Device for determining denitrification rate of bottomland by in-situ layering multi-method

Abstract

The device comprises a vertical square column body with an opening in the bottom and a hollow interior, triangular plates with downward tips are fixedly connected to the lower ends of the front side face and the rear side face of the vertical square column body, a plurality of layering seams are formed in the left side face of the vertical square column body, and layering insertion plate rails corresponding to the layering seams are arranged on the inner side of the vertical square column body; layered insertion plates which are matched with the layered insertion plate tracks and divide the internal space of the column body into a plurality of closed culture bins are arranged, and each culture bin is provided with two gas sampling bins and a culture bin injection device. The device can realize in-situ layered nitrogen removal rate experiment, can simultaneously meet the requirements of two nitrogen removal experiment modes of an acetylene inhibition method and an isotopic tracer method, can meet the requirements of nitrogen removal microorganism structure and abundance measurement, can realize relatively good matching degree of the in-situ nitrogen removal rate and in-situ physical and chemical data, and can be applied to the field of nitrogen removal. The method has high practical value and popularization prospect for understanding and mastering the nitrogen cycle process in the beach land and evaluating the ecological environment benefit of the beach land.

Description

Technical Field

[0001] The invention belongs to the field of beach environment monitoring, and in particular relates to a device for measuring denitrification rate of a beach by in-situ stratification and multiple methods. Background Art

[0002] Beaches are key connecting zones between land and water (river and lake shores, coastal beaches), and are sensitive areas where hydrological, chemical, and ecological processes interact in aquatic ecosystems. They have a high vertical abundance of denitrifying and anaerobic ammonium oxidation (ANAMMOX) denitrifying microorganisms. It has been reported that denitrification and ANAMMOX (contributing up to 37.5-67.6%) dominate the denitrification of saturated aquifers and unsaturated soil layers, respectively (Zhu et al, Scientific Reports, 209), playing an important role in maintaining nitrogen balance in regional and even global aquatic ecosystems.

[0003] However, current research on the denitrification process of tidal flats often uses in-situ layered mud samples (including pore water samples) to bring back to the laboratory for indoor denitrification rate measurement experiments, and adopts acetylene inhibition method or isotope tracer method according to the experimental objectives. However, there are two main problems with indoor experiments. On the one hand, the physical and chemical indicators and redox conditions of the mud samples (including pore water samples) collected in situ have undergone significant changes. On the other hand, the abundance and structure of denitrification functional microorganisms in the samples may also change significantly due to changes in environmental conditions. The experimental results are difficult to truly reflect the in-situ denitrification conditions and actual denitrification rate, and it is difficult to provide guidance and reference for scientifically measuring the denitrification contribution within the tidal flat and evaluating the corresponding ecological value. Summary of the Invention

[0004] In response to the above-mentioned problems of the prior art, the present invention designs a device for in-situ stratification and multi-method determination of denitrification rate in beaches, so as to solve the current problem of difficulty in conducting in-situ stratification and multi-method determination of denitrification rate experiments in beaches.

[0005] This device enables in-situ stratified denitrification rate experiments and can simultaneously meet the needs of both acetylene inhibition and isotope tracing methods. Furthermore, it features simultaneous analysis of key indicators influencing denitrification rates (total nitrogen, nitrate nitrogen, and temperature) and mud sampling (for subsequent determination of the structure and abundance of denitrifying microorganisms). This allows for a close match between in-situ denitrification rates and in-situ physical and chemical data, providing guidance and data reference for scientifically measuring denitrification contributions and impact mechanisms within floodplains, as well as for assessing their ecological value. This meets the needs for in-depth analysis of the mechanisms influencing in-situ stratified denitrification rates.

[0006] In order to achieve the above object, the technical solution adopted by the present invention is as follows: A device for determining denitrification rate of beach in situ stratification by multiple methods, comprising a column 2, a gas sampling chamber and a culture chamber injection device 9, The column 2 is a vertical square column with an open bottom and a hollow interior. The lower ends of the front and rear sides of the column 2 are fixedly connected to a triangular plate 5 with the tip pointing downward. A plurality of layered seams 4 are provided on the left side of the column 2. Layered plug-in rails 3 are provided on the inner sides of the front and rear sides respectively corresponding to the layered seams 4. A layered plug-in plate 17 is provided to match each pair of the layered plug-in rails 3. The layered plug-in plates 17 pass through the layered seams 4. The layered inserts 17 divide the interior space of the column 2 into several closed culture chambers 6. Two gas sampling chambers and a culture chamber injection device 9 are respectively provided on the front side corresponding to each culture chamber 6. The right side surface of the column 2 is hinged to the front side surface or the rear side surface.

[0007] The triangular plate 5 is used for piercing into the soft beach.

[0008] The top of the column 2 is fixedly connected with a depth-fixing push rod 1, which can assist in pushing the column 2 into the mud on the beach.

[0009] The right side can be opened to open the column 2 after the experiment is completed.

[0010] The right side is locked by a cylinder lock 10.

[0011] The gas sampling chamber includes a gas chamber 73, a breathable membrane 74, a sliding chamber door 75, and a sealing ring 76. The chamber opening of the gas chamber 73 is closed by the breathable membrane 74. A sealing ring 76 is provided between the chamber opening of the gas chamber 73 and the breathable membrane 74. The cross-section of the sealing ring 76 is a U-shape with an opening facing the centripetal. The top of the sealing ring 76 has an opening that cooperates with the sliding chamber door 75. When the sliding chamber door 75 is open, the gas chamber 73 is connected to the culture chamber 6 through the inner ring of the sealing ring 76. The gas chamber 73 is fixedly connected to the front side of the column 2.

[0012] The gas sampling chamber further includes a first pusher 71 and a chamber door push rod 72. The first pusher 71 includes a chamber door transmission motor 711, a chamber door transmission gear 712, and a chamber door screw 713. The door screw 713 and the door push rod 72 are connected by threaded rotation, the door transmission motor 711 is connected to the door screw 713 through the door transmission gear 712, and the door push rod 72 is fixedly connected to the top of the sliding door 75 to realize the opening or closing of the sliding door 75.

[0013] The initial state of the gas chamber 73 is vacuum. The sliding chamber door 75 can be opened and closed up and down under the pushing and pulling action of the first pusher 71 and the chamber door push rod 72. The breathable membrane 74 can prevent the mud sample from entering and allow the gas in the culture chamber 6 to enter the gas chamber 73. The sealing ring 76 can ensure that the gas chamber 73 is airtight when the sliding chamber door 75 is closed. The gas chamber 73 is fixed to the front side of the column 2 and is used to collect nitrous oxide and nitrogen produced in the experiment in the culture chamber 6.

[0014] The culture chamber injection device 9 includes a gas-liquid chamber 94 and a piston 93 for compressing the gas-liquid chamber 94 . The gas-liquid chamber 94 is connected to the interior of the culture chamber 6 through an injection needle 95 .

[0015] The culture chamber injection device 9 also includes a second pusher 91 and a piston push rod 92. The second pusher 91 includes a piston transmission motor 911, a piston transmission gear 912, and a piston screw 913. The piston transmission motor 911 is connected to the piston screw 913 through the piston transmission gear 912. The piston screw 913 and the piston push rod 92 are connected by threaded rotation. The piston push rod 92 is fixedly connected to the piston 93 to realize the compression of the gas-liquid chamber 94 by the piston 93.

[0016] The second pusher 91 pushes the piston push rod 92 and the piston 93 to inject the acetylene gas or isotope tracer in the gas-liquid tank 94 into the culture tank 6 .

[0017] The device for in-situ stratified multi-method determination of denitrification rate in beach is also provided with a pore water collection and analysis system, including a pore water filter membrane 11, a pore water sampling hole 12, a pore water sampling shell 13, a pore water sampling tube 14, and a pore water collection and analysis device 15. The pore water collection and analysis device 15 includes a pore water sampling bottle 157. The pore water sampling hole 12 is arranged in the middle of the pore water sampling shell 13. The pore water sampling hole 12 is connected to one end of the pore water sampling tube 14 through the pore water filter membrane 11. The other end of the pore water sampling tube 14 is connected to the pore water sampling bottle 157. The pore water sampling shell 13 is fixed to the right side of the column 2.

[0018] The pore water collection and analysis device 15 also includes a centralized power supply 151, a pump 152, a water outlet pipe 153, a total nitrogen probe 154, a nitrate nitrogen probe 155, and a water temperature probe 156. The centralized power supply 151 is used for centralized power supply of the device. The pore water sampling tube (14) is connected to the pore water sampling bottle 157 through the pump 152 and the water outlet pipe 153 in sequence. When extracting, the stratified pore water enters the pore water sampling bottle 157 through the water outlet pipe 153. The total nitrogen probe 154, the nitrate nitrogen probe 155, and the water temperature probe 156 are set on the side of the pore water sampling bottle 157 for real-time analysis of key indicators affecting the stratified denitrification rate.

[0019] The apparatus for in-situ stratified multi-method determination of denitrification rate in beach land further includes a control panel 16 , which includes a signal receiver 161 and a display screen 162 . The signal receiver 161 is communicatively connected to the total nitrogen probe 154 , the nitrate nitrogen probe 155 , and the water temperature probe 156 , for displaying the analysis results of the indicators.

[0020] The control panel 16 also includes a culture chamber control button 163 and a direction button 164. The culture chamber control button 163 and / or the direction button 164 are communicatively connected to the piston drive motor 911 and / or the chamber door drive motor 711 for controlling the gas sampling chamber and the culture chamber injection device 9 of the device.

[0021] During the experiment, each culture chamber 6 corresponds to two gas sampling chambers, one for the initial gas sampling chamber 7 and the other for the final gas sampling chamber 8. The operator can pre-select to load acetylene gas or isotope tracer into the gas-liquid chamber 94 of the culture chamber injection device 9 according to the requirements of the in-situ denitrification experimental method, and make the gas chamber 73 vacuum and close the sliding chamber door 75. After completing the above-mentioned experimental equipment layout, the operator pushes the fixed depth push rod 1 to vertically penetrate the experimental device into the beach, so that the interior of the column 2 is filled with beach mud samples. Then the operator slowly pulls out the column 2 vertically, and inserts the layered plug 17 into the column 2 along the layered seam 4 and the layered plug track 3 on the left side of the column 2, so that the upper and lower layers of the layered plug 17 and the side wall of the column 2 form a cubic culture chamber. The column 2 that has completed the layering is slowly penetrated into the in-situ hole of the beach again through the fixed depth push rod 1 to meet the temperature, humidity, low oxygen and other conditions required for the in-situ experiment. The control panel 16 remotely controls the culture chamber injection device 9 on the culture chamber 6 to inject acetylene gas or isotope tracer from the gas-liquid reservoir 94 into the stratified culture chamber 6. The initial gas sampling chamber 7 (initial vacuum state) on the culture chamber 6 is activated by the door drive motor 711, which opens its sliding door 75. After the initial gases (nitrous oxide and nitrogen) enter the culture chamber 6, the sliding door 75 is closed. After the in-situ denitrification experiment in the stratified culture chamber 6 has completed a predetermined duration (typically 24 hours), the terminating gas sampling chamber 8 (initial vacuum state, identical in structure to the initial gas sampling chamber 7) on the culture chamber 6 is remotely controlled and, under the control of the door drive motor, opens its sliding door. After the terminating gases enter the culture chamber 6, the sliding door is closed, completing the sampling of the terminating gases (nitrous oxide and nitrogen). Upon completion of the in-situ denitrification experiment, pump 151 is activated to extract the stratified pore water. The extracted pore water is then placed into pore water sampling bottles 157 via pore water sampling tubes 14. Total nitrogen, nitrate nitrogen, and water temperature are analyzed using total nitrogen probes 154, nitrate nitrogen probes 155, and water temperature probes 156. After the in-situ denitrification experiment and pore water testing are complete, the operator pulls up the depth-setting push rod 1, slowly raising the column 2 to the surface. The operator then unlocks the column 2 lock 10, removes mud samples from different depths, places them into sample bottles, and cools them with liquid nitrogen for storage. Furthermore, a 15ml syringe is used to extract gas samples from the initial and final gas sampling chambers 7 and 8, respectively, and places them into vacuum bottles for testing. Finally, the collected in-situ gas, mud, and pore water samples are brought back to the laboratory for further testing of gas concentration (for calculating denitrification rates), physical and chemical parameters, and microbial abundance.

[0022] In summary, the structure of this device has the advantage of modular combination and wider application scenarios. It solves the current problem of difficulty in conducting in situ denitrification rate experiments in vertical stratification on beaches, and meets the needs of in situ stratification determination of denitrification rates by various methods such as acetylene inhibition method and isotope tracer method. At the same time, it also has the functions of synchronous analysis of the main indicators affecting the denitrification rate (total nitrogen, nitrate nitrogen, temperature) and mud sample collection (for subsequent determination of denitrification microbial structure and abundance), achieving a good match between in situ denitrification rate and in situ physical and chemical data, meeting the needs of in-depth analysis of the influencing mechanism of in situ stratified denitrification rate, and providing guidance and data reference for scientifically measuring the denitrification contribution within the beach and evaluating the ecological value of the beach, and has high promotion and application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The drawings in the specification, which constitute a part of this application, are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0024] Figure 1 It is a schematic structural diagram of a device for in-situ stratified multi-method determination of denitrification rate in a beach in Example 1 of the present invention.

[0025] Figure 2 It is a schematic diagram of the three-dimensional structure of the column in Example 1.

[0026] Figure 3 This is a schematic diagram of the structure of the initial gas sampling chamber in Example 1 (the termination gas sampling chamber has the same structure), wherein the left figure is a schematic diagram of the structure of the sliding chamber door in the closed state, and the right figure is a schematic diagram of the structure of the sliding chamber door in the open state.

[0027] Figure 4 It is a structural diagram of the first pusher in Example 1.

[0028] Figure 5 It is a structural schematic diagram of the culture chamber injection device in Example 1.

[0029] Figure 6 It is a structural diagram of the second pusher in Example 1.

[0030] Among them, 1 is a fixed depth push rod, 2 is a column, 3 is a layered plug track, 4 is a layered seam, 5 is a triangular plate, 6 is a culture chamber, 7 is an initial gas sampling chamber, 71 is a first pusher, 72 is a chamber door push rod, 73 is a gas chamber, 74 is a breathable membrane, 75 is a sliding chamber door, 76 is a sealing ring, 711 is a chamber door drive motor, 712 is a chamber door drive gear, 713 is a chamber door screw, 8 is a termination gas sampling chamber, 9 is a culture chamber injection device, 91 is a second pusher, 92 is a piston push rod, 93 is a piston, 94 is a gas-liquid chamber, 95 is an injection needle, 911 is a piston drive Motor, 912 is the piston transmission gear, 913 is the piston screw, 10 is the column lock, 11 is the pore water filter membrane, 12 is the pore water sampling hole, 13 is the pore water sampling shell, 14 is the pore water sampling tube, 15 is the pore water collection and analysis device, 151 is the centralized power supply, 152 is the pump, 153 is the water outlet pipe, 154 is the total nitrogen probe, 155 is the nitrate nitrogen probe, 156 is the water temperature probe, 157 is the pore water sampling bottle, 16 is the control panel, 161 is the signal receiver, 162 is the display screen, 163 is the culture chamber control button, 164 is the direction button, and 17 is the layered plug board. DETAILED DESCRIPTION

[0031] It should be noted that, in the absence of conflict, the embodiments and features of the embodiments in this application can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and in combination with the embodiments. Example 1

[0032] like Figures 1 to 6 The device shown is used for in-situ stratified multi-method determination of denitrification rate in a beach, comprising a column 2, an initial gas sampling chamber 7, a final gas sampling chamber 8 and a culture chamber injection device 9. The column 2 is a vertical square column with an open bottom and a hollow interior. The lower ends of the front and rear sides of the column 2 are fixedly connected to a triangular plate 5 with the tip pointing downward. A plurality of layered seams 4 are provided on the left side of the column 2. Layered plug-in rails 3 are provided on the inner sides of the front and rear sides of the column 2 corresponding to the layered seams 4. A layered plug-in plate 17 is provided to match each pair of the layered plug-in rails 3. The layered plug-in plates 17 pass through the layered seams 4. The layered inserts 17 divide the interior space of the column 2 into several closed culture chambers 6. Two gas sampling chambers and a culture chamber injection device 9 are respectively provided on the front side corresponding to each culture chamber 6. The right side surface of the column 2 is hinged to the front side surface or the rear side surface.

[0033] The triangular plate 5 is used for piercing into the soft beach.

[0034] The top of the column 2 is fixedly connected with a depth-fixing push rod 1, which can assist in pushing the column 2 into the mud on the beach.

[0035] The right side can be opened to open the column 2 after the experiment is completed.

[0036] The right side is locked by a cylinder lock 10.

[0037] The gas sampling chamber includes a gas chamber 73, a breathable membrane 74, a sliding chamber door 75, and a sealing ring 76. The chamber opening of the gas chamber 73 is closed by the breathable membrane 74. A sealing ring 76 is provided between the chamber opening of the gas chamber 73 and the breathable membrane 74. The cross-section of the sealing ring 76 is a U-shape with an opening facing the centripetal. The top of the sealing ring 76 has an opening that cooperates with the sliding chamber door 75. When the sliding chamber door 75 is open, the gas chamber 73 is connected to the culture chamber 6 through the inner ring of the sealing ring 76, and the gas chamber 73 is fixedly connected to the front side.

[0038] The gas sampling chamber also includes a first pusher 71 and a chamber door push rod 72. The first pusher 71 includes a chamber door transmission motor 711, a chamber door transmission gear 712, and a chamber door screw 713. The chamber door screw 713 and the chamber door push rod 72 are connected by threaded rotation. The chamber door transmission motor 711 is connected to the chamber door screw 713 through the chamber door transmission gear 712. The chamber door push rod 72 is fixedly connected to the top of the sliding chamber door 75 to realize the opening or closing of the sliding chamber door 75.

[0039] The initial state of the gas chamber 73 is vacuum. The sliding chamber door 75 can be opened and closed up and down under the pushing and pulling action of the first pusher 71 and the chamber door push rod 72. The breathable membrane 74 can prevent the mud sample from entering and allow the gas in the culture chamber 6 to enter the gas chamber 73. The sealing ring 76 can ensure that the gas chamber 73 is airtight when the sliding chamber door 75 is closed. The gas chamber 73 is fixed to the front side of the column 2 and is used to collect nitrous oxide and nitrogen produced in the experiment in the culture chamber 6.

[0040] The culture chamber injection device 9 includes a gas-liquid chamber 94 and a piston 93 for compressing the gas-liquid chamber 94 . The gas-liquid chamber 94 is connected to the interior of the culture chamber 6 through an injection needle 95 .

[0041] The culture chamber injection device 9 also includes a second pusher 91 and a piston push rod 92. The second pusher 91 includes a piston transmission motor 911, a piston transmission gear 912, and a piston screw 913. The piston transmission motor 911 is connected to the piston screw 913 through the piston transmission gear 912. The piston screw 913 and the piston push rod 92 are connected by threaded rotation. The piston push rod 92 is fixedly connected to the piston 93 to realize the compression of the gas-liquid chamber 94 by the piston 93.

[0042] The second pusher 91 pushes the piston push rod 92 and the piston 93 to inject the acetylene gas or isotope tracer in the gas-liquid tank 94 into the culture tank 6 .

[0043] The device for in-situ stratified multi-method determination of denitrification rate in beach is also provided with a pore water collection and analysis system, including a pore water filter membrane 11, a pore water sampling hole 12, a pore water sampling shell 13, a pore water sampling tube 14, and a pore water collection and analysis device 15. The pore water collection and analysis device 15 includes a pore water sampling bottle 157. The pore water sampling hole 12 is arranged in the middle of the pore water sampling shell 13. The pore water sampling hole 12 is connected to one end of the pore water sampling tube 14 through the pore water filter membrane 11. The other end of the pore water sampling tube 14 is connected to the pore water sampling bottle 157. The pore water sampling shell 13 is fixed to the right side of the column 2.

[0044] The pore water collection and analysis device 15 also includes a centralized power supply 151, a pump 152, a water outlet pipe 153, a total nitrogen probe 154, a nitrate nitrogen probe 155, and a water temperature probe 156. The centralized power supply 151 provides centralized power supply for the device. The pore water sampling tube 14 is connected to the pore water sampling bottle 157 through the pump 152 and the water outlet pipe 153 in sequence. During extraction, the stratified pore water enters the pore water sampling bottle 157 through the water outlet pipe 153. The total nitrogen probe 154, the nitrate nitrogen probe 155, and the water temperature probe 156 are set on the side of the pore water sampling bottle 157 for real-time analysis of key indicators affecting the stratified denitrification rate.

[0045] The apparatus for in-situ stratified multi-method determination of denitrification rate in beach land further includes a control panel 16 , which includes a signal receiver 161 and a display screen 162 . The signal receiver 161 is communicatively connected to the total nitrogen probe 154 , the nitrate nitrogen probe 155 , and the water temperature probe 156 , for displaying the analysis results of the indicators.

[0046] The control panel 16 also includes a culture chamber control button 163 and a direction button 164. The culture chamber control button 163 and / or the direction button 164 are communicatively connected to the piston drive motor 911 and / or the chamber door drive motor 711 for controlling the gas sampling chamber and the culture chamber injection device 9 of the device.

[0047] Working process: When it is necessary to carry out an in-situ denitrification rate experiment on a vertically layered beach and simultaneously obtain physical and chemical index data that match the denitrification rate, each culture chamber 6 corresponds to two gas sampling chambers, one is the initial gas sampling chamber 7, and the other is the termination gas sampling chamber 8. In the first step, the operator can select the length of the column 2 according to the sampling depth; in the second step, the operator pre-selects acetylene gas or isotope tracer to be loaded into the gas-liquid chamber 94 of the culture chamber injection device 9 according to the requirements of the denitrification experimental method (acetylene inhibition method, isotope tracer method, etc.); in the third step, the operator can push the fixed depth push rod 1 to vertically penetrate the experimental device into the beach, so that the interior of the column 2 is filled with the beach mud sample; in the fourth step, relying on the cohesion of the mud sample in the column 2, the operator slowly pushes the column 2 Slowly pull it out, and then insert the layered plug 17 into the column 2 along the layered seam 4 and the layered plug track 3 on the left side of the column 2 to realize the formation of a cubic culture chamber 6 with the upper and lower layers of layered plugs 17 and the side wall of the column 2; the fourth step is to slowly insert the layered column 2 into the in-situ hole of the beach again through the fixed depth push rod 1 to meet the temperature, humidity, low oxygen and other conditions of the in-situ experiment; the fifth step is to remotely control the culture chamber injection device 9 on the culture chamber 6 through the control panel 16 to start, and inject acetylene gas or isotope tracer into the layered culture chamber 6; the sixth step is that the initial gas sampling chamber 7 on the culture chamber 6 (the initial internal state is vacuum) is opened under the action of the chamber door drive motor 711, and the sliding door of the initial gas sampling chamber 7 is opened. The sliding door 75 is opened, and after the initial gas (nitrous oxide and nitrogen) enters the culture chamber 6, the sliding door 75 is closed. In the seventh step, the in-situ denitrification experiment in the stratified culture chamber 6 is waited for to reach the preset experimental time (generally 24 hours). In the eighth step, the sliding door of the termination gas sampling chamber 8 (the internal initial state is vacuum, and the structure is the same as that of the initial gas sampling chamber 7) on the culture chamber 6 is opened by the door drive motor. After the termination gas in the culture chamber 6 enters, the sliding door is closed, completing the sampling of the termination gas (nitrous oxide and nitrogen). In the ninth step, the operator turns on the pump 151 to extract the stratified pore water, and places the extracted pore water in the pore water sampling bottle 157 through the pore water sampling tube 14. and analyze the total nitrogen, nitrate nitrogen and water temperature indicators through the total nitrogen probe 154, the nitrate nitrogen probe 155 and the water temperature probe 156; in the tenth step, the operator slowly pulls the column 2 to the surface by pulling the fixed depth push rod 1 upward; in the eleventh step, the operator opens the lock 10 of the column 2, takes out mud samples of different depths, puts them into sample bottles, and cools them with liquid nitrogen for storage; in the twelfth step, the operator uses a 15ml syringe to extract the gas samples in the initial gas sampling chamber 7 and the termination gas sampling chamber 8 of the denitrification experiment in layers, and puts them into vacuum bottles for testing; in the thirteenth step, the operator brings the collected in-situ gas samples, mud samples and pore water samples back to the laboratory to carry out detection of gas sample concentration (for calculating denitrification rate), physical and chemical indicators and microbial abundance.In summary, the structure of this device has the advantage of modular combination and wider application scenarios. It solves the current problem of difficulty in conducting in situ denitrification rate experiments in vertical stratification on beaches, and meets the needs of in situ stratification determination of denitrification rates by various methods such as acetylene inhibition method and isotope tracer method. At the same time, it also has the functions of synchronous analysis of the main indicators affecting the denitrification rate (total nitrogen, nitrate nitrogen, temperature) and mud sample collection (for subsequent determination of denitrification microbial structure and abundance), achieving a good match between in situ denitrification rate and in situ physical and chemical data, meeting the needs of in-depth analysis of the influencing mechanism of in situ stratified denitrification rate, and providing guidance and data reference for scientifically measuring the denitrification contribution within the beach and evaluating the ecological value of the beach, and has high promotion and application value.

[0048] The above description is merely a specific embodiment of the present invention, and the scope of protection of the present invention is not limited thereto. Any simple modification or substitution made by a person skilled in the art within the technical scope disclosed in the present invention should be covered by the scope of protection of the present invention. Therefore, the present application is not limited to the specific embodiments described herein, but is intended to cover the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A device for determining the denitrification rate of beach in-situ stratification by multiple methods, characterized in that: It comprises a column (2), a gas sampling chamber and a culture chamber injection device (9), The column (2) is a vertical square column with an open bottom and a hollow interior. The lower ends of the front side and rear side of the column (2) are fixedly connected to a triangular plate (5) with a downwardly pointed tip; a plurality of layered slits (4) are provided on the left side of the column (2); layered plug-in plate rails (3) are provided on the inner sides of the front side and rear side corresponding to the layered slits (4); a layered plug-in plate (17) is provided in conjunction with each pair of the layered plug-in plate rails (3); the layered plug-in plates (17) pass through the layered slits (4); The layered insert (17) divides the internal space of the column (2) into a plurality of closed culture chambers (6). Two gas sampling chambers and a culture chamber injection device (9) are respectively arranged on the front side corresponding to each of the culture chambers (6). The right side surface of the column (2) is hinged to the front side surface or the rear side surface.

2. The device according to claim 1, characterized in that A depth-fixing push rod (1) is fixedly connected to the top of the column (2).

3. The device according to claim 1, characterized in that The gas sampling chamber comprises a gas chamber (73), a gas permeable membrane (74), a sliding chamber door (75) and a sealing ring (76). The port of the gas chamber (73) is closed by a breathable membrane (74), and a sealing ring (76) is provided between the port of the gas chamber (73) and the breathable membrane (74). The cross section of the sealing ring (76) is a U-shape with the opening facing the heart. The top of the sealing ring (76) has an opening that cooperates with the sliding chamber door (75). When the sliding chamber door (75) is in an open state, the gas chamber (73) is connected to the culture chamber (6) through the inner ring of the sealing ring (76). The gas chamber (73) is fixedly connected to the front side.

4. The device according to claim 3, characterized in that The gas sampling chamber further comprises a first pusher (71) and a chamber door push rod (72); the first pusher (71) comprises a chamber door transmission motor (711), a chamber door transmission gear (712), and a chamber door screw (713); The door screw (713) and the door push rod (72) are rotatably connected via a thread, the door transmission motor (711) is connected to the door screw (713) via the door transmission gear (712), and the door push rod (72) is fixedly connected to the top end of the sliding door (75) to realize opening or closing of the sliding door (75).

5. The device according to claim 1, characterized in that The culture chamber injection device (9) comprises a gas-liquid chamber (94) and a piston (93) for compressing the gas-liquid chamber (94); the gas-liquid chamber (94) is connected to the interior of the culture chamber (6) via an injection needle (95).

6. The device according to claim 5, characterized in that The culture chamber injection device (9) further comprises a second pusher (91) and a piston push rod (92); the second pusher (91) comprises a piston transmission motor (911), a piston transmission gear (912), and a piston screw (913). The piston drive motor (911) is connected to the piston screw (913) via the piston drive gear (912); the piston screw (913) and the piston push rod (92) are rotationally connected via a thread; the piston push rod (92) is fixedly connected to the piston (93), thereby enabling the piston (93) to compress the gas-liquid tank (94).

7. The device according to claim 1, characterized in that The device for determining the denitrification rate of beach in-situ stratification by multiple methods is also provided with a pore water collection and analysis system. The pore water sampling and analysis system comprises a pore water filter membrane (11), a pore water sampling hole (12), a pore water sampling housing (13), a pore water sampling tube (14), and a pore water collection and analysis device (15). The pore water collection and analysis device (15) comprises a pore water sampling bottle (157). The pore water sampling hole (12) is arranged in the middle of the pore water sampling housing (13); the pore water sampling hole (12) is connected to one end of the pore water sampling tube (14) through the pore water filter membrane (11); the other end of the pore water sampling tube (14) is connected to the pore water sampling bottle (157). The pore water sampling housing (13) is fixed to the right side surface of the column (2).

8. The device according to claim 7, characterized in that The pore water collection and analysis device (15) also includes a centralized power supply (151), a pump (152), a water outlet pipe (153), a total nitrogen probe (154), a nitrate nitrogen probe (155), a water temperature probe (156), The pore water sampling tube (14) is connected to the pore water sampling bottle (157) through the pump (152) and the water outlet pipe (153) in sequence. The total nitrogen probe (154), the nitrate nitrogen probe (155) and the water temperature probe (156) are arranged on the side of the pore water sampling bottle (157). The centralized power source (151) supplies power to the pore water collection and analysis device (15).

9. The device according to claim 8, characterized in that The device for determining the denitrification rate of beach in-situ stratified multi-methods further comprises a control panel (16), wherein the control panel (16) comprises a signal receiver (161) and a display screen (162). The signal receiver (161) is communicatively connected to the total nitrogen probe (154), the nitrate nitrogen probe (155), and the water temperature probe (156).

10. The device according to claim 1, characterized in that The right side is locked by a column lock (10).

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