Method for treating aquaculture wastewater by using composite bacteria

Abstract

The present invention discloses a method for treating aquaculture wastewater by using a composite bacterium, which includes putting a composite bacterium agent into the aquaculture wastewater and stirring for treatment; wherein, the preparation method of the composite bacterium agent includes inoculating Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella in a liquid medium respectively to obtain bacterial solutions of Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella; adding porous carbon and the bacterial solutions into a new liquid medium and loading to obtain the composite bacterium agent. When the present invention is used for treatment, it can prevent the loss of enzymes and antibacterial peptides produced by microorganisms, thereby improving the removal rate of ammonia nitrogen in aquaculture wastewater. During the degradation process, part of the nickel is released, improving the activity of microorganisms; the mixing of DL-methionine and the carbon precursor enables good dispersion of nickel species, also increases the specific surface area of the porous carbon, increases the contents of nitrogen element and sulfur element, enhances the interaction between the porous carbon and microorganisms, and significantly improves the removal rate of ammonia nitrogen.

Description

Technical Field

[0001] The present invention relates to the technical field of wastewater treatment by microorganisms, and particularly to a method for treating aquaculture wastewater using a composite bacterium. Background Art

[0002] With the increasing economic development and urbanization level, while bringing convenience to human life, it also brings some disadvantages. At present, the problem of water pollution is becoming increasingly serious with the development of the economy. Environmental pollution problems such as red tides caused by the ineffective treatment of discharged sewage also seriously endanger people's daily lives. Reasonably and effectively solving water pollution can not only effectively alleviate environmental pollution problems, but also, to a certain extent, solve the current problem of water resource shortage. Existing sewage treatment mainly relies on sewage treatment facilities such as sewage treatment plants. Seeking a suitable method to improve the treatment effect of sewage treatment plants is one of the most economical and effective ways to solve water pollution.

[0003] The immobilized microorganism method is developed from the immobilized enzyme method. It is a biological method that immobilizes free microorganisms on a certain carrier, confines them to a specific space, and the microorganisms grow and maintain their activity relying on the carrier and rapidly multiply in large quantities under suitable conditions. Compared with traditional sewage treatment technologies, the immobilized microorganism technology can separate the immobilized microorganisms from the sewage, enabling the microorganisms to maintain a certain concentration and activity and can be reused multiple times. CN114317516A discloses a composite bacterium agent for wastewater treatment and its preparation method. The composite bacterium agent includes Shewanella putrefaciens, Pseudomonas pseudoalcaligenes, and modified zeolite. By introducing chitosan onto the zeolite, the adsorption performance of the zeolite is improved, and then composite modification is carried out to introduce iron phosphate onto the zeolite. Iron ions can be used as a coagulant and cooperate with chitosan to flocculate and sediment ammonia nitrogen substances in the sewage, greatly improving the biological nitrification performance of the composite bacterium agent. However, the preparation method of the modified zeolite in this bacterium agent requires acid treatment, calcination, chitosan crosslinking, and iron ion adsorption, with long and complex steps. CN115838717A discloses a white rot fungus-biochar composite bacterium ball and its preparation method and application. After preparing biochar, nutrient solution, white rot fungus bacterial suspension, and sodium alginate aqueous solution are added, and the obtained mixed solution is oscillated and freeze-dried to obtain the white rot fungus-biochar composite bacterium ball. This method has the advantages of simple process, easy recovery of the bacterium ball, and high treatment ability for heavy metals. However, this bacterium ball mainly uses the adsorption effect, and additional treatment is required after collecting the bacterium ball.

[0004] It is still necessary to provide a composite bacterium with a simple preparation method, strong treatment ability, and not easy to lose. Summary of the Invention

[0005] In order to solve the problems existing in the prior art, the present invention provides a method for treating aquaculture wastewater using a composite bacterium, including,

[0006] Put the compound bacterium agent into the aquaculture wastewater and stir for treatment;

[0007] Among them, the preparation method of the compound bacterium agent includes,

[0008] Inoculate Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella pneumoniae into a liquid medium respectively to obtain the bacterial solutions of Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella pneumoniae;

[0009] Add porous carbon and the bacterial solutions of Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella pneumoniae into a new liquid medium, and load to obtain the compound bacterium agent.

[0010] Compared with single strains, the combined action of multiple microorganisms can often achieve more ideal effects. The microorganisms selected in the present invention are Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella pneumoniae. Enterococcus faecalis is a facultative anaerobic Gram-positive lactic acid bacterium, with a spherical or chain-like cell morphology, a certain diameter, no capsule, no spore, strong adaptability and resistance to the environment, and not strict requirements for growth conditions; Pseudomonas pseudoalcaligenes is a Gram-negative bacterium, motile with a single polar flagellum, colonies are circular, with neat edges, raised, smooth and moist on the surface, and its optimal growth temperature is 35°C, obligate aerobic; Klebsiella pneumoniae is a Gram-negative bacterium, a relatively short and thick bacillus, arranged singly, in pairs or in short chains, without spores, without flagella, with a relatively thick capsule, and most have fimbriae.

[0011] The material used for the immobilized microorganisms in the present invention is nickel-doped porous carbon prepared from biomass, with stable physical and chemical properties. Microorganisms can be well immobilized on it, preventing the loss of enzymes and antibacterial peptides produced by microorganisms during treatment, thereby increasing the degradation rate of ammonia nitrogen in aquaculture wastewater. During the degradation process, part of the nickel is released, improving the activity of microorganisms; the mixing of DL-methionine and the carbon precursor enables good dispersion of nickel species, also increases the specific surface area of the porous carbon, increases the content of nitrogen and sulfur elements, and enhances the interaction between the porous carbon and microorganisms.

[0012] Furthermore, the preparation method of the porous carbon includes,

[0013] Soak the biomass in a nickel salt solution for adsorption, and then collect the insoluble matter;

[0014] Mix the insoluble matter with DL-methionine and calcine it under an inert gas atmosphere to obtain the porous carbon.

[0015] In the present invention, the type of biomass does not need to be strictly limited, and it can be straw, bamboo, bamboo leaves, tree leaves, etc. After collecting the biomass, it needs to be washed, dried, crushed, and passed through a 300-mesh sieve.

[0016] Further, the mass-volume ratio of the biomass to the nickel salt solution is 10-20 g: 100-150 mL;

[0017] The mass ratio of the insoluble matter to DL-methionine is 5-15: 1-3.

[0018] Further preferably, when the insoluble matter is mixed with DL-methionine, di-o-tolylthiourea with a mass 0.05-0.3 times that of the insoluble matter is also added.

[0019] Further, the concentration of the nickel salt solution is 0.05-0.1 mol / L.

[0020] Further, the viable count in the bacterial solutions of Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella is greater than 1.0×10 8 cfu / mL.

[0021] Further, the mass-volume ratio of the porous carbon to the new liquid medium is 0.2-1 g: 1 L.

[0022] Further, the volume ratio of the bacterial solutions of Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella to the new liquid medium is 2-5: 3-10: 2-5: 2000-5000.

[0023] Further, the liquid medium is LB medium. The components of the LB medium are: 10 g of peptone, 5 g of yeast extract, 5 g of NaCl, and 1 L of distilled water.

[0024] Further, the composite bactericide is put into the aquaculture wastewater at a concentration of 1-10 g / L.

[0025] Further, the stirring treatment is carried out at a temperature of 25-35 °C and a rotation speed of 150-300 rpm for 3-7 days.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] In the present invention, Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella are used as active bacteria and immobilized using nickel-doped porous carbon. During the treatment, it can prevent the loss of enzymes and antibacterial peptides produced by microorganisms, thereby improving the removal rate of ammonia nitrogen in aquaculture wastewater. During the degradation process, part of the nickel is released, improving the activity of microorganisms; the mixing of DL-methionine and the carbon precursor enables good dispersion of nickel species, also increases the specific surface area of the porous carbon, increases the content of nitrogen and sulfur elements, enhances the interaction between the porous carbon and microorganisms, and significantly improves the removal rate of ammonia nitrogen. Description of the Drawings

[0028] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0029] Figure 1 A flow chart showing the method for treating aquaculture wastewater using composite bacteria according to the present invention is shown;

[0030] Figure 2 shows a scanning electron microscope image of the porous carbon prepared in Example 1;

[0031] Figure 3 shows a scanning electron microscope image of the porous carbon prepared in Example 2;

[0032] Figure 4 shows a scanning electron microscope image of the porous carbon prepared in Example 3;

[0033] Figure 5 shows a scanning electron microscope image of the porous carbon prepared in Example 4;

[0034] Figure 6 The scanning electron microscope image of the porous carbon prepared in Comparative Example 3 is shown. DETAILED DESCRIPTION

[0035] The endpoints of the ranges and any values disclosed herein are not limited to the precise ranges or values, and these ranges or values should be understood to include values close to these ranges or values. For numerical ranges, the endpoints of each range, the endpoints of each range and individual point values, and the individual point values can be combined with each other to form one or more new numerical ranges, and these numerical ranges should be considered to be specifically disclosed in the present invention.

[0036] Some of the raw materials used in the examples and comparative examples of the present invention are described as follows:

[0037] Enterococcus faecalis, strain number CICC 25019, and Klebsiella pneumoniae, strain number CICC 10870, were purchased from the China Industrial Microbiological Culture Collection Center; Pseudomonas pseudoalcaligenes, strain number CGMCC 1.10611, was purchased from the China General Microbiological Culture Collection Center.

[0038] The biomass was waste bamboo scraps collected from a timber factory. The waste bamboo scraps were washed with water and dried in a constant temperature oven at 120°C for 10 h. They were then crushed with a grinder and passed through a 300-mesh sieve to obtain 300-mesh biomass powder.

[0039] All other raw materials not mentioned are common raw materials in this field. The above content is only for helping to explain the present invention and should not be construed as a strict limitation of the present invention. Those skilled in the art can directly purchase from the market or prepare the same / similar raw materials by themselves. These contents will not be elaborated in the examples anymore.

[0040] Next, specific embodiments of the present invention will be combined to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of the present invention.

[0041] Example 1

[0042] As Figure 1 shown, a method for treating aquaculture wastewater using composite bacteria is as follows.

[0043] S1. Prepare LB medium according to the ratio of 10 g of peptone, 5 g of yeast extract leaching solution, 5 g of NaCl, and 1 L of distilled water.

[0044] S2. Inoculate Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella pneumoniae into the LB medium respectively according to the inoculation amount of 10% by volume ratio, transfer to a shaker, and culture at a temperature of 25°C and a shaking speed of 150 rpm to obtain bacterial solutions of Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella pneumoniae with viable cell counts of 1.0×10 9 cfu / mL respectively.

[0045] S3. Add 3 g of porous carbon, 5 mL of the bacterial solution of Enterococcus faecalis, 10 mL of the bacterial solution of Pseudomonas pseudoalcaligenes, and 5 mL of the bacterial solution of Klebsiella pneumoniae to 5 L of fresh LB medium, transfer to a shaker, and culture at a temperature of 30°C and a shaking speed of 150 rpm for 24 h for sufficient loading, and then freeze-dry to obtain a composite bactericide.

[0046] S4. Put the composite bactericide into the simulated aquaculture wastewater at a concentration of 6 g / L and continuously stir at a temperature of 32°C and a rotation speed of 220 rpm for 5 days. The simulated aquaculture wastewater contains 260 mg / L of ammonia nitrogen, 15 mg / L of nitrate nitrogen, and 8 mg / L of total phosphorus.

[0047] Among them, the preparation method of the porous carbon is as follows.

[0048] T1. Immerse 15 g of biomass in 120 mL of an aqueous solution of nickel chloride hexahydrate with a concentration of 0.05 mol / L, keep it for 12 h for adsorption, filter, and then place it in a constant temperature oven at 120°C for drying for 10 h to obtain a carbon precursor.

[0049] T2. After mixing 80 g of carbon precursor with 24 g of DL-methionine, transfer the mixture to a tube furnace under a nitrogen atmosphere, heat it to 700 °C at a heating rate of 3 °C / min, hold for 1.5 h, and then cool it naturally. Wash the black powder three times with water and ethanol respectively, filter it, and transfer it to a constant temperature oven at 120 °C to dry for 10 h to obtain porous carbon.

[0050] Example 2

[0051] A method for treating aquaculture wastewater using composite bacteria is basically the same as that in Example 1, except that in step T1 of the preparation method of porous carbon, the biomass is immersed in an aqueous solution of nickel chloride hexahydrate with a concentration of 0.075 mol / L.

[0052] Example 3

[0053] A method for treating aquaculture wastewater using composite bacteria is basically the same as that in Example 1, except that in step T1 of the preparation method of porous carbon, the biomass is immersed in an aqueous solution of nickel chloride hexahydrate with a concentration of 0.1 mol / L.

[0054] Example 4

[0055] A method for treating aquaculture wastewater using composite bacteria is as follows.

[0056] S1. Prepare LB medium according to the ratio of 10 g of peptone, 5 g of yeast extract leachate, 5 g of NaCl, and 1 L of distilled water.

[0057] S2. Inoculate Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella pneumoniae into LB medium according to an inoculation amount of 10% by volume ratio, transfer it to a shaker, and culture it at a temperature of 25 °C and a shaking speed of 150 rpm to obtain bacterial solutions of Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella pneumoniae with viable cell counts of 1.0×10 9 cfu / mL respectively.

[0058] S3. Add 3 g of porous carbon, 5 mL of the bacterial solution of Enterococcus faecalis, 10 mL of the bacterial solution of Pseudomonas pseudoalcaligenes, and 5 mL of the bacterial solution of Klebsiella pneumoniae to a new 5 L LB medium, transfer it to a shaker, and culture it at a temperature of 30 °C and a shaking speed of 150 rpm for 24 h for sufficient loading, and then freeze-dry to obtain a composite bactericide.

[0059] S4. Put the composite bactericide into the simulated aquaculture wastewater at a concentration of 6 g / L and continue for 5 days at a temperature of 32 °C and a rotation speed of 220 rpm. The simulated aquaculture wastewater contains 260 mg / L of ammonia nitrogen, 15 mg / L of nitrate nitrogen, and 8 mg / L of total phosphorus.

[0060] Among them, the preparation method of the porous carbon is as follows.

[0061] T1. Immerse 15 g of biomass in 120 mL of an aqueous solution of nickel chloride hexahydrate at 0.075 mol / L, maintain for 12 h for adsorption, and after filtration, place it in a constant-temperature oven at 120 °C and dry for 10 h to obtain a carbon precursor;

[0062] T2. Mix 80 g of the carbon precursor, 15 g of DL-methionine, and 8 g of di-o-tolylthiourea, transfer them to a tubular furnace under a nitrogen atmosphere, heat at a heating rate of 3 °C / min to 700 °C, hold for 1.5 h, and after natural cooling, wash the black powder three times with water and ethanol respectively, and after filtration, transfer it to a constant-temperature oven at 120 °C and dry for 10 h to obtain porous carbon.

[0063] Comparative Example 1

[0064] A method for treating aquaculture wastewater using composite bacteria is basically the same as that in Example 1, except that in step T1 of the preparation method of porous carbon, the biomass is immersed in an aqueous solution of nickel chloride hexahydrate at 0.15 mol / L.

[0065] Comparative Example 2

[0066] A method for treating aquaculture wastewater using composite bacteria is basically the same as that in Example 2, except that in step T2 of the preparation method of porous carbon, DL-methionine is not introduced.

[0067] Comparative Example 3

[0068] A method for treating aquaculture wastewater using composite bacteria is as follows:

[0069] S1. Prepare an LB medium according to the ratio of 10 g of peptone, 5 g of yeast extract leachate, 5 g of NaCl, and 1 L of distilled water;

[0070] S2. Inoculate Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella pneumoniae into the LB medium respectively according to an inoculation amount of 10% by volume ratio, transfer to a shaker, and culture at a temperature of 25 °C and a shaking speed of 150 rpm to obtain bacterial solutions of Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella pneumoniae with viable cell counts of 1.0×10 9 cfu / mL each;

[0071] S3. Add 3 g of porous carbon, 5 mL of the bacterial solution of Enterococcus faecalis, 10 mL of the bacterial solution of Pseudomonas pseudoalcaligenes, and 5 mL of the bacterial solution of Klebsiella pneumoniae to 5 L of a new LB medium, transfer to a shaker, and culture at a temperature of 30 °C and a shaking speed of 150 rpm for 24 h for sufficient loading, and then freeze-dry to obtain a composite bacterial agent;

[0072] S4. The composite bacterium agent was put into the simulated aquaculture wastewater at a concentration of 6 g / L and continuously treated for 5 days at a temperature of 32°C and a rotation speed of 220 rpm. The simulated aquaculture wastewater contained 260 mg / L of ammonia nitrogen, 15 mg / L of nitrate nitrogen, and 8 mg / L of total phosphorus.

[0073] Among them, the preparation method of the porous carbon is as follows.

[0074] 80 g of biomass and 24 g of DL-methionine were mixed and then transferred to a tube furnace under a nitrogen atmosphere. The temperature was raised to 700°C at a heating rate of 3°C / min and then held for 1.5 h. After natural cooling, the black powder was washed three times with water and ethanol respectively, and then filtered and transferred to a constant temperature oven at 120°C for drying for 10 h to obtain the porous carbon.

[0075] Comparative Example 4

[0076] It was basically the same as Example 4, except that: in step T2 of the preparation method of the porous carbon, 80 g of carbon precursor and 24 g of di-o-tolylthiourea were mixed and then calcined.

[0077] Test Example

[0078] The scanning electron microscope was used to observe the microscopic morphology of the porous carbon materials of Examples 1 to 4 and Comparative Example 3, and the results are as Figures 2 to 6 shown. It can be seen from Figure 6 that the porous carbon prepared without soaking in nickel salt solution presents a lamellar structure with a large number of wrinkles on the surface, and such a structure is beneficial to the enrichment of microorganisms on its surface. It can be seen from Figure 2 and Figure 4 that larger particles of nickel species appear on the porous carbon whether the concentration of the nickel chloride aqueous solution is high or low. It can be seen from Figure 3 that an appropriate nickel concentration can control the size of the nickel species, Figures 2 to 4 and the nickel species are concentrated at the wrinkles. In Example 4 where di-o-tolylthiourea was added while adding DL-methionine, Figure 5 as shown, the nickel species are well dispersed on the surface of the porous carbon without obvious agglomeration, and such a structure may contribute to the interaction with microorganisms.

[0079] The specific surface area of the porous carbon was tested by nitrogen adsorption and desorption, and the results are shown in Table 1.

[0080] Table 1 Specific surface area of porous carbon

[0081]

[0082]

[0083] As can be seen from the test results in Table 1, the specific surface area of the porous carbon in Comparative Example 3 is the lowest. It is obtained by calcining the mixture of biomass and DL-methionine. In other examples, nickel salt soaking is carried out. The nickel salt will decompose during calcination, etching the formed carbon and thus increasing the pore size. The nickel salt concentration gradually increases from Example 1 to Example 3, and the specific surface area decreases in turn. This is because the generated nickel species block the pore size. Compared with Example 2, DL-methionine is not added in Comparative Example 2, and the specific surface area also decreases significantly, indicating that the addition of DL-methionine enhances the etching effect. And Example 4 has the largest specific surface area, which shows that DL-methionine and di-o-tolylthiourea enhance the dispersion of nickel species on the surface of the porous carbon, thus further increasing the specific surface area.

[0084] The removal rate results of pollutants in the simulated aquaculture wastewater for the examples and comparative examples are shown in Table 2.

[0085] Table 2 Removal rate results of pollutants in the simulated aquaculture wastewater

[0086]

[0087] As can be seen from the test results in Table 2, the examples of the present invention have good removal ability for ammonia nitrogen and nitrate nitrogen. Except that Example 4 has good removal of phosphorus, the removal effects of other examples are not ideal. Among the nickel-containing groups, the removal rate of Comparative Example 1 is relatively poor. This may be because the higher nickel content results in a larger amount of dissolved nickel ions, which instead inhibits the activity of microorganisms. This is because the larger specific surface area and good dispersion of nickel species enhance the interaction between the porous carbon and microorganisms, making the decomposition enzymes and antibacterial peptides produced by microorganisms not easily lost. During the degradation process, part of the nickel is released, improving the activity of microorganisms. Therefore, Example 4 has good removal rates for ammonia nitrogen, nitrate nitrogen and phosphorus.

[0088] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for treating aquaculture wastewater using composite bacteria, characterized in that, Including, Put the compound bacterial agent into the aquaculture wastewater and stir for treatment; Among them, the preparation method of the compound bacterial agent includes, Inoculate Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella pneumoniae in a liquid medium respectively to obtain the bacterial solutions of Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella pneumoniae; Add porous carbon and the bacterial solutions of Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella pneumoniae to a new liquid medium, and load to obtain the compound bacterial agent; The preparation method of the porous carbon includes, Soak the biomass in a nickel salt solution for adsorption, and then collect the insoluble matter; Mix the insoluble matter with DL-methionine and calcine it in an inert gas atmosphere to obtain porous carbon; among them, when mixing the insoluble matter with DL-methionine, add 0.05 - 0.3 times the mass of the insoluble matter of di-o-tolylthiourea; The mass ratio of the insoluble matter to DL-methionine is 5 - 15:1 - 3; The mass-to-volume ratio of the biomass to the nickel salt solution is 10 - 20 g:100 - 150 mL; The concentration of the nickel salt solution is 0.05 - 0.1 mol / L.

2. The method for treating aquaculture wastewater using composite bacteria according to claim 1, characterized in that The viable cell counts in the bacterial suspensions of Enterococcus faecalis, Pseudomonas pseudoalcaligenes and Klebsiella are all greater than 1.0×10 8 cfu / mL.

3. The method for treating aquaculture wastewater using composite bacteria according to claim 1, characterized in that, The mass-to-volume ratio of the porous carbon to the new liquid medium is 0.2 - 1 g:1 L.

4. The method for treating aquaculture wastewater using compound bacteria according to claim 1, characterized in that, The volume ratio of the bacterial solutions of Enterococcus faecalis, Pseudomonas pseudoalcaligenes, and Klebsiella pneumoniae to the new liquid medium is 2 - 5:3 - 10:2 - 5:2000 - 5000.

5. The method for treating aquaculture wastewater by using composite bacteria according to claim 1, characterized in that, The liquid medium is LB medium.

6. The method for treating aquaculture wastewater using composite bacteria according to claim 1, wherein The compound bacterial agent is put into the aquaculture wastewater at a concentration of 1 - 10 g / L.

7. The method for treating aquaculture wastewater using composite bacteria according to claim 1, characterized in that, The stirring treatment is carried out at a temperature of 25 - 35 °C and a rotation speed of 150 - 300 rpm for 3 - 7 days.

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