Medical dressing containing collagen and preparation method

Abstract

The invention relates to the technical field of medical dressings, and discloses a medical dressing containing collagen and a preparation method thereof.The dressing is prepared from, by weight, 1%-12% of collagen, 1%-3% of biocompatible base material, 2%-5% of ceramide, 1%-3% of hyaluronic acid, 0.5%-6% of plant extract, 0.1%-1% of carbomer, 1%-3% of glycerinum, 2%-5% of propylene glycol and the balance deionized water. The plant extract is selected from a green tea extract, a licorice extract, a centella asiatica extract and a ginkgo leaf extract. The preparation method comprises the following steps: dissolving the biocompatible base material, carbomer, glycerol and propylene glycol in purified water, then adding collagen, ceramide, hyaluronic acid and plant extract, fully and uniformly stirring, filtering, and finally sterilizing. By optimizing the pretreatment and preparation process of the collagen, the biocompatibility, the stability and the multifunctionality of the medical dressing are remarkably improved, and the medical dressing is suitable for treating various skin problems.

Description

Technical Field

[0001] The invention relates to the technical field of medical dressings, and in particular to a medical dressing containing collagen and a preparation method thereof. Background Art

[0002] With the continuous development of biomedical technology, medical dressings not only need to have the basic function of protecting wounds, but also gradually develop in the direction of promoting wound healing, reducing scar formation and other multifunctional directions. Collagen, as one of the most abundant proteins in the human body, has a significant effect in promoting wound healing due to its good biocompatibility and the role of promoting cell growth. However, the existing medical dressings containing collagen have deficiencies in the stability and release efficiency of collagen, which limits its clinical application. In addition, traditional medical dressings often lack sufficient biocompatibility and multifunctionality, and it is difficult to meet the treatment needs of complex wounds. Therefore, the development of a medical dressing that can improve the stability and dispersibility of collagen, enhance biocompatibility and biological activity, and have the synergistic effect of multiple plant extracts has become an important direction of current research. Therefore, we propose a medical dressing containing collagen and a preparation method. Summary of the invention

[0003] The main purpose of the present invention is to provide a medical dressing containing collagen and a preparation method, which can effectively solve the problems in the background technology.

[0004] To achieve the above object, the technical solution adopted by the present invention is:

[0005] A medical dressing containing collagen is composed of the following raw materials in percentage by weight: 1% to 12% collagen, 1% to 3% biocompatible substrate, 2% to 5% ceramide, 1% to 3% hyaluronic acid, 0.5% to 6% plant extract, 0.1% to 1% carbomer, 1% to 3% glycerol, 2% to 5% propylene glycol, and the balance is purified water.

[0006] Preferably, the biocompatible substrate is selected from one or a combination of polylactic acid and chitosan.

[0007] Preferably, the collagen is selected from high-purity natural collagen that has undergone multiple steps of pretreatment, and the specific pretreatment steps are:

[0008] A: Use ultra-fine grinding equipment to refine high-purity natural collagen particles and increase their surface area to obtain natural collagen powder;

[0009] B: Use protease to partially hydrolyze the natural collagen powder to generate smaller peptides, filter and centrifuge, and take the supernatant for later use to obtain a natural collagen solution;

[0010] C: Add an appropriate amount of surfactant to the collagen solution to reduce surface tension and improve the dispersibility and stability of collagen.

[0011] Preferably, in step B, the protease is selected from any one of papain and trypsin.

[0012] Preferably, in step C, the surfactant is selected from Tween-80.

[0013] Preferably, the natural collagen is selected from any one of fish collagen and marine organism collagen.

[0014] Preferably, the plant extract is selected from green tea extract, licorice extract, Centella asiatica extract and ginkgo leaf extract.

[0015] Preferably, the mass ratio of the green tea extract, the licorice extract, the Centella asiatica extract and the ginkgo leaf extract is 1:3:2:1.

[0016] The present invention also discloses a method for preparing a medical dressing containing collagen, comprising the following steps:

[0017] S1: Weigh a required amount of purified water, pour it into a stirring container, slowly add the uniformly mixed biocompatible substrate, carbomer, glycerin and propylene glycol into the purified water while stirring, place the stirring container on a heating device, gradually heat it to 80-85° C., and continue stirring until the biocompatible substrate and carbomer are completely dissolved, to obtain a first mixed solution;

[0018] S2: Cool the first mixed solution to 40-45° C., slowly add collagen, ceramide, hyaluronic acid and plant extract to the first mixed solution, and continue stirring to ensure that all ingredients are fully mixed, then add the remaining amount of purified water to the mixed solution, and continue stirring until all ingredients are completely dissolved to obtain a uniform second mixed solution;

[0019] S3: Filter the second mixed liquid through a 300-mesh filter cloth to remove undissolved particles and other impurities to ensure the purity of the mixed liquid;

[0020] S4: Filling the filtered second mixed liquid into a sterile container and sterilizing the filled medical dressing.

[0021] Preferably, the sterilization treatment is performed by gamma-ray irradiation sterilization.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. The present invention increases the surface area of ​​collagen by ultrafine grinding, enzymatic hydrolysis and surfactant treatment. Ultrafine grinding makes it easier to dissolve and disperse. Papain or trypsin is used to partially hydrolyze the collagen to generate smaller peptide segments, thereby improving its solubility and bioavailability. The addition of Tween-80 surfactant reduces the surface tension, further improves the dispersibility and stability of collagen, makes the distribution of collagen in medical dressings more uniform, and improves its absorption efficiency on the skin, thereby better playing its role in promoting wound healing and reducing scar formation.

[0024] 2. The present invention selects polylactic acid and chitosan as biocompatible substrates. Both materials have good biocompatibility and biodegradability, can be well combined with human tissues, and reduce immune response and allergic risks. Fish collagen and marine collagen are selected. The collagen from these sources has low immunogenicity, is similar to the structure of human collagen, and has better biocompatibility and biological activity. The combined use of these materials makes the medical dressing more gentle when it contacts the skin, reduces irritation and allergic reactions, and enhances its ability to promote cell proliferation and wound healing.

[0025] 3. The present invention adds green tea extract, licorice extract, Centella asiatica extract and ginkgo leaf extract, and these plant extracts have multiple bioactive ingredients, such as antioxidants, anti-inflammatory agents and antibacterial agents. Green tea extract has powerful antioxidant and anti-inflammatory effects, can reduce inflammatory reactions and promote wound healing; licorice extract has anti-inflammatory, anti-allergic and whitening effects, can relieve skin inflammation and promote wound healing; Centella asiatica extract promotes collagen synthesis, anti-inflammatory and anti-oxidation, accelerates wound healing and reduces scar formation; ginkgo leaf extract promotes blood circulation, anti-oxidation and anti-inflammatory, and accelerates wound healing. The synergistic effect of these plant extracts makes the medical dressing have stronger repair, anti-inflammatory, antioxidant and antibacterial effects, and is suitable for the treatment of various skin problems, such as trauma, burns, acne and postoperative care.

[0026] 4. The preparation method of the medical dressing containing collagen provided by the present invention ensures the uniform mixing and complete dissolution of each component by precisely controlling the temperature, stirring time and filtering steps, strictly controls the temperature during heating and cooling to avoid component denaturation and failure, ensures that all components are fully mixed, avoids stratification and precipitation, and uses 300 mesh filter cloth to filter to remove undissolved particles and other impurities, thereby ensuring the purity of the final product. Gamma-ray irradiation sterilization is used, which is an efficient and residue-free sterilization method that can completely kill bacteria, viruses and other microorganisms, ensure the sterility of medical dressings, and avoid infection risks. Strict preparation and sterilization processes ensure the safety and effectiveness of medical dressings, making them more reliable in clinical applications and suitable for various medical scenarios requiring a sterile environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 The present invention is a flow chart of the preparation of a medical dressing containing collagen. DETAILED DESCRIPTION

[0028] Example 1

[0029] like Figure 1 As shown, a medical dressing containing collagen is composed of the following raw materials in weight percentage: 1% collagen, 1% biocompatible matrix, 2% ceramide, 1% hyaluronic acid, 0.5% plant extract, 0.1% carbomer, 1% glycerol, 2% propylene glycol, and the balance is purified water.

[0030] Wherein, the biocompatible substrate is selected from one or a combination of polylactic acid and chitosan.

[0031] Wherein, the collagen is selected from high-purity natural collagen after multi-step pretreatment, and the specific pretreatment steps are:

[0032] A: Use ultra-fine grinding equipment to refine high-purity natural collagen particles and increase their surface area to obtain natural collagen powder;

[0033] B: Use protease to partially hydrolyze the natural collagen powder to generate smaller peptides, filter and centrifuge, and take the supernatant for later use to obtain a natural collagen solution;

[0034] C: Add an appropriate amount of surfactant to the collagen solution to reduce surface tension and improve the dispersibility and stability of collagen.

[0035] Wherein, the protease is selected from any one of papain and trypsin.

[0036] Wherein, the surfactant is selected from Tween-80.

[0037] Wherein, the natural collagen is selected from any one of fish collagen and marine organism collagen.

[0038] Wherein, the plant extract is selected from green tea extract, licorice extract, Centella asiatica extract and ginkgo leaf extract, and the mass ratio of green tea extract, licorice extract, Centella asiatica extract and ginkgo leaf extract is 1:3:2:1.

[0039] The preparation method of the medical dressing containing collagen comprises the following steps:

[0040] S1: Weigh a required amount of purified water, pour it into a stirring container, slowly add the uniformly mixed biocompatible substrate, carbomer, glycerin and propylene glycol into the purified water while stirring, place the stirring container on a heating device, gradually heat it to 80-85° C., and continue stirring until the biocompatible substrate and carbomer are completely dissolved, to obtain a first mixed solution;

[0041] S2: Cool the first mixed solution to 40-45° C., slowly add collagen, ceramide, hyaluronic acid and plant extract to the first mixed solution, and continue stirring to ensure that all ingredients are fully mixed, then add the remaining amount of purified water to the mixed solution, and continue stirring until all ingredients are completely dissolved to obtain a uniform second mixed solution;

[0042] S3: Filter the second mixed liquid through a 300-mesh filter cloth to remove undissolved particles and other impurities to ensure the purity of the mixed liquid;

[0043] S4: Filling the filtered second mixed liquid into a sterile container, and sterilizing the filled medical dressing by gamma-ray irradiation sterilization.

[0044] Example 2

[0045] like Figure 1 As shown, a medical dressing containing collagen is composed of the following raw materials in weight percentage: 12% collagen, 3% biocompatible matrix, 5% ceramide, 3% hyaluronic acid, 6% plant extract, 1% carbomer, 3% glycerol, 5% propylene glycol, and the balance is purified water.

[0046] Wherein, the biocompatible substrate is selected from one or a combination of polylactic acid and chitosan.

[0047] Wherein, the collagen is selected from high-purity natural collagen after multi-step pretreatment, and the specific pretreatment steps are:

[0048] A: Use ultra-fine grinding equipment to refine high-purity natural collagen particles and increase their surface area to obtain natural collagen powder;

[0049] B: Use protease to partially hydrolyze the natural collagen powder to generate smaller peptides, filter and centrifuge, and take the supernatant for later use to obtain a natural collagen solution;

[0050] C: Add an appropriate amount of surfactant to the collagen solution to reduce surface tension and improve the dispersibility and stability of collagen.

[0051] Wherein, the protease is selected from any one of papain and trypsin.

[0052] Wherein, the surfactant is selected from Tween-80.

[0053] Wherein, the natural collagen is selected from any one of fish collagen and marine organism collagen.

[0054] Wherein, the plant extract is selected from green tea extract, licorice extract, Centella asiatica extract and ginkgo leaf extract, and the mass ratio of green tea extract, licorice extract, Centella asiatica extract and ginkgo leaf extract is 1:3:2:1.

[0055] The preparation method of the medical dressing containing collagen comprises the following steps:

[0056] S1: Weigh a required amount of purified water, pour it into a stirring container, slowly add the uniformly mixed biocompatible substrate, carbomer, glycerin and propylene glycol into the purified water while stirring, place the stirring container on a heating device, gradually heat it to 80-85° C., and continue stirring until the biocompatible substrate and carbomer are completely dissolved, to obtain a first mixed solution;

[0057] S2: Cool the first mixed solution to 40-45° C., slowly add collagen, ceramide, hyaluronic acid and plant extract to the first mixed solution, and continue stirring to ensure that all ingredients are fully mixed, then add the remaining amount of purified water to the mixed solution, and continue stirring until all ingredients are completely dissolved to obtain a uniform second mixed solution;

[0058] S3: Filter the second mixed liquid through a 300-mesh filter cloth to remove undissolved particles and other impurities to ensure the purity of the mixed liquid;

[0059] S4: Filling the filtered second mixed liquid into a sterile container, and sterilizing the filled medical dressing by gamma-ray irradiation sterilization.

[0060] Example 3

[0061] like Figure 1 As shown, a medical dressing containing collagen is composed of the following raw materials in weight percentage: 7% collagen, 2% biocompatible matrix, 4% ceramide, 2% hyaluronic acid, 3% plant extract, 0.5% carbomer, 2% glycerol, 3% propylene glycol, and the balance is purified water.

[0062] Wherein, the biocompatible substrate is selected from one or a combination of polylactic acid and chitosan.

[0063] Wherein, the collagen is selected from high-purity natural collagen after multi-step pretreatment, and the specific pretreatment steps are:

[0064] A: Use ultra-fine grinding equipment to refine high-purity natural collagen particles and increase their surface area to obtain natural collagen powder;

[0065] B: Use protease to partially hydrolyze the natural collagen powder to generate smaller peptides, filter and centrifuge, and take the supernatant for later use to obtain a natural collagen solution;

[0066] C: Add an appropriate amount of surfactant to the collagen solution to reduce surface tension and improve the dispersibility and stability of collagen.

[0067] Wherein, the protease is selected from any one of papain and trypsin.

[0068] Wherein, the surfactant is selected from Tween-80.

[0069] Wherein, the natural collagen is selected from any one of fish collagen and marine organism collagen.

[0070] Wherein, the plant extract is selected from green tea extract, licorice extract, Centella asiatica extract and ginkgo leaf extract, and the mass ratio of green tea extract, licorice extract, Centella asiatica extract and ginkgo leaf extract is 1:3:2:1.

[0071] The preparation method of the medical dressing containing collagen comprises the following steps:

[0072] S1: Weigh a required amount of purified water, pour it into a stirring container, slowly add the uniformly mixed biocompatible substrate, carbomer, glycerin and propylene glycol into the purified water while stirring, place the stirring container on a heating device, gradually heat it to 80-85° C., and continue stirring until the biocompatible substrate and carbomer are completely dissolved, to obtain a first mixed solution;

[0073] S2: Cool the first mixed solution to 40-45° C., slowly add collagen, ceramide, hyaluronic acid and plant extract to the first mixed solution, and continue stirring to ensure that all ingredients are fully mixed, then add the remaining amount of purified water to the mixed solution, and continue stirring until all ingredients are completely dissolved to obtain a uniform second mixed solution;

[0074] S3: Filter the second mixed liquid through a 300-mesh filter cloth to remove undissolved particles and other impurities to ensure the purity of the mixed liquid;

[0075] S4: Filling the filtered second mixed liquid into a sterile container, and sterilizing the filled medical dressing by gamma-ray irradiation sterilization.

[0076] Experimental Group

[0077] 1. The medical dressings containing collagen prepared in the above three embodiments and medical dressings selected from the market were subjected to closed sensitization test, and the test results are as follows:

[0078] Experimental Project Example 1 Example 2 Example 3 Other 1 Other 2 Skin irritation index 0 0 0 2 5 Skin sensitization none none none have none

[0079] It can be seen from the above data that the medical dressing containing collagen of the present invention does not irritate the skin and does not cause allergic reactions in the skin.

[0080] 2. Comparative test on the antibacterial effect of the medical dressings containing collagen prepared in the above three embodiments and medical dressings selected from the market on wound bacteria was conducted. The test results are as follows:

[0081] Bacterial samples Example 1 Example 2 Example 3 Other 1 Other 2 Staphylococcus aureus 86.8％ 87.2％ 91.6％ 69.4％ 72.3％ Escherichia coli 85.1％ 83.7％ 89.2％ 68.9％ 61.3％ Candida albicans 86.1％ 85.2％ 90.3％ 65.6％ 63.5％

[0082] It can be seen from the above data that the medical dressing containing collagen of the present invention has an excellent inhibitory effect on Escherichia coli, Staphylococcus aureus and Candida albicans, and has a good antibacterial effect.

[0083] In addition, the medical dressing containing collagen of the present invention increases the surface area of ​​the collagen through ultrafine grinding, enzymatic hydrolysis and surfactant treatment. The ultrafine grinding makes it easier to dissolve and disperse. The collagen is partially hydrolyzed by papain or trypsin to generate smaller peptide segments, thereby improving its solubility and bioavailability. The addition of Tween-80 surfactant reduces the surface tension, further improves the dispersibility and stability of the collagen, makes the distribution of the collagen in the medical dressing more uniform, and improves its absorption efficiency on the skin, thereby better playing its role in promoting wound healing and reducing scar formation. Polylactic acid and chitosan are selected as biocompatible substrates. Both materials have good biocompatibility and biodegradability, can be well combined with human tissues, and reduce immune response and allergy risks. Fish collagen and marine collagen are selected. Collagen from these sources has low immunogenicity, is similar to human collagen structure, and has better biocompatibility and bioactivity. The combined use of these materials makes the medical dressings gentler when in contact with the skin, reduces irritation and allergic reactions, and enhances its ability to promote cell proliferation and wound healing. Green tea extract, licorice extract, Centella asiatica extract and ginkgo leaf extract are added. These plant extracts have a variety of bioactive ingredients, such as antioxidants, anti-inflammatory agents and antibacterial agents. Green tea extract has powerful antioxidant and anti-inflammatory effects, which can reduce inflammatory reactions and promote wound healing; licorice extract has anti-inflammatory, anti-allergic and whitening effects, which can relieve skin inflammation and promote wound healing; Centella asiatica extract promotes collagen synthesis, anti-inflammatory and anti-oxidation, accelerates wound healing and reduces scar formation; ginkgo leaf extract promotes blood circulation, anti-oxidation and anti-inflammatory, and accelerates wound healing. The synergistic effect of these plant extracts makes the medical dressing have stronger repairing, anti-inflammatory, antioxidant and antibacterial effects, and is suitable for the treatment of various skin problems such as wounds, burns, acne and postoperative care.

[0084] The preparation method of the medical dressing containing collagen provided by the present invention ensures the uniform mixing and complete dissolution of each component by precisely controlling the temperature, stirring time and filtering steps, strictly controls the temperature during heating and cooling to avoid component denaturation and failure, ensures that all components are fully mixed, avoids stratification and precipitation, and uses 300 mesh filter cloth to filter to remove undissolved particles and other impurities, thereby ensuring the purity of the final product. Gamma ray irradiation sterilization is adopted, which is an efficient and residue-free sterilization method that can completely kill bacteria, viruses and other microorganisms, ensure the sterility of the medical dressing, and avoid infection risks. Strict preparation and sterilization processes ensure the safety and effectiveness of the medical dressing, making it more reliable in clinical applications and suitable for various medical scenarios requiring a sterile environment.

[0085] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A medical dressing containing collagen, characterized in that: The invention is composed of the following raw materials in percentage by weight: 1% to 12% collagen, 1% to 3% biocompatible matrix, 2% to 5% ceramide, 1% to 3% hyaluronic acid, 0.5% to 6% plant extract, 0.1% to 1% carbomer, 1% to 3% glycerol, 2% to 5% propylene glycol, and the balance is purified water.

2. The medical dressing containing collagen according to claim 1, characterized in that: The biocompatible substrate is selected from one or a combination of polylactic acid and chitosan.

3. The medical dressing containing collagen according to claim 1, characterized in that: The collagen is selected from high-purity natural collagen after multi-step pretreatment, and the specific pretreatment steps are: A: Use ultra-fine grinding equipment to refine high-purity natural collagen particles and increase their surface area to obtain natural collagen powder; B: Use protease to partially hydrolyze the natural collagen powder to generate smaller peptides, filter and centrifuge, and take the supernatant for later use to obtain a natural collagen solution; C: Add an appropriate amount of surfactant to the collagen solution to reduce surface tension and improve the dispersibility and stability of collagen.

4. The medical dressing containing collagen according to claim 3, characterized in that: In the step B, the protease is selected from any one of papain and trypsin.

5. The medical dressing containing collagen according to claim 3, characterized in that: In the step C, the surfactant is selected from Tween-80.

6. The medical dressing containing collagen according to claim 3, characterized in that: The natural collagen is selected from any one of fish collagen and marine organism collagen.

7. The medical dressing containing collagen according to claim 1, characterized in that: The plant extract is selected from green tea extract, liquorice extract, Centella asiatica extract and ginkgo leaf extract.

8. The medical dressing containing collagen according to claim 7, characterized in that: The mass ratio of the green tea extract, the liquorice extract, the Centella asiatica extract and the ginkgo leaf extract is 1:3:2:

1.

9. A method for preparing a medical dressing containing collagen according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1: Weigh a required amount of purified water, pour it into a stirring container, slowly add the uniformly mixed biocompatible substrate, carbomer, glycerin and propylene glycol into the purified water while stirring, place the stirring container on a heating device, gradually heat it to 80-85° C., and continue stirring until the biocompatible substrate and carbomer are completely dissolved, to obtain a first mixed solution; S2: Cool the first mixed solution to 40-45° C., slowly add collagen, ceramide, hyaluronic acid and plant extract to the first mixed solution, and continue stirring to ensure that all ingredients are fully mixed, then add the remaining amount of purified water to the mixed solution, and continue stirring until all ingredients are completely dissolved to obtain a uniform second mixed solution; S3: Filter the second mixed liquid through a 300-mesh filter cloth to remove undissolved particles and other impurities to ensure the purity of the mixed liquid; S4: Filling the filtered second mixed liquid into a sterile container and sterilizing the filled medical dressing.

10. The method for preparing a medical dressing containing collagen according to claim 9, characterized in that: The sterilization treatment is carried out by gamma ray irradiation sterilization.

Images