A light quality matching method for improving the lignification degree and rooting rate of asparagus tissue seedling

Abstract

The application belongs to the technical field of Chinese medicinal material seedling raising, and particularly relates to a light quality matching method for improving the lignification degree and rooting rate of asparagus seedlings. Compared with the prior art of directly using natural light or using a conventional tissue culture LED lamp as a light source, the asparagus leaf unfolding time can be effectively shortened, so that the lignification degree and rooting rate of the asparagus seedlings are improved.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of Chinese medicinal material seedling cultivation, and particularly relates to a light quality matching method for improving the lignification degree and rooting rate of Asparagus cochinchinensis tissue culture seedlings. BACKGROUND

[0002] Asparagus cochinchinensis in Neijiang is a local medicinal material and landmark product in Neijiang, and has great differences in morphology, composition and propagation mode from Asparagus cochinchinensis in production areas such as Guangxi and Yunnan. Asparagus cochinchinensis in Neijiang has excellent quality, higher contents of polysaccharides, amino acids and extractives, and lower content of saponin ingredients, tastes sweet in mouth and has no bitter taste in mouth, has a long history of consumption, has a wide application prospect, is an important variety of Asparagus cochinchinensis in the use of food and medicine, and has high development potential of food and health products. In recent years, Neijiang City has vigorously developed traditional Chinese medicine and health industry, created "Chinese Asparagus cochinchinensis hometown", and promoted the inheritance and innovation development of traditional Chinese medicine in Neijiang. However, the seed setting rate of Asparagus cochinchinensis in Neijiang is low, the germination rate is low, the traditional propagation mode is to rely on propagation by division, the mother plant needs to grow for 3-4 years, and propagation is carried out in the harvest season. Usually, 3-5 plants can be propagated from each plant, the propagation time is long, the efficiency is low, and the development of Asparagus cochinchinensis industry in Neijiang is greatly limited. The establishment of Asparagus cochinchinensis tissue culture and rapid propagation technology in Neijiang can solve the problem of shortage of seedlings in production. However, the light source commonly used in Asparagus cochinchinensis tissue culture is white LED lamp, the light quantum density is low under the same light intensity, and there is no light source system developed for Asparagus cochinchinensis in Neijiang at present. Using the traditional light system and the existing tissue culture technology, the lignification degree of Asparagus cochinchinensis tissue culture seedlings before rooting is low, the step of strengthening seedlings needs to be increased, the growth time is long, and the rooting rate is low, which greatly increases the production cost of Asparagus cochinchinensis tissue culture seedlings in Neijiang and affects the development of Asparagus cochinchinensis industry. SUMMARY

[0003] In order to overcome the above technical problems, the application provides a light quality matching method for improving the lignification degree and rooting rate of Asparagus cochinchinensis tissue culture seedlings, which improves the lignification degree and rooting rate of Asparagus cochinchinensis tissue culture seedlings by improving the light source of Asparagus cochinchinensis in the propagation stage and shortening the time of Asparagus cochinchinensis leaflet unfolding.

[0004] In order to achieve the above purpose, the technical scheme of the application discloses a light quality matching method for improving the lignification degree and rooting rate of Asparagus cochinchinensis tissue culture seedlings, which comprises the following steps:

[0005] The following light quality matching treatment is used in the propagation stage of Asparagus cochinchinensis tissue culture seedlings:

[0006] 380-399 nm: 0.05%-0.15%;

[0007] 400-499 nm: 20.5%-30.0%;

[0008] 500-599 nm: 30.05%-43.5%;

[0009] Wavelength 600-699 nm: 29.05%-40.0%;

[0010] Wavelength 700-780 nm: 1.50%-2.75%;

[0011] The asparagus rooting stage is treated with the following light quality matching:

[0012] Wavelength 380-399 nm: 0.04%-0.14%;

[0013] Wavelength 400-499 nm: 17.0%-25.0%;

[0014] Wavelength 500-599 nm: 35.5%-43.5%;

[0015] Wavelength 600-699 nm: 30.5%-41.5%;

[0016] Wavelength 700-780 nm: 2.4%-4.0%.

[0017] Preferably, the light quality matching method comprises the following steps:

[0018] The asparagus tissue culture seedling proliferation stage is treated with the following light quality matching:

[0019] Wavelength 380-399 nm: 0.07%-0.13%;

[0020] Wavelength 400-499 nm: 22.36%-29.31%;

[0021] Wavelength 500-599 nm: 36.6%-43.25%;

[0022] Wavelength 600-699 nm: 29.36%-34.92%;

[0023] Wavelength 700-780 nm: 1.84%-2.02%;

[0024] The asparagus rooting stage is treated with the following light quality matching:

[0025] Wavelength 380-399 nm: 0.04%-0.10%;

[0026] Wavelength 400-499 nm: 18.04%-24.16%;

[0027] Wavelength 500-599 nm: 36.06%-42.98%;

[0028] Wavelength 600-699 nm: 33.78%-39.8%;

[0029] Wavelength 700-780nm: 2.33%-2.69%.

[0030] Further, the light intensity of the propagation stage and the rooting stage is 2000-4000lx.

[0031] Further, the light quantum density of the propagation stage is 90-140umol / m 2 / s.

[0032] Further, the light quantum density of the rooting stage is 40-80umol / m 2 / s.

[0033] Further, the red blue light ratio of the asparagus tissue culture seedling propagation stage is 0.8-1.5, and the red light and far red light ratio is 10.0-20.0.

[0034] Further, the red blue light ratio of the asparagus rooting stage is 2.0-3.0, and the red light and far red light ratio is 7.0-15.0.

[0035] Compared with the prior art, the present application has the following beneficial effects: by improving the light source intensity, light quality ratio and the like of asparagus in the propagation stage and the rooting stage after propagation, compared with the prior art which directly uses natural light or uses white light as the light source, the asparagus leaflet unfolding time can be effectively shortened, so that the asparagus tissue culture seedling lignification degree and rooting rate are improved. DETAILED DESCRIPTION

[0036] The technical solutions in the embodiments of the present application will be clearly and completely described below in combination with the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0037] Unless otherwise specifically stated, the relative arrangement of parts and steps, numerical expressions, and numerical values set forth in the various examples herein are not limiting but merely exemplary. Any technical, methodological, and / or other descriptions set forth in the above description are not necessarily indicative of patentable scope. Any reference to claim elements in the detailed description is not exhaustive of the scope of the technology. Accordingly, other examples are within the scope of the examples.

[0038] Unless otherwise specifically stated, the meanings of the scientific and technical terms in the present specification are the same as those generally understood by those skilled in the art, but if there is a conflict, the definitions in the present specification shall prevail.

[0039] Effects of different light quality combinations on bud formation and lignification degree of Asparagus cochinchinensis cv. Neijiang tissue culture seedlings

[0040] Experimental method: Neijiang Asparagus cochinchinensis cv. Neijiang tissue culture seedlings were inoculated on proliferation medium and placed in different light quality combinations of LED lamps. The commonly used white light LED lamp was used as a control to observe the effects of different LED light sources on the growth indicators, growth state and lignification degree of Neijiang Asparagus cochinchinensis cv. Neijiang tissue culture seedlings. The proliferation medium formula was: MS + 1.2 mg / L 6-Ba + 0.1 mg / L NAA + 0.2 mg / L IAA + 0.2 mg / L KT, sucrose 30 g / L, agar 5.5 g / L.

[0041] The spectral data of different LED light sources are shown in Table 1. The spectral data of the front, middle and rear sections of the same light source were measured and the average value was calculated:

[0042] Table 1 Spectral treatment of different LED light sources

[0043]

[0044] After 40 days of induction of Neijiang Asparagus cochinchinensis cv. Neijiang tissue culture seedlings, the bud length, base thickness, bud number, bud number, growth state, lignification degree and leaf-like expansion were counted. The effects of different light qualities on bud formation and lignification degree of Neijiang Asparagus cochinchinensis cv. Neijiang are shown in Table 2.

[0045] Table 2 Effects of different light qualities on growth and lignification degree of Neijiang Asparagus cochinchinensis cv. Neijiang with clumps

[0046]

[0047] Table 3 Effects of different light qualities on growth and lignification degree of Neijiang Asparagus cochinchinensis cv. Neijiang without clumps

[0048]

[0049] The results showed that different light qualities had a greater effect on the growth indicators, lignification degree and leaf-like expansion of Neijiang Asparagus cochinchinensis cv. Neijiang with clumps. Light quality 1 was beneficial to the growth of bud length, light quality 4 was not conducive to the proliferation growth of base clumps and stem thickness, light quality 3 had an inhibitory effect on the growth of bud number and bud number, and light quality 4 was conducive to the growth of more buds on the same bud, but not conducive to the differentiation of more bud numbers at the base. Under the five light qualities, the overall growth of Neijiang Asparagus cochinchinensis cv. Neijiang with clumps was good. Under light quality 1 and light quality 4, the lignification degree of Neijiang Asparagus cochinchinensis cv. Neijiang with clumps was greatly improved, and the leaf-like was completely expanded. This type of material is suitable for rooting of Neijiang Asparagus cochinchinensis cv. Neijiang in the later stage.

[0050] Different light quality has a great influence on the growth index, lignification degree and leaf-like expansion degree of Asparagus cochinchinensis not with clump seedlings. Light quality 1 can greatly promote the growth of bud length, base stem diameter and leaf-like expansion, but the overall growth state of the plant is poor. Light quality 2 can promote the complete expansion of the leaf-like, but has an inhibitory effect on the overall growth of Asparagus cochinchinensis not with clump seedlings. Light quality 3 can promote the growth of base stem diameter and bud number, and the growth state of the plant is good, and the leaf-like expansion is occasionally observed. Light quality 4 can promote the expansion of the leaf-like and inhibit the growth of the bud number, and the growth state of the seedling is good.

[0051] Example 2 Influence of different light quality collocation on the rooting rate of Asparagus cochinchinensis tissue culture seedlings

[0052] Asparagus cochinchinensis tissue culture seedlings with high leaf-like differentiation degree in the proliferation stage are inoculated into a rooting culture medium and placed in an LED lamp with different light quality collocation, and a commonly used white light LED lamp is used as a control to observe the influence of different LED light sources on the rooting rate and growth state of Asparagus cochinchinensis rooting seedlings (the spectral data of different light sources are shown in Table 1). The rooting culture medium is formulated as follows: 1 / 4MS (1 / 4 iron salt) + IBA 0.5 mg / L + IAA 0.5 mg / L + KT 0.05 mg / L + CCC 4.0 mg / L + banana 50 g / L, sucrose 20 g / L and agar 5.5 g / L.

[0053] After 60 days of culture, the number of roots, root length and rooting rate are counted; the influence of different light qualities on the rooting rate of Asparagus cochinchinensis is shown in Table 3.

[0054] Table 4 Influence of different light qualities on the rooting rate of Asparagus cochinchinensis

[0055]

[0056] As shown in Table 4, different light quality collocation has an influence on the growth and rooting rate of Asparagus cochinchinensis. The growth state of Asparagus cochinchinensis under light quality 2 is good, and the rooting rate is the highest, reaching 65%, which is much higher than 43.3% of the conventional light source. Light quality 1 and light quality 4 collocation are also beneficial to improve the seedling growth state and rooting rate of Asparagus cochinchinensis.

[0057] In summary, in the factory production of Asparagus cochinchinensis tissue culture seedlings, light quality 1 is used in the proliferation stage and light quality 4 is used in the rooting stage, which can shorten the growth time of Asparagus cochinchinensis tissue culture seedlings to the greatest extent, so as to shorten the production cycle of Asparagus cochinchinensis tissue culture seedlings and save the production cost.

[0058] The above is merely a specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any changes or replacements within the technical range disclosed by the present application can be easily thought of by those skilled in the art, which should be covered in the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A method for adjusting light quality to improve the lignification degree and rooting rate of asparagus tissue culture seedlings, characterized in that, Includes the following steps: The following light quality treatment was used during the propagation stage of asparagus tissue culture seedlings: Wavelengths of 380-399nm: 0.05%-0.15%; Wavelengths of 400-499nm: 20.5%-30.0%; Wavelengths of 500-599nm: 30.05%-43.5%; Wavelengths of 600-699nm: 29.05%-40.0%; Wavelengths of 700-780nm: 1.50%-2.75%; The following light quality treatments were used during the rooting stage of asparagus: Wavelengths of 380-399nm: 0.04%-0.14%; Wavelengths of 400-499nm: 17.0%-25.0%; Wavelengths of 500-599nm: 35.5%-43.5%; Wavelengths of 600-699nm: 30.5%-41.5%; Wavelengths of 700-780nm: 2.4%-4.0%; The light intensity for both the proliferation and rooting stages is 2000-4000 lx. The quantum density during the proliferation phase is 90-140 μmol / m². 2 / s; the photon density during the rooting stage is 40-80 μmol / m 2 / s.

2. The light quality matching method according to claim 1, characterized in that, Includes the following steps: The following light quality treatment was used during the propagation stage of asparagus tissue culture seedlings: Wavelengths of 380-399nm: 0.07%-0.13%; Wavelengths of 400-499nm: 22.36%-29.31%; Wavelengths of 500-599nm: 36.6%-43.25%; Wavelengths of 600-699nm: 29.36%-34.92%; Wavelengths of 700-780nm: 1.84%-2.02%; The following light quality treatments were used during the rooting stage of asparagus: Wavelengths of 380-399nm: 0.04%-0.10%; Wavelengths of 400-499nm: 18.04%-24.16%; Wavelengths of 500-599nm: 36.06%-42.98%; Wavelengths of 600-699nm: 33.78%-39.8%; Wavelengths of 700-780nm: 2.33%-2.69%.

3. The planting method according to claim 1, characterized in that, The red-to-blue light ratio during the propagation stage of the asparagus tissue culture seedlings was 0.8-1.5, and the red-to-far-red light ratio was 10.0-20.

0.

4. The planting method according to claim 1, characterized in that... The red-to-blue light ratio during the rooting stage of asparagus is 2.0-3.0, and the red-to-far-red light ratio is 7.0-15.0.

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