Deodorizing and carbon-reducing microbial inoculum as well as preparation method and application thereof

Abstract

The invention relates to a deodorization and carbon reduction bacterial agent as well as a preparation method and application of the deodorization and carbon reduction bacterial agent. The deodorization and carbon reduction bacterial agent comprises pseudomonas stutzeri, paracoccus pantotrophu and massidium sp. X63, and the preparation method comprises the following steps of: adding a microbial inoculum, a microbial inoculum, a microbial inoculum, a microbial inoculum, a microbial inoculum, a microbial inoculum, a microbial inoculum, a microbial inoculum, a microbial inoculum, a microbial inoculum, a microbial inoculum, a microbial inoculum and a microbial inoculum, the Marsinia sp. X63 is preserved in the Korean Center for Type Culture Collection, the preservation number is KCTC: 92384, and the strain can efficiently reduce nitrous oxide. The deodorizing and carbon-reducing microbial inoculum can efficiently remove greenhouse gases including nitrous oxide, methane and carbon dioxide and malodorous gases including dimethyl sulfide and methyl mercaptan, and has a good application prospect in deodorizing and carbon reduction of livestock and poultry farms.

Description

Technical field:

[0001] The present invention relates to the technical field of microorganisms, and in particular to a deodorizing and carbon-reducing bacterial agent and a preparation method and application thereof. Background technology:

[0002] With the rapid development of livestock and poultry farming, the number of large-scale breeding enterprises and scale farms has continued to increase, and it has become one of the important industries for my country's agricultural economic growth. However, farms often use straw, rice husks, etc. as bedding for large-scale laying to achieve the purpose of animal heat preservation and moisture absorption. The large amount of livestock and poultry manure, feed residues, etc. accumulated on these beddings will produce a large amount of odorous gases and greenhouse gases in an anaerobic environment, causing air pollution and causing serious impacts on livestock and poultry and human health. Therefore, controlling the generation of odorous gases and reducing greenhouse gas emissions can not only improve air quality, but also promote the green and sustainable development of livestock and poultry farming.

[0003] At present, the main methods of carbon reduction and deodorization at the end of farms are physical methods, chemical methods and microbial methods. Among them, the physical method mainly uses the adsorption and masking effect of materials to reduce the concentration of exhaust gas or temporarily mask the odor. Commonly used adsorption materials such as biochar, zeolite and silica gel, their adsorption capacity and adsorption performance are easily affected by environmental factors, resulting in unstable physical carbon reduction and deodorization effects; chemical methods mainly use chemical reagents to react with odorous substances to remove odors, but the deodorization effect is not long-lasting and chemical reagents are prone to secondary pollution; microbial methods mainly use the metabolic effect of functional microorganisms to effectively decompose and transform odorous substances to achieve the purpose of deodorization, with the advantages of high efficiency, reusability, no secondary pollution and low cost.

[0004] Since different functional microorganisms can transform different types of malodorous substances, but there are symbiotic, antagonistic and competitive relationships between different microorganisms, the development of efficient deodorizing and carbon-reducing composite microbial agents formed by the synergistic action of different functional microorganisms is still a difficult point in current research. At present, it is necessary to study a composite microbial agent that can efficiently remove malodorous gases and greenhouse gases, thereby improving the deodorizing and carbon-reducing effects of livestock and poultry farms. Summary of the invention:

[0005] The present invention provides a deodorizing and carbon-reducing bacterial agent, a preparation method and application thereof. The deodorizing and carbon-reducing bacterial agent is prepared by taking Pseudomonas aestivus, Paracoccus pantotrophu and Massilia sp. X63 as raw materials, and can effectively reduce malodorous gases and greenhouse gases, thereby achieving the purpose of deodorizing and carbon-reducing.

[0006] The present invention is achieved through the following technical solutions:

[0007] The first object of the present invention is to provide a deodorizing and carbon-reducing bacterial agent, which comprises composite microorganisms and biochar, wherein the composite microorganisms include Pseudomonas stutzeri, Paracoccus pantotrophu and Massilia sp. X63.

[0008] Massilia sp. X63, the strain was deposited in the Korean Collection for Type Cultures (KCTC) on April 28, 2022, address: 181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea, postal code: 56212, and the deposit number is KCTC: 92384.

[0009] The preparation steps of the above-mentioned deodorizing and carbon-reducing bacterial agent are as follows: immersing biochar particles in a microbial nutrient solution and drying to obtain a biochar carrier; then immersing the biochar carrier in a composite microbial bacterial solution, filtering out the bacterial solution after loading the microorganisms, and drying in an oven to obtain the deodorizing and carbon-reducing bacterial agent.

[0010] Preferably, the composite microbial bacterial liquid is obtained by culturing Pseudomonas, Paracoccus pantrophicus and Masseilli X63 in liquid at 30°C and 180 r / min shaking conditions, and then mixing the Pseudomonas bacterial liquid, Paracoccus pantrophicus and Masseilli X63 bacterial liquid.

[0011] Preferably, the Pseudomonas bacterial solution, the Paracoccus pantotrophicus bacterial solution and the Massillaria X63 bacterial solution are mixed in a mass ratio of (1-2):(2-5):(1-3).

[0012] The specific preparation steps of the above-mentioned deodorizing and carbon reducing bacteria agent are as follows:

[0013] (1) placing livestock and poultry waste litter in a tubular furnace for pyrolysis and carbonization into biochar, grinding it into powder in a mortar, and then passing it through a 50-mesh sieve to obtain biochar particles;

[0014] (2) immersing the biochar particles in a microbial nutrient solution, heating to 30-45° C., fully stirring for 2-3 hours, filtering out the nutrient solution, placing in an oven at 30-40° C. and drying until the mass is constant, and then taking out to obtain a biochar carrier;

[0015] (3) The biochar carrier is immersed in the composite microbial bacterial liquid, placed in a shaker at 30-35°C and cultured at 150-200 r / min for 24-48 hours, the biochar is loaded with microorganisms, and the bacterial liquid is filtered out. The biochar is placed in an oven at 30-40°C and dried until the mass is constant, and then taken out to obtain a deodorizing and carbon-reducing bacterial agent.

[0016] Preferably, in step (1), the livestock and poultry breeding waste bedding includes rice husks, straw and livestock and poultry excrement.

[0017] Preferably, in step (1), the conditions for pyrolysis carbonization are: heating from 25°C to 500°C for 1 hour under a nitrogen flow rate of 100 mL / min; then pyrolyzing at 500°C for 1.5 hours; finally cooling from 500°C to 25°C for 0.5 hours to obtain biochar by pyrolysis carbonization.

[0018] Preferably, the composition of the microbial nutrient solution is: 5-8 g / L glucose, 0.8-1.6 g / L ammonium chloride, 5-8 g / L sodium thiosulfate pentahydrate, 0.1-0.5 g / L potassium dihydrogen phosphate, 0.2-1.0 g / L disodium hydrogen phosphate, 0.2-1.0 g / L magnesium sulfate heptahydrate, 0.02-0.1 g / L calcium chloride, and the pH is 6-8.

[0019] Preferably, the weight fraction ratio of the biochar particles to the microbial nutrient solution is 1:(2-3).

[0020] Preferably, the usage ratio of the biochar carrier to the composite microbial culture liquid is 2 g: (2-5) mL.

[0021] The second object of the present invention is to provide the use of the above-mentioned deodorizing and carbon-reducing bacteria agent in removing malodorous gases and greenhouse gases from composting plants and livestock and poultry farms.

[0022] Preferably, the greenhouse gases include carbon dioxide, nitrous oxide and methane, and the malodorous gases include methyl mercaptan and methyl sulfide.

[0023] Preferably, the total viable count of the deodorizing and carbon reducing bacteria agent is ≥10 8 cfu / g.

[0024] The beneficial effects of the present invention are as follows:

[0025] 1. A kind of efficient deodorizing and carbon-reducing bacteria agent provided by the present invention includes three kinds of bacterial strains, namely Pseudomonas stutzeri, Paracoccus pantotrophu and Massiliasp. X63, wherein Massiliasp. X63 is a new strain screened out with efficient removal of nitrous oxide. By loading on biochar after compounding these three strains in a certain proportion, the deodorizing and carbon-reducing bacteria agent obtained can efficiently remove carbon dioxide, nitrous oxide, methane, dimethyl sulfide and methyl mercaptan. When used, it can be spread on the bedding of a composting plant or a livestock and poultry farm, and then the waste gas of the composting plant or the livestock and poultry farm can be preferably removed.

[0026] 2. The present invention provides a highly efficient deodorizing and carbon-reducing bacterial agent that loads synergistic functional microbial flora onto biochar prepared from waste bedding. On the one hand, the nutrients in the nutrient solution impregnated on the biochar help maintain the activity of the microorganisms, improve the efficiency of microbial conversion of harmful substances in the exhaust gas, and maintain the efficient and long-term use of the bacterial agent; on the other hand, it effectively combines the resource utilization of solid waste with gas control and management, which is beneficial to the green circular economy of livestock and poultry farms.

[0027] 3. The present invention provides a highly efficient deodorizing and carbon-reducing bacterial agent, which achieves the purpose of rapid deodorization through biochar adsorption and microbial flora transformation of odors and harmful substances in waste gas. It has the advantages of being environmentally friendly, efficient and free of secondary pollution during actual application.

[0028] Massilia sp. X63, the strain was deposited in the Korean Collection for Type Cultures (KCTC) on April 28, 2022, address: 181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea, postal code: 56212, and the deposit number is KCTC: 92384. Description of the drawings:

[0029] Figure 1 The colony morphology of Masseille X63 on the activated culture medium in Example 2, wherein (A) the colony morphology on the plate; (B) the single colony morphology.

[0030] Figure 2 This is the phylogenetic tree of the genome of Masseilli X63 in Example 3.

[0031] Figure 3 It is the change of nitrous oxide removal efficiency of the deodorizing and carbon-reducing bacteria agent in Example 7 at different times. Specific implementation method:

[0032] It will be appreciated by those skilled in the art that the disclosed techniques in the following examples represent techniques that the inventors have found to work well in the practice of the present invention. However, many changes may be made in the disclosed specific embodiments, and still the same or similar results may be obtained without departing from the spirit and scope of the present invention. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0033] Example 1 Screening and Isolation of Massilia sp. X63

[0034] The Massilia sp. X63 strain of the present invention is obtained by separation and purification from the bottom mud of the mangrove light beach in Hualong Town, Panyu District, Guangzhou City. The separation and purification method is as follows:

[0035] (1) Culture medium:

[0036] Enrichment medium: potassium dihydrogen phosphate 1.5 g / L, disodium hydrogen phosphate 5.0 g / L, magnesium sulfate heptahydrate 0.1 g / L, potassium nitrate 1.0 g / L, sodium succinate 4.7 g / L, pH 7.2.

[0037] Isolation medium: yeast extract 0.5 g / L, tryptone 1.0 g / L, NaCl 0.5 g / L, agar 1.5%.

[0038] Activation culture medium: glucose 5.0 g / L, ammonium chloride 0.8 g / L, sodium thiosulfate pentahydrate 5.0 g / L, potassium dihydrogen phosphate 0.1 g / L, disodium hydrogen phosphate 0.3 g / L, magnesium sulfate heptahydrate 0.2 g / L, pH 7.0.

[0039] (2) Enrichment culture

[0040] Take 1g of mangrove beach mud and put it into a triangular flask containing 100mL of enrichment medium, and culture it at 30℃ and 180r / min for 24h. When the culture solution becomes turbid, take 5mL of culture solution and transfer it to a triangular flask containing 100mL of enrichment medium, and culture it at 30℃ and 180r / min for 24h. Repeat the enrichment 3 times.

[0041] (3) Separation and purification

[0042] The enriched culture solution was diluted to 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 Different concentration gradients were used, and 0.2 mL of bacterial solution of different concentration gradients was applied to the separation medium, and cultured in a 30°C constant temperature incubator until obvious single colonies appeared. Single colonies with different morphologies were picked and transferred to the separation medium on the slant of the test tube, cultured at 30°C for 48 hours, and then transferred to a 4°C refrigerator for storage. A total of 11 purified strains were obtained according to the above separation method.

[0043] (4) Functional evaluation

[0044] The above purified strains were inoculated into the activation medium and cultured at 30℃, 180r / min shaking table for 24h. When the culture solution became turbid, 8mL of bacterial solution was taken into a 10mL centrifuge tube, centrifuged at 8000r / min for 5min, washed twice with PBS buffer, and then fully suspended by adding 5mL PBS buffer. The suspension was aspirated into a headspace bottle containing 30mL of enriched culture medium without potassium nitrate and sealed; at the same time, 35mL of enriched culture medium without potassium nitrate was added to the headspace bottle as a blank control. Finally, 893.6μg / L of nitrous oxide gas was filled, and the mixture was placed in a shaker at 30℃, 180r / min for 24h and then allowed to stand. The concentration of nitrous oxide gas in the headspace bottle was determined by gas chromatograph, and the removal rate of nitrous oxide in the treatment group was calculated compared with the blank control. After screening, nitrous oxide-reducing bacteria X63 were finally obtained, with a removal rate of nitrous oxide of 74.2%.

[0045] Example 2 Identification of nitrous oxide reducing bacteria X63

[0046] Gram staining experiments proved that strain X63 was a Gram-negative bacterium. Strain X63 was inoculated on the activation medium and cultured at 30℃ for 48 hours before morphological observation. The surface of its single colony was smooth, yellow, and rod-shaped ( Figure 1 ).

[0047] DNA was extracted from strain X63, and its 16S rRNA gene fragment was amplified and sequenced using bacterial universal primers 1492R and 27F. The two forward and reverse sequences were spliced ​​using Contig Express software to obtain a complete sequence, which is shown in SEQ ID No: 1 and is 1352 bp long. BLAST was used to compare the sequence with the sequences registered in the GenBank database for homology analysis. The results showed that strain X63 was similar to the model strain Masslia yuzhufengenisiY1243-1 T The highest similarity was 98.67%; the 16S rRNA gene sequence similarity with other type strains of the genus Massilia was lower. These results showed that strain X63 was similar to the type strain Masslia yuzhufengenisiY1243-1 T According to the results of 16SrRNA gene sequence comparison, strain X63 belongs to the genus Masseella, and its taxonomic status needs to be determined in combination with its genome analysis.

[0048] Example 3 Genome Analysis of Massilia sp. X63

[0049] The genome of strain X63 extracted in Example 2 was sent to Sangon Biotech (Shanghai) Co., Ltd., and the genome was sequenced using the Illumina NovaSeq platform, and then the genome sequence was spliced ​​using SPAdes v3.15.3 software, and finally the genome integrity and contamination degree were evaluated using CheckM software. The results showed that the total genome length of strain X63 was 5.72 Mbp, the G+C content of genomic DNA was 66.2 mol%, the genome integrity was 99.6%, and the contamination degree was 1.4%.

[0050] The phylogenetic tree of the genome of strain X63 was constructed using UBCG software. The results showed that ( Figure 2 ), strain X63 and model strain MassiliauzhufengenisiY1243-1 T and Massilia agri K-3-1 T The online tool ANI was used to calculate the average nucleotide identity (ANI) between the genome sequences of strain X63 and the model strains of closely related species. The results showed that strain X63 and the closely related model strain MassiliayuzhufengenisiY1243-1 T and Massilia agri K-3-1 T The ANI values ​​of strain X63 and strain X63 were 85.25% and 85.14%, respectively, which were lower than the ANI threshold (95% to 96%) for defining new species of prokaryotes. Therefore, the above results indicate that strain X63 is a potential new species of Massilia sp.

[0051] The strain X63 was named Massilia sp. X63 and was deposited in the Korean Collection for Type Cultures (KCTC) on April 28, 2022. The address is: 181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea, postal code: 56212, and the deposit number is KCTC: 92384.

[0052] Example 4 Preparation of biochar carrier

[0053] This embodiment provides a biochar carrier, which is prepared by the following steps:

[0054] The waste litter from the chicken farm was dried in an oven at 80°C to constant weight and then placed in a tube furnace. The waste litter was pyrolyzed and carbonized into biochar under a nitrogen flow rate of 100 mL / min. The tube furnace conditions were: heating stage 25 to 500°C, time 1h; pyrolysis temperature 500°C, time 1.5h; cooling stage 500 to 25°C, time 0.5h. After pyrolysis and carbonization, it was ground into powder in a mortar and passed through a 50-mesh sieve to obtain biochar particles.

[0055] The biochar particles were immersed in a microbial nutrient solution at a weight fraction ratio of 1:2, the composition of the nutrient solution was: 5g / L glucose, 0.8g / L ammonium chloride, 5g / L sodium thiosulfate pentahydrate, 0.1g / L potassium dihydrogen phosphate, 0.3g / L disodium hydrogen phosphate, 0.3g / L magnesium sulfate heptahydrate, 0.03g / L calcium chloride, pH 6 to 8. Then heated to 30 to 45°C, stirred for 2 to 3 hours, filtered off the nutrient solution, placed in a 30 to 40°C oven to dry until the mass was constant, and then taken out to obtain a biochar carrier.

[0056] Comparative Example 1

[0057] This comparative example provides a biochar carrier, referring to Example 4, except that the tubular furnace pyrolysis temperature of 500° C. is changed to 700° C., and the remaining raw materials and preparation process are the same as Example 4.

[0058] Comparative Example 2

[0059] This comparative example provides a biochar carrier, referring to Example 4, except that the tubular furnace pyrolysis temperature of 500° C. is changed to 900° C., and the remaining raw materials and preparation process are the same as Example 4.

[0060] Example 5 Preparation of a deodorizing and carbon-reducing bacterial agent

[0061] 5% of the above-screened Massilia sp. X63 was inoculated into the activation medium (same as in Example 1) cooled to room temperature, and cultured at 30°C and 180 r / min shaking conditions for 24 hours to obtain a Massilia sp. bacterial liquid. The biochar carrier of Example 4 and the obtained Massilia sp. bacterial liquid were mixed evenly at a ratio of 2g:5mL, placed in a shaking table at 30°C and cultured at 180 r / min for 24 hours, and the bacterial liquid was filtered out after the biochar was loaded with microorganisms, and placed in a 35°C oven to dry until the mass was constant, and then taken out to obtain a deodorizing and carbon-reducing bacterial agent.

[0062] Example 6 Preparation of a deodorizing and carbon-reducing bacterial agent

[0063] Preparation of composite microbial liquid: Pseudomonas liquid, Paracoccus pantrophus liquid and Massimo X63 liquid were mixed at a mass ratio of 1:1:1 to form a composite microbial liquid. The biochar carrier of Example 4 and the obtained composite microbial liquid were mixed at a ratio of 2g:5mL, placed in a shaker at 30°C and cultured at 180r / min for 24 hours, the biochar was loaded with microorganisms, and the composite microbial liquid was filtered out, placed in a 35°C oven to dry until the mass was constant, and then taken out to obtain a deodorizing and carbon-reducing bacterial agent.

[0064] The Pseudomonas bacterial liquid is prepared by the following steps: 5% Pseudomonas (Pseudomonas stutzeri) purchased from Guangdong Microbiological Culture Collection Center, with a collection number of GDMCC 1.2525, is inoculated into an activation culture medium cooled to room temperature, and cultured at 30°C and 180 r / min shaking for 24 hours to obtain the Pseudomonas bacterial liquid.

[0065] The paracoccus pantotrophus bacterial liquid is prepared by the following steps: 5% paracoccus pantotrophu purchased from Guangdong Microbiological Culture Collection Center with the accession number GDMCC1.433 is inoculated into the activation culture medium cooled to room temperature, and cultured at 30°C and 180 r / min shaking conditions for 24 hours to obtain the paracoccus pantotrophus bacterial liquid.

[0066] The Massilia sp. X63 bacterial liquid is prepared by the following steps: 5% of the above-screened Massilia sp. X63 is inoculated into an activation culture medium cooled to room temperature, and cultured at 30° C. and 180 r / min shaking for 24 hours to obtain the Massilia sp. X63 bacterial liquid.

[0067] Example 7 Preparation of a deodorizing and carbon-reducing bacterial agent

[0068] This embodiment provides a deodorizing and carbon reducing bacterial agent, referring to Example 6, except that the ratio of each bacterial solution in the composite microbial bacterial solution is different, that is, the Pseudomonas bacterial solution, the Paracoccus pantotrophicus bacterial solution and the Massillaria X63 bacterial solution are mixed uniformly in a mass ratio of 2:5:3 to form a composite microbial bacterial solution. The other steps are the same as in Example 6.

[0069] The biochar carriers obtained in Example 4, Comparative Example 1 and Comparative Example 2 and the deodorizing and carbon-reducing bacteria agents prepared in Example 5 to Example 7 were tested for their greenhouse gas and malodor gas removal effects. The test process is as follows:

[0070] Weigh 0.1g of biochar carrier or deodorizing carbon-reducing bacteria agent in a 100mL serum bottle, and inject a quantitative amount of greenhouse gas (methane, nitrous oxide, carbon dioxide mixed gas) after the gland is sealed. After 18h after the gland is sealed, take samples and use gas chromatography to test the concentrations of methane, nitrous oxide and carbon dioxide respectively; In addition, weigh 0.1g of deodorizing carbon-reducing bacteria agent in a 100mL serum bottle, and inject a quantitative amount of malodorous gas (methyl sulfide and methyl mercaptan mixed gas) after the gland is sealed. After 18h after the gland is sealed, take samples and use gas chromatography to test the concentrations of methyl sulfide and methyl mercaptan. Determine the changes in concentrations before and after different gas treatments, and calculate their removal rates by the following formula: Gas removal rate = (initial concentration-concentration after treatment) / initial concentration × 100%. The test results are shown in Table 1:

[0071] Table 1

[0072]

[0073] As can be seen from Table 1, the biochar carrier prepared in Example 4 has the highest adsorption efficiency for malodorous gases (methyl sulfide and methyl mercaptan) compared with Comparative Examples 1 and 2, and the lowest adsorption efficiency for methane in greenhouse gases, but there is no significant difference in the adsorption efficiency for nitrous oxide and carbon dioxide. Considering energy consumption and economy, the biochar carrier prepared at a pyrolysis temperature of 500°C in Example 4 has the best comprehensive effect. Comparing Examples 5, 6 and 7, the deodorizing and carbon-reducing bacterial agent prepared by the composite microbial bacterial liquid has a significant gain effect on the removal of nitrous oxide, methane and methyl mercaptan gases compared with the single Marseille bacterial liquid. In Example 7, the Pseudomonas bacterial liquid, the pantrophic Paracoccus bacterial liquid and the Marseille bacterial liquid are mixed in a mass ratio of 2:5:3 to form a composite microbial bacterial liquid loaded on the biochar carrier prepared in Example 4, and the deodorizing and carbon-reducing effect of the bacterial agent is the best.

[0074] SEQ ID NO.1 (16S rRNA sequence of Marseilles X63):

[0075]

Claims

1. A deodorizing and carbon reducing bacterial agent, characterized in that: The invention comprises composite microorganisms and biochar, wherein the composite microorganisms include Pseudomonas stutzeri, Paracoccus pantotrophu and Massilia sp. X63, and the preservation number of Massilia sp. X63 is KCTC:92384.

2. The bacterial agent according to claim 1, characterized in that The preparation steps are as follows: immersing biochar particles in a microbial nutrient solution and drying to obtain a biochar carrier; then immersing the biochar carrier in a composite microbial bacterial solution, filtering out the bacterial solution after loading the microorganisms, and drying in an oven to obtain a deodorizing and carbon-reducing bacterial agent.

3. The bacterial agent according to claim 2, characterized in that The composite microbial bacterial liquid is obtained by culturing Pseudomonas, Paracoccus pantrophicus and Masseilli X63 in liquid at 30°C and 180 r / min shaking conditions, and then mixing the Pseudomonas bacterial liquid, Paracoccus pantrophicus and Masseilli X63 bacterial liquid.

4. The bacterial agent according to claim 3, characterized in that The Pseudomonas bacterial solution, the Paracoccus pantrophus bacterial solution and the Massillaria X63 bacterial solution were mixed in a mass ratio of (1-2):(2-5):(1-3).

5. The bacterial agent according to claim 2, characterized in that The specific preparation steps are: (1) placing livestock and poultry waste litter in a tubular furnace for pyrolysis and carbonization into biochar, grinding it into powder in a mortar, and then passing it through a 50-mesh sieve to obtain biochar particles; (2) immersing the biochar particles in a microbial nutrient solution, heating to 30-45° C., fully stirring for 2-3 hours, filtering out the nutrient solution, placing in an oven at 30-40° C. and drying until the mass is constant, and then taking out to obtain a biochar carrier; (3) The biochar carrier is immersed in the composite microbial bacterial liquid, placed in a shaker at 30-35°C and cultured at 150-200 r / min for 24-48 hours, the biochar is loaded with microorganisms, and the bacterial liquid is filtered out. The biochar is placed in an oven at 30-40°C and dried until the mass is constant, and then taken out to obtain a deodorizing and carbon-reducing bacterial agent.

6. The bacterial agent according to claim 5, characterized in that In step (1), the livestock and poultry breeding waste bedding includes rice husks, straw and livestock and poultry excrement; the pyrolysis carbonization conditions are: heating from 25°C to 500°C under a nitrogen flow rate of 100 mL / min for 1 hour; then pyrolyzing at 500°C for 1.5 hours; finally cooling from 500°C to 25°C for 0.5 hours, and pyrolyzing to obtain biochar.

7. The bacterial agent according to claim 2 or 5, characterized in that: The microbial nutrient solution is composed of: 5-8 g / L glucose, 0.8-1.6 g / L ammonium chloride, 5-8 g / L sodium thiosulfate pentahydrate, 0.1-0.5 g / L potassium dihydrogen phosphate, 0.2-1.0 g / L disodium hydrogen phosphate, 0.2-1.0 g / L magnesium sulfate heptahydrate, 0.02-0.1 g / L calcium chloride, and a pH of 6-8.

8. The bacterial agent according to claim 2 or 5, characterized in that: The weight fraction ratio of the biochar particles to the microbial nutrient solution is 1:(2-3).

9. The bacterial agent according to claim 2 or 5, characterized in that: The usage ratio of the biochar carrier to the composite microbial liquid is 2 g: (2-5) mL.

10. Use of the deodorizing and carbon-reducing bacterial agent according to any one of claims 1 to 9 in removing malodorous gases and greenhouse gases from composting plants and livestock and poultry farms.

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