Anaerobic ammonia oxidation strain cultivation device and use method

Abstract

The invention relates to an anaerobic ammonium oxidation strain cultivation device and a use method, the device comprises a cultivation tank structure module, an incubation bed module, a regulation and control module and a heat recovery module, the cultivation tank structure module comprises a water inlet mechanism, a cultivation tank main body mechanism, an internal circulation mechanism and a water outlet mechanism, and the water inlet mechanism and the water outlet mechanism are located on the two sides of the internal circulation mechanism; the incubation bed module is positioned in the cultivation tank main body, comprises an incubation tube, graphite and fiber net flowers, and is used for inoculating initial strains of anaerobic ammonium oxidation bacteria; the regulation and control module is located on one side of the cultivation tank and used for controlling parameters of water inlet and outlet flow and hydraulic retention time of the device; the heat recovery module is arranged in the cultivation tank and beside the cultivation tank, comprises a turbine, a piston cylinder, a heat exchange pipe and a heat exchanger, and is used for absorbing heat in water discharged from the device and releasing heat in the water inlet mechanism and the incubation pipe. The anaerobic ammonium oxidation bacteria in various different application scenes can be cultivated, the growth efficiency of the flocculent anaerobic ammonium oxidation bacteria is improved, and the cultivation energy consumption is greatly reduced.

Description

Technical Field

[0001] The present invention belongs to the field of bacterial strain cultivation, and more specifically, relates to an anaerobic ammonia oxidation bacterial strain cultivation device and a use method thereof. Background Art

[0002] my country's current industrial development is rapid, and the accompanying environmental pollution is becoming increasingly serious. It is difficult to achieve low-cost and effective treatment of water bodies such as leachate and livestock wastewater. The cost of denitrification of domestic sewage in many areas is also high. This is because the traditional biochemical treatment method, although not recognized by the industry as a low-cost and high-efficiency treatment method, requires a large amount of oxygen and carbon source in the denitrification process due to the metabolic characteristics of traditional microorganisms themselves, thus generating a large amount of electricity and energy consumption, and also producing large carbon emissions. It is not an environmentally friendly and energy-saving water treatment technology.

[0003] In recent years, the new autotrophic denitrification technology based on anaerobic ammonia oxidation has been gradually promoted. Since this technology is autotrophic denitrification, it does not require carbon source consumption and can greatly reduce the aeration energy consumption required for the overall denitrification process. Therefore, some water plants have gradually used it as the core technology of the denitrification section. However, there are fewer anaerobic ammonia oxidizing bacteria, slow growth rate, strong temperature dependence, and easy loss. Therefore, it is more complicated to cultivate. At present, most of the breeding methods are to use traditional UASB or similar reactors for breeding. By adding a certain proportion of ammonia nitrogen and nitrite nitrogen to the influent, and using electric heating to raise the temperature in the reactor to 32-35°C, and then breeding in batch water inlet mode. The breeding efficiency of this method is poor. When cultivating anaerobic ammonia oxidizing bacteria on a large scale, heating will generate a lot of energy consumption. When cultivating flocculent anaerobic ammonia oxidizing bacteria, a large amount of sludge will be lost due to the floating of bacteria. When cultivating granular anaerobic ammonia oxidizing bacteria, some low-density particles will also be lost, making it difficult to achieve large-scale effective expansion.

[0004] Therefore, there is an urgent need to develop a new set of efficient breeding equipment to significantly reduce the cost of bacterial cultivation, reduce electricity consumption and costs during the cultivation process, and realize the green, large-scale and rapid cultivation of anaerobic ammonia-oxidizing bacteria. Summary of the invention

[0005] In order to solve the deficiencies in the prior art, the present invention combines the growth characteristics of anaerobic ammonium oxidizing bacteria and the application characteristics of anaerobic ammonium oxidizing bacteria in engineering to provide an anaerobic ammonium oxidizing bacteria cultivation device and method that can significantly reduce the cultivation cost of bacteria and realize large-scale rapid cultivation of anaerobic ammonium oxidizing bacteria.

[0006] The present invention adopts the following technical solution.

[0007] The first aspect of the present invention discloses an anaerobic ammonia-oxidizing bacteria cultivation device, comprising: a cultivation tank structure module, an incubation bed module, a control module and a heat recovery module, wherein the cultivation tank structure module comprises: a water inlet mechanism, a cultivation tank main body mechanism, an internal circulation mechanism and a water outlet mechanism, wherein the water inlet mechanism and the water outlet mechanism are located on both sides of the internal circulation mechanism; a viewing window is also provided on the cultivation tank main body structure for observing the internal state of the cultivation tank;

[0008] The incubation bed module is located inside the main body of the cultivation tank and includes: an incubation tube, graphite and a fiber mesh flower, and is used to inoculate the initial strain of anaerobic ammonia-oxidizing bacteria;

[0009] The control module is located on one side of the cultivation tank and is used to control the parameters of the inlet and outlet water flow and hydraulic retention time of the device;

[0010] The heat recovery module is arranged inside the cultivation tank and beside the cultivation tank, and includes: an impeller, a piston cylinder, a heat exchange tube and a heat exchanger, which is used to absorb the heat in the water outlet of the device and release the heat in the water inlet mechanism and the incubation tube.

[0011] Preferably, the water inlet mechanism is composed of a water pump and a water inlet tray, the water inlet tray is located at a height of 20-35% from the bottom to the top of the cultivation tank, and the tray surface is composed of a water retainer and a water distribution ring.

[0012] Preferably, the water retainer can be switched to manual control or an external control mechanism to adjust the shielding position and shielding ratio.

[0013] Preferably, the culture tank structure consists of an inner tube and an outer tube, the inner diameter difference between the inner tube and the outer tube is 10 to 500 mm, all probes, electronic control circuits, and water inlet and outlet pipes of the device are laid to the bottom of the device through the interlayer, and finally extend to the outside of the device, and insulation material is laid between the interlayers.

[0014] Preferably, the internal circulation mechanism is composed of an internal circulation water inlet pump and a U-shaped annular tube. The annular tube is arranged upright in the main body or at an angle of 0 to 30° to the vertical direction. The inner diameter of the annular tube is 100 to 500 mm, and the total height is 30 to 70% of the height of the tank body. Within the elliptical area, a small hole is opened every 10 to 100 mm, and the aperture of the small hole is 10 to 30 mm. The water inlet and outlet of the U-shaped annular tube are 2 to 8 cm away from the top liquid level.

[0015] Preferably, the heat recovery module collects the gas generated during the incubation process of the incubation bed module into a collector through the exhaust gas collection port. One end of the collector is immersed in the reactor, and the other end is inside the water outlet pipe. The gas in the collector is compressed by a reciprocating compressor, and then the pressure is released through a pressure relief valve. The released gas is mixed with the newly generated gas and enters the collector again.

[0016] Preferably, the water outlet mechanism includes a second water inlet, an internal reflux port, an overflow port, a water outlet and a flow path. The water in the reactor first flows into the water outlet through the water inlet, and first reaches the internal reflux port through the internal path. The internal reflux port is connected to the water inlet end of the internal circulation structure, and the water in this area is sucked into the internal circulation structure. The water in the reactor will pass through the overflow port directly to the water outlet.

[0017] Preferably, the incubation tube comprises several turns of coils, one end of which is placed at the bottom of the device on one side of the water inlet tray, and the coils are tied with fluff containing 20-40% graphite ribbon and 60-80% fiber filaments every 50-300 mm. Cams are provided at the top and bottom of the coils, and a water outlet mechanism is provided at the bottom.

[0018] Preferably, the control module includes any one of an online DO probe, an online ammonia nitrogen probe, an online nitrite probe, an online nitrate probe, an online pH probe, an online pressure gauge, and an online liquid level gauge placed in the reactor.

[0019] The second aspect of the present invention discloses a method for cultivating anaerobic ammonium-oxidizing bacteria. The method for cultivating anaerobic ammonium-oxidizing bacteria comprises the following steps:

[0020] Step 1, adding activated sludge or anaerobic ammonium oxidation seed sludge with a MLSS concentration of 10 to 1000 mg / L into the reactor, and then letting the culture medium enter the main body of the cultivation tank through the water inlet mechanism of the device under the parameters set by the pre-configured culture medium;

[0021] Step 2, start the control module, and control the hydraulic retention time of the outlet water according to the changes in the concentrations of ammonia nitrogen, nitrite nitrogen, and nitrate nitrogen in the inlet and outlet water to ensure that the concentration difference of ammonia nitrogen and nitrite nitrogen in the inlet and outlet water is above 50 mg / L;

[0022] Step 3, regularly start the water pump at the suction end of the water outlet mechanism to ensure smooth overflow of water;

[0023] Step 4, start the heat recovery device to recover the outlet water heat;

[0024] Step 5: When the MLSS concentration inside the device is higher than 5000 mg / L, more than half of the internal sludge is discharged, and then the process of steps 2 to 4 is repeated to continue the cultivation of anaerobic ammonia-oxidizing bacteria.

[0025] The beneficial effect of the present invention is that, compared with the prior art,

[0026] 1) The device of the present invention can cultivate anaerobic ammonium oxidizing bacteria for various application scenarios, such as flocculent anaerobic ammonium oxidizing bacteria, granular anaerobic ammonium oxidizing bacteria, and can also cultivate anaerobic ammonium oxidizing bacteria growing on biofilms.

[0027] 2) The unique water inlet mechanism designed in the device of the present invention can effectively change the flow state of the water inside the reactor. On the one hand, it can make the internal substrate matrix evenly mixed. On the other hand, it can prevent the problem of large-scale floating of bacteria when cultivating flocculent anaerobic ammonia-oxidizing bacteria, thereby greatly improving the growth efficiency of flocculent anaerobic ammonia-oxidizing bacteria.

[0028] 3) The unique water outlet mechanism designed in the device of the present invention can effectively intercept the anaerobic ammonia-oxidizing bacteria floating or scattered in the water, so that 99% of the anaerobic ammonia-oxidizing bacteria can be effectively intercepted inside the reactor, preventing the problem of bacterial species loss caused by water outflow; at the same time, the reactor has established a special incubation tube cultivation mode, using a heat pump to recover the heat in the effluent water, and transfer this part of the recovered heat to the inside of the incubation tube, and increase the local temperature inside the reactor through the incubation tube, effectively using the effluent heat to provide a more suitable growth environment for the anaerobic ammonia-oxidizing bacteria inside the reactor, improve its growth efficiency, and greatly reduce the energy consumption of cultivation. In addition, the reactor probe monitoring system can also well detect the real-time status inside the reactor. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a structural diagram of the anaerobic ammonia-oxidizing bacteria cultivation device of the present invention;

[0030] Figure 2 It is an enlarged schematic diagram of the internal circulation structure in the present invention;

[0031] Figure 3 It is a schematic diagram of the water inlet structure in the present invention;

[0032] Figure 4 It is a schematic diagram of the water outlet mechanism in the present invention;

[0033] In the figure: 1. water inlet mechanism; 2. control module; 3. compressed gas inlet pipe; 4. incubation bed module; 5. internal circulation mechanism; 6. internal circulation water inlet pump; 7. window; 8. cultivation tank body; 9. exhaust gas collection port; 10. gas reflux pipeline; 11. cold recovery device; 12. back pressure valve; 13. pressure reducing valve; 14. water outlet pipeline; 15. water outlet mechanism; 16. piston cylinder; 17. power transmission rod; 18. impeller; 19. first water inlet; 20. transmission rod; 21. driving shaft; 22. water retainer; 23. water distribution ring; 24. second water inlet; 25. water outlet; 26. overflow port; 27. internal reflux port. DETAILED DESCRIPTION

[0034] In order to make the purpose, technical scheme and advantages of the present invention clearer, the technical scheme of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. The embodiments described in this application are only part of the embodiments of the present invention, not all of them. Based on the spirit of the present invention, other embodiments obtained by ordinary technicians in this field without creative work are all within the scope of protection of the present invention.

[0035] Embodiment 1 of the present invention provides a novel anaerobic ammonia-oxidizing bacteria cultivation device, comprising a cultivation tank structure module, an incubation bed module, a control module 2 and a heat recovery module.

[0036] The cultivation tank structural module is the cultivation tank main structure 8, which includes a water inlet mechanism 1, an internal circulation mechanism 5 and a water outlet mechanism 15, wherein the water inlet mechanism 1 and the water outlet mechanism 15 are located on both sides of the internal circulation mechanism; a viewing window 7 is also provided on the cultivation tank main structure 8 for observing the internal state of the cultivation tank.

[0037] The incubation bed module 4 is located inside the main body of the cultivation tank and includes an incubation tube, graphite and a fiber mesh flower;

[0038] The control module 2 is located next to the cultivation tank and can control the inlet and outlet water flow of the device, the opening and closing of the main valves, the hydraulic retention time and other parameters;

[0039] The heat recovery module is located inside and beside the cultivation tank, and includes an impeller 18, a piston cylinder 16, a heat exchange tube, a heat exchanger, and the like.

[0040] The impeller 18 is driven by the outlet water or return water or the auxiliary motor, and the generated power is transmitted to the piston cylinder 16 through the power transmission rod 17; the piston cylinder 16 compresses the gas collected by the reactor, and presses the compressed gas into the incubation tube in the incubation bed module through the compressed gas inlet pipe 3. The incubation tube completes heat release inside the incubation tank and then enters the external cold recovery device. The cold recovery device 11 is provided with a back pressure valve 12 and a pressure reducing valve 13. The compressed exhaust gas is depressurized in this area and the cold is recovered at the same time. The depressurized gas at the exhaust gas collection port 9 is merged with the gas collected in the reactor through the gas reflux pipeline 10 and enters the next cycle.

[0041] When cultivating anaerobic ammonia-oxidizing bacteria, the culture medium is introduced into the cultivation tank body through the water inlet mechanism of the device in a continuous or batch manner under the pre-configured culture medium setting parameters, and then discharged through the water outlet mechanism. The initial strain of anaerobic ammonia-oxidizing bacteria is inoculated on the incubation bed module, and the heat of the outlet water is recovered through the heat recovery module.

[0042] like Figure 3As shown, in a preferred but non-limiting embodiment of the present invention, in the culture tank structure module, the water inlet mechanism is composed of a water pump and a water inlet tray, the water inlet tray is located at a height of 20 to 35% from the bottom to the top of the culture tank, and the water inlet tray includes a first water inlet 19, a transmission rod 20, a drive shaft 21, a water retainer 22 and a water distribution ring 23;

[0043] The water retainer 22 and the water distribution ring 23 are connected together to form a water inlet tray. The side of the water retainer 22 that contacts the water distribution ring 23 is provided with an L-shaped or T-shaped groove channel. The incoming water flow can push the water retainer 22 to rotate around the water distribution ring 23 through the impeller to block part or all of the water inlet. In this way, since the water outlet is very close to the incubation tube, an impact water inlet effect will be produced.

[0044] In a further preferred embodiment, the water retainer 22 can be switched to manual control or an external control mechanism to adjust the shielding position and shielding ratio.

[0045] In a preferred but non-limiting embodiment, in the main structure of the culture tank structure, the main structure is composed of an inner tube and an outer tube, and the inner diameter difference between the inner tube and the outer tube is in the range of 10 to 500 mm. All probes, electronic control circuits, and inlet and outlet water pipes of the device are laid to the bottom of the reactor through the interlayer and finally extend to the outside of the reactor. Insulation material is laid between the interlayers to improve the insulation effect of the device.

[0046] Further preferably, the thermal insulation material is any one of aerogel, foamed polyurethane, rock wool and the like.

[0047] When water enters the reactor, the internal liquid level of the reactor will rise, and the water will flow upward from the bottom into the water outlet mechanism, and flow from the lower water inlet along the maze path to the right water outlet. During normal operation, the round hole in the upper left part is connected to the bottom of the reactor through a pipe, and the water in this area is periodically returned to the bottom of the reactor through the pipe. The square opening at the upper end is always open. When the outlet is blocked or the water cannot be discharged in time, this outlet can ensure that the water overflows out of the water outlet mechanism.

[0048] like Figure 2 As shown, in a preferred but non-restrictive embodiment, in the culture tank structure module, the internal circulation mechanism is composed of an internal circulation water inlet pump 6 and a U-shaped annular tube, and the annular tube is erected in the main body or arranged at 0 to 30 degrees to the vertical direction. The inner diameter of the annular tube is 100 to 500 mm, and the total height is 30 to 70% of the height of the tank body. Within the elliptical area, a small hole is opened every 10 to 100 mm, and the aperture of the small hole is 10 to 30 mm. The water inlet and outlet of the U-shaped annular tube are 2 to 8 cm above the top liquid level. The water flow direction of the water inlet is along the direction of gravity (there may be an angle of less than 30 degrees), and the water flow direction of the water outlet is generally opposite to the water flow direction of the water inlet.

[0049] In a preferred but non-limiting embodiment, Figure 4 As shown, in the culture tank structure module, the water outlet mechanism 15 is composed of a second water inlet 24, an internal reflow port 27, an overflow port 26, a water outlet 25 and a special flow path. The water to be discharged in the device enters and exits the water outlet of the water outlet device in advance, and flows out along the structure shown in the figure, and the water inlet and the water outlet form an angle of 15 to 45 degrees. During the entire water discharge process, after the water flows into the water inlet, it needs to pass through at least 5 right-angle bends (or bends greater than 75 degrees) before it can flow out (when there are more than 5 right-angle bends), and one or more internal reflow ports 27 can be set.

[0050] In a further preferred embodiment, the water in the reactor first flows into the water outlet through the water inlet, and first reaches the internal reflux port through the internal path. The internal reflux port is connected to the water inlet end of the internal circulation structure, and the water in this area can be intermittently or continuously pumped into the internal circulation structure. However, since the water outlet mechanism and the internal circulation mechanism are linked, the suction flow rate does not affect the final water outlet flow rate.

[0051] Subsequently, the water in the reactor will pass through the overflow end and directly reach the water outlet. The overflow end can prevent the reactor liquid level from rising due to excessive instantaneous drainage, and can also prevent the blockage of the water inlet and suction end, resulting in water discharge failure.

[0052] In a preferred but non-limiting embodiment, in the incubation bed module, the incubation tube is composed of several circles of coils, one end of the coil is placed directly at the bottom of the device near the water inlet tray, and the coil is tied with fluff containing 20-40% graphite tape and 60-80% fiber filaments every 50-300 mm. A cam with a "," structure is provided on the top or bottom of the coil. The cam is slowly driven by the impeller at the water outlet end, causing the incubation tube to shake periodically and return to its original position instantly to prevent regional blockage.

[0053] In a preferred but non-limiting embodiment, the control module includes an online DO probe, an online ammonia nitrogen probe, an online nitrite probe, an online nitrate probe, an online pH probe, an online pressure gauge, an online liquid level gauge, etc. placed in the reactor, which can detect the corresponding data inside the reactor and adjust parameters such as the inlet and outlet water.

[0054] In a preferred but non-limiting embodiment, in the heat recovery module, the compressor and the pressure relief valve will forcibly absorb the heat in the outlet water of the device by means of a heat pump, and then release the heat in the water inlet tray and the incubation tube.

[0055] Embodiment 2 of the present invention provides a method for cultivating anaerobic ammonium-oxidizing bacteria, comprising the following steps:

[0056] Step 1, adding activated sludge or anaerobic ammonium oxidation seed sludge with a MLSS concentration of 10 to 1000 mg / L into the reactor, and then continuously or batch-wise introducing the culture medium into the main body of the cultivation tank through the water inlet mechanism of the device under the parameters set by the pre-configured culture medium;

[0057] Step 2, start the control module, and control the hydraulic retention time of the outlet water according to the changes in the concentrations of ammonia nitrogen, nitrite nitrogen, and nitrate nitrogen in the inlet and outlet water to ensure that the concentration difference of ammonia nitrogen and nitrite nitrogen in the inlet and outlet water is above 50 mg / L;

[0058] Step 3, regularly start the water pump at the suction end of the water outlet mechanism to ensure smooth overflow of water;

[0059] Step 4, start the heat recovery device to recover the outlet water heat;

[0060] Step 5: When the MLSS concentration inside the device is higher than 5000 mg / L, more than half of the internal sludge is discharged, and then the process of steps 2 to 4 is repeated to achieve continuous anaerobic ammonia-oxidizing bacteria cultivation.

[0061] Another embodiment of the present invention further illustrates the technical effects of the present invention by applying different cultivation devices, specifically:

[0062] Four groups of reactors of the same volume were started at the same time, with an inner diameter of 540 mm and a height of 1.3 m (effective height 1.0 m). The four groups of reactors were named R1, R2, R3, and R4, respectively. The structures and accessories of the R1 and R2 reactors were built in accordance with this patent, R3 was a traditional upflow reactor, and R4 was a traditional upflow reactor with external water bath heating. The four groups of reactors were operated under the same room temperature environment, and the parameters of inoculated microbial biomass, influent concentration, and hydraulic retention time were consistent.

[0063] Since R1 and R2 are equipped with heat recovery devices and R4 is equipped with water bath heating, the operating temperature of these three reactors is maintained at 30±3℃. The operating temperature of R3 is 23±3℃.

[0064] In the first stage of the reactor operation, the four groups of reactors were inoculated with the same sludge concentration and the same batch of anaerobic ammonium oxidation seed sludge. After inoculation, the sludge concentration in the reactor was 628 mg MLSS / L. During the subsequent 60 days of operation, the influent ammonia nitrogen concentration was 100 mg / L and the influent nitrite concentration was 130 mg / L.

[0065] During the first 30 days of operation, the total nitrogen load of the influent to the four reactors was 0.46 kg N /

[0066] (m 3·d), after 30 days, the sludge concentration of reactor R1 reached 2689mg MLSS / L, the sludge concentration of reactor R2 reached 2561mg MLSS / L, the sludge concentration of reactor R3 was 973mg MLSS / L, and the sludge concentration of reactor R4 was 1439mg MLSS / L. To maintain 30±3℃, under the same insulation environment, the comprehensive power consumption of reactor R1 was 11.6 degrees, the comprehensive power consumption of reactor R2 was 11.2 degrees, and the comprehensive power consumption of reactor R4 was 56.2 degrees;

[0067] The above data indicate that the reactor constructed by the present invention can well promote the growth of anaerobic ammonia-oxidizing bacteria and can realize rapid cultivation of bacteria under very low energy consumption conditions.

[0068] In the next 30 days, we remixed the sludge from each reactor, diluted it again and inoculated it into reactors R1 to R4. After inoculation, the sludge concentration in the reactor was 731 mg MLSS / L, and the total nitrogen concentration in the effluent of the four reactors was controlled at around 20 mg / L. After 30 days, the sludge concentration in reactor R1 reached 3789 mg MLSS / L, the sludge concentration in reactor R2 reached 3666 mg MLSS / L, the sludge concentration in reactor R3 was 862 mg MLSS / L, and the sludge concentration in reactor R4 was 1524 mg MLSS / L.

[0069] This data trend once again shows that the reactor constructed by the present invention can promote the growth of anaerobic ammonia-oxidizing bacteria at very low energy consumption.

[0070] The beneficial effect of the present invention is that, compared with the prior art,

[0071] 1) The device of the present invention can cultivate anaerobic ammonium oxidizing bacteria for various application scenarios, such as flocculent anaerobic ammonium oxidizing bacteria, granular anaerobic ammonium oxidizing bacteria, and anaerobic ammonium oxidizing bacteria growing on biofilms;

[0072] 2) The unique water inlet mechanism designed in the device of the present invention can effectively change the flow pattern of water inside the reactor, which can make the internal substrate matrix evenly mixed on the one hand, and prevent the problem of large-scale floating of bacteria when cultivating flocculent anaerobic ammonia-oxidizing bacteria on the other hand, thereby greatly improving the growth efficiency of flocculent anaerobic ammonia-oxidizing bacteria;

[0073] 3) The unique water outlet mechanism designed in the device of the present invention can effectively intercept the anaerobic ammonia-oxidizing bacteria floating or scattered in the water, so that 99% of the anaerobic ammonia-oxidizing bacteria can be effectively intercepted inside the reactor, preventing the problem of bacterial species loss caused by water outflow; at the same time, the reactor has established a special incubation tube cultivation mode, using a heat pump to recover the heat in the effluent water, and transfer this part of the recovered heat to the inside of the incubation tube, and increase the local temperature inside the reactor through the incubation tube, effectively using the effluent heat to provide a more suitable growth environment for the anaerobic ammonia-oxidizing bacteria inside the reactor, improve its growth efficiency, and greatly reduce the energy consumption of cultivation. In addition, the reactor probe monitoring system can also well detect the real-time status inside the reactor.

[0074] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in the relevant field should understand that the specific implementation methods of the present invention can still be modified or replaced by equivalents, and any modifications or equivalent replacements that do not depart from the spirit and scope of the present invention should be covered within the scope of protection of the claims of the present invention.

Claims

1. An anaerobic ammonium-oxidizing bacteria cultivation device, comprising: The culture tank structure module, the incubation bed module, the control module and the heat recovery module are characterized by: The culture tank structure module includes: a water inlet mechanism, a culture tank main body mechanism, an internal circulation mechanism and a water outlet mechanism, wherein the water inlet mechanism and the water outlet mechanism are located on both sides of the internal circulation mechanism; a viewing window is also provided on the culture tank main body structure for observing the internal state of the culture tank; The incubation bed module is located inside the main body of the cultivation tank and includes: an incubation tube, graphite and a fiber mesh flower, and is used to inoculate the initial strain of anaerobic ammonia-oxidizing bacteria; The control module is located on one side of the cultivation tank and is used to control the parameters of the inlet and outlet water flow and hydraulic retention time of the device; The heat recovery module is arranged inside and beside the cultivation tank, and includes: an impeller, a piston cylinder, a heat exchange tube and a heat exchanger, which is used to absorb the heat in the water outlet of the device and release the heat in the water inlet mechanism and the incubation tube.

2. The anaerobic ammonium-oxidizing bacteria cultivation device according to claim 1, characterized in that: The water inlet mechanism is composed of a water pump and a water inlet tray. The water inlet tray is located at a height of 20-35% from the bottom to the top of the cultivation tank. The tray surface is composed of a water retainer and a water distribution ring.

3. The anaerobic ammonium-oxidizing bacteria cultivation device according to claim 2, characterized in that: The water retainer can be switched to manual control or an external control mechanism to adjust the shielding position and shielding ratio.

4. The anaerobic ammonium-oxidizing bacteria cultivation device according to claim 1, characterized in that: The culture tank structure consists of an inner tube and an outer tube, the inner diameter difference between the inner tube and the outer tube is 10 to 500 mm, all probes, electric control circuits, and inlet and outlet water pipes of the device are laid to the bottom of the device through the interlayer and finally extend to the outside of the device, and insulation materials are laid between the interlayers.

5. The anaerobic ammonium-oxidizing bacteria cultivation device according to claim 1, characterized in that: The internal circulation mechanism is composed of an internal circulation water inlet pump and a U-shaped annular tube. The annular tube is arranged upright in the main body or arranged at an angle of 0 to 30 degrees to the vertical direction. The inner diameter of the annular tube is 100 to 500 mm, and the total height is 30 to 70% of the height of the tank body. Within the elliptical area, a small hole is opened every 10 to 100 mm, and the aperture of the small hole is 10 to 30 mm. The water inlet and outlet of the U-shaped annular tube are 2 to 8 cm away from the top liquid level.

6. The anaerobic ammonium-oxidizing bacteria cultivation device according to claim 1, characterized in that: The heat recovery module collects the gas generated during the incubation process of the incubation bed module into a collector through the exhaust gas collection port. One end of the collector is immersed in the reactor, and the other end is inside the water outlet pipe. The gas in the collector is compressed by a reciprocating compressor, and then the pressure is released through a pressure relief valve. The released gas is mixed with the newly generated gas and enters the collector again.

7. The anaerobic ammonium-oxidizing bacteria cultivation device according to claim 1, characterized in that: The water outlet mechanism includes a second water inlet, an internal reflux port, an overflow port, a water outlet and a flow path. The water in the reactor first flows into the water outlet through the water inlet, and first reaches the internal reflux port through the internal path. The internal reflux port is connected to the water inlet end of the internal circulation structure, and the water in this area is sucked into the internal circulation structure. The water in the reactor will pass through the overflow port directly to the water outlet.

8. The anaerobic ammonium-oxidizing bacteria cultivation device according to claim 1, characterized in that: The incubation tube comprises several coils, one end of which is placed at the bottom of the device on one side of the water inlet tray. The coils are tied with fluff containing 20-40% graphite ribbon and 60-80% fiber filaments at intervals of 50-300 mm. Cams are arranged at the top and bottom of the coils, and a water outlet mechanism is arranged at the bottom.

9. The anaerobic ammonium-oxidizing bacteria cultivation device according to claim 1, characterized in that: The control module includes any one of an online DO probe, an online ammonia nitrogen probe, an online nitrite probe, an online nitrate probe, an online pH probe, an online pressure gauge, and an online liquid level gauge placed in the reactor.

10. A method for cultivating anaerobic ammonium-oxidizing bacteria, comprising: implementing an anaerobic ammonium-oxidizing bacteria cultivation device according to any one of claims 1 to 9, characterized in that: Step 1, adding activated sludge or anaerobic ammonium oxidation seed sludge with a MLSS concentration of 10 to 1000 mg / L into the reactor, and then letting the culture medium enter the main body of the cultivation tank through the water inlet mechanism of the device under the parameters set by the pre-configured culture medium; Step 2, start the control module, and control the hydraulic retention time of the outlet water according to the changes in the concentrations of ammonia nitrogen, nitrite nitrogen, and nitrate nitrogen in the inlet and outlet water to ensure that the concentration difference of ammonia nitrogen and nitrite nitrogen in the inlet and outlet water is above 50 mg / L; Step 3, regularly start the water pump at the suction end of the water outlet mechanism to ensure smooth overflow of water; Step 4, start the heat recovery device to recover the outlet water heat; Step 5: When the MLSS concentration inside the device is higher than 5000 mg / L, more than half of the internal sludge is discharged, and then the process of steps 2 to 4 is repeated to continue the cultivation of anaerobic ammonia-oxidizing bacteria.

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