A device for determining denitrification rate by multi-method in-situ stratification in beach land

Abstract

The present invention discloses a device for determining the denitrification rate of a beach by in-situ stratification and multiple methods, comprising a vertical square column body with an opening at the bottom and a hollow interior, wherein the lower ends of the front side and the rear side are fixedly connected to a triangular plate with the tip facing downward, a plurality of stratification seams are arranged on the left side, and a stratification plug-in plate track is arranged on the inner side corresponding to the stratification seams, and a stratification plug-in plate is arranged to divide the internal space of the column body into a plurality of closed culture bins in cooperation with the stratification plug-in plate track, and each culture bin is respectively provided with two gas sampling bins and a culture bin injection device. The device can realize in-situ stratification to carry out denitrification rate experiments, and can simultaneously meet the needs of two denitrification experimental methods, the acetylene inhibition method and the isotope tracer method, and can meet the needs of determining the structure and abundance of denitrification microorganisms, and can achieve a good matching degree between the in-situ denitrification rate and the in-situ physical and chemical data, and has a high practical value and promotion prospect for understanding and mastering the internal nitrogen cycle process of the beach and evaluating the ecological and environmental benefits of the beach.

Description

Technical Field

[0001] The invention belongs to the field of beach environment monitoring, and in particular relates to a device for determining denitrification rate of a beach by in-situ stratification and multiple methods. Background Art

[0002] Beaches are key connecting zones between land and water (river and lake shores, coastal beaches), and are sensitive areas where hydrological, chemical, and ecological processes interact in aquatic ecosystems. They have a high abundance of denitrifying and anaerobic ammonium oxidation (ANAMMOX) denitrification microorganisms. It is reported that denitrification and ANAMMOX (contributing up to 37.5-67.6%) dominate the denitrification of saturated aquifers and unsaturated soil layers, respectively (Zhu et al, Scientific Reports, 209), and play an important role in maintaining the nitrogen balance of regional and even global aquatic ecosystems.

[0003] However, current research on the denitrification process of beaches often uses mud samples (including pore water samples) collected in situ and brought back to the laboratory to conduct indoor denitrification rate measurement experiments, and adopts acetylene inhibition method or isotope tracer method according to the experimental objectives. However, there are two main problems with indoor experiments. On the one hand, the physical and chemical indicators and redox conditions of the mud samples (including pore water samples) collected in situ have undergone significant changes. On the other hand, the abundance and structure of denitrification functional microorganisms in the samples may also change significantly due to changes in environmental conditions. The experimental results are difficult to truly reflect the in situ denitrification conditions and actual denitrification rates, and it is difficult to provide guidance and reference for scientifically measuring the denitrification contribution within the beach and evaluating the corresponding ecological value. Summary of the invention

[0004] In view of the above-mentioned problems of the prior art, the present invention designs a device for in-situ stratified multi-method determination of denitrification rate in beaches, so as to solve the current difficulty in conducting in-situ stratified multi-method determination of denitrification rate experiments in beaches.

[0005] The device can realize in-situ stratified denitrification rate experiments, and can simultaneously meet the needs of two denitrification experimental methods: acetylene inhibition method and isotope tracer method. In addition, it also has the functions of synchronously analyzing the main indicators affecting the denitrification rate (total nitrogen, nitrate nitrogen, temperature) and collecting mud samples (for subsequent determination of denitrification microbial structure and abundance), which can achieve a good match between the in-situ denitrification rate and the in-situ physical and chemical data, and provide guidance and data reference for scientifically measuring the denitrification contribution and impact mechanism within the beach, as well as evaluating the ecological value of the beach, and meet the needs of in-depth analysis of the impact mechanism of in-situ stratified denitrification rate.

[0006] In order to achieve the above object, the technical solution adopted by the present invention is as follows:

[0007] A device for determining the denitrification rate of a beach in situ by multiple methods using stratified methods, comprising a column 2, a gas sampling chamber and a culture chamber injection device 9,

[0008] The column 2 is a vertical square column with an open bottom and a hollow interior.

[0009] The lower ends of the front side and the rear side of the column 2 are fixedly connected to a triangular plate 5 with the tip pointing downward. A plurality of layered seams 4 are arranged on the left side of the column 2. Layered plug-in board tracks 3 are arranged on the inner sides of the front side and the rear side corresponding to the layered seams 4. A layered plug-in board 17 is arranged to match each pair of the layered plug-in board tracks 3. The layered plug-in board 17 passes through the layered seams 4.

[0010] The layered insert 17 divides the internal space of the column 2 into a plurality of closed culture chambers 6.

[0011] Two gas sampling chambers and a culture chamber injection device 9 are respectively arranged on the front side corresponding to each culture chamber 6.

[0012] The right side surface of the column 2 is hinged to the front side surface or the rear side surface.

[0013] The triangular plate 5 is used for piercing into the soft beach.

[0014] The top of the column 2 is fixedly connected with a depth-fixing push rod 1, which can assist in pushing the column 2 into the mud of the beach.

[0015] The right side can be opened after the experiment is completed.

[0016] The right side is locked by a cylinder lock 10.

[0017] The gas sampling chamber includes a gas chamber 73, a breathable membrane 74, a sliding chamber door 75, and a sealing ring 76. The chamber opening of the gas chamber 73 is closed by the breathable membrane 74, and a sealing ring 76 is arranged between the chamber opening of the gas chamber 73 and the breathable membrane 74. The cross-section of the sealing ring 76 is a U-shape with an opening concentrically facing the chamber. The top of the sealing ring 76 has an opening that cooperates with the sliding chamber door 75. When the sliding chamber door 75 is in the open state, the gas chamber 73 is connected to the culture chamber 6 through the inner ring of the sealing ring 76, and the gas chamber 73 is fixedly connected to the front side of the column 2.

[0018] The gas sampling chamber further includes a first pusher 71 and a chamber door push rod 72. The first pusher 71 includes a chamber door transmission motor 711, a chamber door transmission gear 712, and a chamber door screw 713.

[0019] The door screw 713 and the door push rod 72 are rotationally connected via a thread, the door transmission motor 711 is connected to the door screw 713 via the door transmission gear 712, and the door push rod 72 is fixedly connected to the top of the sliding door 75 to realize the opening or closing of the sliding door 75.

[0020] The initial state of the gas bin 73 is vacuum, and the sliding bin door 75 can be opened and closed up and down under the pushing and pulling action of the first pusher 71 and the bin door push rod 72. The breathable membrane 74 can prevent the mud sample from entering and allow the gas in the culture bin 6 to enter the gas bin 73. The sealing ring 76 can ensure that the gas bin 73 is airtight when the sliding bin door 75 is closed. The gas bin 73 is fixed on the front side of the column 2 and is used to collect nitrous oxide and nitrogen produced in the experiment in the culture bin 6.

[0021] The culture chamber injection device 9 includes a gas-liquid chamber 94 and a piston 93 for compressing the gas-liquid chamber 94 . The gas-liquid chamber 94 is connected to the interior of the culture chamber 6 through an injection needle 95 .

[0022] The culture chamber injection device 9 also includes a second pusher 91 and a piston push rod 92. The second pusher 91 includes a piston transmission motor 911, a piston transmission gear 912, and a piston screw 913. The piston transmission motor 911 is connected to the piston screw 913 via the piston transmission gear 912. The piston screw 913 and the piston push rod 92 are rotationally connected via a thread. The piston push rod 92 is fixedly connected to the piston 93 to achieve compression of the gas-liquid chamber 94 by the piston 93.

[0023] The second pusher 91 pushes the piston push rod 92 and the piston 93 to inject the acetylene gas or isotope tracer in the gas-liquid tank 94 into the culture tank 6 .

[0024] The device for in-situ stratified multi-method determination of denitrification rate in beach is also provided with a pore water collection and analysis system, including a pore water filter membrane 11, a pore water sampling hole 12, a pore water sampling shell 13, a pore water sampling tube 14, and a pore water collection and analysis device 15. The pore water collection and analysis device 15 includes a pore water sampling bottle 157. The pore water sampling hole 12 is arranged in the middle of the pore water sampling shell 13. The pore water sampling hole 12 is connected to one end of the pore water sampling tube 14 through the pore water filter membrane 11. The other end of the pore water sampling tube 14 is connected to the pore water sampling bottle 157. The pore water sampling shell 13 is fixed to the right side of the column 2.

[0025] The pore water collection and analysis device 15 also includes a centralized power supply 151, a pump 152, a water outlet pipe 153, a total nitrogen probe 154, a nitrate nitrogen probe 155, and a water temperature probe 156. The centralized power supply 151 is used to centrally power the device. The pore water sampling tube (14) is connected to the pore water sampling bottle 157 through the pump 152 and the water outlet pipe 153 in sequence. When extracting, the stratified pore water enters the pore water sampling bottle 157 through the water outlet pipe 153. The total nitrogen probe 154, the nitrate nitrogen probe 155, and the water temperature probe 156 are arranged on the side of the pore water sampling bottle 157 for real-time analysis of key indicators affecting the stratified denitrification rate.

[0026] The device for determining the denitrification rate of beach in-situ stratified multi-methods also includes a control panel 16, which includes a signal receiver 161 and a display screen 162. The signal receiver 161 is connected to the total nitrogen probe 154, the nitrate nitrogen probe 155 and the water temperature probe 156 for displaying the analysis results of the indicators.

[0027] The control panel 16 also includes a culture chamber control button 163 and a direction button 164. The culture chamber control button 163 and / or the direction button 164 are communicatively connected with the piston drive motor 911 and / or the chamber door drive motor 711, and are used to control the gas sampling chamber and the culture chamber injection device 9 of the device.

[0028] During the experiment, each culture chamber 6 corresponds to two gas sampling chambers, one is the initial gas sampling chamber 7, and the other is the termination gas sampling chamber 8. The operator can pre-select to load acetylene gas or isotope tracer into the gas-liquid chamber 94 of the culture chamber injection device 9 according to the requirements of the in-situ denitrification experimental method, and make the gas chamber 73 vacuum and close the sliding chamber door 75. After completing the above-mentioned experimental equipment layout, the operator pushes the fixed depth push rod 1 to vertically penetrate the experimental device into the beach, so that the inside of the column 2 is filled with beach mud samples, and then the operator slowly pulls out the column 2 vertically, and inserts the layered plug 17 into the column 2 along the layered seam 4 and the layered plug track 3 on the left side of the column 2, so that the upper and lower layers of the layered plug 17 and the side wall of the column 2 form a cubic culture chamber. The layered column 2 is slowly penetrated into the in-situ hole of the beach again through the fixed depth push rod 1 to meet the temperature, humidity, hypoxia and other conditions required for the in-situ experiment. The culture chamber injection device 9 on the culture chamber 6 is remotely controlled through the control panel 16 to start, and the acetylene gas or isotope tracer in the gas-liquid chamber 94 is injected into the layered culture chamber 6. The initial gas sampling chamber 7 on the culture chamber 6 (the initial state inside is vacuum) opens the sliding chamber door 75 of the initial gas sampling chamber 7 under the action of the chamber door transmission motor 711, and closes the sliding chamber door 75 after the initial gas (nitrous oxide and nitrogen) in the culture chamber 6 enters. When the in-situ denitrification experiment in the layered culture chamber 6 reaches the preset experimental duration (generally 24 hours), the termination gas sampling chamber 8 on the culture chamber 6 (the initial state inside is vacuum, and the structure is the same as that of the initial gas sampling chamber 7) is remotely controlled to open the sliding chamber door of the termination gas sampling chamber 8 under the action of the chamber door transmission motor, and closes it after the termination gas in the culture chamber 6 enters, completing the sampling of the termination gas (nitrous oxide and nitrogen). At the same time as the in-situ denitrification experiment is completed, the pump 151 is turned on to extract the stratified pore water, and the extracted pore water is placed in the pore water sampling bottle 157 through the pore water sampling tube 14, and the total nitrogen, nitrate nitrogen and water temperature indicators are analyzed through the total nitrogen probe 154, the nitrate nitrogen probe 155 and the water temperature probe 156. After the in-situ denitrification experiment and pore water detection are completed, the operator pulls the fixed depth push rod 1 upward, slowly pulls the column 2 to the surface, opens the lock 10 of the column 2, takes out the mud samples of different depths, puts them into the sample bottle, and cools them with liquid nitrogen for storage. In addition, a 15ml syringe is used to extract the gas samples in the initial gas sampling chamber 7 and the termination gas sampling chamber 8 of the denitrification experiment in layers, and puts them into a vacuum bottle for testing. Finally, the collected in-situ gas samples, mud samples and pore water samples are brought back to the laboratory for further testing of gas sample concentration (used to calculate the denitrification rate), physical and chemical indicators and microbial abundance.

[0029] On the whole, the structure of this device has the advantage of modular combination and wider application scenarios. It solves the current problem of difficulty in conducting in situ denitrification rate experiments on vertical stratification in beaches, and meets the needs of in situ stratified determination of denitrification rates by various methods such as acetylene inhibition method and isotope tracer method. At the same time, it also has the function of synchronously analyzing the main indicators affecting the denitrification rate (total nitrogen, nitrate nitrogen, temperature) and mud sample collection (for subsequent determination of denitrification microbial structure and abundance), achieving a good match between the in situ denitrification rate and the in situ physical and chemical data, meeting the needs of in-depth analysis of the influencing mechanism of the in situ stratified denitrification rate, and providing guidance and data reference for scientifically measuring the denitrification contribution within the beach and evaluating the ecological value of the beach, and has a high value for promotion and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] The drawings in the specification, which constitute a part of this application, are used to provide a further understanding of the present invention. The illustrative embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0031] Figure 1 It is a schematic diagram of the structure of a device for in-situ stratified multi-method determination of denitrification rate in a beach in Example 1 of the present invention.

[0032] Figure 2 It is a schematic diagram of the three-dimensional structure of the column in Example 1.

[0033] Figure 3 It is a schematic diagram of the structure of the initial gas sampling chamber in Example 1 (the termination gas sampling chamber has the same structure), wherein the left figure is a schematic diagram of the structure of the sliding chamber door in a closed state, and the right figure is a schematic diagram of the structure of the sliding chamber door in an open state.

[0034] Figure 4 It is a schematic diagram of the structure of the first pusher in Example 1.

[0035] Figure 5 It is a schematic diagram of the structure of the culture chamber injection device in Example 1.

[0036] Figure 6 It is a schematic diagram of the structure of the second pusher in Example 1.

[0037] Among them, 1 is a fixed depth push rod, 2 is a column, 3 is a layered plug track, 4 is a layered seam, 5 is a triangular plate, 6 is a culture chamber, 7 is an initial gas sampling chamber, 71 is a first pusher, 72 is a chamber door push rod, 73 is a gas chamber, 74 is a breathable membrane, 75 is a sliding chamber door, 76 is a sealing ring, 711 is a chamber door drive motor, 712 is a chamber door drive gear, 713 is a chamber door screw, 8 is a termination gas sampling chamber, 9 is a culture chamber injection device, 91 is a second pusher, 92 is a piston push rod, 93 is a piston, 94 is a gas-liquid chamber, 95 is an injection needle, 911 is a piston drive motor, 912 is the piston transmission gear, 913 is the piston screw, 10 is the column lock, 11 is the pore water filter membrane, 12 is the pore water sampling hole, 13 is the pore water sampling shell, 14 is the pore water sampling tube, 15 is the pore water collection and analysis device, 151 is the centralized power supply, 152 is the pump, 153 is the outlet pipe, 154 is the total nitrogen probe, 155 is the nitrate nitrogen probe, 156 is the water temperature probe, 157 is the pore water sampling bottle, 16 is the control panel, 161 is the signal receiver, 162 is the display screen, 163 is the culture chamber control button, 164 is the direction button, and 17 is the layered plug board. DETAILED DESCRIPTION

[0038] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and in combination with the embodiments. Example 1

[0039] like Figures 1 to 6 The device shown is used for in-situ stratified multi-method determination of denitrification rate in beach land, comprising a column 2, an initial gas sampling chamber 7, a final gas sampling chamber 8 and a culture chamber injection device 9.

[0040] The column 2 is a vertical square column with an open bottom and a hollow interior.

[0041] The lower ends of the front side and the rear side of the column 2 are fixedly connected to a triangular plate 5 with the tip pointing downward, a plurality of layered seams 4 are arranged on the left side of the column 2, and layered plug-in board tracks 3 are arranged on the inner sides of the front side and the rear side of the column 2 respectively corresponding to the layered seams 4, and a layered plug-in board 17 is arranged to match each pair of the layered plug-in board tracks 3, and the layered plug-in board 17 passes through the layered seams 4.

[0042] The layered insert 17 divides the internal space of the column 2 into a plurality of closed culture chambers 6.

[0043] Two gas sampling chambers and a culture chamber injection device 9 are respectively arranged on the front side corresponding to each culture chamber 6.

[0044] The right side surface of the column 2 is hinged to the front side surface or the rear side surface.

[0045] The triangular plate 5 is used for piercing into the soft beach.

[0046] The top of the column 2 is fixedly connected with a depth-fixing push rod 1, which can assist in pushing the column 2 into the mud of the beach.

[0047] The right side can be opened after the experiment is completed.

[0048] The right side is locked by a cylinder lock 10.

[0049] The gas sampling chamber includes a gas chamber 73, a breathable membrane 74, a sliding chamber door 75, and a sealing ring 76. The chamber opening of the gas chamber 73 is closed by the breathable membrane 74, and a sealing ring 76 is arranged between the chamber opening of the gas chamber 73 and the breathable membrane 74. The cross-section of the sealing ring 76 is a U-shape with an opening facing the centripetal. The top of the sealing ring 76 has an opening that cooperates with the sliding chamber door 75. When the sliding chamber door 75 is in the open state, the gas chamber 73 is connected to the culture chamber 6 through the inner ring of the sealing ring 76, and the gas chamber 73 is fixedly connected to the front side.

[0050] The gas sampling chamber also includes a first pusher 71 and a chamber door push rod 72. The first pusher 71 includes a chamber door transmission motor 711, a chamber door transmission gear 712, and a chamber door screw 713. The chamber door screw 713 and the chamber door push rod 72 are rotationally connected by threads. The chamber door transmission motor 711 is connected to the chamber door screw 713 through the chamber door transmission gear 712. The chamber door push rod 72 is fixedly connected to the top of the sliding chamber door 75 to realize the opening or closing of the sliding chamber door 75.

[0051] The initial state of the gas bin 73 is vacuum, and the sliding bin door 75 can be opened and closed up and down under the pushing and pulling action of the first pusher 71 and the bin door push rod 72. The breathable membrane 74 can prevent the mud sample from entering and allow the gas in the culture bin 6 to enter the gas bin 73. The sealing ring 76 can ensure that the gas bin 73 is airtight when the sliding bin door 75 is closed. The gas bin 73 is fixed on the front side of the column 2 and is used to collect nitrous oxide and nitrogen produced in the experiment in the culture bin 6.

[0052] The culture chamber injection device 9 includes a gas-liquid chamber 94 and a piston 93 for compressing the gas-liquid chamber 94 . The gas-liquid chamber 94 is connected to the interior of the culture chamber 6 through an injection needle 95 .

[0053] The culture chamber injection device 9 also includes a second pusher 91 and a piston push rod 92. The second pusher 91 includes a piston transmission motor 911, a piston transmission gear 912, and a piston screw 913. The piston transmission motor 911 is connected to the piston screw 913 via the piston transmission gear 912. The piston screw 913 and the piston push rod 92 are rotationally connected via a thread. The piston push rod 92 is fixedly connected to the piston 93 to achieve compression of the gas-liquid chamber 94 by the piston 93.

[0054] The second pusher 91 pushes the piston push rod 92 and the piston 93 to inject the acetylene gas or isotope tracer in the gas-liquid tank 94 into the culture tank 6 .

[0055] The device for in-situ stratified multi-method determination of denitrification rate in beach is also provided with a pore water collection and analysis system, including a pore water filter membrane 11, a pore water sampling hole 12, a pore water sampling shell 13, a pore water sampling tube 14, and a pore water collection and analysis device 15. The pore water collection and analysis device 15 includes a pore water sampling bottle 157. The pore water sampling hole 12 is arranged in the middle of the pore water sampling shell 13. The pore water sampling hole 12 is connected to one end of the pore water sampling tube 14 through the pore water filter membrane 11. The other end of the pore water sampling tube 14 is connected to the pore water sampling bottle 157. The pore water sampling shell 13 is fixed to the right side of the column 2.

[0056] The pore water collection and analysis device 15 also includes a centralized power supply 151, a pump 152, a water outlet pipe 153, a total nitrogen probe 154, a nitrate nitrogen probe 155, and a water temperature probe 156. The centralized power supply 151 is used to centrally power the device. The pore water sampling tube 14 is connected to the pore water sampling bottle 157 through the pump 152 and the water outlet pipe 153 in sequence. During extraction, the stratified pore water enters the pore water sampling bottle 157 through the water outlet pipe 153. The total nitrogen probe 154, the nitrate nitrogen probe 155, and the water temperature probe 156 are arranged on the side of the pore water sampling bottle 157 for real-time analysis of key indicators affecting the stratified denitrification rate.

[0057] The device for determining the denitrification rate of beach in-situ stratified multi-methods also includes a control panel 16, which includes a signal receiver 161 and a display screen 162. The signal receiver 161 is connected to the total nitrogen probe 154, the nitrate nitrogen probe 155 and the water temperature probe 156 for displaying the analysis results of the indicators.

[0058] The control panel 16 also includes a culture chamber control button 163 and a direction button 164. The culture chamber control button 163 and / or the direction button 164 are communicatively connected with the piston drive motor 911 and / or the chamber door drive motor 711, and are used to control the gas sampling chamber and the culture chamber injection device 9 of the device.

[0059] Working process:

[0060] When it is necessary to carry out an in-situ denitrification rate experiment on a beach vertically layered and simultaneously obtain physical and chemical index data that match the denitrification rate, each culture chamber 6 corresponds to two gas sampling chambers, one is an initial gas sampling chamber 7, and the other is a termination gas sampling chamber 8. In the first step, the operator can select the length of the column 2 according to the sampling depth; in the second step, the operator pre-selects acetylene gas or isotope tracer to be loaded into the gas-liquid chamber 94 of the culture chamber injection device 9 according to the requirements of the denitrification experimental method (acetylene inhibition method, isotope tracer method, etc.); in the third step, the operator can push the fixed depth push rod 1 to vertically penetrate the experimental device into the beach, so that the interior of the column 2 is filled with beach mud samples; in the fourth step, relying on the cohesion of the mud sample in the column 2, the operator slowly pushes the column 2. Slowly pull it out, and then insert the layered plug board 17 into the column 2 along the layered seam 4 and the layered plug board track 3 on the left side of the column 2, so that the upper and lower layers of the layered plug boards 17 and the side walls of the column 2 form a cubic culture chamber 6; the fourth step is to slowly insert the layered column 2 into the in-situ hole of the beach again through the fixed depth push rod 1 to meet the temperature, humidity, hypoxia and other conditions of the in-situ experiment; the fifth step is to remotely control the culture chamber injection device 9 on the culture chamber 6 through the control panel 16 to start, and inject acetylene gas or isotope tracer into the layered culture chamber 6; the sixth step is that the initial gas sampling chamber 7 on the culture chamber 6 (the initial state inside is vacuum) opens the sliding door of the initial gas sampling chamber 7 under the action of the chamber door transmission motor 711 The sliding door 75 is moved, and after the initial gas (nitrous oxide and nitrogen) enters the culture chamber 6, the sliding door 75 is closed; the seventh step is to wait for the in-situ denitrification experiment in the stratified culture chamber 6 to reach the preset experiment time (generally 24 hours); the eighth step is that the end gas sampling chamber 8 (the internal initial state is vacuum, and the structure is the same as the initial gas sampling chamber 7) on the culture chamber 6 opens the sliding door of the end gas sampling chamber 8 under the action of the door drive motor, and after the end gas in the culture chamber 6 enters, it is closed again to complete the sampling of the end gas (nitrous oxide and nitrogen); the ninth step is that the operator turns on the pump 151 to extract the stratified pore water, and the extracted pore water is placed in the pore water sampling bottle 157 through the pore water sampling tube 14. In the tenth step, the operator slowly pulls the column 2 to the surface by pulling the fixed depth push rod 1 upwards; in the eleventh step, the operator opens the lock 10 of the column 2, takes out mud samples of different depths, puts them into sample bottles, and cools and preserves them with liquid nitrogen; in the twelfth step, the operator uses a 15ml syringe to extract gas samples in the initial gas sampling chamber 7 and the termination gas sampling chamber 8 of the denitrification experiment in layers, and puts them into vacuum bottles for testing; in the thirteenth step, the operator takes the collected in-situ gas samples, mud samples and pore water samples back to the laboratory to carry out detection of gas sample concentration (for calculating denitrification rate), physical and chemical indicators and microbial abundance.On the whole, the structure of this device has the advantage of modular combination and wider application scenarios. It solves the current problem of difficulty in conducting in situ denitrification rate experiments on vertical stratification in beaches, and meets the needs of in situ stratified determination of denitrification rates by various methods such as acetylene inhibition method and isotope tracer method. At the same time, it also has the function of synchronously analyzing the main indicators affecting the denitrification rate (total nitrogen, nitrate nitrogen, temperature) and mud sample collection (for subsequent determination of denitrification microbial structure and abundance), achieving a good match between the in situ denitrification rate and the in situ physical and chemical data, meeting the needs of in-depth analysis of the influencing mechanism of the in situ stratified denitrification rate, and providing guidance and data reference for scientifically measuring the denitrification contribution within the beach and evaluating the ecological value of the beach, and has a high value for promotion and application.

[0061] The above description is only a specific implementation of the present invention, and the protection scope of the present invention is not limited thereto. Any simple transformation or replacement made by a person skilled in the art within the technical scope disclosed by the present invention should be included in the protection scope of the present invention. Therefore, the present application will not be limited to the specific embodiments described herein, but can cover the widest range consistent with the principles and novel features disclosed herein.

Claims

1. A device for determining the denitrification rate of beach in-situ stratification by multiple methods, characterized in that: It comprises a column (2), a gas sampling chamber and a culture chamber injection device (9), The column (2) is a vertical square column with an open bottom and a hollow interior. The lower ends of the front side and rear side of the column (2) are fixedly connected to a triangular plate (5) with a downwardly pointed tip; a plurality of layered slits (4) are provided on the left side of the column (2); layered plug-in plate rails (3) are provided on the inner sides of the front side and rear side corresponding to the layered slits (4); a layered plug-in plate (17) is provided in conjunction with each pair of the layered plug-in plate rails (3); the layered plug-in plates (17) pass through the layered slits (4); The layered insert (17) divides the internal space of the column (2) into a plurality of closed culture chambers (6). Two gas sampling chambers and a culture chamber injection device (9) are respectively arranged on the front side surface corresponding to each culture chamber (6), wherein one of the two gas sampling chambers is an initial gas sampling chamber (7) and the other is a termination gas sampling chamber (8). The gas sampling chamber comprises a gas chamber (73), a gas permeable membrane (74), a sliding chamber door (75) and a sealing ring (76). The port of the gas chamber (73) is closed by a breathable membrane (74), and a sealing ring (76) is provided between the port of the gas chamber (73) and the breathable membrane (74). The cross section of the sealing ring (76) is a U-shape with the opening facing the heart. The top of the sealing ring (76) has an opening that cooperates with the sliding chamber door (75). When the sliding chamber door (75) is in an open state, the gas chamber (73) is connected to the culture chamber (6) through the inner ring of the sealing ring (76). The gas chamber (73) is fixedly connected to the front side. The culture chamber injection device (9) comprises a gas-liquid chamber (94) and a piston (93) for compressing the gas-liquid chamber (94); the gas-liquid chamber (94) is connected to the interior of the culture chamber (6) via an injection needle (95). The right side surface of the column (2) is hinged to the front side surface or the rear side surface.

2. The device according to claim 1, characterized in that A depth-fixing push rod (1) is fixedly connected to the top of the column (2).

3. The device according to claim 1, characterized in that The gas sampling chamber further comprises a first pusher (71) and a chamber door push rod (72); the first pusher (71) comprises a chamber door transmission motor (711), a chamber door transmission gear (712), and a chamber door screw (713); The door screw (713) and the door push rod (72) are rotatably connected via a thread, the door transmission motor (711) is connected to the door screw (713) via the door transmission gear (712), and the door push rod (72) is fixedly connected to the top end of the sliding door (75) to realize opening or closing of the sliding door (75).

4. The device according to claim 1, characterized in that The culture chamber injection device (9) further comprises a second pusher (91) and a piston push rod (92); the second pusher (91) comprises a piston transmission motor (911), a piston transmission gear (912), and a piston screw (913). The piston drive motor (911) is connected to the piston screw (913) via the piston drive gear (912); the piston screw (913) and the piston push rod (92) are rotationally connected via a thread; the piston push rod (92) is fixedly connected to the piston (93), thereby enabling the piston (93) to compress the gas-liquid tank (94).

5. The device according to claim 1, characterized in that The device for determining the denitrification rate of beach in-situ stratification by multiple methods is also provided with a pore water collection and analysis system. The pore water sampling and analysis system comprises a pore water filter membrane (11), a pore water sampling hole (12), a pore water sampling housing (13), a pore water sampling tube (14), and a pore water collection and analysis device (15). The pore water collection and analysis device (15) comprises a pore water sampling bottle (157). The pore water sampling hole (12) is arranged in the middle of the pore water sampling housing (13); the pore water sampling hole (12) is connected to one end of the pore water sampling tube (14) through the pore water filter membrane (11); the other end of the pore water sampling tube (14) is connected to the pore water sampling bottle (157). The pore water sampling housing (13) is fixed to the right side surface of the column (2).

6. The device according to claim 5, characterized in that The pore water collection and analysis device (15) also includes a centralized power supply (151), a pump (152), a water outlet pipe (153), a total nitrogen probe (154), a nitrate nitrogen probe (155), a water temperature probe (156), The pore water sampling tube (14) is connected to the pore water sampling bottle (157) through the pump (152) and the water outlet pipe (153) in sequence. The total nitrogen probe (154), the nitrate nitrogen probe (155) and the water temperature probe (156) are arranged on the side of the pore water sampling bottle (157). The centralized power source (151) supplies power to the pore water collection and analysis device (15).

7. The device according to claim 6, characterized in that The device for determining the denitrification rate of beach in-situ stratified multi-methods further comprises a control panel (16), wherein the control panel (16) comprises a signal receiver (161) and a display screen (162). The signal receiver (161) is communicatively connected to the total nitrogen probe (154), the nitrate nitrogen probe (155), and the water temperature probe (156).

8. The device according to claim 1, characterized in that The right side is locked by a column lock (10).

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