Agaricus bisporus industrial cultivation method based on liquid strain

Abstract

The invention provides an agaricus bisporus industrial cultivation method based on a liquid strain, and belongs to the technical field of edible fungus cultivation. According to the method, the production cycle of the agaricus bisporus strains can be greatly shortened, and the industrial cultivation efficiency of the agaricus bisporus is effectively improved. Compared with a traditional solid strain, the agaricus bisporus liquid strain provided by the invention has a better germination effect, the bacteria running time is 2-3 days shorter than that of a solid control strain, and the yield is improved by 17.41% compared with that of a solid strain control. The spawn running period and the fruiting quality are superior to those of a traditional solid strain, and the cultivation effect is good. Meanwhile, the purity of the liquid strain is higher than that of a traditional solid strain, the recessive pollution rate of the strain is greatly reduced, and the stability of industrial cultivation is improved. According to the method, the industrial cultivation process of agaricus bisporus is simplified, complex procedures such as multi-stage solid strain propagation and inoculation in a traditional cultivation mode are reduced, the production efficiency is greatly improved, and the production cost is effectively reduced.

Description

Technical Field

[0001] The invention relates to the technical field of edible fungus cultivation, and in particular to a method for factory-based cultivation of Agaricus bisporus based on liquid strains. Background Art

[0002] Agaricus bisporus is an important edible mushroom that is widely cultivated and consumed around the world. It has delicious meat and rich nutrition. It is rich in protein, polysaccharides, multiple vitamins (such as B vitamins, provitamin D, etc.) and minerals (such as potassium, phosphorus, calcium, etc.), and has high nutritional value and health functions.

[0003] The Agaricus bisporus industry occupies a certain position in the global agricultural economy. In recent years, with the improvement of people's health awareness, the demand for natural and nutritious food has continued to increase, and the market demand for Agaricus bisporus has also grown accordingly. However, traditional Agaricus bisporus cultivation mainly relies on solid strains, which exposes many problems in the strain production and cultivation process, seriously restricting the further development of the industry.

[0004] The traditional solid strain preparation cycle is long, and the process of propagation from mother strains, original strains to cultivated strains is cumbersome, usually taking several months, which greatly affects production efficiency. At the same time, during the propagation and inoculation process of solid strains, due to the many operating links, they are easily contaminated by miscellaneous bacteria, resulting in unstable strain quality. During the cultivation process, the germination speed of solid strains is slow and the bacteria take a long time to go, which prolongs the entire cultivation cycle and faces bottlenecks in yield increase. In addition, the purity of solid strains is limited, the hidden contamination rate is high, and it is easy to cause diseases and insect pests, which has a negative impact on the yield and quality of Agaricus bisporus, and is not conducive to the stable operation of factory cultivation. Therefore, it is urgent to develop efficient and high-quality Agaricus bisporus cultivation technology. Summary of the invention

[0005] The purpose of the present invention is to provide a method for factory-based cultivation of Agaricus bisporus based on liquid spawn, which has better spawning cycle and mushroom yield and quality.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The present invention provides a method for cultivating Agaricus bisporus in a factory based on liquid spawn, comprising the following steps:

[0008] The liquid spawn of Agaricus bisporus is mixed with fermentation material and cultured to realize factory cultivation.

[0009] Preferably, the mixing method includes spraying, jetting, splashing, irrigation, atomizing spraying, dripping or pouring.

[0010] Preferably, the liquid Agaricus bisporus spawn is a liquid containing Agaricus bisporus mycelial pellets, Agaricus bisporus mycelium suspension or Agaricus bisporus mycelium culture.

[0011] Preferably, the liquid spawn concentration of Agaricus bisporus is above 150 CFU / mL.

[0012] Preferably, the spraying is achieved through a spray gun head connected to a fermentation tank.

[0013] Preferably, the discharge aperture of the spray gun head is 0.3 to 0.6 cm.

[0014] Preferably, the tank pressure of the fermentation tank is 0.03-0.08 MPa.

[0015] Preferably, the ratio of mixing the liquid Agaricus bisporus spawn with the fermentation material is: 4 to 6 L of liquid spawn per 100 kg of fermentation material.

[0016] Preferably, the mixing further includes mixing and compacting the fermentation material containing the liquid bacteria.

[0017] Preferably, the liquid spawn culture process of Agaricus bisporus includes the step of culture in a fermentation tank;

[0018] The culture parameters of the fermentation tank culture include: temperature of 22°C to 26°C, fermentation tank liquid volume of 70% to 80%, inoculation volume of 0.3% to 0.4%, initial culture pH of 5.5 to 6.0, first setting the ventilation volume to 4 to 6 L / min, then setting the ventilation volume to 7 to 10 L / min, and the total fermentation time of 5 to 10 days.

[0019] Beneficial effects of the present invention:

[0020] The factory-based cultivation method of Agaricus bisporus based on liquid spawn provided by the present invention can significantly shorten the Agaricus bisporus spawn production cycle and effectively improve the efficiency of factory-based cultivation of Agaricus bisporus. The development and promotion of the technology of the present invention will help improve the factory production efficiency of Agaricus bisporus, promote industrial upgrading, and promote the modernization of seed production and cultivation. With the country's emphasis on agricultural scientific and technological innovation, the research and application potential of Agaricus bisporus liquid spawn technology is huge. In the future, this technology will further promote the intensive and intelligent development of the Agaricus bisporus industry and promote the industry to achieve higher output value and benefits.

[0021] Compared with traditional solid strains, the Agaricus bisporus liquid strain provided by the present invention has better germination effect, the germination time is 2-3 days faster than that of the solid control strain, and the yield is 17.41% higher than that of the solid strain control. The germination cycle and mushroom yield quality are better than those of traditional solid strains, and the cultivation effect is good. At the same time, the liquid strain has higher purity than the traditional solid strain, which greatly reduces the hidden contamination rate of the strain and improves the stability of factory cultivation. The method of the present invention simplifies the factory cultivation process of Agaricus bisporus, reduces the complex processes such as multi-stage solid strain expansion and inoculation in the traditional cultivation method, greatly improves the production efficiency, and effectively reduces the production cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The process of using liquid spawn to carry out factory cultivation of Agaricus bisporus;

[0023] Figure 2 For shake flask seeds and fermenter liquid culture;

[0024] Figure 3 Spraying the liquid strain with inoculated fermentation material;

[0025] Figure 4 This is the bed surface fungus situation on the first and third days after the liquid fungus is sown;

[0026] Figure 5 This is a diagram of the mushroom bed for factory-scale cultivation of Agaricus bisporus using liquid fungus. DETAILED DESCRIPTION

[0027] The present invention provides a method for cultivating Agaricus bisporus in a factory based on liquid spawn, comprising the following steps:

[0028] The liquid spawn of Agaricus bisporus is mixed with fermentation material and cultured to realize factory cultivation.

[0029] Preferably, the mixing method includes spraying, jetting, splashing, irrigation, atomizing spraying, dripping or pouring.

[0030] Preferably, the liquid Agaricus bisporus spawn is a liquid containing Agaricus bisporus mycelial pellets, Agaricus bisporus mycelium suspension or Agaricus bisporus mycelium culture.

[0031] Preferably, the liquid spawn concentration of Agaricus bisporus is above 150 CFU / mL.

[0032] Preferably, the spraying is achieved through a spray gun head connected to a fermentation tank.

[0033] Preferably, the discharge aperture of the spray gun head is 0.3 to 0.6 cm.

[0034] Preferably, the tank pressure of the fermentation tank is 0.03-0.08 MPa.

[0035] Preferably, the ratio of mixing the liquid Agaricus bisporus spawn with the fermentation material is: 4 to 6 L of liquid spawn per 100 kg of fermentation material.

[0036] Preferably, the mixing further includes mixing and compacting the fermentation material containing the liquid bacteria.

[0037] Preferably, the liquid spawn culture process of Agaricus bisporus includes the step of culture in a fermentation tank;

[0038] The culture parameters of the fermentation tank culture include: temperature of 22°C to 26°C, fermentation tank liquid volume of 70% to 80%, inoculation volume of 0.3% to 0.4%, initial culture pH of 5.5 to 6.0, first setting the ventilation volume to 4 to 6 L / min, then setting the ventilation volume to 7 to 10 L / min, and the total fermentation time of 5 to 10 days.

[0039] In the present invention, the liquid Agaricus bisporus strain is cultured in a fermenter according to the above culture parameters. During the culture process, the environmental conditions are strictly controlled to ensure the normal growth of the liquid Agaricus bisporus strain, and obtain Agaricus bisporus pellets or liquid fermented mycelium with suitable concentration and vitality.

[0040] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0041] Example 1

[0042] Inoculation and cultivation of seed solution in shake flasks. July 9, 2024, carried out at Fujian Jinming Food Co., Ltd.

[0043] 1. Preparation of shake flask liquid culture medium. Accurately weigh the above substances according to the proportion of glucose 15g / L, yeast powder 2g / L, corn starch 8g / L, soybean powder 10g / L, potassium dihydrogen phosphate 1g / L, and magnesium sulfate 0.5g / L, dissolve in tap water, and adjust the pH value to the appropriate range (about 6.5-7.0). After constant volume, divide 600mL into 1000mL triangular flasks, seal with breathable silicone plugs, wrap the head with newspaper, and then sterilize under high pressure (121℃, 20min). Cool and set aside.

[0044] 2. Inoculation and culture. On a sterile operating table, inoculate the activated Agaricus bisporus mother culture into the shake flask culture medium. The inoculation amount is about 10 bean-sized pieces of mother culture mycelium per bottle. After the inoculation is completed, culture in a shaker in the dark (25°C, 150r / min). Cultivate for 7 days and end the shake flask seed culture.

[0045] Inoculation and cultivation of fermentation tanks. On July 16, 2024, it was carried out at Fujian Jinming Food Co., Ltd.

[0046] 1. Fermentation tank preparation. Thoroughly clean and sterilize the fermentation tank to ensure that no bacteria remain. The fermentation tank has a capacity of 200L and 150L of culture medium is filled. Its formula is similar to that of the shake flask medium or can be slightly adjusted.

[0047] 2. Inoculation process. The Agaricus bisporus shake flask seeds cultured in a shake flask for 7 days were inoculated into the fermentation tank. The inoculation volume was 600 mL. A flame ring was set at the inoculation port of the fermentation tank to ensure that no foreign bacteria entered the tank during the entire process.

[0048] 3. Fermentation tank culture. The culture parameters of the fermentation tank are set to a temperature of 22℃~26℃, an optimum culture temperature of 24℃, a fermentation tank liquid volume of 75%, an inoculation volume of 0.33%, an initial culture pH of 5.80, a ventilation volume of 5L / min on the 1st to 4th day of fermentation culture, and an aeration volume of 8~10L / min on the 5th to 7th day. The fermentation is terminated after 7 days. During the fermentation culture process, various parameters in the fermentation tank are continuously monitored, such as pH value, dissolved oxygen content, and the presence or absence of pollution. The fermentation parameters of the fermentation tank can be flexibly adjusted according to different fermentation tank models, stirring methods, and ventilation methods. After 7 days of fermentation culture, a liquid Agaricus bisporus strain with biomass and vitality that meet the requirements can be obtained.

[0049] Liquid bacteria are inoculated into fermentation materials. On July 23, 2024, it was carried out at Fujian Jinming Food Co., Ltd.

[0050] The Agaricus bisporus fermentation culture material that has undergone secondary fermentation is transferred to the loading locomotive by a forklift. The loading locomotive is connected to the conveyor belt, the conveyor belt is connected to the agitator and the pressing machine, and the other end of the pressing machine is flush with the Agaricus bisporus culture bed frame, so that the compacted fermentation culture material can be pushed to the culture bed frame.

[0051] Connect one end of the hose to the discharge port of the fermentation tank, and the other end to the spray gun head. The discharge aperture of the gun head is 0.3-0.6cm. The pressure of the fermentation tank is maintained between 0.03-0.08mpa. Aim the spray gun head at the conveyor belt. Spray 4-6L of liquid bacteria per 100kg of fermentation material as the standard. Flexible adjustment of the conveying rate of the conveyor belt ensures that a sufficient amount of liquid bacteria is inoculated. The fermentation material with liquid bacteria is mixed by the agitator, compacted by the press, and pushed to the Agaricus bisporus culture bed frame for fungus management.

[0052] Management and harvesting of mushrooms during spawning and fruiting. The temperature of the culture medium and soil covering stage should be controlled at 24-28℃, the humidity should be controlled at 90%-95%, and the CO2 concentration should be controlled at 3000-8000ppm. When inducing bud formation, attention should be paid to reducing the intensity of stimulation and slowly cooling down, preferably about 0.5℃ a day, to reduce the density of mushroom buds. During the fruiting stage, the temperature of the culture medium should be controlled at 18-21℃, the space temperature should be controlled at 17-18℃, the humidity should be controlled at 85%-90%, and the CO2 concentration should be controlled at about 1000-1500ppm. Water management should be managed according to high-yield strains. Harvest in time when the fruiting body diameter is 3.0-4.5cm.

[0053] In the above process, the process of using liquid spawn for factory cultivation of Agaricus bisporus is shown in Figure 1 ; Seed culture for shake flask seeds and liquid fermentation tanks Figure 2 ; Liquid bacteria spray inoculation fermentation material see Figure 3 ; The growth of the bed surface on the first and third days after the liquid strain was sown is shown in Figure 4 ; The bed surface diagram of the mushroom bed for industrial cultivation of Agaricus bisporus using liquid spawn is shown in Figure 5 .

[0054] Through the above specific implementation methods, the use of Agaricus bisporus liquid spawn for factory-based cultivation and production of Agaricus bisporus can be effectively achieved, thereby achieving the purpose of the present invention.

[0055] The traditional solid strain production method is a three-level propagation system, the culture medium is wheat grains, and the sowing method is particle broadcasting. The comparison results are shown in Table 1:

[0056] Table 1 Comparison results between the present invention and solid strains

[0057]

[0058] It can be seen from the above embodiments that, compared with the traditional solid strains, the germination effect of the Agaricus bisporus liquid strains is good, the germination time is 2-3 days faster than the solid control strains, and the yield is 17.41% higher than the solid strain control. The germination cycle and the quality of mushroom production are better than those of the traditional solid strains, and the cultivation effect is good. At the same time, the liquid strains are higher in purity than the traditional solid strains, which greatly reduces the hidden contamination rate of the strains and improves the stability of factory cultivation. The method of the present invention simplifies the factory cultivation process of Agaricus bisporus, reduces the complex processes such as multi-stage solid strain expansion and inoculation in the traditional cultivation method, greatly improves the production efficiency, and effectively reduces the production cost.

[0059] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for the factory cultivation of Agaricus bisporus based on liquid spawn, characterized in that: The following steps are involved: The liquid spawn of Agaricus bisporus is mixed with fermentation material and cultured to realize factory cultivation.

2. The method for industrial cultivation of Agaricus bisporus according to claim 1, characterized in that: The mixing methods include spraying, jetting, splashing, irrigation, atomizing spraying, dripping or pouring.

3. The method for industrial cultivation of Agaricus bisporus according to claim 1, characterized in that: The liquid mushroom spawn of Agaricus bisporus is a liquid containing Agaricus bisporus mycelium pellets, Agaricus bisporus mycelium suspension or Agaricus bisporus mycelium culture.

4. The method for industrial cultivation of Agaricus bisporus according to claim 1, characterized in that: The liquid spawn concentration of Agaricus bisporus is above 150 CFU / mL.

5. The method for industrial cultivation of Agaricus bisporus according to claim 2, characterized in that: The injection is achieved by connecting a injection gun head to the fermentation tank.

6. The method for industrial cultivation of Agaricus bisporus according to claim 5, characterized in that: The discharge aperture of the spray gun head is 0.3-0.6 cm.

7. The method for industrial cultivation of Agaricus bisporus according to claim 5, characterized in that: The tank pressure of the fermentation tank is 0.03-0.08 MPa.

8. The method for industrial cultivation of Agaricus bisporus according to claim 1, characterized in that: The ratio of mixing the liquid mushroom strain of Agaricus bisporus with the fermentation material is: 4 to 6 L of the liquid mushroom strain is mixed with every 100 kg of the fermentation material.

9. The method for industrial cultivation of Agaricus bisporus according to claim 1, characterized in that: After the mixing, the fermentation material containing the liquid bacteria is mixed and compacted.

10. The method for industrial cultivation of Agaricus bisporus according to any one of claims 1 to 9, characterized in that: The liquid spawn culture process of Agaricus bisporus includes the step of culture in a fermentation tank; The culture parameters of the fermentation tank culture include: temperature of 22°C to 26°C, fermentation tank liquid volume of 70% to 80%, inoculation volume of 0.3% to 0.4%, initial culture pH of 5.5 to 6.0, first setting the ventilation volume to 4 to 6 L / min, then setting the ventilation volume to 7 to 10 L / min, and the total fermentation time of 5 to 10 days.

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