Sperm and cell counting detection glass slide

Abstract

The invention provides a sperm and cell counting detection glass slide, which comprises a substrate and a plurality of glass slides, the substrate is provided with a plurality of accommodating grooves for insertion of the glass slides, one side of each accommodating groove is open, each glass slide comprises a hollow glass slide body, the height of a cavity in the glass slide body is 10-20 microns, and the height of the cavity in the glass slide body is 10-20 microns. A dropping liquid inlet connected with the cavity is formed in the top of the glass slide body, and a fixing mechanism capable of fixing the glass slide and assisting the glass slide in popping up is arranged on the substrate. During detection, an adaptive glass slide is selected, sperm preservation liquid is dropped from a liquid dropping inlet, the liquid uniformly enters the cavity by utilizing the siphon effect of the glass slide cavity, the sperms are not overlapped in the movement process due to the limitation of the height of the cavity, and the detection accuracy of the number, activity and the like of the sperms is improved. By means of the principle, tiling of thin-layer single cells is formed, and due to the fact that the height and cleanliness of the cavity are accurately controlled, the detection efficiency and accuracy are improved for conventional sperm detection.

Description

Technical Field

[0001] The invention relates to the technical field of glass slides, in particular to a glass slide for sperm and cell counting detection. Background Art

[0002] Semen analysis is the most basic test method for evaluating male fertility, among which sperm count is one of the most important indicators.

[0003] At present, the method mainly adopted in China is to drip animal sperm or cells and then cover the cover glass. For example, a sperm cell counting slide with Chinese patent publication number CN213041748U includes, from bottom to top, a slide, a support layer and a cover glass, the support layer is provided with at least one recessed portion containing a sample injection port, the cover glass is fixed on the support layer so that the recessed portion forms a counting cavity, and the sample injection port at least partially exposes the edge of the cover glass.

[0004] For example, the above-mentioned slides cannot control the height of the liquid level, which can easily cause sperm overlap and affect sperm motility. Therefore, it can only count sperm, but cannot fully detect sperm activity including important parameters such as survival time, and the process accuracy is difficult to control. Summary of the invention

[0005] 1. Technical issues to be resolved

[0006] In view of the deficiencies in the prior art, the present invention provides a sperm and cell counting detection slide, which solves the problems raised in the above-mentioned background technology.

[0007] (II) Technical solution

[0008] To achieve the above objectives, the present invention is implemented through the following technical solutions: a sperm and cell counting detection slide, including a substrate and a plurality of slides, the substrate is provided with a plurality of receiving grooves for the slides to be inserted, one side of the receiving grooves is open, the slide includes a hollow slide body, the chamber height in the slide body is 10 to 20 microns, the top of the slide body is provided with a drip inlet connected to the chamber, and the substrate is provided with a fixing mechanism that can fix the slide and the auxiliary slide to pop out.

[0009] Preferably, the fixing mechanism includes a T-shaped top block, a spring, and a rotating shaft. Slide grooves are provided on both sides of the accommodating groove. The top block is arranged on the side of the glass slide body and is slidably adapted in the slide groove. The spring is arranged in the slide groove and is arranged along its length direction. One end of the spring corresponds to the top block. The rotating shaft is rotatably connected in the substrate and is arranged along the length direction of a plurality of glass slides. One end of the spring extends outside the substrate. Magnetic block 1 and magnetic block 2 are provided on both sides of the rotating shaft. A side of the top block corresponding to the rotating shaft is a magnetic body. When magnetic block 1 faces the top block, the magnetic poles of the two are opposite, and when magnetic block 2 faces the top block, the magnetic poles of the two are the same.

[0010] Preferably, it also includes a conveying mechanism for assisting in delivering the dripping liquid into the dripping liquid inlet.

[0011] Preferably, the conveying mechanism includes a transverse movement component, a support plate, and a funnel. The transverse movement component is arranged on the substrate and can drive the support plate to move along several directions of the glass slides. The support plate is detachably connected to the transverse movement component. The funnel is arranged on the support plate and corresponds to the drip inlet up and down.

[0012] Preferably, the transverse movement assembly includes a slider, a rack, a gear, and a connecting screw. A slide rail for slidingly adapting the slider is opened on the side of the base plate. The rack is arranged in the base plate along the length direction of the slide rail. The gear is pivotally connected to the slider and meshes with the rack. A screw hole is provided at the top center of the gear. The upper section of the connecting screw is vertically slidably adapted on the support plate, and the lower section is threadedly connected to the screw hole of the gear. A slot is vertically opened on the slider, and an insert block that can be vertically inserted into the slot is provided on the support plate.

[0013] Preferably, a positioning groove is provided on the substrate corresponding to each glass slide, and a positioning column matched with the positioning groove is provided at the bottom of the support plate. When the positioning column slides into the positioning groove, the funnel corresponds to the dripping inlet up and down.

[0014] Preferably, the thickness of the slider and the end of the rotating shaft extending out of the substrate are both smaller than the thickness of the substrate.

[0015] (III) Beneficial effects

[0016] The present invention provides a sperm and cell counting detection slide, which has the following beneficial effects:

[0017] 1. The sperm and cell counting test slide replaces the traditional drop-liquid cover glass for microscopic detection. During the test, the appropriate slide is selected according to the size of the sperm. The sperm preservation liquid is dripped into the drop-liquid inlet, and the siphon effect of the slide chamber is used to make the liquid enter the chamber evenly. The height limit of the chamber determines that the sperm will not overlap during movement, which improves the accuracy of sperm count and activity detection. The above principle is used to form a thin layer of single cells. Due to the precise control of the chamber height and cleanliness, it not only improves the detection efficiency and accuracy of conventional sperm detection, but also saves the detection operation time.

[0018] 2. The sperm and cell counting test slide, when loading the slide, first rotate the magnetic block 1 to the side facing the top block through the rotating shaft, then slide the top block of the slide into the slide groove, the top block slides in the slide groove and squeezes the spring, when the slide is completely loaded into the receiving groove, the top block moves to the end of the slide groove and adsorbs on the magnetic block 1, which can prevent the stability of the slide during the detection process. When the detection is completed, rotate the rotating shaft to make the magnetic block 1 separate from the top block, and at the same time, turn the magnetic block 2 to face the top block, so that the top block loses its adsorption force, and at the same time, the top block is pushed out of the slide groove under the repulsion of the magnetic block 2. Under the joint action of the spring, at least half of the slide is pushed out of the receiving groove, which is convenient for taking out the slide. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is an axonometric view of the substrate and the glass slide of the present invention;

[0020] Figure 2 is a side cross-sectional view of the substrate and the glass slide of the present invention;

[0021] Figure 3 It is a schematic diagram of loading a glass slide into a substrate of the present invention;

[0022] Figure 4 Schematic diagram of the substrate and the glass slide in the detection state of the present invention.

[0023] In the figure: 1 substrate, 2 glass slide, 3 receiving groove, 4 slide groove, 5 top block, 6 spring, 7 rotating shaft, 8 magnetic block 1, 9 magnetic block 2, 10 support plate, 11 funnel, 12 slider, 13 rack, 14 gear, 15 connecting screw, 16 slot, 17 insert block, 18 positioning groove, 19 positioning column, 21 glass slide body, 22 chamber, 23 drip inlet. DETAILED DESCRIPTION

[0024] The embodiment of the present invention provides a sperm and cell counting detection slide, such as Figure 1-4As shown, it includes a substrate 1 and a plurality of glass slides 2. The substrate 1 is provided with a plurality of receiving grooves 3 for the glass slides 2 to be inserted. In this embodiment, a total of four receiving grooves 3 and glass slides 2 are provided. One side of the receiving groove 3 is open, and the glass slide 2 slides in from the open side of the receiving groove 3. The glass slide 2 includes a hollow glass slide body 21. Since the size of human sperm is about 10 microns, and that of boars, bulls, etc. is 20 microns, the height of the chamber 22 in the glass slide body 21 is 10 to 20 microns. The height of the chamber 22 in different glass slide bodies 21 is different, and can be set to 10 microns, 15 microns, 20 microns, etc., according to the size of the sperm. Therefore, there are different types of glass slides 2. When testing, the appropriate glass slide 2 is selected according to the size of the sperm. The top of the glass slide body 21 is provided with a dripping inlet 23 connected to the chamber 22. The sperm preservation liquid is dripped into the dripping inlet 23, and the siphon effect of the glass slide chamber 22 is used to make the liquid enter the chamber 22 evenly. The height restriction of the chamber 22 determines that the sperm will not overlap during the movement, which improves the accuracy of the detection of sperm count and activity. The above principle is used to form a thin layer of single cells. Since the height and cleanliness of the chamber 22 are precisely controlled, the detection efficiency and accuracy of conventional sperm detection are improved, and the detection operation time is saved.

[0025] Since the glass slide 2 slides horizontally into the receiving groove 3, it is easy to slide and displace during the test; in addition, the glass slide 2 is completely loaded into the receiving groove 3 and is not easy to slide out after the test is completed; in order to make the glass slide 2 stable during the test and to slide out smoothly after the test is completed, a fixing mechanism that can fix the glass slide 2 and the auxiliary glass slide 2 is provided on the base plate 1.

[0026] like Figure 1-2 As shown, the fixing mechanism includes a T-shaped top block 5, a spring 6, and a rotating shaft 7. Slide grooves 4 are provided on both sides of the accommodating groove 3. The top block 5 is arranged on the side of the slide body 21 and is slidably adapted in the slide groove 4. The spring 6 is arranged in the slide groove 4 and arranged along its length direction. The length of the spring 6 is one quarter of the slide groove 4. One end of the spring 6 corresponds to the top block 5, and the other end is fixed to the top of the slide groove 4. The rotating shaft 7 is rotatably connected in the base plate 1 and arranged along the length direction of a plurality of slides 2. One end of the rotating shaft 7 extends outside the base plate 1. A magnetic block 1 8 and a magnetic block 2 9 are provided on both sides of the rotating shaft 7. The side of the top block 5 corresponding to the rotating shaft 7 is a magnetic body. When the magnetic block 1 8 faces the top block 5, the magnetic poles of the two are opposite, and when the magnetic block 2 9 faces the top block 5, the magnetic poles of the two are the same.

[0027] When loading the glass slide 2, first rotate the magnetic block 1 8 to the side facing the top block 5 through the rotating shaft 7, then slide the top block 5 of the glass slide 2 into the chute 4, the top block 5 slides in the chute 4 and squeezes the spring 6, when the glass slide 2 is completely loaded into the receiving groove 3, the top block 5 moves to the end of the chute 4 and adsorbs on the magnetic block 1 8, which can prevent the stability of the glass slide 2 during the detection process. When the detection is completed, rotate the rotating shaft 7 to separate the magnetic block 1 8 from the top block 5, and at the same time, the magnetic block 2 9 is facing the top block 5, so that the top block 5 loses its adsorption force, and at the same time, the top block 5 is pushed out of the chute 4 under the repulsion of the magnetic block 2 9. Under the joint action of the spring 6, at least half of the glass slide 2 is pushed out of the receiving groove 3, which is convenient for taking out the glass slide.

[0028] The device also includes a delivery mechanism that assists in delivering the dripping liquid into the dripping liquid inlet 23 .

[0029] like Figure 1-4 As shown, the conveying mechanism includes a transverse moving assembly, a support plate 10, and a funnel 11. The transverse moving assembly is arranged on the substrate 1, and can drive the support plate 10 to move along the direction of a plurality of glass slides 2. The support plate 10 is detachably connected to the transverse moving assembly. The funnel 11 is arranged on the support plate 10, and corresponds to the dripping inlet 23 up and down. After the dripping is completed, the support plate 10 can be removed from the transverse moving assembly to prevent the protruding support plate 10 and funnel 11 from affecting the detection. Keep the glass slide 2 flat during the detection.

[0030] like Figure 2 As shown, the transverse moving assembly includes a slider 12, a rack 13, a gear 14, and a connecting screw 15. The side of the base plate 1 is provided with a slide rail for the slider 12 to slide and fit. The rack 13 is arranged in the base plate 1 along the length direction of the slide rail. The gear 14 is pivoted on the slider 12 and meshes with the rack 13. When the slider 12 slides along the slide rail, it can drive the gear 14 to rotate synchronously. Conversely, when the gear 14 rotates, it can drive the slider 12 to slide synchronously on the slide rail. A screw hole is provided at the top center of the gear 14. The upper section of the connecting screw 15 is vertically slidably fitted on the support plate 10. When the connecting screw 15 rotates, it does not affect the support plate 10. The lower section is threadedly connected to the screw hole of the gear 14. The connecting screw 15 is connected to the gear 14 and can also drive the gear 14 to rotate through the connecting screw 15. When the connecting screw 15 is connected to the gear 14, the gear 14 needs to be fixed by hand, and then the connecting screw 15 is turned in. When the gear 14 loses its fixation, the connecting screw 15 rotates to drive the gear 14 to rotate synchronously. A slot 16 is vertically provided on the slider 12, and an insert block 17 that can be vertically inserted into the slot 16 is provided on the support plate 10. The support plate 10 is restricted in the slot 16 by the insert block 17, so as to prevent the support plate 10 from rotating and deviating when moving or when the connecting screw 15 is driven, and maintain the lateral stability of the support plate 10.

[0031] In order to enable the funnel 11 to move accurately to the top of the dripping inlet 23, a positioning groove 18 is provided on the substrate 1 corresponding to each glass slide 2, and a positioning column 19 adapted to the positioning groove 18 is provided at the bottom of the support plate 10. When the positioning column 19 slides into the positioning groove 18, the funnel 11 corresponds to the dripping inlet 22 up and down. When the positioning column 19 slides into the positioning groove 18, the support plate 10 will follow and drop to a certain height. Since the upper section of the connecting screw 15 and the insert block 17 have room for vertical sliding, the vertical displacement of the support plate 10 will not cause the insert block 17 to disengage from the slot 16.

[0032] like Figure 4 As shown, the thickness of the slider 4 and the end of the rotating shaft 7 extending out of the substrate 1 are both smaller than the thickness of the substrate 1, so that the upper and lower surfaces of the slide glass 2 are kept flat during detection.

[0033] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A sperm and cell counting detection slide, characterized in that: The invention comprises a substrate (1) and a plurality of glass slides (2), wherein the substrate (1) is provided with a plurality of receiving grooves (3) for the glass slides (2) to be inserted, one side of the receiving grooves (3) being open, the glass slides (2) comprising a hollow glass slide body (21), the chamber (22) in the glass slide body (21) having a height of 10 to 20 micrometers, the top of the glass slide body (21) being provided with a dripping inlet (23) connected to the chamber (22), and the substrate (1) being provided with a fixing mechanism capable of fixing the glass slide (2) and ejecting the auxiliary glass slide (2).

2. A sperm and cell counting detection slide according to claim 1, characterized in that: The fixing mechanism comprises a T-shaped top block (5), a spring (6), and a rotating shaft (7). Slide grooves (4) are provided on both sides of the accommodating groove (3). The top block (5) is arranged on the side of the slide body (21) and is slidably fitted in the slide groove (4). The spring (6) is arranged in the slide groove (4) and is arranged along the length direction thereof. One end of the spring (6) corresponds to the top block (5). The rotating shaft (7) is rotatably connected in the base plate (1) and is arranged along the length direction of a plurality of slides (2). One end of the rotating shaft (7) extends outside the base plate (1). A magnetic block 1 (8) and a magnetic block 2 (9) are provided on both sides of the rotating shaft (7). A surface of the top block (5) corresponding to the rotating shaft (7) is a magnetic body. When the magnetic block 1 (8) faces the top block (5), the magnetic poles of the two are opposite. When the magnetic block 2 (9) faces the top block (5), the magnetic poles of the two are the same.

3. A sperm and cell counting detection slide according to claim 1, characterized in that: It also includes a delivery mechanism for assisting in delivering the dripping liquid into the dripping liquid inlet (23).

4. A sperm and cell counting detection slide according to claim 3, characterized in that: The conveying mechanism comprises a transverse movement component, a support plate (10), and a funnel (11); the transverse movement component is arranged on the base plate (1) and can drive the support plate (10) to move along the direction of a plurality of glass slides (2); the support plate (10) is detachably connected to the transverse movement component; the funnel (11) is arranged on the support plate (10) and corresponds to the dripping inlet (23) up and down.

5. A sperm and cell counting detection slide according to claim 4, characterized in that: The transverse movement assembly comprises a slider (12), a rack (13), a gear (14), and a connecting screw (15). A slide rail for slidingly adapting the slider (12) is provided on the side of the base plate (1). The rack (13) is arranged in the base plate (1) along the length direction of the slide rail. The gear (14) is pivotally connected to the slider (12) and meshes with the rack (13). A screw hole is provided at the top center of the gear (14). The upper section of the connecting screw (15) is vertically slidably adapted on the support plate (10), and the lower section is threadedly connected to the screw hole of the gear (14). A slot (16) is vertically provided on the slider (12), and an insert (17) that can be vertically inserted into the slot (16) is provided on the support plate (10).

6. A sperm and cell counting detection slide according to claim 4, characterized in that: A positioning groove (18) is provided on the base plate (1) corresponding to each glass slide (2), and a positioning column (19) adapted to the positioning groove (18) is provided at the bottom of the support plate (10); when the positioning column (19) slides into the positioning groove (18), the funnel (11) corresponds to the dripping inlet (22) up and down.

7. A sperm and cell counting detection slide according to claim 5, characterized in that: The thickness of the slider (4) and the end of the rotating shaft (7) extending out of the base plate (1) is smaller than the thickness of the base plate (1).

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