Grape seed development gene VvPHERES1 and application thereof

By cloning and overexpressing the grape seed development gene VvPHERES1, the problem of low efficiency and low probability of breeding of seedless grapes in the prior art was solved, and a significant increase in seed number was achieved, providing technical support for molecular breeding of seedless grapes.

CN119685345AActive Publication Date: 2025-03-25XIAN BOTANICAL GARDEN SHAANXI PROV
4 Cites 3 Cited by

Patent Information

Application Number
CN202411984889.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-31
Publication Date
2025-03-25
Estimated Expiration
2044-12-31

AI Technical Summary

Technical Problem

The prior art has a long breeding cycle, low breeding efficiency and low probability of nucleus-free offspring when breeding seedless grapes, and lacks genes that can regulate the anuclearity of grape seeds.

Method used

The grape seed development-related gene VvPHERES1 was cloned for the first time, and its localization was performed in the nucleus by subcellular analysis, and its expression differences in nucleus and nucleus-free grape ovule were analyzed in situ hybridization to construct VvPHERES1 overexpression vector, overexpressed in tomatoes, and the increase in seed number was observed.

Benefits of technology

Through the overexpression of the VvPHERES1 gene, the number of seeds of tomatoes has been significantly increased, providing a basis for molecular breeding of seedless grapes.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention discloses a grape seed development gene VvPHERES1 and an application of the grape seed development gene VvPHERES1. The nucleotide sequence of the developmental gene VvPHERES1 is as shown in SEQ ID NO: 1. The amino acid sequence of the TypeI MADS transcription factor is as shown in SEQ ID NO: 2. According to the invention, the grape seed development related gene VvPHERES1 is cloned for the first time, and the gene function of the grape seed development related gene VvPHERES1 is researched. The method comprises the following steps: constructing a pCAMBIA2300-VvPHERES1 overexpression vector, and carrying out heterologous transformation on a Micro-Tom tomato plant. By comparing the change of seed characters in the overexpressed transgenic tomato and the wild type plant, the result shows that the number of seeds in the overexpressed transgenic tomato is obviously increased. The invention provides a gene related to regulation and control of grape seed development, and a basis is provided for seed selection of new seedless grape varieties.
Need to check novelty before this filing date? Find Prior Art