areas: yellow, blue, red and green. Six modules were placed in each group. Data were collected using a behavior analyzer to analyze the percentage (%) of the total movement distance of zebrafish in the blue area within 10 minutes to the total movement distance of the entire area. The statistical analysis results of this indicator were used to evaluate the efficacy of the sample in relieving mental (spiritual) fatigue.

[0052] (3) Relieve mental fatigue (improve reaction ability)

[0053] 4dpf wild-type AB strain zebrafish were randomly selected in a 6-well plate, and 30 zebrafish were treated in each well (experimental group). Water-soluble samples (concentrations are shown in Table 3), normal control group and model control group were set up at the same time, and the positive control rice bran fatty alkyl alcohol concentration was 1000μg / mL, and the capacity of each well was 3mL. After 1 day of treatment at 28℃, except for the normal control group, the rest of the experimental groups were stimulated by light and dark for 6 hours in the behavior instrument to establish the zebrafish mental fatigue model. 10 zebrafish were randomly selected from each experimental group, and the average movement speed per minute of zebrafish under light and dark stimulation was measured using a behavior analyzer. After calculation, the statistical analysis results of the difference in light and dark speed per minute were used to evaluate the efficacy of the samples in relieving mental (spiritual) fatigue.

[0054] 1.4 Results Statistics

[0055] The statistical results were expressed as mean ± SE. SPSS 26.0 software was used for statistical analysis, and p < 0.05 indicated that the difference was statistically significant.

[0056] 2. Experimental results

[0057] 2.1MTC

[0058] Under the experimental conditions, the MTCs for relieving mental fatigue in Example 1 and Comparative Examples 1-3 were 250, 500, 125 and 1000 μg / mL. See Table 1 for details.

[0059] Table 1 Results of the experimental study on the concentration of samples for relieving mental fatigue (n=30)

[0060]

[0061] 2.2 Relieve mental fatigue (improve cognitive ability)

[0062] Under the experimental conditions, Example 1 and Comparative Examples 1-3 all have the effect of relieving mental fatigue, which is specifically manifested in improving cognitive ability. See Table 2 for details. Figure 1 and Figure 2 .

[0063] Table 2 Results of the experimental evaluation of the efficacy of samples in relieving mental fatigue (n=6)

[0064]

[0065]

[0066] Note: Compared with the model control group, ***p<0.001; compared with comparative example 1, # p<0.05.

[0067] 2.3 Relieve mental fatigue (improve reaction ability)

[0068] Under the experimental conditions, Example 1 and Comparative Examples 1-3 all have the effect of relieving mental fatigue, which is specifically manifested in improving reaction ability. See Table 3, Table 4 and Figure 3 .

[0069] The behavior analyzer can automatically calculate and display the total movement distance per minute; average speed per minute = total movement distance per minute / 60s; reaction ability (difference between light and dark speed per minute) = average speed per minute in dark environment - average speed per minute in light environment.

[0070] Table 3 Samples improving responsiveness efficacy evaluation test results 1 (n = 10)

[0071]

[0072] Table 4 Samples improve responsiveness and efficacy evaluation test results 2 (n = 10)

[0073]

[0074] Note: Compared with the model control group, *p<0.05, **p<0.01, ***p<0.001; compared with comparative example 1, # p<0.05.

[0075] 3. Conclusion

[0076] The present invention provides a composition for supplementing brain energy. The composition of Example 1 comprises taurine, caffeine, ginseng extract, oolong tea extract, wolfberry extract, ginger extract, vitamin B1, vitamin B2, vitamin B6, vitamin B 12 , niacinamide, pantothenic acid, biotin. The experimental results show that Example 1 is most effective in relieving mental fatigue. Comparative Example 1 mainly contains a variety of extracts, such as ginseng extract, oolong tea extract, wolfberry extract, and ginger extract. Comparative Example 2 mainly contains taurine and caffeine. Comparative Example 3 mainly contains B vitamins, including vitamin B1, vitamin B2, vitamin B6, vitamin B 12 , nicotinamide, pantothenic acid, biotin. The experimental results show that comparative examples 1-3 also have the effect of relieving mental fatigue, but the effects are not as good as those of embodiment 1.

[0077] In summary, the ingredients of the present invention include taurine, caffeine, ginseng extract, oolong tea extract, wolfberry extract, ginger extract, vitamin B1, vitamin B2, vitamin B6, vitamin B 12 After nicotinamide, pantothenic acid, and biotin enter the body's metabolic system, they can effectively enhance brain function and relieve mental fatigue. After using multiple ingredients in combination, it can more significantly replenish brain energy and further enhance the effect of relieving mental fatigue.

[0078] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A composition for supplementing brain energy, characterized in that: Contains the following raw materials by weight: 50 parts of taurine, 20 parts of caffeine, 5 parts of ginseng extract, 5 parts of oolong tea extract, 5 parts of wolfberry extract, 5 parts of ginger extract, 1 part of vitamin B1, 0.5 parts of vitamin B2, 0.5 parts of vitamin B6, and 0.5 parts of vitamin B 12 0.001 parts, niacinamide 5 parts, pantothenic acid 1 part, biotin 0.005 parts.

2. The method for preparing a composition for supplementing brain energy according to claim 1, characterized in that: According to the weight parts of claim 1, solid powdered taurine, caffeine, ginseng extract, oolong tea extract, wolfberry extract, ginger extract, vitamin B1, vitamin B2, vitamin B6, vitamin B 12 , niacinamide, pantothenic acid and biotin are mixed to form a composition.

3. Use of the composition for supplementing brain energy as claimed in claim 1 in enhancing brain cognitive ability and reaction ability.

4. Use of the composition for supplementing brain energy according to claim 1 in relieving mental fatigue.

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